Rroxscaffold_3G00258220

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001519
Physical Location & Seq
Reverse (-)
52524901 .. 52537900
13000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00258220.1

Sequence Viewer

Length: 504 bp
ATGCTTATTCGTGCTCAGGGAAGATATGAGATTATTAGAAATTACATGCCAAAAGTTGGCTCGCTTGGACATGATTTGATGTTCAGAACTTGCACTCTACAGGTCAGGCTGAAGAGATTCTTGGAGATGAGGGGGAGGACCTTGGAGAAGCTTATGCTCTTGGCCTGCATTTTGGTGGGTCTACTATATGATGAAGTTTCCCTGCAGAATGCGTTAGACATGATTGCTGATTGGAATCCTGAAGAAAGACAGATGCTGAGAGATAAGATTCCAAAAACGGGTCTAAAGACACCATTTCGTGAAGGAATACTCAAGCATGTTGCCGAAGATGTAGTAAAGTTTGCAAAGTACCCAGGTTATGTAAGGAAAGATTTGAAGGACATGCTGCAAATATTTCCAGCAAGTGCATGCGGTGAAACTATGGAACAAATGAGACGTTCGAGTATTGATTCTGACCAGACTAAGCTTACAAATTTTGCTAATTGTCTTGGGAAATCGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

167

Amino Acids

19.28

Weight (kDa)

9.34

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 56
AccI GTMKAC 1 cut(s) 181
AciI CCGC 1 cut(s) 411
AcsI RAATTY 1 cut(s) 472
AcuI CTGAAG 2 cut(s) 131, 261
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 2 cut(s) 56, 278
AgsI TTSAA 1 cut(s) 376
AjnI CCWGG 1 cut(s) 352
AjuI GAANNNNNNNTTGG 2 cut(s) 104, 136
AluBI AGCT 2 cut(s) 151, 466
AluI AGCT 2 cut(s) 151, 466
Alw21I GWGCWC 1 cut(s) 16
Alw26I GTCTC 1 cut(s) 427
AlwNI CAGNNNCTG 1 cut(s) 256
AoxI GGCC 1 cut(s) 162
ApeKI GCWGC 1 cut(s) 385
ApoI RAATTY 1 cut(s) 472
Asp700I GAANNNNTTC 1 cut(s) 116
AspS9I GGNCC 1 cut(s) 138
AsuHPI GGTGA 1 cut(s) 425
AvaII GGWCC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 16
BbvI GCAGC 1 cut(s) 372
BciT130I CCWGG 1 cut(s) 354
BcoDI GTCTC 1 cut(s) 427
BfmI CTRYAG 2 cut(s) 98, 203
BisI GCNGC 1 cut(s) 386
BlsI GCNGC 1 cut(s) 387
Bme1390I CCNGG 1 cut(s) 354
Bme18I GGWCC 1 cut(s) 138
BmgT120I GGNCC 1 cut(s) 138
BmrFI CCNGG 1 cut(s) 354
BmsI GCATC 1 cut(s) 243
Bpu10I CCTNAGC 1 cut(s) 15
BpuEI CTTGAG 1 cut(s) 296
BsaBI GATNNNNATC 1 cut(s) 234
BsaJI CCNNGG 2 cut(s) 141, 352
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 278
Bse8I GATNNNNATC 1 cut(s) 234
BseBI CCWGG 1 cut(s) 354
BseDI CCNNGG 2 cut(s) 141, 352
BseJI GATNNNNATC 1 cut(s) 234
BseLI CCNNNNNNNGG 2 cut(s) 56, 278
BseMII CTCAG 2 cut(s) 29, 248
BseXI GCAGC 1 cut(s) 372
BshFI GGCC 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 16
BslI CCNNNNNNNGG 2 cut(s) 56, 278
BsmAI GTCTC 1 cut(s) 427
BsmBI CGTCTC 1 cut(s) 427
BsmI GAATGC 1 cut(s) 214
BsnI GGCC 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 16
BspACI CCGC 1 cut(s) 411
BspANI GGCC 1 cut(s) 164
BspCNI CTCAG 2 cut(s) 28, 249
BspMAI CTGCAG 1 cut(s) 207
BssECI CCNNGG 2 cut(s) 141, 352
BssT1I CCWWGG 1 cut(s) 141
Bst2UI CCWGG 1 cut(s) 354
Bst6I CTCTTC 1 cut(s) 107
BstC8I GCNNGC 3 cut(s) 62, 166, 409
BstDEI CTNAG 3 cut(s) 15, 257, 462
BstMAI GTCTC 1 cut(s) 427
BstNI CCWGG 1 cut(s) 354
BstNSI RCATGY 4 cut(s) 49, 320, 385, 411
BstSCI CCNGG 1 cut(s) 352
BstSFI CTRYAG 2 cut(s) 98, 203
BstV1I GCAGC 1 cut(s) 372
BsuRI GGCC 1 cut(s) 164
Cac8I GCNNGC 3 cut(s) 62, 166, 409
CaiI CAGNNNCTG 1 cut(s) 256
Cfr13I GGNCC 1 cut(s) 138
Csp6I GTAC 1 cut(s) 349
CviAII CATG 6 cut(s) 46, 71, 220, 317, 382, 408
CviJI RGCY 5 cut(s) 60, 109, 151, 164, 466
CviKI_1 RGCY 5 cut(s) 60, 109, 151, 164, 466
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 3 cut(s) 15, 257, 462
Eam1104I CTCTTC 1 cut(s) 107
EarI CTCTTC 1 cut(s) 107
Eco130I CCWWGG 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 138
Eco57I CTGAAG 2 cut(s) 131, 261
EcoO109I RGGNCCY 1 cut(s) 138
EcoRII CCWGG 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
Esp3I CGTCTC 1 cut(s) 427
FaeI CATG 6 cut(s) 49, 74, 223, 320, 385, 411
FalI AAGNNNNNCTT 2 cut(s) 104, 136
FatI CATG 6 cut(s) 45, 70, 219, 316, 381, 407
FblI GTMKAC 1 cut(s) 181
Fnu4HI GCNGC 1 cut(s) 386
Fsp4HI GCNGC 1 cut(s) 386
GluI GCNGC 1 cut(s) 386
HaeIII GGCC 1 cut(s) 164
Hin1II CATG 6 cut(s) 49, 74, 223, 320, 385, 411
HindIII AAGCTT 2 cut(s) 149, 464
HinfI GANTC 4 cut(s) 117, 235, 268, 449
HphI GGTGA 1 cut(s) 425
Hpy166II GTNNAC 1 cut(s) 182
Hpy188I TCNGA 2 cut(s) 86, 454
Hpy188III TCNNGA 2 cut(s) 239, 299
Hpy8I GTNNAC 1 cut(s) 182
HpyAV CCTTC 2 cut(s) 296, 370
HpyCH4IV ACGT 1 cut(s) 436
HpyCH4V TGCA 6 cut(s) 93, 168, 205, 344, 388, 407
HpyF3I CTNAG 3 cut(s) 15, 257, 462
HpySE526I ACGT 1 cut(s) 436
Hsp92II CATG 6 cut(s) 49, 74, 223, 320, 385, 411
Lsp1109I GCAGC 1 cut(s) 372
LweI GCATC 1 cut(s) 243
MaeII ACGT 1 cut(s) 436
MboII GAAGA 4 cut(s) 33, 124, 254, 338
MhlI GDGCHC 1 cut(s) 16
MluCI AATT 3 cut(s) 40, 472, 481
MnlI CCTC 2 cut(s) 123, 129
MroXI GAANNNNTTC 1 cut(s) 116
MslI CAYNNNNRTG 1 cut(s) 173
MspR9I CCNGG 1 cut(s) 354
Mva1269I GAATGC 1 cut(s) 214
MvaI CCWGG 1 cut(s) 354
NlaIII CATG 6 cut(s) 49, 74, 223, 320, 385, 411
NspI RCATGY 4 cut(s) 49, 320, 385, 411
PaeI GCATGC 1 cut(s) 411
PctI GAATGC 1 cut(s) 214
PdmI GAANNNNTTC 1 cut(s) 116
PfeI GAWTC 4 cut(s) 117, 235, 268, 449
PflMI CCANNNNNTGG 1 cut(s) 56
PkrI GCNGC 1 cut(s) 387
PpuMI RGGWCCY 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 352
PspGI CCWGG 1 cut(s) 352
PspPI GGNCC 1 cut(s) 138
PspPPI RGGWCCY 1 cut(s) 138
PstI CTGCAG 1 cut(s) 207
PstNI CAGNNNCTG 1 cut(s) 256
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
RseI CAYNNNNRTG 1 cut(s) 173
SatI GCNGC 1 cut(s) 386
Sau96I GGNCC 1 cut(s) 138
ScrFI CCNGG 1 cut(s) 354
SduI GDGCHC 1 cut(s) 16
SetI ASST 6 cut(s) 105, 143, 153, 358, 439, 468
SfaNI GCATC 1 cut(s) 243
SfcI CTRYAG 2 cut(s) 98, 203
SinI GGWCC 1 cut(s) 138
SmiMI CAYNNNNRTG 1 cut(s) 173
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
SphI GCATGC 1 cut(s) 411
Sse9I AATT 3 cut(s) 40, 472, 481
SsiI CCGC 1 cut(s) 411
SspI AATATT 1 cut(s) 393
StyD4I CCNGG 1 cut(s) 352
StyI CCWWGG 1 cut(s) 141
TaiI ACGT 1 cut(s) 439
TaqI TCGA 2 cut(s) 440, 497
TasI AATT 3 cut(s) 40, 472, 481
TfiI GAWTC 4 cut(s) 117, 235, 268, 449
TseI GCWGC 1 cut(s) 385
TspDTI ATGAA 1 cut(s) 207
Van91I CCANNNNNTGG 1 cut(s) 56
VpaK11BI GGWCC 1 cut(s) 138
XapI RAATTY 1 cut(s) 472
XceI RCATGY 4 cut(s) 49, 320, 385, 411
XmiI GTMKAC 1 cut(s) 181
XmnI GAANNNNTTC 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.