Rh1BG050600

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000830
Physical Location & Seq
Reverse (-)
6944787 .. 6949162
4376 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG050600.1

Sequence Viewer

Length: 1602 bp
ATGGACAGCTTTCACTCTCCAAAGGGCCATCTTCAAGATGAAGATGTTGAGAAGAACATCAGCAGCCAAAAAAAATGTTCCAGAGGTGGGTGGAGTGCTGCCATCTTCATCATCTTTGTTGAAATGGCAGAGAGATTTTCTTACTTTGGCTTGGCTGGTAACCTCATCACCTACCTCACAAATGAACTCCATGAACCAATCCCAACAGCTGCCAAAAACGTCAACACTTGGATTGGTGTCTCATCTCTCCTCCCTATTCTCGGAGCCTTTATCGCTGATTCCTATCTCGGCAGATTCAACACCATCCTTGTCTCCTCCATCATATACTGCATGGCAATGAGTTTGTTAACCGTAACGGTTTCAGCTATTCCACTGCATCTGCGGAAATTAATGTTCTTTGTGGCGCTTTACCTACTGTCACTAGGGCTATCAGGGCATAAACCATGTGTGCAGACGTTCGCGGCCGACCAGTTTTCTGAGGATTCGGCGGAGGAGAGAAAGGCGAAGAGCTCCTTCTTTAACTGGTGGTACTTGGGAATTGTAGTTGGTGGCTCTTCTGCCGTTTTGGTAGTTATATATATCCAGAAATACCGAAGGCAAGGACCCTTGGGCAGTCCCTTCACCAAGGTGGCCCAGGTATTTGTCGCCACCCTCAGGAAGCAGCACATGGAGGTCACAACAGGTCAAGATTTAGATGAGAATTGTCAGCAGCAGCCACATACTACGTTGGTGCATACCAACCAATTCAGATTTTTGGACAAGGCAATGATGATTGACCACCAGGATGCATCAAGCGAGACCACAAATCCTTGGAGATTATGTTCACTAAATCAAGTGGAACAAGTGAAGCTCCTCCTGCGCCTCATTCCCATATGGATGTGCTGCTTAATGTTTGCAGTAGTCCAATCCTTTGTGCAGACCTTCTTCACCAAGCAAGGAAGCACAATGATCCGATCAATCGGATCAATTCAACTTCCCCAGGCTTCACTCCAAATCTTTGTTGGCCTCACCATTGTTGTTGCCATTCCAATCTATGACCGTGTTTTTGTCCCCATTGCTCGAAAATTCACAGGACATTCATCAGGAATTACCATCCTACAAAGAATCGGCACTGGCCTATTAATCTCCATCATGAGCATGGTTGTTGCAGCTGTGGTAGAGACCAAAAGGGTAAAGATTGTAAGAGATCACAACCTCCTGGACAATCCGAAAGCAATAGTACCAATGAGAGTATGGTGGTTAATTCCTCAGTACATGATATGTGGTTTGTCTGACGCATTTGCATTTGTTGGAATGCAAGAGTTGTTCTATGATCAAATGCCAGAGGCAATGAGAAGCTTGGGAGCAGCATTTTACCTCAGTGTTGTTGGGGTTGGATTCTTCTTAAGCAATGGGATAATAACTTTGGTGCAAGCAATCAGCTCAAAGGGTGGTGAAAAATGGATAACTAACAATATCAATCGATCTCACCTGGATTACTTTTATTCGGTAATCGCAGGACTGAGCGCGTTGAATTTCTGCTTGTACGTTTGGATTGCTAAAGGATTTCTATACAAGAGAATCGAAGCCAAAGAATGCAATAAGGAGATAGAACTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

533

Amino Acids

59.78

Weight (kDa)

8.86

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 99 - 196 2.4e-19 POT family
PTR2 PF00854 197 - 477 2.4e-53 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 461, 1508
AciI CCGC 3 cut(s) 382, 461, 488
AclWI GGATC 2 cut(s) 943, 970
AcoI YGGCCR 1 cut(s) 462
AcsI RAATTY 2 cut(s) 1064, 1513
AfaI GTAC 4 cut(s) 530, 1221, 1253, 1526
AfiI CCNNNNNNNGG 3 cut(s) 87, 260, 654
AflII CTTAAG 1 cut(s) 1384
AgsI TTSAA 5 cut(s) 35, 122, 298, 971, 1513
AjnI CCWGG 5 cut(s) 633, 780, 978, 1197, 1470
AleI CACNNNNGTG 1 cut(s) 626
AloI GAACNNNNNNTCC 2 cut(s) 805, 837
AluBI AGCT 8 cut(s) 9, 209, 365, 510, 850, 1151, 1338, 1422
AluI AGCT 8 cut(s) 9, 209, 365, 510, 850, 1151, 1338, 1422
Alw21I GWGCWC 1 cut(s) 512
Alw26I GTCTC 4 cut(s) 244, 316, 791, 1154
AlwI GGATC 2 cut(s) 943, 970
AoxI GGCC 5 cut(s) 25, 462, 630, 1003, 1114
ApeKI GCWGC 9 cut(s) 63, 98, 209, 661, 709, 712, 882, 1148, 1346
ApoI RAATTY 2 cut(s) 1064, 1513
ArsI GACNNNNNNTTYG 2 cut(s) 1028, 1060
AseI ATTAAT 2 cut(s) 389, 1121
AspLEI GCGC 3 cut(s) 406, 861, 1508
AspS9I GGNCC 3 cut(s) 25, 602, 631
AsuHPI GGTGA 6 cut(s) 160, 613, 919, 1000, 1445, 1460
AvaII GGWCC 1 cut(s) 602
AxyI CCTNAGG 1 cut(s) 653
BanII GRGCYC 1 cut(s) 512
Bbv12I GWGCWC 1 cut(s) 512
BbvI GCAGC 9 cut(s) 75, 85, 196, 673, 721, 724, 869, 1160, 1358
BccI CCATC 6 cut(s) 36, 110, 311, 326, 1100, 1136
BceAI ACGGC 1 cut(s) 545
BciT130I CCWGG 5 cut(s) 635, 782, 980, 1199, 1472
BclI TGATCA 1 cut(s) 1312
BcoDI GTCTC 4 cut(s) 244, 316, 791, 1154
BfaI CTAG 1 cut(s) 422
BfoI RGCGCY 1 cut(s) 407
BfrI CTTAAG 1 cut(s) 1384
Bme1390I CCNGG 5 cut(s) 635, 782, 980, 1199, 1472
Bme18I GGWCC 1 cut(s) 602
BmgT120I GGNCC 3 cut(s) 25, 602, 631
BmiI GGNNCC 2 cut(s) 265, 604
BmrFI CCNGG 5 cut(s) 635, 782, 980, 1199, 1472
BmsI GCATC 3 cut(s) 385, 775, 797
Bsa29I ATCGAT 1 cut(s) 1462
BsaBI GATNNNNATC 1 cut(s) 282
BsaI GGTCTC 2 cut(s) 791, 1154
BsaJI CCNNGG 5 cut(s) 606, 624, 633, 809, 978
BsaXI ACNNNNNCTCC 2 cut(s) 805, 835
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 260, 654
Bse1I ACTGG 3 cut(s) 469, 527, 1117
Bse21I CCTNAGG 1 cut(s) 653
Bse3DI GCAATG 5 cut(s) 342, 771, 1053, 1335, 1396
Bse8I GATNNNNATC 1 cut(s) 282
BseBI CCWGG 5 cut(s) 635, 782, 980, 1199, 1472
BseCI ATCGAT 1 cut(s) 1462
BseDI CCNNGG 5 cut(s) 606, 624, 633, 809, 978
BseGI GGATG 4 cut(s) 303, 790, 882, 1092
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 3 cut(s) 87, 260, 654
BseMI GCAATG 5 cut(s) 342, 771, 1053, 1335, 1396
BseMII CTCAG 5 cut(s) 468, 667, 1262, 1372, 1493
BseNI ACTGG 3 cut(s) 469, 527, 1117
BseRI GAGGAG 4 cut(s) 239, 304, 506, 842
BseX3I CGGCCG 1 cut(s) 462
BseXI GCAGC 9 cut(s) 75, 85, 196, 673, 721, 724, 869, 1160, 1358
BsgI GTGCAG 2 cut(s) 470, 935
Bsh1236I CGCG 2 cut(s) 461, 1508
Bsh1285I CGRYCG 1 cut(s) 465
BshFI GGCC 5 cut(s) 27, 464, 632, 1005, 1116
BshVI ATCGAT 1 cut(s) 1462
BsiEI CGRYCG 1 cut(s) 465
BsiHKAI GWGCWC 1 cut(s) 512
BslFI GGGAC 2 cut(s) 600, 1034
BslI CCNNNNNNNGG 3 cut(s) 87, 260, 654
BsmAI GTCTC 4 cut(s) 244, 316, 791, 1154
BsmFI GGGAC 2 cut(s) 600, 1034
BsmI GAATGC 2 cut(s) 1299, 1580
BsnI GGCC 5 cut(s) 27, 464, 632, 1005, 1116
Bso31I GGTCTC 2 cut(s) 791, 1154
Bsp1286I GDGCHC 1 cut(s) 512
Bsp143I GATC 6 cut(s) 948, 953, 962, 1186, 1312, 1463
BspACI CCGC 3 cut(s) 382, 461, 488
BspANI GGCC 5 cut(s) 27, 464, 632, 1005, 1116
BspCNI CTCAG 5 cut(s) 469, 666, 1261, 1371, 1494
BspDI ATCGAT 1 cut(s) 1462
BspFNI CGCG 2 cut(s) 461, 1508
BspHI TCATGA 1 cut(s) 1131
BspLI GGNNCC 2 cut(s) 265, 604
BspPI GGATC 2 cut(s) 943, 970
BspQI GCTCTTC 2 cut(s) 500, 559
BspTI CTTAAG 1 cut(s) 1384
BspTNI GGTCTC 2 cut(s) 791, 1154
BsrDI GCAATG 5 cut(s) 342, 771, 1053, 1335, 1396
BsrI ACTGG 3 cut(s) 469, 527, 1117
BssECI CCNNGG 5 cut(s) 606, 624, 633, 809, 978
BssMI GATC 6 cut(s) 948, 953, 962, 1186, 1312, 1463
BssT1I CCWWGG 3 cut(s) 606, 624, 809
Bst2UI CCWGG 5 cut(s) 635, 782, 980, 1199, 1472
Bst4CI ACNGT 4 cut(s) 352, 358, 417, 1040
Bst6I CTCTTC 2 cut(s) 500, 559
BstAFI CTTAAG 1 cut(s) 1384
BstC8I GCNNGC 1 cut(s) 1413
BstDEI CTNAG 5 cut(s) 477, 653, 1248, 1358, 1502
BstEII GGTNACC 1 cut(s) 158
BstF5I GGATG 4 cut(s) 303, 790, 882, 1092
BstFNI CGCG 2 cut(s) 461, 1508
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 3 cut(s) 406, 861, 1508
BstKTI GATC 6 cut(s) 951, 956, 965, 1189, 1315, 1466
BstMAI GTCTC 4 cut(s) 244, 316, 791, 1154
BstMBI GATC 6 cut(s) 948, 953, 962, 1186, 1312, 1463
BstMCI CGRYCG 1 cut(s) 465
BstMWI GCNNNNNNNGC 3 cut(s) 272, 433, 856
BstNI CCWGG 5 cut(s) 635, 782, 980, 1199, 1472
BstPI GGTNACC 1 cut(s) 158
BstSCI CCNGG 5 cut(s) 633, 780, 978, 1197, 1470
BstUI CGCG 2 cut(s) 461, 1508
BstV1I GCAGC 9 cut(s) 75, 85, 196, 673, 721, 724, 869, 1160, 1358
BstZI CGGCCG 1 cut(s) 462
Bsu15I ATCGAT 1 cut(s) 1462
Bsu36I CCTNAGG 1 cut(s) 653
BsuRI GGCC 5 cut(s) 27, 464, 632, 1005, 1116
BsuTUI ATCGAT 1 cut(s) 1462
BtsCI GGATG 4 cut(s) 303, 790, 882, 1092
BtsI GCAGTG 1 cut(s) 371
BtsIMutI CAGTG 3 cut(s) 371, 1110, 1366
Cac8I GCNNGC 1 cut(s) 1413
CciI TCATGA 1 cut(s) 1131
CfoI GCGC 3 cut(s) 406, 861, 1508
Cfr13I GGNCC 3 cut(s) 25, 602, 631
ClaI ATCGAT 1 cut(s) 1462
CseI GACGC 1 cut(s) 1283
Csp6I GTAC 4 cut(s) 529, 1220, 1252, 1525
CviAII CATG 7 cut(s) 191, 331, 444, 667, 1132, 1138, 1255
CviQI GTAC 4 cut(s) 529, 1220, 1252, 1525
DdeI CTNAG 5 cut(s) 477, 653, 1248, 1358, 1502
DpnI GATC 6 cut(s) 950, 955, 964, 1188, 1314, 1465
DpnII GATC 6 cut(s) 948, 953, 962, 1186, 1312, 1463
EaeI YGGCCR 1 cut(s) 462
EagI CGGCCG 1 cut(s) 462
Eam1104I CTCTTC 2 cut(s) 500, 559
EarI CTCTTC 2 cut(s) 500, 559
EciI GGCGGA 1 cut(s) 503
Ecl136II GAGCTC 1 cut(s) 510
EclXI CGGCCG 1 cut(s) 462
Eco130I CCWWGG 3 cut(s) 606, 624, 809
Eco24I GRGCYC 1 cut(s) 512
Eco31I GGTCTC 2 cut(s) 791, 1154
Eco47I GGWCC 1 cut(s) 602
Eco52I CGGCCG 1 cut(s) 462
Eco53kI GAGCTC 1 cut(s) 510
Eco81I CCTNAGG 1 cut(s) 653
Eco91I GGTNACC 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 510
EcoO109I RGGNCCY 1 cut(s) 602
EcoO65I GGTNACC 1 cut(s) 158
EcoRII CCWGG 5 cut(s) 633, 780, 978, 1197, 1470
EcoT14I CCWWGG 3 cut(s) 606, 624, 809
EcoT22I ATGCAT 1 cut(s) 790
EcoT38I GRGCYC 1 cut(s) 512
ErhI CCWWGG 3 cut(s) 606, 624, 809
FaeI CATG 7 cut(s) 194, 334, 447, 670, 1135, 1141, 1258
FalI AAGNNNNNCTT 2 cut(s) 497, 529
FaqI GGGAC 2 cut(s) 600, 1034
FatI CATG 7 cut(s) 190, 330, 443, 666, 1131, 1137, 1254
FauNDI CATATG 1 cut(s) 872
FbaI TGATCA 1 cut(s) 1312
FokI GGATG 4 cut(s) 290, 797, 889, 1079
FriOI GRGCYC 1 cut(s) 512
FspBI CTAG 1 cut(s) 422
GlaI GCGC 3 cut(s) 405, 860, 1507
HaeII RGCGCY 1 cut(s) 407
HaeIII GGCC 5 cut(s) 27, 464, 632, 1005, 1116
HgaI GACGC 1 cut(s) 1283
HhaI GCGC 3 cut(s) 406, 861, 1508
Hin1II CATG 7 cut(s) 194, 334, 447, 670, 1135, 1141, 1258
Hin6I GCGC 3 cut(s) 404, 859, 1506
HinP1I GCGC 3 cut(s) 404, 859, 1506
HincII GTYRAC 2 cut(s) 223, 348
HindII GTYRAC 2 cut(s) 223, 348
HindIII AAGCTT 1 cut(s) 1336
HinfI GANTC 6 cut(s) 278, 294, 482, 1104, 1377, 1560
HpaI GTTAAC 1 cut(s) 348
HphI GGTGA 6 cut(s) 160, 613, 919, 1000, 1445, 1460
Hpy166II GTNNAC 3 cut(s) 223, 348, 824
Hpy188I TCNGA 7 cut(s) 263, 478, 749, 953, 962, 1209, 1273
Hpy188III TCNNGA 7 cut(s) 35, 81, 583, 655, 686, 1083, 1132
Hpy8I GTNNAC 3 cut(s) 223, 348, 824
HpyAV CCTTC 4 cut(s) 523, 588, 628, 931
HpyCH4III ACNGT 4 cut(s) 352, 358, 417, 1040
HpyCH4IV ACGT 4 cut(s) 219, 455, 725, 1527
HpyF10VI GCNNNNNNNGC 3 cut(s) 272, 433, 856
HpyF3I CTNAG 5 cut(s) 477, 653, 1248, 1358, 1502
HpySE526I ACGT 4 cut(s) 219, 455, 725, 1527
Hsp92II CATG 7 cut(s) 194, 334, 447, 670, 1135, 1141, 1258
HspAI GCGC 3 cut(s) 404, 859, 1506
Ksp22I TGATCA 1 cut(s) 1312
KspAI GTTAAC 1 cut(s) 348
Kzo9I GATC 6 cut(s) 948, 953, 962, 1186, 1312, 1463
LguI GCTCTTC 2 cut(s) 500, 559
LmnI GCTCC 4 cut(s) 263, 515, 855, 1343
Lsp1109I GCAGC 9 cut(s) 75, 85, 196, 673, 721, 724, 869, 1160, 1358
LweI GCATC 3 cut(s) 385, 775, 797
MaeI CTAG 1 cut(s) 422
MaeII ACGT 4 cut(s) 219, 455, 725, 1527
MaeIII GTNAC 4 cut(s) 158, 352, 417, 673
MalI GATC 6 cut(s) 950, 955, 964, 1188, 1314, 1465
MboI GATC 6 cut(s) 948, 953, 962, 1186, 1312, 1463
MboII GAAGA 8 cut(s) 23, 53, 64, 97, 517, 546, 916, 1372
MhlI GDGCHC 1 cut(s) 512
MluCI AATT 9 cut(s) 386, 537, 700, 743, 966, 1064, 1086, 1242, 1513
MmeI TCCRAC 2 cut(s) 1270, 1354
Mph1103I ATGCAT 1 cut(s) 790
MseI TTAA 7 cut(s) 347, 389, 519, 887, 1121, 1241, 1385
MslI CAYNNNNRTG 5 cut(s) 335, 626, 783, 875, 1136
MspA1I CMGCKG 2 cut(s) 209, 1151
MspCI CTTAAG 1 cut(s) 1384
MspR9I CCNGG 5 cut(s) 635, 782, 980, 1199, 1472
Mva1269I GAATGC 2 cut(s) 1299, 1580
MvaI CCWGG 5 cut(s) 635, 782, 980, 1199, 1472
MvnI CGCG 2 cut(s) 461, 1508
MwoI GCNNNNNNNGC 3 cut(s) 272, 433, 856
NdeI CATATG 1 cut(s) 872
NdeII GATC 6 cut(s) 948, 953, 962, 1186, 1312, 1463
NlaIII CATG 7 cut(s) 194, 334, 447, 670, 1135, 1141, 1258
NlaIV GGNNCC 2 cut(s) 265, 604
NmeAIII GCCGAG 1 cut(s) 267
NmuCI GTSAC 2 cut(s) 417, 673
NsiI ATGCAT 1 cut(s) 790
OliI CACNNNNGTG 1 cut(s) 626
PagI TCATGA 1 cut(s) 1131
PciSI GCTCTTC 2 cut(s) 500, 559
PctI GAATGC 2 cut(s) 1299, 1580
PfeI GAWTC 6 cut(s) 278, 294, 482, 1104, 1377, 1560
PfoI TCCNGGA 1 cut(s) 1197
PpuMI RGGWCCY 1 cut(s) 602
PshBI ATTAAT 2 cut(s) 389, 1121
Psp124BI GAGCTC 1 cut(s) 512
Psp5II RGGWCCY 1 cut(s) 602
Psp6I CCWGG 5 cut(s) 633, 780, 978, 1197, 1470
PspEI GGTNACC 1 cut(s) 158
PspGI CCWGG 5 cut(s) 633, 780, 978, 1197, 1470
PspN4I GGNNCC 2 cut(s) 265, 604
PspPI GGNCC 3 cut(s) 25, 602, 631
PspPPI RGGWCCY 1 cut(s) 602
PvuII CAGCTG 2 cut(s) 209, 1151
RsaI GTAC 4 cut(s) 530, 1221, 1253, 1526
RsaNI GTAC 4 cut(s) 529, 1220, 1252, 1525
RseI CAYNNNNRTG 5 cut(s) 335, 626, 783, 875, 1136
SacI GAGCTC 1 cut(s) 512
SapI GCTCTTC 2 cut(s) 500, 559
SaqAI TTAA 7 cut(s) 347, 389, 519, 887, 1121, 1241, 1385
Sau3AI GATC 6 cut(s) 948, 953, 962, 1186, 1312, 1463
Sau96I GGNCC 3 cut(s) 25, 602, 631
ScrFI CCNGG 5 cut(s) 635, 782, 980, 1199, 1472
SduI GDGCHC 1 cut(s) 512
SfaNI GCATC 3 cut(s) 385, 775, 797
SinI GGWCC 1 cut(s) 602
SmiMI CAYNNNNRTG 5 cut(s) 335, 626, 783, 875, 1136
SmlI CTYRAG 1 cut(s) 1384
SmoI CTYRAG 1 cut(s) 1384
Sse9I AATT 9 cut(s) 386, 537, 700, 743, 966, 1064, 1086, 1242, 1513
SsiI CCGC 3 cut(s) 382, 461, 488
SspMI CTAG 1 cut(s) 422
SstI GAGCTC 1 cut(s) 512
StyD4I CCNGG 5 cut(s) 633, 780, 978, 1197, 1470
StyI CCWWGG 3 cut(s) 606, 624, 809
TaaI ACNGT 4 cut(s) 352, 358, 417, 1040
TaiI ACGT 4 cut(s) 222, 458, 728, 1530
TaqI TCGA 3 cut(s) 1060, 1462, 1563
TasI AATT 9 cut(s) 386, 537, 700, 743, 966, 1064, 1086, 1242, 1513
TatI WGTACW 1 cut(s) 1251
TauI GCSGC 1 cut(s) 464
TfiI GAWTC 6 cut(s) 278, 294, 482, 1104, 1377, 1560
Tru1I TTAA 7 cut(s) 347, 389, 519, 887, 1121, 1241, 1385
Tru9I TTAA 7 cut(s) 347, 389, 519, 887, 1121, 1241, 1385
TscAI CASTG 3 cut(s) 378, 1117, 1366
TseFI GTSAC 2 cut(s) 417, 673
TseI GCWGC 9 cut(s) 63, 98, 209, 661, 709, 712, 882, 1148, 1346
Tsp45I GTSAC 2 cut(s) 417, 673
TspDTI ATGAA 5 cut(s) 54, 97, 198, 207, 1068
TspRI CASTG 3 cut(s) 378, 1117, 1366
Vha464I CTTAAG 1 cut(s) 1384
VpaK11BI GGWCC 1 cut(s) 602
VspI ATTAAT 2 cut(s) 389, 1121
XapI RAATTY 2 cut(s) 1064, 1513
XcmI CCANNNNNNNNNTGG 3 cut(s) 998, 1135, 1230
XspI CTAG 1 cut(s) 422
Zsp2I ATGCAT 1 cut(s) 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.