Rorug07G0043400

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001519
Physical Location & Seq
Forward (+)
3180259 .. 3180597
339 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0043400.1

Sequence Viewer

Length: 339 bp
ATGCATATAAGCAACAACACCTTCGAGCTTTTTAGATCCCAGGTCTTCCTCGATGATGCCATTTGTTTCGAACTCTTTCCTCCAATCCAAGCACTTGATTATCATGTTGAAAGTGTCGACGACCCAGAAATCCCAAGCTTAGAGAAAATGGAGGAGGATGACGTCAGTCTTGGTATCTCAGGCGAACAATTTGACACCCCACATGGCCCCGTTGCTGGATTCAGAGGCAATAAGCTTCTTGTTGAGCTCCTAGAGCGCCTTCTCCGACGACTTGAGACTCGAGATGAAGTAGGTGACTTCCTTGAAAGACGGTGTTCGGAAGGTGGCGACCTCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

112

Amino Acids

12.8

Weight (kDa)

4.39

Isoelectric Point (pI)

54.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 165
AccI GTMKAC 1 cut(s) 117
AclWI GGATC 1 cut(s) 30
AcyI GRCGYC 1 cut(s) 162
AfiI CCNNNNNNNGG 1 cut(s) 215
AgsI TTSAA 2 cut(s) 110, 305
AjnI CCWGG 1 cut(s) 39
AluBI AGCT 4 cut(s) 28, 138, 235, 247
AluI AGCT 4 cut(s) 28, 138, 235, 247
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 1 cut(s) 30
Ama87I CYCGRG 1 cut(s) 279
AoxI GGCC 1 cut(s) 205
Asp700I GAANNNNTTC 1 cut(s) 75
AspLEI GCGC 1 cut(s) 258
AspS9I GGNCC 1 cut(s) 206
AsuHPI GGTGA 1 cut(s) 305
AsuII TTCGAA 1 cut(s) 69
AvaI CYCGRG 1 cut(s) 279
BanII GRGCYC 1 cut(s) 249
BbsI GAAGAC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 249
BciT130I CCWGG 1 cut(s) 41
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 1 cut(s) 251
BfoI RGCGCY 1 cut(s) 259
Bme1390I CCNGG 1 cut(s) 41
BmeT110I CYCGRG 1 cut(s) 279
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 1 cut(s) 208
BmrFI CCNGG 1 cut(s) 41
BmsI GCATC 1 cut(s) 46
BoxI GACNNNNGTC 1 cut(s) 165
BpiI GAAGAC 1 cut(s) 37
Bpu14I TTCGAA 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 293
BsaHI GRCGYC 1 cut(s) 162
BsaJI CCNNGG 1 cut(s) 39
Bsc4I CCNNNNNNNGG 1 cut(s) 215
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 39
BseGI GGATG 1 cut(s) 163
BseLI CCNNNNNNNGG 1 cut(s) 215
BseMII CTCAG 1 cut(s) 192
BseRI GAGGAG 1 cut(s) 167
BshFI GGCC 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 249
BsiHKCI CYCGRG 1 cut(s) 279
BslI CCNNNNNNNGG 1 cut(s) 215
BsmAI GTCTC 1 cut(s) 269
BsnI GGCC 1 cut(s) 207
BsoBI CYCGRG 1 cut(s) 279
Bsp119I TTCGAA 1 cut(s) 69
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 1 cut(s) 35
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 1 cut(s) 191
BspLI GGNNCC 1 cut(s) 208
BspPI GGATC 1 cut(s) 30
BspT104I TTCGAA 1 cut(s) 69
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 1 cut(s) 35
BssNI GRCGYC 1 cut(s) 162
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 312
BstACI GRCGYC 1 cut(s) 162
BstBI TTCGAA 1 cut(s) 69
BstDEI CTNAG 2 cut(s) 139, 178
BstF5I GGATG 1 cut(s) 163
BstH2I RGCGCY 1 cut(s) 259
BstHHI GCGC 1 cut(s) 258
BstKTI GATC 1 cut(s) 38
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 1 cut(s) 35
BstMWI GCNNNNNNNGC 1 cut(s) 253
BstNI CCWGG 1 cut(s) 41
BstPAI GACNNNNGTC 1 cut(s) 165
BstSCI CCNGG 1 cut(s) 39
BstV2I GAAGAC 1 cut(s) 37
BstX2I RGATCY 1 cut(s) 35
BstYI RGATCY 1 cut(s) 35
BsuRI GGCC 1 cut(s) 207
BtsCI GGATG 1 cut(s) 163
CfoI GCGC 1 cut(s) 258
Cfr13I GGNCC 1 cut(s) 206
CviAII CATG 2 cut(s) 104, 203
CviJI RGCY 5 cut(s) 28, 138, 207, 235, 247
CviKI_1 RGCY 5 cut(s) 28, 138, 207, 235, 247
DdeI CTNAG 2 cut(s) 139, 178
DpnI GATC 1 cut(s) 37
DpnII GATC 1 cut(s) 35
Ecl136II GAGCTC 1 cut(s) 247
Eco24I GRGCYC 1 cut(s) 249
Eco53kI GAGCTC 1 cut(s) 247
Eco88I CYCGRG 1 cut(s) 279
EcoICRI GAGCTC 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 39
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 249
FaeI CATG 2 cut(s) 107, 206
FaiI YATR 4 cut(s) 6, 8, 105, 204
FatI CATG 2 cut(s) 103, 202
FblI GTMKAC 1 cut(s) 117
FokI GGATG 1 cut(s) 170
FriOI GRGCYC 1 cut(s) 249
FspBI CTAG 1 cut(s) 251
GlaI GCGC 1 cut(s) 257
HaeII RGCGCY 1 cut(s) 259
HaeIII GGCC 1 cut(s) 207
HhaI GCGC 1 cut(s) 258
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 2 cut(s) 107, 206
Hin6I GCGC 1 cut(s) 256
HinP1I GCGC 1 cut(s) 256
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HindIII AAGCTT 2 cut(s) 136, 233
HinfI GANTC 2 cut(s) 219, 277
HphI GGTGA 1 cut(s) 305
Hpy166II GTNNAC 1 cut(s) 118
Hpy188I TCNGA 3 cut(s) 224, 266, 319
Hpy188III TCNNGA 1 cut(s) 281
Hpy8I GTNNAC 1 cut(s) 118
Hpy99I CGWCG 2 cut(s) 122, 270
HpyAV CCTTC 3 cut(s) 31, 269, 314
HpyCH4III ACNGT 1 cut(s) 312
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 253
HpyF3I CTNAG 2 cut(s) 139, 178
HpySE526I ACGT 1 cut(s) 162
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 2 cut(s) 107, 206
HspAI GCGC 1 cut(s) 256
Kzo9I GATC 1 cut(s) 35
LmnI GCTCC 1 cut(s) 252
LpnPI CCDG 5 cut(s) 26, 53, 138, 165, 201
LweI GCATC 1 cut(s) 46
MaeI CTAG 1 cut(s) 251
MaeII ACGT 1 cut(s) 162
MaeIII GTNAC 1 cut(s) 293
MalI GATC 1 cut(s) 37
MboI GATC 1 cut(s) 35
MboII GAAGA 1 cut(s) 37
MflI RGATCY 1 cut(s) 35
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 1 cut(s) 188
MlyI GAGTC 1 cut(s) 271
MmeI TCCRAC 1 cut(s) 289
MnlI CCTC 5 cut(s) 59, 90, 145, 148, 218
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 75
MspR9I CCNGG 1 cut(s) 41
MvaI CCWGG 1 cut(s) 41
MwoI GCNNNNNNNGC 1 cut(s) 253
NdeII GATC 1 cut(s) 35
NlaIII CATG 2 cut(s) 107, 206
NlaIV GGNNCC 1 cut(s) 208
NmuCI GTSAC 1 cut(s) 293
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 69
PaeR7I CTCGAG 1 cut(s) 279
PdmI GAANNNNTTC 1 cut(s) 75
PfeI GAWTC 1 cut(s) 219
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
PshAI GACNNNNGTC 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 39
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 1 cut(s) 208
PspPI GGNCC 1 cut(s) 206
PsuI RGATCY 1 cut(s) 35
SacI GAGCTC 1 cut(s) 249
SalI GTCGAC 1 cut(s) 116
Sau3AI GATC 1 cut(s) 35
Sau96I GGNCC 1 cut(s) 206
SchI GAGTC 1 cut(s) 271
ScrFI CCNGG 1 cut(s) 41
SduI GDGCHC 1 cut(s) 249
SfaNI GCATC 1 cut(s) 46
Sfr274I CTCGAG 1 cut(s) 279
SfuI TTCGAA 1 cut(s) 69
SlaI CTCGAG 1 cut(s) 279
SmlI CTYRAG 2 cut(s) 272, 279
SmoI CTYRAG 2 cut(s) 272, 279
Sse9I AATT 1 cut(s) 188
SspMI CTAG 1 cut(s) 251
SstI GAGCTC 1 cut(s) 249
StyD4I CCNGG 1 cut(s) 39
TaaI ACNGT 1 cut(s) 312
TaiI ACGT 1 cut(s) 165
TaqI TCGA 5 cut(s) 24, 51, 69, 117, 280
TasI AATT 1 cut(s) 188
TfiI GAWTC 1 cut(s) 219
TseFI GTSAC 1 cut(s) 293
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 1 cut(s) 300
XhoI CTCGAG 1 cut(s) 279
XmiI GTMKAC 1 cut(s) 117
XmnI GAANNNNTTC 1 cut(s) 75
XspI CTAG 1 cut(s) 251
ZraI GACGTC 1 cut(s) 163
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.