pycom14g10010

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001519
Physical Location & Seq
Reverse (-)
12380982 .. 12386388
5407 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g10010.4

Sequence Viewer

Length: 1125 bp
ATGGAAGGTGACAAAATTATTGGCCTCAAACAGGGTAAGCAAAGCATATCACTGGAGCCTGGAGGTCAATTTGAACTTAGTGGTGCACCTCTTGAGACTCTGCATCAAACTTGTGCCGAAGTCAATTCACACCTTTATCAGGTCAAAGCACTTTCAGAGGAAATGGGAATTGGGTTTCTTGGAATTGGTTTTCAGCCTAAATGGGGACTTAAGGACATACCTATAATGCCCAAGGGAAGATATGATATTATGAGAAATTACATGCCAAAAGTTGGCACTCTTGGACTGGATATGATGTTCAGAACTTGCACTGTACAGGTTAATCTGGACTTCAGTTCTGAAGCAGACATGATAAGGAAATTTCGTGCTGGTCTTGTTTTGCAGCCTATAGCAACAGCTCTATTCGCAAATTCACCTTTTACTGAAGGAAAGCCAAATGGTTTTCTAAGCATGAGAAGCCAAATTTGGACCGATACGGATAATAATCGTGCTGGCATGCTTCCTTTTGTTTTTGATGACTCTTTTGGGTTTGAGCAGTATGTTGATTATGCTCTCGATGTTCCCATGTACTTTGTTTATCGGCAAAAGAAGTATATCGATTGCACTGGAATGAGCTTCCGGGACTTTTTGGCAGGGAAGCTTCCTTGCATTCCGGGTGAATTGCCAACCCTGAACGATTGGGAGAATCATTTGACAACCATATTCCCTGAGGTCAGGCTAAAGAGATACTTGGAGATGAGGGGTGCTGATGGAGGGCCTTGGAGGAGATTATGTGCTTTGCCCGCCTTTTGGGTAGGTATATTGTATGATGAGGTTTCCCTACAAAACGTATTAGACCTTACAGCTGATTGGACTCCTGAAGAAAGACAGTTGCTGAGAAATAAGGTCCCCTTAACTGGTCTAAAGACACCATTTAGGGATGGATTGCTCAAGCATGTAGCTCAAGATGTTGTAAAGTTGGCAAAGGATGGCCTGGAAAGGAGAGGTTTCAAAGAAACAGGATTCTTGAATGAGGTGGTGGAGTTGGTTAGAGCTGGTGTAACACCAGCTGAGAAGCTATTGGAGTTGTACAATGGGAAATGGGGACAACAGATAGACCCCGTTTTTGAAGAGCTACTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

375

Amino Acids

42.41

Weight (kDa)

5.48

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 272
AciI CCGC 1 cut(s) 783
AcsI RAATTY 3 cut(s) 359, 409, 462
AcuI CTGAAG 4 cut(s) 316, 360, 444, 879
AfaI GTAC 3 cut(s) 315, 569, 1070
AfiI CCNNNNNNNGG 6 cut(s) 31, 139, 203, 272, 789, 896
AflII CTTAAG 1 cut(s) 209
AgsI TTSAA 4 cut(s) 74, 991, 1009, 1109
AjnI CCWGG 2 cut(s) 58, 972
AjuI GAANNNNNNNTTGG 4 cut(s) 153, 185, 448, 480
AloI GAACNNNNNNTCC 2 cut(s) 281, 313
AluBI AGCT 9 cut(s) 398, 615, 640, 845, 941, 1034, 1049, 1057, 1114
AluI AGCT 9 cut(s) 398, 615, 640, 845, 941, 1034, 1049, 1057, 1114
Alw21I GWGCWC 1 cut(s) 88
Alw26I GTCTC 1 cut(s) 89
Alw44I GTGCAC 1 cut(s) 84
AlwNI CAGNNNCTG 1 cut(s) 874
AoxI GGCC 3 cut(s) 22, 755, 970
ApaLI GTGCAC 1 cut(s) 84
ApeKI GCWGC 1 cut(s) 382
ApoI RAATTY 3 cut(s) 359, 409, 462
ArsI GACNNNNNNTTYG 2 cut(s) 1088, 1120
AspS9I GGNCC 3 cut(s) 468, 755, 886
AsuC2I CCSGG 2 cut(s) 620, 654
AsuHPI GGTGA 3 cut(s) 20, 405, 668
AvaII GGWCC 2 cut(s) 468, 886
AxyI CCTNAGG 1 cut(s) 708
BaeGI GKGCMC 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 88
BbvI GCAGC 1 cut(s) 394
BccI CCATC 3 cut(s) 743, 914, 962
BciT130I CCWGG 2 cut(s) 60, 974
BcnI CCSGG 2 cut(s) 620, 654
BcoDI GTCTC 1 cut(s) 89
BfmI CTRYAG 1 cut(s) 387
BfrI CTTAAG 1 cut(s) 209
BisI GCNGC 1 cut(s) 383
BlsI GCNGC 1 cut(s) 384
Bme1390I CCNGG 4 cut(s) 60, 620, 654, 974
Bme18I GGWCC 2 cut(s) 468, 886
BmgT120I GGNCC 3 cut(s) 468, 755, 886
BmiI GGNNCC 2 cut(s) 57, 888
BmrFI CCNGG 4 cut(s) 60, 620, 654, 974
BmsI GCATC 1 cut(s) 112
BpmI CTGGAG 2 cut(s) 74, 81
BpuEI CTTGAG 3 cut(s) 113, 914, 927
BpuMI CCSGG 2 cut(s) 620, 654
Bsa29I ATCGAT 1 cut(s) 597
BsaBI GATNNNNATC 1 cut(s) 483
BsaJI CCNNGG 2 cut(s) 231, 758
BsaXI ACNNNNNCTCC 2 cut(s) 757, 787
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 139, 203, 272, 789, 896
Bse1I ACTGG 4 cut(s) 57, 291, 610, 901
Bse21I CCTNAGG 1 cut(s) 708
Bse8I GATNNNNATC 1 cut(s) 483
BseBI CCWGG 2 cut(s) 60, 974
BseCI ATCGAT 1 cut(s) 597
BseDI CCNNGG 2 cut(s) 231, 758
BseGI GGATG 2 cut(s) 925, 973
BseJI GATNNNNATC 1 cut(s) 483
BseLI CCNNNNNNNGG 6 cut(s) 31, 139, 203, 272, 789, 896
BseMII CTCAG 3 cut(s) 699, 866, 1041
BseNI ACTGG 4 cut(s) 57, 291, 610, 901
BseRI GAGGAG 1 cut(s) 778
BseSI GKGCMC 1 cut(s) 88
BseXI GCAGC 1 cut(s) 394
BshFI GGCC 3 cut(s) 24, 757, 972
BshVI ATCGAT 1 cut(s) 597
BsiHKAI GWGCWC 1 cut(s) 88
BsiSI CCGG 2 cut(s) 619, 653
BslFI GGGAC 4 cut(s) 219, 635, 872, 1098
BslI CCNNNNNNNGG 6 cut(s) 31, 139, 203, 272, 789, 896
BsmAI GTCTC 1 cut(s) 89
BsmFI GGGAC 4 cut(s) 219, 635, 872, 1098
BsmI GAATGC 1 cut(s) 648
BsnI GGCC 3 cut(s) 24, 757, 972
Bsp1286I GDGCHC 1 cut(s) 88
Bsp1407I TGTACA 2 cut(s) 313, 1068
BspACI CCGC 1 cut(s) 783
BspANI GGCC 3 cut(s) 24, 757, 972
BspCNI CTCAG 3 cut(s) 700, 867, 1042
BspDI ATCGAT 1 cut(s) 597
BspLI GGNNCC 2 cut(s) 57, 888
BspQI GCTCTTC 1 cut(s) 1104
BspTI CTTAAG 1 cut(s) 209
BsrGI TGTACA 2 cut(s) 313, 1068
BsrI ACTGG 4 cut(s) 57, 291, 610, 901
BssECI CCNNGG 2 cut(s) 231, 758
BssT1I CCWWGG 2 cut(s) 231, 758
Bst2UI CCWGG 2 cut(s) 60, 974
Bst4CI ACNGT 2 cut(s) 313, 870
Bst6I CTCTTC 1 cut(s) 1104
BstAFI CTTAAG 1 cut(s) 209
BstAUI TGTACA 2 cut(s) 313, 1068
BstC8I GCNNGC 3 cut(s) 493, 497, 783
BstDEI CTNAG 5 cut(s) 77, 446, 708, 875, 1050
BstENI CCTNNNNNAGG 2 cut(s) 29, 137
BstF5I GGATG 2 cut(s) 925, 973
BstMAI GTCTC 1 cut(s) 89
BstMWI GCNNNNNNNGC 3 cut(s) 404, 456, 782
BstNI CCWGG 2 cut(s) 60, 974
BstNSI RCATGY 3 cut(s) 265, 499, 938
BstSCI CCNGG 4 cut(s) 58, 618, 652, 972
BstSFI CTRYAG 1 cut(s) 387
BstSLI GKGCMC 1 cut(s) 88
BstV1I GCAGC 1 cut(s) 394
Bsu15I ATCGAT 1 cut(s) 597
Bsu36I CCTNAGG 1 cut(s) 708
BsuRI GGCC 3 cut(s) 24, 757, 972
BsuTUI ATCGAT 1 cut(s) 597
BtsCI GGATG 2 cut(s) 925, 973
BtsIMutI CAGTG 3 cut(s) 50, 309, 603
Cac8I GCNNGC 3 cut(s) 493, 497, 783
CaiI CAGNNNCTG 1 cut(s) 874
Cfr13I GGNCC 3 cut(s) 468, 755, 886
ClaI ATCGAT 1 cut(s) 597
Csp6I GTAC 3 cut(s) 314, 568, 1069
CviAII CATG 6 cut(s) 262, 349, 451, 496, 565, 935
CviQI GTAC 3 cut(s) 314, 568, 1069
DdeI CTNAG 5 cut(s) 77, 446, 708, 875, 1050
Eam1104I CTCTTC 1 cut(s) 1104
EarI CTCTTC 1 cut(s) 1104
Eco130I CCWWGG 2 cut(s) 231, 758
Eco47I GGWCC 2 cut(s) 468, 886
Eco57I CTGAAG 4 cut(s) 316, 360, 444, 879
Eco81I CCTNAGG 1 cut(s) 708
EcoNI CCTNNNNNAGG 2 cut(s) 29, 137
EcoO109I RGGNCCY 2 cut(s) 755, 886
EcoRII CCWGG 2 cut(s) 58, 972
EcoT14I CCWWGG 2 cut(s) 231, 758
ErhI CCWWGG 2 cut(s) 231, 758
FaeI CATG 6 cut(s) 265, 352, 454, 499, 568, 938
FalI AAGNNNNNCTT 4 cut(s) 713, 745, 875, 907
FaqI GGGAC 4 cut(s) 219, 635, 872, 1098
FatI CATG 6 cut(s) 261, 348, 450, 495, 564, 934
FauI CCCGC 1 cut(s) 790
Fnu4HI GCNGC 1 cut(s) 383
FokI GGATG 2 cut(s) 932, 980
Fsp4HI GCNGC 1 cut(s) 383
GluI GCNGC 1 cut(s) 383
GsuI CTGGAG 2 cut(s) 74, 81
HaeIII GGCC 3 cut(s) 24, 757, 972
HapII CCGG 2 cut(s) 619, 653
Hin1II CATG 6 cut(s) 265, 352, 454, 499, 568, 938
HindIII AAGCTT 1 cut(s) 638
HinfI GANTC 5 cut(s) 97, 518, 685, 853, 1002
HpaII CCGG 2 cut(s) 619, 653
HphI GGTGA 3 cut(s) 20, 405, 668
Hpy166II GTNNAC 1 cut(s) 86
Hpy188I TCNGA 3 cut(s) 157, 302, 340
Hpy188III TCNNGA 6 cut(s) 92, 326, 554, 857, 944, 1006
Hpy8I GTNNAC 1 cut(s) 86
HpyAV CCTTC 1 cut(s) 419
HpyCH4III ACNGT 2 cut(s) 313, 870
HpyCH4IV ACGT 1 cut(s) 828
HpyCH4V TGCA 6 cut(s) 86, 103, 309, 382, 603, 648
HpyF10VI GCNNNNNNNGC 3 cut(s) 404, 456, 782
HpyF3I CTNAG 5 cut(s) 77, 446, 708, 875, 1050
HpySE526I ACGT 1 cut(s) 828
Hsp92II CATG 6 cut(s) 265, 352, 454, 499, 568, 938
LguI GCTCTTC 1 cut(s) 1104
LmnI GCTCC 1 cut(s) 55
Lsp1109I GCAGC 1 cut(s) 394
LweI GCATC 1 cut(s) 112
MaeII ACGT 1 cut(s) 828
MaeIII GTNAC 2 cut(s) 8, 1039
MboII GAAGA 3 cut(s) 249, 872, 1121
MhlI GDGCHC 1 cut(s) 88
MlyI GAGTC 3 cut(s) 91, 512, 847
MseI TTAA 3 cut(s) 210, 321, 893
MslI CAYNNNNRTG 1 cut(s) 608
MspA1I CMGCKG 2 cut(s) 845, 1049
MspCI CTTAAG 1 cut(s) 209
MspI CCGG 2 cut(s) 619, 653
MspR9I CCNGG 4 cut(s) 60, 620, 654, 974
Mva1269I GAATGC 1 cut(s) 648
MvaI CCWGG 2 cut(s) 60, 974
MwoI GCNNNNNNNGC 3 cut(s) 404, 456, 782
NciI CCSGG 2 cut(s) 620, 654
NlaIII CATG 6 cut(s) 265, 352, 454, 499, 568, 938
NlaIV GGNNCC 2 cut(s) 57, 888
NmuCI GTSAC 1 cut(s) 8
NspI RCATGY 3 cut(s) 265, 499, 938
PaeI GCATGC 1 cut(s) 499
PciSI GCTCTTC 1 cut(s) 1104
PctI GAATGC 1 cut(s) 648
PfeI GAWTC 2 cut(s) 685, 1002
PflMI CCANNNNNTGG 1 cut(s) 272
PfoI TCCNGGA 1 cut(s) 618
PkrI GCNGC 1 cut(s) 384
PleI GAGTC 3 cut(s) 91, 512, 847
PpsI GAGTC 3 cut(s) 91, 512, 847
PpuMI RGGWCCY 1 cut(s) 886
Psp5II RGGWCCY 1 cut(s) 886
Psp6I CCWGG 2 cut(s) 58, 972
PspGI CCWGG 2 cut(s) 58, 972
PspN4I GGNNCC 2 cut(s) 57, 888
PspPI GGNCC 3 cut(s) 468, 755, 886
PspPPI RGGWCCY 1 cut(s) 886
PstNI CAGNNNCTG 1 cut(s) 874
PvuII CAGCTG 2 cut(s) 845, 1049
RsaI GTAC 3 cut(s) 315, 569, 1070
RsaNI GTAC 3 cut(s) 314, 568, 1069
RseI CAYNNNNRTG 1 cut(s) 608
SapI GCTCTTC 1 cut(s) 1104
SaqAI TTAA 3 cut(s) 210, 321, 893
SatI GCNGC 1 cut(s) 383
Sau96I GGNCC 3 cut(s) 468, 755, 886
SchI GAGTC 3 cut(s) 91, 512, 847
ScrFI CCNGG 4 cut(s) 60, 620, 654, 974
SduI GDGCHC 1 cut(s) 88
SfaNI GCATC 1 cut(s) 112
SfcI CTRYAG 1 cut(s) 387
SinI GGWCC 2 cut(s) 468, 886
SmiMI CAYNNNNRTG 1 cut(s) 608
SmlI CTYRAG 4 cut(s) 92, 209, 929, 942
SmoI CTYRAG 4 cut(s) 92, 209, 929, 942
SphI GCATGC 1 cut(s) 499
SsiI CCGC 1 cut(s) 783
StyD4I CCNGG 4 cut(s) 58, 618, 652, 972
StyI CCWWGG 2 cut(s) 231, 758
TaaI ACNGT 2 cut(s) 313, 870
TaiI ACGT 1 cut(s) 831
TaqI TCGA 2 cut(s) 555, 597
TaqII GACCGA 1 cut(s) 485
TatI WGTACW 3 cut(s) 313, 567, 1068
TfiI GAWTC 2 cut(s) 685, 1002
Tru1I TTAA 3 cut(s) 210, 321, 893
Tru9I TTAA 3 cut(s) 210, 321, 893
TscAI CASTG 3 cut(s) 57, 316, 610
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 1 cut(s) 382
Tsp45I GTSAC 1 cut(s) 8
TspGWI ACGGA 1 cut(s) 491
TspRI CASTG 3 cut(s) 57, 316, 610
Van91I CCANNNNNTGG 1 cut(s) 272
Vha464I CTTAAG 1 cut(s) 209
VneI GTGCAC 1 cut(s) 84
VpaK11BI GGWCC 2 cut(s) 468, 886
XagI CCTNNNNNAGG 2 cut(s) 29, 137
XapI RAATTY 3 cut(s) 359, 409, 462
XceI RCATGY 3 cut(s) 265, 499, 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.