Rmu_sc0001227.1_g000033

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001227.1
OG0000830
Physical Location & Seq
Reverse (-)
118061 .. 122537
4477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001227.1_g000033.1.cds

Sequence Viewer

Length: 1659 bp
atgctctcctccgaccaccaaaagaccccctcccgtggtggctggaacgccgccattttcatcatcttcgtcgaggttgcagagaggtttgcttactatggcctgataggtaacctcatcatgtacctcaccaatgagctccaccagcccacggctgccgccgccaagaacgtcaacttgtggatcggcctctcctccctcttccccatcctcggggccatcgtcgccgattcttacctcggccgcttcaagacaatcatatactccacctgcatttatttcctgggaatggttatgctgtgcttatctgcttcggtggttcctcggcgttatcgtgaaggggcatacttcgtggggctttacattctggcggtgggagaaggcggccacaagccttgcgtgcagaccttcgccgccgaccagtttgaggaggagactgatgaggacaaggaggctaagagctcattcttcaactggtggtatttggctatagttgtcaccgctactgctgccacacttggtatcatctatattcaggataatgtgagctgggtgctgagctatagtgtcttggcagtggtaatagcagcaggcctcctattatttgtactcggaatccgaaggtatcggaacgacgataatctgttggggagccccttcacgacggtggcacaagtatttgttgcggcagcttggaagtggcgcgtaaagacgggtcgtcggaatgatgttgtctactacggtgatgatgagggtgagagcagaggggttcactccgagcacaagacattggctcgtactaaacaattcagatttctggacaaggcaatgattattgacgaacatgatgcgtcaagcaaaatgaggaatccttggaggttgtgctctctaaatcaagtggaagaagtgaagcttgttgtccgtctaattcccatatggttgagctgcctaatgtttcacgcagttcaatctcaactccacacctacttcatcaaacaaggttccacgatgatcagatccatcggacccaactttcggttcccccctgcatcacttcaagccctagttggcattgtgatcgtatctaccgttccaatctacgaccggattcttgtcccgacggtaagaaagttcacaggacatcccgcgggcataacagtcctccaacgaattggtgtgggcctattcttgtccataatcaacatggttgtggcagctctagtggaagctaaaagggttggtgttgcaagagaatataacctcatggataacccaaaagcaatggtaccaatcagtgtctggtggttactcccacaatactttatcactggtttgtctgatgcattcaccgttataggactccaagagttgttctatgatcaaatgccagatgcaatgagaagcttgggagctgctgcatacattagtattcttggagttggaagctttcttagcagtttcatcatatccttggtggaaactattaccagaaactctggtgagccgtggttaggtaacaatctcaaccgcgcacaccttgatgacttctactgggtgcttgcaggactaagcgctctatttttcggtgtttatgtgtgggttgcaaagcgatttgtttacaagaaagttgtaggggaagcatcaagcggggaggtgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

552

Amino Acids

61.68

Weight (kDa)

8.73

Isoelectric Point (pI)

31.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 278
Acc36I ACCTGC 1 cut(s) 278
Acc65I GGTACC 1 cut(s) 1285
AccB1I GGYRCC 1 cut(s) 1285
AccI GTMKAC 1 cut(s) 735
AccII CGCG 3 cut(s) 705, 1148, 1530
AclWI GGATC 2 cut(s) 191, 1011
AcoI YGGCCR 2 cut(s) 241, 385
AfaI GTAC 4 cut(s) 125, 609, 799, 1287
AfeI AGCGCT 1 cut(s) 1573
AfiI CCNNNNNNNGG 8 cut(s) 35, 151, 212, 213, 289, 427, 1035, 1511
AgsI TTSAA 4 cut(s) 250, 472, 968, 1058
AhdI GACNNNNNGTC 1 cut(s) 717
AjnI CCWGG 1 cut(s) 282
AjuI GAANNNNNNNTTGG 2 cut(s) 1022, 1054
AleI CACNNNNGTG 1 cut(s) 665
Alw21I GWGCWC 4 cut(s) 141, 464, 783, 887
Alw26I GTCTC 1 cut(s) 428
AlwI GGATC 2 cut(s) 191, 1011
AlwNI CAGNNNCTG 1 cut(s) 1299
Ama87I CYCGRG 1 cut(s) 212
Aor51HI AGCGCT 1 cut(s) 1573
AoxI GGCC 7 cut(s) 100, 187, 216, 241, 385, 592, 1180
ApeKI GCWGC 8 cut(s) 155, 509, 587, 689, 945, 1214, 1412, 1415
ArsI GACNNNNNNTTYG 2 cut(s) 1017, 1049
Asp718I GGTACC 1 cut(s) 1285
AspLEI GCGC 3 cut(s) 705, 1532, 1574
AspS9I GGNCC 3 cut(s) 216, 1025, 1180
AsuHPI GGTGA 6 cut(s) 121, 490, 755, 767, 1339, 1511
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 1 cut(s) 1025
BanI GGYRCC 1 cut(s) 1285
BanII GRGCYC 3 cut(s) 141, 464, 656
Bbv12I GWGCWC 4 cut(s) 141, 464, 783, 887
BbvI GCAGC 8 cut(s) 142, 496, 599, 701, 932, 1226, 1399, 1402
BccI CCATC 3 cut(s) 215, 227, 1028
BceAI ACGGC 2 cut(s) 168, 1489
BcgI CGANNNNNNTGC 4 cut(s) 830, 864, 1060, 1094
BciT130I CCWGG 1 cut(s) 284
BclI TGATCA 2 cut(s) 1011, 1378
BcoDI GTCTC 1 cut(s) 428
BfaI CTAG 2 cut(s) 1064, 1220
BfmI CTRYAG 2 cut(s) 489, 562
BfoI RGCGCY 1 cut(s) 1575
BfuAI ACCTGC 1 cut(s) 278
BlpI GCTNAGC 1 cut(s) 557
Bme1390I CCNGG 1 cut(s) 284
Bme18I GGWCC 1 cut(s) 1025
BmeRI GACNNNNNGTC 1 cut(s) 717
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 3 cut(s) 216, 1025, 1180
BmiI GGNNCC 7 cut(s) 217, 321, 653, 1003, 1027, 1040, 1287
BmrFI CCNGG 1 cut(s) 284
BmrI ACTGGG 1 cut(s) 1561
BmsI GCATC 5 cut(s) 838, 1058, 1330, 1381, 1649
BmuI ACTGGG 1 cut(s) 1561
Bpu1102I GCTNAGC 1 cut(s) 557
BsaWI WCCGGW 1 cut(s) 1104
Bsc4I CCNNNNNNNGG 8 cut(s) 35, 151, 212, 213, 289, 427, 1035, 1511
Bse1I ACTGG 4 cut(s) 421, 479, 1333, 1556
Bse3DI GCAATG 3 cut(s) 834, 1287, 1401
BseBI CCWGG 1 cut(s) 284
BseGI GGATG 2 cut(s) 207, 1141
BseLI CCNNNNNNNGG 8 cut(s) 35, 151, 212, 213, 289, 427, 1035, 1511
BseMI GCAATG 3 cut(s) 834, 1287, 1401
BseMII CTCAG 1 cut(s) 548
BseNI ACTGG 4 cut(s) 421, 479, 1333, 1556
BseRI GAGGAG 3 cut(s) 184, 443, 446
BseX3I CGGCCG 1 cut(s) 241
BseXI GCAGC 8 cut(s) 142, 496, 599, 701, 932, 1226, 1399, 1402
BseYI CCCAGC 1 cut(s) 549
BsgI GTGCAG 1 cut(s) 422
Bsh1236I CGCG 3 cut(s) 705, 1148, 1530
Bsh1285I CGRYCG 2 cut(s) 244, 1105
BshFI GGCC 7 cut(s) 102, 189, 218, 243, 387, 594, 1182
BshNI GGYRCC 1 cut(s) 1285
BsiEI CGRYCG 2 cut(s) 244, 1105
BsiHKAI GWGCWC 4 cut(s) 141, 464, 783, 887
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 1 cut(s) 1105
BslFI GGGAC 1 cut(s) 1100
BslI CCNNNNNNNGG 8 cut(s) 35, 151, 212, 213, 289, 427, 1035, 1511
BsmAI GTCTC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 1100
BsmI GAATGC 1 cut(s) 1343
BsnI GGCC 7 cut(s) 102, 189, 218, 243, 387, 594, 1182
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 5 cut(s) 141, 464, 656, 783, 887
Bsp143I GATC 5 cut(s) 183, 1011, 1016, 1077, 1378
Bsp1720I GCTNAGC 1 cut(s) 557
BspANI GGCC 7 cut(s) 102, 189, 218, 243, 387, 594, 1182
BspCNI CTCAG 1 cut(s) 549
BspFNI CGCG 3 cut(s) 705, 1148, 1530
BspLI GGNNCC 7 cut(s) 217, 321, 653, 1003, 1027, 1040, 1287
BspMI ACCTGC 1 cut(s) 278
BspPI GGATC 2 cut(s) 191, 1011
BspT107I GGYRCC 1 cut(s) 1285
BsrDI GCAATG 3 cut(s) 834, 1287, 1401
BsrI ACTGG 4 cut(s) 421, 479, 1333, 1556
BssMI GATC 5 cut(s) 183, 1011, 1016, 1077, 1378
BssT1I CCWWGG 2 cut(s) 872, 1470
Bst2UI CCWGG 1 cut(s) 284
Bst4CI ACNGT 6 cut(s) 667, 743, 1090, 1123, 1159, 1351
Bst6I CTCTTC 1 cut(s) 206
BstC8I GCNNGC 4 cut(s) 401, 592, 1150, 1560
BstDEI CTNAG 4 cut(s) 456, 557, 1451, 1568
BstDSI CCRYGG 4 cut(s) 34, 150, 1146, 1505
BstEII GGTNACC 1 cut(s) 110
BstF5I GGATG 2 cut(s) 207, 1141
BstFNI CGCG 3 cut(s) 705, 1148, 1530
BstH2I RGCGCY 1 cut(s) 1575
BstHHI GCGC 3 cut(s) 705, 1532, 1574
BstKTI GATC 5 cut(s) 186, 1014, 1019, 1080, 1381
BstMAI GTCTC 1 cut(s) 428
BstMBI GATC 5 cut(s) 183, 1011, 1016, 1077, 1378
BstMCI CGRYCG 2 cut(s) 244, 1105
BstMWI GCNNNNNNNGC 6 cut(s) 145, 161, 224, 400, 509, 1452
BstNI CCWGG 1 cut(s) 284
BstPI GGTNACC 1 cut(s) 110
BstSCI CCNGG 1 cut(s) 282
BstSFI CTRYAG 2 cut(s) 489, 562
BstUI CGCG 3 cut(s) 705, 1148, 1530
BstV1I GCAGC 8 cut(s) 142, 496, 599, 701, 932, 1226, 1399, 1402
BstX2I RGATCY 1 cut(s) 1016
BstXI CCANNNNNNTGG 1 cut(s) 1172
BstYI RGATCY 1 cut(s) 1016
BstZI CGGCCG 1 cut(s) 241
BsuRI GGCC 7 cut(s) 102, 189, 218, 243, 387, 594, 1182
BtgI CCRYGG 4 cut(s) 34, 150, 1146, 1505
BtsCI GGATG 2 cut(s) 207, 1141
BtsI GCAGTG 1 cut(s) 582
BtsIMutI CAGTG 3 cut(s) 582, 1300, 1326
BveI ACCTGC 1 cut(s) 278
Cac8I GCNNGC 4 cut(s) 401, 592, 1150, 1560
CaiI CAGNNNCTG 1 cut(s) 1299
CfoI GCGC 3 cut(s) 705, 1532, 1574
Cfr13I GGNCC 3 cut(s) 216, 1025, 1180
Cfr42I CCGCGG 1 cut(s) 1149
CseI GACGC 1 cut(s) 840
Csp6I GTAC 4 cut(s) 124, 608, 798, 1286
CspCI CAANNNNNGTGG 2 cut(s) 377, 412
CviAII CATG 4 cut(s) 121, 845, 1204, 1264
CviQI GTAC 4 cut(s) 124, 608, 798, 1286
DdeI CTNAG 4 cut(s) 456, 557, 1451, 1568
DpnI GATC 5 cut(s) 185, 1013, 1018, 1079, 1380
DpnII GATC 5 cut(s) 183, 1011, 1016, 1077, 1378
DriI GACNNNNNGTC 1 cut(s) 717
EaeI YGGCCR 2 cut(s) 241, 385
EagI CGGCCG 1 cut(s) 241
Eam1104I CTCTTC 1 cut(s) 206
Eam1105I GACNNNNNGTC 1 cut(s) 717
EarI CTCTTC 1 cut(s) 206
Ecl136II GAGCTC 2 cut(s) 139, 462
EclXI CGGCCG 1 cut(s) 241
Eco130I CCWWGG 2 cut(s) 872, 1470
Eco147I AGGCCT 1 cut(s) 594
Eco24I GRGCYC 3 cut(s) 141, 464, 656
Eco47I GGWCC 1 cut(s) 1025
Eco47III AGCGCT 1 cut(s) 1573
Eco52I CGGCCG 1 cut(s) 241
Eco53kI GAGCTC 2 cut(s) 139, 462
Eco88I CYCGRG 1 cut(s) 212
Eco91I GGTNACC 1 cut(s) 110
EcoICRI GAGCTC 2 cut(s) 139, 462
EcoO65I GGTNACC 1 cut(s) 110
EcoRII CCWGG 1 cut(s) 282
EcoT14I CCWWGG 2 cut(s) 872, 1470
EcoT22I ATGCAT 1 cut(s) 1345
EcoT38I GRGCYC 3 cut(s) 141, 464, 656
ErhI CCWWGG 2 cut(s) 872, 1470
FaeI CATG 4 cut(s) 124, 848, 1207, 1267
FalI AAGNNNNNCTT 2 cut(s) 897, 929
FaqI GGGAC 1 cut(s) 1100
FatI CATG 4 cut(s) 120, 844, 1203, 1263
FauI CCCGC 3 cut(s) 1141, 1153, 1640
FauNDI CATATG 1 cut(s) 935
FbaI TGATCA 2 cut(s) 1011, 1378
FblI GTMKAC 1 cut(s) 735
FokI GGATG 2 cut(s) 194, 1128
FriOI GRGCYC 3 cut(s) 141, 464, 656
FspBI CTAG 2 cut(s) 1064, 1220
GlaI GCGC 3 cut(s) 704, 1531, 1573
GsaI CCCAGC 1 cut(s) 553
HaeII RGCGCY 1 cut(s) 1575
HaeIII GGCC 7 cut(s) 102, 189, 218, 243, 387, 594, 1182
HapII CCGG 1 cut(s) 1105
HgaI GACGC 1 cut(s) 840
HhaI GCGC 3 cut(s) 705, 1532, 1574
Hin1II CATG 4 cut(s) 124, 848, 1207, 1267
Hin6I GCGC 3 cut(s) 703, 1530, 1572
HinP1I GCGC 3 cut(s) 703, 1530, 1572
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HindIII AAGCTT 3 cut(s) 911, 1402, 1444
HinfI GANTC 5 cut(s) 230, 615, 868, 1108, 1359
HpaII CCGG 1 cut(s) 1105
HphI GGTGA 6 cut(s) 121, 490, 755, 767, 1339, 1511
Hpy166II GTNNAC 5 cut(s) 175, 736, 772, 1134, 1618
Hpy188III TCNNGA 6 cut(s) 250, 335, 536, 661, 818, 1117
Hpy8I GTNNAC 5 cut(s) 175, 736, 772, 1134, 1618
Hpy99I CGWCG 6 cut(s) 74, 227, 638, 667, 723, 1123
HpyAV CCTTC 5 cut(s) 332, 374, 418, 615, 667
HpyCH4III ACNGT 6 cut(s) 667, 743, 1090, 1123, 1159, 1351
HpyCH4IV ACGT 1 cut(s) 171
HpyF10VI GCNNNNNNNGC 6 cut(s) 145, 161, 224, 400, 509, 1452
HpyF3I CTNAG 4 cut(s) 456, 557, 1451, 1568
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 4 cut(s) 124, 848, 1207, 1267
HspAI GCGC 3 cut(s) 703, 1530, 1572
KpnI GGTACC 1 cut(s) 1289
Ksp22I TGATCA 2 cut(s) 1011, 1378
KspI CCGCGG 1 cut(s) 1149
Kzo9I GATC 5 cut(s) 183, 1011, 1016, 1077, 1378
LmnI GCTCC 3 cut(s) 144, 651, 1409
Lsp1109I GCAGC 8 cut(s) 142, 496, 599, 701, 932, 1226, 1399, 1402
LweI GCATC 5 cut(s) 838, 1058, 1330, 1381, 1649
MaeI CTAG 2 cut(s) 1064, 1220
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 4 cut(s) 110, 496, 1305, 1514
MalI GATC 5 cut(s) 185, 1013, 1018, 1079, 1380
MboI GATC 5 cut(s) 183, 1011, 1016, 1077, 1378
MboII GAAGA 4 cut(s) 58, 193, 460, 914
MflI RGATCY 1 cut(s) 1016
MhlI GDGCHC 5 cut(s) 141, 464, 656, 783, 887
MluCI AATT 3 cut(s) 806, 927, 1170
MlyI GAGTC 1 cut(s) 1353
MmeI TCCRAC 4 cut(s) 36, 701, 1189, 1420
Mph1103I ATGCAT 1 cut(s) 1345
MslI CAYNNNNRTG 3 cut(s) 665, 1208, 1539
MspA1I CMGCKG 1 cut(s) 1148
MspI CCGG 1 cut(s) 1105
MspR9I CCNGG 1 cut(s) 284
Mva1269I GAATGC 1 cut(s) 1343
MvaI CCWGG 1 cut(s) 284
MvnI CGCG 3 cut(s) 705, 1148, 1530
MwoI GCNNNNNNNGC 6 cut(s) 145, 161, 224, 400, 509, 1452
NdeI CATATG 1 cut(s) 935
NdeII GATC 5 cut(s) 183, 1011, 1016, 1077, 1378
NlaIII CATG 4 cut(s) 124, 848, 1207, 1267
NlaIV GGNNCC 7 cut(s) 217, 321, 653, 1003, 1027, 1040, 1287
NmeAIII GCCGAG 2 cut(s) 219, 304
NmuCI GTSAC 1 cut(s) 496
NsiI ATGCAT 1 cut(s) 1345
OliI CACNNNNGTG 1 cut(s) 665
PaqCI CACCTGC 1 cut(s) 278
PceI AGGCCT 1 cut(s) 594
PcsI WCGNNNNNNNCGW 3 cut(s) 219, 331, 1086
PctI GAATGC 1 cut(s) 1343
PfeI GAWTC 4 cut(s) 230, 615, 868, 1108
PleI GAGTC 1 cut(s) 1353
PpsI GAGTC 1 cut(s) 1353
Psp124BI GAGCTC 2 cut(s) 141, 464
Psp6I CCWGG 1 cut(s) 282
PspEI GGTNACC 1 cut(s) 110
PspFI CCCAGC 1 cut(s) 549
PspGI CCWGG 1 cut(s) 282
PspN4I GGNNCC 7 cut(s) 217, 321, 653, 1003, 1027, 1040, 1287
PspPI GGNCC 3 cut(s) 216, 1025, 1180
PsrI GAACNNNNNNTAC 2 cut(s) 1074, 1106
PstNI CAGNNNCTG 1 cut(s) 1299
PsuI RGATCY 1 cut(s) 1016
RsaI GTAC 4 cut(s) 125, 609, 799, 1287
RsaNI GTAC 4 cut(s) 124, 608, 798, 1286
RseI CAYNNNNRTG 3 cut(s) 665, 1208, 1539
SacI GAGCTC 2 cut(s) 141, 464
SacII CCGCGG 1 cut(s) 1149
Sau3AI GATC 5 cut(s) 183, 1011, 1016, 1077, 1378
Sau96I GGNCC 3 cut(s) 216, 1025, 1180
SchI GAGTC 1 cut(s) 1353
ScrFI CCNGG 1 cut(s) 284
SduI GDGCHC 5 cut(s) 141, 464, 656, 783, 887
SfaNI GCATC 5 cut(s) 838, 1058, 1330, 1381, 1649
SfcI CTRYAG 2 cut(s) 489, 562
Sfr303I CCGCGG 1 cut(s) 1149
SgrBI CCGCGG 1 cut(s) 1149
SinI GGWCC 1 cut(s) 1025
SmiMI CAYNNNNRTG 3 cut(s) 665, 1208, 1539
Sse9I AATT 3 cut(s) 806, 927, 1170
SseBI AGGCCT 1 cut(s) 594
SspMI CTAG 2 cut(s) 1064, 1220
SstI GAGCTC 2 cut(s) 141, 464
StuI AGGCCT 1 cut(s) 594
StyD4I CCNGG 1 cut(s) 282
StyI CCWWGG 2 cut(s) 872, 1470
TaaI ACNGT 6 cut(s) 667, 743, 1090, 1123, 1159, 1351
TaiI ACGT 1 cut(s) 174
TaqI TCGA 1 cut(s) 72
TasI AATT 3 cut(s) 806, 927, 1170
TatI WGTACW 1 cut(s) 607
TauI GCSGC 7 cut(s) 53, 161, 164, 246, 387, 416, 689
TfiI GAWTC 4 cut(s) 230, 615, 868, 1108
TscAI CASTG 3 cut(s) 582, 1300, 1333
TseFI GTSAC 1 cut(s) 496
TseI GCWGC 8 cut(s) 155, 509, 587, 689, 945, 1214, 1412, 1415
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 3 cut(s) 49, 979, 1450
TspGWI ACGGA 1 cut(s) 911
TspRI CASTG 3 cut(s) 582, 1300, 1333
VpaK11BI GGWCC 1 cut(s) 1025
XcmI CCANNNNNNNNNTGG 2 cut(s) 1201, 1296
XmiI GTMKAC 1 cut(s) 735
XspI CTAG 2 cut(s) 1064, 1220
Zsp2I ATGCAT 1 cut(s) 1345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.