MD07G1036400.v1.1

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001519
Physical Location & Seq
Forward (+)
3010178 .. 3015568
5391 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1036400.v1.1.491

Sequence Viewer

Length: 1356 bp
ATGGCACTCATTTCACAGACAGCTTCACTATATTATGCTGCTAGGAAGACACAGATTTTGCATGTAGCAGGCAACTTAGACGGAGGCCAGAAACGATGGCGTAGCGTGATCGTTAATGCAAGGCCTCCCTCAGAAGCAGCTGCAGTTTCAACAGATCCGCTGACCAAGGAGGATCTCATAGGCTACTTTGCCTCTGGATGCAAGCCTAAGGAAAATTGGAGGATTGGGATTGAACATGAGAGGTTTGGGTTTGACCTAAAAACTTTACGTCCAATGAAGTATGAACAAATAGCTGAGTTGCTTAATGGGATTGCTGAGAGATTTGGTTGGGATAAAATTTTTGAAGGTTACAACGTTATTGCACTTAAACAGGGTAACCAAAGCATAGCGCTGGAGCCTGGAGGTCAGTTCGAGCATAGCGGTGCACCCCTTGAAACTCTGCATCAAATTCATGCTGAAGTTGATTCACACCTTTATCAGGTTCAATCGGTTCTAAAGGAAATAGGAATTGGATTACTTGATATTGGTTATCAGCCTAAATGGGAGATTAAAGACATACCTTTGATGCCCAAGGAAAGATATCAAGTTTTTCTAAATTACATGCCCAAAGTTGGCTCTTCGGAAACAGATATGATAAGGAAACTCCGTGCTAGTCTTGCTTTGCAGCCTATTGCAACAGCTCTATTTGCAAATTCACCTTTCACTGAAGGAAAGCCAAGTGGTTTTCTTTGCACGAGAAGTGAAATTTATACTGATGTGGACAAGGATCGTACAGGCATGCTTCCTTTTGTTTTTCATGACTCCTTTGGGTTTGAGCGCTATGTTGATTATGCTCTTGATATTCCCATGTATTTTGTCTACCGGAACAAGAACTATTTTGACTGTACTGACATGACCTTCCGGGAGTTTATGGCAGGAAAGCGTTGTTGCATTCCGGGTGAAGTTATTCCAACCATAAGTGATTGGGAGATGCATTTGGGAACTATATATCCTGAGGTTCATTTCATCATACGTGCGCTGAGGAAGTACTTGGAGATGAGGGGCGCTGGTTCAGGGCCTTGGAAGACATTTTGTGGTTTGCCAGCATTTTGGACTGGTTTATTGTATGATGAAGTTTCCCTACAAAATGTGTTGGACATGATAGCTGACTGGACCCCCGAAGAAAGACAGATGCTGAGAGATAAGGTCCCCAAAACTGGTCTAAAGACGCCATTTCGTGGAGAACTGCTGCAGCATGTTGCCGAAAATGTGGTGAAGTTTGCCAAGGATGGCCTGGAAAGAAGAGGCTTGAATGAATCGGGATTTTTGGATGGGGTGGTGGAGGTGGCGAGAACTGGTGTAGGTCTTCGCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

452

Amino Acids

51.17

Weight (kDa)

6.25

Isoelectric Point (pI)

38.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 74 - 434 5.5e-70 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1217
AccI GTMKAC 1 cut(s) 858
AciI CCGC 2 cut(s) 158, 420
AclI AACGTT 1 cut(s) 354
AclWI GGATC 3 cut(s) 149, 180, 774
AcsI RAATTY 4 cut(s) 336, 447, 691, 744
AcuI CTGAAG 2 cut(s) 477, 726
AcyI GRCGYC 1 cut(s) 1208
AfaI GTAC 3 cut(s) 772, 886, 1028
AfeI AGCGCT 2 cut(s) 390, 818
AfiI CCNNNNNNNGG 4 cut(s) 478, 611, 1196, 1217
AgsI TTSAA 6 cut(s) 150, 233, 344, 434, 485, 1291
AjnI CCWGG 2 cut(s) 397, 1272
AjuI GAANNNNNNNTTGG 2 cut(s) 492, 524
AloI GAACNNNNNNTCC 2 cut(s) 973, 1005
AluBI AGCT 5 cut(s) 23, 140, 293, 680, 1145
AluI AGCT 5 cut(s) 23, 140, 293, 680, 1145
Alw21I GWGCWC 1 cut(s) 427
Alw44I GTGCAC 1 cut(s) 423
AlwI GGATC 3 cut(s) 149, 180, 774
AlwNI CAGNNNCTG 1 cut(s) 1174
Aor51HI AGCGCT 2 cut(s) 390, 818
AoxI GGCC 4 cut(s) 85, 122, 1055, 1270
ApaLI GTGCAC 1 cut(s) 423
ApeKI GCWGC 6 cut(s) 38, 137, 140, 664, 1228, 1231
ApoI RAATTY 4 cut(s) 336, 447, 691, 744
ArsI GACNNNNNNTTYG 2 cut(s) 40, 72
Asp700I GAANNNNTTC 1 cut(s) 945
AspLEI GCGC 4 cut(s) 391, 819, 1018, 1046
AspS9I GGNCC 3 cut(s) 1055, 1152, 1186
AsuC2I CCSGG 2 cut(s) 902, 936
AsuHPI GGTGA 3 cut(s) 687, 950, 1264
AvaII GGWCC 2 cut(s) 1152, 1186
AxyI CCTNAGG 2 cut(s) 207, 993
BaeGI GKGCMC 1 cut(s) 427
BarI GAAGNNNNNNTAC 2 cut(s) 1104, 1136
BauI CACGAG 1 cut(s) 733
BbsI GAAGAC 3 cut(s) 53, 1070, 1337
Bbv12I GWGCWC 1 cut(s) 427
BbvCI CCTCAGC 1 cut(s) 1019
BbvI GCAGC 6 cut(s) 25, 127, 149, 676, 1215, 1243
BccI CCATC 3 cut(s) 90, 1262, 1304
BciT130I CCWGG 2 cut(s) 399, 1274
BcnI CCSGG 2 cut(s) 902, 936
BfaI CTAG 2 cut(s) 42, 651
BfmI CTRYAG 2 cut(s) 141, 1229
BfoI RGCGCY 3 cut(s) 392, 820, 1047
BisI GCNGC 6 cut(s) 39, 138, 141, 665, 1229, 1232
BlsI GCNGC 6 cut(s) 40, 139, 142, 666, 1230, 1233
BmcAI AGTACT 1 cut(s) 1028
Bme1390I CCNGG 4 cut(s) 399, 902, 936, 1274
Bme18I GGWCC 2 cut(s) 1152, 1186
BmgT120I GGNCC 3 cut(s) 1055, 1152, 1186
BmiI GGNNCC 3 cut(s) 396, 1154, 1188
BmrFI CCNGG 4 cut(s) 399, 902, 936, 1274
BmsI GCATC 5 cut(s) 188, 451, 555, 960, 1161
BpiI GAAGAC 3 cut(s) 53, 1070, 1337
BpmI CTGGAG 2 cut(s) 413, 420
Bpu10I CCTNAGC 1 cut(s) 1019
BpuMI CCSGG 2 cut(s) 902, 936
BsaAI YACGTR 1 cut(s) 1013
BsaHI GRCGYC 1 cut(s) 1208
BsaJI CCNNGG 4 cut(s) 165, 570, 1058, 1263
BsaWI WCCGGW 1 cut(s) 861
Bsc4I CCNNNNNNNGG 4 cut(s) 478, 611, 1196, 1217
Bse1I ACTGG 4 cut(s) 1099, 1154, 1201, 1339
Bse21I CCTNAGG 2 cut(s) 207, 993
BseBI CCWGG 2 cut(s) 399, 1274
BseDI CCNNGG 4 cut(s) 165, 570, 1058, 1263
BseGI GGATG 3 cut(s) 203, 1273, 1315
BseLI CCNNNNNNNGG 4 cut(s) 478, 611, 1196, 1217
BseMII CTCAG 6 cut(s) 144, 285, 306, 984, 1010, 1166
BseNI ACTGG 4 cut(s) 1099, 1154, 1201, 1339
BseSI GKGCMC 1 cut(s) 427
BseXI GCAGC 6 cut(s) 25, 127, 149, 676, 1215, 1243
BshFI GGCC 4 cut(s) 87, 124, 1057, 1272
BsiHKAI GWGCWC 1 cut(s) 427
BsiSI CCGG 3 cut(s) 862, 901, 935
BslFI GGGAC 1 cut(s) 1172
BslI CCNNNNNNNGG 4 cut(s) 478, 611, 1196, 1217
BsmFI GGGAC 1 cut(s) 1172
BsmI GAATGC 1 cut(s) 930
BsnI GGCC 4 cut(s) 87, 124, 1057, 1272
Bsp1286I GDGCHC 1 cut(s) 427
Bsp143I GATC 4 cut(s) 108, 154, 172, 766
BspACI CCGC 2 cut(s) 158, 420
BspANI GGCC 4 cut(s) 87, 124, 1057, 1272
BspCNI CTCAG 6 cut(s) 143, 286, 307, 985, 1011, 1167
BspHI TCATGA 1 cut(s) 796
BspLI GGNNCC 3 cut(s) 396, 1154, 1188
BspMAI CTGCAG 2 cut(s) 145, 1233
BspPI GGATC 3 cut(s) 149, 180, 774
BspQI GCTCTTC 1 cut(s) 622
BsrI ACTGG 4 cut(s) 1099, 1154, 1201, 1339
BssECI CCNNGG 4 cut(s) 165, 570, 1058, 1263
BssMI GATC 4 cut(s) 108, 154, 172, 766
BssNI GRCGYC 1 cut(s) 1208
BssSI CACGAG 1 cut(s) 733
BssT1I CCWWGG 4 cut(s) 165, 570, 1058, 1263
Bst2BI CACGAG 1 cut(s) 733
Bst2UI CCWGG 2 cut(s) 399, 1274
Bst4CI ACNGT 1 cut(s) 884
Bst6I CTCTTC 2 cut(s) 622, 1276
BstACI GRCGYC 1 cut(s) 1208
BstBAI YACGTR 1 cut(s) 1013
BstC8I GCNNGC 4 cut(s) 70, 203, 779, 1083
BstDEI CTNAG 8 cut(s) 76, 130, 207, 294, 315, 993, 1019, 1175
BstEII GGTNACC 1 cut(s) 374
BstENI CCTNNNNNAGG 1 cut(s) 476
BstF5I GGATG 3 cut(s) 203, 1273, 1315
BstH2I RGCGCY 3 cut(s) 392, 820, 1047
BstHHI GCGC 4 cut(s) 391, 819, 1018, 1046
BstKTI GATC 4 cut(s) 111, 157, 175, 769
BstMBI GATC 4 cut(s) 108, 154, 172, 766
BstMWI GCNNNNNNNGC 2 cut(s) 656, 686
BstNI CCWGG 2 cut(s) 399, 1274
BstNSI RCATGY 4 cut(s) 65, 604, 781, 1238
BstPI GGTNACC 1 cut(s) 374
BstSCI CCNGG 4 cut(s) 397, 900, 934, 1272
BstSFI CTRYAG 2 cut(s) 141, 1229
BstSLI GKGCMC 1 cut(s) 427
BstV1I GCAGC 6 cut(s) 25, 127, 149, 676, 1215, 1243
BstV2I GAAGAC 3 cut(s) 53, 1070, 1337
BstX2I RGATCY 2 cut(s) 154, 172
BstXI CCANNNNNNTGG 1 cut(s) 1089
BstYI RGATCY 2 cut(s) 154, 172
Bsu36I CCTNAGG 2 cut(s) 207, 993
BsuRI GGCC 4 cut(s) 87, 124, 1057, 1272
BtsCI GGATG 3 cut(s) 203, 1273, 1315
BtsIMutI CAGTG 1 cut(s) 702
Cac8I GCNNGC 4 cut(s) 70, 203, 779, 1083
CaiI CAGNNNCTG 1 cut(s) 1174
CciI TCATGA 1 cut(s) 796
CfoI GCGC 4 cut(s) 391, 819, 1018, 1046
Cfr13I GGNCC 3 cut(s) 1055, 1152, 1186
CseI GACGC 1 cut(s) 1216
Csp6I GTAC 3 cut(s) 771, 885, 1027
CviQI GTAC 3 cut(s) 771, 885, 1027
DdeI CTNAG 8 cut(s) 76, 130, 207, 294, 315, 993, 1019, 1175
DpnI GATC 4 cut(s) 110, 156, 174, 768
DpnII GATC 4 cut(s) 108, 154, 172, 766
Eam1104I CTCTTC 2 cut(s) 622, 1276
EarI CTCTTC 2 cut(s) 622, 1276
Eco130I CCWWGG 4 cut(s) 165, 570, 1058, 1263
Eco147I AGGCCT 1 cut(s) 124
Eco32I GATATC 1 cut(s) 581
Eco47I GGWCC 2 cut(s) 1152, 1186
Eco47III AGCGCT 2 cut(s) 390, 818
Eco57I CTGAAG 2 cut(s) 477, 726
Eco81I CCTNAGG 2 cut(s) 207, 993
Eco91I GGTNACC 1 cut(s) 374
EcoNI CCTNNNNNAGG 1 cut(s) 476
EcoO109I RGGNCCY 2 cut(s) 1055, 1186
EcoO65I GGTNACC 1 cut(s) 374
EcoRII CCWGG 2 cut(s) 397, 1272
EcoRV GATATC 1 cut(s) 581
EcoT14I CCWWGG 4 cut(s) 165, 570, 1058, 1263
EcoT22I ATGCAT 1 cut(s) 975
ErhI CCWWGG 4 cut(s) 165, 570, 1058, 1263
FaqI GGGAC 1 cut(s) 1172
FblI GTMKAC 1 cut(s) 858
Fnu4HI GCNGC 6 cut(s) 39, 138, 141, 665, 1229, 1232
FokI GGATG 3 cut(s) 210, 1280, 1322
Fsp4HI GCNGC 6 cut(s) 39, 138, 141, 665, 1229, 1232
FspBI CTAG 2 cut(s) 42, 651
GlaI GCGC 4 cut(s) 390, 818, 1017, 1045
GluI GCNGC 6 cut(s) 39, 138, 141, 665, 1229, 1232
GsuI CTGGAG 2 cut(s) 413, 420
HaeII RGCGCY 3 cut(s) 392, 820, 1047
HaeIII GGCC 4 cut(s) 87, 124, 1057, 1272
HapII CCGG 3 cut(s) 862, 901, 935
HgaI GACGC 1 cut(s) 1216
HhaI GCGC 4 cut(s) 391, 819, 1018, 1046
Hin1I GRCGYC 1 cut(s) 1208
Hin6I GCGC 4 cut(s) 389, 817, 1016, 1044
HinP1I GCGC 4 cut(s) 389, 817, 1016, 1044
HinfI GANTC 3 cut(s) 464, 800, 1295
HpaII CCGG 3 cut(s) 862, 901, 935
HphI GGTGA 3 cut(s) 687, 950, 1264
Hpy166II GTNNAC 3 cut(s) 425, 760, 859
Hpy188I TCNGA 2 cut(s) 133, 622
Hpy188III TCNNGA 5 cut(s) 195, 797, 836, 992, 1299
Hpy8I GTNNAC 3 cut(s) 425, 760, 859
HpyAV CCTTC 3 cut(s) 338, 701, 907
HpyCH4III ACNGT 1 cut(s) 884
HpyCH4IV ACGT 3 cut(s) 268, 354, 1012
HpyF10VI GCNNNNNNNGC 2 cut(s) 656, 686
HpyF3I CTNAG 8 cut(s) 76, 130, 207, 294, 315, 993, 1019, 1175
HpySE526I ACGT 3 cut(s) 268, 354, 1012
Hsp92I GRCGYC 1 cut(s) 1208
HspAI GCGC 4 cut(s) 389, 817, 1016, 1044
Kzo9I GATC 4 cut(s) 108, 154, 172, 766
LguI GCTCTTC 1 cut(s) 622
LmnI GCTCC 1 cut(s) 394
Lsp1109I GCAGC 6 cut(s) 25, 127, 149, 676, 1215, 1243
LweI GCATC 5 cut(s) 188, 451, 555, 960, 1161
MaeI CTAG 2 cut(s) 42, 651
MaeII ACGT 3 cut(s) 268, 354, 1012
MaeIII GTNAC 2 cut(s) 347, 374
MalI GATC 4 cut(s) 110, 156, 174, 768
MboI GATC 4 cut(s) 108, 154, 172, 766
MboII GAAGA 6 cut(s) 58, 609, 1075, 1172, 1293, 1337
MflI RGATCY 2 cut(s) 154, 172
MhlI GDGCHC 1 cut(s) 427
MluCI AATT 7 cut(s) 214, 336, 447, 507, 595, 691, 744
MlyI GAGTC 1 cut(s) 794
MmeI TCCRAC 2 cut(s) 974, 1113
Mph1103I ATGCAT 1 cut(s) 975
MroXI GAANNNNTTC 1 cut(s) 945
MseI TTAA 4 cut(s) 114, 303, 366, 549
MslI CAYNNNNRTG 1 cut(s) 420
MspA1I CMGCKG 2 cut(s) 140, 160
MspI CCGG 3 cut(s) 862, 901, 935
MspR9I CCNGG 4 cut(s) 399, 902, 936, 1274
Mva1269I GAATGC 1 cut(s) 930
MvaI CCWGG 2 cut(s) 399, 1274
MwoI GCNNNNNNNGC 2 cut(s) 656, 686
NciI CCSGG 2 cut(s) 902, 936
NdeII GATC 4 cut(s) 108, 154, 172, 766
NlaIV GGNNCC 3 cut(s) 396, 1154, 1188
NsiI ATGCAT 1 cut(s) 975
NspI RCATGY 4 cut(s) 65, 604, 781, 1238
PaeI GCATGC 1 cut(s) 781
PagI TCATGA 1 cut(s) 796
PceI AGGCCT 1 cut(s) 124
PciSI GCTCTTC 1 cut(s) 622
PctI GAATGC 1 cut(s) 930
PdmI GAANNNNTTC 1 cut(s) 945
PfeI GAWTC 2 cut(s) 464, 1295
PflMI CCANNNNNTGG 1 cut(s) 1217
PfoI TCCNGGA 1 cut(s) 900
PkrI GCNGC 6 cut(s) 40, 139, 142, 666, 1230, 1233
PleI GAGTC 1 cut(s) 794
PpsI GAGTC 1 cut(s) 794
Ppu21I YACGTR 1 cut(s) 1013
PpuMI RGGWCCY 1 cut(s) 1186
Psp1406I AACGTT 1 cut(s) 354
Psp5II RGGWCCY 1 cut(s) 1186
Psp6I CCWGG 2 cut(s) 397, 1272
PspEI GGTNACC 1 cut(s) 374
PspGI CCWGG 2 cut(s) 397, 1272
PspN4I GGNNCC 3 cut(s) 396, 1154, 1188
PspPI GGNCC 3 cut(s) 1055, 1152, 1186
PspPPI RGGWCCY 1 cut(s) 1186
PstI CTGCAG 2 cut(s) 145, 1233
PstNI CAGNNNCTG 1 cut(s) 1174
PsuI RGATCY 2 cut(s) 154, 172
PvuII CAGCTG 1 cut(s) 140
RsaI GTAC 3 cut(s) 772, 886, 1028
RsaNI GTAC 3 cut(s) 771, 885, 1027
RseI CAYNNNNRTG 1 cut(s) 420
SapI GCTCTTC 1 cut(s) 622
SaqAI TTAA 4 cut(s) 114, 303, 366, 549
SatI GCNGC 6 cut(s) 39, 138, 141, 665, 1229, 1232
Sau3AI GATC 4 cut(s) 108, 154, 172, 766
Sau96I GGNCC 3 cut(s) 1055, 1152, 1186
ScaI AGTACT 1 cut(s) 1028
SchI GAGTC 1 cut(s) 794
ScrFI CCNGG 4 cut(s) 399, 902, 936, 1274
SduI GDGCHC 1 cut(s) 427
SfaNI GCATC 5 cut(s) 188, 451, 555, 960, 1161
SfcI CTRYAG 2 cut(s) 141, 1229
SinI GGWCC 2 cut(s) 1152, 1186
SmiMI CAYNNNNRTG 1 cut(s) 420
SphI GCATGC 1 cut(s) 781
Sse9I AATT 7 cut(s) 214, 336, 447, 507, 595, 691, 744
SseBI AGGCCT 1 cut(s) 124
SsiI CCGC 2 cut(s) 158, 420
SspMI CTAG 2 cut(s) 42, 651
StuI AGGCCT 1 cut(s) 124
StyD4I CCNGG 4 cut(s) 397, 900, 934, 1272
StyI CCWWGG 4 cut(s) 165, 570, 1058, 1263
TaaI ACNGT 1 cut(s) 884
TaiI ACGT 3 cut(s) 271, 357, 1015
TaqI TCGA 1 cut(s) 411
TasI AATT 7 cut(s) 214, 336, 447, 507, 595, 691, 744
TatI WGTACW 2 cut(s) 884, 1026
TfiI GAWTC 2 cut(s) 464, 1295
Tru1I TTAA 4 cut(s) 114, 303, 366, 549
Tru9I TTAA 4 cut(s) 114, 303, 366, 549
TscAI CASTG 1 cut(s) 709
TseI GCWGC 6 cut(s) 38, 137, 140, 664, 1228, 1231
TspDTI ATGAA 8 cut(s) 290, 297, 440, 785, 989, 994, 1125, 1308
TspGWI ACGGA 2 cut(s) 96, 635
TspRI CASTG 1 cut(s) 709
Van91I CCANNNNNTGG 1 cut(s) 1217
VneI GTGCAC 1 cut(s) 423
VpaK11BI GGWCC 2 cut(s) 1152, 1186
XagI CCTNNNNNAGG 1 cut(s) 476
XapI RAATTY 4 cut(s) 336, 447, 691, 744
XceI RCATGY 4 cut(s) 65, 604, 781, 1238
XcmI CCANNNNNNNNNTGG 1 cut(s) 1270
XmiI GTMKAC 1 cut(s) 858
XmnI GAANNNNTTC 1 cut(s) 945
XspI CTAG 2 cut(s) 42, 651
ZrmI AGTACT 1 cut(s) 1028
Zsp2I ATGCAT 1 cut(s) 975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.