Rw7G014580

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001519
Physical Location & Seq
Forward (+)
14279822 .. 14281347
1526 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G014580.1

Sequence Viewer

Length: 384 bp
ATGTCCAAGCCGATCAATGGTCAGCTCCAGACCCGGCCCAAAACCGCTCCAGCCAATTTCGGTCCCGACACGCCGTCCGCGCCGCCCAAATCTGCAGCTACACGGCAGGTTGAGCGGTATGTTGATTATGCTCTTGATGTTCCCATGTACTGTGTTTATCGGAAAAATAAGTGTATCGACTGTACTGGAACGACCTTTCGGGACTTTATGGCAGGAAAGCTTCGTTGCCTTCCAGGTGAATTACCAACACTGAATGATTGGGAGAATCATTTGGGAACTATTTATCCAGAGGTCAGGCTGAAGAGATTCTTGGAGATGAGGGGTGCTGATGGAGGGCCTTGGAGAAGCTTATGCGCTTTGCCTGCATTTTGGGTAATGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

127

Amino Acids

14.31

Weight (kDa)

9.08

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 23 - 125 2.8e-17 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 97
AccBSI CCGCTC 2 cut(s) 47, 115
AccII CGCG 1 cut(s) 80
AciI CCGC 4 cut(s) 45, 78, 83, 115
AcuI CTGAAG 1 cut(s) 320
AfaI GTAC 2 cut(s) 149, 184
AfiI CCNNNNNNNGG 1 cut(s) 17
AhdI GACNNNNNGTC 1 cut(s) 73
AjnI CCWGG 1 cut(s) 232
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AloI GAACNNNNNNTCC 2 cut(s) 268, 300
AluBI AGCT 5 cut(s) 25, 98, 220, 348, 381
AluI AGCT 5 cut(s) 25, 98, 220, 348, 381
AoxI GGCC 2 cut(s) 35, 335
ApeKI GCWGC 1 cut(s) 95
Asp700I GAANNNNTTC 1 cut(s) 305
AspLEI GCGC 2 cut(s) 82, 356
AspS9I GGNCC 3 cut(s) 36, 62, 335
AsuC2I CCSGG 1 cut(s) 34
AsuHPI GGTGA 1 cut(s) 248
AvaII GGWCC 1 cut(s) 62
BbvI GCAGC 1 cut(s) 107
BccI CCATC 1 cut(s) 323
BceAI ACGGC 2 cut(s) 58, 119
BciT130I CCWGG 1 cut(s) 234
BcnI CCSGG 1 cut(s) 34
BfmI CTRYAG 1 cut(s) 93
BfuAI ACCTGC 1 cut(s) 97
BisI GCNGC 2 cut(s) 83, 96
BlsI GCNGC 2 cut(s) 84, 97
Bme1390I CCNGG 2 cut(s) 34, 234
Bme18I GGWCC 1 cut(s) 62
BmeRI GACNNNNNGTC 1 cut(s) 73
BmgT120I GGNCC 3 cut(s) 36, 62, 335
BmiI GGNNCC 1 cut(s) 64
BmrFI CCNGG 2 cut(s) 34, 234
BpmI CTGGAG 2 cut(s) 11, 33
BpuMI CCSGG 1 cut(s) 34
BsaJI CCNNGG 1 cut(s) 338
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse1I ACTGG 1 cut(s) 190
BseBI CCWGG 1 cut(s) 234
BseDI CCNNGG 1 cut(s) 338
BseLI CCNNNNNNNGG 1 cut(s) 17
BseNI ACTGG 1 cut(s) 190
BseXI GCAGC 1 cut(s) 107
Bsh1236I CGCG 1 cut(s) 80
BshFI GGCC 2 cut(s) 37, 337
BsiSI CCGG 1 cut(s) 34
BslFI GGGAC 2 cut(s) 48, 215
BslI CCNNNNNNNGG 1 cut(s) 17
BsmFI GGGAC 2 cut(s) 48, 215
BsnI GGCC 2 cut(s) 37, 337
Bsp143I GATC 1 cut(s) 12
BspACI CCGC 4 cut(s) 45, 78, 83, 115
BspANI GGCC 2 cut(s) 37, 337
BspFNI CGCG 1 cut(s) 80
BspLI GGNNCC 1 cut(s) 64
BspMAI CTGCAG 1 cut(s) 97
BspMI ACCTGC 1 cut(s) 97
BsrBI CCGCTC 2 cut(s) 47, 115
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 1 cut(s) 12
BssT1I CCWWGG 1 cut(s) 338
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 2 cut(s) 152, 182
Bst6I CTCTTC 1 cut(s) 296
BstC8I GCNNGC 1 cut(s) 363
BstFNI CGCG 1 cut(s) 80
BstHHI GCGC 2 cut(s) 82, 356
BstKTI GATC 1 cut(s) 15
BstMBI GATC 1 cut(s) 12
BstMWI GCNNNNNNNGC 3 cut(s) 79, 112, 362
BstNI CCWGG 1 cut(s) 234
BstSCI CCNGG 2 cut(s) 32, 232
BstSFI CTRYAG 1 cut(s) 93
BstUI CGCG 1 cut(s) 80
BstV1I GCAGC 1 cut(s) 107
BsuRI GGCC 2 cut(s) 37, 337
BtsIMutI CAGTG 1 cut(s) 248
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 1 cut(s) 363
CfoI GCGC 2 cut(s) 82, 356
Cfr13I GGNCC 3 cut(s) 36, 62, 335
Csp6I GTAC 2 cut(s) 148, 183
CviAII CATG 1 cut(s) 145
CviQI GTAC 2 cut(s) 148, 183
DpnI GATC 1 cut(s) 14
DpnII GATC 1 cut(s) 12
DriI GACNNNNNGTC 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 296
Eam1105I GACNNNNNGTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 296
Eco130I CCWWGG 1 cut(s) 338
Eco47I GGWCC 1 cut(s) 62
Eco57I CTGAAG 1 cut(s) 320
EcoO109I RGGNCCY 1 cut(s) 335
EcoRII CCWGG 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 338
ErhI CCWWGG 1 cut(s) 338
FaeI CATG 1 cut(s) 148
FaiI YATR 5 cut(s) 120, 129, 146, 209, 352
FalI AAGNNNNNCTT 2 cut(s) 293, 325
FaqI GGGAC 2 cut(s) 48, 215
FatI CATG 1 cut(s) 144
Fnu4HI GCNGC 2 cut(s) 83, 96
Fsp4HI GCNGC 2 cut(s) 83, 96
GlaI GCGC 2 cut(s) 81, 355
GluI GCNGC 2 cut(s) 83, 96
GsuI CTGGAG 2 cut(s) 11, 33
HaeIII GGCC 2 cut(s) 37, 337
HapII CCGG 1 cut(s) 34
HhaI GCGC 2 cut(s) 82, 356
Hin1II CATG 1 cut(s) 148
Hin6I GCGC 2 cut(s) 80, 354
HinP1I GCGC 2 cut(s) 80, 354
HindIII AAGCTT 2 cut(s) 218, 346
HinfI GANTC 2 cut(s) 265, 306
HpaII CCGG 1 cut(s) 34
HphI GGTGA 1 cut(s) 248
Hpy188I TCNGA 1 cut(s) 162
Hpy188III TCNNGA 5 cut(s) 28, 65, 134, 200, 287
HpyAV CCTTC 1 cut(s) 239
HpyCH4III ACNGT 2 cut(s) 152, 182
HpyCH4V TGCA 2 cut(s) 95, 365
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 112, 362
Hsp92II CATG 1 cut(s) 148
HspAI GCGC 2 cut(s) 80, 354
Kzo9I GATC 1 cut(s) 12
LmnI GCTCC 2 cut(s) 30, 52
Lsp1109I GCAGC 1 cut(s) 107
MalI GATC 1 cut(s) 14
MbiI CCGCTC 2 cut(s) 47, 115
MboI GATC 1 cut(s) 12
MboII GAAGA 1 cut(s) 313
MluCI AATT 2 cut(s) 55, 239
MnlI CCTC 3 cut(s) 283, 312, 326
MroXI GAANNNNTTC 1 cut(s) 305
MspI CCGG 1 cut(s) 34
MspR9I CCNGG 2 cut(s) 34, 234
MvaI CCWGG 1 cut(s) 234
MvnI CGCG 1 cut(s) 80
MwoI GCNNNNNNNGC 3 cut(s) 79, 112, 362
NciI CCSGG 1 cut(s) 34
NdeII GATC 1 cut(s) 12
NlaIII CATG 1 cut(s) 148
NlaIV GGNNCC 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 2 cut(s) 265, 306
PkrI GCNGC 2 cut(s) 84, 97
Psp6I CCWGG 1 cut(s) 232
PspGI CCWGG 1 cut(s) 232
PspN4I GGNNCC 1 cut(s) 64
PspPI GGNCC 3 cut(s) 36, 62, 335
PstI CTGCAG 1 cut(s) 97
RsaI GTAC 2 cut(s) 149, 184
RsaNI GTAC 2 cut(s) 148, 183
SatI GCNGC 2 cut(s) 83, 96
Sau3AI GATC 1 cut(s) 12
Sau96I GGNCC 3 cut(s) 36, 62, 335
ScrFI CCNGG 2 cut(s) 34, 234
SetI ASST 9 cut(s) 27, 100, 111, 197, 222, 238, 294, 350, 383
SfcI CTRYAG 1 cut(s) 93
SinI GGWCC 1 cut(s) 62
Sse9I AATT 2 cut(s) 55, 239
SsiI CCGC 4 cut(s) 45, 78, 83, 115
StyD4I CCNGG 2 cut(s) 32, 232
StyI CCWWGG 1 cut(s) 338
TaaI ACNGT 2 cut(s) 152, 182
TaqI TCGA 1 cut(s) 177
TaqII GACCGA 1 cut(s) 50
TasI AATT 2 cut(s) 55, 239
TatI WGTACW 2 cut(s) 147, 182
TauI GCSGC 1 cut(s) 85
TfiI GAWTC 2 cut(s) 265, 306
TscAI CASTG 1 cut(s) 255
TseI GCWGC 1 cut(s) 95
TspRI CASTG 1 cut(s) 255
VpaK11BI GGWCC 1 cut(s) 62
XmnI GAANNNNTTC 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.