MD07G1038900.v1.1

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000830
Physical Location & Seq
Reverse (-)
3272734 .. 3276872
4139 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1038900.v1.1.491

Sequence Viewer

Length: 1695 bp
ATGAATATTGCTTCTACTGCACATGGAAAACCCAGTACGGGTGGCTGGAATGCTGCTATTTTCATCATCTTGGTTGAGGTGGCCCAGCAGTTCGCATTCTATGGGTTGGCAAGTAACCTCATCATGTACCTCACAGATGTTTTGGACCAGCCCTTAGCCACGGCGGCTAAGAATGTTAACACCTGGCTTGGTGTTTCATCAGTCTTCCCTGTCGTCGGAGCCTTCCTTGCAGATTCATATTTTGGTCGGTTCAAAACCATCGTCTTCTCTGTCACCATCTATTTCCTGGGCATGGTTCTTTTGACTCTCTCCGTCTCAGTAATTCCTTCACAAAGTCGCCGAGCAATGTTCTTTGTGGCGCTATACATTTTATCGGTGGCTCAAGGTGGGCAGAAGCCATGCGTGCAAACGTTTGCAGCAGACCAGTTCGATGAGAACACGCCGGAAGAGATAAAGGTCAAGAGCTCCTTCTTCAACTGGTGGTACTTGGGGTTGGTGTTCGGTGCCACTTCTGCCACACTAGGAGTTGTATATCTCCAGGATAATGTTGGGTGGGGATTAGGGTTTGGAATATTGGCAGGGGTTTTGGTTTTGTCACTAGTATCGTTCTTGGTGGGAACTAAGAGGTACAGGAAGCAAAGGCCTCCAGGGAGCCCTTTTACAACGGTGGCACAAGTACTCGTTGCAGCAGCTCGGAAATGGCATGTCATTGAGACACTCAATTGCGAGGGAGTTTACTATGGAGATGAGAAGGGTGAAGCCATGCTACAACGTCAGTCGCAGCCTCTAGTTTTGGCTCGTACTAATCAACTCAGATGCTTGGACAAGGCAATGATCATAGACGAACAAGATGCATCAACGAAAACTAGAAATCCATGGAGGCTATGCTCTCTTAATCAAGTGGAAGAAGTGAAGCTTGTCCTTCGACTCATCCCCATATGGTTGAGTTGCCTAATGTTTGGTGTAATCCAAGCCCAACTTCACACCTTTTTCACCAAACAAAGTAGCACAACCATCCGATCGATCGGTCCCCATTTCCAGGTTCCTGCGGCATCACTTCAAGGCCTCGTTGGCATTGCAATCCTAATCGCCGTTCCAATCTACGACCGGGTTTTTGTCCCAGTAGCCAGAAAACATACGGGACACCCTTCGGGTATTACTGTGCTACAAAGAATCGGCATTGGGCTCGTTCTATCCATACTCCTTATGGTTGTGTCGGCTCTAGTAGAGGCCAAAAGGCTCAACATTGCAAAAGACTACAATCTCATTGATAAACCTAAAGCAATAATACCCATGAGGGTATGGTGGTTACTCCCACAATACTTGATTCTTGGCCTCGCAGATGCATTCACCACGGTTGGACTTCAAGAATTGTTCTACGATCAAATGCCGGAGCAAATGAGAAGCATGGGAGCGGCAGCGTCTATGAGTGTTGTAGGAGTTGGTAGCTTCACTAGCAATTGGATTATATCTGCTGCGGAGTTAATTACCTCAAGAAATGGAAACAAATGGCTTGGTGACAATATCAATAGGGCTCACCTCGATTACTTCTATTGGGTGCTGGCAGTTTTGAGCACACTAAATTTTTGTGTTTATGGGGTAATTGCAAGTGCTTTTGTGTACAAAAAGGTCGAAAGAGAAGACAAGGTGAAGACGGAGTTGACCCTTGTAAAGAACCATGATGGAGAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

565

Amino Acids

62.36

Weight (kDa)

9.16

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 29 - 484 4.9e-15 Major Facilitator Superfamily
PTR2 PF00854 84 - 498 7.4e-91 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 503
AccBSI CCGCTC 1 cut(s) 1415
AciI CCGC 4 cut(s) 164, 1049, 1415, 1478
AclI AACGTT 1 cut(s) 410
AcsI RAATTY 1 cut(s) 1582
AfaI GTAC 7 cut(s) 37, 128, 485, 629, 678, 802, 1622
AfiI CCNNNNNNNGG 4 cut(s) 38, 215, 292, 1039
AgsI TTSAA 4 cut(s) 253, 475, 1061, 1367
AhlI ACTAGT 1 cut(s) 598
AjnI CCWGG 5 cut(s) 182, 285, 537, 646, 1038
AjuI GAANNNNNNNTTGG 2 cut(s) 963, 995
AluBI AGCT 4 cut(s) 465, 692, 916, 1449
AluI AGCT 4 cut(s) 465, 692, 916, 1449
Alw21I GWGCWC 2 cut(s) 467, 1577
Alw26I GTCTC 2 cut(s) 319, 707
AoxI GGCC 5 cut(s) 81, 641, 1063, 1230, 1333
ApeKI GCWGC 7 cut(s) 53, 416, 686, 689, 781, 1418, 1475
ApoI RAATTY 1 cut(s) 1582
ArsI GACNNNNNNTTYG 4 cut(s) 246, 278, 1097, 1129
AspLEI GCGC 1 cut(s) 361
AspS9I GGNCC 3 cut(s) 82, 145, 1028
AsuC2I CCSGG 1 cut(s) 1109
AsuHPI GGTGA 7 cut(s) 265, 767, 985, 1342, 1529, 1529, 1660
AvaII GGWCC 2 cut(s) 145, 1028
BanI GGYRCC 1 cut(s) 503
BanII GRGCYC 4 cut(s) 467, 656, 1188, 1537
BarI GAAGNNNNNNTAC 2 cut(s) 750, 782
BbsI GAAGAC 4 cut(s) 196, 256, 1647, 1658
Bbv12I GWGCWC 2 cut(s) 467, 1577
BbvI GCAGC 7 cut(s) 40, 428, 698, 701, 793, 1430, 1462
BccI CCATC 4 cut(s) 266, 284, 1022, 1676
BceAI ACGGC 2 cut(s) 177, 1076
BcgI CGANNNNNNTGC 4 cut(s) 833, 867, 1168, 1202
BciT130I CCWGG 5 cut(s) 184, 287, 539, 648, 1040
BclI TGATCA 1 cut(s) 834
BcnI CCSGG 1 cut(s) 1109
BcoDI GTCTC 2 cut(s) 319, 707
BcuI ACTAGT 1 cut(s) 598
BfaI CTAG 6 cut(s) 521, 599, 788, 867, 1223, 1455
BfoI RGCGCY 1 cut(s) 362
BglI GCCNNNNNGGC 2 cut(s) 164, 1071
BmcAI AGTACT 1 cut(s) 678
Bme1390I CCNGG 6 cut(s) 184, 287, 539, 648, 1040, 1109
Bme18I GGWCC 2 cut(s) 145, 1028
BmgT120I GGNCC 3 cut(s) 82, 145, 1028
BmiI GGNNCC 5 cut(s) 220, 505, 653, 1030, 1044
BmrFI CCNGG 6 cut(s) 184, 287, 539, 648, 1040, 1109
BmrI ACTGGG 2 cut(s) 27, 1115
BmsI GCATC 5 cut(s) 806, 841, 863, 1061, 1333
BmuI ACTGGG 2 cut(s) 27, 1115
BpiI GAAGAC 4 cut(s) 196, 256, 1647, 1658
BpmI CTGGAG 2 cut(s) 521, 630
Bpu10I CCTNAGC 1 cut(s) 154
BpuEI CTTGAG 2 cut(s) 366, 1477
BpuMI CCSGG 1 cut(s) 1109
Bsa29I ATCGAT 1 cut(s) 1022
BsaJI CCNNGG 5 cut(s) 159, 286, 647, 875, 1353
Bsc4I CCNNNNNNNGG 4 cut(s) 38, 215, 292, 1039
Bse1I ACTGG 4 cut(s) 33, 424, 482, 1121
Bse3DI GCAATG 4 cut(s) 351, 837, 1074, 1245
BseBI CCWGG 5 cut(s) 184, 287, 539, 648, 1040
BseCI ATCGAT 1 cut(s) 1022
BseDI CCNNGG 5 cut(s) 159, 286, 647, 875, 1353
BseGI GGATG 2 cut(s) 930, 1014
BseLI CCNNNNNNNGG 4 cut(s) 38, 215, 292, 1039
BseMI GCAATG 4 cut(s) 351, 837, 1074, 1245
BseMII CTCAG 2 cut(s) 330, 826
BseNI ACTGG 4 cut(s) 33, 424, 482, 1121
BseXI GCAGC 7 cut(s) 40, 428, 698, 701, 793, 1430, 1462
BseYI CCCAGC 1 cut(s) 84
Bsh1285I CGRYCG 3 cut(s) 1022, 1026, 1108
BshFI GGCC 5 cut(s) 83, 643, 1065, 1232, 1335
BshNI GGYRCC 1 cut(s) 503
BshVI ATCGAT 1 cut(s) 1022
BsiEI CGRYCG 3 cut(s) 1022, 1026, 1108
BsiHKAI GWGCWC 2 cut(s) 467, 1577
BsiSI CCGG 3 cut(s) 443, 1108, 1391
BslFI GGGAC 3 cut(s) 1014, 1103, 1155
BslI CCNNNNNNNGG 4 cut(s) 38, 215, 292, 1039
BsmAI GTCTC 2 cut(s) 319, 707
BsmBI CGTCTC 1 cut(s) 319
BsmFI GGGAC 3 cut(s) 1014, 1103, 1155
BsmI GAATGC 3 cut(s) 55, 95, 1346
BsnI GGCC 5 cut(s) 83, 643, 1065, 1232, 1335
Bsp1286I GDGCHC 5 cut(s) 467, 656, 1188, 1537, 1577
Bsp1407I TGTACA 1 cut(s) 1620
Bsp143I GATC 4 cut(s) 834, 1019, 1023, 1381
Bsp19I CCATGG 1 cut(s) 875
BspACI CCGC 4 cut(s) 164, 1049, 1415, 1478
BspANI GGCC 5 cut(s) 83, 643, 1065, 1232, 1335
BspCNI CTCAG 2 cut(s) 329, 825
BspDI ATCGAT 1 cut(s) 1022
BspLI GGNNCC 5 cut(s) 220, 505, 653, 1030, 1044
BspT107I GGYRCC 1 cut(s) 503
BsrBI CCGCTC 1 cut(s) 1415
BsrDI GCAATG 4 cut(s) 351, 837, 1074, 1245
BsrGI TGTACA 1 cut(s) 1620
BsrI ACTGG 4 cut(s) 33, 424, 482, 1121
BssECI CCNNGG 5 cut(s) 159, 286, 647, 875, 1353
BssMI GATC 4 cut(s) 834, 1019, 1023, 1381
BssT1I CCWWGG 1 cut(s) 875
Bst2UI CCWGG 5 cut(s) 184, 287, 539, 648, 1040
Bst4CI ACNGT 3 cut(s) 667, 1162, 1357
Bst6I CTCTTC 1 cut(s) 441
BstAUI TGTACA 1 cut(s) 1620
BstC8I GCNNGC 2 cut(s) 404, 1563
BstDEI CTNAG 5 cut(s) 154, 168, 316, 621, 812
BstDSI CCRYGG 3 cut(s) 159, 875, 1353
BstF5I GGATG 2 cut(s) 930, 1014
BstH2I RGCGCY 1 cut(s) 362
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 4 cut(s) 837, 1022, 1026, 1384
BstMAI GTCTC 2 cut(s) 319, 707
BstMBI GATC 4 cut(s) 834, 1019, 1023, 1381
BstMCI CGRYCG 3 cut(s) 1022, 1026, 1108
BstMWI GCNNNNNNNGC 7 cut(s) 17, 164, 227, 403, 512, 1071, 1455
BstNI CCWGG 5 cut(s) 184, 287, 539, 648, 1040
BstNSI RCATGY 1 cut(s) 707
BstSCI CCNGG 6 cut(s) 182, 285, 537, 646, 1038, 1107
BstV1I GCAGC 7 cut(s) 40, 428, 698, 701, 793, 1430, 1462
BstV2I GAAGAC 4 cut(s) 196, 256, 1647, 1658
Bsu15I ATCGAT 1 cut(s) 1022
BsuRI GGCC 5 cut(s) 83, 643, 1065, 1232, 1335
BsuTUI ATCGAT 1 cut(s) 1022
BtgI CCRYGG 3 cut(s) 159, 875, 1353
BtsCI GGATG 2 cut(s) 930, 1014
Cac8I GCNNGC 2 cut(s) 404, 1563
CfoI GCGC 1 cut(s) 361
Cfr13I GGNCC 3 cut(s) 82, 145, 1028
ClaI ATCGAT 1 cut(s) 1022
CseI GACGC 1 cut(s) 1410
Csp6I GTAC 7 cut(s) 36, 127, 484, 628, 677, 801, 1621
CspCI CAANNNNNGTGG 2 cut(s) 1305, 1340
CviQI GTAC 7 cut(s) 36, 127, 484, 628, 677, 801, 1621
DdeI CTNAG 5 cut(s) 154, 168, 316, 621, 812
DpnI GATC 4 cut(s) 836, 1021, 1025, 1383
DpnII GATC 4 cut(s) 834, 1019, 1023, 1381
Eam1104I CTCTTC 1 cut(s) 441
EarI CTCTTC 1 cut(s) 441
Ecl136II GAGCTC 1 cut(s) 465
Eco130I CCWWGG 1 cut(s) 875
Eco147I AGGCCT 2 cut(s) 643, 1065
Eco24I GRGCYC 4 cut(s) 467, 656, 1188, 1537
Eco47I GGWCC 2 cut(s) 145, 1028
Eco53kI GAGCTC 1 cut(s) 465
EcoICRI GAGCTC 1 cut(s) 465
EcoRII CCWGG 5 cut(s) 182, 285, 537, 646, 1038
EcoT14I CCWWGG 1 cut(s) 875
EcoT22I ATGCAT 2 cut(s) 856, 1348
EcoT38I GRGCYC 4 cut(s) 467, 656, 1188, 1537
ErhI CCWWGG 1 cut(s) 875
Esp3I CGTCTC 1 cut(s) 319
FalI AAGNNNNNCTT 6 cut(s) 452, 484, 900, 932, 963, 995
FaqI GGGAC 3 cut(s) 1014, 1103, 1155
FauNDI CATATG 1 cut(s) 938
FbaI TGATCA 1 cut(s) 834
FokI GGATG 2 cut(s) 917, 1001
FriOI GRGCYC 4 cut(s) 467, 656, 1188, 1537
FspBI CTAG 6 cut(s) 521, 599, 788, 867, 1223, 1455
GlaI GCGC 1 cut(s) 360
GsaI CCCAGC 1 cut(s) 88
GsuI CTGGAG 2 cut(s) 521, 630
HaeII RGCGCY 1 cut(s) 362
HaeIII GGCC 5 cut(s) 83, 643, 1065, 1232, 1335
HapII CCGG 3 cut(s) 443, 1108, 1391
HgaI GACGC 1 cut(s) 1410
HhaI GCGC 1 cut(s) 361
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HincII GTYRAC 2 cut(s) 178, 1662
HindII GTYRAC 2 cut(s) 178, 1662
HindIII AAGCTT 1 cut(s) 914
HinfI GANTC 5 cut(s) 233, 304, 927, 1173, 1327
HpaI GTTAAC 1 cut(s) 178
HpaII CCGG 3 cut(s) 443, 1108, 1391
HphI GGTGA 7 cut(s) 265, 767, 985, 1342, 1529, 1529, 1660
Hpy166II GTNNAC 4 cut(s) 178, 736, 1621, 1662
Hpy188I TCNGA 4 cut(s) 218, 696, 815, 1019
Hpy188III TCNNGA 3 cut(s) 460, 1367, 1494
Hpy8I GTNNAC 4 cut(s) 178, 736, 1621, 1662
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 6 cut(s) 232, 336, 478, 745, 932, 1158
HpyCH4III ACNGT 3 cut(s) 667, 1162, 1357
HpyCH4IV ACGT 2 cut(s) 410, 772
HpyF10VI GCNNNNNNNGC 7 cut(s) 17, 164, 227, 403, 512, 1071, 1455
HpyF3I CTNAG 5 cut(s) 154, 168, 316, 621, 812
HpySE526I ACGT 2 cut(s) 410, 772
HspAI GCGC 1 cut(s) 359
Ksp22I TGATCA 1 cut(s) 834
KspAI GTTAAC 1 cut(s) 178
Kzo9I GATC 4 cut(s) 834, 1019, 1023, 1381
LmnI GCTCC 5 cut(s) 218, 470, 651, 1393, 1412
Lsp1109I GCAGC 7 cut(s) 40, 428, 698, 701, 793, 1430, 1462
LweI GCATC 5 cut(s) 806, 841, 863, 1061, 1333
MaeI CTAG 6 cut(s) 521, 599, 788, 867, 1223, 1455
MaeII ACGT 2 cut(s) 410, 772
MaeIII GTNAC 5 cut(s) 113, 271, 594, 1308, 1517
MalI GATC 4 cut(s) 836, 1021, 1025, 1383
MbiI CCGCTC 1 cut(s) 1415
MboI GATC 4 cut(s) 834, 1019, 1023, 1381
MboII GAAGA 7 cut(s) 196, 256, 458, 463, 917, 1652, 1663
MfeI CAATTG 2 cut(s) 721, 1459
MhlI GDGCHC 5 cut(s) 467, 656, 1188, 1537, 1577
MluCI AATT 7 cut(s) 321, 721, 1370, 1459, 1485, 1582, 1602
MlyI GAGTC 2 cut(s) 298, 921
MmeI TCCRAC 2 cut(s) 196, 1339
Mph1103I ATGCAT 2 cut(s) 856, 1348
MseI TTAA 3 cut(s) 177, 894, 1484
MspI CCGG 3 cut(s) 443, 1108, 1391
MspR9I CCNGG 6 cut(s) 184, 287, 539, 648, 1040, 1109
MunI CAATTG 2 cut(s) 721, 1459
Mva1269I GAATGC 3 cut(s) 55, 95, 1346
MvaI CCWGG 5 cut(s) 184, 287, 539, 648, 1040
MwoI GCNNNNNNNGC 7 cut(s) 17, 164, 227, 403, 512, 1071, 1455
NciI CCSGG 1 cut(s) 1109
NcoI CCATGG 1 cut(s) 875
NdeI CATATG 1 cut(s) 938
NdeII GATC 4 cut(s) 834, 1019, 1023, 1381
NlaIV GGNNCC 5 cut(s) 220, 505, 653, 1030, 1044
NmeAIII GCCGAG 1 cut(s) 365
NmuCI GTSAC 3 cut(s) 271, 594, 1517
NsiI ATGCAT 2 cut(s) 856, 1348
NspI RCATGY 1 cut(s) 707
PceI AGGCCT 2 cut(s) 643, 1065
PctI GAATGC 3 cut(s) 55, 95, 1346
PfeI GAWTC 3 cut(s) 233, 1173, 1327
PfoI TCCNGGA 1 cut(s) 537
Ple19I CGATCG 2 cut(s) 1022, 1026
PleI GAGTC 2 cut(s) 298, 921
PpsI GAGTC 2 cut(s) 298, 921
Psp124BI GAGCTC 1 cut(s) 467
Psp1406I AACGTT 1 cut(s) 410
Psp6I CCWGG 5 cut(s) 182, 285, 537, 646, 1038
PspFI CCCAGC 1 cut(s) 84
PspGI CCWGG 5 cut(s) 182, 285, 537, 646, 1038
PspN4I GGNNCC 5 cut(s) 220, 505, 653, 1030, 1044
PspPI GGNCC 3 cut(s) 82, 145, 1028
PvuI CGATCG 2 cut(s) 1022, 1026
RsaI GTAC 7 cut(s) 37, 128, 485, 629, 678, 802, 1622
RsaNI GTAC 7 cut(s) 36, 127, 484, 628, 677, 801, 1621
SacI GAGCTC 1 cut(s) 467
SaqAI TTAA 3 cut(s) 177, 894, 1484
Sau3AI GATC 4 cut(s) 834, 1019, 1023, 1381
Sau96I GGNCC 3 cut(s) 82, 145, 1028
ScaI AGTACT 1 cut(s) 678
SchI GAGTC 2 cut(s) 298, 921
ScrFI CCNGG 6 cut(s) 184, 287, 539, 648, 1040, 1109
SduI GDGCHC 5 cut(s) 467, 656, 1188, 1537, 1577
SfaNI GCATC 5 cut(s) 806, 841, 863, 1061, 1333
SinI GGWCC 2 cut(s) 145, 1028
SmlI CTYRAG 2 cut(s) 381, 1492
SmoI CTYRAG 2 cut(s) 381, 1492
SpeI ACTAGT 1 cut(s) 598
Sse9I AATT 7 cut(s) 321, 721, 1370, 1459, 1485, 1582, 1602
SseBI AGGCCT 2 cut(s) 643, 1065
SsiI CCGC 4 cut(s) 164, 1049, 1415, 1478
SspI AATATT 2 cut(s) 7, 573
SspMI CTAG 6 cut(s) 521, 599, 788, 867, 1223, 1455
SstI GAGCTC 1 cut(s) 467
StuI AGGCCT 2 cut(s) 643, 1065
StyD4I CCNGG 6 cut(s) 182, 285, 537, 646, 1038, 1107
StyI CCWWGG 1 cut(s) 875
TaaI ACNGT 3 cut(s) 667, 1162, 1357
TaiI ACGT 2 cut(s) 413, 775
TaqI TCGA 5 cut(s) 429, 925, 1022, 1542, 1632
TaqII GACCGA 1 cut(s) 1016
TasI AATT 7 cut(s) 321, 721, 1370, 1459, 1485, 1582, 1602
TatI WGTACW 2 cut(s) 676, 1620
TauI GCSGC 3 cut(s) 167, 1052, 1418
TfiI GAWTC 3 cut(s) 233, 1173, 1327
Tru1I TTAA 3 cut(s) 177, 894, 1484
Tru9I TTAA 3 cut(s) 177, 894, 1484
TseFI GTSAC 3 cut(s) 271, 594, 1517
TseI GCWGC 7 cut(s) 53, 416, 686, 689, 781, 1418, 1475
Tsp45I GTSAC 3 cut(s) 271, 594, 1517
TspDTI ATGAA 4 cut(s) 17, 52, 186, 225
TspGWI ACGGA 2 cut(s) 301, 1670
VpaK11BI GGWCC 2 cut(s) 145, 1028
XapI RAATTY 1 cut(s) 1582
XceI RCATGY 1 cut(s) 707
XcmI CCANNNNNNNNNTGG 3 cut(s) 283, 545, 1204
XspI CTAG 6 cut(s) 521, 599, 788, 867, 1223, 1455
ZrmI AGTACT 1 cut(s) 678
Zsp2I ATGCAT 2 cut(s) 856, 1348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.