Rmu_co8411819.1_g000001

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8411819.1
OG0001519
Physical Location & Seq
Forward (+)
52 .. 1252
1201 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8411819.1_g000001.1.cds

Sequence Viewer

Length: 711 bp
atgtttcagaataaaactggacaaaatgtggtgtttagtagggcaagcaatatagaggcgtccaaattgaaggacaactgcattggcttctcttcgttattgtgtaattctgctaggaagacacagattttgcatacagacaatgctggagtaggacagaaacgacgccatagtgtggtagttgcagccagtcctcccacagaagatgctgtaattgctacggaaccgctgacgaaagaggatcttgtagcgtatcttgcctctggatgcaagcctaaggaagcatggagaattggcactgaacacgagaagtttggttttgagctcaaatctttacgtccagtgaaatatgaacaaatatctgagttgcttaatgggattgccgagagatttgattgggaaaaagttatggaaggtgactacattattggactcaaacagggtaagcaaagtatatcacttgagcctggtggccaatttgagcttagtggcgcacctcttgaaaccctgcatcaaacttgtgctgaagtcaattcacacctctatcaggtcaaagcagttacagaggaaatgggaattggatttcttggcattggttttcagcctaaatggggactgaaggacatacctataatgcccaaggtatttgttaatcctctttcaaccatattgagatgcagaaatgcatcatttggtgagataaggaattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

236

Amino Acids

26.11

Weight (kDa)

7.64

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 175
AciI CCGC 1 cut(s) 227
AclWI GGATC 1 cut(s) 249
AcoI YGGCCR 1 cut(s) 472
AcuI CTGAAG 2 cut(s) 546, 638
AcyI GRCGYC 2 cut(s) 59, 166
AfiI CCNNNNNNNGG 3 cut(s) 175, 547, 611
AgsI TTSAA 3 cut(s) 70, 503, 663
AjnI CCWGG 1 cut(s) 466
AjuI GAANNNNNNNTTGG 2 cut(s) 561, 593
AluBI AGCT 2 cut(s) 325, 484
AluI AGCT 2 cut(s) 325, 484
Alw21I GWGCWC 1 cut(s) 327
AlwI GGATC 1 cut(s) 249
AoxI GGCC 1 cut(s) 472
ApeKI GCWGC 1 cut(s) 185
ArsI GACNNNNNNTTYG 2 cut(s) 112, 144
AspLEI GCGC 1 cut(s) 494
AsuHPI GGTGA 2 cut(s) 428, 707
AxyI CCTNAGG 1 cut(s) 276
BalI TGGCCA 1 cut(s) 474
BanII GRGCYC 1 cut(s) 327
BauI CACGAG 1 cut(s) 305
BbsI GAAGAC 1 cut(s) 125
Bbv12I GWGCWC 1 cut(s) 327
BbvI GCAGC 1 cut(s) 197
BciT130I CCWGG 1 cut(s) 468
BfaI CTAG 1 cut(s) 114
BisI GCNGC 1 cut(s) 186
BlsI GCNGC 1 cut(s) 187
Bme1390I CCNGG 1 cut(s) 468
BmiI GGNNCC 1 cut(s) 225
BmrFI CCNGG 1 cut(s) 468
BmsI GCATC 5 cut(s) 196, 257, 520, 665, 695
BpiI GAAGAC 1 cut(s) 125
BpmI CTGGAG 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 482
BsaHI GRCGYC 2 cut(s) 59, 166
BsaJI CCNNGG 1 cut(s) 639
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 547, 611
Bse1I ACTGG 3 cut(s) 22, 189, 341
Bse21I CCTNAGG 1 cut(s) 276
BseBI CCWGG 1 cut(s) 468
BseDI CCNNGG 1 cut(s) 639
BseGI GGATG 1 cut(s) 272
BseLI CCNNNNNNNGG 3 cut(s) 175, 547, 611
BseMII CTCAG 1 cut(s) 354
BseNI ACTGG 3 cut(s) 22, 189, 341
BseXI GCAGC 1 cut(s) 197
BshFI GGCC 1 cut(s) 474
BsiHKAI GWGCWC 1 cut(s) 327
BslFI GGGAC 1 cut(s) 627
BslI CCNNNNNNNGG 3 cut(s) 175, 547, 611
BsmFI GGGAC 1 cut(s) 627
BsnI GGCC 1 cut(s) 474
Bsp1286I GDGCHC 1 cut(s) 327
Bsp143I GATC 1 cut(s) 241
BspACI CCGC 1 cut(s) 227
BspANI GGCC 1 cut(s) 474
BspCNI CTCAG 1 cut(s) 355
BspLI GGNNCC 1 cut(s) 225
BspPI GGATC 1 cut(s) 249
BsrI ACTGG 3 cut(s) 22, 189, 341
BssECI CCNNGG 1 cut(s) 639
BssMI GATC 1 cut(s) 241
BssNI GRCGYC 2 cut(s) 59, 166
BssSI CACGAG 1 cut(s) 305
BssT1I CCWWGG 1 cut(s) 639
Bst2BI CACGAG 1 cut(s) 305
Bst2UI CCWGG 1 cut(s) 468
Bst6I CTCTTC 1 cut(s) 97
BstACI GRCGYC 2 cut(s) 59, 166
BstC8I GCNNGC 2 cut(s) 46, 272
BstDEI CTNAG 3 cut(s) 276, 363, 485
BstENI CCTNNNNNAGG 1 cut(s) 545
BstF5I GGATG 1 cut(s) 272
BstHHI GCGC 1 cut(s) 494
BstKTI GATC 1 cut(s) 244
BstMBI GATC 1 cut(s) 241
BstMWI GCNNNNNNNGC 2 cut(s) 215, 257
BstNI CCWGG 1 cut(s) 468
BstSCI CCNGG 1 cut(s) 466
BstV1I GCAGC 1 cut(s) 197
BstV2I GAAGAC 1 cut(s) 125
BstX2I RGATCY 1 cut(s) 241
BstYI RGATCY 1 cut(s) 241
Bsu36I CCTNAGG 1 cut(s) 276
BsuRI GGCC 1 cut(s) 474
BtsCI GGATG 1 cut(s) 272
BtsIMutI CAGTG 2 cut(s) 297, 348
Cac8I GCNNGC 2 cut(s) 46, 272
CfoI GCGC 1 cut(s) 494
CseI GACGC 2 cut(s) 48, 174
CviAII CATG 1 cut(s) 285
CviJI RGCY 8 cut(s) 87, 188, 274, 325, 466, 474, 484, 604
CviKI_1 RGCY 8 cut(s) 87, 188, 274, 325, 466, 474, 484, 604
DdeI CTNAG 3 cut(s) 276, 363, 485
DpnI GATC 1 cut(s) 243
DpnII GATC 1 cut(s) 241
EaeI YGGCCR 1 cut(s) 472
Eam1104I CTCTTC 1 cut(s) 97
EarI CTCTTC 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 325
Eco130I CCWWGG 1 cut(s) 639
Eco24I GRGCYC 1 cut(s) 327
Eco53kI GAGCTC 1 cut(s) 325
Eco57I CTGAAG 2 cut(s) 546, 638
Eco81I CCTNAGG 1 cut(s) 276
EcoICRI GAGCTC 1 cut(s) 325
EcoNI CCTNNNNNAGG 1 cut(s) 545
EcoRII CCWGG 1 cut(s) 466
EcoT14I CCWWGG 1 cut(s) 639
EcoT22I ATGCAT 1 cut(s) 688
EcoT38I GRGCYC 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 639
FaeI CATG 1 cut(s) 288
FalI AAGNNNNNCTT 2 cut(s) 228, 260
FaqI GGGAC 1 cut(s) 627
FatI CATG 1 cut(s) 284
Fnu4HI GCNGC 1 cut(s) 186
FokI GGATG 1 cut(s) 279
FriOI GRGCYC 1 cut(s) 327
Fsp4HI GCNGC 1 cut(s) 186
FspBI CTAG 1 cut(s) 114
GlaI GCGC 1 cut(s) 493
GluI GCNGC 1 cut(s) 186
GsuI CTGGAG 1 cut(s) 168
HaeIII GGCC 1 cut(s) 474
HgaI GACGC 2 cut(s) 48, 174
HhaI GCGC 1 cut(s) 494
Hin1I GRCGYC 2 cut(s) 59, 166
Hin1II CATG 1 cut(s) 288
Hin6I GCGC 1 cut(s) 492
HinP1I GCGC 1 cut(s) 492
HinfI GANTC 1 cut(s) 432
HphI GGTGA 2 cut(s) 428, 707
Hpy188I TCNGA 2 cut(s) 9, 364
Hpy188III TCNNGA 2 cut(s) 264, 500
Hpy99I CGWCG 1 cut(s) 168
HpyAV CCTTC 3 cut(s) 64, 407, 613
HpyCH4IV ACGT 1 cut(s) 337
HpyCH4V TGCA 7 cut(s) 81, 133, 185, 270, 511, 678, 686
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 257
HpyF3I CTNAG 3 cut(s) 276, 363, 485
HpySE526I ACGT 1 cut(s) 337
Hsp92I GRCGYC 2 cut(s) 59, 166
Hsp92II CATG 1 cut(s) 288
HspAI GCGC 1 cut(s) 492
Kzo9I GATC 1 cut(s) 241
Lsp1109I GCAGC 1 cut(s) 197
LweI GCATC 5 cut(s) 196, 257, 520, 665, 695
MaeI CTAG 1 cut(s) 114
MaeII ACGT 1 cut(s) 337
MaeIII GTNAC 2 cut(s) 416, 559
MalI GATC 1 cut(s) 243
MboI GATC 1 cut(s) 241
MboII GAAGA 3 cut(s) 84, 130, 215
MflI RGATCY 1 cut(s) 241
MhlI GDGCHC 1 cut(s) 327
MlsI TGGCCA 1 cut(s) 474
MluCI AATT 8 cut(s) 65, 106, 213, 291, 476, 532, 576, 706
MluNI TGGCCA 1 cut(s) 474
MlyI GAGTC 1 cut(s) 426
MnlI CCTC 8 cut(s) 49, 204, 232, 271, 507, 551, 559, 666
Mox20I TGGCCA 1 cut(s) 474
Mph1103I ATGCAT 1 cut(s) 688
MscI TGGCCA 1 cut(s) 474
MseI TTAA 2 cut(s) 372, 651
Msp20I TGGCCA 1 cut(s) 474
MspA1I CMGCKG 1 cut(s) 229
MspR9I CCNGG 1 cut(s) 468
MvaI CCWGG 1 cut(s) 468
MwoI GCNNNNNNNGC 2 cut(s) 215, 257
NdeII GATC 1 cut(s) 241
NlaIII CATG 1 cut(s) 288
NlaIV GGNNCC 1 cut(s) 225
NmeAIII GCCGAG 1 cut(s) 409
NmuCI GTSAC 1 cut(s) 416
NsiI ATGCAT 1 cut(s) 688
PflMI CCANNNNNTGG 1 cut(s) 175
PkrI GCNGC 1 cut(s) 187
PleI GAGTC 1 cut(s) 426
PpsI GAGTC 1 cut(s) 426
Psp124BI GAGCTC 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 466
PspGI CCWGG 1 cut(s) 466
PspN4I GGNNCC 1 cut(s) 225
PsuI RGATCY 1 cut(s) 241
SacI GAGCTC 1 cut(s) 327
SaqAI TTAA 2 cut(s) 372, 651
SatI GCNGC 1 cut(s) 186
Sau3AI GATC 1 cut(s) 241
SchI GAGTC 1 cut(s) 426
ScrFI CCNGG 1 cut(s) 468
SduI GDGCHC 1 cut(s) 327
SetI ASST 9 cut(s) 327, 340, 418, 486, 499, 543, 552, 631, 645
SfaNI GCATC 5 cut(s) 196, 257, 520, 665, 695
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
Sse9I AATT 8 cut(s) 65, 106, 213, 291, 476, 532, 576, 706
SsiI CCGC 1 cut(s) 227
SspMI CTAG 1 cut(s) 114
SstI GAGCTC 1 cut(s) 327
StyD4I CCNGG 1 cut(s) 466
StyI CCWWGG 1 cut(s) 639
TaiI ACGT 1 cut(s) 340
TasI AATT 8 cut(s) 65, 106, 213, 291, 476, 532, 576, 706
Tru1I TTAA 2 cut(s) 372, 651
Tru9I TTAA 2 cut(s) 372, 651
TscAI CASTG 2 cut(s) 304, 348
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 1 cut(s) 185
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 1 cut(s) 366
TspGWI ACGGA 1 cut(s) 236
TspRI CASTG 2 cut(s) 304, 348
Van91I CCANNNNNTGG 1 cut(s) 175
XagI CCTNNNNNAGG 1 cut(s) 545
XspI CTAG 1 cut(s) 114
Zsp2I ATGCAT 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.