Rh7CG176300

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0001519
Physical Location & Seq
Forward (+)
15055021 .. 15058176
3156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG176300.1

Sequence Viewer

Length: 1356 bp
ATGGCACTCATTTCCCAGACAAGTTCATCATACTACTGCAGAAGCATTGGCTTCTCTTCCTCTGCATTTTATTCTACTACTACTACTACTAGGAAGATACAGGTTTTGCATGTAGAAAATGTTGCAGGACAGAAACGAGGACGTAAGAGTGTGATAGTTGCTGCAAGTCCTATCACAGAAGATGCTGTAGTAGCTACAAAGCCGCTGATGAAAGAAGATCTTGTAGGCTATCTTGCCTCTGGATGCAAGCCCAAAGAAAAATGGAGAATTGGCATGGAACACGAGAGGTTTGGCTTCGAACTAAATACTTTACGTCCGATGCAATATGAACAAATAGCTGAGCTGCTCTATGGGATTTCTGGGAGATTTGAGTGGGAGAAAATCATGGAAGGTGGTAAGATTATTGGACTCAAACAGGGTAAGCAGAGCATATCACTAGAGCCAGGAGGTCAATTTGAGCTTAGTGGTGCACCTCTTGAAACTCTGCATCAAATTTGTATTGAAGTCAATTCACAACTCTGTCAGGTCAAAGCAGTTGCAGAGAAAATGGGAATCGGATTTCTTGAAATTGGTGGTCAGCCGAAATGGAGAATTCAAGACATACCTATGATGCCCAAGGGAAGATATGAGATTATTAGAAATTACATGCCAAAAGTTGGCTCGCTTGGACATGATTTGATGTTCAGAACTTGCACTGTACAGGTCAATCTGGACTTCAGTTCTGAAGCAGACATGAAACGGAAATTGCGTGCTAGTCTTGCTTTGCAGCCTATAGCAACAGCCCTATTTGCAAATTCACCTTTCACTGAAGGAAAACCGAATGGTTTTCTAAGCAGGAGAAGTCACATCTATACTGATACTGACAAGGCTCGCACTGGCATGCTGCCTTTTGCTTTTGATGACTCCTTTGGGTTTGAGCGGTATGTTGATTATGCTCTTGATGTTCCTATGTACTGTGTTTATCGGAAAAATAAGTGTATCGACTGTACTGGAACGACCTTTCGGGACTTTATGGCGGGAAAGCTTCGTTGCCTTCCAGGTGAATTACCAACACTGAATGATTGGGAGAATCATTTGGGAACTATTTATCCAGAGGTCAGGCTGAAGAGATTCTTGGAGATGAGGGGTGCTGATGGAGGGCCTTGGAGAAGCTTATGCTCTTTGCCTGCATTTTGGGTGGGTCTACTATATGATGAAGTTTCCCTGCAGAGTGTGTTAGACATGATTGCTGATTGGACTCCTGAAGAAAGACAGATGCTGAGAGATAAGATTCCAAAAACGGGTCTAAAGACACCATTTCGTGAAGGAATACTCAAGCATGTTGCCGAAGATGTAGTAAAGTTTGCAAAGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

451

Amino Acids

50.92

Weight (kDa)

8.75

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 89 - 447 7.4e-92 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 656
AccBSI CCGCTC 1 cut(s) 919
AccI GTMKAC 1 cut(s) 1183
AciI CCGC 3 cut(s) 203, 919, 1016
AcsI RAATTY 3 cut(s) 492, 591, 793
AcuI CTGAAG 5 cut(s) 700, 744, 828, 1124, 1263
AfaI GTAC 3 cut(s) 699, 953, 988
AfiI CCNNNNNNNGG 2 cut(s) 656, 1280
AgsI TTSAA 4 cut(s) 479, 503, 566, 596
AjnI CCWGG 2 cut(s) 442, 1036
AjuI GAANNNNNNNTTGG 2 cut(s) 1097, 1129
AloI GAACNNNNNNTCC 2 cut(s) 1072, 1104
AluBI AGCT 6 cut(s) 194, 338, 343, 460, 1024, 1152
AluI AGCT 6 cut(s) 194, 338, 343, 460, 1024, 1152
Alw21I GWGCWC 1 cut(s) 472
Alw44I GTGCAC 1 cut(s) 468
AlwNI CAGNNNCTG 1 cut(s) 1258
AoxI GGCC 1 cut(s) 1139
ApaLI GTGCAC 1 cut(s) 468
ApeKI GCWGC 4 cut(s) 161, 343, 766, 883
ApoI RAATTY 3 cut(s) 492, 591, 793
Asp700I GAANNNNTTC 1 cut(s) 1109
AspS9I GGNCC 1 cut(s) 1139
AsuHPI GGTGA 2 cut(s) 789, 1052
AsuII TTCGAA 1 cut(s) 297
BaeGI GKGCMC 1 cut(s) 472
BauI CACGAG 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 472
BbvI GCAGC 4 cut(s) 148, 330, 778, 870
BccI CCATC 1 cut(s) 1127
BciT130I CCWGG 2 cut(s) 444, 1038
BfaI CTAG 3 cut(s) 90, 437, 753
BfmI CTRYAG 4 cut(s) 37, 186, 771, 1205
BglII AGATCT 1 cut(s) 217
BisI GCNGC 5 cut(s) 162, 203, 344, 767, 884
BlpI GCTNAGC 1 cut(s) 339
BlsI GCNGC 5 cut(s) 163, 204, 345, 768, 885
Bme1390I CCNGG 2 cut(s) 444, 1038
BmgT120I GGNCC 1 cut(s) 1139
BmrFI CCNGG 2 cut(s) 444, 1038
BmsI GCATC 6 cut(s) 172, 233, 309, 496, 600, 1245
Bpu1102I GCTNAGC 1 cut(s) 339
Bpu14I TTCGAA 1 cut(s) 297
BpuEI CTTGAG 1 cut(s) 1298
BsaJI CCNNGG 2 cut(s) 615, 1142
Bsc4I CCNNNNNNNGG 2 cut(s) 656, 1280
Bse1I ACTGG 2 cut(s) 880, 994
BseBI CCWGG 2 cut(s) 444, 1038
BseDI CCNNGG 2 cut(s) 615, 1142
BseGI GGATG 1 cut(s) 248
BseLI CCNNNNNNNGG 2 cut(s) 656, 1280
BseMII CTCAG 2 cut(s) 330, 1250
BseNI ACTGG 2 cut(s) 880, 994
BseSI GKGCMC 1 cut(s) 472
BseXI GCAGC 4 cut(s) 148, 330, 778, 870
BshFI GGCC 1 cut(s) 1141
BsiHKAI GWGCWC 1 cut(s) 472
BslFI GGGAC 1 cut(s) 1019
BslI CCNNNNNNNGG 2 cut(s) 656, 1280
BsmFI GGGAC 1 cut(s) 1019
BsnI GGCC 1 cut(s) 1141
Bsp119I TTCGAA 1 cut(s) 297
Bsp1286I GDGCHC 1 cut(s) 472
Bsp1407I TGTACA 1 cut(s) 697
Bsp143I GATC 1 cut(s) 217
Bsp1720I GCTNAGC 1 cut(s) 339
BspACI CCGC 3 cut(s) 203, 919, 1016
BspANI GGCC 1 cut(s) 1141
BspCNI CTCAG 2 cut(s) 331, 1251
BspMAI CTGCAG 2 cut(s) 41, 1209
BspT104I TTCGAA 1 cut(s) 297
BsrBI CCGCTC 1 cut(s) 919
BsrGI TGTACA 1 cut(s) 697
BsrI ACTGG 2 cut(s) 880, 994
BssECI CCNNGG 2 cut(s) 615, 1142
BssMI GATC 1 cut(s) 217
BssSI CACGAG 1 cut(s) 281
BssT1I CCWWGG 2 cut(s) 615, 1142
Bst2BI CACGAG 1 cut(s) 281
Bst2UI CCWGG 2 cut(s) 444, 1038
Bst4CI ACNGT 3 cut(s) 697, 956, 986
Bst6I CTCTTC 2 cut(s) 61, 1100
BstAUI TGTACA 1 cut(s) 697
BstBI TTCGAA 1 cut(s) 297
BstC8I GCNNGC 6 cut(s) 248, 662, 750, 871, 881, 1167
BstDEI CTNAG 4 cut(s) 339, 461, 830, 1259
BstF5I GGATG 1 cut(s) 248
BstKTI GATC 1 cut(s) 220
BstMBI GATC 1 cut(s) 217
BstMWI GCNNNNNNNGC 3 cut(s) 191, 758, 788
BstNI CCWGG 2 cut(s) 444, 1038
BstNSI RCATGY 4 cut(s) 113, 649, 883, 1322
BstSCI CCNGG 2 cut(s) 442, 1036
BstSFI CTRYAG 4 cut(s) 37, 186, 771, 1205
BstSLI GKGCMC 1 cut(s) 472
BstV1I GCAGC 4 cut(s) 148, 330, 778, 870
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BsuRI GGCC 1 cut(s) 1141
BtsCI GGATG 1 cut(s) 248
BtsIMutI CAGTG 4 cut(s) 693, 804, 873, 1052
Cac8I GCNNGC 6 cut(s) 248, 662, 750, 871, 881, 1167
CaiI CAGNNNCTG 1 cut(s) 1258
Cfr13I GGNCC 1 cut(s) 1139
Csp6I GTAC 3 cut(s) 698, 952, 987
CviAII CATG 9 cut(s) 110, 274, 385, 646, 671, 733, 880, 1222, 1319
CviQI GTAC 3 cut(s) 698, 952, 987
DdeI CTNAG 4 cut(s) 339, 461, 830, 1259
DpnI GATC 1 cut(s) 219
DpnII GATC 1 cut(s) 217
Eam1104I CTCTTC 2 cut(s) 61, 1100
EarI CTCTTC 2 cut(s) 61, 1100
Eco130I CCWWGG 2 cut(s) 615, 1142
Eco57I CTGAAG 5 cut(s) 700, 744, 828, 1124, 1263
EcoO109I RGGNCCY 1 cut(s) 1139
EcoRI GAATTC 1 cut(s) 591
EcoRII CCWGG 2 cut(s) 442, 1036
EcoT14I CCWWGG 2 cut(s) 615, 1142
ErhI CCWWGG 2 cut(s) 615, 1142
FaeI CATG 9 cut(s) 113, 277, 388, 649, 674, 736, 883, 1225, 1322
FalI AAGNNNNNCTT 4 cut(s) 204, 236, 1097, 1129
FaqI GGGAC 1 cut(s) 1019
FatI CATG 9 cut(s) 109, 273, 384, 645, 670, 732, 879, 1221, 1318
FauI CCCGC 1 cut(s) 1009
FblI GTMKAC 1 cut(s) 1183
Fnu4HI GCNGC 5 cut(s) 162, 203, 344, 767, 884
FokI GGATG 1 cut(s) 255
Fsp4HI GCNGC 5 cut(s) 162, 203, 344, 767, 884
FspBI CTAG 3 cut(s) 90, 437, 753
GluI GCNGC 5 cut(s) 162, 203, 344, 767, 884
HaeIII GGCC 1 cut(s) 1141
Hin1II CATG 9 cut(s) 113, 277, 388, 649, 674, 736, 883, 1225, 1322
HindIII AAGCTT 2 cut(s) 1022, 1150
HinfI GANTC 7 cut(s) 408, 552, 902, 1069, 1110, 1237, 1270
HphI GGTGA 2 cut(s) 789, 1052
Hpy166II GTNNAC 2 cut(s) 470, 1184
Hpy188I TCNGA 5 cut(s) 318, 557, 686, 724, 966
Hpy8I GTNNAC 2 cut(s) 470, 1184
HpyAV CCTTC 4 cut(s) 383, 803, 1043, 1298
HpyCH4III ACNGT 3 cut(s) 697, 956, 986
HpyCH4IV ACGT 2 cut(s) 142, 313
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 758, 788
HpyF3I CTNAG 4 cut(s) 339, 461, 830, 1259
HpySE526I ACGT 2 cut(s) 142, 313
Hsp92II CATG 9 cut(s) 113, 277, 388, 649, 674, 736, 883, 1225, 1322
Kzo9I GATC 1 cut(s) 217
Lsp1109I GCAGC 4 cut(s) 148, 330, 778, 870
LweI GCATC 6 cut(s) 172, 233, 309, 496, 600, 1245
MaeI CTAG 3 cut(s) 90, 437, 753
MaeII ACGT 2 cut(s) 142, 313
MaeIII GTNAC 1 cut(s) 842
MalI GATC 1 cut(s) 219
MbiI CCGCTC 1 cut(s) 919
MboI GATC 1 cut(s) 217
MboII GAAGA 8 cut(s) 48, 106, 191, 227, 633, 1117, 1256, 1340
MflI RGATCY 1 cut(s) 217
MhlI GDGCHC 1 cut(s) 472
MlyI GAGTC 3 cut(s) 402, 896, 1231
MnlI CCTC 9 cut(s) 70, 131, 247, 279, 440, 483, 1087, 1116, 1130
MroXI GAANNNNTTC 1 cut(s) 1109
MslI CAYNNNNRTG 2 cut(s) 605, 878
MspA1I CMGCKG 1 cut(s) 205
MspR9I CCNGG 2 cut(s) 444, 1038
MvaI CCWGG 2 cut(s) 444, 1038
MwoI GCNNNNNNNGC 3 cut(s) 191, 758, 788
NdeII GATC 1 cut(s) 217
NlaIII CATG 9 cut(s) 113, 277, 388, 649, 674, 736, 883, 1225, 1322
NmuCI GTSAC 1 cut(s) 842
NspI RCATGY 4 cut(s) 113, 649, 883, 1322
NspV TTCGAA 1 cut(s) 297
PaeI GCATGC 1 cut(s) 883
PcsI WCGNNNNNNNCGW 1 cut(s) 745
PdmI GAANNNNTTC 1 cut(s) 1109
PfeI GAWTC 4 cut(s) 552, 1069, 1110, 1270
PflMI CCANNNNNTGG 1 cut(s) 656
PkrI GCNGC 5 cut(s) 163, 204, 345, 768, 885
PleI GAGTC 3 cut(s) 402, 896, 1231
PpsI GAGTC 3 cut(s) 402, 896, 1231
Psp6I CCWGG 2 cut(s) 442, 1036
PspGI CCWGG 2 cut(s) 442, 1036
PspPI GGNCC 1 cut(s) 1139
PstI CTGCAG 2 cut(s) 41, 1209
PstNI CAGNNNCTG 1 cut(s) 1258
PsuI RGATCY 1 cut(s) 217
RsaI GTAC 3 cut(s) 699, 953, 988
RsaNI GTAC 3 cut(s) 698, 952, 987
RseI CAYNNNNRTG 2 cut(s) 605, 878
SatI GCNGC 5 cut(s) 162, 203, 344, 767, 884
Sau3AI GATC 1 cut(s) 217
Sau96I GGNCC 1 cut(s) 1139
SchI GAGTC 3 cut(s) 402, 896, 1231
ScrFI CCNGG 2 cut(s) 444, 1038
SduI GDGCHC 1 cut(s) 472
SfaNI GCATC 6 cut(s) 172, 233, 309, 496, 600, 1245
SfcI CTRYAG 4 cut(s) 37, 186, 771, 1205
SfuI TTCGAA 1 cut(s) 297
SmiMI CAYNNNNRTG 2 cut(s) 605, 878
SmlI CTYRAG 1 cut(s) 1313
SmoI CTYRAG 1 cut(s) 1313
SphI GCATGC 1 cut(s) 883
SsiI CCGC 3 cut(s) 203, 919, 1016
SspMI CTAG 3 cut(s) 90, 437, 753
StyD4I CCNGG 2 cut(s) 442, 1036
StyI CCWWGG 2 cut(s) 615, 1142
TaaI ACNGT 3 cut(s) 697, 956, 986
TaiI ACGT 2 cut(s) 145, 316
TaqI TCGA 2 cut(s) 297, 981
TatI WGTACW 3 cut(s) 697, 951, 986
TauI GCSGC 1 cut(s) 205
TfiI GAWTC 4 cut(s) 552, 1069, 1110, 1270
TscAI CASTG 4 cut(s) 700, 811, 880, 1059
TseFI GTSAC 1 cut(s) 842
TseI GCWGC 4 cut(s) 161, 343, 766, 883
Tsp45I GTSAC 1 cut(s) 842
TspDTI ATGAA 5 cut(s) 15, 224, 342, 749, 1209
TspGWI ACGGA 1 cut(s) 754
TspRI CASTG 4 cut(s) 700, 811, 880, 1059
Van91I CCANNNNNTGG 1 cut(s) 656
VneI GTGCAC 1 cut(s) 468
XapI RAATTY 3 cut(s) 492, 591, 793
XceI RCATGY 4 cut(s) 113, 649, 883, 1322
XmiI GTMKAC 1 cut(s) 1183
XmnI GAANNNNTTC 1 cut(s) 1109
XspI CTAG 3 cut(s) 90, 437, 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.