pycom06g07910

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001519
Physical Location & Seq
Reverse (-)
12891347 .. 12893954
2608 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g07910.4

Sequence Viewer

Length: 1212 bp
ATGACATACGAACACATTGCTAAGTTGCTTAATGGGATTGCTGAGAGGTTTGATTGGGAAAAAATTATGGAAGGGGACAACATTATTGCACTTAAACAGGGTAAGCAAAGCATAACGCTGGAGCCGGGAGGTCAATTCGAGCATAGTGGTGCACCTCTTGAAACTCTGCATCAAATTTGTGCTGAAGTCAATTCACATCTTTATCAGGTTAAAGAAGTTCTAAACGAAATGGGAATCGGATTGATGGATATTGGTTGTCAGCCAAAGTGGGAAGTTAAAGACATACCTATGATGCCTAAGGTATTGTTGAACTTTGGTTACATATCAAAGACTCCTTTTCAAATTCATTCTGCCATTTTGTATTTTAATTACTTACATACTGATCGAGTATATTTTGGCTCAGGAAAGATACGATCGATCATTAGGAATTACATGCCCAAAGTTGGCTCTCTTGGACTTGATTTGATGTTCAGGACTTGCACTGCACAGGTTAATCTGGACTTTAGTTCTGAAGCGGACATGACAAAGAAACTTCGCGCTAGTCTTGCTTTGCAGCCTGTGAGAACACTGAACTTGTTTTCGGTGTACATTTTTCTGCTTATATGGTATCTAACTTTTCAACAAATGGAACAGATTGCAACAGCTCTATTTGCAAATTCACCTTTCACTGAAGGAAAGCCAAATGGTTTTCTTAGCATGAGAAGGTTCGAACAGTATGTTGAATATGCTCTCGATATCCCCATGTACTTCGTTTATCAGAACAAGAGGCATTTTGACTGTACTGGGATGACCTTCCGGGTTAGGCTGAAGAGATACTTGGAGATGAGGGGTGCTGATGGAGGACCGTGGAGTAGATTACAAGCTTTGCCTGCCTTTTGGGTTGGTTTGTTGTATGATGAGGTTTCCTTACAAAATGTACTAGACCTGATAGCCGACTGGACAGCTGAAGAAAGACAGATGCTGAGAAATCAGGTCCCTAAAACTGGTCTAATGACACCATTTCGTGGCCGATTGCTAAAGCATGTTGCTGAAGATGTGGTACAATTCGCAAAGGCTGGCCTGGAAAGAAGAGGCTTTAATGAATCACGGTTTTTGAATGACTTGGTGGAGGTGGCTAGAACTGGTGTAACACCAGCAGAGAAGCTGTTGGAGTTGTATTACAAGAAATGGGGACAAAATATAGAGCCTATCTTTGAGGAGCTACTAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

404

Amino Acids

46.55

Weight (kDa)

7.09

Isoelectric Point (pI)

37.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1004
AccII CGCG 1 cut(s) 537
AciI CCGC 1 cut(s) 515
AcoI YGGCCR 1 cut(s) 1006
AcsI RAATTY 3 cut(s) 174, 342, 655
AcuI CTGAAG 6 cut(s) 204, 531, 690, 827, 966, 1050
AfaI GTAC 5 cut(s) 587, 746, 781, 918, 1041
AfiI CCNNNNNNNGG 3 cut(s) 443, 983, 1004
AgsI TTSAA 6 cut(s) 161, 310, 341, 620, 722, 1096
AjnI CCWGG 1 cut(s) 1059
AjuI GAANNNNNNNTTGG 2 cut(s) 800, 832
AluBI AGCT 6 cut(s) 644, 863, 944, 1144, 1201, 1209
AluI AGCT 6 cut(s) 644, 863, 944, 1144, 1201, 1209
Alw21I GWGCWC 1 cut(s) 154
Alw44I GTGCAC 1 cut(s) 150
AlwNI CAGNNNCTG 1 cut(s) 961
AoxI GGCC 2 cut(s) 1006, 1057
ApaLI GTGCAC 1 cut(s) 150
ApeKI GCWGC 1 cut(s) 553
ApoI RAATTY 3 cut(s) 174, 342, 655
AspLEI GCGC 1 cut(s) 539
AspS9I GGNCC 2 cut(s) 842, 973
AsuC2I CCSGG 2 cut(s) 126, 797
AsuHPI GGTGA 1 cut(s) 651
AsuII TTCGAA 1 cut(s) 708
AvaII GGWCC 2 cut(s) 842, 973
AxyI CCTNAGG 1 cut(s) 297
BaeGI GKGCMC 1 cut(s) 154
BarI GAAGNNNNNNTAC 2 cut(s) 1023, 1055
Bbv12I GWGCWC 1 cut(s) 154
BbvI GCAGC 1 cut(s) 565
BccI CCATC 2 cut(s) 238, 830
BcgI CGANNNNNNTGC 1 cut(s) 33
BciT130I CCWGG 1 cut(s) 1061
BcnI CCSGG 2 cut(s) 126, 797
BfaI CTAG 3 cut(s) 540, 920, 1116
BisI GCNGC 1 cut(s) 554
BlsI GCNGC 1 cut(s) 555
Bme1390I CCNGG 3 cut(s) 126, 797, 1061
Bme18I GGWCC 2 cut(s) 842, 973
BmgT120I GGNCC 2 cut(s) 842, 973
BmiI GGNNCC 2 cut(s) 123, 975
BmrFI CCNGG 3 cut(s) 126, 797, 1061
BmrI ACTGGG 1 cut(s) 792
BmsI GCATC 3 cut(s) 178, 282, 948
BmuI ACTGGG 1 cut(s) 792
BpmI CTGGAG 1 cut(s) 140
Bpu10I CCTNAGC 1 cut(s) 400
Bpu14I TTCGAA 1 cut(s) 708
BpuMI CCSGG 2 cut(s) 126, 797
Bsa29I ATCGAT 1 cut(s) 416
BsaJI CCNNGG 1 cut(s) 845
Bsc4I CCNNNNNNNGG 3 cut(s) 443, 983, 1004
Bse1I ACTGG 4 cut(s) 787, 941, 988, 1126
Bse21I CCTNAGG 1 cut(s) 297
Bse3DI GCAATG 1 cut(s) 15
BseBI CCWGG 1 cut(s) 1061
BseCI ATCGAT 1 cut(s) 416
BseDI CCNNGG 1 cut(s) 845
BseGI GGATG 1 cut(s) 792
BseLI CCNNNNNNNGG 3 cut(s) 443, 983, 1004
BseMI GCAATG 1 cut(s) 15
BseMII CTCAG 3 cut(s) 33, 414, 953
BseNI ACTGG 4 cut(s) 787, 941, 988, 1126
BseRI GAGGAG 1 cut(s) 1211
BseSI GKGCMC 1 cut(s) 154
BseXI GCAGC 1 cut(s) 565
BsgI GTGCAG 1 cut(s) 468
Bsh1236I CGCG 1 cut(s) 537
Bsh1285I CGRYCG 1 cut(s) 416
BshFI GGCC 2 cut(s) 1008, 1059
BshVI ATCGAT 1 cut(s) 416
BsiEI CGRYCG 1 cut(s) 416
BsiHKAI GWGCWC 1 cut(s) 154
BsiSI CCGG 2 cut(s) 125, 796
BslFI GGGAC 3 cut(s) 89, 959, 1185
BslI CCNNNNNNNGG 3 cut(s) 443, 983, 1004
BsmFI GGGAC 3 cut(s) 89, 959, 1185
BsnI GGCC 2 cut(s) 1008, 1059
Bsp119I TTCGAA 1 cut(s) 708
Bsp1286I GDGCHC 1 cut(s) 154
Bsp1407I TGTACA 1 cut(s) 585
Bsp143I GATC 3 cut(s) 382, 413, 417
BspACI CCGC 1 cut(s) 515
BspANI GGCC 2 cut(s) 1008, 1059
BspCNI CTCAG 3 cut(s) 34, 413, 954
BspDI ATCGAT 1 cut(s) 416
BspFNI CGCG 1 cut(s) 537
BspLI GGNNCC 2 cut(s) 123, 975
BspT104I TTCGAA 1 cut(s) 708
BsrDI GCAATG 1 cut(s) 15
BsrGI TGTACA 1 cut(s) 585
BsrI ACTGG 4 cut(s) 787, 941, 988, 1126
BssECI CCNNGG 1 cut(s) 845
BssMI GATC 3 cut(s) 382, 413, 417
Bst2UI CCWGG 1 cut(s) 1061
Bst4CI ACNGT 4 cut(s) 714, 779, 846, 1089
Bst6I CTCTTC 2 cut(s) 803, 1063
BstAUI TGTACA 1 cut(s) 585
BstBI TTCGAA 1 cut(s) 708
BstC8I GCNNGC 2 cut(s) 870, 1057
BstDEI CTNAG 7 cut(s) 21, 42, 297, 400, 692, 962, 1205
BstDSI CCRYGG 1 cut(s) 845
BstF5I GGATG 1 cut(s) 792
BstFNI CGCG 1 cut(s) 537
BstHHI GCGC 1 cut(s) 539
BstKTI GATC 3 cut(s) 385, 416, 420
BstMBI GATC 3 cut(s) 382, 413, 417
BstMCI CGRYCG 1 cut(s) 416
BstMWI GCNNNNNNNGC 3 cut(s) 545, 650, 869
BstNI CCWGG 1 cut(s) 1061
BstNSI RCATGY 2 cut(s) 436, 1025
BstSCI CCNGG 3 cut(s) 124, 795, 1059
BstSLI GKGCMC 1 cut(s) 154
BstUI CGCG 1 cut(s) 537
BstV1I GCAGC 1 cut(s) 565
Bsu15I ATCGAT 1 cut(s) 416
Bsu36I CCTNAGG 1 cut(s) 297
BsuRI GGCC 2 cut(s) 1008, 1059
BsuTUI ATCGAT 1 cut(s) 416
BtgI CCRYGG 1 cut(s) 845
BtsCI GGATG 1 cut(s) 792
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 3 cut(s) 480, 566, 666
Cac8I GCNNGC 2 cut(s) 870, 1057
CaiI CAGNNNCTG 1 cut(s) 961
CfoI GCGC 1 cut(s) 539
Cfr13I GGNCC 2 cut(s) 842, 973
ClaI ATCGAT 1 cut(s) 416
Csp6I GTAC 5 cut(s) 586, 745, 780, 917, 1040
CviAII CATG 5 cut(s) 433, 520, 697, 742, 1022
CviQI GTAC 5 cut(s) 586, 745, 780, 917, 1040
DdeI CTNAG 7 cut(s) 21, 42, 297, 400, 692, 962, 1205
DpnI GATC 3 cut(s) 384, 415, 419
DpnII GATC 3 cut(s) 382, 413, 417
EaeI YGGCCR 1 cut(s) 1006
Eam1104I CTCTTC 2 cut(s) 803, 1063
EarI CTCTTC 2 cut(s) 803, 1063
Eco32I GATATC 1 cut(s) 736
Eco47I GGWCC 2 cut(s) 842, 973
Eco57I CTGAAG 6 cut(s) 204, 531, 690, 827, 966, 1050
Eco81I CCTNAGG 1 cut(s) 297
EcoO109I RGGNCCY 1 cut(s) 973
EcoRII CCWGG 1 cut(s) 1059
EcoRV GATATC 1 cut(s) 736
FaeI CATG 5 cut(s) 436, 523, 700, 745, 1025
FalI AAGNNNNNCTT 2 cut(s) 800, 832
FaqI GGGAC 3 cut(s) 89, 959, 1185
FatI CATG 5 cut(s) 432, 519, 696, 741, 1021
Fnu4HI GCNGC 1 cut(s) 554
FokI GGATG 1 cut(s) 799
Fsp4HI GCNGC 1 cut(s) 554
FspBI CTAG 3 cut(s) 540, 920, 1116
GlaI GCGC 1 cut(s) 538
GluI GCNGC 1 cut(s) 554
GsuI CTGGAG 1 cut(s) 140
HaeIII GGCC 2 cut(s) 1008, 1059
HapII CCGG 2 cut(s) 125, 796
HhaI GCGC 1 cut(s) 539
Hin1II CATG 5 cut(s) 436, 523, 700, 745, 1025
Hin6I GCGC 1 cut(s) 537
HinP1I GCGC 1 cut(s) 537
HindIII AAGCTT 1 cut(s) 861
HinfI GANTC 3 cut(s) 234, 331, 1082
HpaII CCGG 2 cut(s) 125, 796
HphI GGTGA 1 cut(s) 651
Hpy166II GTNNAC 2 cut(s) 152, 586
Hpy188I TCNGA 3 cut(s) 239, 511, 759
Hpy188III TCNNGA 5 cut(s) 158, 402, 472, 497, 731
Hpy8I GTNNAC 2 cut(s) 152, 586
HpyAV CCTTC 4 cut(s) 65, 665, 696, 802
HpyCH4III ACNGT 4 cut(s) 714, 779, 846, 1089
HpyCH4V TGCA 8 cut(s) 89, 152, 169, 480, 485, 553, 638, 653
HpyF10VI GCNNNNNNNGC 3 cut(s) 545, 650, 869
HpyF3I CTNAG 7 cut(s) 21, 42, 297, 400, 692, 962, 1205
Hsp92II CATG 5 cut(s) 436, 523, 700, 745, 1025
HspAI GCGC 1 cut(s) 537
Kzo9I GATC 3 cut(s) 382, 413, 417
LmnI GCTCC 2 cut(s) 121, 1198
Lsp1109I GCAGC 1 cut(s) 565
LweI GCATC 3 cut(s) 178, 282, 948
MaeI CTAG 3 cut(s) 540, 920, 1116
MaeIII GTNAC 2 cut(s) 317, 1126
MalI GATC 3 cut(s) 384, 415, 419
MboI GATC 3 cut(s) 382, 413, 417
MboII GAAGA 4 cut(s) 820, 959, 1043, 1080
MhlI GDGCHC 1 cut(s) 154
MluCI AATT 9 cut(s) 63, 134, 174, 190, 342, 367, 427, 655, 1043
MlyI GAGTC 1 cut(s) 325
MmeI TCCRAC 1 cut(s) 1128
MseI TTAA 7 cut(s) 30, 93, 210, 276, 366, 492, 1077
MslI CAYNNNNRTG 2 cut(s) 147, 287
MspA1I CMGCKG 1 cut(s) 944
MspI CCGG 2 cut(s) 125, 796
MspR9I CCNGG 3 cut(s) 126, 797, 1061
MvaI CCWGG 1 cut(s) 1061
MvnI CGCG 1 cut(s) 537
MwoI GCNNNNNNNGC 3 cut(s) 545, 650, 869
NciI CCSGG 2 cut(s) 126, 797
NdeII GATC 3 cut(s) 382, 413, 417
NlaIII CATG 5 cut(s) 436, 523, 700, 745, 1025
NlaIV GGNNCC 2 cut(s) 123, 975
NspI RCATGY 2 cut(s) 436, 1025
NspV TTCGAA 1 cut(s) 708
PfeI GAWTC 2 cut(s) 234, 1082
PflMI CCANNNNNTGG 1 cut(s) 1004
PkrI GCNGC 1 cut(s) 555
Ple19I CGATCG 1 cut(s) 416
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
PpuMI RGGWCCY 1 cut(s) 973
Psp5II RGGWCCY 1 cut(s) 973
Psp6I CCWGG 1 cut(s) 1059
PspGI CCWGG 1 cut(s) 1059
PspN4I GGNNCC 2 cut(s) 123, 975
PspPI GGNCC 2 cut(s) 842, 973
PspPPI RGGWCCY 1 cut(s) 973
PsrI GAACNNNNNNTAC 2 cut(s) 302, 334
PstNI CAGNNNCTG 1 cut(s) 961
PvuI CGATCG 1 cut(s) 416
PvuII CAGCTG 1 cut(s) 944
RsaI GTAC 5 cut(s) 587, 746, 781, 918, 1041
RsaNI GTAC 5 cut(s) 586, 745, 780, 917, 1040
RseI CAYNNNNRTG 2 cut(s) 147, 287
SaqAI TTAA 7 cut(s) 30, 93, 210, 276, 366, 492, 1077
SatI GCNGC 1 cut(s) 554
Sau3AI GATC 3 cut(s) 382, 413, 417
Sau96I GGNCC 2 cut(s) 842, 973
SchI GAGTC 1 cut(s) 325
ScrFI CCNGG 3 cut(s) 126, 797, 1061
SduI GDGCHC 1 cut(s) 154
SfaNI GCATC 3 cut(s) 178, 282, 948
SfuI TTCGAA 1 cut(s) 708
SinI GGWCC 2 cut(s) 842, 973
SmiMI CAYNNNNRTG 2 cut(s) 147, 287
Sse9I AATT 9 cut(s) 63, 134, 174, 190, 342, 367, 427, 655, 1043
SsiI CCGC 1 cut(s) 515
SspMI CTAG 3 cut(s) 540, 920, 1116
StyD4I CCNGG 3 cut(s) 124, 795, 1059
TaaI ACNGT 4 cut(s) 714, 779, 846, 1089
TaqI TCGA 5 cut(s) 138, 385, 416, 708, 732
TasI AATT 9 cut(s) 63, 134, 174, 190, 342, 367, 427, 655, 1043
TatI WGTACW 4 cut(s) 585, 744, 779, 916
TfiI GAWTC 2 cut(s) 234, 1082
Tru1I TTAA 7 cut(s) 30, 93, 210, 276, 366, 492, 1077
Tru9I TTAA 7 cut(s) 30, 93, 210, 276, 366, 492, 1077
TscAI CASTG 3 cut(s) 487, 573, 673
TseI GCWGC 1 cut(s) 553
TspDTI ATGAA 2 cut(s) 335, 1095
TspRI CASTG 3 cut(s) 487, 573, 673
Van91I CCANNNNNTGG 1 cut(s) 1004
VneI GTGCAC 1 cut(s) 150
VpaK11BI GGWCC 2 cut(s) 842, 973
XapI RAATTY 3 cut(s) 174, 342, 655
XceI RCATGY 2 cut(s) 436, 1025
XspI CTAG 3 cut(s) 540, 920, 1116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.