Rorug07G0043200

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001519
Physical Location & Seq
Forward (+)
3098604 .. 3134696
36093 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0043200.1

Sequence Viewer

Length: 1614 bp
ATGGAGGTTCTGGCATGGAACTGTCGTGGTCTTAATAACACCGCGTCGCTTCAGGCGCTAAAATTGTTGATCCAACAACAGAGACCCTCAATCATCTTTCTTTCGGAGACGAAGGTGGATGATTGGAACTATCTGAATAATCTGCGACTGCAGATTGGATATCTCAACTGTGAAGTAGTCTTCAGTGCAGGGCAGAGTGGAGGTGTGGCTCTGTTTTGGGGAGATGGACTGGATATCAGGTTCCGTTCGAAGAGTCTAAACCATATTGATGTTGAGGTGCATGAGAAGGACGGCTCCGGCATTGCCTGGCGGTTGACTGGGCTTTATGGTCACCCTTCTACTGCTGATCGTTACCGAACGTGGGATTTACTTTGTTCTTTAAGTGCTGAGTGTGCTCTACCTTGGGTGGTGGTGGGAGATATGAATGAGCTTCTTCATGCCTCTGAGAAAGAAGGAGGGGCAGTGCGCAGGGAAGGCCAAATGCAGCCATTCCGGGATTGCTTGTCAGAGTGTGATCTTTATGATTTAGGATTTCAAGGGCAGCCATATACATGGCGTGGTCCAGGCATGCGAAGCTGTCTAGATCGAGCTGTAGCCACTGCAACATGGTCGGATGTTTTTACCGCAGCCAAGGTTGTGCATTTGCCTCCAGTTCATGGTGACCACATTCCAATTCTGTTGGGTGTTCATCGCGGTAATCTCCCCGCTGTGGAACGTCGTCGATCCCGGTTTCGGTTTGAGAGTTTTTGGACTTTGCATGATGGTTGTCAGGATGTGGTGCGTCAAGGTTGGGAGATGACAGCGGTAGGTTTGCCTATGCTTCAAGTCAAGAAGAAGATAATGCAGACTCGCTTCTCTCTAAATCAGTGGCAGCGAGCCACGTTTGGGGTCAGGAGCCGTGAGATTGAGTACATAAGGGACAGATTGCAGGTCCTTTATTCACTGCCTATGACTCAGATACACCAGGAGGAATGCTTTGATCTGCACTCTAAGTTAGAAGGGCTTTTAACTGATGCGAGTGCCTATTGGAGGCAACGCTCTAAGGTGATTTGGCTTACAGAAGGGGATAGAAACACGAAGTATTTCCATAGGAAGGCTTCCAATCGTAGAGCTAAGAACAGATTACTTGGTTTGTTTGATCACAATGGGGTCTGGCAGGATTCCATGAAAGGAATGGAAGAGGTTGTCTTGCGTTATTTCACTGTTCTTGGTCTGAGGAGTCAGAGCTCCATTGAAGAAGTGAAAGAGGCCTACGACAACGTTTCTTCAACGTGGAATTCAGAAGCGCAAGCTCCGGATTCTCTTGAATTTATAAAGGATGTAATTGAGAAGCTCAAAAAGCAATATGCTGGAGAAAGCTTTTCATGTATAGACCTTCCTCTACTAGAGTCTGCTGCTTATGATCATAACCTTCCGGTCATTACCTCTAAGGATTTCCAGACCATGTTTCAGGACAACAAGCCCTGGCTAATTCAGTTGTGGTCATTTGGGAGCAAAAGCTGTGATCAATTTTCTCATGCCTGGAAAAGAATTGCTGGTTTCTTGGATGGTGTGGCAAATGCTGCTCAGGTAGATCTTGAAGTGATTCAGCTGGCAACGTATCTTGCTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

537

Amino Acids

61.46

Weight (kDa)

6.45

Isoelectric Point (pI)

47.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 5 - 207 1.2e-15 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1313
Acc16I TGCGCA 1 cut(s) 467
Acc36I ACCTGC 1 cut(s) 919
AccB7I CCANNNNNTGG 1 cut(s) 656
AccII CGCG 2 cut(s) 44, 693
AccIII TCCGGA 1 cut(s) 1294
AciI CCGC 6 cut(s) 42, 310, 624, 693, 705, 803
AclI AACGTT 1 cut(s) 1260
AclWI GGATC 2 cut(s) 64, 717
AcsI RAATTY 2 cut(s) 1276, 1307
AcuI CTGAAG 2 cut(s) 35, 166
AfaI GTAC 1 cut(s) 911
AfiI CCNNNNNNNGG 7 cut(s) 361, 656, 709, 732, 885, 1029, 1093
AgsI TTSAA 6 cut(s) 536, 824, 1235, 1269, 1307, 1580
AjnI CCWGG 5 cut(s) 305, 562, 963, 1463, 1520
AloI GAACNNNNNNTCC 2 cut(s) 224, 256
Alw21I GWGCWC 2 cut(s) 397, 1229
Alw26I GTCTC 2 cut(s) 76, 101
AlwI GGATC 2 cut(s) 64, 717
Aor13HI TCCGGA 1 cut(s) 1294
AoxI GGCC 2 cut(s) 475, 1248
ApeKI GCWGC 6 cut(s) 484, 541, 626, 871, 1394, 1562
ApoI RAATTY 2 cut(s) 1276, 1307
Asp700I GAANNNNTTC 2 cut(s) 1082, 1584
AspLEI GCGC 3 cut(s) 58, 468, 1288
AspS9I GGNCC 2 cut(s) 560, 931
AsuC2I CCSGG 2 cut(s) 494, 727
AsuHPI GGTGA 3 cut(s) 323, 671, 1057
AsuII TTCGAA 1 cut(s) 248
AvaII GGWCC 2 cut(s) 560, 931
BanII GRGCYC 1 cut(s) 1229
BbsI GAAGAC 1 cut(s) 172
Bbv12I GWGCWC 2 cut(s) 397, 1229
BbvI GCAGC 6 cut(s) 496, 553, 638, 883, 1381, 1549
BccI CCATC 3 cut(s) 218, 755, 1541
BceAI ACGGC 2 cut(s) 307, 882
BcgI CGANNNNNNTGC 2 cut(s) 591, 625
BciT130I CCWGG 5 cut(s) 307, 564, 965, 1465, 1522
BclI TGATCA 3 cut(s) 1138, 1402, 1504
BcnI CCSGG 2 cut(s) 494, 727
BcoDI GTCTC 2 cut(s) 76, 101
BfaI CTAG 2 cut(s) 581, 1385
BfmI CTRYAG 2 cut(s) 149, 591
BfoI RGCGCY 1 cut(s) 59
BfuAI ACCTGC 1 cut(s) 919
BglII AGATCT 1 cut(s) 1573
BisI GCNGC 6 cut(s) 485, 542, 627, 872, 1395, 1563
BlsI GCNGC 6 cut(s) 486, 543, 628, 873, 1396, 1564
Bme1390I CCNGG 7 cut(s) 307, 494, 564, 727, 965, 1465, 1522
Bme18I GGWCC 2 cut(s) 560, 931
BmgT120I GGNCC 2 cut(s) 560, 931
BmiI GGNNCC 3 cut(s) 242, 295, 896
BmrFI CCNGG 7 cut(s) 307, 494, 564, 727, 965, 1465, 1522
BmrI ACTGGG 1 cut(s) 327
BmsI GCATC 1 cut(s) 1003
BmuI ACTGGG 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 172
BpmI CTGGAG 2 cut(s) 633, 1371
Bpu10I CCTNAGC 1 cut(s) 1566
Bpu14I TTCGAA 1 cut(s) 248
BpuMI CCSGG 2 cut(s) 494, 727
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 3 cut(s) 401, 630, 1463
BsaWI WCCGGW 2 cut(s) 1294, 1414
BsaXI ACNNNNNCTCC 2 cut(s) 447, 477
Bsc4I CCNNNNNNNGG 7 cut(s) 361, 656, 709, 732, 885, 1029, 1093
Bse1I ACTGG 3 cut(s) 234, 322, 650
Bse3DI GCAATG 1 cut(s) 300
BseAI TCCGGA 1 cut(s) 1294
BseBI CCWGG 5 cut(s) 307, 564, 965, 1465, 1522
BseDI CCNNGG 3 cut(s) 401, 630, 1463
BseGI GGATG 5 cut(s) 124, 619, 778, 1324, 1552
BseLI CCNNNNNNNGG 7 cut(s) 361, 656, 709, 732, 885, 1029, 1093
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 5 cut(s) 378, 435, 968, 1205, 1580
BseNI ACTGG 3 cut(s) 234, 322, 650
BseRI GAGGAG 1 cut(s) 1231
BseXI GCAGC 6 cut(s) 496, 553, 638, 883, 1381, 1549
BsgI GTGCAG 2 cut(s) 207, 968
Bsh1236I CGCG 2 cut(s) 44, 693
BshFI GGCC 2 cut(s) 477, 1250
BsiHKAI GWGCWC 2 cut(s) 397, 1229
BsiSI CCGG 5 cut(s) 297, 493, 727, 1295, 1415
BslFI GGGAC 1 cut(s) 932
BslI CCNNNNNNNGG 7 cut(s) 361, 656, 709, 732, 885, 1029, 1093
BsmAI GTCTC 2 cut(s) 76, 101
BsmBI CGTCTC 1 cut(s) 101
BsmFI GGGAC 1 cut(s) 932
BsmI GAATGC 1 cut(s) 977
BsnI GGCC 2 cut(s) 477, 1250
Bso31I GGTCTC 1 cut(s) 76
Bsp119I TTCGAA 1 cut(s) 248
Bsp1286I GDGCHC 2 cut(s) 397, 1229
Bsp13I TCCGGA 1 cut(s) 1294
BspACI CCGC 6 cut(s) 42, 310, 624, 693, 705, 803
BspANI GGCC 2 cut(s) 477, 1250
BspCNI CTCAG 5 cut(s) 379, 436, 967, 1206, 1579
BspEI TCCGGA 1 cut(s) 1294
BspFNI CGCG 2 cut(s) 44, 693
BspLI GGNNCC 3 cut(s) 242, 295, 896
BspMAI CTGCAG 1 cut(s) 153
BspMI ACCTGC 1 cut(s) 919
BspPI GGATC 2 cut(s) 64, 717
BspT104I TTCGAA 1 cut(s) 248
BspTNI GGTCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 300
BsrI ACTGG 3 cut(s) 234, 322, 650
BssECI CCNNGG 3 cut(s) 401, 630, 1463
BssT1I CCWWGG 2 cut(s) 401, 630
Bst2UI CCWGG 5 cut(s) 307, 564, 965, 1465, 1522
Bst4CI ACNGT 3 cut(s) 23, 170, 1204
Bst6I CTCTTC 2 cut(s) 245, 1173
BstAPI GCANNNNNTGC 1 cut(s) 1562
BstBI TTCGAA 1 cut(s) 248
BstC8I GCNNGC 4 cut(s) 569, 876, 1290, 1593
BstDEI CTNAG 9 cut(s) 387, 444, 954, 990, 1041, 1113, 1214, 1428, 1566
BstEII GGTNACC 2 cut(s) 329, 659
BstENI CCTNNNNNAGG 1 cut(s) 1027
BstF5I GGATG 5 cut(s) 124, 619, 778, 1324, 1552
BstFNI CGCG 2 cut(s) 44, 693
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 3 cut(s) 58, 468, 1288
BstMAI GTCTC 2 cut(s) 76, 101
BstMWI GCNNNNNNNGC 6 cut(s) 55, 392, 474, 573, 1339, 1562
BstNI CCWGG 5 cut(s) 307, 564, 965, 1465, 1522
BstNSI RCATGY 1 cut(s) 571
BstPI GGTNACC 2 cut(s) 329, 659
BstSCI CCNGG 7 cut(s) 305, 492, 562, 725, 963, 1463, 1520
BstSFI CTRYAG 2 cut(s) 149, 591
BstUI CGCG 2 cut(s) 44, 693
BstV1I GCAGC 6 cut(s) 496, 553, 638, 883, 1381, 1549
BstV2I GAAGAC 1 cut(s) 172
BstX2I RGATCY 1 cut(s) 1573
BstXI CCANNNNNNTGG 1 cut(s) 552
BstYI RGATCY 1 cut(s) 1573
BsuRI GGCC 2 cut(s) 477, 1250
BtgZI GCGATG 1 cut(s) 674
BtsCI GGATG 5 cut(s) 124, 619, 778, 1324, 1552
BtsI GCAGTG 3 cut(s) 468, 597, 941
BtsIMutI CAGTG 6 cut(s) 190, 468, 597, 872, 941, 1200
BveI ACCTGC 1 cut(s) 919
Cac8I GCNNGC 4 cut(s) 569, 876, 1290, 1593
CfoI GCGC 3 cut(s) 58, 468, 1288
Cfr13I GGNCC 2 cut(s) 560, 931
CseI GACGC 2 cut(s) 33, 770
Csp6I GTAC 1 cut(s) 910
CviQI GTAC 1 cut(s) 910
DdeI CTNAG 9 cut(s) 387, 444, 954, 990, 1041, 1113, 1214, 1428, 1566
Eam1104I CTCTTC 2 cut(s) 245, 1173
EarI CTCTTC 2 cut(s) 245, 1173
Ecl136II GAGCTC 1 cut(s) 1227
Eco130I CCWWGG 2 cut(s) 401, 630
Eco147I AGGCCT 1 cut(s) 1250
Eco24I GRGCYC 1 cut(s) 1229
Eco31I GGTCTC 1 cut(s) 76
Eco32I GATATC 2 cut(s) 161, 235
Eco47I GGWCC 2 cut(s) 560, 931
Eco53kI GAGCTC 1 cut(s) 1227
Eco57I CTGAAG 2 cut(s) 35, 166
Eco91I GGTNACC 2 cut(s) 329, 659
EcoICRI GAGCTC 1 cut(s) 1227
EcoNI CCTNNNNNAGG 1 cut(s) 1027
EcoO109I RGGNCCY 1 cut(s) 931
EcoO65I GGTNACC 2 cut(s) 329, 659
EcoRI GAATTC 1 cut(s) 1276
EcoRII CCWGG 5 cut(s) 305, 562, 963, 1463, 1520
EcoRV GATATC 2 cut(s) 161, 235
EcoT14I CCWWGG 2 cut(s) 401, 630
EcoT38I GRGCYC 1 cut(s) 1229
ErhI CCWWGG 2 cut(s) 401, 630
Esp3I CGTCTC 1 cut(s) 101
FaqI GGGAC 1 cut(s) 932
FauI CCCGC 1 cut(s) 712
FbaI TGATCA 3 cut(s) 1138, 1402, 1504
Fnu4HI GCNGC 6 cut(s) 485, 542, 627, 872, 1395, 1563
FokI GGATG 5 cut(s) 131, 626, 785, 1331, 1559
FriOI GRGCYC 1 cut(s) 1229
Fsp4HI GCNGC 6 cut(s) 485, 542, 627, 872, 1395, 1563
FspBI CTAG 2 cut(s) 581, 1385
FspI TGCGCA 1 cut(s) 467
GlaI GCGC 3 cut(s) 57, 467, 1287
GluI GCNGC 6 cut(s) 485, 542, 627, 872, 1395, 1563
GsuI CTGGAG 2 cut(s) 633, 1371
HaeII RGCGCY 1 cut(s) 59
HaeIII GGCC 2 cut(s) 477, 1250
HapII CCGG 5 cut(s) 297, 493, 727, 1295, 1415
HgaI GACGC 2 cut(s) 33, 770
HhaI GCGC 3 cut(s) 58, 468, 1288
Hin6I GCGC 3 cut(s) 56, 466, 1286
HinP1I GCGC 3 cut(s) 56, 466, 1286
HincII GTYRAC 1 cut(s) 315
HindII GTYRAC 1 cut(s) 315
HindIII AAGCTT 1 cut(s) 1357
HinfI GANTC 8 cut(s) 253, 847, 952, 1160, 1219, 1298, 1388, 1585
HpaII CCGG 5 cut(s) 297, 493, 727, 1295, 1415
HphI GGTGA 3 cut(s) 323, 671, 1057
Hpy166II GTNNAC 1 cut(s) 315
Hpy188I TCNGA 9 cut(s) 106, 135, 445, 508, 613, 957, 1215, 1224, 1282
Hpy188III TCNNGA 9 cut(s) 581, 770, 829, 892, 1295, 1304, 1438, 1451, 1577
Hpy8I GTNNAC 1 cut(s) 315
Hpy99I CGWCG 3 cut(s) 49, 720, 723
HpyCH4III ACNGT 3 cut(s) 23, 170, 1204
HpyCH4IV ACGT 6 cut(s) 359, 715, 881, 1260, 1271, 1598
HpyF10VI GCNNNNNNNGC 6 cut(s) 55, 392, 474, 573, 1339, 1562
HpyF3I CTNAG 9 cut(s) 387, 444, 954, 990, 1041, 1113, 1214, 1428, 1566
HpySE526I ACGT 6 cut(s) 359, 715, 881, 1260, 1271, 1598
HspAI GCGC 3 cut(s) 56, 466, 1286
Kpn2I TCCGGA 1 cut(s) 1294
Ksp22I TGATCA 3 cut(s) 1138, 1402, 1504
LmnI GCTCC 5 cut(s) 299, 894, 1232, 1297, 1491
Lsp1109I GCAGC 6 cut(s) 496, 553, 638, 883, 1381, 1549
LweI GCATC 1 cut(s) 1003
MaeI CTAG 2 cut(s) 581, 1385
MaeII ACGT 6 cut(s) 359, 715, 881, 1260, 1271, 1598
MaeIII GTNAC 3 cut(s) 329, 350, 659
MboII GAAGA 8 cut(s) 172, 262, 425, 844, 847, 1190, 1247, 1257
MflI RGATCY 1 cut(s) 1573
MhlI GDGCHC 2 cut(s) 397, 1229
MluCI AATT 8 cut(s) 62, 672, 1276, 1307, 1323, 1470, 1508, 1530
MlyI GAGTC 5 cut(s) 262, 841, 946, 1228, 1397
MmeI TCCRAC 2 cut(s) 97, 591
MroI TCCGGA 1 cut(s) 1294
MroXI GAANNNNTTC 2 cut(s) 1082, 1584
MseI TTAA 3 cut(s) 33, 380, 1007
MslI CAYNNNNRTG 2 cut(s) 267, 550
MspA1I CMGCKG 3 cut(s) 707, 803, 1591
MspI CCGG 5 cut(s) 297, 493, 727, 1295, 1415
MspR9I CCNGG 7 cut(s) 307, 494, 564, 727, 965, 1465, 1522
Mva1269I GAATGC 1 cut(s) 977
MvaI CCWGG 5 cut(s) 307, 564, 965, 1465, 1522
MvnI CGCG 2 cut(s) 44, 693
MwoI GCNNNNNNNGC 6 cut(s) 55, 392, 474, 573, 1339, 1562
NciI CCSGG 2 cut(s) 494, 727
NlaIV GGNNCC 3 cut(s) 242, 295, 896
NmuCI GTSAC 2 cut(s) 329, 659
NsbI TGCGCA 1 cut(s) 467
NspI RCATGY 1 cut(s) 571
NspV TTCGAA 1 cut(s) 248
PaeI GCATGC 1 cut(s) 571
PceI AGGCCT 1 cut(s) 1250
PctI GAATGC 1 cut(s) 977
PdmI GAANNNNTTC 2 cut(s) 1082, 1584
PfeI GAWTC 3 cut(s) 1160, 1298, 1585
PflMI CCANNNNNTGG 1 cut(s) 656
PfoI TCCNGGA 1 cut(s) 492
PkrI GCNGC 6 cut(s) 486, 543, 628, 873, 1396, 1564
PleI GAGTC 5 cut(s) 261, 841, 946, 1227, 1396
PpsI GAGTC 5 cut(s) 261, 841, 946, 1227, 1396
PpuMI RGGWCCY 1 cut(s) 931
PsiI TTATAA 1 cut(s) 1313
Psp124BI GAGCTC 1 cut(s) 1229
Psp1406I AACGTT 1 cut(s) 1260
Psp5II RGGWCCY 1 cut(s) 931
Psp6I CCWGG 5 cut(s) 305, 562, 963, 1463, 1520
PspEI GGTNACC 2 cut(s) 329, 659
PspGI CCWGG 5 cut(s) 305, 562, 963, 1463, 1520
PspN4I GGNNCC 3 cut(s) 242, 295, 896
PspPI GGNCC 2 cut(s) 560, 931
PspPPI RGGWCCY 1 cut(s) 931
PstI CTGCAG 1 cut(s) 153
PsuI RGATCY 1 cut(s) 1573
PvuII CAGCTG 1 cut(s) 1591
RsaI GTAC 1 cut(s) 911
RsaNI GTAC 1 cut(s) 910
RseI CAYNNNNRTG 2 cut(s) 267, 550
SacI GAGCTC 1 cut(s) 1229
SaqAI TTAA 3 cut(s) 33, 380, 1007
SatI GCNGC 6 cut(s) 485, 542, 627, 872, 1395, 1563
Sau96I GGNCC 2 cut(s) 560, 931
SchI GAGTC 5 cut(s) 262, 841, 946, 1228, 1397
ScrFI CCNGG 7 cut(s) 307, 494, 564, 727, 965, 1465, 1522
SduI GDGCHC 2 cut(s) 397, 1229
SfaNI GCATC 1 cut(s) 1003
SfcI CTRYAG 2 cut(s) 149, 591
SfuI TTCGAA 1 cut(s) 248
SinI GGWCC 2 cut(s) 560, 931
SmiMI CAYNNNNRTG 2 cut(s) 267, 550
SphI GCATGC 1 cut(s) 571
Sse9I AATT 8 cut(s) 62, 672, 1276, 1307, 1323, 1470, 1508, 1530
SseBI AGGCCT 1 cut(s) 1250
SsiI CCGC 6 cut(s) 42, 310, 624, 693, 705, 803
SspMI CTAG 2 cut(s) 581, 1385
SstI GAGCTC 1 cut(s) 1229
StuI AGGCCT 1 cut(s) 1250
StyD4I CCNGG 7 cut(s) 305, 492, 562, 725, 963, 1463, 1520
StyI CCWWGG 2 cut(s) 401, 630
TaaI ACNGT 3 cut(s) 23, 170, 1204
TaiI ACGT 6 cut(s) 362, 718, 884, 1263, 1274, 1601
TaqI TCGA 3 cut(s) 248, 586, 721
TasI AATT 8 cut(s) 62, 672, 1276, 1307, 1323, 1470, 1508, 1530
TatI WGTACW 1 cut(s) 909
TfiI GAWTC 3 cut(s) 1160, 1298, 1585
Tru1I TTAA 3 cut(s) 33, 380, 1007
Tru9I TTAA 3 cut(s) 33, 380, 1007
TscAI CASTG 6 cut(s) 190, 468, 604, 872, 948, 1207
TseFI GTSAC 2 cut(s) 329, 659
TseI GCWGC 6 cut(s) 484, 541, 626, 871, 1394, 1562
Tsp45I GTSAC 2 cut(s) 329, 659
TspDTI ATGAA 6 cut(s) 425, 437, 644, 677, 1181, 1353
TspGWI ACGGA 1 cut(s) 233
TspRI CASTG 6 cut(s) 190, 468, 604, 872, 948, 1207
Van91I CCANNNNNTGG 1 cut(s) 656
VpaK11BI GGWCC 2 cut(s) 560, 931
XagI CCTNNNNNAGG 1 cut(s) 1027
XapI RAATTY 2 cut(s) 1276, 1307
XbaI TCTAGA 1 cut(s) 580
XceI RCATGY 1 cut(s) 571
XcmI CCANNNNNNNNNTGG 1 cut(s) 1171
XmnI GAANNNNTTC 2 cut(s) 1082, 1584
XspI CTAG 2 cut(s) 581, 1385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.