Rroxscaffold_3G00258240

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001519
Physical Location & Seq
Reverse (-)
52547625 .. 52553728
6104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00258240.1

Sequence Viewer

Length: 1497 bp
ATGGCACTCATTACACAGACAAGCTCGTCATACTGCATACGTAACGAGATGTTTCAGAATAAAACTGGACAAAATGTGGTGTTTAGTAGGGCAAGCAATATAGAGGCGTCCAAATTGAAGGACAACTACATTGGCTTCTCTTCGTTATTGTGTAATTCTGCTAGGAAGACACAGATTTTTCATACGGACAATGCTGGAGTAGGACAGAAACGACGCCATAGTGTGGTAGTTGCAGCCAGTCCTCCCACAGAAGATGCTGTAATTGCTACTGAACCGCTGACGAAAGAGGATCTTGTAGCGTATCTTGCCTCTGGATGCAAGCCTAAGGAAGCATGGAGAATTGGCACTGAACACGAGAAGTTTGGTTTTGAACTCAAATCTTTACTTCCAGTGAAATATGAACAAATATCTGAGTTGCTTAATGGGATTGCCGAGAGATTTGATTGGGAAAAAGTTATGGAAGGCGACTACATAATTGGACTCAAACAGGGTAAGCAAAGTATATCACTTGAGCCTGGTGGCCAATTTGAGCTTAGTGGCGCACCTCTTGAAACTCTGCATCAAACTTGTGCTGAAGTCAATTCACACCTCTATCAGGTCAAAGCAGTTACAGAGGAAATGGGAATTGGATTTCTTGGCATTGGTTTTCAGCCTAAATGGGGACTGAAGGACATACCTATAATGCCCAAGGTGAATCTGGACTTCAGCTCTGAAGCAGACATGATAAGGAAATTTCGTGCTGGTCTTGCTTTGCAGCCTATTGCAACAGCTCTATTTGCAAATTCACCTTTTACCGAAGGAAAGCCAAATGGTTTTCTGAGCATGAGAAGTCAAATTTGGACCGATACTGACAACAATCGCACTGGCATGCTTCCTTTTGTTTTTGATGACTCCTTTGGATTTGAGCAGTATGTTGATTATGCTCTTGATGTTCCCATGTACTTTGTCTATCGGAATAAAAAGTATATTGACTGTACTGGCATGACCTTCCGGGACTTTTTGGCTGGGAAGCTTCGTTGCATTCCTGGTGAATTGCCAACCCTGAATGATTGGGAGAATCATTTGACAACTATATTTCCTGAGGTCAGGCTGAAGAGATACTTGGAGATGAGGGGAGCTGATGGAGGGCCTTGGAGGAGATTATGTGCTTTGCCAGCATTTTGGGTAGGTATACTGTACGATGAGGTTTCCCTTCAAAATGTGTTGGACATTACTGCTGATTGGACCCCTGAAGAAAGACAGATGCTAAGAAATAAGGTTCCAATAACGGGTCTAAAGACACCATTTAGGGATGGATTGCTCAAGCATGTTGCTCAAGATGTTGTAAAGTTGGCAAAGGATGGACTTGAAAGAAGAGGCTTTAAGGAATCTGGCTTCTTGAATGAGGTGGCAGAGGTGGTTAGAACTGGTGTAACACCAGCTGAGAGATTATTGGAGTTGTACAATGGGAAGTGGGAACGACAGATAGACCCTGTTTTTGAAGAGCTACTATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

498

Amino Acids

56.32

Weight (kDa)

5.72

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 113 - 230 9.7e-18 Glutamate-cysteine ligase family 2(GCS2)
GCS2 PF04107 229 - 494 9e-65 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 25
AccB7I CCANNNNNTGG 1 cut(s) 223
AccI GTMKAC 1 cut(s) 1171
AciI CCGC 1 cut(s) 275
AclWI GGATC 1 cut(s) 297
AcoI YGGCCR 1 cut(s) 520
AcsI RAATTY 3 cut(s) 731, 781, 834
AcuI CTGAAG 6 cut(s) 594, 686, 688, 732, 1112, 1251
AcyI GRCGYC 2 cut(s) 107, 214
AfaI GTAC 4 cut(s) 941, 976, 1178, 1442
AfiI CCNNNNNNNGG 4 cut(s) 223, 595, 659, 1268
AgsI TTSAA 7 cut(s) 118, 371, 551, 1196, 1349, 1381, 1481
AjnI CCWGG 2 cut(s) 514, 1024
AjuI GAANNNNNNNTTGG 6 cut(s) 609, 641, 820, 852, 1085, 1117
AluBI AGCT 8 cut(s) 24, 532, 708, 770, 1012, 1118, 1421, 1486
AluI AGCT 8 cut(s) 24, 532, 708, 770, 1012, 1118, 1421, 1486
AlwI GGATC 1 cut(s) 297
AoxI GGCC 2 cut(s) 520, 1127
ApeKI GCWGC 2 cut(s) 233, 754
ApoI RAATTY 3 cut(s) 731, 781, 834
ArsI GACNNNNNNTTYG 2 cut(s) 1460, 1492
AspLEI GCGC 1 cut(s) 542
AspS9I GGNCC 3 cut(s) 840, 1127, 1224
AsuC2I CCSGG 1 cut(s) 992
AsuHPI GGTGA 3 cut(s) 703, 777, 1040
AvaII GGWCC 2 cut(s) 840, 1224
AxyI CCTNAGG 2 cut(s) 324, 1080
BalI TGGCCA 1 cut(s) 522
BarI GAAGNNNNNNTAC 2 cut(s) 110, 142
BauI CACGAG 1 cut(s) 353
BbsI GAAGAC 1 cut(s) 173
BbvI GCAGC 2 cut(s) 245, 766
BccI CCATC 3 cut(s) 1115, 1286, 1334
BciT130I CCWGG 2 cut(s) 516, 1026
BcnI CCSGG 1 cut(s) 992
BfaI CTAG 1 cut(s) 162
BisI GCNGC 2 cut(s) 234, 755
BlsI GCNGC 2 cut(s) 235, 756
Bme1390I CCNGG 3 cut(s) 516, 992, 1026
Bme18I GGWCC 2 cut(s) 840, 1224
BmgT120I GGNCC 3 cut(s) 840, 1127, 1224
BmiI GGNNCC 2 cut(s) 1226, 1260
BmrFI CCNGG 3 cut(s) 516, 992, 1026
BmsI GCATC 4 cut(s) 244, 305, 568, 1233
BpiI GAAGAC 1 cut(s) 173
BpmI CTGGAG 1 cut(s) 216
BpuEI CTTGAG 3 cut(s) 530, 1286, 1299
BpuMI CCSGG 1 cut(s) 992
BsaAI YACGTR 1 cut(s) 41
BsaHI GRCGYC 2 cut(s) 107, 214
BsaJI CCNNGG 2 cut(s) 687, 1130
BsaXI ACNNNNNCTCC 2 cut(s) 1129, 1159
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 595, 659, 1268
Bse1I ACTGG 6 cut(s) 70, 237, 389, 868, 982, 1411
Bse21I CCTNAGG 2 cut(s) 324, 1080
BseBI CCWGG 2 cut(s) 516, 1026
BseDI CCNNGG 2 cut(s) 687, 1130
BseGI GGATG 3 cut(s) 320, 1297, 1345
BseLI CCNNNNNNNGG 4 cut(s) 223, 595, 659, 1268
BseMII CTCAG 4 cut(s) 402, 809, 1071, 1413
BseNI ACTGG 6 cut(s) 70, 237, 389, 868, 982, 1411
BseRI GAGGAG 1 cut(s) 1150
BseXI GCAGC 2 cut(s) 245, 766
BseYI CCCAGC 1 cut(s) 1004
BshFI GGCC 2 cut(s) 522, 1129
BsiSI CCGG 1 cut(s) 991
BslFI GGGAC 2 cut(s) 675, 1007
BslI CCNNNNNNNGG 4 cut(s) 223, 595, 659, 1268
BsmFI GGGAC 2 cut(s) 675, 1007
BsmI GAATGC 1 cut(s) 1020
BsnI GGCC 2 cut(s) 522, 1129
Bsp1407I TGTACA 1 cut(s) 1440
Bsp143I GATC 1 cut(s) 289
BspACI CCGC 1 cut(s) 275
BspANI GGCC 2 cut(s) 522, 1129
BspCNI CTCAG 4 cut(s) 403, 810, 1072, 1414
BspLI GGNNCC 2 cut(s) 1226, 1260
BspPI GGATC 1 cut(s) 297
BspQI GCTCTTC 1 cut(s) 1476
BsrGI TGTACA 1 cut(s) 1440
BsrI ACTGG 6 cut(s) 70, 237, 389, 868, 982, 1411
BssECI CCNNGG 2 cut(s) 687, 1130
BssMI GATC 1 cut(s) 289
BssNAI GTATAC 1 cut(s) 1172
BssNI GRCGYC 2 cut(s) 107, 214
BssSI CACGAG 1 cut(s) 353
BssT1I CCWWGG 2 cut(s) 687, 1130
Bst1107I GTATAC 1 cut(s) 1172
Bst2BI CACGAG 1 cut(s) 353
Bst2UI CCWGG 2 cut(s) 516, 1026
Bst4CI ACNGT 2 cut(s) 974, 1176
Bst6I CTCTTC 4 cut(s) 145, 1088, 1348, 1476
BstACI GRCGYC 2 cut(s) 107, 214
BstAUI TGTACA 1 cut(s) 1440
BstBAI YACGTR 1 cut(s) 41
BstC8I GCNNGC 4 cut(s) 94, 320, 869, 1155
BstDEI CTNAG 7 cut(s) 324, 411, 533, 818, 1080, 1247, 1422
BstENI CCTNNNNNAGG 1 cut(s) 593
BstF5I GGATG 3 cut(s) 320, 1297, 1345
BstHHI GCGC 1 cut(s) 542
BstKTI GATC 1 cut(s) 292
BstMBI GATC 1 cut(s) 289
BstMWI GCNNNNNNNGC 5 cut(s) 263, 305, 746, 776, 1154
BstNI CCWGG 2 cut(s) 516, 1026
BstNSI RCATGY 2 cut(s) 871, 1310
BstSCI CCNGG 3 cut(s) 514, 990, 1024
BstSNI TACGTA 1 cut(s) 41
BstV1I GCAGC 2 cut(s) 245, 766
BstV2I GAAGAC 1 cut(s) 173
BstX2I RGATCY 1 cut(s) 289
BstXI CCANNNNNNTGG 1 cut(s) 1161
BstYI RGATCY 1 cut(s) 289
BstZ17I GTATAC 1 cut(s) 1172
Bsu36I CCTNAGG 2 cut(s) 324, 1080
BsuRI GGCC 2 cut(s) 522, 1129
BtsCI GGATG 3 cut(s) 320, 1297, 1345
BtsIMutI CAGTG 3 cut(s) 345, 396, 861
Cac8I GCNNGC 4 cut(s) 94, 320, 869, 1155
CfoI GCGC 1 cut(s) 542
Cfr13I GGNCC 3 cut(s) 840, 1127, 1224
CseI GACGC 2 cut(s) 96, 222
Csp6I GTAC 4 cut(s) 940, 975, 1177, 1441
CviAII CATG 7 cut(s) 333, 721, 823, 868, 937, 982, 1307
CviQI GTAC 4 cut(s) 940, 975, 1177, 1441
DdeI CTNAG 7 cut(s) 324, 411, 533, 818, 1080, 1247, 1422
DpnI GATC 1 cut(s) 291
DpnII GATC 1 cut(s) 289
DrdI GACNNNNNNGTC 1 cut(s) 25
DseDI GACNNNNNNGTC 1 cut(s) 25
EaeI YGGCCR 1 cut(s) 520
Eam1104I CTCTTC 4 cut(s) 145, 1088, 1348, 1476
EarI CTCTTC 4 cut(s) 145, 1088, 1348, 1476
Eco105I TACGTA 1 cut(s) 41
Eco130I CCWWGG 2 cut(s) 687, 1130
Eco47I GGWCC 2 cut(s) 840, 1224
Eco57I CTGAAG 6 cut(s) 594, 686, 688, 732, 1112, 1251
Eco81I CCTNAGG 2 cut(s) 324, 1080
EcoNI CCTNNNNNAGG 1 cut(s) 593
EcoO109I RGGNCCY 1 cut(s) 1127
EcoRII CCWGG 2 cut(s) 514, 1024
EcoT14I CCWWGG 2 cut(s) 687, 1130
ErhI CCWWGG 2 cut(s) 687, 1130
FaeI CATG 7 cut(s) 336, 724, 826, 871, 940, 985, 1310
FalI AAGNNNNNCTT 4 cut(s) 276, 308, 1085, 1117
FaqI GGGAC 2 cut(s) 675, 1007
FatI CATG 7 cut(s) 332, 720, 822, 867, 936, 981, 1306
FblI GTMKAC 1 cut(s) 1171
Fnu4HI GCNGC 2 cut(s) 234, 755
FokI GGATG 3 cut(s) 327, 1304, 1352
Fsp4HI GCNGC 2 cut(s) 234, 755
FspBI CTAG 1 cut(s) 162
GlaI GCGC 1 cut(s) 541
GluI GCNGC 2 cut(s) 234, 755
GsaI CCCAGC 1 cut(s) 1008
GsuI CTGGAG 1 cut(s) 216
HaeIII GGCC 2 cut(s) 522, 1129
HapII CCGG 1 cut(s) 991
HgaI GACGC 2 cut(s) 96, 222
HhaI GCGC 1 cut(s) 542
Hin1I GRCGYC 2 cut(s) 107, 214
Hin1II CATG 7 cut(s) 336, 724, 826, 871, 940, 985, 1310
Hin6I GCGC 1 cut(s) 540
HinP1I GCGC 1 cut(s) 540
HindIII AAGCTT 1 cut(s) 1010
HinfI GANTC 5 cut(s) 480, 694, 890, 1057, 1367
HpaII CCGG 1 cut(s) 991
HphI GGTGA 3 cut(s) 703, 777, 1040
Hpy166II GTNNAC 1 cut(s) 1172
Hpy188I TCNGA 5 cut(s) 57, 412, 712, 819, 954
Hpy188III TCNNGA 7 cut(s) 312, 548, 698, 926, 1079, 1316, 1378
Hpy8I GTNNAC 1 cut(s) 1172
Hpy99I CGWCG 1 cut(s) 216
HpyAV CCTTC 6 cut(s) 112, 455, 661, 791, 997, 1202
HpyCH4III ACNGT 2 cut(s) 974, 1176
HpyCH4IV ACGT 1 cut(s) 40
HpyCH4V TGCA 8 cut(s) 36, 233, 318, 559, 754, 764, 779, 1020
HpyF10VI GCNNNNNNNGC 5 cut(s) 263, 305, 746, 776, 1154
HpyF3I CTNAG 7 cut(s) 324, 411, 533, 818, 1080, 1247, 1422
HpySE526I ACGT 1 cut(s) 40
Hsp92I GRCGYC 2 cut(s) 107, 214
Hsp92II CATG 7 cut(s) 336, 724, 826, 871, 940, 985, 1310
HspAI GCGC 1 cut(s) 540
Kzo9I GATC 1 cut(s) 289
LguI GCTCTTC 1 cut(s) 1476
LmnI GCTCC 1 cut(s) 1115
Lsp1109I GCAGC 2 cut(s) 245, 766
LweI GCATC 4 cut(s) 244, 305, 568, 1233
MaeI CTAG 1 cut(s) 162
MaeII ACGT 1 cut(s) 40
MaeIII GTNAC 3 cut(s) 41, 607, 1411
MalI GATC 1 cut(s) 291
MboI GATC 1 cut(s) 289
MboII GAAGA 7 cut(s) 132, 178, 263, 1105, 1244, 1365, 1493
MflI RGATCY 1 cut(s) 289
MlsI TGGCCA 1 cut(s) 522
MluNI TGGCCA 1 cut(s) 522
MlyI GAGTC 2 cut(s) 474, 884
MmeI TCCRAC 1 cut(s) 1185
Mox20I TGGCCA 1 cut(s) 522
MscI TGGCCA 1 cut(s) 522
MseI TTAA 2 cut(s) 420, 1362
MslI CAYNNNNRTG 1 cut(s) 866
Msp20I TGGCCA 1 cut(s) 522
MspA1I CMGCKG 2 cut(s) 277, 1421
MspI CCGG 1 cut(s) 991
MspR9I CCNGG 3 cut(s) 516, 992, 1026
Mva1269I GAATGC 1 cut(s) 1020
MvaI CCWGG 2 cut(s) 516, 1026
MwoI GCNNNNNNNGC 5 cut(s) 263, 305, 746, 776, 1154
NciI CCSGG 1 cut(s) 992
NdeII GATC 1 cut(s) 289
NlaIII CATG 7 cut(s) 336, 724, 826, 871, 940, 985, 1310
NlaIV GGNNCC 2 cut(s) 1226, 1260
NmeAIII GCCGAG 1 cut(s) 457
NspI RCATGY 2 cut(s) 871, 1310
PaeI GCATGC 1 cut(s) 871
PciSI GCTCTTC 1 cut(s) 1476
PctI GAATGC 1 cut(s) 1020
PfeI GAWTC 3 cut(s) 694, 1057, 1367
PflMI CCANNNNNTGG 1 cut(s) 223
PfoI TCCNGGA 1 cut(s) 990
PkrI GCNGC 2 cut(s) 235, 756
PleI GAGTC 2 cut(s) 474, 884
PpsI GAGTC 2 cut(s) 474, 884
Ppu21I YACGTR 1 cut(s) 41
Psp6I CCWGG 2 cut(s) 514, 1024
PspFI CCCAGC 1 cut(s) 1004
PspGI CCWGG 2 cut(s) 514, 1024
PspN4I GGNNCC 2 cut(s) 1226, 1260
PspPI GGNCC 3 cut(s) 840, 1127, 1224
PsuI RGATCY 1 cut(s) 289
PvuII CAGCTG 1 cut(s) 1421
RsaI GTAC 4 cut(s) 941, 976, 1178, 1442
RsaNI GTAC 4 cut(s) 940, 975, 1177, 1441
RseI CAYNNNNRTG 1 cut(s) 866
SapI GCTCTTC 1 cut(s) 1476
SaqAI TTAA 2 cut(s) 420, 1362
SatI GCNGC 2 cut(s) 234, 755
Sau3AI GATC 1 cut(s) 289
Sau96I GGNCC 3 cut(s) 840, 1127, 1224
SchI GAGTC 2 cut(s) 474, 884
ScrFI CCNGG 3 cut(s) 516, 992, 1026
SfaNI GCATC 4 cut(s) 244, 305, 568, 1233
SinI GGWCC 2 cut(s) 840, 1224
SmiMI CAYNNNNRTG 1 cut(s) 866
SmlI CTYRAG 3 cut(s) 509, 1301, 1314
SmoI CTYRAG 3 cut(s) 509, 1301, 1314
SnaBI TACGTA 1 cut(s) 41
SphI GCATGC 1 cut(s) 871
SsiI CCGC 1 cut(s) 275
SspMI CTAG 1 cut(s) 162
StyD4I CCNGG 3 cut(s) 514, 990, 1024
StyI CCWWGG 2 cut(s) 687, 1130
TaaI ACNGT 2 cut(s) 974, 1176
TaiI ACGT 1 cut(s) 43
TaqII GACCGA 1 cut(s) 857
TatI WGTACW 3 cut(s) 939, 974, 1440
TfiI GAWTC 3 cut(s) 694, 1057, 1367
Tru1I TTAA 2 cut(s) 420, 1362
Tru9I TTAA 2 cut(s) 420, 1362
TscAI CASTG 3 cut(s) 352, 396, 868
TseI GCWGC 2 cut(s) 233, 754
TspDTI ATGAA 2 cut(s) 170, 414
TspGWI ACGGA 1 cut(s) 200
TspRI CASTG 3 cut(s) 352, 396, 868
Van91I CCANNNNNTGG 1 cut(s) 223
VpaK11BI GGWCC 2 cut(s) 840, 1224
XagI CCTNNNNNAGG 1 cut(s) 593
XapI RAATTY 3 cut(s) 731, 781, 834
XceI RCATGY 2 cut(s) 871, 1310
XcmI CCANNNNNNNNNTGG 1 cut(s) 694
XmiI GTMKAC 1 cut(s) 1171
XspI CTAG 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.