Rh7BG169800

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001519
Physical Location & Seq
Forward (+)
13207966 .. 13210084
2119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG169800.1

Sequence Viewer

Length: 918 bp
ATGGCACTCATTTCCCAGACAAGTTCATCATACTACTGCAGAAGCATTGGCTTCTCTTCCTCTGCATTTTATTCTACCACTGCTAGGAACATACAGATTTTGCATACAGACAATGTTGCAGCACAGAAACAAGGACGAAAGAGTGCGATAGTTGCTACAAGTCCTATCACAGAAGATGCAGTAGTACCTACAATGCCGCTGACAAAAGAAGATCTTGTAGGCCATCTTGCCTCTGGATGCAAGCCTAAAGAGAAATGGAGAATTGGCGTGGAACATGAGAGGTTTGGCTTCGATTTAAAAAGTTTACGTCCCATGCAATATCAACAAATAGCTCAGCTGCTAAATGGGATTGCTGAGAGATTTAAGTGGGAGAAAATCATGGAAGGTGATAACATTATTGGACTCAAACAGGGTAAGCAGAGTATATCACTTGAGCCTGGAGGTCAGTTTGAGCTGAGTGGTGCACCTCTTGAAACTCTGCATCAAGTTTGTACGGAAGTCGATTCACTACTCTGTCAGGGAAGATATGAGATTATTTATAATTACATGCCAAAAGTTGGCTCGCTTGGACATGATTTGATGTTCAGGACTAGCACTGTACAGGTCAATCTGGACTTCAGTTCTGAAGCAGACATGAAACGGAAACTTCGTGCTAGTCTTGCTTTGCAGCCTATAGCAACAGCCCTTCACATCTATACTGATACTGACAAGGATCGCACTGGCATTCTTCCTTTTGCTTTCGATGACTCCTTTGGGTTTGAGCGATATGTTGATTATGCTCTTGATGTTCCCATGTACTGTGTTTATCGGAAAAAGAAGTGTATCGACTGTACTGGAATGACCTTTCGGACATATTCATGTGATGGTGTTATCCATGAAGGACTTTATGGCAGGAAAGCTTCACTGCCTTCCGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

305

Amino Acids

34.12

Weight (kDa)

7.57

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 87 - 167 1.8e-07 Glutamate-cysteine ligase family 2(GCS2)
GCS2 PF04107 170 - 229 4.1e-12 Glutamate-cysteine ligase family 2(GCS2)
GCS2 PF04107 230 - 272 7.2e-10 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 540
AccB7I CCANNNNNTGG 1 cut(s) 557
AciI CCGC 1 cut(s) 197
AclWI GGATC 1 cut(s) 720
AcuI CTGAAG 2 cut(s) 601, 645
AfaI GTAC 5 cut(s) 186, 493, 600, 797, 832
AfiI CCNNNNNNNGG 2 cut(s) 84, 557
AgsI TTSAA 1 cut(s) 473
AjnI CCWGG 1 cut(s) 436
AluBI AGCT 4 cut(s) 332, 337, 454, 899
AluI AGCT 4 cut(s) 332, 337, 454, 899
Alw21I GWGCWC 1 cut(s) 466
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 1 cut(s) 720
AoxI GGCC 1 cut(s) 220
ApaLI GTGCAC 1 cut(s) 462
ApeKI GCWGC 3 cut(s) 119, 337, 667
AsuC2I CCSGG 1 cut(s) 913
AsuHPI GGTGA 1 cut(s) 398
BaeGI GKGCMC 1 cut(s) 466
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 3 cut(s) 131, 324, 679
BccI CCATC 2 cut(s) 231, 857
BciT130I CCWGG 1 cut(s) 438
BcnI CCSGG 1 cut(s) 913
BfaI CTAG 3 cut(s) 84, 591, 654
BfmI CTRYAG 2 cut(s) 37, 672
BglII AGATCT 1 cut(s) 211
BisI GCNGC 4 cut(s) 120, 197, 338, 668
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 4 cut(s) 121, 198, 339, 669
Bme1390I CCNGG 2 cut(s) 438, 913
BmrFI CCNGG 2 cut(s) 438, 913
BmsI GCATC 3 cut(s) 166, 227, 490
BpmI CTGGAG 1 cut(s) 459
Bpu1102I GCTNAGC 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 452
BpuMI CCSGG 1 cut(s) 913
Bsc4I CCNNNNNNNGG 2 cut(s) 84, 557
Bse1I ACTGG 2 cut(s) 724, 838
BseBI CCWGG 1 cut(s) 438
BseGI GGATG 1 cut(s) 242
BseLI CCNNNNNNNGG 2 cut(s) 84, 557
BseMII CTCAG 3 cut(s) 345, 347, 446
BseNI ACTGG 2 cut(s) 724, 838
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 3 cut(s) 131, 324, 679
BshFI GGCC 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 466
BsiSI CCGG 1 cut(s) 912
BslFI GGGAC 1 cut(s) 294
BslI CCNNNNNNNGG 2 cut(s) 84, 557
BsmFI GGGAC 1 cut(s) 294
BsmI GAATGC 1 cut(s) 723
BsnI GGCC 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 466
Bsp1407I TGTACA 1 cut(s) 598
Bsp143I GATC 2 cut(s) 211, 712
Bsp1720I GCTNAGC 1 cut(s) 333
BspACI CCGC 1 cut(s) 197
BspANI GGCC 1 cut(s) 222
BspCNI CTCAG 3 cut(s) 346, 346, 447
BspMAI CTGCAG 1 cut(s) 41
BspPI GGATC 1 cut(s) 720
BsrGI TGTACA 1 cut(s) 598
BsrI ACTGG 2 cut(s) 724, 838
BssMI GATC 2 cut(s) 211, 712
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 3 cut(s) 598, 800, 830
Bst6I CTCTTC 1 cut(s) 61
BstAUI TGTACA 1 cut(s) 598
BstC8I GCNNGC 2 cut(s) 242, 563
BstDEI CTNAG 3 cut(s) 333, 354, 455
BstF5I GGATG 1 cut(s) 242
BstKTI GATC 2 cut(s) 214, 715
BstMBI GATC 2 cut(s) 211, 712
BstMWI GCNNNNNNNGC 2 cut(s) 152, 659
BstNI CCWGG 1 cut(s) 438
BstNSI RCATGY 1 cut(s) 550
BstSCI CCNGG 2 cut(s) 436, 911
BstSFI CTRYAG 2 cut(s) 37, 672
BstSLI GKGCMC 1 cut(s) 466
BstV1I GCAGC 3 cut(s) 131, 324, 679
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BsuRI GGCC 1 cut(s) 222
BtsCI GGATG 1 cut(s) 242
BtsI GCAGTG 2 cut(s) 78, 902
BtsIMutI CAGTG 4 cut(s) 78, 594, 717, 902
Cac8I GCNNGC 2 cut(s) 242, 563
Csp6I GTAC 5 cut(s) 185, 492, 599, 796, 831
CviAII CATG 9 cut(s) 275, 313, 379, 547, 572, 634, 793, 858, 875
CviQI GTAC 5 cut(s) 185, 492, 599, 796, 831
DdeI CTNAG 3 cut(s) 333, 354, 455
DpnI GATC 2 cut(s) 213, 714
DpnII GATC 2 cut(s) 211, 712
DraI TTTAAA 1 cut(s) 297
Eam1104I CTCTTC 1 cut(s) 61
EarI CTCTTC 1 cut(s) 61
Eco57I CTGAAG 2 cut(s) 601, 645
EcoRII CCWGG 1 cut(s) 436
FaeI CATG 9 cut(s) 278, 316, 382, 550, 575, 637, 796, 861, 878
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FaqI GGGAC 1 cut(s) 294
FatI CATG 9 cut(s) 274, 312, 378, 546, 571, 633, 792, 857, 874
Fnu4HI GCNGC 4 cut(s) 120, 197, 338, 668
FokI GGATG 1 cut(s) 249
Fsp4HI GCNGC 4 cut(s) 120, 197, 338, 668
FspBI CTAG 3 cut(s) 84, 591, 654
GluI GCNGC 4 cut(s) 120, 197, 338, 668
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 1 cut(s) 222
HapII CCGG 1 cut(s) 912
Hin1II CATG 9 cut(s) 278, 316, 382, 550, 575, 637, 796, 861, 878
HindIII AAGCTT 1 cut(s) 897
HinfI GANTC 3 cut(s) 402, 503, 746
HpaII CCGG 1 cut(s) 912
HphI GGTGA 1 cut(s) 398
Hpy166II GTNNAC 2 cut(s) 305, 464
Hpy188I TCNGA 3 cut(s) 625, 810, 849
Hpy188III TCNNGA 5 cut(s) 234, 470, 586, 611, 782
Hpy8I GTNNAC 2 cut(s) 305, 464
HpyAV CCTTC 4 cut(s) 377, 695, 872, 918
HpyCH4III ACNGT 3 cut(s) 598, 800, 830
HpyCH4IV ACGT 1 cut(s) 307
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 659
HpyF3I CTNAG 3 cut(s) 333, 354, 455
HpySE526I ACGT 1 cut(s) 307
Hsp92II CATG 9 cut(s) 278, 316, 382, 550, 575, 637, 796, 861, 878
Kzo9I GATC 2 cut(s) 211, 712
Lsp1109I GCAGC 3 cut(s) 131, 324, 679
LweI GCATC 3 cut(s) 166, 227, 490
MaeI CTAG 3 cut(s) 84, 591, 654
MaeII ACGT 1 cut(s) 307
MalI GATC 2 cut(s) 213, 714
MboI GATC 2 cut(s) 211, 712
MboII GAAGA 5 cut(s) 48, 185, 221, 534, 719
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 1 cut(s) 466
MluCI AATT 2 cut(s) 261, 541
MlyI GAGTC 2 cut(s) 396, 740
MnlI CCTC 5 cut(s) 70, 241, 273, 434, 477
MseI TTAA 2 cut(s) 296, 363
MslI CAYNNNNRTG 1 cut(s) 856
MspA1I CMGCKG 2 cut(s) 199, 337
MspI CCGG 1 cut(s) 912
MspR9I CCNGG 2 cut(s) 438, 913
Mva1269I GAATGC 1 cut(s) 723
MvaI CCWGG 1 cut(s) 438
MwoI GCNNNNNNNGC 2 cut(s) 152, 659
NciI CCSGG 1 cut(s) 913
NdeII GATC 2 cut(s) 211, 712
NlaIII CATG 9 cut(s) 278, 316, 382, 550, 575, 637, 796, 861, 878
NspI RCATGY 1 cut(s) 550
PcsI WCGNNNNNNNCGW 1 cut(s) 646
PctI GAATGC 1 cut(s) 723
PfeI GAWTC 1 cut(s) 503
PflMI CCANNNNNTGG 1 cut(s) 557
PkrI GCNGC 4 cut(s) 121, 198, 339, 669
PleI GAGTC 2 cut(s) 396, 740
PpsI GAGTC 2 cut(s) 396, 740
PsiI TTATAA 1 cut(s) 540
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PstI CTGCAG 1 cut(s) 41
PsuI RGATCY 1 cut(s) 211
PvuII CAGCTG 1 cut(s) 337
RsaI GTAC 5 cut(s) 186, 493, 600, 797, 832
RsaNI GTAC 5 cut(s) 185, 492, 599, 796, 831
RseI CAYNNNNRTG 1 cut(s) 856
SaqAI TTAA 2 cut(s) 296, 363
SatI GCNGC 4 cut(s) 120, 197, 338, 668
Sau3AI GATC 2 cut(s) 211, 712
SchI GAGTC 2 cut(s) 396, 740
ScrFI CCNGG 2 cut(s) 438, 913
SduI GDGCHC 1 cut(s) 466
SfaNI GCATC 3 cut(s) 166, 227, 490
SfcI CTRYAG 2 cut(s) 37, 672
SmiMI CAYNNNNRTG 1 cut(s) 856
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
Sse9I AATT 2 cut(s) 261, 541
SsiI CCGC 1 cut(s) 197
SspMI CTAG 3 cut(s) 84, 591, 654
StyD4I CCNGG 2 cut(s) 436, 911
TaaI ACNGT 3 cut(s) 598, 800, 830
TaiI ACGT 1 cut(s) 310
TaqI TCGA 4 cut(s) 291, 501, 741, 825
TasI AATT 2 cut(s) 261, 541
TatI WGTACW 3 cut(s) 598, 795, 830
TauI GCSGC 1 cut(s) 199
TfiI GAWTC 1 cut(s) 503
Tru1I TTAA 2 cut(s) 296, 363
Tru9I TTAA 2 cut(s) 296, 363
TscAI CASTG 4 cut(s) 85, 601, 724, 909
TseI GCWGC 3 cut(s) 119, 337, 667
TspDTI ATGAA 4 cut(s) 15, 650, 846, 891
TspGWI ACGGA 2 cut(s) 509, 655
TspRI CASTG 4 cut(s) 85, 601, 724, 909
Van91I CCANNNNNTGG 1 cut(s) 557
VneI GTGCAC 1 cut(s) 462
XceI RCATGY 1 cut(s) 550
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XspI CTAG 3 cut(s) 84, 591, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.