Rh1BG086200

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001519
Physical Location & Seq
Reverse (-)
13598695 .. 13603710
5016 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG086200.1

Sequence Viewer

Length: 1320 bp
ATGGCACTCACTTCCCAGACAAGTTTATCATACTACTGCAGAAGCATTGGCTTCTCTTCCTTAGCATTTTATTCTACTACTGCGAGGAAGATACAGATTTTGGATACAGTCAATGTTGCAGGACAGAAACAAGGACGTAAAAGTGCGATAGTTGCTTCAAGTCCTATCACAGAAGATGCTGTAATAGCTACAATGCCGCTGACGAAAGAAGATCTTGTAGGCCATCTTGCCTCTGGATGCAAGCCTAAAGAAAAATGGAGAATTGGCATGGAACATGAGAGGTTTGGCTTCAATTTAAAAAGTTTACGTCCCCTGCAATATCAACAAATAGCTGAGCTGCTAAATGGGATTGCTGAGAGATTTGAGTGGGAGAAAATCATGGAAGGTGATAACATTATTGGACTCAAACAGGGCAAGCAGAGTATATCACTTGAGCCTGGAGGTCAGTTTGAGCTTAGTGGTGCACCTCTTGAAACTCTGCATCAAGTTTGTATGGCAGTCGATTCACAACTCTATCAGGTTAAAGCAGTTGCAGAGAAAATGGGAATTGGATTTCTTGAAATTGGTGAGCAGCCGAAATGGAGCATTCAAGACATACCTATGATGCCCAGGCGAAGATATGAGATTGCTAATAATTACATGCCAAAAGTTGGCTCGCTTGGACATGATTTGATGTTCAGGATTTGCACAGTACAGGTTAATCTGGACTTCAGTTCTGAAGCAGACATGAAACGTAAAATTCGTGCTAGTCTTGCTTTGCAGCCTATAGCAACAGCCCTGTTTGCAAATTCTCCTTTTACTGAAGGAAAGCCAAATGGTTTTTTGAGCAGGAGAAGTCACATCTATACTGATACTGACAGGAATCGCACTGGCATGCTTCCTTTTGCCTTTGATAACTCCTTTGGGTTTGAGCGGTATGTTGATTATGCTCTTGATGTTCCCATACATATTCATGTCATGGTGTTATCCATGCAGGACTTTATGGCTGGAAAGCTTCATTGCCTTCCGGGTGAATTACCAACCCTGAATGATTGGGAAAATCATTTGGGAACTCTATATCCAGAGGTGGGCTTACTGTATGATGAGGTTTCCCTGCATAATGTGTTAGACATGACAGCTGATTGGACTCCTGAAGAAAGACAGATGCTGAGAGATAAGGTTCCTAAAACAGGTCTAAAGACACCATTCCGTGATGGATTACTCAAGTATGTTGCCGAAGATGTAGTAAAGTTTGCAAAGGATGGCCTGGAAAGAAGAGGCTTTAAGGAATGGGGGTTCTTAAATGAAGCGGTCGAGGTTGTGAGAACAGGCCTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

439

Amino Acids

49.53

Weight (kDa)

6.4

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 87 - 356 6.1e-65 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 650
AccBSI CCGCTC 1 cut(s) 913
AciI CCGC 3 cut(s) 197, 913, 1289
AcsI RAATTY 2 cut(s) 738, 787
AcuI CTGAAG 4 cut(s) 694, 738, 822, 1152
AfaI GTAC 1 cut(s) 693
AfiI CCNNNNNNNGG 3 cut(s) 650, 1067, 1169
AgsI TTSAA 5 cut(s) 159, 292, 473, 560, 590
AjnI CCWGG 3 cut(s) 436, 608, 1245
AloI GAACNNNNNNTCC 4 cut(s) 1042, 1074, 1259, 1291
AluBI AGCT 6 cut(s) 188, 332, 337, 454, 994, 1118
AluI AGCT 6 cut(s) 188, 332, 337, 454, 994, 1118
Alw21I GWGCWC 1 cut(s) 466
Alw44I GTGCAC 1 cut(s) 462
AlwNI CAGNNNCTG 1 cut(s) 1147
AoxI GGCC 3 cut(s) 220, 1243, 1309
ApaLI GTGCAC 1 cut(s) 462
ApeKI GCWGC 3 cut(s) 337, 571, 760
ApoI RAATTY 2 cut(s) 738, 787
AsuC2I CCSGG 1 cut(s) 1008
AsuHPI GGTGA 3 cut(s) 398, 578, 1022
BaeGI GKGCMC 1 cut(s) 466
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 3 cut(s) 324, 583, 772
BccI CCATC 3 cut(s) 231, 1187, 1235
BciT130I CCWGG 3 cut(s) 438, 610, 1247
BciVI GTATCC 1 cut(s) 97
BcnI CCSGG 1 cut(s) 1008
BfaI CTAG 1 cut(s) 747
BfmI CTRYAG 2 cut(s) 37, 765
BfuI GTATCC 1 cut(s) 97
BglII AGATCT 1 cut(s) 211
BisI GCNGC 4 cut(s) 197, 338, 572, 761
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 4 cut(s) 198, 339, 573, 762
Bme1390I CCNGG 4 cut(s) 438, 610, 1008, 1247
BmiI GGNNCC 1 cut(s) 1161
BmrFI CCNGG 4 cut(s) 438, 610, 1008, 1247
BmsI GCATC 5 cut(s) 166, 227, 490, 594, 1134
BpmI CTGGAG 1 cut(s) 459
Bpu10I CCTNAGC 1 cut(s) 61
Bpu1102I GCTNAGC 1 cut(s) 333
BpuEI CTTGAG 3 cut(s) 452, 1187, 1298
BpuMI CCSGG 1 cut(s) 1008
BsaJI CCNNGG 1 cut(s) 608
Bsc4I CCNNNNNNNGG 3 cut(s) 650, 1067, 1169
Bse1I ACTGG 1 cut(s) 874
Bse3DI GCAATG 1 cut(s) 997
BseBI CCWGG 3 cut(s) 438, 610, 1247
BseDI CCNNGG 1 cut(s) 608
BseGI GGATG 2 cut(s) 242, 1246
BseLI CCNNNNNNNGG 3 cut(s) 650, 1067, 1169
BseMI GCAATG 1 cut(s) 997
BseMII CTCAG 3 cut(s) 324, 345, 1139
BseNI ACTGG 1 cut(s) 874
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 3 cut(s) 324, 583, 772
Bsh1285I CGRYCG 1 cut(s) 1293
BshFI GGCC 3 cut(s) 222, 1245, 1311
BsiEI CGRYCG 1 cut(s) 1293
BsiHKAI GWGCWC 1 cut(s) 466
BsiSI CCGG 1 cut(s) 1007
BslFI GGGAC 1 cut(s) 294
BslI CCNNNNNNNGG 3 cut(s) 650, 1067, 1169
BsmFI GGGAC 1 cut(s) 294
BsmI GAATGC 1 cut(s) 585
BsnI GGCC 3 cut(s) 222, 1245, 1311
Bsp1286I GDGCHC 1 cut(s) 466
Bsp143I GATC 1 cut(s) 211
Bsp1720I GCTNAGC 1 cut(s) 333
BspACI CCGC 3 cut(s) 197, 913, 1289
BspANI GGCC 3 cut(s) 222, 1245, 1311
BspCNI CTCAG 3 cut(s) 325, 346, 1140
BspLI GGNNCC 1 cut(s) 1161
BspMAI CTGCAG 1 cut(s) 41
BsrBI CCGCTC 1 cut(s) 913
BsrDI GCAATG 1 cut(s) 997
BsrI ACTGG 1 cut(s) 874
BssECI CCNNGG 1 cut(s) 608
BssMI GATC 1 cut(s) 211
Bst2UI CCWGG 3 cut(s) 438, 610, 1247
Bst4CI ACNGT 3 cut(s) 109, 691, 1077
Bst6I CTCTTC 2 cut(s) 61, 1249
BstC8I GCNNGC 4 cut(s) 242, 416, 656, 875
BstDEI CTNAG 5 cut(s) 61, 333, 354, 455, 1148
BstENI CCTNNNNNAGG 1 cut(s) 1167
BstF5I GGATG 2 cut(s) 242, 1246
BstKTI GATC 1 cut(s) 214
BstMBI GATC 1 cut(s) 211
BstMCI CGRYCG 1 cut(s) 1293
BstMWI GCNNNNNNNGC 4 cut(s) 152, 185, 752, 782
BstNI CCWGG 3 cut(s) 438, 610, 1247
BstNSI RCATGY 2 cut(s) 643, 877
BstSCI CCNGG 4 cut(s) 436, 608, 1006, 1245
BstSFI CTRYAG 2 cut(s) 37, 765
BstSLI GKGCMC 1 cut(s) 466
BstV1I GCAGC 3 cut(s) 324, 583, 772
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BsuI GTATCC 1 cut(s) 97
BsuRI GGCC 3 cut(s) 222, 1245, 1311
BtsCI GGATG 2 cut(s) 242, 1246
BtsIMutI CAGTG 1 cut(s) 867
Cac8I GCNNGC 4 cut(s) 242, 416, 656, 875
CaiI CAGNNNCTG 1 cut(s) 1147
Csp6I GTAC 1 cut(s) 692
CviQI GTAC 1 cut(s) 692
DdeI CTNAG 5 cut(s) 61, 333, 354, 455, 1148
DpnI GATC 1 cut(s) 213
DpnII GATC 1 cut(s) 211
DraI TTTAAA 1 cut(s) 297
Eam1104I CTCTTC 2 cut(s) 61, 1249
EarI CTCTTC 2 cut(s) 61, 1249
Eco147I AGGCCT 1 cut(s) 1311
Eco57I CTGAAG 4 cut(s) 694, 738, 822, 1152
EcoNI CCTNNNNNAGG 1 cut(s) 1167
EcoRII CCWGG 3 cut(s) 436, 608, 1245
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FaqI GGGAC 1 cut(s) 294
Fnu4HI GCNGC 4 cut(s) 197, 338, 572, 761
FokI GGATG 2 cut(s) 249, 1253
Fsp4HI GCNGC 4 cut(s) 197, 338, 572, 761
FspBI CTAG 1 cut(s) 747
GluI GCNGC 4 cut(s) 197, 338, 572, 761
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 3 cut(s) 222, 1245, 1311
HapII CCGG 1 cut(s) 1007
HindIII AAGCTT 1 cut(s) 992
HinfI GANTC 4 cut(s) 402, 503, 862, 1126
HpaII CCGG 1 cut(s) 1007
HphI GGTGA 3 cut(s) 398, 578, 1022
Hpy166II GTNNAC 2 cut(s) 305, 464
Hpy188I TCNGA 1 cut(s) 718
Hpy188III TCNNGA 9 cut(s) 234, 470, 557, 590, 679, 704, 932, 1061, 1130
Hpy8I GTNNAC 2 cut(s) 305, 464
HpyAV CCTTC 3 cut(s) 377, 797, 1013
HpyCH4III ACNGT 3 cut(s) 109, 691, 1077
HpyCH4IV ACGT 3 cut(s) 136, 307, 733
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 185, 752, 782
HpyF3I CTNAG 5 cut(s) 61, 333, 354, 455, 1148
HpySE526I ACGT 3 cut(s) 136, 307, 733
Kzo9I GATC 1 cut(s) 211
LmnI GCTCC 1 cut(s) 582
Lsp1109I GCAGC 3 cut(s) 324, 583, 772
LweI GCATC 5 cut(s) 166, 227, 490, 594, 1134
MaeI CTAG 1 cut(s) 747
MaeII ACGT 3 cut(s) 136, 307, 733
MaeIII GTNAC 1 cut(s) 836
MalI GATC 1 cut(s) 213
MbiI CCGCTC 1 cut(s) 913
MboI GATC 1 cut(s) 211
MboII GAAGA 8 cut(s) 48, 100, 185, 221, 627, 1145, 1229, 1266
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 1 cut(s) 466
MluCI AATT 8 cut(s) 261, 292, 546, 561, 634, 738, 787, 1013
MlyI GAGTC 2 cut(s) 396, 1120
MnlI CCTC 9 cut(s) 78, 241, 273, 434, 477, 1057, 1078, 1250, 1288
MseI TTAA 5 cut(s) 296, 522, 699, 1263, 1280
MslI CAYNNNNRTG 3 cut(s) 599, 872, 951
MspA1I CMGCKG 2 cut(s) 199, 1118
MspI CCGG 1 cut(s) 1007
MspR9I CCNGG 4 cut(s) 438, 610, 1008, 1247
Mva1269I GAATGC 1 cut(s) 585
MvaI CCWGG 3 cut(s) 438, 610, 1247
MwoI GCNNNNNNNGC 4 cut(s) 152, 185, 752, 782
NciI CCSGG 1 cut(s) 1008
NdeII GATC 1 cut(s) 211
NlaIV GGNNCC 1 cut(s) 1161
NmuCI GTSAC 1 cut(s) 836
NspI RCATGY 2 cut(s) 643, 877
PaeI GCATGC 1 cut(s) 877
PceI AGGCCT 1 cut(s) 1311
PcsI WCGNNNNNNNCGW 1 cut(s) 739
PctI GAATGC 1 cut(s) 585
PfeI GAWTC 2 cut(s) 503, 862
PflMI CCANNNNNTGG 1 cut(s) 650
PkrI GCNGC 4 cut(s) 198, 339, 573, 762
PleI GAGTC 2 cut(s) 396, 1120
PpsI GAGTC 2 cut(s) 396, 1120
Psp6I CCWGG 3 cut(s) 436, 608, 1245
PspGI CCWGG 3 cut(s) 436, 608, 1245
PspN4I GGNNCC 1 cut(s) 1161
PstI CTGCAG 1 cut(s) 41
PstNI CAGNNNCTG 1 cut(s) 1147
PsuI RGATCY 1 cut(s) 211
PvuII CAGCTG 1 cut(s) 1118
RsaI GTAC 1 cut(s) 693
RsaNI GTAC 1 cut(s) 692
RseI CAYNNNNRTG 3 cut(s) 599, 872, 951
SaqAI TTAA 5 cut(s) 296, 522, 699, 1263, 1280
SatI GCNGC 4 cut(s) 197, 338, 572, 761
Sau3AI GATC 1 cut(s) 211
SchI GAGTC 2 cut(s) 396, 1120
ScrFI CCNGG 4 cut(s) 438, 610, 1008, 1247
SduI GDGCHC 1 cut(s) 466
SfaNI GCATC 5 cut(s) 166, 227, 490, 594, 1134
SfcI CTRYAG 2 cut(s) 37, 765
SmiMI CAYNNNNRTG 3 cut(s) 599, 872, 951
SmlI CTYRAG 3 cut(s) 431, 1202, 1313
SmoI CTYRAG 3 cut(s) 431, 1202, 1313
SphI GCATGC 1 cut(s) 877
Sse9I AATT 8 cut(s) 261, 292, 546, 561, 634, 738, 787, 1013
SseBI AGGCCT 1 cut(s) 1311
SsiI CCGC 3 cut(s) 197, 913, 1289
SspMI CTAG 1 cut(s) 747
StuI AGGCCT 1 cut(s) 1311
StyD4I CCNGG 4 cut(s) 436, 608, 1006, 1245
TaaI ACNGT 3 cut(s) 109, 691, 1077
TaiI ACGT 3 cut(s) 139, 310, 736
TaqI TCGA 2 cut(s) 501, 1293
TasI AATT 8 cut(s) 261, 292, 546, 561, 634, 738, 787, 1013
TatI WGTACW 1 cut(s) 691
TauI GCSGC 1 cut(s) 199
TfiI GAWTC 2 cut(s) 503, 862
Tru1I TTAA 5 cut(s) 296, 522, 699, 1263, 1280
Tru9I TTAA 5 cut(s) 296, 522, 699, 1263, 1280
TscAI CASTG 1 cut(s) 874
TseFI GTSAC 1 cut(s) 836
TseI GCWGC 3 cut(s) 337, 571, 760
Tsp45I GTSAC 1 cut(s) 836
TspDTI ATGAA 4 cut(s) 743, 941, 986, 1299
TspGWI ACGGA 1 cut(s) 1178
TspRI CASTG 1 cut(s) 874
Van91I CCANNNNNTGG 1 cut(s) 650
VneI GTGCAC 1 cut(s) 462
XagI CCTNNNNNAGG 1 cut(s) 1167
XapI RAATTY 2 cut(s) 738, 787
XceI RCATGY 2 cut(s) 643, 877
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XspI CTAG 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.