pycom10g26840

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000830
Physical Location & Seq
Forward (+)
28027756 .. 28030550
2795 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g26840.3

Sequence Viewer

Length: 1716 bp
ATGGCGAACTCTGAATCTCCATCTCACCTAAGTATTCACTGTGGCCGTGAAGACACAGAGAAGAATCTCATCAGACAAGAACAACCTTCCAGTTCAAGAGGTGGCTGGAGTGCTGCAATTTTCATCATCTTTGTTGAGATGGCAGAGAGATTTGCTTACTATGGCACGGCTGGAAACCTCATCACATACCTCACAAATGAACTCCATGAGCCCATCCCCATGGCTGCAAAGAATGTCAACACTTGGGTTGGAGTTTCATCTCTCCTCCCAATAATCGGGGCCTTCGTCGCCGATGCCTTTCTCGGTCGATTCAACACCATTCTCGGCTCTTCGATCATTTATTGCTTTGCACTGATAATGTTAACCTTAACGGTTTCAATAATGCCTCGGCATTATCGGAAAGCAATGTTCTTTGTATCACTTTACCTATTGGCAATTGGGCAAGGTGGCCACAAACCATGTGTACAAACCTTTGCGGCCGACCAGTTTGATGAAGACTCGCCGGAGGAGAAGAAGGCGAAGAGCTCATTCTTCAACTGGTGGTACATGGGGATCGTTGTTGGTGCCTCCACTGCTACTTTGGCGGTCATATATGTGCAGGATAATGTTGGGTGGGCGGCGGGGTTTGGAATATTGACAGGGGCAATGGTGGTGGCATTGGTTCTGTTTCTGTTGGGTTACAAGAGATACAGAAAGCAGGGACCCTTAGGCAGCCCCTTCACCAAGGTGGTTCAGGTGCTAGTGGCAGCAGCGAGGAAGCGGTGCGTGGATGGAACACTCATGACTGGTTTTGGTGTGTACAAAGATGAAGAGAGTCAGCATGATACTAGGAGGACTTTAGCACATACCAGTCAATACAGATGCTTGGACAAGGCAATGATCATTGACCAACTTGATGCTTCAAGCATGACCAGAAATCCTTGGAGATTGTGCTCACAAAATCAAATCGAACAAGTAAAGCTCGTCCTACGCCTCATTCCCATATGGTTATGTTGCTTAATGTTTGCTGTAGTCCAATCTTTTAGTGTAACCTTCTTCACGAAGCAAGGAAGCACAATGGTCCGCTCAATTGGTTCGAACTTTAAGCTTCCCCAAGCATCACTTCAAGTCTTCATTGGCCTTACGATTATAGTTGTCGTTCCAATCTATGATCGTGTTTTTGTCCCAGCAGCCCGAAAAATCACGGGACACTCCTCTGGCATCACCACCCTACGAAGAATCGGCATTGGCCTATTTGTATCCATATTCACTATGGTTGTGTCAGCTTTGCTAGAAGCCAAAAGGGTTCGCATTGCAAGAGACCAAAACCTCTTGGACAACCCGAAAACAATAGTGCCAATGAGAGTGTGGTGGTTAATTCCGCAGTACATGATATGTGGTCTGTCTGATGTATTTACATTCGTTGGACTCCAAGAATTCTTCTACGATCAAATGCCGGAGGAAATGAGAAGCATGGGAGCAGCAGCATACCTCAGTGCAATAGGTGTTGGAAACTTCTTGAGCAATGCTATAATATCTGTGGTGCAAGAAATTAGCTCAATGCATGGTGAAAAATGGCTTGGTAACAATATCAATCGTGCCCACCTCGATTCCTTTTATTGGGTAATTGCAGCTTTGGGCACTTTGAATTTGTGCGTTTACGTATTTGTTGCTAAACTTTTCGTATACAAAAAGTTTGAAGCACAAGAAGCCAACAAGGAGAAAGAGCTGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

572

Amino Acids

63.77

Weight (kDa)

9.07

Isoelectric Point (pI)

38.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 563
AccBSI CCGCTC 1 cut(s) 1065
AccI GTMKAC 1 cut(s) 1665
AciI CCGC 7 cut(s) 476, 584, 617, 620, 760, 1063, 1361
AclWI GGATC 1 cut(s) 560
AcoI YGGCCR 3 cut(s) 43, 448, 477
AcsI RAATTY 2 cut(s) 1415, 1627
AfaI GTAC 4 cut(s) 465, 545, 800, 1367
AfiI CCNNNNNNNGG 2 cut(s) 275, 1599
AgsI TTSAA 8 cut(s) 96, 313, 378, 535, 903, 1106, 1627, 1679
AjuI GAANNNNNNNTTGG 4 cut(s) 1086, 1118, 1542, 1574
AleI CACNNNNGTG 1 cut(s) 725
AluBI AGCT 7 cut(s) 525, 961, 1087, 1265, 1536, 1613, 1708
AluI AGCT 7 cut(s) 525, 961, 1087, 1265, 1536, 1613, 1708
Alw21I GWGCWC 2 cut(s) 527, 935
Alw26I GTCTC 1 cut(s) 1293
AlwI GGATC 1 cut(s) 560
AoxI GGCC 6 cut(s) 43, 279, 448, 477, 1117, 1228
ApeKI GCWGC 9 cut(s) 113, 224, 711, 746, 749, 1169, 1460, 1463, 1610
ApoI RAATTY 2 cut(s) 1415, 1627
AspS9I GGNCC 3 cut(s) 279, 701, 1060
AsuHPI GGTGA 4 cut(s) 17, 712, 1195, 1559
AsuII TTCGAA 1 cut(s) 1076
AvaII GGWCC 2 cut(s) 701, 1060
AxyI CCTNAGG 1 cut(s) 706
BaeGI GKGCMC 2 cut(s) 1582, 1622
BalI TGGCCA 1 cut(s) 450
BanI GGYRCC 1 cut(s) 563
BanII GRGCYC 2 cut(s) 213, 527
BbsI GAAGAC 3 cut(s) 57, 501, 1102
Bbv12I GWGCWC 2 cut(s) 527, 935
BbvI GCAGC 9 cut(s) 100, 211, 723, 758, 761, 1181, 1472, 1475, 1622
BccI CCATC 4 cut(s) 28, 133, 221, 764
BceAI ACGGC 2 cut(s) 30, 183
BciVI GTATCC 1 cut(s) 1249
BclI TGATCA 1 cut(s) 879
BcoDI GTCTC 1 cut(s) 1293
BfaI CTAG 3 cut(s) 740, 828, 1271
BfmI CTRYAG 1 cut(s) 1008
BfuI GTATCC 1 cut(s) 1249
Bme18I GGWCC 2 cut(s) 701, 1060
BmgT120I GGNCC 3 cut(s) 279, 701, 1060
BmiI GGNNCC 4 cut(s) 280, 565, 702, 703
BmsI GCATC 5 cut(s) 283, 851, 886, 1106, 1209
BpiI GAAGAC 3 cut(s) 57, 501, 1102
BpmI CTGGAG 1 cut(s) 127
Bpu14I TTCGAA 1 cut(s) 1076
BpuEI CTTGAG 1 cut(s) 1519
BsaAI YACGTR 1 cut(s) 1642
BsaI GGTCTC 1 cut(s) 1293
BsaJI CCNNGG 4 cut(s) 219, 386, 723, 920
Bsc4I CCNNNNNNNGG 2 cut(s) 275, 1599
Bse1I ACTGG 5 cut(s) 90, 484, 542, 790, 849
Bse21I CCTNAGG 1 cut(s) 706
Bse3DI GCAATG 5 cut(s) 411, 651, 882, 1290, 1510
BseDI CCNNGG 4 cut(s) 219, 386, 723, 920
BseGI GGATG 2 cut(s) 213, 775
BseLI CCNNNNNNNGG 2 cut(s) 275, 1599
BseMI GCAATG 5 cut(s) 411, 651, 882, 1290, 1510
BseMII CTCAG 1 cut(s) 1486
BseNI ACTGG 5 cut(s) 90, 484, 542, 790, 849
BseRI GAGGAG 3 cut(s) 254, 521, 1183
BseSI GKGCMC 2 cut(s) 1582, 1622
BseX3I CGGCCG 1 cut(s) 477
BseXI GCAGC 9 cut(s) 100, 211, 723, 758, 761, 1181, 1472, 1475, 1622
BseYI CCCAGC 1 cut(s) 1165
BsgI GTGCAG 1 cut(s) 617
Bsh1285I CGRYCG 2 cut(s) 307, 480
BshFI GGCC 6 cut(s) 45, 281, 450, 479, 1119, 1230
BshNI GGYRCC 1 cut(s) 563
BsiEI CGRYCG 2 cut(s) 307, 480
BsiHKAI GWGCWC 2 cut(s) 527, 935
BsiSI CCGG 2 cut(s) 503, 1436
BslFI GGGAC 3 cut(s) 714, 1148, 1200
BslI CCNNNNNNNGG 2 cut(s) 275, 1599
BsmAI GTCTC 1 cut(s) 1293
BsmFI GGGAC 3 cut(s) 714, 1148, 1200
BsnI GGCC 6 cut(s) 45, 281, 450, 479, 1119, 1230
Bso31I GGTCTC 1 cut(s) 1293
Bsp119I TTCGAA 1 cut(s) 1076
Bsp1286I GDGCHC 5 cut(s) 213, 527, 935, 1582, 1622
Bsp1407I TGTACA 2 cut(s) 463, 798
Bsp143I GATC 5 cut(s) 333, 552, 879, 1150, 1426
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 7 cut(s) 476, 584, 617, 620, 760, 1063, 1361
BspANI GGCC 6 cut(s) 45, 281, 450, 479, 1119, 1230
BspCNI CTCAG 1 cut(s) 1485
BspHI TCATGA 1 cut(s) 780
BspLI GGNNCC 4 cut(s) 280, 565, 702, 703
BspPI GGATC 1 cut(s) 560
BspQI GCTCTTC 2 cut(s) 334, 515
BspT104I TTCGAA 1 cut(s) 1076
BspT107I GGYRCC 1 cut(s) 563
BspTNI GGTCTC 1 cut(s) 1293
BsrBI CCGCTC 1 cut(s) 1065
BsrDI GCAATG 5 cut(s) 411, 651, 882, 1290, 1510
BsrGI TGTACA 2 cut(s) 463, 798
BsrI ACTGG 5 cut(s) 90, 484, 542, 790, 849
BssECI CCNNGG 4 cut(s) 219, 386, 723, 920
BssMI GATC 5 cut(s) 333, 552, 879, 1150, 1426
BssNAI GTATAC 1 cut(s) 1666
BssT1I CCWWGG 3 cut(s) 219, 723, 920
Bst1107I GTATAC 1 cut(s) 1666
Bst4CI ACNGT 2 cut(s) 41, 373
Bst6I CTCTTC 3 cut(s) 334, 515, 804
BstAUI TGTACA 2 cut(s) 463, 798
BstBAI YACGTR 1 cut(s) 1642
BstBI TTCGAA 1 cut(s) 1076
BstDEI CTNAG 3 cut(s) 29, 706, 1472
BstDSI CCRYGG 1 cut(s) 219
BstF5I GGATG 2 cut(s) 213, 775
BstKTI GATC 5 cut(s) 336, 555, 882, 1153, 1429
BstMAI GTCTC 1 cut(s) 1293
BstMBI GATC 5 cut(s) 333, 552, 879, 1150, 1426
BstMCI CGRYCG 2 cut(s) 307, 480
BstMWI GCNNNNNNNGC 4 cut(s) 287, 572, 581, 1688
BstSFI CTRYAG 1 cut(s) 1008
BstSLI GKGCMC 2 cut(s) 1582, 1622
BstSNI TACGTA 1 cut(s) 1642
BstV1I GCAGC 9 cut(s) 100, 211, 723, 758, 761, 1181, 1472, 1475, 1622
BstV2I GAAGAC 3 cut(s) 57, 501, 1102
BstXI CCANNNNNNTGG 1 cut(s) 220
BstZ17I GTATAC 1 cut(s) 1666
BstZI CGGCCG 1 cut(s) 477
Bsu36I CCTNAGG 1 cut(s) 706
BsuI GTATCC 1 cut(s) 1249
BsuRI GGCC 6 cut(s) 45, 281, 450, 479, 1119, 1230
BtgI CCRYGG 1 cut(s) 219
BtsCI GGATG 2 cut(s) 213, 775
BtsI GCAGTG 1 cut(s) 570
BtsIMutI CAGTG 4 cut(s) 37, 350, 570, 1480
CciI TCATGA 1 cut(s) 780
Cfr13I GGNCC 3 cut(s) 279, 701, 1060
Csp6I GTAC 4 cut(s) 464, 544, 799, 1366
CspCI CAANNNNNGTGG 2 cut(s) 633, 668
CviQI GTAC 4 cut(s) 464, 544, 799, 1366
DdeI CTNAG 3 cut(s) 29, 706, 1472
DpnI GATC 5 cut(s) 335, 554, 881, 1152, 1428
DpnII GATC 5 cut(s) 333, 552, 879, 1150, 1426
EaeI YGGCCR 3 cut(s) 43, 448, 477
EagI CGGCCG 1 cut(s) 477
Eam1104I CTCTTC 3 cut(s) 334, 515, 804
EarI CTCTTC 3 cut(s) 334, 515, 804
Ecl136II GAGCTC 1 cut(s) 525
EclXI CGGCCG 1 cut(s) 477
Eco105I TACGTA 1 cut(s) 1642
Eco130I CCWWGG 3 cut(s) 219, 723, 920
Eco24I GRGCYC 2 cut(s) 213, 527
Eco31I GGTCTC 1 cut(s) 1293
Eco47I GGWCC 2 cut(s) 701, 1060
Eco52I CGGCCG 1 cut(s) 477
Eco53kI GAGCTC 1 cut(s) 525
Eco81I CCTNAGG 1 cut(s) 706
EcoICRI GAGCTC 1 cut(s) 525
EcoO109I RGGNCCY 2 cut(s) 279, 701
EcoRI GAATTC 1 cut(s) 1415
EcoT14I CCWWGG 3 cut(s) 219, 723, 920
EcoT22I ATGCAT 1 cut(s) 1545
EcoT38I GRGCYC 2 cut(s) 213, 527
ErhI CCWWGG 3 cut(s) 219, 723, 920
FalI AAGNNNNNCTT 2 cut(s) 1086, 1118
FaqI GGGAC 3 cut(s) 714, 1148, 1200
FauI CCCGC 1 cut(s) 613
FauNDI CATATG 1 cut(s) 983
FbaI TGATCA 1 cut(s) 879
FblI GTMKAC 1 cut(s) 1665
FokI GGATG 2 cut(s) 200, 782
FriOI GRGCYC 2 cut(s) 213, 527
FspBI CTAG 3 cut(s) 740, 828, 1271
GsaI CCCAGC 1 cut(s) 1169
GsuI CTGGAG 1 cut(s) 127
HaeIII GGCC 6 cut(s) 45, 281, 450, 479, 1119, 1230
HapII CCGG 2 cut(s) 503, 1436
HincII GTYRAC 2 cut(s) 238, 363
HindII GTYRAC 2 cut(s) 238, 363
HindIII AAGCTT 1 cut(s) 1085
HinfI GANTC 8 cut(s) 14, 64, 309, 497, 814, 1218, 1407, 1589
HpaI GTTAAC 1 cut(s) 363
HpaII CCGG 2 cut(s) 503, 1436
HphI GGTGA 4 cut(s) 17, 712, 1195, 1559
Hpy166II GTNNAC 6 cut(s) 238, 363, 464, 799, 1639, 1666
Hpy188I TCNGA 4 cut(s) 13, 74, 399, 1387
Hpy188III TCNNGA 4 cut(s) 96, 781, 1039, 1498
Hpy8I GTNNAC 6 cut(s) 238, 363, 464, 799, 1639, 1666
Hpy99I CGWCG 1 cut(s) 290
HpyAV CCTTC 5 cut(s) 96, 292, 508, 727, 1042
HpyCH4III ACNGT 2 cut(s) 41, 373
HpyCH4IV ACGT 1 cut(s) 1641
HpyCH4V TGCA 9 cut(s) 116, 227, 350, 598, 1295, 1478, 1525, 1543, 1610
HpyF10VI GCNNNNNNNGC 4 cut(s) 287, 572, 581, 1688
HpyF3I CTNAG 3 cut(s) 29, 706, 1472
HpySE526I ACGT 1 cut(s) 1641
KflI GGGWCCC 1 cut(s) 701
Ksp22I TGATCA 1 cut(s) 879
KspAI GTTAAC 1 cut(s) 363
Kzo9I GATC 5 cut(s) 333, 552, 879, 1150, 1426
LguI GCTCTTC 2 cut(s) 334, 515
LmnI GCTCC 1 cut(s) 1457
Lsp1109I GCAGC 9 cut(s) 100, 211, 723, 758, 761, 1181, 1472, 1475, 1622
LweI GCATC 5 cut(s) 283, 851, 886, 1106, 1209
MaeI CTAG 3 cut(s) 740, 828, 1271
MaeII ACGT 1 cut(s) 1641
MaeIII GTNAC 3 cut(s) 677, 1027, 1562
MalI GATC 5 cut(s) 335, 554, 881, 1152, 1428
MbiI CCGCTC 1 cut(s) 1065
MboI GATC 5 cut(s) 333, 552, 879, 1150, 1426
MfeI CAATTG 2 cut(s) 435, 1068
MhlI GDGCHC 5 cut(s) 213, 527, 935, 1582, 1622
MlsI TGGCCA 1 cut(s) 450
MluCI AATT 8 cut(s) 117, 435, 1068, 1356, 1415, 1530, 1605, 1627
MluNI TGGCCA 1 cut(s) 450
MlyI GAGTC 3 cut(s) 491, 823, 1401
MmeI TCCRAC 3 cut(s) 229, 1384, 1468
Mox20I TGGCCA 1 cut(s) 450
Mph1103I ATGCAT 1 cut(s) 1545
MscI TGGCCA 1 cut(s) 450
MseI TTAA 5 cut(s) 362, 368, 998, 1083, 1355
MslI CAYNNNNRTG 3 cut(s) 218, 593, 725
Msp20I TGGCCA 1 cut(s) 450
MspI CCGG 2 cut(s) 503, 1436
MunI CAATTG 2 cut(s) 435, 1068
MwoI GCNNNNNNNGC 4 cut(s) 287, 572, 581, 1688
NcoI CCATGG 1 cut(s) 219
NdeI CATATG 1 cut(s) 983
NdeII GATC 5 cut(s) 333, 552, 879, 1150, 1426
NlaIV GGNNCC 4 cut(s) 280, 565, 702, 703
NmeAIII GCCGAG 2 cut(s) 303, 367
NsiI ATGCAT 1 cut(s) 1545
NspV TTCGAA 1 cut(s) 1076
OliI CACNNNNGTG 1 cut(s) 725
PagI TCATGA 1 cut(s) 780
PciSI GCTCTTC 2 cut(s) 334, 515
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PfeI GAWTC 5 cut(s) 14, 64, 309, 1218, 1589
PleI GAGTC 3 cut(s) 491, 822, 1401
PpsI GAGTC 3 cut(s) 491, 822, 1401
Ppu21I YACGTR 1 cut(s) 1642
PpuMI RGGWCCY 1 cut(s) 701
Psp124BI GAGCTC 1 cut(s) 527
Psp5II RGGWCCY 1 cut(s) 701
PspFI CCCAGC 1 cut(s) 1165
PspN4I GGNNCC 4 cut(s) 280, 565, 702, 703
PspPI GGNCC 3 cut(s) 279, 701, 1060
PspPPI RGGWCCY 1 cut(s) 701
RsaI GTAC 4 cut(s) 465, 545, 800, 1367
RsaNI GTAC 4 cut(s) 464, 544, 799, 1366
RseI CAYNNNNRTG 3 cut(s) 218, 593, 725
SacI GAGCTC 1 cut(s) 527
SapI GCTCTTC 2 cut(s) 334, 515
SaqAI TTAA 5 cut(s) 362, 368, 998, 1083, 1355
Sau3AI GATC 5 cut(s) 333, 552, 879, 1150, 1426
Sau96I GGNCC 3 cut(s) 279, 701, 1060
SchI GAGTC 3 cut(s) 491, 823, 1401
SduI GDGCHC 5 cut(s) 213, 527, 935, 1582, 1622
SfaNI GCATC 5 cut(s) 283, 851, 886, 1106, 1209
SfcI CTRYAG 1 cut(s) 1008
SfuI TTCGAA 1 cut(s) 1076
SinI GGWCC 2 cut(s) 701, 1060
SmiMI CAYNNNNRTG 3 cut(s) 218, 593, 725
SmlI CTYRAG 1 cut(s) 1498
SmoI CTYRAG 1 cut(s) 1498
SnaBI TACGTA 1 cut(s) 1642
Sse9I AATT 8 cut(s) 117, 435, 1068, 1356, 1415, 1530, 1605, 1627
SsiI CCGC 7 cut(s) 476, 584, 617, 620, 760, 1063, 1361
SspI AATATT 1 cut(s) 633
SspMI CTAG 3 cut(s) 740, 828, 1271
SstI GAGCTC 1 cut(s) 527
StyI CCWWGG 3 cut(s) 219, 723, 920
TaaI ACNGT 2 cut(s) 41, 373
TaiI ACGT 1 cut(s) 1644
TaqI TCGA 5 cut(s) 307, 332, 948, 1076, 1587
TaqII GACCGA 1 cut(s) 293
TasI AATT 8 cut(s) 117, 435, 1068, 1356, 1415, 1530, 1605, 1627
TatI WGTACW 3 cut(s) 463, 798, 1365
TauI GCSGC 2 cut(s) 479, 620
TfiI GAWTC 5 cut(s) 14, 64, 309, 1218, 1589
Tru1I TTAA 5 cut(s) 362, 368, 998, 1083, 1355
Tru9I TTAA 5 cut(s) 362, 368, 998, 1083, 1355
TscAI CASTG 4 cut(s) 44, 357, 577, 1480
TseI GCWGC 9 cut(s) 113, 224, 711, 746, 749, 1169, 1460, 1463, 1610
TspDTI ATGAA 6 cut(s) 112, 213, 246, 507, 822, 1102
TspRI CASTG 4 cut(s) 44, 357, 577, 1480
VpaK11BI GGWCC 2 cut(s) 701, 1060
XapI RAATTY 2 cut(s) 1415, 1627
XcmI CCANNNNNNNNNTGG 3 cut(s) 577, 1249, 1344
XmiI GTMKAC 1 cut(s) 1665
XspI CTAG 3 cut(s) 740, 828, 1271
Zsp2I ATGCAT 1 cut(s) 1545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.