Prupe.5G099600_v2.0.a1

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001519
Physical Location & Seq
Reverse (-)
10777193 .. 10781662
4470 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G099600.1

Sequence Viewer

Length: 1455 bp
ATGCATGTAGACAATGCTGAAGTAGGACAGAGGCGACGACGTAGTGCGATAGTTGCTGCAAGCCCTCCCACAGAAGCGGCTGTTATTGCTGCAGAGCCACTGACGAAAGAGGATCTTATCGGGTATCTTGCCTCTGGATGCAAGCCTAAAGAAAATTGGAGAATTGGCACTGAGCAGGAGAGATTTGGCTTTGATCTAAAGACTTTACGTCCTATGAAATATGAACACATTGCCGAGTTGCTTAATGGGATTGCTGATAGATTTGATTGGGATAAAATCATGGAAGGCAACAACATTATTGCACTCAAACAGGGTAAGCAGAGCATAACATTGGAGCCTGGAGGTCAATTCGAGCATAGTGGTGCACCTCTTGAAACTCTGCATCAAGTTTGTGCTGAAGTCAATTCTCATCTTTATCAGGTCAGTGAAAATAATTTTTCTTTTGAATTCACATGCAACTATGCAAGTGAGGTTAAAGCAGTTCTAAAGGAAATGGGAATCGGATTGCTTGATATTGGTTGTCAGCCTAAATGGGAAGTTAAAGATATACCTATGATGCCCAAGGAACGATACGAAATTATTAGAAATTACATGCCCAAAGTTGGCTCGCTCGGACTCGATTTGATGTTCAGGACTTGCACTGCACAGGTTAATCTGGACTTCAGTTCCGAAGCCGACATGATAAAGAAACTTCGTGCTAGTCTTGCTTTGCAGCCTATTGCAACAGCTCTATTTGCAAATTCACCTTTCACTGAAGGAAAGCCAAATGGTTTTCTTAGCATGAGAAGTCAAATTTGGACTGATACTGACAAGGATCGCACTGGCATGATTCCTTTTGTTTTCGATGACACCTTTGGGTTCGAGAAGTATGTTGAATATGCTCTGGATATTCCCATGTACTTTGTTTATCGGAACAAGAGGCATTTCGACTGTACTGGGATGACCTTCCGGGATTTCATGGCAGGAAAGCTTCGTTGCATTCCGGGTGAATTGCCAACCATGAACGATTGGGAGATGCATTTGGGAACTATATATCCTGAGGTCAGGCTGAAGAGATACTTGGAGATGAGGGGTGCTGATGGAGGACCTTGGAGTAGATTACGTGCCTTGCCTGCATTTTGGGTTGGTTTGTTGTATGATGAGGTTTCCCTACAAAATGTGATAGACATGATAGCAGACTGGACTGCTGAAGAAAGACAGATGCTGAGAGATCAGGTCCCCAAAACTGGCCTAAAGACACCGTTTCGTGGTAGATTGCTAAAGCATGTTGCTCAAGATGTGGTCCGGTTCGCAAAGGCTGGCCTGGAAAGAAGAGGCTTTAATGAAACAGGCTTCTTGAATGAGTTGGAGGAGGTAGCTAGAACTGGTGTAACACCAGCTGAGAAGCTACTGGAGTTGTATTACAGGAAATGGGGACAAAATATAGACCCTGTTTTTGAGGAGCTACTATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

485

Amino Acids

55.38

Weight (kDa)

5.7

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 9
AciI CCGC 1 cut(s) 77
AclWI GGATC 2 cut(s) 120, 822
AcsI RAATTY 3 cut(s) 446, 739, 792
AcuI CTGAAG 6 cut(s) 39, 417, 646, 774, 1070, 1209
AfaI GTAC 2 cut(s) 899, 934
AfiI CCNNNNNNNGG 3 cut(s) 602, 1226, 1247
AgsI TTSAA 4 cut(s) 374, 446, 875, 1339
AhdI GACNNNNNGTC 1 cut(s) 207
AjnI CCWGG 2 cut(s) 337, 1302
AjuI GAANNNNNNNTTGG 4 cut(s) 778, 810, 1043, 1075
AloI GAACNNNNNNTCC 2 cut(s) 1018, 1050
AluBI AGCT 6 cut(s) 728, 970, 1358, 1379, 1387, 1444
AluI AGCT 6 cut(s) 728, 970, 1358, 1379, 1387, 1444
Alw21I GWGCWC 1 cut(s) 367
Alw44I GTGCAC 1 cut(s) 363
AlwI GGATC 2 cut(s) 120, 822
AlwNI CAGNNNCTG 1 cut(s) 1204
AoxI GGCC 2 cut(s) 1228, 1300
ApaLI GTGCAC 1 cut(s) 363
ApeKI GCWGC 3 cut(s) 56, 89, 712
ApoI RAATTY 3 cut(s) 446, 739, 792
ArsI GACNNNNNNTTYG 2 cut(s) 1418, 1450
AspS9I GGNCC 3 cut(s) 1085, 1216, 1282
AsuC2I CCSGG 2 cut(s) 950, 984
AsuHPI GGTGA 2 cut(s) 735, 998
AvaII GGWCC 3 cut(s) 1085, 1216, 1282
AxyI CCTNAGG 1 cut(s) 1038
BaeGI GKGCMC 1 cut(s) 367
Bbv12I GWGCWC 1 cut(s) 367
BbvI GCAGC 3 cut(s) 43, 76, 724
BccI CCATC 1 cut(s) 1073
BciT130I CCWGG 2 cut(s) 339, 1304
BcnI CCSGG 2 cut(s) 950, 984
BfaI CTAG 2 cut(s) 699, 1359
BfmI CTRYAG 1 cut(s) 90
BisI GCNGC 4 cut(s) 57, 78, 90, 713
BlsI GCNGC 4 cut(s) 58, 79, 91, 714
Bme1390I CCNGG 4 cut(s) 339, 950, 984, 1304
Bme18I GGWCC 3 cut(s) 1085, 1216, 1282
BmeRI GACNNNNNGTC 1 cut(s) 207
BmgT120I GGNCC 3 cut(s) 1085, 1216, 1282
BmiI GGNNCC 2 cut(s) 336, 1218
BmrFI CCNGG 4 cut(s) 339, 950, 984, 1304
BmrI ACTGGG 1 cut(s) 945
BmsI GCATC 5 cut(s) 128, 391, 546, 1005, 1191
BmuI ACTGGG 1 cut(s) 945
BpmI CTGGAG 2 cut(s) 360, 1412
BpuEI CTTGAG 1 cut(s) 1257
BpuMI CCSGG 2 cut(s) 950, 984
BsaAI YACGTR 1 cut(s) 1103
BsaJI CCNNGG 2 cut(s) 561, 1088
BsaWI WCCGGW 1 cut(s) 1284
Bsc4I CCNNNNNNNGG 3 cut(s) 602, 1226, 1247
Bse1I ACTGG 6 cut(s) 826, 940, 1184, 1231, 1369, 1395
Bse21I CCTNAGG 1 cut(s) 1038
Bse3DI GCAATG 1 cut(s) 228
BseBI CCWGG 2 cut(s) 339, 1304
BseDI CCNNGG 2 cut(s) 561, 1088
BseGI GGATG 2 cut(s) 143, 945
BseLI CCNNNNNNNGG 3 cut(s) 602, 1226, 1247
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 4 cut(s) 162, 1029, 1196, 1371
BseNI ACTGG 6 cut(s) 826, 940, 1184, 1231, 1369, 1395
BseRI GAGGAG 2 cut(s) 1364, 1454
BseSI GKGCMC 1 cut(s) 367
BseXI GCAGC 3 cut(s) 43, 76, 724
BsgI GTGCAG 1 cut(s) 627
BshFI GGCC 2 cut(s) 1230, 1302
BsiHKAI GWGCWC 1 cut(s) 367
BsiSI CCGG 3 cut(s) 949, 983, 1285
BslFI GGGAC 2 cut(s) 1202, 1428
BslI CCNNNNNNNGG 3 cut(s) 602, 1226, 1247
BsmFI GGGAC 2 cut(s) 1202, 1428
BsmI GAATGC 1 cut(s) 978
BsnI GGCC 2 cut(s) 1230, 1302
Bsp1286I GDGCHC 1 cut(s) 367
Bsp143I GATC 4 cut(s) 112, 193, 814, 1210
BspACI CCGC 1 cut(s) 77
BspANI GGCC 2 cut(s) 1230, 1302
BspCNI CTCAG 4 cut(s) 163, 1030, 1197, 1372
BspLI GGNNCC 2 cut(s) 336, 1218
BspMAI CTGCAG 1 cut(s) 94
BspPI GGATC 2 cut(s) 120, 822
BsrDI GCAATG 1 cut(s) 228
BsrI ACTGG 6 cut(s) 826, 940, 1184, 1231, 1369, 1395
BssECI CCNNGG 2 cut(s) 561, 1088
BssMI GATC 4 cut(s) 112, 193, 814, 1210
BssT1I CCWWGG 2 cut(s) 561, 1088
Bst2UI CCWGG 2 cut(s) 339, 1304
Bst4CI ACNGT 2 cut(s) 932, 1242
Bst6I CTCTTC 2 cut(s) 1046, 1306
BstBAI YACGTR 1 cut(s) 1103
BstC8I GCNNGC 5 cut(s) 61, 143, 608, 1113, 1300
BstDEI CTNAG 5 cut(s) 171, 776, 1038, 1205, 1380
BstF5I GGATG 2 cut(s) 143, 945
BstKTI GATC 4 cut(s) 115, 196, 817, 1213
BstMBI GATC 4 cut(s) 112, 193, 814, 1210
BstMWI GCNNNNNNNGC 5 cut(s) 53, 86, 704, 734, 1112
BstNI CCWGG 2 cut(s) 339, 1304
BstNSI RCATGY 4 cut(s) 8, 456, 595, 1268
BstSCI CCNGG 4 cut(s) 337, 948, 982, 1302
BstSFI CTRYAG 1 cut(s) 90
BstSLI GKGCMC 1 cut(s) 367
BstV1I GCAGC 3 cut(s) 43, 76, 724
BstX2I RGATCY 1 cut(s) 112
BstYI RGATCY 1 cut(s) 112
Bsu36I CCTNAGG 1 cut(s) 1038
BsuRI GGCC 2 cut(s) 1230, 1302
BtsCI GGATG 2 cut(s) 143, 945
BtsI GCAGTG 1 cut(s) 639
BtsIMutI CAGTG 6 cut(s) 98, 168, 430, 639, 750, 819
Cac8I GCNNGC 5 cut(s) 61, 143, 608, 1113, 1300
CaiI CAGNNNCTG 1 cut(s) 1204
Cfr13I GGNCC 3 cut(s) 1085, 1216, 1282
Csp6I GTAC 2 cut(s) 898, 933
CviQI GTAC 2 cut(s) 898, 933
DdeI CTNAG 5 cut(s) 171, 776, 1038, 1205, 1380
DpnI GATC 4 cut(s) 114, 195, 816, 1212
DpnII GATC 4 cut(s) 112, 193, 814, 1210
DriI GACNNNNNGTC 1 cut(s) 207
Eam1104I CTCTTC 2 cut(s) 1046, 1306
Eam1105I GACNNNNNGTC 1 cut(s) 207
EarI CTCTTC 2 cut(s) 1046, 1306
Eco130I CCWWGG 2 cut(s) 561, 1088
Eco47I GGWCC 3 cut(s) 1085, 1216, 1282
Eco57I CTGAAG 6 cut(s) 39, 417, 646, 774, 1070, 1209
Eco81I CCTNAGG 1 cut(s) 1038
EcoO109I RGGNCCY 2 cut(s) 1085, 1216
EcoRI GAATTC 1 cut(s) 446
EcoRII CCWGG 2 cut(s) 337, 1302
EcoT14I CCWWGG 2 cut(s) 561, 1088
EcoT22I ATGCAT 2 cut(s) 6, 1020
ErhI CCWWGG 2 cut(s) 561, 1088
FalI AAGNNNNNCTT 4 cut(s) 99, 131, 1043, 1075
FaqI GGGAC 2 cut(s) 1202, 1428
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 4 cut(s) 57, 78, 90, 713
FokI GGATG 2 cut(s) 150, 952
Fsp4HI GCNGC 4 cut(s) 57, 78, 90, 713
FspBI CTAG 2 cut(s) 699, 1359
GluI GCNGC 4 cut(s) 57, 78, 90, 713
GsuI CTGGAG 2 cut(s) 360, 1412
HaeIII GGCC 2 cut(s) 1230, 1302
HapII CCGG 3 cut(s) 949, 983, 1285
HindIII AAGCTT 1 cut(s) 968
HinfI GANTC 3 cut(s) 498, 615, 829
HpaII CCGG 3 cut(s) 949, 983, 1285
HphI GGTGA 2 cut(s) 735, 998
Hpy166II GTNNAC 2 cut(s) 10, 365
Hpy188I TCNGA 4 cut(s) 503, 614, 670, 912
Hpy188III TCNNGA 9 cut(s) 135, 371, 631, 656, 862, 884, 1037, 1274, 1336
Hpy8I GTNNAC 2 cut(s) 10, 365
Hpy99I CGWCG 2 cut(s) 39, 42
HpyAV CCTTC 3 cut(s) 278, 749, 955
HpyCH4III ACNGT 2 cut(s) 932, 1242
HpyCH4IV ACGT 3 cut(s) 40, 208, 1102
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 86, 704, 734, 1112
HpyF3I CTNAG 5 cut(s) 171, 776, 1038, 1205, 1380
HpySE526I ACGT 3 cut(s) 40, 208, 1102
Kzo9I GATC 4 cut(s) 112, 193, 814, 1210
LmnI GCTCC 2 cut(s) 334, 1441
Lsp1109I GCAGC 3 cut(s) 43, 76, 724
LweI GCATC 5 cut(s) 128, 391, 546, 1005, 1191
MaeI CTAG 2 cut(s) 699, 1359
MaeII ACGT 3 cut(s) 40, 208, 1102
MaeIII GTNAC 1 cut(s) 1369
MalI GATC 4 cut(s) 114, 195, 816, 1212
MboI GATC 4 cut(s) 112, 193, 814, 1210
MboII GAAGA 3 cut(s) 1063, 1202, 1323
MflI RGATCY 1 cut(s) 112
MhlI GDGCHC 1 cut(s) 367
MlyI GAGTC 1 cut(s) 609
MmeI TCCRAC 1 cut(s) 1326
Mph1103I ATGCAT 2 cut(s) 6, 1020
MseI TTAA 5 cut(s) 243, 474, 540, 651, 1320
MslI CAYNNNNRTG 2 cut(s) 360, 824
MspA1I CMGCKG 1 cut(s) 1379
MspI CCGG 3 cut(s) 949, 983, 1285
MspR9I CCNGG 4 cut(s) 339, 950, 984, 1304
Mva1269I GAATGC 1 cut(s) 978
MvaI CCWGG 2 cut(s) 339, 1304
MwoI GCNNNNNNNGC 5 cut(s) 53, 86, 704, 734, 1112
NciI CCSGG 2 cut(s) 950, 984
NdeII GATC 4 cut(s) 112, 193, 814, 1210
NlaIV GGNNCC 2 cut(s) 336, 1218
NmeAIII GCCGAG 1 cut(s) 259
NsiI ATGCAT 2 cut(s) 6, 1020
NspI RCATGY 4 cut(s) 8, 456, 595, 1268
PctI GAATGC 1 cut(s) 978
PfeI GAWTC 2 cut(s) 498, 829
PfoI TCCNGGA 1 cut(s) 948
PkrI GCNGC 4 cut(s) 58, 79, 91, 714
PleI GAGTC 1 cut(s) 609
PpsI GAGTC 1 cut(s) 609
Ppu21I YACGTR 1 cut(s) 1103
PpuMI RGGWCCY 2 cut(s) 1085, 1216
Psp5II RGGWCCY 2 cut(s) 1085, 1216
Psp6I CCWGG 2 cut(s) 337, 1302
PspGI CCWGG 2 cut(s) 337, 1302
PspN4I GGNNCC 2 cut(s) 336, 1218
PspPI GGNCC 3 cut(s) 1085, 1216, 1282
PspPPI RGGWCCY 2 cut(s) 1085, 1216
PstI CTGCAG 1 cut(s) 94
PstNI CAGNNNCTG 1 cut(s) 1204
PsuI RGATCY 1 cut(s) 112
PvuII CAGCTG 1 cut(s) 1379
RsaI GTAC 2 cut(s) 899, 934
RsaNI GTAC 2 cut(s) 898, 933
RseI CAYNNNNRTG 2 cut(s) 360, 824
SaqAI TTAA 5 cut(s) 243, 474, 540, 651, 1320
SatI GCNGC 4 cut(s) 57, 78, 90, 713
Sau3AI GATC 4 cut(s) 112, 193, 814, 1210
Sau96I GGNCC 3 cut(s) 1085, 1216, 1282
SchI GAGTC 1 cut(s) 609
ScrFI CCNGG 4 cut(s) 339, 950, 984, 1304
SduI GDGCHC 1 cut(s) 367
SfaNI GCATC 5 cut(s) 128, 391, 546, 1005, 1191
SfcI CTRYAG 1 cut(s) 90
SinI GGWCC 3 cut(s) 1085, 1216, 1282
SmiMI CAYNNNNRTG 2 cut(s) 360, 824
SmlI CTYRAG 1 cut(s) 1272
SmoI CTYRAG 1 cut(s) 1272
SsiI CCGC 1 cut(s) 77
SspMI CTAG 2 cut(s) 699, 1359
StyD4I CCNGG 4 cut(s) 337, 948, 982, 1302
StyI CCWWGG 2 cut(s) 561, 1088
TaaI ACNGT 2 cut(s) 932, 1242
TaiI ACGT 3 cut(s) 43, 211, 1105
TaqI TCGA 5 cut(s) 351, 618, 843, 861, 927
TatI WGTACW 2 cut(s) 897, 932
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 2 cut(s) 498, 829
Tru1I TTAA 5 cut(s) 243, 474, 540, 651, 1320
Tru9I TTAA 5 cut(s) 243, 474, 540, 651, 1320
TscAI CASTG 6 cut(s) 105, 175, 430, 646, 757, 826
TseI GCWGC 3 cut(s) 56, 89, 712
TspDTI ATGAA 5 cut(s) 230, 237, 946, 1016, 1338
TspRI CASTG 6 cut(s) 105, 175, 430, 646, 757, 826
VneI GTGCAC 1 cut(s) 363
VpaK11BI GGWCC 3 cut(s) 1085, 1216, 1282
XapI RAATTY 3 cut(s) 446, 739, 792
XceI RCATGY 4 cut(s) 8, 456, 595, 1268
XmiI GTMKAC 1 cut(s) 9
XspI CTAG 2 cut(s) 699, 1359
Zsp2I ATGCAT 2 cut(s) 6, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.