Rroxscaffold_4G00325940

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000830
Physical Location & Seq
Forward (+)
57980230 .. 57986192
5963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325940.1

Sequence Viewer

Length: 1563 bp
ATGGCAGAGAGATTTTCTTACTTTGGCTTGGCTGGTAACCTCATCACCTACCTCACAAATGAACTCCATGAACCAATCCCAACAGCTGCCAAAAACGTCAACACTTGGATTGGTGTCTCATCTCTCCTCCCTATTCTCGGAGCCTTTATCGCTGATTCCTATCTCGGCAGATTCAACACCATCCTTGTCTCCTCCATCATATACTGCATGGCAATGAGTTTGTTAACCGTAACGGTTTCAGCTATTCCACTGCATCTACGGAAATTAATGTTCTTTGTGGCGCTTTACCTACTGTCACTAGGGCTATCAGGGCATAAACCATGTGTGCAGACGTTCGCGGCCGACCAGTTTTCTGAGGATTCGGCGGAGGAGAGAAAGGCGAAGAGCTCCTTCTTTAACTGGTGGTACTTGGGAATTGTAGTTGGTGGCTCTTCTGCCGTTTTGGTAGTTATATATATCCAGGACAATGTAAGCTGGGCAGCAGGGTTTGGAATACTCACGGGTTCCATGGTGGTGGCATTGATTCTCTTTCTGCTGGGAATCAAGAAATACCGAAGGCAAGGACCATTGGGCAGTCCCTTCACCAAGGTGGCCCAGGTATTTGTCGCCGCCCTCAGGAAGCAGCACATGGAGGTCACAACAGGTCAAGATTTAGATGAGTATTGTCAGCAACAGCCACATACTACGTTGGTGCATACCAACCAATACAGATTTTTGGACAAGGCAATGGTGATTGACCACCAGGATGCATCAAGCGAGACCACAAATCCTTGGAGATTATGTTCACTAAATCAAGTGGAACAAGTGAAGCTCCTCCTGCGCCTCATTCCCATATGGATGTGCTGCTTAATGTTTGCAGTAGTACAATCCTTTGTGCAGACCTTCTTCACCAAGCAAGGAAGCACAATGATCCGATCACTCGGATCAATTCAACTTCCCCAGGCTTCACTCCAAATCTTTGTTGGCCTCACCATTGTTGTTGCCATTCCAATCTATGACCGTGTTTTTGTCCCCATTGCTCGAAAATTCACAGGACATTCATCAGGAATTACCATCCTACAAAGAATCGGCACTGGCCTATTAATCTCCATCATCAGCATGGTTGTTGCAGCTGTGGTAGAGACCAAAAGGGTAAAGATTGTAAGAGATCACAACCTCCTGGACAATCCGAAAGCAATAGTACCAATGAGAGTATGGTGGTTAATTCCTCAGTACATGATATGTGGTTTATCTGATGCATTTGCATTTGTTGGAATGCAAGAGTTGTTCTATGATCAAATGCCAGAGGCAATGAGAAGCTTGGGAGCAGCATTTTACCTCAGTGTTGTTGGGGTTGGATTCTTCTTAAGCAATGGGATAATAACTTTGGTGCAAACAATCAGCTCAAAGGGTGGTGAAAAATGGATTACTAACAATATCAATCGATCTCACCTGGATTACTTTTATTCGGTAATCGCAGGACTGAGCGCGTTGAATTTCTGCTTGTACGTTTGGATTGCTAAAGGATTTCTATACAAGCGAATCGAAGCCAAAGAATGCAATAAGGAGATAGAACTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

520

Amino Acids

58.08

Weight (kDa)

8.99

Isoelectric Point (pI)

34.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 5 - 450 2.4e-13 Major Facilitator Superfamily
PTR2 PF00854 58 - 464 4.8e-88 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 338, 1469
AciI CCGC 3 cut(s) 338, 365, 609
AclWI GGATC 2 cut(s) 904, 931
AcoI YGGCCR 1 cut(s) 339
AcsI RAATTY 2 cut(s) 1025, 1474
AfaI GTAC 5 cut(s) 407, 864, 1182, 1214, 1487
AfiI CCNNNNNNNGG 2 cut(s) 137, 615
AflII CTTAAG 1 cut(s) 1345
AgsI TTSAA 3 cut(s) 175, 932, 1474
AjnI CCWGG 6 cut(s) 459, 594, 741, 939, 1158, 1431
AleI CACNNNNGTG 1 cut(s) 587
AloI GAACNNNNNNTCC 2 cut(s) 766, 798
AluBI AGCT 8 cut(s) 86, 242, 387, 474, 811, 1112, 1299, 1383
AluI AGCT 8 cut(s) 86, 242, 387, 474, 811, 1112, 1299, 1383
Alw21I GWGCWC 1 cut(s) 389
Alw26I GTCTC 4 cut(s) 121, 193, 752, 1115
AlwI GGATC 2 cut(s) 904, 931
AoxI GGCC 4 cut(s) 339, 591, 964, 1075
ApeKI GCWGC 6 cut(s) 86, 479, 622, 843, 1109, 1307
ApoI RAATTY 2 cut(s) 1025, 1474
ArsI GACNNNNNNTTYG 2 cut(s) 989, 1021
AseI ATTAAT 2 cut(s) 266, 1082
AspLEI GCGC 3 cut(s) 283, 822, 1469
AspS9I GGNCC 2 cut(s) 563, 592
AsuHPI GGTGA 7 cut(s) 37, 574, 742, 880, 961, 1406, 1421
AvaII GGWCC 1 cut(s) 563
AxyI CCTNAGG 1 cut(s) 614
BanII GRGCYC 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 389
BbvI GCAGC 6 cut(s) 73, 491, 634, 830, 1121, 1319
BccI CCATC 4 cut(s) 188, 203, 1061, 1097
BceAI ACGGC 1 cut(s) 422
BciT130I CCWGG 6 cut(s) 461, 596, 743, 941, 1160, 1433
BclI TGATCA 1 cut(s) 1273
BcoDI GTCTC 4 cut(s) 121, 193, 752, 1115
BfaI CTAG 1 cut(s) 299
BfoI RGCGCY 1 cut(s) 284
BfrI CTTAAG 1 cut(s) 1345
BisI GCNGC 8 cut(s) 87, 339, 480, 609, 623, 844, 1110, 1308
BlsI GCNGC 8 cut(s) 88, 340, 481, 610, 624, 845, 1111, 1309
Bme1390I CCNGG 6 cut(s) 461, 596, 743, 941, 1160, 1433
Bme18I GGWCC 1 cut(s) 563
BmgT120I GGNCC 2 cut(s) 563, 592
BmiI GGNNCC 2 cut(s) 142, 505
BmrFI CCNGG 6 cut(s) 461, 596, 743, 941, 1160, 1433
BmsI GCATC 4 cut(s) 262, 736, 758, 1225
Bsa29I ATCGAT 1 cut(s) 1423
BsaBI GATNNNNATC 1 cut(s) 159
BsaI GGTCTC 2 cut(s) 752, 1115
BsaJI CCNNGG 5 cut(s) 507, 585, 594, 770, 939
BsaXI ACNNNNNCTCC 2 cut(s) 766, 796
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 615
Bse1I ACTGG 3 cut(s) 346, 404, 1078
Bse21I CCTNAGG 1 cut(s) 614
Bse3DI GCAATG 5 cut(s) 219, 732, 1014, 1296, 1357
Bse8I GATNNNNATC 1 cut(s) 159
BseBI CCWGG 6 cut(s) 461, 596, 743, 941, 1160, 1433
BseCI ATCGAT 1 cut(s) 1423
BseDI CCNNGG 5 cut(s) 507, 585, 594, 770, 939
BseGI GGATG 4 cut(s) 180, 751, 843, 1053
BseJI GATNNNNATC 1 cut(s) 159
BseLI CCNNNNNNNGG 2 cut(s) 137, 615
BseMI GCAATG 5 cut(s) 219, 732, 1014, 1296, 1357
BseMII CTCAG 5 cut(s) 345, 628, 1223, 1333, 1454
BseNI ACTGG 3 cut(s) 346, 404, 1078
BseRI GAGGAG 4 cut(s) 116, 181, 383, 803
BseX3I CGGCCG 1 cut(s) 339
BseXI GCAGC 6 cut(s) 73, 491, 634, 830, 1121, 1319
BseYI CCCAGC 2 cut(s) 474, 535
BsgI GTGCAG 2 cut(s) 347, 896
Bsh1236I CGCG 2 cut(s) 338, 1469
Bsh1285I CGRYCG 1 cut(s) 342
BshFI GGCC 4 cut(s) 341, 593, 966, 1077
BshVI ATCGAT 1 cut(s) 1423
BsiEI CGRYCG 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 389
BslFI GGGAC 2 cut(s) 561, 995
BslI CCNNNNNNNGG 2 cut(s) 137, 615
BsmAI GTCTC 4 cut(s) 121, 193, 752, 1115
BsmFI GGGAC 2 cut(s) 561, 995
BsmI GAATGC 2 cut(s) 1260, 1541
BsnI GGCC 4 cut(s) 341, 593, 966, 1077
Bso31I GGTCTC 2 cut(s) 752, 1115
Bsp1286I GDGCHC 1 cut(s) 389
Bsp143I GATC 6 cut(s) 909, 914, 923, 1147, 1273, 1424
Bsp19I CCATGG 1 cut(s) 507
BspACI CCGC 3 cut(s) 338, 365, 609
BspANI GGCC 4 cut(s) 341, 593, 966, 1077
BspCNI CTCAG 5 cut(s) 346, 627, 1222, 1332, 1455
BspDI ATCGAT 1 cut(s) 1423
BspFNI CGCG 2 cut(s) 338, 1469
BspLI GGNNCC 2 cut(s) 142, 505
BspPI GGATC 2 cut(s) 904, 931
BspQI GCTCTTC 2 cut(s) 377, 436
BspTI CTTAAG 1 cut(s) 1345
BspTNI GGTCTC 2 cut(s) 752, 1115
BsrDI GCAATG 5 cut(s) 219, 732, 1014, 1296, 1357
BsrI ACTGG 3 cut(s) 346, 404, 1078
BssECI CCNNGG 5 cut(s) 507, 585, 594, 770, 939
BssMI GATC 6 cut(s) 909, 914, 923, 1147, 1273, 1424
BssT1I CCWWGG 3 cut(s) 507, 585, 770
Bst2UI CCWGG 6 cut(s) 461, 596, 743, 941, 1160, 1433
Bst4CI ACNGT 4 cut(s) 229, 235, 294, 1001
Bst6I CTCTTC 2 cut(s) 377, 436
BstAFI CTTAAG 1 cut(s) 1345
BstDEI CTNAG 5 cut(s) 354, 614, 1209, 1319, 1463
BstDSI CCRYGG 1 cut(s) 507
BstEII GGTNACC 1 cut(s) 35
BstF5I GGATG 4 cut(s) 180, 751, 843, 1053
BstFNI CGCG 2 cut(s) 338, 1469
BstH2I RGCGCY 1 cut(s) 284
BstHHI GCGC 3 cut(s) 283, 822, 1469
BstKTI GATC 6 cut(s) 912, 917, 926, 1150, 1276, 1427
BstMAI GTCTC 4 cut(s) 121, 193, 752, 1115
BstMBI GATC 6 cut(s) 909, 914, 923, 1147, 1273, 1424
BstMCI CGRYCG 1 cut(s) 342
BstMWI GCNNNNNNNGC 3 cut(s) 149, 310, 817
BstNI CCWGG 6 cut(s) 461, 596, 743, 941, 1160, 1433
BstPI GGTNACC 1 cut(s) 35
BstSCI CCNGG 6 cut(s) 459, 594, 741, 939, 1158, 1431
BstUI CGCG 2 cut(s) 338, 1469
BstV1I GCAGC 6 cut(s) 73, 491, 634, 830, 1121, 1319
BstXI CCANNNNNNTGG 1 cut(s) 514
BstZI CGGCCG 1 cut(s) 339
Bsu15I ATCGAT 1 cut(s) 1423
Bsu36I CCTNAGG 1 cut(s) 614
BsuRI GGCC 4 cut(s) 341, 593, 966, 1077
BsuTUI ATCGAT 1 cut(s) 1423
BtgI CCRYGG 1 cut(s) 507
BtsCI GGATG 4 cut(s) 180, 751, 843, 1053
BtsI GCAGTG 1 cut(s) 248
BtsIMutI CAGTG 3 cut(s) 248, 1071, 1327
CfoI GCGC 3 cut(s) 283, 822, 1469
Cfr13I GGNCC 2 cut(s) 563, 592
ClaI ATCGAT 1 cut(s) 1423
Csp6I GTAC 5 cut(s) 406, 863, 1181, 1213, 1486
CviAII CATG 7 cut(s) 68, 208, 321, 508, 628, 1099, 1216
CviQI GTAC 5 cut(s) 406, 863, 1181, 1213, 1486
DdeI CTNAG 5 cut(s) 354, 614, 1209, 1319, 1463
DpnI GATC 6 cut(s) 911, 916, 925, 1149, 1275, 1426
DpnII GATC 6 cut(s) 909, 914, 923, 1147, 1273, 1424
EaeI YGGCCR 1 cut(s) 339
EagI CGGCCG 1 cut(s) 339
Eam1104I CTCTTC 2 cut(s) 377, 436
EarI CTCTTC 2 cut(s) 377, 436
EciI GGCGGA 1 cut(s) 380
Ecl136II GAGCTC 1 cut(s) 387
EclXI CGGCCG 1 cut(s) 339
Eco130I CCWWGG 3 cut(s) 507, 585, 770
Eco24I GRGCYC 1 cut(s) 389
Eco31I GGTCTC 2 cut(s) 752, 1115
Eco47I GGWCC 1 cut(s) 563
Eco52I CGGCCG 1 cut(s) 339
Eco53kI GAGCTC 1 cut(s) 387
Eco81I CCTNAGG 1 cut(s) 614
Eco91I GGTNACC 1 cut(s) 35
EcoICRI GAGCTC 1 cut(s) 387
EcoO65I GGTNACC 1 cut(s) 35
EcoRII CCWGG 6 cut(s) 459, 594, 741, 939, 1158, 1431
EcoT14I CCWWGG 3 cut(s) 507, 585, 770
EcoT22I ATGCAT 2 cut(s) 751, 1240
EcoT38I GRGCYC 1 cut(s) 389
ErhI CCWWGG 3 cut(s) 507, 585, 770
FaeI CATG 7 cut(s) 71, 211, 324, 511, 631, 1102, 1219
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FaqI GGGAC 2 cut(s) 561, 995
FatI CATG 7 cut(s) 67, 207, 320, 507, 627, 1098, 1215
FauNDI CATATG 1 cut(s) 833
FbaI TGATCA 1 cut(s) 1273
Fnu4HI GCNGC 8 cut(s) 87, 339, 480, 609, 623, 844, 1110, 1308
FokI GGATG 4 cut(s) 167, 758, 850, 1040
FriOI GRGCYC 1 cut(s) 389
Fsp4HI GCNGC 8 cut(s) 87, 339, 480, 609, 623, 844, 1110, 1308
FspBI CTAG 1 cut(s) 299
GlaI GCGC 3 cut(s) 282, 821, 1468
GluI GCNGC 8 cut(s) 87, 339, 480, 609, 623, 844, 1110, 1308
GsaI CCCAGC 2 cut(s) 478, 539
HaeII RGCGCY 1 cut(s) 284
HaeIII GGCC 4 cut(s) 341, 593, 966, 1077
HhaI GCGC 3 cut(s) 283, 822, 1469
Hin1II CATG 7 cut(s) 71, 211, 324, 511, 631, 1102, 1219
Hin6I GCGC 3 cut(s) 281, 820, 1467
HinP1I GCGC 3 cut(s) 281, 820, 1467
HincII GTYRAC 2 cut(s) 100, 225
HindII GTYRAC 2 cut(s) 100, 225
HindIII AAGCTT 1 cut(s) 1297
HinfI GANTC 8 cut(s) 155, 171, 359, 523, 540, 1065, 1338, 1521
HpaI GTTAAC 1 cut(s) 225
HphI GGTGA 7 cut(s) 37, 574, 742, 880, 961, 1406, 1421
Hpy166II GTNNAC 3 cut(s) 100, 225, 785
Hpy188I TCNGA 6 cut(s) 140, 355, 914, 923, 1170, 1234
Hpy188III TCNNGA 4 cut(s) 544, 616, 647, 1044
Hpy8I GTNNAC 3 cut(s) 100, 225, 785
HpyAV CCTTC 4 cut(s) 400, 549, 589, 892
HpyCH4III ACNGT 4 cut(s) 229, 235, 294, 1001
HpyCH4IV ACGT 4 cut(s) 96, 332, 686, 1488
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 310, 817
HpyF3I CTNAG 5 cut(s) 354, 614, 1209, 1319, 1463
HpySE526I ACGT 4 cut(s) 96, 332, 686, 1488
Hsp92II CATG 7 cut(s) 71, 211, 324, 511, 631, 1102, 1219
HspAI GCGC 3 cut(s) 281, 820, 1467
Ksp22I TGATCA 1 cut(s) 1273
KspAI GTTAAC 1 cut(s) 225
Kzo9I GATC 6 cut(s) 909, 914, 923, 1147, 1273, 1424
LguI GCTCTTC 2 cut(s) 377, 436
LmnI GCTCC 4 cut(s) 140, 392, 816, 1304
Lsp1109I GCAGC 6 cut(s) 73, 491, 634, 830, 1121, 1319
LweI GCATC 4 cut(s) 262, 736, 758, 1225
MaeI CTAG 1 cut(s) 299
MaeII ACGT 4 cut(s) 96, 332, 686, 1488
MaeIII GTNAC 4 cut(s) 35, 229, 294, 634
MalI GATC 6 cut(s) 911, 916, 925, 1149, 1275, 1426
MboI GATC 6 cut(s) 909, 914, 923, 1147, 1273, 1424
MboII GAAGA 4 cut(s) 394, 423, 877, 1333
MhlI GDGCHC 1 cut(s) 389
MluCI AATT 7 cut(s) 263, 414, 927, 1025, 1047, 1203, 1474
MmeI TCCRAC 2 cut(s) 1231, 1315
Mph1103I ATGCAT 2 cut(s) 751, 1240
MseI TTAA 7 cut(s) 224, 266, 396, 848, 1082, 1202, 1346
MslI CAYNNNNRTG 6 cut(s) 212, 512, 587, 744, 836, 1097
MspA1I CMGCKG 2 cut(s) 86, 1112
MspCI CTTAAG 1 cut(s) 1345
MspR9I CCNGG 6 cut(s) 461, 596, 743, 941, 1160, 1433
Mva1269I GAATGC 2 cut(s) 1260, 1541
MvaI CCWGG 6 cut(s) 461, 596, 743, 941, 1160, 1433
MvnI CGCG 2 cut(s) 338, 1469
MwoI GCNNNNNNNGC 3 cut(s) 149, 310, 817
NcoI CCATGG 1 cut(s) 507
NdeI CATATG 1 cut(s) 833
NdeII GATC 6 cut(s) 909, 914, 923, 1147, 1273, 1424
NlaIII CATG 7 cut(s) 71, 211, 324, 511, 631, 1102, 1219
NlaIV GGNNCC 2 cut(s) 142, 505
NmeAIII GCCGAG 1 cut(s) 144
NmuCI GTSAC 2 cut(s) 294, 634
NsiI ATGCAT 2 cut(s) 751, 1240
OliI CACNNNNGTG 1 cut(s) 587
PciSI GCTCTTC 2 cut(s) 377, 436
PctI GAATGC 2 cut(s) 1260, 1541
PfeI GAWTC 8 cut(s) 155, 171, 359, 523, 540, 1065, 1338, 1521
PfoI TCCNGGA 2 cut(s) 459, 1158
PkrI GCNGC 8 cut(s) 88, 340, 481, 610, 624, 845, 1111, 1309
PshBI ATTAAT 2 cut(s) 266, 1082
Psp124BI GAGCTC 1 cut(s) 389
Psp6I CCWGG 6 cut(s) 459, 594, 741, 939, 1158, 1431
PspEI GGTNACC 1 cut(s) 35
PspFI CCCAGC 2 cut(s) 474, 535
PspGI CCWGG 6 cut(s) 459, 594, 741, 939, 1158, 1431
PspN4I GGNNCC 2 cut(s) 142, 505
PspPI GGNCC 2 cut(s) 563, 592
PvuII CAGCTG 2 cut(s) 86, 1112
RsaI GTAC 5 cut(s) 407, 864, 1182, 1214, 1487
RsaNI GTAC 5 cut(s) 406, 863, 1181, 1213, 1486
RseI CAYNNNNRTG 6 cut(s) 212, 512, 587, 744, 836, 1097
SacI GAGCTC 1 cut(s) 389
SapI GCTCTTC 2 cut(s) 377, 436
SaqAI TTAA 7 cut(s) 224, 266, 396, 848, 1082, 1202, 1346
SatI GCNGC 8 cut(s) 87, 339, 480, 609, 623, 844, 1110, 1308
Sau3AI GATC 6 cut(s) 909, 914, 923, 1147, 1273, 1424
Sau96I GGNCC 2 cut(s) 563, 592
ScrFI CCNGG 6 cut(s) 461, 596, 743, 941, 1160, 1433
SduI GDGCHC 1 cut(s) 389
SfaNI GCATC 4 cut(s) 262, 736, 758, 1225
SinI GGWCC 1 cut(s) 563
SmiMI CAYNNNNRTG 6 cut(s) 212, 512, 587, 744, 836, 1097
SmlI CTYRAG 1 cut(s) 1345
SmoI CTYRAG 1 cut(s) 1345
Sse9I AATT 7 cut(s) 263, 414, 927, 1025, 1047, 1203, 1474
SsiI CCGC 3 cut(s) 338, 365, 609
SspMI CTAG 1 cut(s) 299
SstI GAGCTC 1 cut(s) 389
StyD4I CCNGG 6 cut(s) 459, 594, 741, 939, 1158, 1431
StyI CCWWGG 3 cut(s) 507, 585, 770
TaaI ACNGT 4 cut(s) 229, 235, 294, 1001
TaiI ACGT 4 cut(s) 99, 335, 689, 1491
TaqI TCGA 3 cut(s) 1021, 1423, 1524
TasI AATT 7 cut(s) 263, 414, 927, 1025, 1047, 1203, 1474
TatI WGTACW 2 cut(s) 862, 1212
TauI GCSGC 2 cut(s) 341, 611
TfiI GAWTC 8 cut(s) 155, 171, 359, 523, 540, 1065, 1338, 1521
Tru1I TTAA 7 cut(s) 224, 266, 396, 848, 1082, 1202, 1346
Tru9I TTAA 7 cut(s) 224, 266, 396, 848, 1082, 1202, 1346
TscAI CASTG 3 cut(s) 255, 1078, 1327
TseFI GTSAC 2 cut(s) 294, 634
TseI GCWGC 6 cut(s) 86, 479, 622, 843, 1109, 1307
Tsp45I GTSAC 2 cut(s) 294, 634
TspDTI ATGAA 3 cut(s) 75, 84, 1029
TspGWI ACGGA 1 cut(s) 274
TspRI CASTG 3 cut(s) 255, 1078, 1327
Vha464I CTTAAG 1 cut(s) 1345
VpaK11BI GGWCC 1 cut(s) 563
VspI ATTAAT 2 cut(s) 266, 1082
XapI RAATTY 2 cut(s) 1025, 1474
XcmI CCANNNNNNNNNTGG 3 cut(s) 959, 1096, 1191
XspI CTAG 1 cut(s) 299
Zsp2I ATGCAT 2 cut(s) 751, 1240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.