pycom06g07940

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001519
Physical Location & Seq
Reverse (-)
12941043 .. 12945404
4362 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g07940.1

Sequence Viewer

Length: 1137 bp
ATGGAAGGCGACAAAATTATTGGACTCAAACAGGGTAAGCAAAGCATATCACTGGAGCCTGGAGGTCAATTTGAACTTAGTGGCGCACCTCTTGCGACACTGCATCAAACTTGTGCTGAAGTCAATTCGCACCTTTATCAGGTCAAAGCAATTACAGAGGAAATGGGAATTGGATTTCTTGGAATCGGTTTTCAGCCCAAATGGGGACTTAAGGACATACCTATTATGCCCAAGGGAAGATATGATATTATGAGAAATTACATGCCAAAAGTTGGCACCCTTGGACTTGATATGATGTTCAGAACTTGCACTGTACAGGTTAATCTGGACTTCAGTTCTGAGGCAGACATGATAAGGAAATTTCGTGCTGGTCTTGCATTGCAGCCTATAGCAACAGCTCTATTCGCAAATTCACCTTTCACTGAAGGAAAGCCAAATGGTTTTCTAAGCATGAGAAGCCAAATTTGGACTGATACGGACAATAATCGTGCTGGCATGCTTCCTTTTGTGTTCGATGACTCTTTTGGGTTTGAGCAGTATGTAGATTATGCTCTTGATGTTCCCATGTACTTTGTTTATCGGAACAAGAAGTATATCGATTGCACTGGAATGACCTTCCGGGTTTCTATCTTTCCTTTACGGTCTTGGAAGCTTCCTTGCATTCCGGGTGAATTGCCAACCCTGAACGATTGGGAGAATCATTTGACAACTATATTCCCTGAGGTCAGGCTAAAAAGATACTTGGAGATGAGGGGTGCTGATGGAGGGCCTTGGAGGAGATTATGTGCTTTGCCCGCATTTTGGGTAGGTATTTTGTACGACGAGGTTTCCTTACAAAATGTGTTAGACCTTACAGCTGATTGGACCCCTGAAGAAAGACAGATGCTGAGAAATAAGGTCCCCATAACTGGTCTAAAGACGCCATTTCGGGATGGATTGCTCAAGCATGTAGCTCAAGATGTTGTAAAGTTGGCAAGGGATGGCCTGGAAAGACGAGGCTTCAAAGAAACAGGATTCTTGAATGAGGTGGCGGAGGTGGTTAGAACTGGTGTAACACCAGCCGAGAAGCTTTTGGAATTGTACAATGGGAAATGGGGACAACAGATGGACCCTGTTTTCGAGGAGCTACTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

379

Amino Acids

42.98

Weight (kDa)

5.95

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 11 - 373 9.8e-100 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 275
AccB7I CCANNNNNTGG 1 cut(s) 272
AciI CCGC 2 cut(s) 795, 1031
AcsI RAATTY 3 cut(s) 359, 409, 462
AcuI CTGAAG 4 cut(s) 138, 316, 444, 891
AcyI GRCGYC 1 cut(s) 920
AfaI GTAC 4 cut(s) 315, 569, 818, 1082
AfiI CCNNNNNNNGG 5 cut(s) 139, 203, 272, 801, 908
AflII CTTAAG 1 cut(s) 209
AgsI TTSAA 3 cut(s) 74, 1003, 1021
AjnI CCWGG 2 cut(s) 58, 984
AjuI GAANNNNNNNTTGG 4 cut(s) 153, 185, 448, 480
AluBI AGCT 6 cut(s) 398, 652, 857, 953, 1069, 1126
AluI AGCT 6 cut(s) 398, 652, 857, 953, 1069, 1126
AlwNI CAGNNNCTG 1 cut(s) 886
AoxI GGCC 2 cut(s) 767, 982
ApeKI GCWGC 1 cut(s) 382
ApoI RAATTY 3 cut(s) 359, 409, 462
ArsI GACNNNNNNTTYG 2 cut(s) 1100, 1132
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 4 cut(s) 767, 864, 898, 1108
AsuC2I CCSGG 2 cut(s) 620, 666
AsuHPI GGTGA 2 cut(s) 405, 680
AvaII GGWCC 3 cut(s) 864, 898, 1108
AxyI CCTNAGG 1 cut(s) 720
BanI GGYRCC 1 cut(s) 275
BbvI GCAGC 1 cut(s) 394
BccI CCATC 4 cut(s) 755, 926, 974, 1099
BciT130I CCWGG 2 cut(s) 60, 986
BcnI CCSGG 2 cut(s) 620, 666
BfmI CTRYAG 1 cut(s) 387
BfrI CTTAAG 1 cut(s) 209
BisI GCNGC 1 cut(s) 383
BlsI GCNGC 1 cut(s) 384
Bme1390I CCNGG 4 cut(s) 60, 620, 666, 986
Bme18I GGWCC 3 cut(s) 864, 898, 1108
BmgT120I GGNCC 4 cut(s) 767, 864, 898, 1108
BmiI GGNNCC 5 cut(s) 57, 277, 866, 900, 1110
BmrFI CCNGG 4 cut(s) 60, 620, 666, 986
BmsI GCATC 2 cut(s) 112, 873
BpmI CTGGAG 2 cut(s) 74, 81
BpuEI CTTGAG 2 cut(s) 926, 939
BpuMI CCSGG 2 cut(s) 620, 666
Bsa29I ATCGAT 1 cut(s) 597
BsaHI GRCGYC 1 cut(s) 920
BsaJI CCNNGG 3 cut(s) 231, 280, 770
BsaXI ACNNNNNCTCC 2 cut(s) 769, 799
Bsc4I CCNNNNNNNGG 5 cut(s) 139, 203, 272, 801, 908
Bse1I ACTGG 4 cut(s) 57, 610, 913, 1051
Bse21I CCTNAGG 1 cut(s) 720
Bse3DI GCAATG 1 cut(s) 377
BseBI CCWGG 2 cut(s) 60, 986
BseCI ATCGAT 1 cut(s) 597
BseDI CCNNGG 3 cut(s) 231, 280, 770
BseGI GGATG 2 cut(s) 937, 985
BseLI CCNNNNNNNGG 5 cut(s) 139, 203, 272, 801, 908
BseMI GCAATG 1 cut(s) 377
BseMII CTCAG 3 cut(s) 330, 711, 878
BseNI ACTGG 4 cut(s) 57, 610, 913, 1051
BseRI GAGGAG 2 cut(s) 790, 1136
BseXI GCAGC 1 cut(s) 394
BshFI GGCC 2 cut(s) 769, 984
BshNI GGYRCC 1 cut(s) 275
BshVI ATCGAT 1 cut(s) 597
BsiSI CCGG 2 cut(s) 619, 665
BslFI GGGAC 3 cut(s) 219, 884, 1110
BslI CCNNNNNNNGG 5 cut(s) 139, 203, 272, 801, 908
BsmFI GGGAC 3 cut(s) 219, 884, 1110
BsmI GAATGC 1 cut(s) 660
BsnI GGCC 2 cut(s) 769, 984
Bsp1407I TGTACA 2 cut(s) 313, 1080
BspACI CCGC 2 cut(s) 795, 1031
BspANI GGCC 2 cut(s) 769, 984
BspCNI CTCAG 3 cut(s) 331, 712, 879
BspDI ATCGAT 1 cut(s) 597
BspLI GGNNCC 5 cut(s) 57, 277, 866, 900, 1110
BspT107I GGYRCC 1 cut(s) 275
BspTI CTTAAG 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 377
BsrGI TGTACA 2 cut(s) 313, 1080
BsrI ACTGG 4 cut(s) 57, 610, 913, 1051
BssECI CCNNGG 3 cut(s) 231, 280, 770
BssNI GRCGYC 1 cut(s) 920
BssT1I CCWWGG 3 cut(s) 231, 280, 770
Bst2UI CCWGG 2 cut(s) 60, 986
Bst4CI ACNGT 2 cut(s) 313, 642
BstACI GRCGYC 1 cut(s) 920
BstAFI CTTAAG 1 cut(s) 209
BstAPI GCANNNNNTGC 1 cut(s) 92
BstAUI TGTACA 2 cut(s) 313, 1080
BstC8I GCNNGC 3 cut(s) 493, 497, 795
BstDEI CTNAG 5 cut(s) 77, 339, 446, 720, 887
BstENI CCTNNNNNAGG 1 cut(s) 137
BstF5I GGATG 2 cut(s) 937, 985
BstHHI GCGC 1 cut(s) 86
BstMWI GCNNNNNNNGC 5 cut(s) 92, 374, 404, 456, 794
BstNI CCWGG 2 cut(s) 60, 986
BstNSI RCATGY 3 cut(s) 265, 499, 950
BstSCI CCNGG 4 cut(s) 58, 618, 664, 984
BstSFI CTRYAG 1 cut(s) 387
BstV1I GCAGC 1 cut(s) 394
Bsu15I ATCGAT 1 cut(s) 597
Bsu36I CCTNAGG 1 cut(s) 720
BsuRI GGCC 2 cut(s) 769, 984
BsuTUI ATCGAT 1 cut(s) 597
BtsCI GGATG 2 cut(s) 937, 985
BtsI GCAGTG 1 cut(s) 98
BtsIMutI CAGTG 5 cut(s) 50, 98, 309, 420, 603
Cac8I GCNNGC 3 cut(s) 493, 497, 795
CaiI CAGNNNCTG 1 cut(s) 886
CfoI GCGC 1 cut(s) 86
Cfr13I GGNCC 4 cut(s) 767, 864, 898, 1108
ClaI ATCGAT 1 cut(s) 597
CseI GACGC 1 cut(s) 928
Csp6I GTAC 4 cut(s) 314, 568, 817, 1081
CviAII CATG 6 cut(s) 262, 349, 451, 496, 565, 947
CviQI GTAC 4 cut(s) 314, 568, 817, 1081
DdeI CTNAG 5 cut(s) 77, 339, 446, 720, 887
EciI GGCGGA 1 cut(s) 1046
Eco130I CCWWGG 3 cut(s) 231, 280, 770
Eco47I GGWCC 3 cut(s) 864, 898, 1108
Eco57I CTGAAG 4 cut(s) 138, 316, 444, 891
Eco81I CCTNAGG 1 cut(s) 720
EcoNI CCTNNNNNAGG 1 cut(s) 137
EcoO109I RGGNCCY 2 cut(s) 767, 898
EcoRII CCWGG 2 cut(s) 58, 984
EcoT14I CCWWGG 3 cut(s) 231, 280, 770
ErhI CCWWGG 3 cut(s) 231, 280, 770
FaeI CATG 6 cut(s) 265, 352, 454, 499, 568, 950
FaqI GGGAC 3 cut(s) 219, 884, 1110
FatI CATG 6 cut(s) 261, 348, 450, 495, 564, 946
FauI CCCGC 1 cut(s) 802
Fnu4HI GCNGC 1 cut(s) 383
FokI GGATG 2 cut(s) 944, 992
Fsp4HI GCNGC 1 cut(s) 383
GlaI GCGC 1 cut(s) 85
GluI GCNGC 1 cut(s) 383
GsuI CTGGAG 2 cut(s) 74, 81
HaeIII GGCC 2 cut(s) 769, 984
HapII CCGG 2 cut(s) 619, 665
HgaI GACGC 1 cut(s) 928
HhaI GCGC 1 cut(s) 86
Hin1I GRCGYC 1 cut(s) 920
Hin1II CATG 6 cut(s) 265, 352, 454, 499, 568, 950
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HindIII AAGCTT 2 cut(s) 650, 1067
HinfI GANTC 5 cut(s) 24, 183, 518, 697, 1014
HpaII CCGG 2 cut(s) 619, 665
HphI GGTGA 2 cut(s) 405, 680
Hpy188I TCNGA 3 cut(s) 302, 340, 582
Hpy188III TCNNGA 5 cut(s) 326, 554, 929, 956, 1018
Hpy99I CGWCG 1 cut(s) 824
HpyAV CCTTC 2 cut(s) 419, 625
HpyCH4III ACNGT 2 cut(s) 313, 642
HpyCH4V TGCA 6 cut(s) 103, 309, 377, 382, 603, 660
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 374, 404, 456, 794
HpyF3I CTNAG 5 cut(s) 77, 339, 446, 720, 887
Hsp92I GRCGYC 1 cut(s) 920
Hsp92II CATG 6 cut(s) 265, 352, 454, 499, 568, 950
HspAI GCGC 1 cut(s) 84
LmnI GCTCC 2 cut(s) 55, 1123
Lsp1109I GCAGC 1 cut(s) 394
LweI GCATC 2 cut(s) 112, 873
MaeIII GTNAC 1 cut(s) 1051
MboII GAAGA 2 cut(s) 249, 884
MlyI GAGTC 2 cut(s) 18, 512
MseI TTAA 2 cut(s) 210, 321
MslI CAYNNNNRTG 1 cut(s) 608
MspA1I CMGCKG 1 cut(s) 857
MspCI CTTAAG 1 cut(s) 209
MspI CCGG 2 cut(s) 619, 665
MspR9I CCNGG 4 cut(s) 60, 620, 666, 986
Mva1269I GAATGC 1 cut(s) 660
MvaI CCWGG 2 cut(s) 60, 986
MwoI GCNNNNNNNGC 5 cut(s) 92, 374, 404, 456, 794
NciI CCSGG 2 cut(s) 620, 666
NlaIII CATG 6 cut(s) 265, 352, 454, 499, 568, 950
NlaIV GGNNCC 5 cut(s) 57, 277, 866, 900, 1110
NmeAIII GCCGAG 1 cut(s) 1087
NspI RCATGY 3 cut(s) 265, 499, 950
PaeI GCATGC 1 cut(s) 499
PctI GAATGC 1 cut(s) 660
PfeI GAWTC 3 cut(s) 183, 697, 1014
PflMI CCANNNNNTGG 1 cut(s) 272
PkrI GCNGC 1 cut(s) 384
PleI GAGTC 2 cut(s) 18, 512
PpsI GAGTC 2 cut(s) 18, 512
PpuMI RGGWCCY 1 cut(s) 898
Psp5II RGGWCCY 1 cut(s) 898
Psp6I CCWGG 2 cut(s) 58, 984
PspGI CCWGG 2 cut(s) 58, 984
PspN4I GGNNCC 5 cut(s) 57, 277, 866, 900, 1110
PspPI GGNCC 4 cut(s) 767, 864, 898, 1108
PspPPI RGGWCCY 1 cut(s) 898
PstNI CAGNNNCTG 1 cut(s) 886
PvuII CAGCTG 1 cut(s) 857
RsaI GTAC 4 cut(s) 315, 569, 818, 1082
RsaNI GTAC 4 cut(s) 314, 568, 817, 1081
RseI CAYNNNNRTG 1 cut(s) 608
SaqAI TTAA 2 cut(s) 210, 321
SatI GCNGC 1 cut(s) 383
Sau96I GGNCC 4 cut(s) 767, 864, 898, 1108
SchI GAGTC 2 cut(s) 18, 512
ScrFI CCNGG 4 cut(s) 60, 620, 666, 986
SfaNI GCATC 2 cut(s) 112, 873
SfcI CTRYAG 1 cut(s) 387
SinI GGWCC 3 cut(s) 864, 898, 1108
SmiMI CAYNNNNRTG 1 cut(s) 608
SmlI CTYRAG 3 cut(s) 209, 941, 954
SmoI CTYRAG 3 cut(s) 209, 941, 954
SphI GCATGC 1 cut(s) 499
SsiI CCGC 2 cut(s) 795, 1031
StyD4I CCNGG 4 cut(s) 58, 618, 664, 984
StyI CCWWGG 3 cut(s) 231, 280, 770
TaaI ACNGT 2 cut(s) 313, 642
TaqI TCGA 3 cut(s) 513, 597, 1119
TatI WGTACW 3 cut(s) 313, 567, 1080
TfiI GAWTC 3 cut(s) 183, 697, 1014
Tru1I TTAA 2 cut(s) 210, 321
Tru9I TTAA 2 cut(s) 210, 321
TscAI CASTG 5 cut(s) 57, 105, 316, 427, 610
TseI GCWGC 1 cut(s) 382
TspGWI ACGGA 1 cut(s) 491
TspRI CASTG 5 cut(s) 57, 105, 316, 427, 610
Van91I CCANNNNNTGG 1 cut(s) 272
Vha464I CTTAAG 1 cut(s) 209
VpaK11BI GGWCC 3 cut(s) 864, 898, 1108
XagI CCTNNNNNAGG 1 cut(s) 137
XapI RAATTY 3 cut(s) 359, 409, 462
XceI RCATGY 3 cut(s) 265, 499, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.