FvH4_7g03030

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000830
Physical Location & Seq
Reverse (-)
3388539 .. 3399273
10735 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g03030.t1

Sequence Viewer

Length: 1656 bp
ATGCTCTCTCCCCAAAGGACCCTCTCTCGTGGTGGCTGGAACGCCGCCATTTTCATCATCTTCGTGGAAGTTGCAGAGAGGTTTGCTTACTATGGCCTGGCTGGTAATCTCATCATGTACCTCACCAATGAGCTCCACCAGCCCACCGCCACCGCAGCCAAGAACGTCAACATGTGGATCGGTCTCTCCTCCCTCTTCCCCATTCTCGGTGCCATCGTCGCCGATTCCTACCTCGGTCGCTTCAAGACAATCATCTATTCCACCTGCATTTATTTCCTGGGGATGGTTATGCTGTGCCTATCGGCTTCGGTGGTTCCTCGGCATTACCGTGAAGGGGCATACTTCGTGGCGCTTTATATTTTGGCCGTGGGAGAAGGCGGCCACAAGCCTTGCGTGCAAACCTTCGCTGCCGACCAGTTTGATGAAGAGACTGATGAGGACAAGGAGGTCAAGAGCTCATTCTTCAACTGGTGGTATTTGGCTATAGTTTTCACCGCTATTGTTGCCACACTTGGTATCATCTATATTCAGGATGATGTGAGCTGGGTGCTAAGCTATGGTGTCTTGGCGGTGATAATAGCGGTAGGGCTGGTTTTATTTCTTTTAGGAATGCAAAGGTATCGGTACCAAGATAATCTGCTTGTTGGGGCTAGTCCCTTGACGATGGTGGCACAAGTGTTTGTGGCAGCAGCTCGGAAGTGGCGCGTGAAGCTTGGCCATCGGAATGATGTTGTCTACTACGGTGACGATGAGAGTGGGAGCAGTGGGGTTGATTCCAAGCACAAGACATTGGCGCGTACTAAACAGTTCAGACTTTTGGACAAGGCAATGATCGTTGATGAACATGATGCATCAAGCAAAATGAGAAATCCTTGGAGGTTGTGCTCTCTGAATCATGTGGAAGAAGTGAAGCTTGTTGTCCGCCTAATTCCCATATGGTTGAGCTGCCTAATGTTTCACGCAGTTCAATCTCAACTCCACACCTACTTCATCAAACAAGGTTCTACGATGATCAGATCTATTGGACCCAAGTTCAGGTTCCCCCCTGCATCACTTCAAGCCGTAGTTGGCATCATGATCGTAACTACCGTCCTAATTTACGACCGAATTATTGTTCCGACAGCACGAAAGGTCACAGGACATCCCGCAGGCATAACGGTACTGCAACGAATTGGTGTTGGCCTATTCTTGTCCGTAATCAACATGGTTGTGGCAGCTCTAGTGGAAGCTAAAAGGGTTGGTGTTGCTAGAGATAATAACCTCATGGACAACCCGAAAGCAATAGTACCAATTAGTGTCTGGTGGTTACTCCCACAATTCATTGTCACAGGTTTGTCGGATGCGTTCACCGTTGTAGGACTCCAAGAGTTGTTCTATGATCAAATGCCAGATGCAATGAGAAGCTTGGGAGCTGCAGCATACATCAGTATTCTTGGAGTTGGAAGCTTTCTCAGTAGCTTCATCATATCTTTGGTGGAAACTTTTAGCAGAAACTCTGGTGAGCCATGGTTAGGTAACAACCTTAATCGCGCTCACCTTGATGACTTCTACTGGGTGCTTGCGGGACTAAGTGCTTTATTTTTTGGTGTTTATATATGGGTTGCAAACCGATTTGTTTACAAGAGAGTTGTAGGGGAAGCATCAAATGAGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

552

Amino Acids

61.44

Weight (kDa)

8.75

Isoelectric Point (pI)

27.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 81 - 497 8.9e-86 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 272
AasI GACNNNNNNGTC 1 cut(s) 446
Acc36I ACCTGC 1 cut(s) 272
Acc65I GGTACC 1 cut(s) 624
AccB1I GGYRCC 2 cut(s) 209, 624
AccI GTMKAC 1 cut(s) 735
AccII CGCG 3 cut(s) 705, 796, 1530
AclWI GGATC 1 cut(s) 185
AcoI YGGCCR 3 cut(s) 363, 379, 715
AfaI GTAC 5 cut(s) 119, 626, 799, 1161, 1287
AfiI CCNNNNNNNGG 5 cut(s) 206, 283, 334, 1035, 1511
AflIII ACRYGT 1 cut(s) 171
AgsI TTSAA 4 cut(s) 244, 466, 968, 1058
AjnI CCWGG 2 cut(s) 96, 276
AjuI GAANNNNNNNTTGG 2 cut(s) 1022, 1054
Alw21I GWGCWC 3 cut(s) 135, 458, 887
Alw26I GTCTC 2 cut(s) 188, 422
AlwI GGATC 1 cut(s) 185
AoxI GGCC 5 cut(s) 94, 363, 379, 715, 1180
ApeKI GCWGC 8 cut(s) 155, 407, 686, 689, 945, 1214, 1412, 1415
Asp718I GGTACC 1 cut(s) 624
AspLEI GCGC 4 cut(s) 352, 705, 796, 1532
AspS9I GGNCC 2 cut(s) 18, 1025
AsuHPI GGTGA 7 cut(s) 115, 484, 583, 755, 1339, 1511, 1526
AvaII GGWCC 2 cut(s) 18, 1025
BalI TGGCCA 1 cut(s) 717
BanI GGYRCC 2 cut(s) 209, 624
BanII GRGCYC 2 cut(s) 135, 458
BauI CACGAG 1 cut(s) 27
Bbv12I GWGCWC 3 cut(s) 135, 458, 887
BbvI GCAGC 8 cut(s) 167, 394, 698, 701, 932, 1226, 1399, 1427
BccI CCATC 4 cut(s) 221, 277, 658, 726
BceAI ACGGC 2 cut(s) 350, 1046
BcgI CGANNNNNNTGC 4 cut(s) 602, 636, 1060, 1094
BciT130I CCWGG 2 cut(s) 98, 278
BclI TGATCA 2 cut(s) 1011, 1378
BcoDI GTCTC 2 cut(s) 188, 422
BfaI CTAG 3 cut(s) 651, 1220, 1248
BfmI CTRYAG 2 cut(s) 483, 1413
BfoI RGCGCY 1 cut(s) 353
BfuAI ACCTGC 1 cut(s) 272
BglII AGATCT 1 cut(s) 1016
BlpI GCTNAGC 1 cut(s) 551
Bme1390I CCNGG 2 cut(s) 98, 278
Bme18I GGWCC 2 cut(s) 18, 1025
BmgT120I GGNCC 2 cut(s) 18, 1025
BmiI GGNNCC 6 cut(s) 20, 211, 315, 626, 1027, 1040
BmrFI CCNGG 2 cut(s) 98, 278
BmrI ACTGGG 1 cut(s) 1561
BmsI GCATC 7 cut(s) 838, 860, 1058, 1080, 1330, 1381, 1649
BmuI ACTGGG 1 cut(s) 1561
Bpu1102I GCTNAGC 1 cut(s) 551
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 6 cut(s) 232, 277, 317, 366, 872, 1505
Bsc4I CCNNNNNNNGG 5 cut(s) 206, 283, 334, 1035, 1511
Bse1I ACTGG 3 cut(s) 415, 473, 1556
Bse3DI GCAATG 2 cut(s) 834, 1401
BseBI CCWGG 2 cut(s) 98, 278
BseDI CCNNGG 6 cut(s) 232, 277, 317, 366, 872, 1505
BseGI GGATG 4 cut(s) 288, 538, 1141, 1345
BseLI CCNNNNNNNGG 5 cut(s) 206, 283, 334, 1035, 1511
BseMI GCAATG 2 cut(s) 834, 1401
BseMII CTCAG 1 cut(s) 1465
BseNI ACTGG 3 cut(s) 415, 473, 1556
BseRI GAGGAG 1 cut(s) 178
BseXI GCAGC 8 cut(s) 167, 394, 698, 701, 932, 1226, 1399, 1427
BseYI CCCAGC 1 cut(s) 543
Bsh1236I CGCG 3 cut(s) 705, 796, 1530
Bsh1285I CGRYCG 2 cut(s) 238, 1105
BshFI GGCC 5 cut(s) 96, 365, 381, 717, 1182
BshNI GGYRCC 2 cut(s) 209, 624
BsiEI CGRYCG 2 cut(s) 238, 1105
BsiHKAI GWGCWC 3 cut(s) 135, 458, 887
BslFI GGGAC 2 cut(s) 639, 1578
BslI CCNNNNNNNGG 5 cut(s) 206, 283, 334, 1035, 1511
BsmAI GTCTC 2 cut(s) 188, 422
BsmFI GGGAC 2 cut(s) 639, 1578
BsmI GAATGC 1 cut(s) 615
BsnI GGCC 5 cut(s) 96, 365, 381, 717, 1182
Bso31I GGTCTC 1 cut(s) 188
Bsp1286I GDGCHC 3 cut(s) 135, 458, 887
Bsp143I GATC 6 cut(s) 177, 831, 1011, 1016, 1077, 1378
Bsp1720I GCTNAGC 1 cut(s) 551
Bsp19I CCATGG 1 cut(s) 1505
BspANI GGCC 5 cut(s) 96, 365, 381, 717, 1182
BspCNI CTCAG 1 cut(s) 1464
BspFNI CGCG 3 cut(s) 705, 796, 1530
BspHI TCATGA 1 cut(s) 1074
BspLI GGNNCC 6 cut(s) 20, 211, 315, 626, 1027, 1040
BspMAI CTGCAG 1 cut(s) 1417
BspMI ACCTGC 1 cut(s) 272
BspPI GGATC 1 cut(s) 185
BspT107I GGYRCC 2 cut(s) 209, 624
BspTNI GGTCTC 1 cut(s) 188
BsrDI GCAATG 2 cut(s) 834, 1401
BsrI ACTGG 3 cut(s) 415, 473, 1556
BssECI CCNNGG 6 cut(s) 232, 277, 317, 366, 872, 1505
BssMI GATC 6 cut(s) 177, 831, 1011, 1016, 1077, 1378
BssSI CACGAG 1 cut(s) 27
BssT1I CCWWGG 2 cut(s) 872, 1505
Bst2BI CACGAG 1 cut(s) 27
Bst2UI CCWGG 2 cut(s) 98, 278
Bst4CI ACNGT 6 cut(s) 329, 743, 807, 1090, 1159, 1351
Bst6I CTCTTC 2 cut(s) 200, 420
BstC8I GCNNGC 3 cut(s) 395, 1150, 1560
BstDEI CTNAG 3 cut(s) 551, 1451, 1568
BstDSI CCRYGG 2 cut(s) 366, 1505
BstF5I GGATG 4 cut(s) 288, 538, 1141, 1345
BstFNI CGCG 3 cut(s) 705, 796, 1530
BstH2I RGCGCY 1 cut(s) 353
BstHHI GCGC 4 cut(s) 352, 705, 796, 1532
BstKTI GATC 6 cut(s) 180, 834, 1014, 1019, 1080, 1381
BstMAI GTCTC 2 cut(s) 188, 422
BstMBI GATC 6 cut(s) 177, 831, 1011, 1016, 1077, 1378
BstMCI CGRYCG 2 cut(s) 238, 1105
BstMWI GCNNNNNNNGC 6 cut(s) 139, 155, 218, 394, 503, 709
BstNI CCWGG 2 cut(s) 98, 278
BstNSI RCATGY 1 cut(s) 175
BstSCI CCNGG 2 cut(s) 96, 276
BstSFI CTRYAG 2 cut(s) 483, 1413
BstUI CGCG 3 cut(s) 705, 796, 1530
BstV1I GCAGC 8 cut(s) 167, 394, 698, 701, 932, 1226, 1399, 1427
BstX2I RGATCY 1 cut(s) 1016
BstYI RGATCY 1 cut(s) 1016
BsuRI GGCC 5 cut(s) 96, 365, 381, 717, 1182
BtgI CCRYGG 2 cut(s) 366, 1505
BtsCI GGATG 4 cut(s) 288, 538, 1141, 1345
BtsI GCAGTG 1 cut(s) 769
BtsIMutI CAGTG 1 cut(s) 769
BveI ACCTGC 1 cut(s) 272
Cac8I GCNNGC 3 cut(s) 395, 1150, 1560
CciI TCATGA 1 cut(s) 1074
CfoI GCGC 4 cut(s) 352, 705, 796, 1532
Cfr13I GGNCC 2 cut(s) 18, 1025
Csp6I GTAC 5 cut(s) 118, 625, 798, 1160, 1286
CspCI CAANNNNNGTGG 2 cut(s) 371, 406
CviAII CATG 8 cut(s) 115, 172, 845, 896, 1075, 1204, 1264, 1506
CviQI GTAC 5 cut(s) 118, 625, 798, 1160, 1286
DdeI CTNAG 3 cut(s) 551, 1451, 1568
DpnI GATC 6 cut(s) 179, 833, 1013, 1018, 1079, 1380
DpnII GATC 6 cut(s) 177, 831, 1011, 1016, 1077, 1378
DrdI GACNNNNNNGTC 1 cut(s) 446
DseDI GACNNNNNNGTC 1 cut(s) 446
EaeI YGGCCR 3 cut(s) 363, 379, 715
Eam1104I CTCTTC 2 cut(s) 200, 420
EarI CTCTTC 2 cut(s) 200, 420
EciI GGCGGA 1 cut(s) 911
Ecl136II GAGCTC 2 cut(s) 133, 456
Eco130I CCWWGG 2 cut(s) 872, 1505
Eco24I GRGCYC 2 cut(s) 135, 458
Eco31I GGTCTC 1 cut(s) 188
Eco47I GGWCC 2 cut(s) 18, 1025
Eco53kI GAGCTC 2 cut(s) 133, 456
EcoICRI GAGCTC 2 cut(s) 133, 456
EcoO109I RGGNCCY 1 cut(s) 18
EcoRII CCWGG 2 cut(s) 96, 276
EcoT14I CCWWGG 2 cut(s) 872, 1505
EcoT22I ATGCAT 1 cut(s) 853
EcoT38I GRGCYC 2 cut(s) 135, 458
ErhI CCWWGG 2 cut(s) 872, 1505
FaeI CATG 8 cut(s) 118, 175, 848, 899, 1078, 1207, 1267, 1509
FalI AAGNNNNNCTT 2 cut(s) 897, 929
FaqI GGGAC 2 cut(s) 639, 1578
FatI CATG 8 cut(s) 114, 171, 844, 895, 1074, 1203, 1263, 1505
FauI CCCGC 2 cut(s) 1153, 1555
FauNDI CATATG 1 cut(s) 935
FbaI TGATCA 2 cut(s) 1011, 1378
FblI GTMKAC 1 cut(s) 735
FokI GGATG 4 cut(s) 295, 545, 1128, 1352
FriOI GRGCYC 2 cut(s) 135, 458
FspBI CTAG 3 cut(s) 651, 1220, 1248
GlaI GCGC 4 cut(s) 351, 704, 795, 1531
GsaI CCCAGC 1 cut(s) 547
HaeII RGCGCY 1 cut(s) 353
HaeIII GGCC 5 cut(s) 96, 365, 381, 717, 1182
HhaI GCGC 4 cut(s) 352, 705, 796, 1532
Hin1II CATG 8 cut(s) 118, 175, 848, 899, 1078, 1207, 1267, 1509
Hin6I GCGC 4 cut(s) 350, 703, 794, 1530
HinP1I GCGC 4 cut(s) 350, 703, 794, 1530
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 4 cut(s) 710, 911, 1402, 1444
HinfI GANTC 4 cut(s) 224, 773, 892, 1359
HphI GGTGA 7 cut(s) 115, 484, 583, 755, 1339, 1511, 1526
Hpy166II GTNNAC 4 cut(s) 169, 736, 1347, 1618
Hpy188I TCNGA 7 cut(s) 696, 723, 812, 891, 1016, 1119, 1339
Hpy188III TCNNGA 4 cut(s) 244, 451, 530, 1075
Hpy8I GTNNAC 4 cut(s) 169, 736, 1347, 1618
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 3 cut(s) 326, 368, 412
HpyCH4III ACNGT 6 cut(s) 329, 743, 807, 1090, 1159, 1351
HpyCH4IV ACGT 1 cut(s) 165
HpyF10VI GCNNNNNNNGC 6 cut(s) 139, 155, 218, 394, 503, 709
HpyF3I CTNAG 3 cut(s) 551, 1451, 1568
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 8 cut(s) 118, 175, 848, 899, 1078, 1207, 1267, 1509
HspAI GCGC 4 cut(s) 350, 703, 794, 1530
KpnI GGTACC 1 cut(s) 628
Ksp22I TGATCA 2 cut(s) 1011, 1378
Kzo9I GATC 6 cut(s) 177, 831, 1011, 1016, 1077, 1378
LmnI GCTCC 3 cut(s) 138, 759, 1409
Lsp1109I GCAGC 8 cut(s) 167, 394, 698, 701, 932, 1226, 1399, 1427
LweI GCATC 7 cut(s) 838, 860, 1058, 1080, 1330, 1381, 1649
MaeI CTAG 3 cut(s) 651, 1220, 1248
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 6 cut(s) 743, 1081, 1132, 1305, 1324, 1514
MalI GATC 6 cut(s) 179, 833, 1013, 1018, 1079, 1380
MboI GATC 6 cut(s) 177, 831, 1011, 1016, 1077, 1378
MboII GAAGA 5 cut(s) 52, 187, 437, 454, 914
MflI RGATCY 1 cut(s) 1016
MhlI GDGCHC 3 cut(s) 135, 458, 887
MlsI TGGCCA 1 cut(s) 717
MluCI AATT 6 cut(s) 927, 1095, 1107, 1170, 1290, 1316
MluNI TGGCCA 1 cut(s) 717
MlyI GAGTC 1 cut(s) 1353
MmeI TCCRAC 3 cut(s) 1142, 1317, 1420
Mox20I TGGCCA 1 cut(s) 717
Mph1103I ATGCAT 1 cut(s) 853
MscI TGGCCA 1 cut(s) 717
MseI TTAA 1 cut(s) 1524
MslI CAYNNNNRTG 5 cut(s) 62, 327, 723, 1208, 1539
Msp20I TGGCCA 1 cut(s) 717
MspR9I CCNGG 2 cut(s) 98, 278
Mva1269I GAATGC 1 cut(s) 615
MvaI CCWGG 2 cut(s) 98, 278
MvnI CGCG 3 cut(s) 705, 796, 1530
MwoI GCNNNNNNNGC 6 cut(s) 139, 155, 218, 394, 503, 709
NcoI CCATGG 1 cut(s) 1505
NdeI CATATG 1 cut(s) 935
NdeII GATC 6 cut(s) 177, 831, 1011, 1016, 1077, 1378
NlaIII CATG 8 cut(s) 118, 175, 848, 899, 1078, 1207, 1267, 1509
NlaIV GGNNCC 6 cut(s) 20, 211, 315, 626, 1027, 1040
NmeAIII GCCGAG 1 cut(s) 298
NmuCI GTSAC 3 cut(s) 743, 1132, 1324
NsiI ATGCAT 1 cut(s) 853
NspI RCATGY 1 cut(s) 175
PagI TCATGA 1 cut(s) 1074
PaqCI CACCTGC 1 cut(s) 272
PciI ACATGT 1 cut(s) 171
PcsI WCGNNNNNNNCGW 3 cut(s) 213, 325, 1086
PctI GAATGC 1 cut(s) 615
PfeI GAWTC 3 cut(s) 224, 773, 892
PleI GAGTC 1 cut(s) 1353
PpsI GAGTC 1 cut(s) 1353
PpuMI RGGWCCY 1 cut(s) 18
PscI ACATGT 1 cut(s) 171
Psp124BI GAGCTC 2 cut(s) 135, 458
Psp5II RGGWCCY 1 cut(s) 18
Psp6I CCWGG 2 cut(s) 96, 276
PspFI CCCAGC 1 cut(s) 543
PspGI CCWGG 2 cut(s) 96, 276
PspN4I GGNNCC 6 cut(s) 20, 211, 315, 626, 1027, 1040
PspPI GGNCC 2 cut(s) 18, 1025
PspPPI RGGWCCY 1 cut(s) 18
PstI CTGCAG 1 cut(s) 1417
PsuI RGATCY 1 cut(s) 1016
RsaI GTAC 5 cut(s) 119, 626, 799, 1161, 1287
RsaNI GTAC 5 cut(s) 118, 625, 798, 1160, 1286
RseI CAYNNNNRTG 5 cut(s) 62, 327, 723, 1208, 1539
SacI GAGCTC 2 cut(s) 135, 458
SaqAI TTAA 1 cut(s) 1524
Sau3AI GATC 6 cut(s) 177, 831, 1011, 1016, 1077, 1378
Sau96I GGNCC 2 cut(s) 18, 1025
SchI GAGTC 1 cut(s) 1353
ScrFI CCNGG 2 cut(s) 98, 278
SduI GDGCHC 3 cut(s) 135, 458, 887
SfaNI GCATC 7 cut(s) 838, 860, 1058, 1080, 1330, 1381, 1649
SfcI CTRYAG 2 cut(s) 483, 1413
SinI GGWCC 2 cut(s) 18, 1025
SmiMI CAYNNNNRTG 5 cut(s) 62, 327, 723, 1208, 1539
Sse9I AATT 6 cut(s) 927, 1095, 1107, 1170, 1290, 1316
SspMI CTAG 3 cut(s) 651, 1220, 1248
SstI GAGCTC 2 cut(s) 135, 458
StyD4I CCNGG 2 cut(s) 96, 276
StyI CCWWGG 2 cut(s) 872, 1505
TaaI ACNGT 6 cut(s) 329, 743, 807, 1090, 1159, 1351
TaiI ACGT 1 cut(s) 168
TaqII GACCGA 3 cut(s) 170, 224, 1119
TasI AATT 6 cut(s) 927, 1095, 1107, 1170, 1290, 1316
TauI GCSGC 2 cut(s) 47, 381
TfiI GAWTC 3 cut(s) 224, 773, 892
Tru1I TTAA 1 cut(s) 1524
Tru9I TTAA 1 cut(s) 1524
TscAI CASTG 1 cut(s) 769
TseFI GTSAC 3 cut(s) 743, 1132, 1324
TseI GCWGC 8 cut(s) 155, 407, 686, 689, 945, 1214, 1412, 1415
Tsp45I GTSAC 3 cut(s) 743, 1132, 1324
TspDTI ATGAA 6 cut(s) 43, 438, 855, 979, 1309, 1450
TspGWI ACGGA 1 cut(s) 1183
TspRI CASTG 1 cut(s) 769
VpaK11BI GGWCC 2 cut(s) 18, 1025
XceI RCATGY 1 cut(s) 175
XcmI CCANNNNNNNNNTGG 1 cut(s) 1296
XmiI GTMKAC 1 cut(s) 735
XspI CTAG 3 cut(s) 651, 1220, 1248
Zsp2I ATGCAT 1 cut(s) 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.