Rh7BG170000

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001519
Physical Location & Seq
Forward (+)
13234475 .. 13241881
7407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG170000.1

Sequence Viewer

Length: 1569 bp
ATGGAGGGCCTTGAAGGCGGTTATGTGGGTTTACTGTATGATGAGGTTTCCCTGCAGAATGTGTTAGACATGATAGCTGATTGGACTCCTGAAGAAAGACAGATGCTGAGAGATAAGGTTCCGAAAATGGGTCTAAAGACACCATTTCGTGATGGATTACTCAAGCAAGTTGCCGAAGATGTAGTAAAGTTTGCAAAGGATGGCCTGGAAAGAAGAGGCTTTAAGGAATCAGGGTTCTTAAATGAAGCGGTGGAGATTATGAGAACAGGACAGACACGAGGACGTAAAAGTGCGATAGTTGCTGCAAGTCCTATCACAGAAGATGCTGTAGTAGCTACAAAGCCGCTGATGAAAGAAGATCTTGTAGGCTATCTTGCCTCTGGATGCAAGCCCAAAGAAAAATGGAGAATTGGCATGGAACACGAGCGGTTTGGCTTCGAACTAAATACTTTACGTCCGCTGCAATATGAACAAATAGCTGAGCTGCTCTATGGGATTGCTGGGAGATTTGAGTGGGAGAAAATCATGGAAGGTGGTAAGATTATTGGACTCAAACAGGGTAAGCAGAGCATATCACTAGAGCCAGGAGGTCAATTTGAGCTTAGTGGTGCACCTCTTGAAACTCTGCAACAAATTTGTATTGAAGTCAATTCACAACTCTGTCAGATCAAAGCAGTTGCAGAGAAAATGGGAATCGGATTTCTTGAAATTGGTGGTCAGCCGAAATGGAGAATTCAAGACATACCTATGATGCCCAAGGGAAGATTTGAGATTATTAGAAATTACATGCCAAAAGTTGGCTCGCTTGGACATGATTTGATGTTAAGAACTTGCACTGTACAGGTCAATCTGGACTTCAGTTCTGAAGCAGACATGAAACGGAAATTGCGTGCTAGTCTTGCTTTGCAGCCTATAGCAACAGCCCTATTTGCAAATTCACCTTTCACTGAAGGAAAACCGAATGGTTTTCTAAGCAGGAGAAGTCACATCTACACTGATACTGACAAGGATCGCACTGGCATGCTTCCTTTTGCTTTTGATGACTCCTTTGGGTTTGAGCGGTATGTTGATTATGCTCTTGATGTTCCCATGTACTGTGTTTATCGGAAAAATAAGTGTATCGACTGTACTGGAATGACCTTTCGGGACTTTATCGCAGGAAAGCTTCGTTGCCTTCCAGGTGAATTACCAACACTGAATGATTGGGAGAATCATTTGGGAACTATTTATCCAGAGGTGGGTCTACTATATGATGAAGTTTCCCTGCAGAATGTGTTAGACATGATTGCTGATTGGACTCCTGAAGAAAGACAGATGCTGAGAGATAAGATTCCAAAAACGGGTCTAAAGACACCATTTCGTGAAGGAATACTCAAGCATGTTGCCGAAGATGTAATAAAGTTTGCAAAGGATGGCCTGGAAAGAAGAGGCTTTAAGGAATCAGGGTTTTTAAATGAGGTGGTGGAGGTTGTGAGAACTGGTGTAACACCAGCAGAGAAGCTATTGGAATTATATAATGGGAAATGGAAACAACAGATAGACCCAGTTTTTGAAGAGCTACTATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

522

Amino Acids

59.18

Weight (kDa)

5.64

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 136 - 413 1.1e-69 Glutamate-cysteine ligase family 2(GCS2)
GCS2 PF04107 414 - 519 2.8e-06 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 797
AccBSI CCGCTC 2 cut(s) 427, 1060
AccI GTMKAC 1 cut(s) 1243
AciI CCGC 6 cut(s) 18, 248, 344, 427, 458, 1060
AclWI GGATC 1 cut(s) 1017
AcsI RAATTY 3 cut(s) 633, 732, 934
AcuI CTGAAG 5 cut(s) 111, 841, 885, 969, 1323
AfaI GTAC 3 cut(s) 840, 1094, 1129
AfiI CCNNNNNNNGG 4 cut(s) 128, 797, 1238, 1340
AgsI TTSAA 6 cut(s) 14, 620, 644, 707, 737, 1553
AjnI CCWGG 4 cut(s) 204, 583, 1177, 1416
AloI GAACNNNNNNTCC 4 cut(s) 218, 250, 1213, 1245
AluBI AGCT 8 cut(s) 77, 335, 479, 484, 601, 1165, 1501, 1558
AluI AGCT 8 cut(s) 77, 335, 479, 484, 601, 1165, 1501, 1558
Alw21I GWGCWC 1 cut(s) 613
Alw44I GTGCAC 1 cut(s) 609
AlwI GGATC 1 cut(s) 1017
AlwNI CAGNNNCTG 2 cut(s) 106, 1318
AoxI GGCC 3 cut(s) 7, 202, 1414
ApaLI GTGCAC 1 cut(s) 609
ApeKI GCWGC 4 cut(s) 302, 460, 484, 907
ApoI RAATTY 3 cut(s) 633, 732, 934
ArsI GACNNNNNNTTYG 2 cut(s) 1532, 1564
AspS9I GGNCC 1 cut(s) 7
AsuHPI GGTGA 2 cut(s) 930, 1193
AsuII TTCGAA 1 cut(s) 438
BaeGI GKGCMC 1 cut(s) 613
BauI CACGAG 2 cut(s) 276, 422
Bbv12I GWGCWC 1 cut(s) 613
BbvI GCAGC 4 cut(s) 289, 447, 471, 919
BccI CCATC 3 cut(s) 146, 194, 1406
BciT130I CCWGG 4 cut(s) 206, 585, 1179, 1418
BfaI CTAG 2 cut(s) 578, 894
BfmI CTRYAG 4 cut(s) 53, 327, 912, 1265
BglI GCCNNNNNGGC 1 cut(s) 15
BglII AGATCT 1 cut(s) 358
BisI GCNGC 5 cut(s) 303, 344, 461, 485, 908
BlpI GCTNAGC 1 cut(s) 480
BlsI GCNGC 5 cut(s) 304, 345, 462, 486, 909
Bme1390I CCNGG 4 cut(s) 206, 585, 1179, 1418
BmgT120I GGNCC 1 cut(s) 7
BmiI GGNNCC 1 cut(s) 120
BmrFI CCNGG 4 cut(s) 206, 585, 1179, 1418
BmrI ACTGGG 1 cut(s) 1538
BmsI GCATC 5 cut(s) 93, 313, 374, 741, 1305
BmuI ACTGGG 1 cut(s) 1538
Bpu1102I GCTNAGC 1 cut(s) 480
Bpu14I TTCGAA 1 cut(s) 438
BpuEI CTTGAG 2 cut(s) 146, 1358
BsaJI CCNNGG 1 cut(s) 756
Bsc4I CCNNNNNNNGG 4 cut(s) 128, 797, 1238, 1340
Bse1I ACTGG 4 cut(s) 1021, 1135, 1483, 1544
BseBI CCWGG 4 cut(s) 206, 585, 1179, 1418
BseDI CCNNGG 1 cut(s) 756
BseGI GGATG 3 cut(s) 205, 389, 1417
BseLI CCNNNNNNNGG 4 cut(s) 128, 797, 1238, 1340
BseMII CTCAG 3 cut(s) 98, 471, 1310
BseNI ACTGG 4 cut(s) 1021, 1135, 1483, 1544
BseSI GKGCMC 1 cut(s) 613
BseXI GCAGC 4 cut(s) 289, 447, 471, 919
BseYI CCCAGC 1 cut(s) 500
BshFI GGCC 3 cut(s) 9, 204, 1416
BsiHKAI GWGCWC 1 cut(s) 613
BslFI GGGAC 1 cut(s) 1160
BslI CCNNNNNNNGG 4 cut(s) 128, 797, 1238, 1340
BsmFI GGGAC 1 cut(s) 1160
BsnI GGCC 3 cut(s) 9, 204, 1416
Bsp119I TTCGAA 1 cut(s) 438
Bsp1286I GDGCHC 1 cut(s) 613
Bsp1407I TGTACA 1 cut(s) 838
Bsp143I GATC 3 cut(s) 358, 666, 1009
Bsp1720I GCTNAGC 1 cut(s) 480
BspACI CCGC 6 cut(s) 18, 248, 344, 427, 458, 1060
BspANI GGCC 3 cut(s) 9, 204, 1416
BspCNI CTCAG 3 cut(s) 99, 472, 1311
BspLI GGNNCC 1 cut(s) 120
BspMAI CTGCAG 2 cut(s) 57, 1269
BspPI GGATC 1 cut(s) 1017
BspQI GCTCTTC 1 cut(s) 1548
BspT104I TTCGAA 1 cut(s) 438
BsrBI CCGCTC 2 cut(s) 427, 1060
BsrGI TGTACA 1 cut(s) 838
BsrI ACTGG 4 cut(s) 1021, 1135, 1483, 1544
BssECI CCNNGG 1 cut(s) 756
BssMI GATC 3 cut(s) 358, 666, 1009
BssSI CACGAG 2 cut(s) 276, 422
BssT1I CCWWGG 1 cut(s) 756
Bst2BI CACGAG 2 cut(s) 276, 422
Bst2UI CCWGG 4 cut(s) 206, 585, 1179, 1418
Bst4CI ACNGT 4 cut(s) 36, 838, 1097, 1127
Bst6I CTCTTC 3 cut(s) 208, 1420, 1548
BstAUI TGTACA 1 cut(s) 838
BstBI TTCGAA 1 cut(s) 438
BstC8I GCNNGC 4 cut(s) 389, 803, 891, 1022
BstDEI CTNAG 5 cut(s) 107, 480, 602, 971, 1319
BstF5I GGATG 3 cut(s) 205, 389, 1417
BstKTI GATC 3 cut(s) 361, 669, 1012
BstMBI GATC 3 cut(s) 358, 666, 1009
BstMWI GCNNNNNNNGC 5 cut(s) 15, 299, 332, 899, 929
BstNI CCWGG 4 cut(s) 206, 585, 1179, 1418
BstNSI RCATGY 3 cut(s) 790, 1024, 1382
BstSCI CCNGG 4 cut(s) 204, 583, 1177, 1416
BstSFI CTRYAG 4 cut(s) 53, 327, 912, 1265
BstSLI GKGCMC 1 cut(s) 613
BstV1I GCAGC 4 cut(s) 289, 447, 471, 919
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BsuRI GGCC 3 cut(s) 9, 204, 1416
BtsCI GGATG 3 cut(s) 205, 389, 1417
BtsIMutI CAGTG 5 cut(s) 834, 945, 993, 1014, 1193
Cac8I GCNNGC 4 cut(s) 389, 803, 891, 1022
CaiI CAGNNNCTG 2 cut(s) 106, 1318
Cfr13I GGNCC 1 cut(s) 7
Csp6I GTAC 3 cut(s) 839, 1093, 1128
CviQI GTAC 3 cut(s) 839, 1093, 1128
DdeI CTNAG 5 cut(s) 107, 480, 602, 971, 1319
DpnI GATC 3 cut(s) 360, 668, 1011
DpnII GATC 3 cut(s) 358, 666, 1009
DraI TTTAAA 1 cut(s) 1452
Eam1104I CTCTTC 3 cut(s) 208, 1420, 1548
EarI CTCTTC 3 cut(s) 208, 1420, 1548
Eco130I CCWWGG 1 cut(s) 756
Eco57I CTGAAG 5 cut(s) 111, 841, 885, 969, 1323
EcoO109I RGGNCCY 1 cut(s) 7
EcoRI GAATTC 1 cut(s) 732
EcoRII CCWGG 4 cut(s) 204, 583, 1177, 1416
EcoT14I CCWWGG 1 cut(s) 756
ErhI CCWWGG 1 cut(s) 756
FalI AAGNNNNNCTT 2 cut(s) 345, 377
FaqI GGGAC 1 cut(s) 1160
FblI GTMKAC 1 cut(s) 1243
Fnu4HI GCNGC 5 cut(s) 303, 344, 461, 485, 908
FokI GGATG 3 cut(s) 212, 396, 1424
Fsp4HI GCNGC 5 cut(s) 303, 344, 461, 485, 908
FspBI CTAG 2 cut(s) 578, 894
GluI GCNGC 5 cut(s) 303, 344, 461, 485, 908
GsaI CCCAGC 1 cut(s) 504
HaeIII GGCC 3 cut(s) 9, 204, 1416
HindIII AAGCTT 1 cut(s) 1163
HinfI GANTC 9 cut(s) 85, 227, 549, 693, 1043, 1210, 1297, 1330, 1439
HphI GGTGA 2 cut(s) 930, 1193
Hpy166II GTNNAC 3 cut(s) 32, 611, 1244
Hpy188I TCNGA 5 cut(s) 123, 666, 698, 865, 1107
Hpy8I GTNNAC 3 cut(s) 32, 611, 1244
HpyAV CCTTC 5 cut(s) 8, 524, 944, 1184, 1358
HpyCH4III ACNGT 4 cut(s) 36, 838, 1097, 1127
HpyCH4IV ACGT 2 cut(s) 283, 454
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 299, 332, 899, 929
HpyF3I CTNAG 5 cut(s) 107, 480, 602, 971, 1319
HpySE526I ACGT 2 cut(s) 283, 454
Kzo9I GATC 3 cut(s) 358, 666, 1009
LguI GCTCTTC 1 cut(s) 1548
Lsp1109I GCAGC 4 cut(s) 289, 447, 471, 919
LweI GCATC 5 cut(s) 93, 313, 374, 741, 1305
MaeI CTAG 2 cut(s) 578, 894
MaeII ACGT 2 cut(s) 283, 454
MaeIII GTNAC 2 cut(s) 983, 1483
MalI GATC 3 cut(s) 360, 668, 1011
MbiI CCGCTC 2 cut(s) 427, 1060
MboI GATC 3 cut(s) 358, 666, 1009
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 1 cut(s) 613
MlyI GAGTC 4 cut(s) 79, 543, 1037, 1291
MseI TTAA 5 cut(s) 222, 239, 824, 1434, 1451
MslI CAYNNNNRTG 2 cut(s) 746, 1019
MspA1I CMGCKG 2 cut(s) 346, 460
MspR9I CCNGG 4 cut(s) 206, 585, 1179, 1418
MvaI CCWGG 4 cut(s) 206, 585, 1179, 1418
MwoI GCNNNNNNNGC 5 cut(s) 15, 299, 332, 899, 929
NdeII GATC 3 cut(s) 358, 666, 1009
NlaIV GGNNCC 1 cut(s) 120
NmuCI GTSAC 1 cut(s) 983
NspI RCATGY 3 cut(s) 790, 1024, 1382
NspV TTCGAA 1 cut(s) 438
PaeI GCATGC 1 cut(s) 1024
PciSI GCTCTTC 1 cut(s) 1548
PcsI WCGNNNNNNNCGW 1 cut(s) 886
PfeI GAWTC 5 cut(s) 227, 693, 1210, 1330, 1439
PflMI CCANNNNNTGG 1 cut(s) 797
PkrI GCNGC 5 cut(s) 304, 345, 462, 486, 909
PleI GAGTC 4 cut(s) 79, 543, 1037, 1291
PpsI GAGTC 4 cut(s) 79, 543, 1037, 1291
Psp6I CCWGG 4 cut(s) 204, 583, 1177, 1416
PspFI CCCAGC 1 cut(s) 500
PspGI CCWGG 4 cut(s) 204, 583, 1177, 1416
PspN4I GGNNCC 1 cut(s) 120
PspPI GGNCC 1 cut(s) 7
PstI CTGCAG 2 cut(s) 57, 1269
PstNI CAGNNNCTG 2 cut(s) 106, 1318
PsuI RGATCY 1 cut(s) 358
RsaI GTAC 3 cut(s) 840, 1094, 1129
RsaNI GTAC 3 cut(s) 839, 1093, 1128
RseI CAYNNNNRTG 2 cut(s) 746, 1019
SapI GCTCTTC 1 cut(s) 1548
SaqAI TTAA 5 cut(s) 222, 239, 824, 1434, 1451
SatI GCNGC 5 cut(s) 303, 344, 461, 485, 908
Sau3AI GATC 3 cut(s) 358, 666, 1009
Sau96I GGNCC 1 cut(s) 7
SchI GAGTC 4 cut(s) 79, 543, 1037, 1291
ScrFI CCNGG 4 cut(s) 206, 585, 1179, 1418
SduI GDGCHC 1 cut(s) 613
SfaNI GCATC 5 cut(s) 93, 313, 374, 741, 1305
SfcI CTRYAG 4 cut(s) 53, 327, 912, 1265
SfuI TTCGAA 1 cut(s) 438
SmiMI CAYNNNNRTG 2 cut(s) 746, 1019
SmlI CTYRAG 2 cut(s) 161, 1373
SmoI CTYRAG 2 cut(s) 161, 1373
SphI GCATGC 1 cut(s) 1024
SsiI CCGC 6 cut(s) 18, 248, 344, 427, 458, 1060
SspMI CTAG 2 cut(s) 578, 894
StyD4I CCNGG 4 cut(s) 204, 583, 1177, 1416
StyI CCWWGG 1 cut(s) 756
TaaI ACNGT 4 cut(s) 36, 838, 1097, 1127
TaiI ACGT 2 cut(s) 286, 457
TaqI TCGA 2 cut(s) 438, 1122
TatI WGTACW 3 cut(s) 838, 1092, 1127
TauI GCSGC 1 cut(s) 346
TfiI GAWTC 5 cut(s) 227, 693, 1210, 1330, 1439
Tru1I TTAA 5 cut(s) 222, 239, 824, 1434, 1451
Tru9I TTAA 5 cut(s) 222, 239, 824, 1434, 1451
TscAI CASTG 5 cut(s) 841, 952, 1000, 1021, 1200
TseFI GTSAC 1 cut(s) 983
TseI GCWGC 4 cut(s) 302, 460, 484, 907
Tsp45I GTSAC 1 cut(s) 983
TspDTI ATGAA 5 cut(s) 258, 365, 483, 890, 1269
TspGWI ACGGA 1 cut(s) 895
TspRI CASTG 5 cut(s) 841, 952, 1000, 1021, 1200
Van91I CCANNNNNTGG 1 cut(s) 797
VneI GTGCAC 1 cut(s) 609
XapI RAATTY 3 cut(s) 633, 732, 934
XceI RCATGY 3 cut(s) 790, 1024, 1382
XmiI GTMKAC 1 cut(s) 1243
XspI CTAG 2 cut(s) 578, 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.