Rw1G008510

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001519
Physical Location & Seq
Forward (+)
18407849 .. 18411025
3177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G008510.1

Sequence Viewer

Length: 1344 bp
ATGGCACTCATTTCCCAGACAAGACAGAAACAAGGACGTAAAAGTGCGATAGTTGCTTCAAGTCCTATCACAGAAGATGCTGTAGTAGCTACAACTCCGCTGACGAAAGAAGATCTTGTAGGCCATCTTGCCTCTGGATGCAAGCCTAAAGAAAAATGGAGAATTGGCATGGAACATGAGAGGTTTGGCTTCGATTTAAAAAGTTTACGTCCCATGCAATATCAACAAATAGCTGAACTGCTAAATGGGATTGCTGAGAGATATGAGTGGGAGAAATCATGGAAGGGCAAGCAGAGTATATCACTTGAGCCTGGAGGTCAGTTTGAGCTTAGTGGTGCACCTCTTGAAACTCTGCATCAAGTTTGTATGGAAGTTAAAGCAGTTGCAGAGAAAATGGGAATTGGATTTCTTGAAATTGGTGGGCAGCCGAAATGGAGCATTCAAGACATACCTATGATGCCCAGGGAATTATATGAGATTGCTGATAATTACATGCCAAAAGTTGGCTCGCTTGGACGTGATTTGATGGTCAGGACTTGCACAGTACAGGTTAATCTGGACTTCAGTTCTGAAGCAGACATGAAATGCAAAATTTGTGTTGGTCTTGCTTTGCAGCCTATAGCAACAGCCCTGTTTGCAAATTCTCCTTTTACTGAACGAAAGCCAAATGGTTTTTTAAGCAGGAGAAGTCACCTCTATACTGACACTGACAAGAATCGCACTGGCATGCTTCCTTTTGCCTTTGATAACTCCTTTGGGTTTGAGCGGTATGTTGATTATGCTCTTGTTCCCATGTACTATGTTTATCCGAAAAAGAAGTGTATCGACTGTTCTGGAGTGACCTTTCGGGACTTTATGGCTGGAAAGCTTCATTTCCTTCCAGGTGAATTACCGATCCTGAATGATTGGGAAAATCATTTGGGAACTCTATATCCAGAGGTCAGGCTGAAGAGATACTTAGAGATGAGGGGTGCTGATCGATGGAGGGCCTTGGAGGAGCTTATGTGCTTTGTGGGCTTACTGTATGATGAGGTTTCCCTGCATAATGTGTTAGACATGATAGCTGATTGGACTCCTGAAGCAAGACAGATGCTGAGAGATAAGGTTCGGAAAATGGGTCTTAAGACACCATTCCATGATGGATTACTCAAGCATGTTGCCGAAGATGTAGTAAAGTTTGCAAAGGATGGCCTGGAAAGAAGAGGCTTTAAGGAATTGGGGTTCTTAAATGAAGCGGTCGAGGTTGTGAGAACAGGAGTAACACCAGCTGAGAAGCTATTGGAGTTGCATAATGGGAAGTGGAAACAACAGATAGACCCAGTTTTTGAAGAGCTACTATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

447

Amino Acids

50.97

Weight (kDa)

6.58

Isoelectric Point (pI)

34.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 54 - 443 3e-75 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 503
AccBSI CCGCTC 1 cut(s) 766
AciI CCGC 3 cut(s) 98, 766, 1235
AclWI GGATC 1 cut(s) 889
AcsI RAATTY 2 cut(s) 591, 640
AcuI CTGAAG 4 cut(s) 547, 591, 968, 1098
AfaI GTAC 2 cut(s) 546, 797
AfiI CCNNNNNNNGG 1 cut(s) 503
AflII CTTAAG 1 cut(s) 1121
AgsI TTSAA 5 cut(s) 60, 347, 413, 443, 1328
AjiI CACGTC 1 cut(s) 518
AjnI CCWGG 4 cut(s) 310, 461, 880, 1191
AloI GAACNNNNNNTCC 4 cut(s) 916, 948, 1205, 1237
AluBI AGCT 9 cut(s) 89, 233, 328, 868, 1000, 1064, 1268, 1276, 1333
AluI AGCT 9 cut(s) 89, 233, 328, 868, 1000, 1064, 1268, 1276, 1333
Alw21I GWGCWC 1 cut(s) 340
Alw44I GTGCAC 1 cut(s) 336
AlwI GGATC 1 cut(s) 889
AlwNI CAGNNNCTG 1 cut(s) 1093
AoxI GGCC 3 cut(s) 121, 987, 1189
ApaLI GTGCAC 1 cut(s) 336
ApeKI GCWGC 2 cut(s) 424, 613
ApoI RAATTY 2 cut(s) 591, 640
ArsI GACNNNNNNTTYG 2 cut(s) 1307, 1339
AspS9I GGNCC 1 cut(s) 987
AsuHPI GGTGA 2 cut(s) 683, 896
BaeGI GKGCMC 1 cut(s) 340
Bbv12I GWGCWC 1 cut(s) 340
BbvI GCAGC 2 cut(s) 436, 625
BccI CCATC 5 cut(s) 132, 520, 975, 1133, 1181
BciT130I CCWGG 4 cut(s) 312, 463, 882, 1193
BfmI CTRYAG 2 cut(s) 81, 618
BfrI CTTAAG 1 cut(s) 1121
BglII AGATCT 1 cut(s) 112
BisI GCNGC 2 cut(s) 425, 614
BlsI GCNGC 2 cut(s) 426, 615
Bme1390I CCNGG 4 cut(s) 312, 463, 882, 1193
BmgBI CACGTC 1 cut(s) 518
BmgT120I GGNCC 1 cut(s) 987
BmrFI CCNGG 4 cut(s) 312, 463, 882, 1193
BmrI ACTGGG 1 cut(s) 1313
BmsI GCATC 5 cut(s) 67, 128, 364, 447, 1080
BmuI ACTGGG 1 cut(s) 1313
BpmI CTGGAG 2 cut(s) 333, 855
BpuEI CTTGAG 2 cut(s) 326, 1133
Bsa29I ATCGAT 1 cut(s) 979
BsaJI CCNNGG 3 cut(s) 461, 462, 990
BsaXI ACNNNNNCTCC 2 cut(s) 989, 1019
Bsc4I CCNNNNNNNGG 1 cut(s) 503
Bse1I ACTGG 2 cut(s) 727, 1319
BseBI CCWGG 4 cut(s) 312, 463, 882, 1193
BseCI ATCGAT 1 cut(s) 979
BseDI CCNNGG 3 cut(s) 461, 462, 990
BseGI GGATG 2 cut(s) 143, 1192
BseLI CCNNNNNNNGG 1 cut(s) 503
BseMII CTCAG 3 cut(s) 246, 1085, 1260
BseNI ACTGG 2 cut(s) 727, 1319
BseRI GAGGAG 1 cut(s) 1010
BseSI GKGCMC 1 cut(s) 340
BseXI GCAGC 2 cut(s) 436, 625
Bsh1285I CGRYCG 1 cut(s) 1239
BshFI GGCC 3 cut(s) 123, 989, 1191
BshVI ATCGAT 1 cut(s) 979
BsiEI CGRYCG 1 cut(s) 1239
BsiHKAI GWGCWC 1 cut(s) 340
BslFI GGGAC 2 cut(s) 195, 863
BslI CCNNNNNNNGG 1 cut(s) 503
BsmFI GGGAC 2 cut(s) 195, 863
BsmI GAATGC 1 cut(s) 438
BsnI GGCC 3 cut(s) 123, 989, 1191
Bsp1286I GDGCHC 1 cut(s) 340
Bsp143I GATC 3 cut(s) 112, 894, 976
BspACI CCGC 3 cut(s) 98, 766, 1235
BspANI GGCC 3 cut(s) 123, 989, 1191
BspCNI CTCAG 3 cut(s) 247, 1086, 1261
BspDI ATCGAT 1 cut(s) 979
BspPI GGATC 1 cut(s) 889
BspQI GCTCTTC 1 cut(s) 1323
BspTI CTTAAG 1 cut(s) 1121
BsrBI CCGCTC 1 cut(s) 766
BsrI ACTGG 2 cut(s) 727, 1319
BssECI CCNNGG 3 cut(s) 461, 462, 990
BssMI GATC 3 cut(s) 112, 894, 976
BssT1I CCWWGG 1 cut(s) 990
Bst2UI CCWGG 4 cut(s) 312, 463, 882, 1193
Bst4CI ACNGT 3 cut(s) 544, 830, 1023
Bst6I CTCTTC 3 cut(s) 944, 1195, 1323
BstAFI CTTAAG 1 cut(s) 1121
BstC8I GCNNGC 4 cut(s) 143, 290, 509, 728
BstDEI CTNAG 5 cut(s) 255, 329, 958, 1094, 1269
BstF5I GGATG 2 cut(s) 143, 1192
BstKTI GATC 3 cut(s) 115, 897, 979
BstMBI GATC 3 cut(s) 112, 894, 976
BstMCI CGRYCG 1 cut(s) 1239
BstMWI GCNNNNNNNGC 4 cut(s) 53, 86, 635, 1014
BstNI CCWGG 4 cut(s) 312, 463, 882, 1193
BstNSI RCATGY 3 cut(s) 496, 730, 1157
BstSCI CCNGG 4 cut(s) 310, 461, 880, 1191
BstSFI CTRYAG 2 cut(s) 81, 618
BstSLI GKGCMC 1 cut(s) 340
BstV1I GCAGC 2 cut(s) 436, 625
BstX2I RGATCY 1 cut(s) 112
BstYI RGATCY 1 cut(s) 112
Bsu15I ATCGAT 1 cut(s) 979
BsuRI GGCC 3 cut(s) 123, 989, 1191
BsuTUI ATCGAT 1 cut(s) 979
BtrI CACGTC 1 cut(s) 518
BtsCI GGATG 2 cut(s) 143, 1192
BtsIMutI CAGTG 2 cut(s) 705, 720
Cac8I GCNNGC 4 cut(s) 143, 290, 509, 728
CaiI CAGNNNCTG 1 cut(s) 1093
Cfr13I GGNCC 1 cut(s) 987
ClaI ATCGAT 1 cut(s) 979
Csp6I GTAC 2 cut(s) 545, 796
CviQI GTAC 2 cut(s) 545, 796
DdeI CTNAG 5 cut(s) 255, 329, 958, 1094, 1269
DpnI GATC 3 cut(s) 114, 896, 978
DpnII GATC 3 cut(s) 112, 894, 976
DraI TTTAAA 1 cut(s) 198
Eam1104I CTCTTC 3 cut(s) 944, 1195, 1323
EarI CTCTTC 3 cut(s) 944, 1195, 1323
Eco130I CCWWGG 1 cut(s) 990
Eco57I CTGAAG 4 cut(s) 547, 591, 968, 1098
EcoO109I RGGNCCY 1 cut(s) 987
EcoRII CCWGG 4 cut(s) 310, 461, 880, 1191
EcoT14I CCWWGG 1 cut(s) 990
ErhI CCWWGG 1 cut(s) 990
FalI AAGNNNNNCTT 4 cut(s) 99, 131, 941, 973
FaqI GGGAC 2 cut(s) 195, 863
Fnu4HI GCNGC 2 cut(s) 425, 614
FokI GGATG 2 cut(s) 150, 1199
Fsp4HI GCNGC 2 cut(s) 425, 614
GluI GCNGC 2 cut(s) 425, 614
GsuI CTGGAG 2 cut(s) 333, 855
HaeIII GGCC 3 cut(s) 123, 989, 1191
HindIII AAGCTT 1 cut(s) 866
HinfI GANTC 2 cut(s) 715, 1072
HphI GGTGA 2 cut(s) 683, 896
Hpy166II GTNNAC 2 cut(s) 206, 338
Hpy188I TCNGA 3 cut(s) 571, 810, 1110
Hpy8I GTNNAC 2 cut(s) 206, 338
HpyAV CCTTC 2 cut(s) 277, 887
HpyCH4III ACNGT 3 cut(s) 544, 830, 1023
HpyCH4IV ACGT 3 cut(s) 37, 208, 517
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 86, 635, 1014
HpyF3I CTNAG 5 cut(s) 255, 329, 958, 1094, 1269
HpySE526I ACGT 3 cut(s) 37, 208, 517
Kzo9I GATC 3 cut(s) 112, 894, 976
LguI GCTCTTC 1 cut(s) 1323
LmnI GCTCC 2 cut(s) 435, 997
Lsp1109I GCAGC 2 cut(s) 436, 625
LweI GCATC 5 cut(s) 67, 128, 364, 447, 1080
MaeII ACGT 3 cut(s) 37, 208, 517
MaeIII GTNAC 3 cut(s) 689, 838, 1258
MalI GATC 3 cut(s) 114, 896, 978
MbiI CCGCTC 1 cut(s) 766
MboI GATC 3 cut(s) 112, 894, 976
MboII GAAGA 6 cut(s) 86, 122, 961, 1175, 1212, 1340
MflI RGATCY 1 cut(s) 112
MhlI GDGCHC 1 cut(s) 340
MluCI AATT 9 cut(s) 162, 399, 414, 467, 487, 591, 640, 887, 1214
MlyI GAGTC 1 cut(s) 1066
MseI TTAA 7 cut(s) 197, 375, 552, 677, 1122, 1209, 1226
MslI CAYNNNNRTG 2 cut(s) 452, 725
MspA1I CMGCKG 2 cut(s) 100, 1268
MspCI CTTAAG 1 cut(s) 1121
MspR9I CCNGG 4 cut(s) 312, 463, 882, 1193
Mva1269I GAATGC 1 cut(s) 438
MvaI CCWGG 4 cut(s) 312, 463, 882, 1193
MwoI GCNNNNNNNGC 4 cut(s) 53, 86, 635, 1014
NdeII GATC 3 cut(s) 112, 894, 976
NmuCI GTSAC 2 cut(s) 689, 838
NspI RCATGY 3 cut(s) 496, 730, 1157
PaeI GCATGC 1 cut(s) 730
PasI CCCWGGG 1 cut(s) 462
PciSI GCTCTTC 1 cut(s) 1323
PctI GAATGC 1 cut(s) 438
PfeI GAWTC 1 cut(s) 715
PflMI CCANNNNNTGG 1 cut(s) 503
PkrI GCNGC 2 cut(s) 426, 615
PleI GAGTC 1 cut(s) 1066
PpsI GAGTC 1 cut(s) 1066
Psp6I CCWGG 4 cut(s) 310, 461, 880, 1191
PspGI CCWGG 4 cut(s) 310, 461, 880, 1191
PspPI GGNCC 1 cut(s) 987
PsrI GAACNNNNNNTAC 2 cut(s) 814, 846
PstNI CAGNNNCTG 1 cut(s) 1093
PsuI RGATCY 1 cut(s) 112
PvuII CAGCTG 1 cut(s) 1268
RsaI GTAC 2 cut(s) 546, 797
RsaNI GTAC 2 cut(s) 545, 796
RseI CAYNNNNRTG 2 cut(s) 452, 725
SapI GCTCTTC 1 cut(s) 1323
SaqAI TTAA 7 cut(s) 197, 375, 552, 677, 1122, 1209, 1226
SatI GCNGC 2 cut(s) 425, 614
Sau3AI GATC 3 cut(s) 112, 894, 976
Sau96I GGNCC 1 cut(s) 987
SchI GAGTC 1 cut(s) 1066
ScrFI CCNGG 4 cut(s) 312, 463, 882, 1193
SduI GDGCHC 1 cut(s) 340
SfaNI GCATC 5 cut(s) 67, 128, 364, 447, 1080
SfcI CTRYAG 2 cut(s) 81, 618
SmiMI CAYNNNNRTG 2 cut(s) 452, 725
SmlI CTYRAG 3 cut(s) 305, 1121, 1148
SmoI CTYRAG 3 cut(s) 305, 1121, 1148
SphI GCATGC 1 cut(s) 730
Sse9I AATT 9 cut(s) 162, 399, 414, 467, 487, 591, 640, 887, 1214
SsiI CCGC 3 cut(s) 98, 766, 1235
StyD4I CCNGG 4 cut(s) 310, 461, 880, 1191
StyI CCWWGG 1 cut(s) 990
TaaI ACNGT 3 cut(s) 544, 830, 1023
TaiI ACGT 3 cut(s) 40, 211, 520
TaqI TCGA 4 cut(s) 192, 825, 979, 1239
TasI AATT 9 cut(s) 162, 399, 414, 467, 487, 591, 640, 887, 1214
TatI WGTACW 2 cut(s) 544, 795
TfiI GAWTC 1 cut(s) 715
Tru1I TTAA 7 cut(s) 197, 375, 552, 677, 1122, 1209, 1226
Tru9I TTAA 7 cut(s) 197, 375, 552, 677, 1122, 1209, 1226
TscAI CASTG 2 cut(s) 712, 727
TseFI GTSAC 2 cut(s) 689, 838
TseI GCWGC 2 cut(s) 424, 613
Tsp45I GTSAC 2 cut(s) 689, 838
TspDTI ATGAA 3 cut(s) 596, 860, 1245
TspRI CASTG 2 cut(s) 712, 727
Van91I CCANNNNNTGG 1 cut(s) 503
Vha464I CTTAAG 1 cut(s) 1121
VneI GTGCAC 1 cut(s) 336
XapI RAATTY 2 cut(s) 591, 640
XceI RCATGY 3 cut(s) 496, 730, 1157
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.