Rh7CG176600

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0001519
Physical Location & Seq
Forward (+)
15075803 .. 15076562
760 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG176600.1

Sequence Viewer

Length: 384 bp
ATGAGGGGGGCTGATGGAGGGCCTTGGAGGAGGTTATGTGCTTTGCCTGCATTTTGGGTGGGTTTACTGTATCATGAGGTTTCCCTGGAGAATGTGTTAGACATGATAGCTGATTGGACTCCTGAAGAAAGACAGATGCTGAGAGATAAGGTTCCGAAAACGGGTCTAAAGACACCATTTCGTGATGGATTACTCAAGCAAGTTGCCGAAGATGTAGTAAAGTTTGCAAAGGATGGCCTGGAAAGAAGAGGCTTTAAGGAATCAGGGTTCTTAAATGAAGCGGTGGAGATTGTGAGAATACGTAATGACATGGCCTTTCAAATTTTTTCTTTTCCGGGGATATTGTCTAGTCCATATATGCATGGCAGGTGTAACACCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

127

Amino Acids

14.49

Weight (kDa)

7.8

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 1 - 97 5.4e-12 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 357
Acc36I ACCTGC 1 cut(s) 357
AciI CCGC 1 cut(s) 281
AcsI RAATTY 1 cut(s) 321
AcuI CTGAAG 1 cut(s) 144
AfiI CCNNNNNNNGG 1 cut(s) 161
AgsI TTSAA 1 cut(s) 320
AjnI CCWGG 2 cut(s) 84, 237
AloI GAACNNNNNNTCC 2 cut(s) 251, 283
AluBI AGCT 2 cut(s) 110, 381
AluI AGCT 2 cut(s) 110, 381
AlwNI CAGNNNCTG 1 cut(s) 139
AoxI GGCC 3 cut(s) 20, 235, 312
ApoI RAATTY 1 cut(s) 321
AspS9I GGNCC 1 cut(s) 20
AsuC2I CCSGG 1 cut(s) 336
BccI CCATC 3 cut(s) 8, 179, 227
BciT130I CCWGG 2 cut(s) 86, 239
BcnI CCSGG 1 cut(s) 336
BfaI CTAG 1 cut(s) 348
BfuAI ACCTGC 1 cut(s) 357
Bme1390I CCNGG 3 cut(s) 86, 239, 336
BmgT120I GGNCC 1 cut(s) 20
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 3 cut(s) 86, 239, 336
BmsI GCATC 1 cut(s) 126
BpmI CTGGAG 1 cut(s) 107
BpuEI CTTGAG 1 cut(s) 179
BpuMI CCSGG 1 cut(s) 336
BsaAI YACGTR 1 cut(s) 302
BsaJI CCNNGG 3 cut(s) 23, 84, 335
BsaXI ACNNNNNCTCC 4 cut(s) 22, 52, 80, 110
Bsc4I CCNNNNNNNGG 1 cut(s) 161
BseBI CCWGG 2 cut(s) 86, 239
BseDI CCNNGG 3 cut(s) 23, 84, 335
BseGI GGATG 1 cut(s) 238
BseLI CCNNNNNNNGG 1 cut(s) 161
BseMII CTCAG 1 cut(s) 131
BseRI GAGGAG 1 cut(s) 43
BshFI GGCC 3 cut(s) 22, 237, 314
BsiSI CCGG 1 cut(s) 335
BslI CCNNNNNNNGG 1 cut(s) 161
BsnI GGCC 3 cut(s) 22, 237, 314
BspACI CCGC 1 cut(s) 281
BspANI GGCC 3 cut(s) 22, 237, 314
BspCNI CTCAG 1 cut(s) 132
BspHI TCATGA 1 cut(s) 73
BspLI GGNNCC 1 cut(s) 153
BspMI ACCTGC 1 cut(s) 357
BssECI CCNNGG 3 cut(s) 23, 84, 335
BssT1I CCWWGG 1 cut(s) 23
Bst2UI CCWGG 2 cut(s) 86, 239
Bst4CI ACNGT 1 cut(s) 69
Bst6I CTCTTC 1 cut(s) 241
BstBAI YACGTR 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 48
BstDEI CTNAG 1 cut(s) 140
BstF5I GGATG 1 cut(s) 238
BstMWI GCNNNNNNNGC 1 cut(s) 47
BstNI CCWGG 2 cut(s) 86, 239
BstSCI CCNGG 3 cut(s) 84, 237, 334
BstSNI TACGTA 1 cut(s) 302
BsuRI GGCC 3 cut(s) 22, 237, 314
BtsCI GGATG 1 cut(s) 238
BveI ACCTGC 1 cut(s) 357
Cac8I GCNNGC 1 cut(s) 48
CaiI CAGNNNCTG 1 cut(s) 139
CciI TCATGA 1 cut(s) 73
Cfr13I GGNCC 1 cut(s) 20
CviAII CATG 4 cut(s) 74, 103, 310, 362
CviJI RGCY 7 cut(s) 11, 22, 110, 237, 252, 314, 381
CviKI_1 RGCY 7 cut(s) 11, 22, 110, 237, 252, 314, 381
DdeI CTNAG 1 cut(s) 140
Eam1104I CTCTTC 1 cut(s) 241
EarI CTCTTC 1 cut(s) 241
Eco105I TACGTA 1 cut(s) 302
Eco130I CCWWGG 1 cut(s) 23
Eco57I CTGAAG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 20
EcoRII CCWGG 2 cut(s) 84, 237
EcoT14I CCWWGG 1 cut(s) 23
EcoT22I ATGCAT 1 cut(s) 363
ErhI CCWWGG 1 cut(s) 23
FaeI CATG 4 cut(s) 77, 106, 313, 365
FaiI YATR 8 cut(s) 37, 75, 104, 311, 355, 357, 359, 363
FatI CATG 4 cut(s) 73, 102, 309, 361
FokI GGATG 1 cut(s) 245
FspBI CTAG 1 cut(s) 348
GsuI CTGGAG 1 cut(s) 107
HaeIII GGCC 3 cut(s) 22, 237, 314
HapII CCGG 1 cut(s) 335
Hin1II CATG 4 cut(s) 77, 106, 313, 365
HinfI GANTC 2 cut(s) 118, 260
HpaII CCGG 1 cut(s) 335
Hpy166II GTNNAC 1 cut(s) 65
Hpy188I TCNGA 1 cut(s) 156
Hpy188III TCNNGA 3 cut(s) 74, 122, 182
Hpy8I GTNNAC 1 cut(s) 65
HpyCH4III ACNGT 1 cut(s) 69
HpyCH4IV ACGT 1 cut(s) 301
HpyCH4V TGCA 3 cut(s) 50, 227, 361
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
HpyF3I CTNAG 1 cut(s) 140
HpySE526I ACGT 1 cut(s) 301
Hsp92II CATG 4 cut(s) 77, 106, 313, 365
LpnPI CCDG 9 cut(s) 60, 71, 98, 135, 224, 249, 251, 348, 352
LweI GCATC 1 cut(s) 126
MaeI CTAG 1 cut(s) 348
MaeII ACGT 1 cut(s) 301
MaeIII GTNAC 1 cut(s) 371
MboII GAAGA 3 cut(s) 137, 221, 258
MluCI AATT 1 cut(s) 321
MlyI GAGTC 1 cut(s) 112
MnlI CCTC 5 cut(s) 11, 21, 24, 70, 242
Mph1103I ATGCAT 1 cut(s) 363
MseI TTAA 2 cut(s) 255, 272
MspA1I CMGCKG 1 cut(s) 381
MspI CCGG 1 cut(s) 335
MspR9I CCNGG 3 cut(s) 86, 239, 336
MvaI CCWGG 2 cut(s) 86, 239
MwoI GCNNNNNNNGC 1 cut(s) 47
NciI CCSGG 1 cut(s) 336
NlaIII CATG 4 cut(s) 77, 106, 313, 365
NlaIV GGNNCC 1 cut(s) 153
NsiI ATGCAT 1 cut(s) 363
PagI TCATGA 1 cut(s) 73
PaqCI CACCTGC 1 cut(s) 357
PfeI GAWTC 1 cut(s) 260
PleI GAGTC 1 cut(s) 112
PpsI GAGTC 1 cut(s) 112
Ppu21I YACGTR 1 cut(s) 302
Psp6I CCWGG 2 cut(s) 84, 237
PspGI CCWGG 2 cut(s) 84, 237
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 1 cut(s) 20
PstNI CAGNNNCTG 1 cut(s) 139
PvuII CAGCTG 1 cut(s) 381
SaqAI TTAA 2 cut(s) 255, 272
Sau96I GGNCC 1 cut(s) 20
SchI GAGTC 1 cut(s) 112
ScrFI CCNGG 3 cut(s) 86, 239, 336
SetI ASST 7 cut(s) 35, 81, 112, 153, 304, 371, 383
SfaNI GCATC 1 cut(s) 126
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
SnaBI TACGTA 1 cut(s) 302
Sse9I AATT 1 cut(s) 321
SsiI CCGC 1 cut(s) 281
SspMI CTAG 1 cut(s) 348
StyD4I CCNGG 3 cut(s) 84, 237, 334
StyI CCWWGG 1 cut(s) 23
TaaI ACNGT 1 cut(s) 69
TaiI ACGT 1 cut(s) 304
TasI AATT 1 cut(s) 321
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 2 cut(s) 255, 272
Tru9I TTAA 2 cut(s) 255, 272
TspDTI ATGAA 1 cut(s) 291
XapI RAATTY 1 cut(s) 321
XspI CTAG 1 cut(s) 348
Zsp2I ATGCAT 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.