Rorug07G0043100

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001519
Physical Location & Seq
Forward (+)
3096455 .. 3097683
1229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0043100.1

Sequence Viewer

Length: 954 bp
ATGGGAAAGCAATCTAACAGTACTGTTGCATCTCCTGGAATAAGGAGAGGTCCATGGACTCCTCAGGAGGACAAGAAGCTCTTTGCTTTCGTTCAACAACATGGCCATGGAAGCTGGCGTTCCTTGCCAGAGAAAGCTGGTCTCCAAAGATGTGGGAAGAGCTGCAGGCTAAGATGGAGAAACTACCTAAACCCTGATATTAAGAGAGGAAATTTCAGTTTGCAGGAAGACCAGACCATCATTCTACTTCATGCACTTTTGGGCAACAGGTGGTCGGCCATAGCTGCTAACTTACCGAGGAGAACAGACAATGAGATAAAGAACTACTGGAACACACATCTAAAGAAGAGGTTAGCCAAGAGAGGGTTTGATCCTGTAACCCACAAGCCCAAGACCGCGATCCATGCCTCTGCCAATGGCGACGACCCGAAGAACCTCTCAAACCTCAGCCACATGGCTCAGTGGGAAACTGCTCGGCTTCAAGCAGAAGGTAGATTTTCCAGACAGTCAAAACTACTCATCAGTCACGACCAACCTCCTAGATCTGGACCCGTGCCTGTACCACAATGGCTTGACATGAACGAAAGAGCACGGGAAATCCTGCTACTGTTGAAGTCAATATTAGCTGCAGACAGTGGTGGCCAAGGTGGTGGTATTGGTAGTACTTCCACCCACGTTGGGCAGGAGGTTGGTTCATTTGGTAAAGTTGATGTTGAAGGCGTACTTACTAATTGTGATCAGTTTGAGGCTCCAAGAACCAGTACATCAGTTCTGGGTTTTGATCAAGTTTTGGACGAAGTTTTGGGGGAGTCTGGTACTAATAATGAATATGATGATTTATTGGAGGCATCACTACTCCAGCACAATCATAACAGCACTGGATTTGAAGTTGGGGATGCTTGGAGTTTGGAACATTGTGCAACAGTAATGATGAAGGAGCAGGGAACAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

317

Amino Acids

35.15

Weight (kDa)

8.32

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 16 - 63 3.6e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 19 - 79 1.4e-13 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 69 - 114 2.8e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 72 - 123 3.7e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 398
AciI CCGC 1 cut(s) 396
AclWI GGATC 2 cut(s) 365, 394
AcoI YGGCCR 3 cut(s) 103, 276, 640
AcsI RAATTY 1 cut(s) 211
AfaI GTAC 6 cut(s) 22, 561, 664, 723, 763, 817
AfiI CCNNNNNNNGG 3 cut(s) 363, 545, 678
AgsI TTSAA 5 cut(s) 95, 482, 613, 716, 887
AjnI CCWGG 1 cut(s) 34
AluBI AGCT 6 cut(s) 79, 114, 137, 162, 284, 626
AluI AGCT 6 cut(s) 79, 114, 137, 162, 284, 626
Alw21I GWGCWC 1 cut(s) 592
Alw26I GTCTC 1 cut(s) 146
AlwI GGATC 2 cut(s) 365, 394
AoxI GGCC 3 cut(s) 103, 276, 640
ApeKI GCWGC 3 cut(s) 162, 284, 626
ApoI RAATTY 1 cut(s) 211
AspS9I GGNCC 2 cut(s) 50, 548
AvaII GGWCC 2 cut(s) 50, 548
AxyI CCTNAGG 1 cut(s) 63
BalI TGGCCA 2 cut(s) 105, 642
BbsI GAAGAC 1 cut(s) 234
Bbv12I GWGCWC 1 cut(s) 592
BbvCI CCTCAGC 1 cut(s) 446
BbvI GCAGC 3 cut(s) 149, 271, 613
BccI CCATC 2 cut(s) 168, 245
BciT130I CCWGG 1 cut(s) 36
BclI TGATCA 2 cut(s) 736, 781
BcoDI GTCTC 1 cut(s) 146
BfaI CTAG 1 cut(s) 540
BfmI CTRYAG 2 cut(s) 163, 627
BglII AGATCT 1 cut(s) 542
BisI GCNGC 3 cut(s) 163, 285, 627
BlsI GCNGC 3 cut(s) 164, 286, 628
BmcAI AGTACT 2 cut(s) 22, 664
Bme1390I CCNGG 1 cut(s) 36
Bme18I GGWCC 2 cut(s) 50, 548
BmgT120I GGNCC 2 cut(s) 50, 548
BmiI GGNNCC 2 cut(s) 550, 750
BmrFI CCNGG 1 cut(s) 36
BmsI GCATC 3 cut(s) 38, 857, 886
BpiI GAAGAC 1 cut(s) 234
BpmI CTGGAG 1 cut(s) 842
Bpu10I CCTNAGC 1 cut(s) 446
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 4 cut(s) 53, 106, 296, 643
Bsc4I CCNNNNNNNGG 3 cut(s) 363, 545, 678
Bse1I ACTGG 3 cut(s) 332, 759, 883
Bse21I CCTNAGG 1 cut(s) 63
BseBI CCWGG 1 cut(s) 36
BseDI CCNNGG 4 cut(s) 53, 106, 296, 643
BseGI GGATG 1 cut(s) 901
BseLI CCNNNNNNNGG 3 cut(s) 363, 545, 678
BseMII CTCAG 3 cut(s) 77, 460, 473
BseNI ACTGG 3 cut(s) 332, 759, 883
BseRI GAGGAG 2 cut(s) 51, 313
BseXI GCAGC 3 cut(s) 149, 271, 613
Bsh1236I CGCG 1 cut(s) 398
BshFI GGCC 3 cut(s) 105, 278, 642
BsiHKAI GWGCWC 1 cut(s) 592
BslI CCNNNNNNNGG 3 cut(s) 363, 545, 678
BsmAI GTCTC 1 cut(s) 146
BsnI GGCC 3 cut(s) 105, 278, 642
Bso31I GGTCTC 1 cut(s) 146
Bsp1286I GDGCHC 1 cut(s) 592
Bsp143I GATC 5 cut(s) 370, 399, 542, 736, 781
Bsp19I CCATGG 2 cut(s) 53, 106
BspACI CCGC 1 cut(s) 396
BspANI GGCC 3 cut(s) 105, 278, 642
BspCNI CTCAG 3 cut(s) 76, 459, 472
BspFNI CGCG 1 cut(s) 398
BspLI GGNNCC 2 cut(s) 550, 750
BspMAI CTGCAG 2 cut(s) 167, 631
BspPI GGATC 2 cut(s) 365, 394
BspQI GCTCTTC 1 cut(s) 152
BspTNI GGTCTC 1 cut(s) 146
BsrI ACTGG 3 cut(s) 332, 759, 883
BssECI CCNNGG 4 cut(s) 53, 106, 296, 643
BssMI GATC 5 cut(s) 370, 399, 542, 736, 781
BssT1I CCWWGG 3 cut(s) 53, 106, 643
Bst2UI CCWGG 1 cut(s) 36
Bst4CI ACNGT 6 cut(s) 20, 25, 507, 609, 635, 925
Bst6I CTCTTC 2 cut(s) 152, 341
BstC8I GCNNGC 2 cut(s) 116, 167
BstDEI CTNAG 4 cut(s) 63, 170, 446, 459
BstDSI CCRYGG 2 cut(s) 53, 106
BstF5I GGATG 1 cut(s) 901
BstFNI CGCG 1 cut(s) 398
BstKTI GATC 5 cut(s) 373, 402, 545, 739, 784
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 5 cut(s) 370, 399, 542, 736, 781
BstMWI GCNNNNNNNGC 4 cut(s) 111, 124, 284, 404
BstNI CCWGG 1 cut(s) 36
BstSCI CCNGG 1 cut(s) 34
BstSFI CTRYAG 2 cut(s) 163, 627
BstUI CGCG 1 cut(s) 398
BstV1I GCAGC 3 cut(s) 149, 271, 613
BstV2I GAAGAC 1 cut(s) 234
BstX2I RGATCY 1 cut(s) 542
BstXI CCANNNNNNTGG 2 cut(s) 152, 650
BstYI RGATCY 1 cut(s) 542
Bsu36I CCTNAGG 1 cut(s) 63
BsuRI GGCC 3 cut(s) 105, 278, 642
BtgI CCRYGG 2 cut(s) 53, 106
BtsCI GGATG 1 cut(s) 901
BtsIMutI CAGTG 3 cut(s) 467, 640, 876
Cac8I GCNNGC 2 cut(s) 116, 167
Cfr13I GGNCC 2 cut(s) 50, 548
Csp6I GTAC 6 cut(s) 21, 560, 663, 722, 762, 816
CspCI CAANNNNNGTGG 2 cut(s) 658, 693
CviAII CATG 7 cut(s) 54, 101, 107, 251, 404, 454, 577
CviQI GTAC 6 cut(s) 21, 560, 663, 722, 762, 816
DdeI CTNAG 4 cut(s) 63, 170, 446, 459
DpnI GATC 5 cut(s) 372, 401, 544, 738, 783
DpnII GATC 5 cut(s) 370, 399, 542, 736, 781
EaeI YGGCCR 3 cut(s) 103, 276, 640
Eam1104I CTCTTC 2 cut(s) 152, 341
EarI CTCTTC 2 cut(s) 152, 341
Eco130I CCWWGG 3 cut(s) 53, 106, 643
Eco31I GGTCTC 1 cut(s) 146
Eco47I GGWCC 2 cut(s) 50, 548
Eco81I CCTNAGG 1 cut(s) 63
EcoRII CCWGG 1 cut(s) 34
EcoT14I CCWWGG 3 cut(s) 53, 106, 643
ErhI CCWWGG 3 cut(s) 53, 106, 643
FaeI CATG 7 cut(s) 57, 104, 110, 254, 407, 457, 580
FalI AAGNNNNNCTT 4 cut(s) 65, 97, 708, 740
FatI CATG 7 cut(s) 53, 100, 106, 250, 403, 453, 576
FbaI TGATCA 2 cut(s) 736, 781
Fnu4HI GCNGC 3 cut(s) 163, 285, 627
FokI GGATG 1 cut(s) 908
Fsp4HI GCNGC 3 cut(s) 163, 285, 627
FspBI CTAG 1 cut(s) 540
GluI GCNGC 3 cut(s) 163, 285, 627
GsuI CTGGAG 1 cut(s) 842
HaeIII GGCC 3 cut(s) 105, 278, 642
Hin1II CATG 7 cut(s) 57, 104, 110, 254, 407, 457, 580
HinfI GANTC 2 cut(s) 58, 809
Hpy188III TCNNGA 4 cut(s) 65, 501, 527, 546
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 3 cut(s) 482, 710, 928
HpyCH4III ACNGT 6 cut(s) 20, 25, 507, 609, 635, 925
HpyCH4IV ACGT 1 cut(s) 675
HpyCH4V TGCA 6 cut(s) 29, 165, 223, 254, 629, 920
HpyF10VI GCNNNNNNNGC 4 cut(s) 111, 124, 284, 404
HpyF3I CTNAG 4 cut(s) 63, 170, 446, 459
HpySE526I ACGT 1 cut(s) 675
Hsp92II CATG 7 cut(s) 57, 104, 110, 254, 407, 457, 580
Ksp22I TGATCA 2 cut(s) 736, 781
Kzo9I GATC 5 cut(s) 370, 399, 542, 736, 781
LguI GCTCTTC 1 cut(s) 152
LmnI GCTCC 2 cut(s) 754, 937
Lsp1109I GCAGC 3 cut(s) 149, 271, 613
LweI GCATC 3 cut(s) 38, 857, 886
MaeI CTAG 1 cut(s) 540
MaeII ACGT 1 cut(s) 675
MaeIII GTNAC 2 cut(s) 376, 524
MalI GATC 5 cut(s) 372, 401, 544, 738, 783
MboI GATC 5 cut(s) 370, 399, 542, 736, 781
MboII GAAGA 4 cut(s) 169, 239, 358, 442
MflI RGATCY 1 cut(s) 542
MhlI GDGCHC 1 cut(s) 592
MlsI TGGCCA 2 cut(s) 105, 642
MluCI AATT 3 cut(s) 211, 730, 949
MluNI TGGCCA 2 cut(s) 105, 642
MlyI GAGTC 2 cut(s) 52, 818
Mox20I TGGCCA 2 cut(s) 105, 642
MscI TGGCCA 2 cut(s) 105, 642
MseI TTAA 2 cut(s) 201, 952
MslI CAYNNNNRTG 1 cut(s) 105
Msp20I TGGCCA 2 cut(s) 105, 642
MspR9I CCNGG 1 cut(s) 36
MvaI CCWGG 1 cut(s) 36
MvnI CGCG 1 cut(s) 398
MwoI GCNNNNNNNGC 4 cut(s) 111, 124, 284, 404
NcoI CCATGG 2 cut(s) 53, 106
NdeII GATC 5 cut(s) 370, 399, 542, 736, 781
NlaIII CATG 7 cut(s) 57, 104, 110, 254, 407, 457, 580
NlaIV GGNNCC 2 cut(s) 550, 750
NmeAIII GCCGAG 1 cut(s) 454
NmuCI GTSAC 1 cut(s) 524
PciSI GCTCTTC 1 cut(s) 152
PfoI TCCNGGA 1 cut(s) 34
PkrI GCNGC 3 cut(s) 164, 286, 628
PleI GAGTC 2 cut(s) 52, 817
PpsI GAGTC 2 cut(s) 52, 817
Psp6I CCWGG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 34
PspN4I GGNNCC 2 cut(s) 550, 750
PspPI GGNCC 2 cut(s) 50, 548
PstI CTGCAG 2 cut(s) 167, 631
PsuI RGATCY 1 cut(s) 542
RsaI GTAC 6 cut(s) 22, 561, 664, 723, 763, 817
RsaNI GTAC 6 cut(s) 21, 560, 663, 722, 762, 816
RseI CAYNNNNRTG 1 cut(s) 105
SapI GCTCTTC 1 cut(s) 152
SaqAI TTAA 2 cut(s) 201, 952
SatI GCNGC 3 cut(s) 163, 285, 627
Sau3AI GATC 5 cut(s) 370, 399, 542, 736, 781
Sau96I GGNCC 2 cut(s) 50, 548
ScaI AGTACT 2 cut(s) 22, 664
SchI GAGTC 2 cut(s) 52, 818
ScrFI CCNGG 1 cut(s) 36
SduI GDGCHC 1 cut(s) 592
SfaNI GCATC 3 cut(s) 38, 857, 886
SfcI CTRYAG 2 cut(s) 163, 627
SinI GGWCC 2 cut(s) 50, 548
SmiMI CAYNNNNRTG 1 cut(s) 105
Sse9I AATT 3 cut(s) 211, 730, 949
SsiI CCGC 1 cut(s) 396
SspI AATATT 1 cut(s) 621
SspMI CTAG 1 cut(s) 540
StyD4I CCNGG 1 cut(s) 34
StyI CCWWGG 3 cut(s) 53, 106, 643
TaaI ACNGT 6 cut(s) 20, 25, 507, 609, 635, 925
TaiI ACGT 1 cut(s) 678
TasI AATT 3 cut(s) 211, 730, 949
TatI WGTACW 3 cut(s) 20, 662, 761
Tru1I TTAA 2 cut(s) 201, 952
Tru9I TTAA 2 cut(s) 201, 952
TscAI CASTG 3 cut(s) 467, 640, 883
TseFI GTSAC 1 cut(s) 524
TseI GCWGC 3 cut(s) 162, 284, 626
Tsp45I GTSAC 1 cut(s) 524
TspDTI ATGAA 5 cut(s) 239, 593, 684, 840, 947
TspRI CASTG 3 cut(s) 467, 640, 883
VpaK11BI GGWCC 2 cut(s) 50, 548
XapI RAATTY 1 cut(s) 211
XspI CTAG 1 cut(s) 540
ZrmI AGTACT 2 cut(s) 22, 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.