RLG00000029805

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001519
Physical Location & Seq
Forward (+)
49508360 .. 49514668
6309 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029805

Sequence Viewer

Length: 1089 bp
ATGGCGTACCTATCCAAAATCCAGAGAGGCGACCATATCCAGCCGTTCGGGTGCATGATTGCGGTGGAAGAGTCCACCTTCGGGGTCATTGCCTACAGCGAGAATGCCCGGGACCTGCTCGAGCTGATGCCCCAGTCTGTTCCGGTGCTCGAGAGGCGGAAGATCCTCACAATCGGCTTGACATCCTCATGGCAGAGGCATGCCCGGCAACCGCTACTACACCGTCGTGTTCCAGCCCCACGACCGCATCATAGGCTCCCCTCCGGCGGTCACTTGACCCATGAGTGGTGGTGGCTCCGAAATACGCCGACAAGACGTGGAAGTCACATCTATACTGATACTGACAAGAATCGCACTGGCATGCTTCCTTTTGCCTTTGATAACTCCTTTGGGTTTGAGCGGTATGTTGATTATTCTCTTGATGTTCCCATGTACTATGTTTATCGGCAAAAGAAGTGTATTGAATGTACTGGAGTGACCTTTCGGGACTTTATGGCTGGAAAGCTTCATTGCTTTCCGGGTGAATTACCAACCCTGAATGATTGGGAAAATCATTTGGGAATTCTATATCCAGAGGTCAGGCTGAAGAGATACTTAGAGATGAGGGGTGCTGATGGAGGGCCTTGGAGGAGCTTATGTGCTTTGCCTGCATTTTGGGTGGGCTTACTGTATGATGAGGTTTCCCTGCATAATGTGTTAGACATGACAGCTGATTGGACTCCCGAAGAAAGACAGATGCTGAGAGATAAGGTTCCTAAAACGGGTCTAAAGACACCATTTCGTGATGGATTACTCAAGTATGTTGCCGAAGATGTAGTAAAGTTTGCAAAGGATGGCCTGGAAAGAAGAGGCATTAAGGAATCGGGGTTCTTAAATGAAGCGGTCGAGGTTGTGAGAACAGGTGTAACACCAGCTGAGAAGCTATTGGAGTTGTATAATGGGAAGTGGAAACAACAGATAGACCAATTTTTGAAGAGCTACTATGCTGAACTGAAGTTGGGCACTTCAGCAGAGGCATTGGATTTTAGGCAGATGATCTTTCAGTTTTCCAGTACCCAGGTTATGTCATGGAAGGTTAAGGATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

363

Amino Acids

42.04

Weight (kDa)

8.79

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 2 - 47 1.1e-13 PAS fold
GCS2 PF04107 108 - 322 3.2e-38 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 321
Acc36I ACCTGC 1 cut(s) 123
AccBSI CCGCTC 1 cut(s) 400
AciI CCGC 7 cut(s) 62, 157, 212, 245, 267, 400, 881
AclWI GGATC 1 cut(s) 157
AcsI RAATTY 1 cut(s) 561
AcuI CTGAAG 3 cut(s) 605, 990, 1013
AfaI GTAC 4 cut(s) 8, 434, 469, 1054
AfiI CCNNNNNNNGG 4 cut(s) 81, 266, 285, 761
AgsI TTSAA 2 cut(s) 464, 973
AjiI CACGTC 1 cut(s) 317
AjnI CCWGG 2 cut(s) 837, 1056
AleI CACNNNNGTG 1 cut(s) 225
AloI GAACNNNNNNTCC 2 cut(s) 851, 883
AluBI AGCT 7 cut(s) 124, 505, 633, 710, 914, 922, 978
AluI AGCT 7 cut(s) 124, 505, 633, 710, 914, 922, 978
Alw21I GWGCWC 1 cut(s) 150
AlwI GGATC 1 cut(s) 157
AlwNI CAGNNNCTG 1 cut(s) 739
Ama87I CYCGRG 3 cut(s) 108, 119, 149
AoxI GGCC 2 cut(s) 620, 835
ApoI RAATTY 1 cut(s) 561
AspS9I GGNCC 2 cut(s) 112, 620
AsuC2I CCSGG 4 cut(s) 109, 110, 205, 519
AsuHPI GGTGA 1 cut(s) 533
AvaI CYCGRG 3 cut(s) 108, 119, 149
AvaII GGWCC 1 cut(s) 112
BaeGI GKGCMC 1 cut(s) 1004
Bbv12I GWGCWC 1 cut(s) 150
BccI CCATC 3 cut(s) 608, 779, 827
BceAI ACGGC 1 cut(s) 28
BciT130I CCWGG 2 cut(s) 839, 1058
BcnI CCSGG 4 cut(s) 109, 110, 205, 519
BfmI CTRYAG 1 cut(s) 94
BfuAI ACCTGC 1 cut(s) 123
Bme1390I CCNGG 6 cut(s) 109, 110, 205, 519, 839, 1058
Bme18I GGWCC 1 cut(s) 112
BmeT110I CYCGRG 3 cut(s) 108, 119, 149
BmgBI CACGTC 1 cut(s) 317
BmgT120I GGNCC 2 cut(s) 112, 620
BmiI GGNNCC 4 cut(s) 113, 257, 296, 753
BmrFI CCNGG 6 cut(s) 109, 110, 205, 519, 839, 1058
BmrI ACTGGG 1 cut(s) 127
BmsI GCATC 3 cut(s) 117, 256, 726
BmuI ACTGGG 1 cut(s) 127
BpmI CTGGAG 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 779
BpuMI CCSGG 4 cut(s) 109, 110, 205, 519
BsaJI CCNNGG 3 cut(s) 108, 623, 1056
BsaWI WCCGGW 1 cut(s) 142
BsaXI ACNNNNNCTCC 2 cut(s) 622, 652
Bsc4I CCNNNNNNNGG 4 cut(s) 81, 266, 285, 761
Bse1I ACTGG 4 cut(s) 133, 361, 475, 1050
Bse3DI GCAATG 2 cut(s) 87, 508
BseBI CCWGG 2 cut(s) 839, 1058
BseDI CCNNGG 3 cut(s) 108, 623, 1056
BseGI GGATG 2 cut(s) 182, 838
BseLI CCNNNNNNNGG 4 cut(s) 81, 266, 285, 761
BseMI GCAATG 2 cut(s) 87, 508
BseMII CTCAG 2 cut(s) 731, 906
BseNI ACTGG 4 cut(s) 133, 361, 475, 1050
BseRI GAGGAG 1 cut(s) 643
BseSI GKGCMC 1 cut(s) 1004
Bsh1285I CGRYCG 2 cut(s) 245, 885
BshFI GGCC 2 cut(s) 622, 837
BsiEI CGRYCG 2 cut(s) 245, 885
BsiHKAI GWGCWC 1 cut(s) 150
BsiHKCI CYCGRG 3 cut(s) 108, 119, 149
BsiSI CCGG 5 cut(s) 109, 143, 205, 264, 518
BslFI GGGAC 2 cut(s) 125, 500
BslI CCNNNNNNNGG 4 cut(s) 81, 266, 285, 761
BsmFI GGGAC 2 cut(s) 125, 500
BsmI GAATGC 1 cut(s) 109
BsnI GGCC 2 cut(s) 622, 837
BsoBI CYCGRG 3 cut(s) 108, 119, 149
Bsp1286I GDGCHC 2 cut(s) 150, 1004
Bsp143I GATC 2 cut(s) 162, 1035
BspACI CCGC 7 cut(s) 62, 157, 212, 245, 267, 400, 881
BspANI GGCC 2 cut(s) 622, 837
BspCNI CTCAG 2 cut(s) 732, 907
BspLI GGNNCC 4 cut(s) 113, 257, 296, 753
BspMI ACCTGC 1 cut(s) 123
BspPI GGATC 1 cut(s) 157
BspQI GCTCTTC 1 cut(s) 968
BsrBI CCGCTC 1 cut(s) 400
BsrDI GCAATG 2 cut(s) 87, 508
BsrI ACTGG 4 cut(s) 133, 361, 475, 1050
BssECI CCNNGG 3 cut(s) 108, 623, 1056
BssMI GATC 2 cut(s) 162, 1035
BssT1I CCWWGG 1 cut(s) 623
Bst2UI CCWGG 2 cut(s) 839, 1058
Bst4CI ACNGT 2 cut(s) 224, 669
Bst6I CTCTTC 4 cut(s) 63, 581, 841, 968
BstC8I GCNNGC 3 cut(s) 201, 362, 648
BstDEI CTNAG 3 cut(s) 595, 740, 915
BstF5I GGATG 2 cut(s) 182, 838
BstKTI GATC 2 cut(s) 165, 1038
BstMBI GATC 2 cut(s) 162, 1035
BstMCI CGRYCG 2 cut(s) 245, 885
BstMWI GCNNNNNNNGC 4 cut(s) 154, 205, 253, 647
BstNI CCWGG 2 cut(s) 839, 1058
BstNSI RCATGY 2 cut(s) 203, 364
BstSCI CCNGG 6 cut(s) 107, 108, 203, 517, 837, 1056
BstSFI CTRYAG 1 cut(s) 94
BstSLI GKGCMC 1 cut(s) 1004
BstX2I RGATCY 1 cut(s) 162
BstYI RGATCY 1 cut(s) 162
BsuRI GGCC 2 cut(s) 622, 837
BtrI CACGTC 1 cut(s) 317
BtsCI GGATG 2 cut(s) 182, 838
BtsIMutI CAGTG 1 cut(s) 354
BveI ACCTGC 1 cut(s) 123
Cac8I GCNNGC 3 cut(s) 201, 362, 648
CaiI CAGNNNCTG 1 cut(s) 739
Cfr13I GGNCC 2 cut(s) 112, 620
Cfr9I CCCGGG 1 cut(s) 108
Csp6I GTAC 4 cut(s) 7, 433, 468, 1053
CviAII CATG 8 cut(s) 55, 189, 200, 281, 361, 430, 703, 1068
CviQI GTAC 4 cut(s) 7, 433, 468, 1053
DdeI CTNAG 3 cut(s) 595, 740, 915
DpnI GATC 2 cut(s) 164, 1037
DpnII GATC 2 cut(s) 162, 1035
DrdI GACNNNNNNGTC 1 cut(s) 321
DseDI GACNNNNNNGTC 1 cut(s) 321
Eam1104I CTCTTC 4 cut(s) 63, 581, 841, 968
EarI CTCTTC 4 cut(s) 63, 581, 841, 968
EciI GGCGGA 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 623
Eco47I GGWCC 1 cut(s) 112
Eco57I CTGAAG 3 cut(s) 605, 990, 1013
Eco88I CYCGRG 3 cut(s) 108, 119, 149
EcoO109I RGGNCCY 2 cut(s) 112, 620
EcoRI GAATTC 1 cut(s) 561
EcoRII CCWGG 2 cut(s) 837, 1056
EcoT14I CCWWGG 1 cut(s) 623
ErhI CCWWGG 1 cut(s) 623
FaeI CATG 8 cut(s) 58, 192, 203, 284, 364, 433, 706, 1071
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FaqI GGGAC 2 cut(s) 125, 500
FatI CATG 8 cut(s) 54, 188, 199, 280, 360, 429, 702, 1067
FokI GGATG 2 cut(s) 169, 845
GsuI CTGGAG 1 cut(s) 492
HaeIII GGCC 2 cut(s) 622, 837
HapII CCGG 5 cut(s) 109, 143, 205, 264, 518
Hin1II CATG 8 cut(s) 58, 192, 203, 284, 364, 433, 706, 1071
HindIII AAGCTT 1 cut(s) 503
HinfI GANTC 4 cut(s) 71, 349, 718, 860
HpaII CCGG 5 cut(s) 109, 143, 205, 264, 518
HphI GGTGA 1 cut(s) 533
Hpy166II GTNNAC 1 cut(s) 75
Hpy188I TCNGA 1 cut(s) 299
Hpy188III TCNNGA 7 cut(s) 22, 151, 419, 485, 572, 722, 782
Hpy8I GTNNAC 1 cut(s) 75
Hpy99I CGWCG 1 cut(s) 228
HpyAV CCTTC 2 cut(s) 88, 1066
HpyCH4III ACNGT 2 cut(s) 224, 669
HpyCH4IV ACGT 1 cut(s) 316
HpyCH4V TGCA 4 cut(s) 54, 650, 688, 827
HpyF10VI GCNNNNNNNGC 4 cut(s) 154, 205, 253, 647
HpyF3I CTNAG 3 cut(s) 595, 740, 915
HpySE526I ACGT 1 cut(s) 316
Hsp92II CATG 8 cut(s) 58, 192, 203, 284, 364, 433, 706, 1071
Kzo9I GATC 2 cut(s) 162, 1035
LguI GCTCTTC 1 cut(s) 968
LmnI GCTCC 3 cut(s) 261, 300, 630
LweI GCATC 3 cut(s) 117, 256, 726
MaeII ACGT 1 cut(s) 316
MaeIII GTNAC 4 cut(s) 269, 323, 475, 904
MalI GATC 2 cut(s) 164, 1037
MbiI CCGCTC 1 cut(s) 400
MboI GATC 2 cut(s) 162, 1035
MboII GAAGA 7 cut(s) 80, 172, 598, 737, 821, 858, 985
MflI RGATCY 1 cut(s) 162
MhlI GDGCHC 2 cut(s) 150, 1004
MluCI AATT 4 cut(s) 524, 561, 965, 1084
MlyI GAGTC 2 cut(s) 80, 712
MseI TTAA 3 cut(s) 855, 872, 1077
MslI CAYNNNNRTG 3 cut(s) 187, 225, 359
MspA1I CMGCKG 2 cut(s) 710, 914
MspI CCGG 5 cut(s) 109, 143, 205, 264, 518
MspR9I CCNGG 6 cut(s) 109, 110, 205, 519, 839, 1058
Mva1269I GAATGC 1 cut(s) 109
MvaI CCWGG 2 cut(s) 839, 1058
MwoI GCNNNNNNNGC 4 cut(s) 154, 205, 253, 647
NciI CCSGG 4 cut(s) 109, 110, 205, 519
NdeII GATC 2 cut(s) 162, 1035
NlaIII CATG 8 cut(s) 58, 192, 203, 284, 364, 433, 706, 1071
NlaIV GGNNCC 4 cut(s) 113, 257, 296, 753
NmuCI GTSAC 3 cut(s) 269, 323, 475
NspI RCATGY 2 cut(s) 203, 364
OliI CACNNNNGTG 1 cut(s) 225
PaeI GCATGC 2 cut(s) 203, 364
PaeR7I CTCGAG 2 cut(s) 119, 149
PciSI GCTCTTC 1 cut(s) 968
PctI GAATGC 1 cut(s) 109
PfeI GAWTC 2 cut(s) 349, 860
PleI GAGTC 2 cut(s) 79, 712
PpsI GAGTC 2 cut(s) 79, 712
PpuMI RGGWCCY 1 cut(s) 112
Psp5II RGGWCCY 1 cut(s) 112
Psp6I CCWGG 2 cut(s) 837, 1056
PspGI CCWGG 2 cut(s) 837, 1056
PspN4I GGNNCC 4 cut(s) 113, 257, 296, 753
PspPI GGNCC 2 cut(s) 112, 620
PspPPI RGGWCCY 1 cut(s) 112
PspXI VCTCGAGB 1 cut(s) 119
PstNI CAGNNNCTG 1 cut(s) 739
PsuI RGATCY 1 cut(s) 162
PvuII CAGCTG 2 cut(s) 710, 914
RsaI GTAC 4 cut(s) 8, 434, 469, 1054
RsaNI GTAC 4 cut(s) 7, 433, 468, 1053
RseI CAYNNNNRTG 3 cut(s) 187, 225, 359
SapI GCTCTTC 1 cut(s) 968
SaqAI TTAA 3 cut(s) 855, 872, 1077
Sau3AI GATC 2 cut(s) 162, 1035
Sau96I GGNCC 2 cut(s) 112, 620
SchI GAGTC 2 cut(s) 80, 712
ScrFI CCNGG 6 cut(s) 109, 110, 205, 519, 839, 1058
SduI GDGCHC 2 cut(s) 150, 1004
SfaNI GCATC 3 cut(s) 117, 256, 726
SfcI CTRYAG 1 cut(s) 94
Sfr274I CTCGAG 2 cut(s) 119, 149
SinI GGWCC 1 cut(s) 112
SlaI CTCGAG 2 cut(s) 119, 149
SmaI CCCGGG 1 cut(s) 110
SmiMI CAYNNNNRTG 3 cut(s) 187, 225, 359
SmlI CTYRAG 3 cut(s) 119, 149, 794
SmoI CTYRAG 3 cut(s) 119, 149, 794
SphI GCATGC 2 cut(s) 203, 364
Sse9I AATT 4 cut(s) 524, 561, 965, 1084
SsiI CCGC 7 cut(s) 62, 157, 212, 245, 267, 400, 881
StyD4I CCNGG 6 cut(s) 107, 108, 203, 517, 837, 1056
StyI CCWWGG 1 cut(s) 623
TaaI ACNGT 2 cut(s) 224, 669
TaiI ACGT 1 cut(s) 319
TaqI TCGA 3 cut(s) 120, 150, 885
TasI AATT 4 cut(s) 524, 561, 965, 1084
TatI WGTACW 2 cut(s) 432, 467
TfiI GAWTC 2 cut(s) 349, 860
Tru1I TTAA 3 cut(s) 855, 872, 1077
Tru9I TTAA 3 cut(s) 855, 872, 1077
TscAI CASTG 1 cut(s) 361
TseFI GTSAC 3 cut(s) 269, 323, 475
Tsp45I GTSAC 3 cut(s) 269, 323, 475
TspDTI ATGAA 2 cut(s) 497, 891
TspMI CCCGGG 1 cut(s) 108
TspRI CASTG 1 cut(s) 361
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 1 cut(s) 561
XceI RCATGY 2 cut(s) 203, 364
XhoI CTCGAG 2 cut(s) 119, 149
XmaI CCCGGG 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.