Rorug07G0043500

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001519
Physical Location & Seq
Forward (+)
3184459 .. 3190335
5877 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0043500.1

Sequence Viewer

Length: 2217 bp
ATGACACGTGTCCGCATCCCATTGGAAACAGCCCATCGATCAGGCCGTGCTTGCTTCCAAGTCGTCGTTTTCACCTTTGGGCTCAACGCCGAGAACTCCTCACTCTCTCTCTCGCTCGCCGGCAAGATGGCAATCCCTGCTGCTTCTGCGGCGGCGAAGGCGACGAGCTCGTCGTCAATGGCGTCGACGATCAGAGCCTACTCTCTGCCTCTGATTCTCTTCATCGTCGCCATGTATTTCCAGCTCTCTGTTATTCCCAAATCCTTCCCTCCTTCTCACTACGCCGTTCTTGGTCTGAAGAGTCAGAGCTCCATTGAAGAAGTGAAAGAGGCCTACGACAACGTTTCTTCGACGTGGAATTCAGAAGCGCAAGCTCCGGATTCTCTCGAATTTATAAAGATTCGATATGCTTATGAGTTGCTCACAAATCCATCATGGAAAAGAAACTATGACACTTTTGGCTTTGAGGAGCAAATAGATGTAATTGAGAAGCTCAAAAAGCAATATGCTGGAGAAAGCTTTTCACGGATAGACCTTCCTTTACTAGAGTCTACTGCTTCTGATTCTGAAGATCATAACCTTCCAGTCATTACCTCTAAGGATTTCCAGACCATGTTTCAGGACAACAAGCCCTGGCTAATTCAGTTGTGCTCATTTGGGAGCAAAAGCTGTGATCAATTTTCTCATGCCTGGAAGAGAATTGCTGGTTTCTTGGATGGTGTGGCAAAAGCTGCTCAGGTAGATCTTGAAGATATTCAGCTAGCAACATATCTTGCTGACAAAAAACCAACCGGACAACCTTTCTTCAGGAATAGCCTTCCTTCTTTTGTTGCTTTTCCCCAGGGATGTAAAAGTGCCAAGTGCCTTATTAGGTATGAAGGAGACCCCTCAATTGATTCAGTTACAGATTGGTTTGCAACAACCATACTTGGTTTACCTCGTATTCTTTACTACTCAAAGGAGTCACTGGGGGAGAAGTTTCTAGCAAAAGTTAGTCTTCATAAGGTAAAAGTCATTTTCTTCTCAAAAACAGGGGAGCGTGCAGCTCCAATTGTACGCCAAGCTGCTAAAAGTTATTGGGCTCATGCTTCTTTTGCATTTGTGCTGTGGCGAGAAGAGGAATCTTCCTTTTGGTTCAATTCGTTTGAAGTTGAATCTGCACCTGCTATAGTGTTTCTGAAAGATCCTGGTGTCAAACCTGTTGTGTACCATGGGTCTTTCAGCAATTCACGTCTCTTAGATATCATCGAACAGAATAAAGAGCAAGAGCTTCCTCAGTTGAGGAGTACAACATCTATGCAATTGGGTTGCGATGCCCAAGGGTATTCTCGTGCTGGATACGATACCGTTACTTGGTACTGTGCTGTTGTAGCAGGAAGGCATAGCCCACAACTCAGTCAAATGCGCAGAACCTTGCGCAGGGTTCAAGAAACTTTATCGAATGAGGTTGAGTCGATACCGGCTGATGAAGAACAAACAGCACCAGCAGCACGTGCACTTCAAAGTAAACGACTGACATTTGCTTGGCTTGATGGGGAAGCCCAAAAGAAATATTGTCTCTTCTTCCTCCAATCTGAAACCAGCTATGAAACTTGTGGATCAAGGACAGATATGACCGATGTACCTAAGTTATTTATTATACGCTACAAAAGGAATGTATCAGAAGAGAGTGAGAAGCCAGAGGGAAAGCCAAAAAGCATATGGGATTCACTTCAGGACCAGGAATTAGATCCTGCAGCACAGCTTGTGGCAAAGTACAATGGTTCAGATGAAATTCCAGAGATTACCAAATGGATCTCCCAAATAATCAAGGATGGTGACGCCAGAGACCTTCCTCACTATAGAACAAGAACTCCTCAACTGGTTCCTGATGATTCGGAACCAATATGGTCCAGCAGTATTCAACGTATTCCTTCAACAAAGACGATAAAGCAGAGCATCATTGGCATTTTACGTGGAGTATATGATCGTATGGAAGATCCAAGAATTGGTCCGATCTTGCTTCTGGCAGCACTAATGTCTTTCGGTAACATCTGGCTTCGAAGGAGTCAAGCAACTCCATTGTCAAATCAACCAAATTCCAAGAATAGAAGTAGTCGAAGGAGGAGAGACCGCAGTGGAGCAGCATCTACTCAAGACCTAGCTCCTTCTATTACTGATATGGAACCCAGAGATTCTTATGAAATGCCACTATCAGGTTCCGACTCCGATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

738

Amino Acids

82.94

Weight (kDa)

6.79

Isoelectric Point (pI)

58.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 93 - 151 1.6e-07 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 395
AarI CACCTGC 1 cut(s) 1171
AasI GACNNNNNNGTC 1 cut(s) 169
Acc16I TGCGCA 2 cut(s) 1406, 1418
Acc36I ACCTGC 1 cut(s) 1171
AccB7I CCANNNNNTGG 1 cut(s) 1988
AccI GTMKAC 2 cut(s) 185, 551
AccIII TCCGGA 1 cut(s) 376
AciI CCGC 4 cut(s) 13, 149, 152, 2113
AclI AACGTT 1 cut(s) 342
AclWI GGATC 5 cut(s) 1178, 1606, 1724, 1802, 1973
AcsI RAATTY 4 cut(s) 358, 389, 1773, 2077
AcuI CTGAAG 4 cut(s) 317, 588, 790, 1697
AcvI CACGTG 2 cut(s) 8, 1493
AcyI GRCGYC 2 cut(s) 182, 1821
AfaI GTAC 6 cut(s) 1056, 1208, 1288, 1358, 1623, 1757
AfiI CCNNNNNNNGG 4 cut(s) 1353, 1419, 1988, 2195
AflIII ACRYGT 2 cut(s) 5, 7
AgsI TTSAA 9 cut(s) 317, 749, 1138, 1148, 1154, 1427, 1502, 1904, 1917
AjiI CACGTC 2 cut(s) 354, 1232
AjnI CCWGG 5 cut(s) 632, 689, 840, 1186, 1719
AloI GAACNNNNNNTCC 2 cut(s) 1837, 1869
Alw21I GWGCWC 4 cut(s) 170, 311, 653, 1498
Alw26I GTCTC 5 cut(s) 876, 1238, 1562, 1821, 2103
Alw44I GTGCAC 1 cut(s) 1494
AlwI GGATC 5 cut(s) 1178, 1606, 1724, 1802, 1973
Aor13HI TCCGGA 1 cut(s) 376
AoxI GGCC 2 cut(s) 43, 330
ApaLI GTGCAC 1 cut(s) 1494
ApeKI GCWGC 8 cut(s) 140, 731, 1043, 1064, 1487, 1736, 2009, 2123
ApoI RAATTY 4 cut(s) 358, 389, 1773, 2077
Asp700I GAANNNNTTC 1 cut(s) 753
AspLEI GCGC 3 cut(s) 370, 1407, 1419
AspS9I GGNCC 3 cut(s) 1717, 1890, 1991
AsuHPI GGTGA 2 cut(s) 64, 1829
AsuII TTCGAA 1 cut(s) 2041
AsuNHI GCTAGC 1 cut(s) 760
AvaII GGWCC 3 cut(s) 1717, 1890, 1991
BaeGI GKGCMC 1 cut(s) 1498
BaeI ACNNNNGTAYC 2 cut(s) 1605, 1638
BanII GRGCYC 4 cut(s) 84, 170, 311, 1084
BauI CACGAG 1 cut(s) 1329
BbrPI CACGTG 2 cut(s) 8, 1493
BbsI GAAGAC 1 cut(s) 989
Bbv12I GWGCWC 4 cut(s) 170, 311, 653, 1498
BbvI GCAGC 8 cut(s) 127, 718, 1051, 1055, 1499, 1748, 2021, 2135
BccI CCATC 6 cut(s) 42, 121, 439, 710, 1526, 1808
BceAI ACGGC 2 cut(s) 30, 269
BciT130I CCWGG 5 cut(s) 634, 691, 842, 1188, 1721
BciVI GTATCC 1 cut(s) 1331
BclI TGATCA 1 cut(s) 673
BcoDI GTCTC 5 cut(s) 876, 1238, 1562, 1821, 2103
BfaI CTAG 4 cut(s) 545, 761, 983, 2141
BfmI CTRYAG 3 cut(s) 1167, 1734, 1840
BfuAI ACCTGC 1 cut(s) 1171
BfuI GTATCC 1 cut(s) 1331
BglII AGATCT 1 cut(s) 742
Bme1390I CCNGG 5 cut(s) 634, 691, 842, 1188, 1721
Bme18I GGWCC 3 cut(s) 1717, 1890, 1991
BmgBI CACGTC 2 cut(s) 354, 1232
BmgT120I GGNCC 3 cut(s) 1717, 1890, 1991
BmiI GGNNCC 4 cut(s) 1866, 1881, 2166, 2200
BmrFI CCNGG 5 cut(s) 634, 691, 842, 1188, 1721
BmrI ACTGGG 1 cut(s) 977
BmsI GCATC 4 cut(s) 24, 1303, 1947, 2135
BmtI GCTAGC 1 cut(s) 764
BmuI ACTGGG 1 cut(s) 977
BoxI GACNNNNGTC 1 cut(s) 8
BpiI GAAGAC 1 cut(s) 989
BplI GAGNNNNNCTC 2 cut(s) 83, 115
BpmI CTGGAG 1 cut(s) 531
Bpu10I CCTNAGC 1 cut(s) 735
Bpu14I TTCGAA 1 cut(s) 2041
BpuEI CTTGAG 1 cut(s) 2118
Bsa29I ATCGAT 1 cut(s) 37
BsaAI YACGTR 3 cut(s) 8, 1493, 1955
BsaBI GATNNNNATC 1 cut(s) 131
BsaHI GRCGYC 2 cut(s) 182, 1821
BsaI GGTCTC 3 cut(s) 876, 1821, 2103
BsaJI CCNNGG 5 cut(s) 632, 840, 841, 1210, 1318
BsaWI WCCGGW 2 cut(s) 376, 791
BsaXI ACNNNNNCTCC 2 cut(s) 1837, 1867
Bsc4I CCNNNNNNNGG 4 cut(s) 1353, 1419, 1988, 2195
Bse118I RCCGGY 2 cut(s) 119, 1459
Bse1I ACTGG 3 cut(s) 584, 972, 1866
Bse8I GATNNNNATC 1 cut(s) 131
BseAI TCCGGA 1 cut(s) 376
BseBI CCWGG 5 cut(s) 634, 691, 842, 1188, 1721
BseCI ATCGAT 1 cut(s) 37
BseDI CCNNGG 5 cut(s) 632, 840, 841, 1210, 1318
BseGI GGATG 4 cut(s) 15, 721, 851, 1819
BseJI GATNNNNATC 1 cut(s) 131
BseLI CCNNNNNNNGG 4 cut(s) 1353, 1419, 1988, 2195
BseMII CTCAG 3 cut(s) 749, 1289, 1408
BseNI ACTGG 3 cut(s) 584, 972, 1866
BseRI GAGGAG 5 cut(s) 88, 482, 1297, 1845, 2119
BseSI GKGCMC 1 cut(s) 1498
BseXI GCAGC 8 cut(s) 127, 718, 1051, 1055, 1499, 1748, 2021, 2135
BsgI GTGCAG 2 cut(s) 1062, 1143
BshFI GGCC 2 cut(s) 45, 332
BshVI ATCGAT 1 cut(s) 37
BsiHKAI GWGCWC 4 cut(s) 170, 311, 653, 1498
BsiSI CCGG 4 cut(s) 120, 377, 792, 1460
BslI CCNNNNNNNGG 4 cut(s) 1353, 1419, 1988, 2195
BsmAI GTCTC 5 cut(s) 876, 1238, 1562, 1821, 2103
BsmBI CGTCTC 1 cut(s) 1238
BsnI GGCC 2 cut(s) 45, 332
Bso31I GGTCTC 3 cut(s) 876, 1821, 2103
Bsp119I TTCGAA 1 cut(s) 2041
Bsp1286I GDGCHC 6 cut(s) 84, 170, 311, 653, 1084, 1498
Bsp13I TCCGGA 1 cut(s) 376
Bsp19I CCATGG 1 cut(s) 1210
BspACI CCGC 4 cut(s) 13, 149, 152, 2113
BspANI GGCC 2 cut(s) 45, 332
BspCNI CTCAG 3 cut(s) 748, 1288, 1407
BspDI ATCGAT 1 cut(s) 37
BspEI TCCGGA 1 cut(s) 376
BspLI GGNNCC 4 cut(s) 1866, 1881, 2166, 2200
BspMAI CTGCAG 1 cut(s) 1738
BspMI ACCTGC 1 cut(s) 1171
BspOI GCTAGC 1 cut(s) 764
BspPI GGATC 5 cut(s) 1178, 1606, 1724, 1802, 1973
BspT104I TTCGAA 1 cut(s) 2041
BspTNI GGTCTC 3 cut(s) 876, 1821, 2103
BsrFI RCCGGY 2 cut(s) 119, 1459
BsrI ACTGG 3 cut(s) 584, 972, 1866
BssAI RCCGGY 2 cut(s) 119, 1459
BssECI CCNNGG 5 cut(s) 632, 840, 841, 1210, 1318
BssNI GRCGYC 2 cut(s) 182, 1821
BssSI CACGAG 1 cut(s) 1329
BssT1I CCWWGG 2 cut(s) 1210, 1318
Bst2BI CACGAG 1 cut(s) 1329
Bst2UI CCWGG 5 cut(s) 634, 691, 842, 1188, 1721
Bst4CI ACNGT 2 cut(s) 1348, 1361
Bst6I CTCTTC 6 cut(s) 224, 293, 689, 1110, 1565, 1659
BstACI GRCGYC 2 cut(s) 182, 1821
BstAPI GCANNNNNTGC 3 cut(s) 137, 731, 1493
BstBAI YACGTR 3 cut(s) 8, 1493, 1955
BstBI TTCGAA 1 cut(s) 2041
BstC8I GCNNGC 6 cut(s) 52, 117, 121, 372, 762, 1041
BstDEI CTNAG 6 cut(s) 597, 735, 1237, 1275, 1394, 1626
BstDSI CCRYGG 1 cut(s) 1210
BstENI CCTNNNNNAGG 1 cut(s) 1417
BstF5I GGATG 4 cut(s) 15, 721, 851, 1819
BstHHI GCGC 3 cut(s) 370, 1407, 1419
BstMAI GTCTC 5 cut(s) 876, 1238, 1562, 1821, 2103
BstNI CCWGG 5 cut(s) 634, 691, 842, 1188, 1721
BstPAI GACNNNNGTC 1 cut(s) 8
BstSCI CCNGG 5 cut(s) 632, 689, 840, 1186, 1719
BstSFI CTRYAG 3 cut(s) 1167, 1734, 1840
BstSLI GKGCMC 1 cut(s) 1498
BstV1I GCAGC 8 cut(s) 127, 718, 1051, 1055, 1499, 1748, 2021, 2135
BstV2I GAAGAC 1 cut(s) 989
BstX2I RGATCY 5 cut(s) 742, 1183, 1729, 1794, 1978
BstYI RGATCY 5 cut(s) 742, 1183, 1729, 1794, 1978
Bsu15I ATCGAT 1 cut(s) 37
BsuI GTATCC 1 cut(s) 1331
BsuRI GGCC 2 cut(s) 45, 332
BsuTUI ATCGAT 1 cut(s) 37
BtgI CCRYGG 1 cut(s) 1210
BtgZI GCGATG 1 cut(s) 1326
BtrI CACGTC 2 cut(s) 354, 1232
BtsCI GGATG 4 cut(s) 15, 721, 851, 1819
BtsI GCAGTG 1 cut(s) 2122
BtsIMutI CAGTG 2 cut(s) 965, 2122
BveI ACCTGC 1 cut(s) 1171
Cac8I GCNNGC 6 cut(s) 52, 117, 121, 372, 762, 1041
CfoI GCGC 3 cut(s) 370, 1407, 1419
Cfr10I RCCGGY 2 cut(s) 119, 1459
Cfr13I GGNCC 3 cut(s) 1717, 1890, 1991
ClaI ATCGAT 1 cut(s) 37
CseI GACGC 2 cut(s) 171, 1829
Csp6I GTAC 6 cut(s) 1055, 1207, 1287, 1357, 1622, 1756
CviAII CATG 6 cut(s) 232, 435, 613, 686, 1085, 1211
CviQI GTAC 6 cut(s) 1055, 1207, 1287, 1357, 1622, 1756
DdeI CTNAG 6 cut(s) 597, 735, 1237, 1275, 1394, 1626
DrdI GACNNNNNNGTC 1 cut(s) 169
DseDI GACNNNNNNGTC 1 cut(s) 169
Eam1104I CTCTTC 6 cut(s) 224, 293, 689, 1110, 1565, 1659
EarI CTCTTC 6 cut(s) 224, 293, 689, 1110, 1565, 1659
Ecl136II GAGCTC 2 cut(s) 168, 309
Eco130I CCWWGG 2 cut(s) 1210, 1318
Eco147I AGGCCT 1 cut(s) 332
Eco24I GRGCYC 4 cut(s) 84, 170, 311, 1084
Eco31I GGTCTC 3 cut(s) 876, 1821, 2103
Eco32I GATATC 1 cut(s) 1243
Eco47I GGWCC 3 cut(s) 1717, 1890, 1991
Eco53kI GAGCTC 2 cut(s) 168, 309
Eco57I CTGAAG 4 cut(s) 317, 588, 790, 1697
Eco72I CACGTG 2 cut(s) 8, 1493
EcoICRI GAGCTC 2 cut(s) 168, 309
EcoNI CCTNNNNNAGG 1 cut(s) 1417
EcoRI GAATTC 1 cut(s) 358
EcoRII CCWGG 5 cut(s) 632, 689, 840, 1186, 1719
EcoRV GATATC 1 cut(s) 1243
EcoT14I CCWWGG 2 cut(s) 1210, 1318
EcoT38I GRGCYC 4 cut(s) 84, 170, 311, 1084
ErhI CCWWGG 2 cut(s) 1210, 1318
Esp3I CGTCTC 1 cut(s) 1238
FaeI CATG 6 cut(s) 235, 438, 616, 689, 1088, 1214
FalI AAGNNNNNCTT 2 cut(s) 981, 1013
FatI CATG 6 cut(s) 231, 434, 612, 685, 1084, 1210
FauNDI CATATG 1 cut(s) 1700
FbaI TGATCA 1 cut(s) 673
FblI GTMKAC 2 cut(s) 185, 551
FokI GGATG 4 cut(s) 2, 728, 858, 1826
FriOI GRGCYC 4 cut(s) 84, 170, 311, 1084
FspBI CTAG 4 cut(s) 545, 761, 983, 2141
FspI TGCGCA 2 cut(s) 1406, 1418
GlaI GCGC 3 cut(s) 369, 1406, 1418
GsuI CTGGAG 1 cut(s) 531
HaeIII GGCC 2 cut(s) 45, 332
HapII CCGG 4 cut(s) 120, 377, 792, 1460
HgaI GACGC 2 cut(s) 171, 1829
HhaI GCGC 3 cut(s) 370, 1407, 1419
Hin1I GRCGYC 2 cut(s) 182, 1821
Hin1II CATG 6 cut(s) 235, 438, 616, 689, 1088, 1214
Hin6I GCGC 3 cut(s) 368, 1405, 1417
HinP1I GCGC 3 cut(s) 368, 1405, 1417
HincII GTYRAC 1 cut(s) 186
HindII GTYRAC 1 cut(s) 186
HindIII AAGCTT 1 cut(s) 517
HpaII CCGG 4 cut(s) 120, 377, 792, 1460
HphI GGTGA 2 cut(s) 64, 1829
Hpy166II GTNNAC 6 cut(s) 186, 552, 935, 1207, 1496, 1508
Hpy8I GTNNAC 6 cut(s) 186, 552, 935, 1207, 1496, 1508
Hpy99I CGWCG 7 cut(s) 68, 166, 175, 187, 190, 230, 355
HpyCH4III ACNGT 2 cut(s) 1348, 1361
HpyCH4IV ACGT 7 cut(s) 7, 342, 353, 1231, 1492, 1906, 1954
HpyCH4V TGCA 7 cut(s) 917, 1043, 1097, 1160, 1300, 1496, 1736
HpyF3I CTNAG 6 cut(s) 597, 735, 1237, 1275, 1394, 1626
HpySE526I ACGT 7 cut(s) 7, 342, 353, 1231, 1492, 1906, 1954
Hsp92I GRCGYC 2 cut(s) 182, 1821
Hsp92II CATG 6 cut(s) 235, 438, 616, 689, 1088, 1214
HspAI GCGC 3 cut(s) 368, 1405, 1417
Kpn2I TCCGGA 1 cut(s) 376
KroI GCCGGC 1 cut(s) 119
KroNI GCCGGC 1 cut(s) 121
Ksp22I TGATCA 1 cut(s) 673
LmnI GCTCC 8 cut(s) 314, 379, 469, 660, 1036, 1051, 2120, 2149
Lsp1109I GCAGC 8 cut(s) 127, 718, 1051, 1055, 1499, 1748, 2021, 2135
LweI GCATC 4 cut(s) 24, 1303, 1947, 2135
MaeI CTAG 4 cut(s) 545, 761, 983, 2141
MaeII ACGT 7 cut(s) 7, 342, 353, 1231, 1492, 1906, 1954
MaeIII GTNAC 5 cut(s) 901, 963, 1348, 1817, 2027
MfeI CAATTG 3 cut(s) 891, 1050, 1301
MflI RGATCY 5 cut(s) 742, 1183, 1729, 1794, 1978
MhlI GDGCHC 6 cut(s) 84, 170, 311, 653, 1084, 1498
MlyI GAGTC 6 cut(s) 310, 557, 971, 1460, 2056, 2198
MroI TCCGGA 1 cut(s) 376
MroNI GCCGGC 1 cut(s) 119
MroXI GAANNNNTTC 1 cut(s) 753
MspI CCGG 4 cut(s) 120, 377, 792, 1460
MspR9I CCNGG 5 cut(s) 634, 691, 842, 1188, 1721
MunI CAATTG 3 cut(s) 891, 1050, 1301
MvaI CCWGG 5 cut(s) 634, 691, 842, 1188, 1721
NaeI GCCGGC 1 cut(s) 121
NcoI CCATGG 1 cut(s) 1210
NdeI CATATG 1 cut(s) 1700
NgoMIV GCCGGC 1 cut(s) 119
NheI GCTAGC 1 cut(s) 760
NlaIII CATG 6 cut(s) 235, 438, 616, 689, 1088, 1214
NlaIV GGNNCC 4 cut(s) 1866, 1881, 2166, 2200
NmeAIII GCCGAG 1 cut(s) 115
NmuCI GTSAC 2 cut(s) 963, 1817
NsbI TGCGCA 2 cut(s) 1406, 1418
NspV TTCGAA 1 cut(s) 2041
PaqCI CACCTGC 1 cut(s) 1171
PasI CCCWGGG 1 cut(s) 841
PceI AGGCCT 1 cut(s) 332
PcsI WCGNNNNNNNCGW 3 cut(s) 43, 170, 179
PdiI GCCGGC 1 cut(s) 121
PdmI GAANNNNTTC 1 cut(s) 753
PflMI CCANNNNNTGG 1 cut(s) 1988
PleI GAGTC 6 cut(s) 309, 556, 970, 1459, 2055, 2198
PmaCI CACGTG 2 cut(s) 8, 1493
PmlI CACGTG 2 cut(s) 8, 1493
PpsI GAGTC 6 cut(s) 309, 556, 970, 1459, 2055, 2198
Ppu21I YACGTR 3 cut(s) 8, 1493, 1955
PshAI GACNNNNGTC 1 cut(s) 8
PsiI TTATAA 1 cut(s) 395
Psp124BI GAGCTC 2 cut(s) 170, 311
Psp1406I AACGTT 1 cut(s) 342
Psp6I CCWGG 5 cut(s) 632, 689, 840, 1186, 1719
PspCI CACGTG 2 cut(s) 8, 1493
PspGI CCWGG 5 cut(s) 632, 689, 840, 1186, 1719
PspN4I GGNNCC 4 cut(s) 1866, 1881, 2166, 2200
PspPI GGNCC 3 cut(s) 1717, 1890, 1991
PstI CTGCAG 1 cut(s) 1738
PsuI RGATCY 5 cut(s) 742, 1183, 1729, 1794, 1978
RsaI GTAC 6 cut(s) 1056, 1208, 1288, 1358, 1623, 1757
RsaNI GTAC 6 cut(s) 1055, 1207, 1287, 1357, 1622, 1756
SacI GAGCTC 2 cut(s) 170, 311
SalI GTCGAC 1 cut(s) 184
Sau96I GGNCC 3 cut(s) 1717, 1890, 1991
SchI GAGTC 6 cut(s) 310, 557, 971, 1460, 2056, 2198
ScrFI CCNGG 5 cut(s) 634, 691, 842, 1188, 1721
SduI GDGCHC 6 cut(s) 84, 170, 311, 653, 1084, 1498
SfaNI GCATC 4 cut(s) 24, 1303, 1947, 2135
SfcI CTRYAG 3 cut(s) 1167, 1734, 1840
SfuI TTCGAA 1 cut(s) 2041
SgrDI CGTCGACG 1 cut(s) 184
SinI GGWCC 3 cut(s) 1717, 1890, 1991
SmlI CTYRAG 1 cut(s) 2133
SmoI CTYRAG 1 cut(s) 2133
SseBI AGGCCT 1 cut(s) 332
SsiI CCGC 4 cut(s) 13, 149, 152, 2113
SspI AATATT 1 cut(s) 1553
SspMI CTAG 4 cut(s) 545, 761, 983, 2141
SstI GAGCTC 2 cut(s) 170, 311
StuI AGGCCT 1 cut(s) 332
StyD4I CCNGG 5 cut(s) 632, 689, 840, 1186, 1719
StyI CCWWGG 2 cut(s) 1210, 1318
TaaI ACNGT 2 cut(s) 1348, 1361
TaiI ACGT 7 cut(s) 10, 345, 356, 1234, 1495, 1909, 1957
TaqII GACCGA 1 cut(s) 1631
TatI WGTACW 2 cut(s) 1286, 1755
TauI GCSGC 2 cut(s) 152, 155
TscAI CASTG 2 cut(s) 972, 2122
TseFI GTSAC 2 cut(s) 963, 1817
TseI GCWGC 8 cut(s) 140, 731, 1043, 1064, 1487, 1736, 2009, 2123
Tsp45I GTSAC 2 cut(s) 963, 1817
TspDTI ATGAA 7 cut(s) 211, 891, 989, 1482, 1602, 1785, 2196
TspGWI ACGGA 1 cut(s) 541
TspRI CASTG 2 cut(s) 972, 2122
Van91I CCANNNNNTGG 1 cut(s) 1988
VneI GTGCAC 1 cut(s) 1494
VpaK11BI GGWCC 3 cut(s) 1717, 1890, 1991
XagI CCTNNNNNAGG 1 cut(s) 1417
XapI RAATTY 4 cut(s) 358, 389, 1773, 2077
XcmI CCANNNNNNNNNTGG 1 cut(s) 1698
XmiI GTMKAC 2 cut(s) 185, 551
XmnI GAANNNNTTC 1 cut(s) 753
XspI CTAG 4 cut(s) 545, 761, 983, 2141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.