AT4G23100

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001519
Physical Location & Seq
Reverse (-)
12102135 .. 12107695
5561 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G23100.1

Sequence Viewer

Length: 1569 bp
ATGGCGCTCTTGTCTCAAGCAGGAGGATCATACACTGTTGTTCCTTCTGGAGTTTGTTCAAAGGCTGGAACTAAAGCTGTTGTTTCGGGTGGCGTGAGGAATTTGGATGTTTTGAGGATGAAAGAAGCTTTTGGTAGCTCCTACTCTAGGAGTCTATCTACCAAATCAATGCTTCTCCATTCTGTTAAGAGGAGTAAGAGAGGGCATCAATTGATTGTTGCGGCAAGTCCTCCAACGGAAGAGGCTGTAGTTGCAACTGAGCCGTTGACGAGAGAGGATCTCATTGCCTATCTTGCCTCTGGATGCAAAACAAAGGACAAATATAGAATAGGTACAGAACATGAGAAATTTGGTTTTGAGGTCAATACTTTGCGCCCTATGAAGTATGATCAAATAGCCGAGCTTCTTAATGGTATCGCTGAAAGATTTGAATGGGAAAAAGTAATGGAAGGTGACAAGATCATTGGTCTGAAGCAGGGAAAGCAAAGCATTTCACTTGAACCTGGGGGTCAGTTCGAGCTTAGTGGTGCACCTCTTGAGACTTTGCATCAAACTTGTGCTGAAGTCAATTCACATCTTTATCAGGTAAAAGCAGTTGCTGAGGAAATGGGAATTGGTTTCTTAGGAATTGGCTTCCAGCCCAAATGGCGTCGGGAGGATATACCCATCATGCCAAAGGGGAGATACGACATTATGAGAAACTACATGCCGAAAGTTGGTACCCTTGGTCTTGATATGATGCTCCGAACGTGTACTGTTCAGGTTAATCTGGATTTTAGCTCAGAAGCTGATATGATCAGGAAGTTTCGTGCTGGTCTTGCTTTACAACCTATAGCAACGGCTCTATTTGCGAATTCCCCTTTTACAGAAGGAAAGCCAAACGGATTTCTCAGCATGAGAAGCCACATATGGACAGACACTGACAAGGACCGCACAGGAATGCTACCATTTGTTTTCGATGACTCTTTTGGGTTTGAGCAGTATGTTGACTACGCACTCGATGTCCCTATGTACTTTGCCTACAGAAAGAACAAATACATCGACTGTACTGGAATGACATTTCGGCAATTCTTGGCTGGAAAACTTCCCTGTCTCCCTGGTGAACTGCCTTCATATAATGATTGGGAAAACCATCTGACAACAATATTCCCAGAGGTTCGGTTGAAGAGATACTTGGAGATGAGAGGTGCTGATGGAGGTCCCTGGAGGAGGCTGTGTGCCCTGCCAGCTTTCTGGGTGGGTTTATTATATGATGATGATAGTCTCCAAGCTATCCTGGATCTGACAGCTGACTGGACTCCAGCAGAGAGAGAGATGCTAAGGAACAAAGTCCCAGTTACTGGCTTAAAGACTCCTTTTAGGGATGGTTTGTTAAAGCATGTCGCTGAAGATGTCCTGAAACTCGCAAAGGATGGTTTAGAGCGCAGAGGCTACAAGGAAGCCGGTTTCTTGAACGCAGTCGATGAAGTGGTCAGAACAGGAGTTACGCCTGCGGAGAAGCTCTTGGAGATGTACAATGGAGAATGGGGACAAAGCGTAGATCCCGTGTTCGAAGAGCTGCTGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

522

Amino Acids

58.56

Weight (kDa)

6.16

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 109 - 518 2e-107 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 719
AccB1I GGYRCC 1 cut(s) 719
AccB7I CCANNNNNTGG 1 cut(s) 1340
AciI CCGC 3 cut(s) 221, 931, 1493
AclWI GGATC 4 cut(s) 34, 285, 1287, 1535
AcsI RAATTY 3 cut(s) 100, 347, 853
AcuI CTGAAG 3 cut(s) 491, 582, 1407
AcyI GRCGYC 1 cut(s) 649
AfaI GTAC 7 cut(s) 334, 721, 754, 1013, 1048, 1514, 1565
AfiI CCNNNNNNNGG 4 cut(s) 147, 716, 1209, 1340
AflIII ACRYGT 1 cut(s) 749
AgsI TTSAA 5 cut(s) 60, 431, 500, 1165, 1453
AjnI CCWGG 4 cut(s) 502, 1096, 1202, 1275
AjuI GAANNNNNNNTTGG 4 cut(s) 597, 629, 1157, 1189
Alw21I GWGCWC 1 cut(s) 532
Alw26I GTCTC 4 cut(s) 18, 533, 1097, 1268
Alw44I GTGCAC 1 cut(s) 528
AlwI GGATC 4 cut(s) 34, 285, 1287, 1535
AlwNI CAGNNNCTG 4 cut(s) 599, 788, 920, 1340
ApaLI GTGCAC 1 cut(s) 528
ApeKI GCWGC 1 cut(s) 1558
ApoI RAATTY 3 cut(s) 100, 347, 853
Asp718I GGTACC 1 cut(s) 719
AspLEI GCGC 3 cut(s) 7, 375, 1425
AspS9I GGNCC 2 cut(s) 928, 1199
AsuHPI GGTGA 2 cut(s) 464, 1112
AsuII TTCGAA 1 cut(s) 1551
AvaII GGWCC 2 cut(s) 928, 1199
BaeGI GKGCMC 2 cut(s) 532, 1222
BanI GGYRCC 1 cut(s) 719
Bbv12I GWGCWC 1 cut(s) 532
BbvCI CCTCAGC 1 cut(s) 600
BbvI GCAGC 1 cut(s) 1545
BccI CCATC 5 cut(s) 674, 1140, 1187, 1358, 1406
BceAI ACGGC 2 cut(s) 247, 855
BciT130I CCWGG 4 cut(s) 504, 1098, 1204, 1277
BclI TGATCA 2 cut(s) 388, 795
BcoDI GTCTC 4 cut(s) 18, 533, 1097, 1268
BfaI CTAG 1 cut(s) 147
BfmI CTRYAG 3 cut(s) 246, 831, 1021
BfoI RGCGCY 1 cut(s) 8
BglI GCCNNNNNGGC 1 cut(s) 646
BisI GCNGC 2 cut(s) 222, 1559
BlsI GCNGC 2 cut(s) 223, 1560
Bme1390I CCNGG 4 cut(s) 504, 1098, 1204, 1277
Bme18I GGWCC 2 cut(s) 928, 1199
BmgT120I GGNCC 2 cut(s) 928, 1199
BmiI GGNNCC 2 cut(s) 721, 1201
BmrFI CCNGG 4 cut(s) 504, 1098, 1204, 1277
BmrI ACTGGG 1 cut(s) 1328
BmsI GCATC 5 cut(s) 214, 293, 556, 729, 1305
BmuI ACTGGG 1 cut(s) 1328
BplI GAGNNNNNCTC 2 cut(s) 264, 296
BpmI CTGGAG 3 cut(s) 69, 1225, 1284
Bpu10I CCTNAGC 2 cut(s) 600, 1319
Bpu14I TTCGAA 1 cut(s) 1551
BpuEI CTTGAG 1 cut(s) 557
BsaHI GRCGYC 1 cut(s) 649
BsaJI CCNNGG 4 cut(s) 503, 724, 1096, 1202
BsaXI ACNNNNNCTCC 2 cut(s) 1201, 1231
Bsc4I CCNNNNNNNGG 4 cut(s) 147, 716, 1209, 1340
Bse118I RCCGGY 1 cut(s) 1442
Bse1I ACTGG 4 cut(s) 1054, 1298, 1334, 1345
Bse3DI GCAATG 1 cut(s) 282
BseBI CCWGG 4 cut(s) 504, 1098, 1204, 1277
BseDI CCNNGG 4 cut(s) 503, 724, 1096, 1202
BseGI GGATG 5 cut(s) 112, 123, 308, 1369, 1417
BseLI CCNNNNNNNGG 4 cut(s) 147, 716, 1209, 1340
BseMI GCAATG 1 cut(s) 282
BseMII CTCAG 4 cut(s) 249, 591, 795, 904
BseNI ACTGG 4 cut(s) 1054, 1298, 1334, 1345
BseRI GAGGAG 2 cut(s) 205, 1222
BseSI GKGCMC 2 cut(s) 532, 1222
BseXI GCAGC 1 cut(s) 1545
BshNI GGYRCC 1 cut(s) 719
BsiHKAI GWGCWC 1 cut(s) 532
BsiSI CCGG 1 cut(s) 1443
BslFI GGGAC 4 cut(s) 989, 1185, 1316, 1542
BslI CCNNNNNNNGG 4 cut(s) 147, 716, 1209, 1340
BsmAI GTCTC 4 cut(s) 18, 533, 1097, 1268
BsmFI GGGAC 4 cut(s) 989, 1185, 1316, 1542
BsmI GAATGC 1 cut(s) 945
Bsp119I TTCGAA 1 cut(s) 1551
Bsp1286I GDGCHC 2 cut(s) 532, 1222
Bsp1407I TGTACA 1 cut(s) 1512
Bsp143I GATC 7 cut(s) 26, 277, 388, 459, 795, 1279, 1540
BspACI CCGC 3 cut(s) 221, 931, 1493
BspCNI CTCAG 4 cut(s) 250, 592, 794, 903
BspLI GGNNCC 2 cut(s) 721, 1201
BspPI GGATC 4 cut(s) 34, 285, 1287, 1535
BspQI GCTCTTC 1 cut(s) 1548
BspT104I TTCGAA 1 cut(s) 1551
BspT107I GGYRCC 1 cut(s) 719
BsrDI GCAATG 1 cut(s) 282
BsrFI RCCGGY 1 cut(s) 1442
BsrGI TGTACA 1 cut(s) 1512
BsrI ACTGG 4 cut(s) 1054, 1298, 1334, 1345
BssAI RCCGGY 1 cut(s) 1442
BssECI CCNNGG 4 cut(s) 503, 724, 1096, 1202
BssMI GATC 7 cut(s) 26, 277, 388, 459, 795, 1279, 1540
BssNI GRCGYC 1 cut(s) 649
BssT1I CCWWGG 1 cut(s) 724
Bst2UI CCWGG 4 cut(s) 504, 1098, 1204, 1277
Bst4CI ACNGT 3 cut(s) 37, 757, 1046
Bst6I CTCTTC 3 cut(s) 234, 1160, 1548
BstACI GRCGYC 1 cut(s) 649
BstAUI TGTACA 1 cut(s) 1512
BstBI TTCGAA 1 cut(s) 1551
BstC8I GCNNGC 2 cut(s) 1227, 1491
BstDEI CTNAG 7 cut(s) 258, 521, 600, 622, 781, 890, 1319
BstENI CCTNNNNNAGG 2 cut(s) 145, 1207
BstF5I GGATG 5 cut(s) 112, 123, 308, 1369, 1417
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 375, 1425
BstKTI GATC 7 cut(s) 29, 280, 391, 462, 798, 1282, 1543
BstMAI GTCTC 4 cut(s) 18, 533, 1097, 1268
BstMBI GATC 7 cut(s) 26, 277, 388, 459, 795, 1279, 1540
BstMWI GCNNNNNNNGC 8 cut(s) 251, 293, 481, 646, 818, 848, 900, 1226
BstNI CCWGG 4 cut(s) 504, 1098, 1204, 1277
BstNSI RCATGY 2 cut(s) 709, 1382
BstSCI CCNGG 4 cut(s) 502, 1096, 1202, 1275
BstSFI CTRYAG 3 cut(s) 246, 831, 1021
BstSLI GKGCMC 2 cut(s) 532, 1222
BstV1I GCAGC 1 cut(s) 1545
BstX2I RGATCY 3 cut(s) 277, 1279, 1540
BstXI CCANNNNNNTGG 1 cut(s) 1233
BstYI RGATCY 3 cut(s) 277, 1279, 1540
BtsCI GGATG 5 cut(s) 112, 123, 308, 1369, 1417
BtsIMutI CAGTG 2 cut(s) 33, 918
Cac8I GCNNGC 2 cut(s) 1227, 1491
CaiI CAGNNNCTG 4 cut(s) 599, 788, 920, 1340
CfoI GCGC 3 cut(s) 7, 375, 1425
Cfr10I RCCGGY 1 cut(s) 1442
Cfr13I GGNCC 2 cut(s) 928, 1199
CseI GACGC 1 cut(s) 638
Csp6I GTAC 7 cut(s) 333, 720, 753, 1012, 1047, 1513, 1564
CviAII CATG 5 cut(s) 341, 670, 706, 895, 1379
CviQI GTAC 7 cut(s) 333, 720, 753, 1012, 1047, 1513, 1564
DdeI CTNAG 7 cut(s) 258, 521, 600, 622, 781, 890, 1319
DpnI GATC 7 cut(s) 28, 279, 390, 461, 797, 1281, 1542
DpnII GATC 7 cut(s) 26, 277, 388, 459, 795, 1279, 1540
Eam1104I CTCTTC 3 cut(s) 234, 1160, 1548
EarI CTCTTC 3 cut(s) 234, 1160, 1548
Eco130I CCWWGG 1 cut(s) 724
Eco47I GGWCC 2 cut(s) 928, 1199
Eco57I CTGAAG 3 cut(s) 491, 582, 1407
EcoNI CCTNNNNNAGG 2 cut(s) 145, 1207
EcoO109I RGGNCCY 1 cut(s) 1199
EcoRI GAATTC 1 cut(s) 853
EcoRII CCWGG 4 cut(s) 502, 1096, 1202, 1275
EcoT14I CCWWGG 1 cut(s) 724
ErhI CCWWGG 1 cut(s) 724
FaeI CATG 5 cut(s) 344, 673, 709, 898, 1382
FalI AAGNNNNNCTT 2 cut(s) 1157, 1189
FaqI GGGAC 4 cut(s) 989, 1185, 1316, 1542
FatI CATG 5 cut(s) 340, 669, 705, 894, 1378
FauNDI CATATG 1 cut(s) 908
FbaI TGATCA 2 cut(s) 388, 795
Fnu4HI GCNGC 2 cut(s) 222, 1559
FokI GGATG 5 cut(s) 119, 130, 315, 1376, 1424
Fsp4HI GCNGC 2 cut(s) 222, 1559
FspBI CTAG 1 cut(s) 147
GlaI GCGC 3 cut(s) 6, 374, 1424
GluI GCNGC 2 cut(s) 222, 1559
GsuI CTGGAG 3 cut(s) 69, 1225, 1284
HaeII RGCGCY 1 cut(s) 8
HapII CCGG 1 cut(s) 1443
HgaI GACGC 1 cut(s) 638
HhaI GCGC 3 cut(s) 7, 375, 1425
Hin1I GRCGYC 1 cut(s) 649
Hin1II CATG 5 cut(s) 344, 673, 709, 898, 1382
Hin6I GCGC 3 cut(s) 5, 373, 1423
HinP1I GCGC 3 cut(s) 5, 373, 1423
HincII GTYRAC 2 cut(s) 267, 988
HindII GTYRAC 2 cut(s) 267, 988
HindIII AAGCTT 1 cut(s) 126
HinfI GANTC 4 cut(s) 151, 962, 1297, 1351
HpaII CCGG 1 cut(s) 1443
HphI GGTGA 2 cut(s) 464, 1112
Hpy166II GTNNAC 5 cut(s) 267, 530, 753, 988, 1103
Hpy188I TCNGA 6 cut(s) 471, 746, 784, 1137, 1284, 1475
Hpy188III TCNNGA 9 cut(s) 48, 300, 536, 653, 731, 770, 799, 1396, 1450
Hpy8I GTNNAC 5 cut(s) 267, 530, 753, 988, 1103
Hpy99I CGWCG 1 cut(s) 654
HpyAV CCTTC 4 cut(s) 54, 443, 863, 1119
HpyCH4III ACNGT 3 cut(s) 37, 757, 1046
HpyCH4IV ACGT 1 cut(s) 749
HpyCH4V TGCA 4 cut(s) 254, 306, 530, 547
HpyF10VI GCNNNNNNNGC 8 cut(s) 251, 293, 481, 646, 818, 848, 900, 1226
HpyF3I CTNAG 7 cut(s) 258, 521, 600, 622, 781, 890, 1319
HpySE526I ACGT 1 cut(s) 749
Hsp92I GRCGYC 1 cut(s) 649
Hsp92II CATG 5 cut(s) 344, 673, 709, 898, 1382
HspAI GCGC 3 cut(s) 5, 373, 1423
KpnI GGTACC 1 cut(s) 723
Ksp22I TGATCA 2 cut(s) 388, 795
Kzo9I GATC 7 cut(s) 26, 277, 388, 459, 795, 1279, 1540
LguI GCTCTTC 1 cut(s) 1548
LmnI GCTCC 2 cut(s) 143, 747
Lsp1109I GCAGC 1 cut(s) 1545
LweI GCATC 5 cut(s) 214, 293, 556, 729, 1305
MaeI CTAG 1 cut(s) 147
MaeII ACGT 1 cut(s) 749
MaeIII GTNAC 3 cut(s) 452, 1336, 1483
MalI GATC 7 cut(s) 28, 279, 390, 461, 797, 1281, 1542
MboI GATC 7 cut(s) 26, 277, 388, 459, 795, 1279, 1540
MboII GAAGA 4 cut(s) 251, 1177, 1400, 1565
MfeI CAATTG 1 cut(s) 209
MflI RGATCY 3 cut(s) 277, 1279, 1540
MhlI GDGCHC 2 cut(s) 532, 1222
MluCI AATT 8 cut(s) 100, 209, 347, 568, 612, 627, 853, 1067
MlyI GAGTC 4 cut(s) 160, 956, 1291, 1345
MmeI TCCRAC 1 cut(s) 257
MseI TTAA 5 cut(s) 186, 408, 765, 1346, 1373
MslI CAYNNNNRTG 1 cut(s) 938
MspA1I CMGCKG 1 cut(s) 1289
MspI CCGG 1 cut(s) 1443
MspR9I CCNGG 4 cut(s) 504, 1098, 1204, 1277
MunI CAATTG 1 cut(s) 209
Mva1269I GAATGC 1 cut(s) 945
MvaI CCWGG 4 cut(s) 504, 1098, 1204, 1277
MwoI GCNNNNNNNGC 8 cut(s) 251, 293, 481, 646, 818, 848, 900, 1226
NdeI CATATG 1 cut(s) 908
NdeII GATC 7 cut(s) 26, 277, 388, 459, 795, 1279, 1540
NlaIII CATG 5 cut(s) 344, 673, 709, 898, 1382
NlaIV GGNNCC 2 cut(s) 721, 1201
NmeAIII GCCGAG 1 cut(s) 424
NmuCI GTSAC 1 cut(s) 452
NspI RCATGY 2 cut(s) 709, 1382
NspV TTCGAA 1 cut(s) 1551
PciSI GCTCTTC 1 cut(s) 1548
PctI GAATGC 1 cut(s) 945
PflMI CCANNNNNTGG 1 cut(s) 1340
PfoI TCCNGGA 1 cut(s) 1275
PkrI GCNGC 2 cut(s) 223, 1560
PleI GAGTC 4 cut(s) 159, 956, 1291, 1345
PpsI GAGTC 4 cut(s) 159, 956, 1291, 1345
PpuMI RGGWCCY 1 cut(s) 1199
Psp5II RGGWCCY 1 cut(s) 1199
Psp6I CCWGG 4 cut(s) 502, 1096, 1202, 1275
PspGI CCWGG 4 cut(s) 502, 1096, 1202, 1275
PspN4I GGNNCC 2 cut(s) 721, 1201
PspPI GGNCC 2 cut(s) 928, 1199
PspPPI RGGWCCY 1 cut(s) 1199
PsrI GAACNNNNNNTAC 2 cut(s) 1468, 1500
PstNI CAGNNNCTG 4 cut(s) 599, 788, 920, 1340
PsuI RGATCY 3 cut(s) 277, 1279, 1540
PvuII CAGCTG 1 cut(s) 1289
RsaI GTAC 7 cut(s) 334, 721, 754, 1013, 1048, 1514, 1565
RsaNI GTAC 7 cut(s) 333, 720, 753, 1012, 1047, 1513, 1564
RseI CAYNNNNRTG 1 cut(s) 938
SapI GCTCTTC 1 cut(s) 1548
SaqAI TTAA 5 cut(s) 186, 408, 765, 1346, 1373
SatI GCNGC 2 cut(s) 222, 1559
Sau3AI GATC 7 cut(s) 26, 277, 388, 459, 795, 1279, 1540
Sau96I GGNCC 2 cut(s) 928, 1199
SchI GAGTC 4 cut(s) 160, 956, 1291, 1345
ScrFI CCNGG 4 cut(s) 504, 1098, 1204, 1277
SduI GDGCHC 2 cut(s) 532, 1222
SfaNI GCATC 5 cut(s) 214, 293, 556, 729, 1305
SfcI CTRYAG 3 cut(s) 246, 831, 1021
SfuI TTCGAA 1 cut(s) 1551
SinI GGWCC 2 cut(s) 928, 1199
SmiMI CAYNNNNRTG 1 cut(s) 938
SmlI CTYRAG 2 cut(s) 15, 536
SmoI CTYRAG 2 cut(s) 15, 536
Sse9I AATT 8 cut(s) 100, 209, 347, 568, 612, 627, 853, 1067
SsiI CCGC 3 cut(s) 221, 931, 1493
SspI AATATT 1 cut(s) 1146
SspMI CTAG 1 cut(s) 147
StyD4I CCNGG 4 cut(s) 502, 1096, 1202, 1275
StyI CCWWGG 1 cut(s) 724
TaaI ACNGT 3 cut(s) 37, 757, 1046
TaiI ACGT 1 cut(s) 752
TaqI TCGA 6 cut(s) 516, 957, 999, 1041, 1461, 1551
TasI AATT 8 cut(s) 100, 209, 347, 568, 612, 627, 853, 1067
TatI WGTACW 5 cut(s) 752, 1011, 1046, 1512, 1563
TauI GCSGC 1 cut(s) 224
Tru1I TTAA 5 cut(s) 186, 408, 765, 1346, 1373
Tru9I TTAA 5 cut(s) 186, 408, 765, 1346, 1373
TscAI CASTG 2 cut(s) 40, 925
TseFI GTSAC 1 cut(s) 452
TseI GCWGC 1 cut(s) 1558
Tsp45I GTSAC 1 cut(s) 452
TspDTI ATGAA 4 cut(s) 134, 395, 1101, 1479
TspGWI ACGGA 2 cut(s) 251, 897
TspRI CASTG 2 cut(s) 40, 925
Van91I CCANNNNNTGG 1 cut(s) 1340
VneI GTGCAC 1 cut(s) 528
VpaK11BI GGWCC 2 cut(s) 928, 1199
XagI CCTNNNNNAGG 2 cut(s) 145, 1207
XapI RAATTY 3 cut(s) 100, 347, 853
XceI RCATGY 2 cut(s) 709, 1382
XspI CTAG 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.