MD14G1104000.v1.1

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001519
Physical Location & Seq
Reverse (-)
16131616 .. 16139665
8050 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1104000.v1.1.491

Sequence Viewer

Length: 1563 bp
ATGGGGTCATCATACTGCATCCGTACTGAGCTATTTCGATATAAATCTGGGAAAAATGTGGTTTTCAGTAGGGCAAACAGTATAGATGCATCCAAGTTGAAGGAAAATTCCATTGGCTTCTCTTCTCTATTATATTCTTCTACTAGTAAGGCACAGATTTTGCATATAGCCAATGCAGGAGTAGGACAGAAACGAGGACATAGTGTGATAGTTACTGCCAGTCCTCCAACAGAAGATGCAGTAATTGCTACTGAGCCACTGACAAAAGAGGATCTTGTAGCGTATCTTGCCTCTGGATGCAAGTCTAAGGACAAATGGAGAATTGGCACAGAGCACGAGAAATTTGGTTTTGATCGAAAAACCTTACGTCCTATAAAATATGAACAAATAGCCGAGTTGCTTAATGGAATTTCTGAGAGATATGATTGGGAAAAAGTTATGGAAGGCGACAAAATTATTGGCCTCAAACAGGGTAAGCAAAGCATATCACTGGAGCCTGGAGGTCAATTTGAACTTAGTGGCGCACCTCTTGAGACTCTGCATCAAACTTGTGCCGAAGTCAATTCACACCTTTATCAGGTCAAAGCACTTTCAGAGGAAATGGGAATCGGGTTTCTTGGAATCGGTTTTCAGCCTAAATGGGGACTTAAGGACATACCTATAATGCCCAAGGGAAGATATGATATTATGAGAAATTACATGCCAAAAGTTGGCACTCTTGGACTGGATATGATGTTCAGAACTTGCACTGTACAGGTTAATCTGGACTTCAGTTCTGAAGCAGACATGATAAGGAAATTTCGTGCTGGTCTTGCTTTACAGCCTATAGCAACAGCTCTATTCGCAAATTCACCTTTCACTGAAGGAAAGCCAAATGGTTTTCTAAGCATGAGAAGCCAAATTTGGACCGATACGGATAATAATCGCGCTGGCATGCTTCCTTTTGTTTTTGATGACTCTTTTGGGTTTGAGCAGTATGTTGATTATGCTCTTGATGTTCCCATGTACTTTGTTTATCGGCAAAAGAAGTATATCGATTGCACTGGAATGAGCTTCCGGGACTTTTTGGCAGGGAAGCTTCCTTGCATTCCGGGTGAATTGCCAACCCTGAACGATTGGGAGAATCATTTGACAACCATATTCCCTGAGGTCAGGCTAAAGAGATACTTGGAGATGAGGGGTGCTGATGGAGGGCCTTGGAGGAGATTATGTGCTTTACCCGCCTTTTGGGTAGGTATATTGTACGACGAGGTTTCCCTACAAAACGTATTAGACCTTACAGCTGATTGGACTCCTGAAGAAAGACAGTTGCTGAGAAATAAGGTCCCCATAACTGGTCTAAAGACGCCATTTAGGGATGGATTGCTCAAGCATGTAGCTCAAGATGTTGTAATGTTGGCAAAGGATGGCCTGGAAAGGAGAGGTTTCAAAGAAACAGGATTCTTGAATGAGGTGGTGGAGTTGGTTAGAGCTGGTGTAACACCAGCTGAGAAGCTATTGGAGTTGTACAATGGGAAATGGGGACAACAGATAGACCCCGTTTTTGAAGAGCTACTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

521

Amino Acids

58.65

Weight (kDa)

6.45

Isoelectric Point (pI)

34.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 107 - 516 2.7e-105 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 710
AccII CGCG 1 cut(s) 927
AciI CCGC 1 cut(s) 1221
AclWI GGATC 1 cut(s) 279
AcsI RAATTY 6 cut(s) 106, 341, 408, 797, 847, 900
AcuI CTGAAG 4 cut(s) 754, 798, 882, 1317
AcyI GRCGYC 1 cut(s) 1346
AfaI GTAC 5 cut(s) 25, 753, 1007, 1244, 1508
AfiI CCNNNNNNNGG 6 cut(s) 469, 577, 641, 710, 1227, 1334
AflII CTTAAG 1 cut(s) 647
AgsI TTSAA 5 cut(s) 100, 512, 1429, 1447, 1547
AhlI ACTAGT 1 cut(s) 143
AjnI CCWGG 2 cut(s) 496, 1410
AjuI GAANNNNNNNTTGG 4 cut(s) 96, 128, 886, 918
AloI GAACNNNNNNTCC 2 cut(s) 719, 751
Alw21I GWGCWC 1 cut(s) 336
Alw26I GTCTC 1 cut(s) 527
AlwI GGATC 1 cut(s) 279
AlwNI CAGNNNCTG 1 cut(s) 1312
AoxI GGCC 3 cut(s) 460, 1193, 1408
ApoI RAATTY 6 cut(s) 106, 341, 408, 797, 847, 900
ArsI GACNNNNNNTTYG 2 cut(s) 1526, 1558
AspLEI GCGC 2 cut(s) 524, 929
AspS9I GGNCC 3 cut(s) 906, 1193, 1324
AsuC2I CCSGG 2 cut(s) 1058, 1092
AsuHPI GGTGA 2 cut(s) 843, 1106
AvaII GGWCC 2 cut(s) 906, 1324
AxyI CCTNAGG 1 cut(s) 1146
BauI CACGAG 1 cut(s) 335
Bbv12I GWGCWC 1 cut(s) 336
BccI CCATC 3 cut(s) 1181, 1352, 1400
BciT130I CCWGG 2 cut(s) 498, 1412
BcnI CCSGG 2 cut(s) 1058, 1092
BcoDI GTCTC 1 cut(s) 527
BcuI ACTAGT 1 cut(s) 143
BfaI CTAG 1 cut(s) 144
BfmI CTRYAG 1 cut(s) 825
BfrI CTTAAG 1 cut(s) 647
Bme1390I CCNGG 4 cut(s) 498, 1058, 1092, 1412
Bme18I GGWCC 2 cut(s) 906, 1324
BmgT120I GGNCC 3 cut(s) 906, 1193, 1324
BmiI GGNNCC 2 cut(s) 495, 1326
BmrFI CCNGG 4 cut(s) 498, 1058, 1092, 1412
BmsI GCATC 6 cut(s) 27, 76, 98, 226, 287, 550
BpmI CTGGAG 2 cut(s) 512, 519
BpuEI CTTGAG 3 cut(s) 551, 1352, 1365
BpuMI CCSGG 2 cut(s) 1058, 1092
Bsa29I ATCGAT 1 cut(s) 1035
BsaBI GATNNNNATC 2 cut(s) 43, 921
BsaHI GRCGYC 1 cut(s) 1346
BsaJI CCNNGG 2 cut(s) 669, 1196
BsaXI ACNNNNNCTCC 2 cut(s) 1195, 1225
Bsc4I CCNNNNNNNGG 6 cut(s) 469, 577, 641, 710, 1227, 1334
Bse1I ACTGG 5 cut(s) 219, 495, 729, 1048, 1339
Bse21I CCTNAGG 1 cut(s) 1146
Bse8I GATNNNNATC 2 cut(s) 43, 921
BseBI CCWGG 2 cut(s) 498, 1412
BseCI ATCGAT 1 cut(s) 1035
BseDI CCNNGG 2 cut(s) 669, 1196
BseGI GGATG 5 cut(s) 18, 89, 302, 1363, 1411
BseJI GATNNNNATC 2 cut(s) 43, 921
BseLI CCNNNNNNNGG 6 cut(s) 469, 577, 641, 710, 1227, 1334
BseMII CTCAG 6 cut(s) 18, 243, 405, 1137, 1304, 1479
BseNI ACTGG 5 cut(s) 219, 495, 729, 1048, 1339
BseRI GAGGAG 1 cut(s) 1216
Bsh1236I CGCG 1 cut(s) 927
BshFI GGCC 3 cut(s) 462, 1195, 1410
BshVI ATCGAT 1 cut(s) 1035
BsiHKAI GWGCWC 1 cut(s) 336
BsiSI CCGG 2 cut(s) 1057, 1091
BslFI GGGAC 4 cut(s) 657, 1073, 1310, 1536
BslI CCNNNNNNNGG 6 cut(s) 469, 577, 641, 710, 1227, 1334
BsmAI GTCTC 1 cut(s) 527
BsmFI GGGAC 4 cut(s) 657, 1073, 1310, 1536
BsmI GAATGC 1 cut(s) 1086
BsnI GGCC 3 cut(s) 462, 1195, 1410
Bsp1286I GDGCHC 1 cut(s) 336
Bsp1407I TGTACA 2 cut(s) 751, 1506
Bsp143I GATC 2 cut(s) 271, 352
BspACI CCGC 1 cut(s) 1221
BspANI GGCC 3 cut(s) 462, 1195, 1410
BspCNI CTCAG 6 cut(s) 19, 244, 406, 1138, 1305, 1480
BspDI ATCGAT 1 cut(s) 1035
BspFNI CGCG 1 cut(s) 927
BspLI GGNNCC 2 cut(s) 495, 1326
BspPI GGATC 1 cut(s) 279
BspQI GCTCTTC 1 cut(s) 1542
BspTI CTTAAG 1 cut(s) 647
BsrGI TGTACA 2 cut(s) 751, 1506
BsrI ACTGG 5 cut(s) 219, 495, 729, 1048, 1339
BssECI CCNNGG 2 cut(s) 669, 1196
BssMI GATC 2 cut(s) 271, 352
BssNI GRCGYC 1 cut(s) 1346
BssSI CACGAG 1 cut(s) 335
BssT1I CCWWGG 2 cut(s) 669, 1196
Bst2BI CACGAG 1 cut(s) 335
Bst2UI CCWGG 2 cut(s) 498, 1412
Bst4CI ACNGT 3 cut(s) 80, 751, 1308
Bst6I CTCTTC 2 cut(s) 127, 1542
BstACI GRCGYC 1 cut(s) 1346
BstAFI CTTAAG 1 cut(s) 647
BstAPI GCANNNNNTGC 1 cut(s) 245
BstAUI TGTACA 2 cut(s) 751, 1506
BstC8I GCNNGC 2 cut(s) 931, 935
BstDEI CTNAG 9 cut(s) 27, 252, 306, 414, 515, 884, 1146, 1313, 1488
BstENI CCTNNNNNAGG 2 cut(s) 467, 575
BstF5I GGATG 5 cut(s) 18, 89, 302, 1363, 1411
BstFNI CGCG 1 cut(s) 927
BstHHI GCGC 2 cut(s) 524, 929
BstKTI GATC 2 cut(s) 274, 355
BstMAI GTCTC 1 cut(s) 527
BstMBI GATC 2 cut(s) 271, 352
BstMWI GCNNNNNNNGC 6 cut(s) 245, 287, 812, 842, 894, 1220
BstNI CCWGG 2 cut(s) 498, 1412
BstNSI RCATGY 3 cut(s) 703, 937, 1376
BstSCI CCNGG 4 cut(s) 496, 1056, 1090, 1410
BstSFI CTRYAG 1 cut(s) 825
BstUI CGCG 1 cut(s) 927
BstX2I RGATCY 1 cut(s) 271
BstYI RGATCY 1 cut(s) 271
Bsu15I ATCGAT 1 cut(s) 1035
Bsu36I CCTNAGG 1 cut(s) 1146
BsuRI GGCC 3 cut(s) 462, 1195, 1410
BsuTUI ATCGAT 1 cut(s) 1035
BtsCI GGATG 5 cut(s) 18, 89, 302, 1363, 1411
BtsIMutI CAGTG 5 cut(s) 257, 488, 747, 858, 1041
Cac8I GCNNGC 2 cut(s) 931, 935
CaiI CAGNNNCTG 1 cut(s) 1312
CfoI GCGC 2 cut(s) 524, 929
Cfr13I GGNCC 3 cut(s) 906, 1193, 1324
ClaI ATCGAT 1 cut(s) 1035
CseI GACGC 1 cut(s) 1354
Csp6I GTAC 5 cut(s) 24, 752, 1006, 1243, 1507
CviAII CATG 6 cut(s) 700, 787, 889, 934, 1003, 1373
CviQI GTAC 5 cut(s) 24, 752, 1006, 1243, 1507
DdeI CTNAG 9 cut(s) 27, 252, 306, 414, 515, 884, 1146, 1313, 1488
DpnI GATC 2 cut(s) 273, 354
DpnII GATC 2 cut(s) 271, 352
Eam1104I CTCTTC 2 cut(s) 127, 1542
EarI CTCTTC 2 cut(s) 127, 1542
Eco130I CCWWGG 2 cut(s) 669, 1196
Eco47I GGWCC 2 cut(s) 906, 1324
Eco57I CTGAAG 4 cut(s) 754, 798, 882, 1317
Eco81I CCTNAGG 1 cut(s) 1146
EcoNI CCTNNNNNAGG 2 cut(s) 467, 575
EcoO109I RGGNCCY 2 cut(s) 1193, 1324
EcoRII CCWGG 2 cut(s) 496, 1410
EcoT14I CCWWGG 2 cut(s) 669, 1196
EcoT22I ATGCAT 1 cut(s) 91
ErhI CCWWGG 2 cut(s) 669, 1196
FaeI CATG 6 cut(s) 703, 790, 892, 937, 1006, 1376
FalI AAGNNNNNCTT 4 cut(s) 258, 290, 1151, 1183
FaqI GGGAC 4 cut(s) 657, 1073, 1310, 1536
FatI CATG 6 cut(s) 699, 786, 888, 933, 1002, 1372
FauI CCCGC 1 cut(s) 1228
FokI GGATG 5 cut(s) 5, 76, 309, 1370, 1418
FspBI CTAG 1 cut(s) 144
GlaI GCGC 2 cut(s) 523, 928
GsuI CTGGAG 2 cut(s) 512, 519
HaeIII GGCC 3 cut(s) 462, 1195, 1410
HapII CCGG 2 cut(s) 1057, 1091
HgaI GACGC 1 cut(s) 1354
HhaI GCGC 2 cut(s) 524, 929
Hin1I GRCGYC 1 cut(s) 1346
Hin1II CATG 6 cut(s) 703, 790, 892, 937, 1006, 1376
Hin6I GCGC 2 cut(s) 522, 927
HinP1I GCGC 2 cut(s) 522, 927
HindIII AAGCTT 1 cut(s) 1076
HinfI GANTC 7 cut(s) 535, 606, 621, 956, 1123, 1291, 1440
HpaII CCGG 2 cut(s) 1057, 1091
HphI GGTGA 2 cut(s) 843, 1106
Hpy188I TCNGA 4 cut(s) 415, 595, 740, 778
Hpy188III TCNNGA 7 cut(s) 294, 530, 764, 992, 1295, 1382, 1444
Hpy99I CGWCG 1 cut(s) 1250
HpyAV CCTTC 3 cut(s) 94, 437, 857
HpyCH4III ACNGT 3 cut(s) 80, 751, 1308
HpyCH4IV ACGT 2 cut(s) 367, 1266
HpyF10VI GCNNNNNNNGC 6 cut(s) 245, 287, 812, 842, 894, 1220
HpyF3I CTNAG 9 cut(s) 27, 252, 306, 414, 515, 884, 1146, 1313, 1488
HpySE526I ACGT 2 cut(s) 367, 1266
Hsp92I GRCGYC 1 cut(s) 1346
Hsp92II CATG 6 cut(s) 703, 790, 892, 937, 1006, 1376
HspAI GCGC 2 cut(s) 522, 927
Kzo9I GATC 2 cut(s) 271, 352
LguI GCTCTTC 1 cut(s) 1542
LmnI GCTCC 1 cut(s) 493
LweI GCATC 6 cut(s) 27, 76, 98, 226, 287, 550
MaeI CTAG 1 cut(s) 144
MaeII ACGT 2 cut(s) 367, 1266
MaeIII GTNAC 2 cut(s) 211, 1477
MalI GATC 2 cut(s) 273, 354
MboI GATC 2 cut(s) 271, 352
MboII GAAGA 6 cut(s) 114, 129, 245, 687, 1310, 1559
MflI RGATCY 1 cut(s) 271
MhlI GDGCHC 1 cut(s) 336
MlyI GAGTC 3 cut(s) 529, 950, 1285
MmeI TCCRAC 1 cut(s) 251
Mph1103I ATGCAT 1 cut(s) 91
MseI TTAA 3 cut(s) 402, 648, 759
MslI CAYNNNNRTG 1 cut(s) 1046
MspA1I CMGCKG 2 cut(s) 1283, 1487
MspCI CTTAAG 1 cut(s) 647
MspI CCGG 2 cut(s) 1057, 1091
MspR9I CCNGG 4 cut(s) 498, 1058, 1092, 1412
Mva1269I GAATGC 1 cut(s) 1086
MvaI CCWGG 2 cut(s) 498, 1412
MvnI CGCG 1 cut(s) 927
MwoI GCNNNNNNNGC 6 cut(s) 245, 287, 812, 842, 894, 1220
NciI CCSGG 2 cut(s) 1058, 1092
NdeII GATC 2 cut(s) 271, 352
NlaIII CATG 6 cut(s) 703, 790, 892, 937, 1006, 1376
NlaIV GGNNCC 2 cut(s) 495, 1326
NmeAIII GCCGAG 1 cut(s) 418
NsiI ATGCAT 1 cut(s) 91
NspI RCATGY 3 cut(s) 703, 937, 1376
PaeI GCATGC 1 cut(s) 937
PciSI GCTCTTC 1 cut(s) 1542
PctI GAATGC 1 cut(s) 1086
PfeI GAWTC 4 cut(s) 606, 621, 1123, 1440
PflMI CCANNNNNTGG 1 cut(s) 710
PfoI TCCNGGA 1 cut(s) 1056
PleI GAGTC 3 cut(s) 529, 950, 1285
PpsI GAGTC 3 cut(s) 529, 950, 1285
PpuMI RGGWCCY 1 cut(s) 1324
Psp5II RGGWCCY 1 cut(s) 1324
Psp6I CCWGG 2 cut(s) 496, 1410
PspGI CCWGG 2 cut(s) 496, 1410
PspN4I GGNNCC 2 cut(s) 495, 1326
PspPI GGNCC 3 cut(s) 906, 1193, 1324
PspPPI RGGWCCY 1 cut(s) 1324
PstNI CAGNNNCTG 1 cut(s) 1312
PsuI RGATCY 1 cut(s) 271
PvuII CAGCTG 2 cut(s) 1283, 1487
RsaI GTAC 5 cut(s) 25, 753, 1007, 1244, 1508
RsaNI GTAC 5 cut(s) 24, 752, 1006, 1243, 1507
RseI CAYNNNNRTG 1 cut(s) 1046
SapI GCTCTTC 1 cut(s) 1542
SaqAI TTAA 3 cut(s) 402, 648, 759
Sau3AI GATC 2 cut(s) 271, 352
Sau96I GGNCC 3 cut(s) 906, 1193, 1324
SchI GAGTC 3 cut(s) 529, 950, 1285
ScrFI CCNGG 4 cut(s) 498, 1058, 1092, 1412
SduI GDGCHC 1 cut(s) 336
SfaNI GCATC 6 cut(s) 27, 76, 98, 226, 287, 550
SfcI CTRYAG 1 cut(s) 825
SinI GGWCC 2 cut(s) 906, 1324
SmiMI CAYNNNNRTG 1 cut(s) 1046
SmlI CTYRAG 4 cut(s) 530, 647, 1367, 1380
SmoI CTYRAG 4 cut(s) 530, 647, 1367, 1380
SpeI ACTAGT 1 cut(s) 143
SphI GCATGC 1 cut(s) 937
SsiI CCGC 1 cut(s) 1221
SspMI CTAG 1 cut(s) 144
StyD4I CCNGG 4 cut(s) 496, 1056, 1090, 1410
StyI CCWWGG 2 cut(s) 669, 1196
TaaI ACNGT 3 cut(s) 80, 751, 1308
TaiI ACGT 2 cut(s) 370, 1269
TaqI TCGA 3 cut(s) 37, 355, 1035
TaqII GACCGA 1 cut(s) 923
TatI WGTACW 3 cut(s) 751, 1005, 1506
TfiI GAWTC 4 cut(s) 606, 621, 1123, 1440
Tru1I TTAA 3 cut(s) 402, 648, 759
Tru9I TTAA 3 cut(s) 402, 648, 759
TscAI CASTG 5 cut(s) 264, 495, 754, 865, 1048
TspDTI ATGAA 1 cut(s) 396
TspGWI ACGGA 2 cut(s) 11, 929
TspRI CASTG 5 cut(s) 264, 495, 754, 865, 1048
Van91I CCANNNNNTGG 1 cut(s) 710
Vha464I CTTAAG 1 cut(s) 647
VpaK11BI GGWCC 2 cut(s) 906, 1324
XagI CCTNNNNNAGG 2 cut(s) 467, 575
XapI RAATTY 6 cut(s) 106, 341, 408, 797, 847, 900
XceI RCATGY 3 cut(s) 703, 937, 1376
XspI CTAG 1 cut(s) 144
Zsp2I ATGCAT 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.