MD06G1082500.v1.1

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001519
Physical Location & Seq
Reverse (-)
20279496 .. 20285942
6447 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1082500.v1.1.491

Sequence Viewer

Length: 1581 bp
ATGGTACTCATTTCACAGACGGGTTCATCATACTGCATCCGTACTGAGCTATTTCGGTATAAATCTGGACAAAATGTGGTTTTCAGTAGGGCAAACAATATAGAGGCGTCCAAGTTGAAGGAAAATTCCATTGGCTTCTCTTCTGTATCATATTCTTCTTCTAGGAAGACACAGATTTTGCATATAGACAATGCACGAGTAGGACAGAAACGAGGGCATAGTGTGATAGTTGCTGCCAGTCCTCCAACAGAAGATGCAGTAATTGCTACTGAGCCACTAACGAAAGAGGATCTTGTAGCGTATCTTGCCTCTGGATGCAAGTCTAAGGAAAAATGGAGAATTGGCACAGAGCACGAGAAATTTGGTTTTGAGCTAAAGACCTTACGCCCTATAAAATATGAACAAATAGCCGAGTTGCTTAATGTTATTTCTGAGAGATTTGATTGGGAAAAAGTTATGGAAGGCGACAAAATTATTGGACTCAAACAGGGTAAGCAAAGCATATCACTGGAGCCTGGAGGTCAATTTGAACTTAGTGGTGCACCTCTTGCGACACTGCATCAAACTTGTGCTGAAGTCAATTCACACCTTTATCAGGTCAAAGCAATTACAGAGGAAATGGGAATCGGATTTCTTGGAATCGGTTTTCAGCCCAAATGGGGACTTAAGGACATACCTATTATGCCCAAGGGAAGATATGATATTATGAGAAATTACATGCCAACAGTTGGCACCCTTGGACTTGATATGATGTTCAGAACTTGCACTGTACAGGTTAATCTGGACTTCAGTTCTGAAGCAGACATGATAAGGAAATTTCGTGCTGGTCTTGCCTTGCAGCCTATAGCAACAGCTCTATTCGCAAATTCACCTTTCACTGAAGGAAAGCCAAATGGTTTTCTAAGCATGAGAAGCCAAATTTGGACTGATATGGACAATAATCGCACTGGCATGCTTCCTTTTGTTTTTGATGACTCTTTTGGGTTTGAGCAGTATGTAGATTATGCTCTTGATGTTCCCATGTACTTTGTTTATCGGAACAAGAAGTATATCGATTGCACTGGAATGACCTTCCGGGACTTTTTGGTAGGGAAGCTTCCTTGCATTCCGGGTGAATTGCCAACCCTGAACGATTGGGAGAATCATTTGACAACTATATTCCCTGAGGTCAGGCTAAAAAGATACTTGGAGATGAGGGGTGCTGATGGAGGGCCTTGGAGGAGATTATGTGCTTTGCCCGCATTTTGGGTAGGTATTTTGTACGACGAGGTTTCCTTACAAAACGTGTTAGACCTTACAGCTGATTGGACCCCTGAAGAAAGACAGATGCTGAGAAATAAGGTCCCCATAACTGGTCTAAAGACGCCATTTCGGGATGGATTGCTCAAGCATGTAGCTCAAGATGTTGTAAAGTTGGCAAGGGATGGCCTAGAAAGACGAGGCTTCAAAGAAACAGGATTCTTGAATGAGGTGGCGGAGGTGGTTAGAACTGGTGTAACACCAGCTGAGAAGCTTTTGGAATTGTACAATGGGAAATGGGGACAACAGATAGACCCTGTTTTCGAGGAGCTACTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

527

Amino Acids

59.55

Weight (kDa)

6.45

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 113 - 522 1.4e-104 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 731
AccB7I CCANNNNNTGG 1 cut(s) 728
AciI CCGC 2 cut(s) 1239, 1475
AclWI GGATC 1 cut(s) 297
AcsI RAATTY 5 cut(s) 124, 359, 815, 865, 918
AcuI CTGAAG 5 cut(s) 594, 772, 816, 900, 1335
AcyI GRCGYC 2 cut(s) 107, 1364
AfaI GTAC 6 cut(s) 6, 43, 771, 1025, 1262, 1526
AfiI CCNNNNNNNGG 5 cut(s) 595, 659, 728, 1245, 1352
AflII CTTAAG 1 cut(s) 665
AflIII ACRYGT 1 cut(s) 1284
AgsI TTSAA 4 cut(s) 118, 530, 1447, 1465
AjnI CCWGG 1 cut(s) 514
AjuI GAANNNNNNNTTGG 4 cut(s) 114, 146, 904, 936
AluBI AGCT 9 cut(s) 49, 373, 854, 1096, 1301, 1397, 1505, 1513, 1570
AluI AGCT 9 cut(s) 49, 373, 854, 1096, 1301, 1397, 1505, 1513, 1570
Alw21I GWGCWC 2 cut(s) 354, 544
Alw44I GTGCAC 1 cut(s) 540
AlwI GGATC 1 cut(s) 297
AlwNI CAGNNNCTG 1 cut(s) 1330
AoxI GGCC 2 cut(s) 1211, 1426
ApaLI GTGCAC 1 cut(s) 540
ApeKI GCWGC 2 cut(s) 233, 838
ApoI RAATTY 5 cut(s) 124, 359, 815, 865, 918
ArsI GACNNNNNNTTYG 4 cut(s) 160, 192, 1544, 1576
AspS9I GGNCC 3 cut(s) 1211, 1308, 1342
AsuC2I CCSGG 2 cut(s) 1076, 1110
AsuHPI GGTGA 2 cut(s) 861, 1124
AvaII GGWCC 2 cut(s) 1308, 1342
AxyI CCTNAGG 1 cut(s) 1164
BaeGI GKGCMC 1 cut(s) 544
BanI GGYRCC 1 cut(s) 731
BarI GAAGNNNNNNTAC 2 cut(s) 1080, 1112
BauI CACGAG 2 cut(s) 195, 353
BbsI GAAGAC 1 cut(s) 173
Bbv12I GWGCWC 2 cut(s) 354, 544
BbvI GCAGC 2 cut(s) 220, 850
BccI CCATC 3 cut(s) 1199, 1370, 1418
BciT130I CCWGG 1 cut(s) 516
BcnI CCSGG 2 cut(s) 1076, 1110
BfaI CTAG 2 cut(s) 162, 1430
BfmI CTRYAG 1 cut(s) 843
BfrI CTTAAG 1 cut(s) 665
BisI GCNGC 2 cut(s) 234, 839
BlsI GCNGC 2 cut(s) 235, 840
Bme1390I CCNGG 3 cut(s) 516, 1076, 1110
Bme18I GGWCC 2 cut(s) 1308, 1342
BmgT120I GGNCC 3 cut(s) 1211, 1308, 1342
BmiI GGNNCC 4 cut(s) 513, 733, 1310, 1344
BmrFI CCNGG 3 cut(s) 516, 1076, 1110
BmsI GCATC 5 cut(s) 45, 244, 305, 568, 1317
BpiI GAAGAC 1 cut(s) 173
BpmI CTGGAG 2 cut(s) 530, 537
BpuEI CTTGAG 2 cut(s) 1370, 1383
BpuMI CCSGG 2 cut(s) 1076, 1110
Bsa29I ATCGAT 1 cut(s) 1053
BsaHI GRCGYC 2 cut(s) 107, 1364
BsaJI CCNNGG 3 cut(s) 687, 736, 1214
BsaXI ACNNNNNCTCC 2 cut(s) 1213, 1243
Bsc4I CCNNNNNNNGG 5 cut(s) 595, 659, 728, 1245, 1352
Bse1I ACTGG 6 cut(s) 237, 513, 952, 1066, 1357, 1495
Bse21I CCTNAGG 1 cut(s) 1164
BseBI CCWGG 1 cut(s) 516
BseCI ATCGAT 1 cut(s) 1053
BseDI CCNNGG 3 cut(s) 687, 736, 1214
BseGI GGATG 4 cut(s) 36, 320, 1381, 1429
BseLI CCNNNNNNNGG 5 cut(s) 595, 659, 728, 1245, 1352
BseMII CTCAG 6 cut(s) 36, 261, 423, 1155, 1322, 1497
BseNI ACTGG 6 cut(s) 237, 513, 952, 1066, 1357, 1495
BseRI GAGGAG 2 cut(s) 1234, 1580
BseSI GKGCMC 1 cut(s) 544
BseXI GCAGC 2 cut(s) 220, 850
BshFI GGCC 2 cut(s) 1213, 1428
BshNI GGYRCC 1 cut(s) 731
BshVI ATCGAT 1 cut(s) 1053
BsiHKAI GWGCWC 2 cut(s) 354, 544
BsiSI CCGG 2 cut(s) 1075, 1109
BslFI GGGAC 4 cut(s) 675, 1091, 1328, 1554
BslI CCNNNNNNNGG 5 cut(s) 595, 659, 728, 1245, 1352
BsmFI GGGAC 4 cut(s) 675, 1091, 1328, 1554
BsmI GAATGC 1 cut(s) 1104
BsnI GGCC 2 cut(s) 1213, 1428
Bsp1286I GDGCHC 2 cut(s) 354, 544
Bsp1407I TGTACA 2 cut(s) 769, 1524
Bsp143I GATC 1 cut(s) 289
BspACI CCGC 2 cut(s) 1239, 1475
BspANI GGCC 2 cut(s) 1213, 1428
BspCNI CTCAG 6 cut(s) 37, 262, 424, 1156, 1323, 1498
BspDI ATCGAT 1 cut(s) 1053
BspLI GGNNCC 4 cut(s) 513, 733, 1310, 1344
BspPI GGATC 1 cut(s) 297
BspT107I GGYRCC 1 cut(s) 731
BspTI CTTAAG 1 cut(s) 665
BsrGI TGTACA 2 cut(s) 769, 1524
BsrI ACTGG 6 cut(s) 237, 513, 952, 1066, 1357, 1495
BssECI CCNNGG 3 cut(s) 687, 736, 1214
BssMI GATC 1 cut(s) 289
BssNI GRCGYC 2 cut(s) 107, 1364
BssSI CACGAG 2 cut(s) 195, 353
BssT1I CCWWGG 3 cut(s) 687, 736, 1214
Bst2BI CACGAG 2 cut(s) 195, 353
Bst2UI CCWGG 1 cut(s) 516
Bst4CI ACNGT 2 cut(s) 727, 769
Bst6I CTCTTC 1 cut(s) 145
BstACI GRCGYC 2 cut(s) 107, 1364
BstAFI CTTAAG 1 cut(s) 665
BstAPI GCANNNNNTGC 2 cut(s) 263, 548
BstAUI TGTACA 2 cut(s) 769, 1524
BstC8I GCNNGC 2 cut(s) 953, 1239
BstDEI CTNAG 9 cut(s) 45, 270, 324, 432, 533, 902, 1164, 1331, 1506
BstENI CCTNNNNNAGG 1 cut(s) 593
BstF5I GGATG 4 cut(s) 36, 320, 1381, 1429
BstKTI GATC 1 cut(s) 292
BstMBI GATC 1 cut(s) 289
BstMWI GCNNNNNNNGC 7 cut(s) 263, 305, 548, 830, 860, 912, 1238
BstNI CCWGG 1 cut(s) 516
BstNSI RCATGY 3 cut(s) 721, 955, 1394
BstSCI CCNGG 3 cut(s) 514, 1074, 1108
BstSFI CTRYAG 1 cut(s) 843
BstSLI GKGCMC 1 cut(s) 544
BstV1I GCAGC 2 cut(s) 220, 850
BstV2I GAAGAC 1 cut(s) 173
BstX2I RGATCY 1 cut(s) 289
BstYI RGATCY 1 cut(s) 289
Bsu15I ATCGAT 1 cut(s) 1053
Bsu36I CCTNAGG 1 cut(s) 1164
BsuRI GGCC 2 cut(s) 1213, 1428
BsuTUI ATCGAT 1 cut(s) 1053
BtsCI GGATG 4 cut(s) 36, 320, 1381, 1429
BtsI GCAGTG 1 cut(s) 554
BtsIMutI CAGTG 6 cut(s) 506, 554, 765, 876, 945, 1059
Cac8I GCNNGC 2 cut(s) 953, 1239
CaiI CAGNNNCTG 1 cut(s) 1330
Cfr13I GGNCC 3 cut(s) 1211, 1308, 1342
ClaI ATCGAT 1 cut(s) 1053
CseI GACGC 2 cut(s) 96, 1372
Csp6I GTAC 6 cut(s) 5, 42, 770, 1024, 1261, 1525
CviAII CATG 6 cut(s) 718, 805, 907, 952, 1021, 1391
CviQI GTAC 6 cut(s) 5, 42, 770, 1024, 1261, 1525
DdeI CTNAG 9 cut(s) 45, 270, 324, 432, 533, 902, 1164, 1331, 1506
DpnI GATC 1 cut(s) 291
DpnII GATC 1 cut(s) 289
Eam1104I CTCTTC 1 cut(s) 145
EarI CTCTTC 1 cut(s) 145
EciI GGCGGA 1 cut(s) 1490
Eco130I CCWWGG 3 cut(s) 687, 736, 1214
Eco47I GGWCC 2 cut(s) 1308, 1342
Eco57I CTGAAG 5 cut(s) 594, 772, 816, 900, 1335
Eco81I CCTNAGG 1 cut(s) 1164
EcoNI CCTNNNNNAGG 1 cut(s) 593
EcoO109I RGGNCCY 2 cut(s) 1211, 1342
EcoRII CCWGG 1 cut(s) 514
EcoT14I CCWWGG 3 cut(s) 687, 736, 1214
ErhI CCWWGG 3 cut(s) 687, 736, 1214
FaeI CATG 6 cut(s) 721, 808, 910, 955, 1024, 1394
FalI AAGNNNNNCTT 2 cut(s) 276, 308
FaqI GGGAC 4 cut(s) 675, 1091, 1328, 1554
FatI CATG 6 cut(s) 717, 804, 906, 951, 1020, 1390
FauI CCCGC 1 cut(s) 1246
Fnu4HI GCNGC 2 cut(s) 234, 839
FokI GGATG 4 cut(s) 23, 327, 1388, 1436
Fsp4HI GCNGC 2 cut(s) 234, 839
FspBI CTAG 2 cut(s) 162, 1430
GluI GCNGC 2 cut(s) 234, 839
GsuI CTGGAG 2 cut(s) 530, 537
HaeIII GGCC 2 cut(s) 1213, 1428
HapII CCGG 2 cut(s) 1075, 1109
HgaI GACGC 2 cut(s) 96, 1372
Hin1I GRCGYC 2 cut(s) 107, 1364
Hin1II CATG 6 cut(s) 721, 808, 910, 955, 1024, 1394
HindIII AAGCTT 2 cut(s) 1094, 1511
HinfI GANTC 6 cut(s) 480, 624, 639, 974, 1141, 1458
HpaII CCGG 2 cut(s) 1075, 1109
HphI GGTGA 2 cut(s) 861, 1124
Hpy166II GTNNAC 1 cut(s) 542
Hpy188I TCNGA 5 cut(s) 433, 629, 758, 796, 1038
Hpy188III TCNNGA 7 cut(s) 66, 312, 782, 1010, 1373, 1400, 1462
Hpy8I GTNNAC 1 cut(s) 542
Hpy99I CGWCG 1 cut(s) 1268
HpyAV CCTTC 4 cut(s) 112, 455, 875, 1081
HpyCH4III ACNGT 2 cut(s) 727, 769
HpyCH4IV ACGT 1 cut(s) 1284
HpyF10VI GCNNNNNNNGC 7 cut(s) 263, 305, 548, 830, 860, 912, 1238
HpyF3I CTNAG 9 cut(s) 45, 270, 324, 432, 533, 902, 1164, 1331, 1506
HpySE526I ACGT 1 cut(s) 1284
Hsp92I GRCGYC 2 cut(s) 107, 1364
Hsp92II CATG 6 cut(s) 721, 808, 910, 955, 1024, 1394
Kzo9I GATC 1 cut(s) 289
LmnI GCTCC 2 cut(s) 511, 1567
Lsp1109I GCAGC 2 cut(s) 220, 850
LweI GCATC 5 cut(s) 45, 244, 305, 568, 1317
MaeI CTAG 2 cut(s) 162, 1430
MaeII ACGT 1 cut(s) 1284
MaeIII GTNAC 1 cut(s) 1495
MalI GATC 1 cut(s) 291
MboI GATC 1 cut(s) 289
MboII GAAGA 7 cut(s) 132, 147, 150, 178, 263, 705, 1328
MflI RGATCY 1 cut(s) 289
MhlI GDGCHC 2 cut(s) 354, 544
MlyI GAGTC 2 cut(s) 474, 968
MmeI TCCRAC 1 cut(s) 269
MseI TTAA 3 cut(s) 420, 666, 777
MslI CAYNNNNRTG 2 cut(s) 950, 1064
MspA1I CMGCKG 2 cut(s) 1301, 1505
MspCI CTTAAG 1 cut(s) 665
MspI CCGG 2 cut(s) 1075, 1109
MspR9I CCNGG 3 cut(s) 516, 1076, 1110
Mva1269I GAATGC 1 cut(s) 1104
MvaI CCWGG 1 cut(s) 516
MwoI GCNNNNNNNGC 7 cut(s) 263, 305, 548, 830, 860, 912, 1238
NciI CCSGG 2 cut(s) 1076, 1110
NdeII GATC 1 cut(s) 289
NlaIII CATG 6 cut(s) 721, 808, 910, 955, 1024, 1394
NlaIV GGNNCC 4 cut(s) 513, 733, 1310, 1344
NmeAIII GCCGAG 1 cut(s) 436
NspI RCATGY 3 cut(s) 721, 955, 1394
PaeI GCATGC 1 cut(s) 955
PctI GAATGC 1 cut(s) 1104
PfeI GAWTC 4 cut(s) 624, 639, 1141, 1458
PflMI CCANNNNNTGG 1 cut(s) 728
PfoI TCCNGGA 1 cut(s) 1074
PkrI GCNGC 2 cut(s) 235, 840
PleI GAGTC 2 cut(s) 474, 968
PpsI GAGTC 2 cut(s) 474, 968
PpuMI RGGWCCY 1 cut(s) 1342
Psp5II RGGWCCY 1 cut(s) 1342
Psp6I CCWGG 1 cut(s) 514
PspGI CCWGG 1 cut(s) 514
PspN4I GGNNCC 4 cut(s) 513, 733, 1310, 1344
PspPI GGNCC 3 cut(s) 1211, 1308, 1342
PspPPI RGGWCCY 1 cut(s) 1342
PstNI CAGNNNCTG 1 cut(s) 1330
PsuI RGATCY 1 cut(s) 289
PvuII CAGCTG 2 cut(s) 1301, 1505
RsaI GTAC 6 cut(s) 6, 43, 771, 1025, 1262, 1526
RsaNI GTAC 6 cut(s) 5, 42, 770, 1024, 1261, 1525
RseI CAYNNNNRTG 2 cut(s) 950, 1064
SaqAI TTAA 3 cut(s) 420, 666, 777
SatI GCNGC 2 cut(s) 234, 839
Sau3AI GATC 1 cut(s) 289
Sau96I GGNCC 3 cut(s) 1211, 1308, 1342
SchI GAGTC 2 cut(s) 474, 968
ScrFI CCNGG 3 cut(s) 516, 1076, 1110
SduI GDGCHC 2 cut(s) 354, 544
SfaNI GCATC 5 cut(s) 45, 244, 305, 568, 1317
SfcI CTRYAG 1 cut(s) 843
SinI GGWCC 2 cut(s) 1308, 1342
SmiMI CAYNNNNRTG 2 cut(s) 950, 1064
SmlI CTYRAG 3 cut(s) 665, 1385, 1398
SmoI CTYRAG 3 cut(s) 665, 1385, 1398
SphI GCATGC 1 cut(s) 955
SsiI CCGC 2 cut(s) 1239, 1475
SspMI CTAG 2 cut(s) 162, 1430
StyD4I CCNGG 3 cut(s) 514, 1074, 1108
StyI CCWWGG 3 cut(s) 687, 736, 1214
TaaI ACNGT 2 cut(s) 727, 769
TaiI ACGT 1 cut(s) 1287
TaqI TCGA 2 cut(s) 1053, 1563
TatI WGTACW 3 cut(s) 769, 1023, 1524
TfiI GAWTC 4 cut(s) 624, 639, 1141, 1458
Tru1I TTAA 3 cut(s) 420, 666, 777
Tru9I TTAA 3 cut(s) 420, 666, 777
TscAI CASTG 6 cut(s) 513, 561, 772, 883, 952, 1066
TseI GCWGC 2 cut(s) 233, 838
TspDTI ATGAA 2 cut(s) 15, 414
TspGWI ACGGA 1 cut(s) 29
TspRI CASTG 6 cut(s) 513, 561, 772, 883, 952, 1066
Van91I CCANNNNNTGG 1 cut(s) 728
Vha464I CTTAAG 1 cut(s) 665
VneI GTGCAC 1 cut(s) 540
VpaK11BI GGWCC 2 cut(s) 1308, 1342
XagI CCTNNNNNAGG 1 cut(s) 593
XapI RAATTY 5 cut(s) 124, 359, 815, 865, 918
XceI RCATGY 3 cut(s) 721, 955, 1394
XspI CTAG 2 cut(s) 162, 1430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.