Rroxscaffold_4G00325880

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000830
Physical Location & Seq
Forward (+)
57886679 .. 57892099
5421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325880.1

Sequence Viewer

Length: 1611 bp
ATGACACTCGGAAACAACGGGAGTGAGTTACTCATCAAGATGCTCTCCTCCGACCACCAAAAGACCCCCTCTCGTGGTGGCTGGAACGCCGCCATTTTCATCATCTTCGTCGAGGTTGCAGAGAGGTTTGCTTACTATGGCCTCATAGGTAACCTTATCATGTACCTCACCAATGAGCTCCACCAGCCCACCGCTGCCGCCGCCAAGAACGTCAACTTGTGGATCGGCCTCTCCTCCCTCTTCCCCGTCCTCGGGGCCATCGTCGCCGATTCTTACCTCGGCCGCTTCAAGACAATCATCTACTCCACCTGCATTTATTTTGTGGGAATGGTTATGCTGTGCTTATCTGCTTCGGTGGTTCCTCGGCATTATCGTGAAGGGGCATACTTCGTGGGGCTTTACATTCTGGCGGTGGGAGAAGGCGGCCACAAGCCTTGCGTGCAGACCTTCGCCGCCGACCAGTTTGAGGAGGAGACTGATGAAGACAAGGAGGCTAAGAGCTCATTCTTCAACTGGTGGTATTTGGCTATAGTTGTCACCGCTACTGCTGCCACACTTGGTATCATCTATATTCAGGATAATGTGAGCTGGGTGCTGAGCTATAGTGTCTTGGCAGTGGTAATAGCAGCAGGCCTCCTATTATTTGTACTCGGAATCCGAAGGTATCGGAACGACGATAATATGTTGGGGAGCCCCTTCACGACGGTGGCACAAGTATTTGTGGCGGCAGCTTGGAAGTGGCGCGTAAAGACGGGTCGTCGGAATGATGTTGTCTACTACGGTGATGATGAGGGTGAGAGCAGTGGGGTTCACTCCAAGCACAAGCCACTGGCTCGTACTAAACAATTCAGATTTCTGGACAAGGCAATGATTATTGACGAACATGATGCGTCAAGCAAAATGAGGAATCCTTGGAGTTCAATCTCAACTCCACACCTACTTCATCAAACAAGGTTCCACGATGATCAGATCCATCGGACCCAACTTTCGGTTCCCCCTGCATCACTTCAAGCCCTAGTTGGGATTGTGATCGTAACTACCGTTCCAATCTACGACCGGATTCTTGTCCCGACAGTAAGAAAGTTCACAGGACATCCCGCGGGCATAACAGTCCTCCAACGAATTGGTGTGGGCCTATTCTTGTCCATAATCAACATGGTTGTGGCAGCTCTAGTGGAAGCTAAAAGGGTTGGTGTTGCAAGAGAATATAACCTCATGGATAACCCAAAAGCAATGGTACCAATCAGTGTCTGGTGGTTACTCCCACAATACTTTATCACTGGTTTGTCTGATGCATTCACCGTTATAGGACTCCAAGAGTTGTTCTATGATCAAATGCCAGATGCAATGAGAAGCTTGGGAGCTGCAGCATACATTAGTATTCTTGGAGTTGGAAGCTTTCTTAGCAGTTTCATCATATCCTTGGTGGAAACTATTACCAGAAACTCTGGTGAGCCGTGGTTAGGTAACAATCTCAACCGCGCACACCTTGATGACTTCTACTGGGTGCTTGCAGGACTAAGCGCTCTATTTTTCGGTGTTTATGTGTGGGTTGCAAAGCGATTTGTTTACAAGAAAGTTGTAGGGGAAGCATCAAGCGAGGAGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

536

Amino Acids

59.64

Weight (kDa)

7.23

Isoelectric Point (pI)

30.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 39 - 469 5e-11 Major Facilitator Superfamily
PTR2 PF00854 96 - 306 3.2e-37 POT family
PTR2 PF00854 315 - 481 7.7e-33 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 317
Acc36I ACCTGC 1 cut(s) 317
Acc65I GGTACC 1 cut(s) 1237
AccB1I GGYRCC 1 cut(s) 1237
AccI GTMKAC 1 cut(s) 774
AccII CGCG 3 cut(s) 744, 1100, 1482
AclWI GGATC 2 cut(s) 230, 964
AcoI YGGCCR 2 cut(s) 280, 424
AfaI GTAC 4 cut(s) 164, 648, 838, 1239
AfeI AGCGCT 1 cut(s) 1525
AfiI CCNNNNNNNGG 7 cut(s) 74, 251, 252, 466, 988, 1020, 1463
AgsI TTSAA 4 cut(s) 289, 511, 921, 1010
AhdI GACNNNNNGTC 1 cut(s) 756
AjuI GAANNNNNNNTTGG 2 cut(s) 975, 1007
AleI CACNNNNGTG 1 cut(s) 704
Alw21I GWGCWC 2 cut(s) 180, 503
Alw26I GTCTC 1 cut(s) 467
AlwI GGATC 2 cut(s) 230, 964
AlwNI CAGNNNCTG 1 cut(s) 1251
Ama87I CYCGRG 1 cut(s) 251
Aor51HI AGCGCT 1 cut(s) 1525
AoxI GGCC 7 cut(s) 139, 226, 255, 280, 424, 631, 1132
ApeKI GCWGC 7 cut(s) 194, 548, 626, 728, 1166, 1364, 1367
ArsI GACNNNNNNTTYG 2 cut(s) 970, 1002
Asp718I GGTACC 1 cut(s) 1237
AspLEI GCGC 3 cut(s) 744, 1484, 1526
AspS9I GGNCC 3 cut(s) 255, 978, 1132
AsuHPI GGTGA 6 cut(s) 160, 529, 794, 806, 1291, 1463
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 1 cut(s) 978
BanI GGYRCC 1 cut(s) 1237
BanII GRGCYC 3 cut(s) 180, 503, 695
BauI CACGAG 1 cut(s) 72
BbsI GAAGAC 1 cut(s) 489
Bbv12I GWGCWC 2 cut(s) 180, 503
BbvI GCAGC 7 cut(s) 181, 535, 638, 740, 1178, 1351, 1379
BccI CCATC 2 cut(s) 266, 981
BceAI ACGGC 1 cut(s) 1441
BcgI CGANNNNNNTGC 2 cut(s) 869, 903
BclI TGATCA 2 cut(s) 964, 1330
BcoDI GTCTC 1 cut(s) 467
BfaI CTAG 2 cut(s) 1016, 1172
BfmI CTRYAG 3 cut(s) 528, 601, 1365
BfoI RGCGCY 1 cut(s) 1527
BfuAI ACCTGC 1 cut(s) 317
BlpI GCTNAGC 1 cut(s) 596
Bme18I GGWCC 1 cut(s) 978
BmeRI GACNNNNNGTC 1 cut(s) 756
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 3 cut(s) 255, 978, 1132
BmiI GGNNCC 7 cut(s) 256, 360, 692, 956, 980, 993, 1239
BmrI ACTGGG 1 cut(s) 1513
BmsI GCATC 6 cut(s) 30, 877, 1010, 1282, 1333, 1601
BmuI ACTGGG 1 cut(s) 1513
BpiI GAAGAC 1 cut(s) 489
Bpu1102I GCTNAGC 1 cut(s) 596
BsaBI GATNNNNATC 1 cut(s) 1028
BsaJI CCNNGG 7 cut(s) 250, 277, 362, 911, 1098, 1422, 1457
BsaWI WCCGGW 1 cut(s) 1056
Bsc4I CCNNNNNNNGG 7 cut(s) 74, 251, 252, 466, 988, 1020, 1463
Bse1I ACTGG 5 cut(s) 460, 518, 834, 1285, 1508
Bse3DI GCAATG 3 cut(s) 873, 1239, 1353
Bse8I GATNNNNATC 1 cut(s) 1028
BseDI CCNNGG 7 cut(s) 250, 277, 362, 911, 1098, 1422, 1457
BseGI GGATG 1 cut(s) 1093
BseJI GATNNNNATC 1 cut(s) 1028
BseLI CCNNNNNNNGG 7 cut(s) 74, 251, 252, 466, 988, 1020, 1463
BseMI GCAATG 3 cut(s) 873, 1239, 1353
BseMII CTCAG 1 cut(s) 587
BseNI ACTGG 5 cut(s) 460, 518, 834, 1285, 1508
BseRI GAGGAG 4 cut(s) 37, 223, 482, 485
BseX3I CGGCCG 1 cut(s) 280
BseXI GCAGC 7 cut(s) 181, 535, 638, 740, 1178, 1351, 1379
BseYI CCCAGC 1 cut(s) 588
BsgI GTGCAG 1 cut(s) 461
Bsh1236I CGCG 3 cut(s) 744, 1100, 1482
Bsh1285I CGRYCG 2 cut(s) 283, 1057
BshFI GGCC 7 cut(s) 141, 228, 257, 282, 426, 633, 1134
BshNI GGYRCC 1 cut(s) 1237
BsiEI CGRYCG 2 cut(s) 283, 1057
BsiHKAI GWGCWC 2 cut(s) 180, 503
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 1 cut(s) 1057
BslFI GGGAC 1 cut(s) 1052
BslI CCNNNNNNNGG 7 cut(s) 74, 251, 252, 466, 988, 1020, 1463
BsmAI GTCTC 1 cut(s) 467
BsmFI GGGAC 1 cut(s) 1052
BsmI GAATGC 1 cut(s) 1295
BsnI GGCC 7 cut(s) 141, 228, 257, 282, 426, 633, 1134
BsoBI CYCGRG 1 cut(s) 251
Bsp1286I GDGCHC 3 cut(s) 180, 503, 695
Bsp143I GATC 5 cut(s) 222, 964, 969, 1029, 1330
Bsp1720I GCTNAGC 1 cut(s) 596
BspANI GGCC 7 cut(s) 141, 228, 257, 282, 426, 633, 1134
BspCNI CTCAG 1 cut(s) 588
BspFNI CGCG 3 cut(s) 744, 1100, 1482
BspLI GGNNCC 7 cut(s) 256, 360, 692, 956, 980, 993, 1239
BspMAI CTGCAG 1 cut(s) 1369
BspMI ACCTGC 1 cut(s) 317
BspPI GGATC 2 cut(s) 230, 964
BspT107I GGYRCC 1 cut(s) 1237
BsrDI GCAATG 3 cut(s) 873, 1239, 1353
BsrI ACTGG 5 cut(s) 460, 518, 834, 1285, 1508
BssECI CCNNGG 7 cut(s) 250, 277, 362, 911, 1098, 1422, 1457
BssMI GATC 5 cut(s) 222, 964, 969, 1029, 1330
BssSI CACGAG 1 cut(s) 72
BssT1I CCWWGG 2 cut(s) 911, 1422
Bst2BI CACGAG 1 cut(s) 72
Bst4CI ACNGT 6 cut(s) 706, 782, 1042, 1075, 1111, 1303
Bst6I CTCTTC 1 cut(s) 245
BstC8I GCNNGC 4 cut(s) 440, 631, 1102, 1512
BstDEI CTNAG 4 cut(s) 495, 596, 1403, 1520
BstDSI CCRYGG 2 cut(s) 1098, 1457
BstEII GGTNACC 1 cut(s) 149
BstF5I GGATG 1 cut(s) 1093
BstFNI CGCG 3 cut(s) 744, 1100, 1482
BstH2I RGCGCY 1 cut(s) 1527
BstHHI GCGC 3 cut(s) 744, 1484, 1526
BstKTI GATC 5 cut(s) 225, 967, 972, 1032, 1333
BstMAI GTCTC 1 cut(s) 467
BstMBI GATC 5 cut(s) 222, 964, 969, 1029, 1330
BstMCI CGRYCG 2 cut(s) 283, 1057
BstMWI GCNNNNNNNGC 6 cut(s) 184, 200, 263, 439, 548, 1404
BstPI GGTNACC 1 cut(s) 149
BstSFI CTRYAG 3 cut(s) 528, 601, 1365
BstUI CGCG 3 cut(s) 744, 1100, 1482
BstV1I GCAGC 7 cut(s) 181, 535, 638, 740, 1178, 1351, 1379
BstV2I GAAGAC 1 cut(s) 489
BstX2I RGATCY 1 cut(s) 969
BstXI CCANNNNNNTGG 1 cut(s) 1124
BstYI RGATCY 1 cut(s) 969
BstZI CGGCCG 1 cut(s) 280
BsuRI GGCC 7 cut(s) 141, 228, 257, 282, 426, 633, 1134
BtgI CCRYGG 2 cut(s) 1098, 1457
BtsCI GGATG 1 cut(s) 1093
BtsI GCAGTG 2 cut(s) 621, 808
BtsIMutI CAGTG 5 cut(s) 621, 808, 827, 1252, 1278
BveI ACCTGC 1 cut(s) 317
Cac8I GCNNGC 4 cut(s) 440, 631, 1102, 1512
CaiI CAGNNNCTG 1 cut(s) 1251
CfoI GCGC 3 cut(s) 744, 1484, 1526
Cfr13I GGNCC 3 cut(s) 255, 978, 1132
Cfr42I CCGCGG 1 cut(s) 1101
CseI GACGC 1 cut(s) 879
Csp6I GTAC 4 cut(s) 163, 647, 837, 1238
CspCI CAANNNNNGTGG 2 cut(s) 416, 451
CviAII CATG 4 cut(s) 160, 884, 1156, 1216
CviQI GTAC 4 cut(s) 163, 647, 837, 1238
DdeI CTNAG 4 cut(s) 495, 596, 1403, 1520
DpnI GATC 5 cut(s) 224, 966, 971, 1031, 1332
DpnII GATC 5 cut(s) 222, 964, 969, 1029, 1330
DriI GACNNNNNGTC 1 cut(s) 756
EaeI YGGCCR 2 cut(s) 280, 424
EagI CGGCCG 1 cut(s) 280
Eam1104I CTCTTC 1 cut(s) 245
Eam1105I GACNNNNNGTC 1 cut(s) 756
EarI CTCTTC 1 cut(s) 245
Ecl136II GAGCTC 2 cut(s) 178, 501
EclXI CGGCCG 1 cut(s) 280
Eco130I CCWWGG 2 cut(s) 911, 1422
Eco147I AGGCCT 1 cut(s) 633
Eco24I GRGCYC 3 cut(s) 180, 503, 695
Eco47I GGWCC 1 cut(s) 978
Eco47III AGCGCT 1 cut(s) 1525
Eco52I CGGCCG 1 cut(s) 280
Eco53kI GAGCTC 2 cut(s) 178, 501
Eco88I CYCGRG 1 cut(s) 251
Eco91I GGTNACC 1 cut(s) 149
EcoICRI GAGCTC 2 cut(s) 178, 501
EcoO65I GGTNACC 1 cut(s) 149
EcoT14I CCWWGG 2 cut(s) 911, 1422
EcoT22I ATGCAT 1 cut(s) 1297
EcoT38I GRGCYC 3 cut(s) 180, 503, 695
ErhI CCWWGG 2 cut(s) 911, 1422
FaeI CATG 4 cut(s) 163, 887, 1159, 1219
FaqI GGGAC 1 cut(s) 1052
FatI CATG 4 cut(s) 159, 883, 1155, 1215
FauI CCCGC 2 cut(s) 1093, 1105
FbaI TGATCA 2 cut(s) 964, 1330
FblI GTMKAC 1 cut(s) 774
FokI GGATG 1 cut(s) 1080
FriOI GRGCYC 3 cut(s) 180, 503, 695
FspBI CTAG 2 cut(s) 1016, 1172
GlaI GCGC 3 cut(s) 743, 1483, 1525
GsaI CCCAGC 1 cut(s) 592
HaeII RGCGCY 1 cut(s) 1527
HaeIII GGCC 7 cut(s) 141, 228, 257, 282, 426, 633, 1134
HapII CCGG 1 cut(s) 1057
HgaI GACGC 1 cut(s) 879
HhaI GCGC 3 cut(s) 744, 1484, 1526
Hin1II CATG 4 cut(s) 163, 887, 1159, 1219
Hin6I GCGC 3 cut(s) 742, 1482, 1524
HinP1I GCGC 3 cut(s) 742, 1482, 1524
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 2 cut(s) 1354, 1396
HinfI GANTC 5 cut(s) 269, 654, 907, 1060, 1311
HpaII CCGG 1 cut(s) 1057
HphI GGTGA 6 cut(s) 160, 529, 794, 806, 1291, 1463
Hpy166II GTNNAC 5 cut(s) 214, 775, 811, 1086, 1570
Hpy188III TCNNGA 7 cut(s) 37, 289, 374, 575, 700, 857, 1069
Hpy8I GTNNAC 5 cut(s) 214, 775, 811, 1086, 1570
Hpy99I CGWCG 5 cut(s) 113, 266, 677, 706, 762
HpyAV CCTTC 5 cut(s) 371, 413, 457, 654, 706
HpyCH4III ACNGT 6 cut(s) 706, 782, 1042, 1075, 1111, 1303
HpyCH4IV ACGT 1 cut(s) 210
HpyF10VI GCNNNNNNNGC 6 cut(s) 184, 200, 263, 439, 548, 1404
HpyF3I CTNAG 4 cut(s) 495, 596, 1403, 1520
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 4 cut(s) 163, 887, 1159, 1219
HspAI GCGC 3 cut(s) 742, 1482, 1524
KpnI GGTACC 1 cut(s) 1241
Ksp22I TGATCA 2 cut(s) 964, 1330
KspI CCGCGG 1 cut(s) 1101
Kzo9I GATC 5 cut(s) 222, 964, 969, 1029, 1330
LmnI GCTCC 3 cut(s) 183, 690, 1361
Lsp1109I GCAGC 7 cut(s) 181, 535, 638, 740, 1178, 1351, 1379
LweI GCATC 6 cut(s) 30, 877, 1010, 1282, 1333, 1601
MaeI CTAG 2 cut(s) 1016, 1172
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 6 cut(s) 27, 149, 535, 1033, 1257, 1466
MalI GATC 5 cut(s) 224, 966, 971, 1031, 1332
MboI GATC 5 cut(s) 222, 964, 969, 1029, 1330
MboII GAAGA 4 cut(s) 97, 232, 494, 499
MflI RGATCY 1 cut(s) 969
MhlI GDGCHC 3 cut(s) 180, 503, 695
MluCI AATT 2 cut(s) 845, 1122
MlyI GAGTC 1 cut(s) 1305
MmeI TCCRAC 4 cut(s) 75, 740, 1141, 1372
Mph1103I ATGCAT 1 cut(s) 1297
MslI CAYNNNNRTG 5 cut(s) 38, 372, 704, 1160, 1491
MspA1I CMGCKG 2 cut(s) 194, 1100
MspI CCGG 1 cut(s) 1057
Mva1269I GAATGC 1 cut(s) 1295
MvnI CGCG 3 cut(s) 744, 1100, 1482
MwoI GCNNNNNNNGC 6 cut(s) 184, 200, 263, 439, 548, 1404
NdeII GATC 5 cut(s) 222, 964, 969, 1029, 1330
NlaIII CATG 4 cut(s) 163, 887, 1159, 1219
NlaIV GGNNCC 7 cut(s) 256, 360, 692, 956, 980, 993, 1239
NmeAIII GCCGAG 2 cut(s) 258, 343
NmuCI GTSAC 1 cut(s) 535
NsiI ATGCAT 1 cut(s) 1297
OliI CACNNNNGTG 1 cut(s) 704
PaqCI CACCTGC 1 cut(s) 317
PceI AGGCCT 1 cut(s) 633
PcsI WCGNNNNNNNCGW 3 cut(s) 258, 370, 1038
PctI GAATGC 1 cut(s) 1295
PfeI GAWTC 4 cut(s) 269, 654, 907, 1060
PleI GAGTC 1 cut(s) 1305
PpsI GAGTC 1 cut(s) 1305
Psp124BI GAGCTC 2 cut(s) 180, 503
PspEI GGTNACC 1 cut(s) 149
PspFI CCCAGC 1 cut(s) 588
PspN4I GGNNCC 7 cut(s) 256, 360, 692, 956, 980, 993, 1239
PspPI GGNCC 3 cut(s) 255, 978, 1132
PsrI GAACNNNNNNTAC 2 cut(s) 1026, 1058
PstI CTGCAG 1 cut(s) 1369
PstNI CAGNNNCTG 1 cut(s) 1251
PsuI RGATCY 1 cut(s) 969
RsaI GTAC 4 cut(s) 164, 648, 838, 1239
RsaNI GTAC 4 cut(s) 163, 647, 837, 1238
RseI CAYNNNNRTG 5 cut(s) 38, 372, 704, 1160, 1491
SacI GAGCTC 2 cut(s) 180, 503
SacII CCGCGG 1 cut(s) 1101
Sau3AI GATC 5 cut(s) 222, 964, 969, 1029, 1330
Sau96I GGNCC 3 cut(s) 255, 978, 1132
SchI GAGTC 1 cut(s) 1305
SduI GDGCHC 3 cut(s) 180, 503, 695
SfaNI GCATC 6 cut(s) 30, 877, 1010, 1282, 1333, 1601
SfcI CTRYAG 3 cut(s) 528, 601, 1365
Sfr303I CCGCGG 1 cut(s) 1101
SgrBI CCGCGG 1 cut(s) 1101
SinI GGWCC 1 cut(s) 978
SmiMI CAYNNNNRTG 5 cut(s) 38, 372, 704, 1160, 1491
Sse9I AATT 2 cut(s) 845, 1122
SseBI AGGCCT 1 cut(s) 633
SspMI CTAG 2 cut(s) 1016, 1172
SstI GAGCTC 2 cut(s) 180, 503
StuI AGGCCT 1 cut(s) 633
StyI CCWWGG 2 cut(s) 911, 1422
TaaI ACNGT 6 cut(s) 706, 782, 1042, 1075, 1111, 1303
TaiI ACGT 1 cut(s) 213
TaqI TCGA 1 cut(s) 111
TasI AATT 2 cut(s) 845, 1122
TatI WGTACW 1 cut(s) 646
TauI GCSGC 7 cut(s) 92, 200, 203, 285, 426, 455, 728
TfiI GAWTC 4 cut(s) 269, 654, 907, 1060
TscAI CASTG 5 cut(s) 621, 808, 834, 1252, 1285
TseFI GTSAC 1 cut(s) 535
TseI GCWGC 7 cut(s) 194, 548, 626, 728, 1166, 1364, 1367
Tsp45I GTSAC 1 cut(s) 535
TspDTI ATGAA 4 cut(s) 88, 495, 932, 1402
TspRI CASTG 5 cut(s) 621, 808, 834, 1252, 1285
VpaK11BI GGWCC 1 cut(s) 978
XcmI CCANNNNNNNNNTGG 2 cut(s) 1153, 1248
XmiI GTMKAC 1 cut(s) 774
XspI CTAG 2 cut(s) 1016, 1172
Zsp2I ATGCAT 1 cut(s) 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.