Rroxscaffold_3G00258230

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001519
Physical Location & Seq
Reverse (-)
52541725 .. 52545569
3845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00258230.1

Sequence Viewer

Length: 1497 bp
ATGGCACTCATTTCCCAGACAAGTTCATCATACTACTGCAGAAGCATTGGCTTCTCTTCCTCTGCATTTTATTCTACCACTGCTAGGAACATACAGATTTTGCATACAGACAATGTTGCAGCACAGAAACAAGGACGAAAGAGTGCGATAGTTGCTACAAGTCCTATCACAGAAGATGCAGTAGTAGCTACAATGCTGCTCACAAAAGAAGATCTTGTAGGCCATCTTGCCTCTGGATGCAAGCCTAAAGAGAAATGGAGAATTGGCGTGGAACATGAGAGGTTTGGCTTCGATTTAAAAAGTTTACGTCCCATGCAATATCAACAAATAGCTGAGCTGCTAAATGGGATTGCTGAGAGATTTAAGTGGGAGGAAATCATGGAAGGTGATAACACTATTGGACTCAAACAGGGTAAGCAGAGTATATCACTTGAGCCTGGAGGTCAGTTTGAGCTGAGTGGTGCACCTCTTGAAACTCTGCATCAAGTTTGTACGGAAGTCGATTCACTACTCTGTCAGGTTAAAGCAGTTGCAGAGAAAATGGGAATCGGATTTCTTGATATTGGTGGTCAGCCGAAATGGAGCAATCAAGACATACCTCTAATGCCCAGGGCATTATACGAGATTATTTATAATTACATGCCAAAAGTTGGCTCGCTTGGACATGATTTGATGTTCAAGACTAGCACTGTACAGGTCAATCTGGACTTCAGTTCTGAAGCAGACATGAAACGGAAACTTCGTGCTAGTCTTGCTTTGCAGCCTATAGCAACAGCCCTGTTTGCAAATTCACCTTTTACTGACGGAAAGCCAAATGGTTTTTTAAGCAGGAGAAGTCACATCTATACTGATACTGACAAGGATCGCACTGGCATTCTTCCTTTTGCTTTCGATGACTCCTTTGGGTTTGAGCGATATGTGGATTATGCTCTTGATGTTCCCATGTACTGTGTTTATCGGAAAAAGAAGTGTATCGACTGTACTGGAATGACCTTTCGGGACTTTATGGCCGGAAAGCTTCACTGCCTTCCGGGTGAATTACCGACCCTGAATGATTGGGAGAATCATTTGGGAACTTTATATCCAGAGGCTGAAGAGATACTTAGAGATGAGGGGGCTGATGGAGGGCCTTGGAGGCGGTTATGTGCTTTGCCTGCATTTTGGGTGGGTTTACTGTATGATGAGGTTTCCCTGCAGAATGTGTTAGATATGATAGCTGATTGGACTCCTGAAGAAAGACAGATGCTGAGAGATAAGGTTCCGAAAACGGGTCTAAAGACACCATTTCGTGATGGATTACTCAAGCAAGTTGCCGAAGATGTAGTCCAGTTTGCAAAGGATGGCCTGGAAAGAAGAGGCTTTAAGGAATCAGGGTTCTTAAATGAAGCGGTGGAGATTGTGAGAACAGGTGTAACACCAGCTGAGAAGCTATTGGAGTTGTATAATGGAAAGTGGAAACAACAGATAGACCCAGTTTTTGAAGAGCTACTATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

498

Amino Acids

56.05

Weight (kDa)

5.42

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 87 - 495 4.7e-85 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 633
AccB7I CCANNNNNTGG 1 cut(s) 650
AciI CCGC 2 cut(s) 1138, 1388
AclWI GGATC 1 cut(s) 870
AcoI YGGCCR 1 cut(s) 1008
AcsI RAATTY 1 cut(s) 787
AcuI CTGAAG 4 cut(s) 694, 738, 1113, 1251
AfaI GTAC 4 cut(s) 493, 693, 947, 982
AfiI CCNNNNNNNGG 3 cut(s) 84, 650, 1268
AgsI TTSAA 3 cut(s) 473, 679, 1481
AjnI CCWGG 3 cut(s) 436, 608, 1344
AloI GAACNNNNNNTCC 4 cut(s) 1066, 1098, 1358, 1390
AluBI AGCT 9 cut(s) 188, 332, 337, 454, 1018, 1217, 1421, 1429, 1486
AluI AGCT 9 cut(s) 188, 332, 337, 454, 1018, 1217, 1421, 1429, 1486
Alw21I GWGCWC 1 cut(s) 466
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 1 cut(s) 870
AlwNI CAGNNNCTG 2 cut(s) 1091, 1246
AoxI GGCC 4 cut(s) 220, 1008, 1127, 1342
ApaLI GTGCAC 1 cut(s) 462
ApeKI GCWGC 4 cut(s) 119, 196, 337, 760
ApoI RAATTY 1 cut(s) 787
ArsI GACNNNNNNTTYG 4 cut(s) 1308, 1340, 1460, 1492
AspS9I GGNCC 1 cut(s) 1127
AsuC2I CCSGG 1 cut(s) 1032
AsuHPI GGTGA 3 cut(s) 398, 783, 1046
BaeGI GKGCMC 1 cut(s) 466
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 4 cut(s) 131, 183, 324, 772
BccI CCATC 4 cut(s) 231, 1115, 1286, 1334
BciT130I CCWGG 3 cut(s) 438, 610, 1346
BcnI CCSGG 1 cut(s) 1032
BfaI CTAG 3 cut(s) 84, 684, 747
BfmI CTRYAG 3 cut(s) 37, 765, 1193
BglI GCCNNNNNGGC 1 cut(s) 1135
BglII AGATCT 1 cut(s) 211
BisI GCNGC 4 cut(s) 120, 197, 338, 761
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 4 cut(s) 121, 198, 339, 762
Bme1390I CCNGG 4 cut(s) 438, 610, 1032, 1346
BmgT120I GGNCC 1 cut(s) 1127
BmiI GGNNCC 1 cut(s) 1260
BmrFI CCNGG 4 cut(s) 438, 610, 1032, 1346
BmrI ACTGGG 1 cut(s) 1466
BmsI GCATC 4 cut(s) 166, 227, 490, 1233
BmuI ACTGGG 1 cut(s) 1466
BpmI CTGGAG 1 cut(s) 459
Bpu1102I GCTNAGC 1 cut(s) 333
BpuEI CTTGAG 2 cut(s) 452, 1286
BpuMI CCSGG 1 cut(s) 1032
BsaJI CCNNGG 3 cut(s) 608, 609, 1130
Bsc4I CCNNNNNNNGG 3 cut(s) 84, 650, 1268
Bse1I ACTGG 4 cut(s) 874, 988, 1327, 1472
BseBI CCWGG 3 cut(s) 438, 610, 1346
BseDI CCNNGG 3 cut(s) 608, 609, 1130
BseGI GGATG 2 cut(s) 242, 1345
BseLI CCNNNNNNNGG 3 cut(s) 84, 650, 1268
BseMII CTCAG 5 cut(s) 324, 345, 446, 1238, 1413
BseNI ACTGG 4 cut(s) 874, 988, 1327, 1472
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 4 cut(s) 131, 183, 324, 772
BshFI GGCC 4 cut(s) 222, 1010, 1129, 1344
BsiHKAI GWGCWC 1 cut(s) 466
BsiSI CCGG 2 cut(s) 1011, 1031
BslFI GGGAC 2 cut(s) 294, 1013
BslI CCNNNNNNNGG 3 cut(s) 84, 650, 1268
BsmFI GGGAC 2 cut(s) 294, 1013
BsmI GAATGC 1 cut(s) 873
BsnI GGCC 4 cut(s) 222, 1010, 1129, 1344
Bsp1286I GDGCHC 1 cut(s) 466
Bsp1407I TGTACA 1 cut(s) 691
Bsp143I GATC 2 cut(s) 211, 862
Bsp1720I GCTNAGC 1 cut(s) 333
BspACI CCGC 2 cut(s) 1138, 1388
BspANI GGCC 4 cut(s) 222, 1010, 1129, 1344
BspCNI CTCAG 5 cut(s) 325, 346, 447, 1239, 1414
BspLI GGNNCC 1 cut(s) 1260
BspMAI CTGCAG 2 cut(s) 41, 1197
BspPI GGATC 1 cut(s) 870
BspQI GCTCTTC 1 cut(s) 1476
BsrGI TGTACA 1 cut(s) 691
BsrI ACTGG 4 cut(s) 874, 988, 1327, 1472
BssECI CCNNGG 3 cut(s) 608, 609, 1130
BssMI GATC 2 cut(s) 211, 862
BssT1I CCWWGG 1 cut(s) 1130
Bst2UI CCWGG 3 cut(s) 438, 610, 1346
Bst4CI ACNGT 4 cut(s) 691, 950, 980, 1176
Bst6I CTCTTC 4 cut(s) 61, 1089, 1348, 1476
BstAUI TGTACA 1 cut(s) 691
BstC8I GCNNGC 3 cut(s) 242, 656, 1155
BstDEI CTNAG 6 cut(s) 333, 354, 455, 1103, 1247, 1422
BstF5I GGATG 2 cut(s) 242, 1345
BstKTI GATC 2 cut(s) 214, 865
BstMBI GATC 2 cut(s) 211, 862
BstMWI GCNNNNNNNGC 6 cut(s) 152, 185, 752, 782, 1135, 1154
BstNI CCWGG 3 cut(s) 438, 610, 1346
BstNSI RCATGY 1 cut(s) 643
BstSCI CCNGG 4 cut(s) 436, 608, 1030, 1344
BstSFI CTRYAG 3 cut(s) 37, 765, 1193
BstSLI GKGCMC 1 cut(s) 466
BstV1I GCAGC 4 cut(s) 131, 183, 324, 772
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BsuRI GGCC 4 cut(s) 222, 1010, 1129, 1344
BtsCI GGATG 2 cut(s) 242, 1345
BtsI GCAGTG 2 cut(s) 78, 1021
BtsIMutI CAGTG 4 cut(s) 78, 687, 867, 1021
Cac8I GCNNGC 3 cut(s) 242, 656, 1155
CaiI CAGNNNCTG 2 cut(s) 1091, 1246
Cfr13I GGNCC 1 cut(s) 1127
Csp6I GTAC 4 cut(s) 492, 692, 946, 981
CviAII CATG 7 cut(s) 275, 313, 379, 640, 665, 727, 943
CviQI GTAC 4 cut(s) 492, 692, 946, 981
DdeI CTNAG 6 cut(s) 333, 354, 455, 1103, 1247, 1422
DpnI GATC 2 cut(s) 213, 864
DpnII GATC 2 cut(s) 211, 862
DraI TTTAAA 1 cut(s) 297
EaeI YGGCCR 1 cut(s) 1008
Eam1104I CTCTTC 4 cut(s) 61, 1089, 1348, 1476
EarI CTCTTC 4 cut(s) 61, 1089, 1348, 1476
Eco130I CCWWGG 1 cut(s) 1130
Eco57I CTGAAG 4 cut(s) 694, 738, 1113, 1251
EcoO109I RGGNCCY 1 cut(s) 1127
EcoRII CCWGG 3 cut(s) 436, 608, 1344
EcoT14I CCWWGG 1 cut(s) 1130
ErhI CCWWGG 1 cut(s) 1130
FaeI CATG 7 cut(s) 278, 316, 382, 643, 668, 730, 946
FalI AAGNNNNNCTT 4 cut(s) 198, 230, 1086, 1118
FaqI GGGAC 2 cut(s) 294, 1013
FatI CATG 7 cut(s) 274, 312, 378, 639, 664, 726, 942
Fnu4HI GCNGC 4 cut(s) 120, 197, 338, 761
FokI GGATG 2 cut(s) 249, 1352
Fsp4HI GCNGC 4 cut(s) 120, 197, 338, 761
FspBI CTAG 3 cut(s) 84, 684, 747
GluI GCNGC 4 cut(s) 120, 197, 338, 761
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 4 cut(s) 222, 1010, 1129, 1344
HapII CCGG 2 cut(s) 1011, 1031
Hin1II CATG 7 cut(s) 278, 316, 382, 643, 668, 730, 946
HindIII AAGCTT 1 cut(s) 1016
HinfI GANTC 7 cut(s) 402, 503, 546, 896, 1063, 1225, 1367
HpaII CCGG 2 cut(s) 1011, 1031
HphI GGTGA 3 cut(s) 398, 783, 1046
Hpy166II GTNNAC 3 cut(s) 305, 464, 1172
Hpy188I TCNGA 4 cut(s) 551, 718, 960, 1263
Hpy8I GTNNAC 3 cut(s) 305, 464, 1172
HpyAV CCTTC 2 cut(s) 377, 1037
HpyCH4III ACNGT 4 cut(s) 691, 950, 980, 1176
HpyCH4IV ACGT 1 cut(s) 307
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 185, 752, 782, 1135, 1154
HpyF3I CTNAG 6 cut(s) 333, 354, 455, 1103, 1247, 1422
HpySE526I ACGT 1 cut(s) 307
Hsp92II CATG 7 cut(s) 278, 316, 382, 643, 668, 730, 946
Kzo9I GATC 2 cut(s) 211, 862
LguI GCTCTTC 1 cut(s) 1476
LmnI GCTCC 1 cut(s) 582
Lsp1109I GCAGC 4 cut(s) 131, 183, 324, 772
LweI GCATC 4 cut(s) 166, 227, 490, 1233
MaeI CTAG 3 cut(s) 84, 684, 747
MaeII ACGT 1 cut(s) 307
MaeIII GTNAC 2 cut(s) 836, 1411
MalI GATC 2 cut(s) 213, 864
MboI GATC 2 cut(s) 211, 862
MboII GAAGA 9 cut(s) 48, 185, 221, 869, 1106, 1244, 1328, 1365, 1493
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 1 cut(s) 466
MluCI AATT 4 cut(s) 261, 634, 787, 1037
MlyI GAGTC 3 cut(s) 396, 890, 1219
MseI TTAA 6 cut(s) 296, 363, 522, 824, 1362, 1379
MspA1I CMGCKG 1 cut(s) 1421
MspI CCGG 2 cut(s) 1011, 1031
MspR9I CCNGG 4 cut(s) 438, 610, 1032, 1346
Mva1269I GAATGC 1 cut(s) 873
MvaI CCWGG 3 cut(s) 438, 610, 1346
MwoI GCNNNNNNNGC 6 cut(s) 152, 185, 752, 782, 1135, 1154
NciI CCSGG 1 cut(s) 1032
NdeII GATC 2 cut(s) 211, 862
NlaIII CATG 7 cut(s) 278, 316, 382, 643, 668, 730, 946
NlaIV GGNNCC 1 cut(s) 1260
NmuCI GTSAC 1 cut(s) 836
NspI RCATGY 1 cut(s) 643
PasI CCCWGGG 1 cut(s) 609
PciSI GCTCTTC 1 cut(s) 1476
PcsI WCGNNNNNNNCGW 1 cut(s) 739
PctI GAATGC 1 cut(s) 873
PfeI GAWTC 4 cut(s) 503, 546, 1063, 1367
PflMI CCANNNNNTGG 1 cut(s) 650
PkrI GCNGC 4 cut(s) 121, 198, 339, 762
PleI GAGTC 3 cut(s) 396, 890, 1219
PpsI GAGTC 3 cut(s) 396, 890, 1219
PsiI TTATAA 1 cut(s) 633
Psp6I CCWGG 3 cut(s) 436, 608, 1344
PspGI CCWGG 3 cut(s) 436, 608, 1344
PspN4I GGNNCC 1 cut(s) 1260
PspPI GGNCC 1 cut(s) 1127
PstI CTGCAG 2 cut(s) 41, 1197
PstNI CAGNNNCTG 2 cut(s) 1091, 1246
PsuI RGATCY 1 cut(s) 211
PvuII CAGCTG 1 cut(s) 1421
RsaI GTAC 4 cut(s) 493, 693, 947, 982
RsaNI GTAC 4 cut(s) 492, 692, 946, 981
SapI GCTCTTC 1 cut(s) 1476
SaqAI TTAA 6 cut(s) 296, 363, 522, 824, 1362, 1379
SatI GCNGC 4 cut(s) 120, 197, 338, 761
Sau3AI GATC 2 cut(s) 211, 862
Sau96I GGNCC 1 cut(s) 1127
SchI GAGTC 3 cut(s) 396, 890, 1219
ScrFI CCNGG 4 cut(s) 438, 610, 1032, 1346
SduI GDGCHC 1 cut(s) 466
SfaNI GCATC 4 cut(s) 166, 227, 490, 1233
SfcI CTRYAG 3 cut(s) 37, 765, 1193
SmlI CTYRAG 2 cut(s) 431, 1301
SmoI CTYRAG 2 cut(s) 431, 1301
Sse9I AATT 4 cut(s) 261, 634, 787, 1037
SsiI CCGC 2 cut(s) 1138, 1388
SspMI CTAG 3 cut(s) 84, 684, 747
StyD4I CCNGG 4 cut(s) 436, 608, 1030, 1344
StyI CCWWGG 1 cut(s) 1130
TaaI ACNGT 4 cut(s) 691, 950, 980, 1176
TaiI ACGT 1 cut(s) 310
TaqI TCGA 4 cut(s) 291, 501, 891, 975
TasI AATT 4 cut(s) 261, 634, 787, 1037
TatI WGTACW 3 cut(s) 691, 945, 980
TfiI GAWTC 4 cut(s) 503, 546, 1063, 1367
Tru1I TTAA 6 cut(s) 296, 363, 522, 824, 1362, 1379
Tru9I TTAA 6 cut(s) 296, 363, 522, 824, 1362, 1379
TscAI CASTG 4 cut(s) 85, 694, 874, 1028
TseFI GTSAC 1 cut(s) 836
TseI GCWGC 4 cut(s) 119, 196, 337, 760
Tsp45I GTSAC 1 cut(s) 836
TspDTI ATGAA 3 cut(s) 15, 743, 1398
TspGWI ACGGA 3 cut(s) 509, 748, 819
TspRI CASTG 4 cut(s) 85, 694, 874, 1028
Van91I CCANNNNNTGG 1 cut(s) 650
VneI GTGCAC 1 cut(s) 462
XapI RAATTY 1 cut(s) 787
XceI RCATGY 1 cut(s) 643
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XspI CTAG 3 cut(s) 84, 684, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.