RLG00000003910

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001519
Physical Location & Seq
Reverse (-)
54215798 .. 54219720
3923 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003910

Sequence Viewer

Length: 1149 bp
ATGGCACTCATTTCCCAGACAATTTCGTCATACTACTGCAGAAGCATTGGCTTCTCTTCCTCTGCATTTTATTCTACTACTACTAGGAAGATACAGGTTTTGCATGTAGAAAATGTTGCAGGACAGAAACGAGGACGTAAAAGTGCGATAGTTGCTGCAAGTCCTATCACAGAGGATGCTGTAGTAGCTACAAAGCCGCTGATGAAAGAAGATCTCGTAGGCTATCTTGCCTCTGGATGCAAGCCCAAAGAAAAATGGAGAATTGGCATGGAACACGAGAGGTTTGGCTTCGAACTAAATACTTTACGTCCGCTGCAATATGAACAAATAGCTGAGCTGCTCTATGGGATTGCCGGGAGATTTGAGTGGGAGAAAATCATGGAAGGTGGTAAGATTATTGGACTCAAACAGGGTAAGCAGAGCATATCACTAGAGCCAGGAGGTCAATTTGAGCTTAGTGGTGCACCTCTTGAAACTCTGCATCAAATTTGTATTGAAGTCAATTCACAACTCTGTCAGGTCAAAGCAGTTGCAGAGAAAATGGGAATCGGATTTCTTGAAATTGGTGGTCAGCCGAAATGGAGAATTCAAGACATACCTATGATGCCCAAGGGAAGATATGAGATTATTAGAAATTACATGCCAAAAGTTGGCTCGCTTGGGCATGATTTGATGTTCAGAACTTGCACTGTACAGGTCAATCTGGACTTCAGTTCTGAAGCAGACATGAAACGGAAATTGCGTGCTAGTCTTGCTTTGCAGCCTATAGCAACAGCCCTATTTGCAAATTCACCTTTCACTGAAGGAAAACCGAATGGTTTTCTAAGCAGGAGAAGTCACATCTACACTGATACTGACAAGGATCGCACTGGCATGCTTCCTTTTGCTTTTGATGACTCCTTTGGGTTTGAGCGCTATGTTGATTATGCTCTTGATGTTCCAATGTACTGTGTTTATCGGAAAAATAAGTGTATTGACTGTACTGGAATGACCTTTCGGGACTTTATGGCAGGAAAGCTTCGTTGCCTTCCAGGTGAATTACCTACACTGAATGATTGGGAGAATCATTTGGGAACTATTTATCCAGAGGTACCAATAATAATCAATCATCAGTCTATAACGTTAGGATTTTCTGATGCTATTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

383

Amino Acids

42.92

Weight (kDa)

7.99

Isoelectric Point (pI)

41.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 87 - 364 1.5e-71 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 25
Acc65I GGTACC 1 cut(s) 1090
AccB1I GGYRCC 1 cut(s) 1090
AccB7I CCANNNNNTGG 1 cut(s) 650
AciI CCGC 2 cut(s) 197, 311
AclI AACGTT 1 cut(s) 1121
AclWI GGATC 1 cut(s) 870
AcsI RAATTY 3 cut(s) 486, 585, 787
AcuI CTGAAG 3 cut(s) 694, 738, 822
AfaI GTAC 4 cut(s) 693, 947, 982, 1092
AfeI AGCGCT 1 cut(s) 914
AfiI CCNNNNNNNGG 1 cut(s) 650
AgsI TTSAA 4 cut(s) 473, 497, 560, 590
AjnI CCWGG 2 cut(s) 436, 1030
AloI GAACNNNNNNTCC 2 cut(s) 1066, 1098
AluBI AGCT 5 cut(s) 188, 332, 337, 454, 1018
AluI AGCT 5 cut(s) 188, 332, 337, 454, 1018
Alw21I GWGCWC 1 cut(s) 466
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 1 cut(s) 870
Aor51HI AGCGCT 1 cut(s) 914
ApaLI GTGCAC 1 cut(s) 462
ApeKI GCWGC 4 cut(s) 155, 313, 337, 760
ApoI RAATTY 3 cut(s) 486, 585, 787
Asp718I GGTACC 1 cut(s) 1090
AspLEI GCGC 1 cut(s) 915
AsuC2I CCSGG 1 cut(s) 355
AsuHPI GGTGA 2 cut(s) 783, 1046
AsuII TTCGAA 1 cut(s) 291
BaeGI GKGCMC 1 cut(s) 466
BanI GGYRCC 1 cut(s) 1090
BauI CACGAG 1 cut(s) 275
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 4 cut(s) 142, 300, 324, 772
BciT130I CCWGG 2 cut(s) 438, 1032
BcnI CCSGG 1 cut(s) 355
BfaI CTAG 3 cut(s) 84, 431, 747
BfmI CTRYAG 3 cut(s) 37, 180, 765
BfoI RGCGCY 1 cut(s) 916
BglII AGATCT 1 cut(s) 211
BisI GCNGC 5 cut(s) 156, 197, 314, 338, 761
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 5 cut(s) 157, 198, 315, 339, 762
Bme1390I CCNGG 3 cut(s) 355, 438, 1032
BmiI GGNNCC 1 cut(s) 1092
BmrFI CCNGG 3 cut(s) 355, 438, 1032
BmsI GCATC 5 cut(s) 166, 227, 490, 594, 1126
Bpu1102I GCTNAGC 1 cut(s) 333
Bpu14I TTCGAA 1 cut(s) 291
BpuMI CCSGG 1 cut(s) 355
BsaJI CCNNGG 1 cut(s) 609
Bsc4I CCNNNNNNNGG 1 cut(s) 650
Bse1I ACTGG 2 cut(s) 874, 988
BseBI CCWGG 2 cut(s) 438, 1032
BseDI CCNNGG 1 cut(s) 609
BseGI GGATG 2 cut(s) 181, 242
BseLI CCNNNNNNNGG 1 cut(s) 650
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 2 cut(s) 874, 988
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 4 cut(s) 142, 300, 324, 772
BshNI GGYRCC 1 cut(s) 1090
BsiHKAI GWGCWC 1 cut(s) 466
BsiSI CCGG 1 cut(s) 354
BslFI GGGAC 1 cut(s) 1013
BslI CCNNNNNNNGG 1 cut(s) 650
BsmFI GGGAC 1 cut(s) 1013
Bsp119I TTCGAA 1 cut(s) 291
Bsp1286I GDGCHC 1 cut(s) 466
Bsp1407I TGTACA 1 cut(s) 691
Bsp143I GATC 2 cut(s) 211, 862
Bsp1720I GCTNAGC 1 cut(s) 333
BspACI CCGC 2 cut(s) 197, 311
BspCNI CTCAG 1 cut(s) 325
BspLI GGNNCC 1 cut(s) 1092
BspMAI CTGCAG 1 cut(s) 41
BspPI GGATC 1 cut(s) 870
BspT104I TTCGAA 1 cut(s) 291
BspT107I GGYRCC 1 cut(s) 1090
BsrGI TGTACA 1 cut(s) 691
BsrI ACTGG 2 cut(s) 874, 988
BssECI CCNNGG 1 cut(s) 609
BssMI GATC 2 cut(s) 211, 862
BssSI CACGAG 1 cut(s) 275
BssT1I CCWWGG 1 cut(s) 609
Bst2BI CACGAG 1 cut(s) 275
Bst2UI CCWGG 2 cut(s) 438, 1032
Bst4CI ACNGT 3 cut(s) 691, 950, 980
Bst6I CTCTTC 1 cut(s) 61
BstAUI TGTACA 1 cut(s) 691
BstBI TTCGAA 1 cut(s) 291
BstC8I GCNNGC 4 cut(s) 242, 656, 744, 875
BstDEI CTNAG 3 cut(s) 333, 455, 824
BstF5I GGATG 2 cut(s) 181, 242
BstH2I RGCGCY 1 cut(s) 916
BstHHI GCGC 1 cut(s) 915
BstKTI GATC 2 cut(s) 214, 865
BstMBI GATC 2 cut(s) 211, 862
BstMWI GCNNNNNNNGC 4 cut(s) 152, 185, 752, 782
BstNI CCWGG 2 cut(s) 438, 1032
BstNSI RCATGY 3 cut(s) 107, 643, 877
BstSCI CCNGG 3 cut(s) 353, 436, 1030
BstSFI CTRYAG 3 cut(s) 37, 180, 765
BstSLI GKGCMC 1 cut(s) 466
BstV1I GCAGC 4 cut(s) 142, 300, 324, 772
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BtsCI GGATG 2 cut(s) 181, 242
BtsIMutI CAGTG 5 cut(s) 687, 798, 846, 867, 1046
Cac8I GCNNGC 4 cut(s) 242, 656, 744, 875
CfoI GCGC 1 cut(s) 915
Csp6I GTAC 4 cut(s) 692, 946, 981, 1091
CviAII CATG 7 cut(s) 104, 268, 379, 640, 665, 727, 874
CviQI GTAC 4 cut(s) 692, 946, 981, 1091
DdeI CTNAG 3 cut(s) 333, 455, 824
DpnI GATC 2 cut(s) 213, 864
DpnII GATC 2 cut(s) 211, 862
DrdI GACNNNNNNGTC 1 cut(s) 25
DseDI GACNNNNNNGTC 1 cut(s) 25
Eam1104I CTCTTC 1 cut(s) 61
EarI CTCTTC 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 609
Eco47III AGCGCT 1 cut(s) 914
Eco57I CTGAAG 3 cut(s) 694, 738, 822
EcoRI GAATTC 1 cut(s) 585
EcoRII CCWGG 2 cut(s) 436, 1030
EcoT14I CCWWGG 1 cut(s) 609
ErhI CCWWGG 1 cut(s) 609
FaeI CATG 7 cut(s) 107, 271, 382, 643, 668, 730, 877
FaqI GGGAC 1 cut(s) 1013
FatI CATG 7 cut(s) 103, 267, 378, 639, 664, 726, 873
Fnu4HI GCNGC 5 cut(s) 156, 197, 314, 338, 761
FokI GGATG 2 cut(s) 188, 249
Fsp4HI GCNGC 5 cut(s) 156, 197, 314, 338, 761
FspBI CTAG 3 cut(s) 84, 431, 747
GlaI GCGC 1 cut(s) 914
GluI GCNGC 5 cut(s) 156, 197, 314, 338, 761
HaeII RGCGCY 1 cut(s) 916
HapII CCGG 1 cut(s) 354
HhaI GCGC 1 cut(s) 915
Hin1II CATG 7 cut(s) 107, 271, 382, 643, 668, 730, 877
Hin6I GCGC 1 cut(s) 913
HinP1I GCGC 1 cut(s) 913
HindIII AAGCTT 1 cut(s) 1016
HinfI GANTC 4 cut(s) 402, 546, 896, 1063
HpaII CCGG 1 cut(s) 354
HphI GGTGA 2 cut(s) 783, 1046
Hpy166II GTNNAC 1 cut(s) 464
Hpy188I TCNGA 5 cut(s) 551, 680, 718, 960, 1135
Hpy188III TCNNGA 8 cut(s) 234, 470, 557, 590, 704, 932, 998, 1085
Hpy8I GTNNAC 1 cut(s) 464
HpyAV CCTTC 3 cut(s) 377, 797, 1037
HpyCH4III ACNGT 3 cut(s) 691, 950, 980
HpyCH4IV ACGT 3 cut(s) 136, 307, 1121
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 185, 752, 782
HpyF3I CTNAG 3 cut(s) 333, 455, 824
HpySE526I ACGT 3 cut(s) 136, 307, 1121
Hsp92II CATG 7 cut(s) 107, 271, 382, 643, 668, 730, 877
HspAI GCGC 1 cut(s) 913
KpnI GGTACC 1 cut(s) 1094
Kzo9I GATC 2 cut(s) 211, 862
Lsp1109I GCAGC 4 cut(s) 142, 300, 324, 772
LweI GCATC 5 cut(s) 166, 227, 490, 594, 1126
MaeI CTAG 3 cut(s) 84, 431, 747
MaeII ACGT 3 cut(s) 136, 307, 1121
MaeIII GTNAC 1 cut(s) 836
MalI GATC 2 cut(s) 213, 864
MboI GATC 2 cut(s) 211, 862
MboII GAAGA 4 cut(s) 48, 100, 221, 627
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 1 cut(s) 466
MlyI GAGTC 2 cut(s) 396, 890
MnlI CCTC 8 cut(s) 70, 125, 166, 241, 273, 434, 477, 1081
MslI CAYNNNNRTG 2 cut(s) 599, 872
MspA1I CMGCKG 2 cut(s) 199, 313
MspI CCGG 1 cut(s) 354
MspR9I CCNGG 3 cut(s) 355, 438, 1032
MvaI CCWGG 2 cut(s) 438, 1032
MwoI GCNNNNNNNGC 4 cut(s) 152, 185, 752, 782
NciI CCSGG 1 cut(s) 355
NdeII GATC 2 cut(s) 211, 862
NlaIII CATG 7 cut(s) 107, 271, 382, 643, 668, 730, 877
NlaIV GGNNCC 1 cut(s) 1092
NmuCI GTSAC 1 cut(s) 836
NspI RCATGY 3 cut(s) 107, 643, 877
NspV TTCGAA 1 cut(s) 291
PaeI GCATGC 1 cut(s) 877
PcsI WCGNNNNNNNCGW 1 cut(s) 739
PfeI GAWTC 2 cut(s) 546, 1063
PflMI CCANNNNNTGG 1 cut(s) 650
PkrI GCNGC 5 cut(s) 157, 198, 315, 339, 762
PleI GAGTC 2 cut(s) 396, 890
PpsI GAGTC 2 cut(s) 396, 890
Psp1406I AACGTT 1 cut(s) 1121
Psp6I CCWGG 2 cut(s) 436, 1030
PspGI CCWGG 2 cut(s) 436, 1030
PspN4I GGNNCC 1 cut(s) 1092
PstI CTGCAG 1 cut(s) 41
PsuI RGATCY 1 cut(s) 211
RsaI GTAC 4 cut(s) 693, 947, 982, 1092
RsaNI GTAC 4 cut(s) 692, 946, 981, 1091
RseI CAYNNNNRTG 2 cut(s) 599, 872
SatI GCNGC 5 cut(s) 156, 197, 314, 338, 761
Sau3AI GATC 2 cut(s) 211, 862
SchI GAGTC 2 cut(s) 396, 890
ScrFI CCNGG 3 cut(s) 355, 438, 1032
SduI GDGCHC 1 cut(s) 466
SfaNI GCATC 5 cut(s) 166, 227, 490, 594, 1126
SfcI CTRYAG 3 cut(s) 37, 180, 765
SfuI TTCGAA 1 cut(s) 291
SmiMI CAYNNNNRTG 2 cut(s) 599, 872
SphI GCATGC 1 cut(s) 877
SsiI CCGC 2 cut(s) 197, 311
SspMI CTAG 3 cut(s) 84, 431, 747
StyD4I CCNGG 3 cut(s) 353, 436, 1030
StyI CCWWGG 1 cut(s) 609
TaaI ACNGT 3 cut(s) 691, 950, 980
TaiI ACGT 3 cut(s) 139, 310, 1124
TaqI TCGA 1 cut(s) 291
TatI WGTACW 3 cut(s) 691, 945, 980
TauI GCSGC 1 cut(s) 199
TfiI GAWTC 2 cut(s) 546, 1063
TscAI CASTG 5 cut(s) 694, 805, 853, 874, 1053
TseFI GTSAC 1 cut(s) 836
TseI GCWGC 4 cut(s) 155, 313, 337, 760
Tsp45I GTSAC 1 cut(s) 836
TspDTI ATGAA 3 cut(s) 218, 336, 743
TspGWI ACGGA 1 cut(s) 748
TspRI CASTG 5 cut(s) 694, 805, 853, 874, 1053
Van91I CCANNNNNTGG 1 cut(s) 650
VneI GTGCAC 1 cut(s) 462
XapI RAATTY 3 cut(s) 486, 585, 787
XceI RCATGY 3 cut(s) 107, 643, 877
XspI CTAG 3 cut(s) 84, 431, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.