Rw7G014560

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001519
Physical Location & Seq
Forward (+)
14251075 .. 14257260
6186 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G014560.1

Sequence Viewer

Length: 1581 bp
ATGGCACTCGTTACACAGACAGGCTCGTCATACTGCATGCGTAACGAGATGTTTCAGAATAAAACTGGACAAAATGTGGCGTTTAGTAGGGCAAGCAATATAGAGGCGTCCAAATTGAAGGACAACTGCATTGGCTTCTCTTCGTTATTGTGTAATTCTGCTAGGAAGACACAGATTTTGCATACGGACAATGCTGGAGTAGGACAGAAACGACGCCATAGTGTGGTAGTTGCAGCCAGTCCTCCCACAGAAGATGCTGTAATTGCTACGGAACCGCTGACGAAAGAGGATCTTGTAGTGTATCTTGCCTCTGGATGCAAGCCTAAGGAAGCATGGAGAATTGGCACTGAACACGAGAAGTTTGGTTTTGAGCTCAAATCTTTACGTCCAGTGAAATATGAACAAATATCTGAGTTGCTTAATGGGATTGCCGAGAGATTTGATTGGGAAAAAGTTATGGAAGGTGACTACATTATTGGACTCAAACAGGGTAAGCAAAGTATATCACTTGAGCCTGGTGGCCAATTTGAGCTTAGTGGCGCACCTCTTGAAACTCTGCATCAAACTTGTGCTGAAGTCAATTCACACCTCTATCAGGTCAAAGCAGTTACAGAGGAAATGGGAATTGGATTTCTTGGCATTGGTTTTCAGCCTAAATGGGGACTGAAGGACATACCTATAATGCCCAAGGGAAGGTATGAGATTATGAAAAATTACATGCCCAAAGTTGGCACGCTTGGACTTGATATGATGTTCAGGACATGCACTGTACAGGTGAATCTGGACTTCAGCTCTGAAGCAGACATGATAAGGAAATTTCGTGCTGGTCTTGCTTTGCAGCCTATTGCAACAGCTCTATTTGCAAATTCACCTTTTACCGAAGGAAAGCCAAATGGTTTTCTAAGCATGAGAAGTCAAATTTGGACCGATACTGACAACAATCGCACTGGCATGCTTCCTTTTGTTTTTGATGACTCCTTTGGATTTGAGCAGTATGTTGATTATGCTCTTGATGTTCCCATGTACTTTGTCTATCGGAATAAAAAGTATATTGACTGTACTGGCATGACCTTCCGGGACTTTTTGGCTGGGAAGCTTTGTTGCATTCCTGGTGAATTGCCAACCCTGAACGATTGGGAGAATCATTTGACAACTATATTTCCTGAGGTCAGGCTGAAGAGATACTTGGAGATGAGGGGAGCTGATGGAGGGCCTTGGAGGAGATTATGTGCTTTGCCAGCATTTTGGGTAGGTATACTGTACGATGAGGTTTCCCTTCAAAATGTGTTGGACATTACTGCTGATTGGACCCCTGAAGAAAGACAGATGCTAAGAAATAAGGTTCCAATAACTGGTCTAAAGACACCATTTAGGGATGGATTGCTCAAGCATGTTGCTCAAGATGTTGTAAAGTTGGCAAAGGATGGACTTGAAAGAAGAGGCTTTAAGGAATCTGGCTTCTTGAATGAGGTGGCAGAGGTGGTTAGAACTGGTGTAACACCAGCTGAGAGATTATTGGAGTTGTACAATGGGAAGTGGGAACAACAGATAGACCCTGTTTTTGAAGAGCTACTATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

526

Amino Acids

59.43

Weight (kDa)

5.84

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 113 - 523 1.2e-106 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 25
AccB7I CCANNNNNTGG 2 cut(s) 223, 1352
AccI GTMKAC 1 cut(s) 1255
AciI CCGC 1 cut(s) 275
AclWI GGATC 1 cut(s) 297
AcoI YGGCCR 1 cut(s) 520
AcsI RAATTY 3 cut(s) 815, 865, 918
AcuI CTGAAG 6 cut(s) 594, 686, 772, 816, 1196, 1335
AcyI GRCGYC 2 cut(s) 107, 214
AfaI GTAC 5 cut(s) 771, 1025, 1060, 1262, 1526
AfiI CCNNNNNNNGG 6 cut(s) 223, 595, 659, 693, 728, 1352
AgsI TTSAA 6 cut(s) 118, 551, 1280, 1433, 1465, 1565
AjnI CCWGG 2 cut(s) 514, 1108
AjuI GAANNNNNNNTTGG 6 cut(s) 609, 641, 904, 936, 1169, 1201
AluBI AGCT 8 cut(s) 373, 532, 792, 854, 1096, 1202, 1505, 1570
AluI AGCT 8 cut(s) 373, 532, 792, 854, 1096, 1202, 1505, 1570
Alw21I GWGCWC 1 cut(s) 375
AlwI GGATC 1 cut(s) 297
AoxI GGCC 2 cut(s) 520, 1211
ApeKI GCWGC 2 cut(s) 233, 838
ApoI RAATTY 3 cut(s) 815, 865, 918
ArsI GACNNNNNNTTYG 4 cut(s) 160, 192, 1544, 1576
AspLEI GCGC 1 cut(s) 542
AspS9I GGNCC 3 cut(s) 924, 1211, 1308
AsuC2I CCSGG 1 cut(s) 1076
AsuHPI GGTGA 4 cut(s) 476, 787, 861, 1124
AvaII GGWCC 2 cut(s) 924, 1308
AxyI CCTNAGG 2 cut(s) 324, 1164
BalI TGGCCA 1 cut(s) 522
BanII GRGCYC 1 cut(s) 375
BauI CACGAG 1 cut(s) 353
BbsI GAAGAC 1 cut(s) 173
Bbv12I GWGCWC 1 cut(s) 375
BbvI GCAGC 2 cut(s) 245, 850
BccI CCATC 3 cut(s) 1199, 1370, 1418
BciT130I CCWGG 2 cut(s) 516, 1110
BcnI CCSGG 1 cut(s) 1076
BfaI CTAG 1 cut(s) 162
BisI GCNGC 2 cut(s) 234, 839
BlsI GCNGC 2 cut(s) 235, 840
Bme1390I CCNGG 3 cut(s) 516, 1076, 1110
Bme18I GGWCC 2 cut(s) 924, 1308
BmgT120I GGNCC 3 cut(s) 924, 1211, 1308
BmiI GGNNCC 3 cut(s) 273, 1310, 1344
BmrFI CCNGG 3 cut(s) 516, 1076, 1110
BmsI GCATC 4 cut(s) 244, 305, 568, 1317
BpiI GAAGAC 1 cut(s) 173
BpmI CTGGAG 1 cut(s) 216
BpuEI CTTGAG 3 cut(s) 530, 1370, 1383
BpuMI CCSGG 1 cut(s) 1076
BsaHI GRCGYC 2 cut(s) 107, 214
BsaJI CCNNGG 2 cut(s) 687, 1214
BsaXI ACNNNNNCTCC 2 cut(s) 1213, 1243
Bsc4I CCNNNNNNNGG 6 cut(s) 223, 595, 659, 693, 728, 1352
Bse1I ACTGG 7 cut(s) 70, 237, 389, 952, 1066, 1357, 1495
Bse21I CCTNAGG 2 cut(s) 324, 1164
BseBI CCWGG 2 cut(s) 516, 1110
BseDI CCNNGG 2 cut(s) 687, 1214
BseGI GGATG 3 cut(s) 320, 1381, 1429
BseLI CCNNNNNNNGG 6 cut(s) 223, 595, 659, 693, 728, 1352
BseMII CTCAG 3 cut(s) 402, 1155, 1497
BseNI ACTGG 7 cut(s) 70, 237, 389, 952, 1066, 1357, 1495
BseRI GAGGAG 1 cut(s) 1234
BseXI GCAGC 2 cut(s) 245, 850
BseYI CCCAGC 1 cut(s) 1088
BshFI GGCC 2 cut(s) 522, 1213
BsiHKAI GWGCWC 1 cut(s) 375
BsiSI CCGG 1 cut(s) 1075
BslFI GGGAC 2 cut(s) 675, 1091
BslI CCNNNNNNNGG 6 cut(s) 223, 595, 659, 693, 728, 1352
BsmFI GGGAC 2 cut(s) 675, 1091
BsmI GAATGC 1 cut(s) 1104
BsnI GGCC 2 cut(s) 522, 1213
Bsp1286I GDGCHC 1 cut(s) 375
Bsp1407I TGTACA 2 cut(s) 769, 1524
Bsp143I GATC 1 cut(s) 289
BspACI CCGC 1 cut(s) 275
BspANI GGCC 2 cut(s) 522, 1213
BspCNI CTCAG 3 cut(s) 403, 1156, 1498
BspLI GGNNCC 3 cut(s) 273, 1310, 1344
BspPI GGATC 1 cut(s) 297
BspQI GCTCTTC 1 cut(s) 1560
BsrGI TGTACA 2 cut(s) 769, 1524
BsrI ACTGG 7 cut(s) 70, 237, 389, 952, 1066, 1357, 1495
BssECI CCNNGG 2 cut(s) 687, 1214
BssMI GATC 1 cut(s) 289
BssNAI GTATAC 1 cut(s) 1256
BssNI GRCGYC 2 cut(s) 107, 214
BssSI CACGAG 1 cut(s) 353
BssT1I CCWWGG 2 cut(s) 687, 1214
Bst1107I GTATAC 1 cut(s) 1256
Bst2BI CACGAG 1 cut(s) 353
Bst2UI CCWGG 2 cut(s) 516, 1110
Bst4CI ACNGT 3 cut(s) 769, 1058, 1260
Bst6I CTCTTC 4 cut(s) 145, 1172, 1432, 1560
BstACI GRCGYC 2 cut(s) 107, 214
BstAUI TGTACA 2 cut(s) 769, 1524
BstC8I GCNNGC 6 cut(s) 38, 94, 320, 734, 953, 1239
BstDEI CTNAG 7 cut(s) 324, 411, 533, 902, 1164, 1331, 1506
BstENI CCTNNNNNAGG 1 cut(s) 593
BstF5I GGATG 3 cut(s) 320, 1381, 1429
BstHHI GCGC 1 cut(s) 542
BstKTI GATC 1 cut(s) 292
BstMBI GATC 1 cut(s) 289
BstMWI GCNNNNNNNGC 4 cut(s) 263, 830, 860, 1238
BstNI CCWGG 2 cut(s) 516, 1110
BstNSI RCATGY 5 cut(s) 40, 721, 765, 955, 1394
BstSCI CCNGG 3 cut(s) 514, 1074, 1108
BstV1I GCAGC 2 cut(s) 245, 850
BstV2I GAAGAC 1 cut(s) 173
BstX2I RGATCY 1 cut(s) 289
BstXI CCANNNNNNTGG 1 cut(s) 1245
BstYI RGATCY 1 cut(s) 289
BstZ17I GTATAC 1 cut(s) 1256
Bsu36I CCTNAGG 2 cut(s) 324, 1164
BsuRI GGCC 2 cut(s) 522, 1213
BtsCI GGATG 3 cut(s) 320, 1381, 1429
BtsIMutI CAGTG 4 cut(s) 345, 396, 765, 945
Cac8I GCNNGC 6 cut(s) 38, 94, 320, 734, 953, 1239
CfoI GCGC 1 cut(s) 542
Cfr13I GGNCC 3 cut(s) 924, 1211, 1308
CseI GACGC 2 cut(s) 96, 222
Csp6I GTAC 5 cut(s) 770, 1024, 1059, 1261, 1525
CviQI GTAC 5 cut(s) 770, 1024, 1059, 1261, 1525
DdeI CTNAG 7 cut(s) 324, 411, 533, 902, 1164, 1331, 1506
DpnI GATC 1 cut(s) 291
DpnII GATC 1 cut(s) 289
DrdI GACNNNNNNGTC 1 cut(s) 25
DseDI GACNNNNNNGTC 1 cut(s) 25
EaeI YGGCCR 1 cut(s) 520
Eam1104I CTCTTC 4 cut(s) 145, 1172, 1432, 1560
EarI CTCTTC 4 cut(s) 145, 1172, 1432, 1560
Ecl136II GAGCTC 1 cut(s) 373
Eco130I CCWWGG 2 cut(s) 687, 1214
Eco24I GRGCYC 1 cut(s) 375
Eco47I GGWCC 2 cut(s) 924, 1308
Eco53kI GAGCTC 1 cut(s) 373
Eco57I CTGAAG 6 cut(s) 594, 686, 772, 816, 1196, 1335
Eco81I CCTNAGG 2 cut(s) 324, 1164
EcoICRI GAGCTC 1 cut(s) 373
EcoNI CCTNNNNNAGG 1 cut(s) 593
EcoO109I RGGNCCY 1 cut(s) 1211
EcoRII CCWGG 2 cut(s) 514, 1108
EcoT14I CCWWGG 2 cut(s) 687, 1214
EcoT38I GRGCYC 1 cut(s) 375
ErhI CCWWGG 2 cut(s) 687, 1214
FalI AAGNNNNNCTT 4 cut(s) 276, 308, 1169, 1201
FaqI GGGAC 2 cut(s) 675, 1091
FblI GTMKAC 1 cut(s) 1255
Fnu4HI GCNGC 2 cut(s) 234, 839
FokI GGATG 3 cut(s) 327, 1388, 1436
FriOI GRGCYC 1 cut(s) 375
Fsp4HI GCNGC 2 cut(s) 234, 839
FspBI CTAG 1 cut(s) 162
GlaI GCGC 1 cut(s) 541
GluI GCNGC 2 cut(s) 234, 839
GsaI CCCAGC 1 cut(s) 1092
GsuI CTGGAG 1 cut(s) 216
HaeIII GGCC 2 cut(s) 522, 1213
HapII CCGG 1 cut(s) 1075
HgaI GACGC 2 cut(s) 96, 222
HhaI GCGC 1 cut(s) 542
Hin1I GRCGYC 2 cut(s) 107, 214
Hin6I GCGC 1 cut(s) 540
HinP1I GCGC 1 cut(s) 540
HindIII AAGCTT 1 cut(s) 1094
HinfI GANTC 5 cut(s) 480, 778, 974, 1141, 1451
HpaII CCGG 1 cut(s) 1075
HphI GGTGA 4 cut(s) 476, 787, 861, 1124
Hpy166II GTNNAC 1 cut(s) 1256
Hpy188I TCNGA 4 cut(s) 57, 412, 796, 1038
Hpy188III TCNNGA 8 cut(s) 312, 548, 757, 782, 1010, 1163, 1400, 1462
Hpy8I GTNNAC 1 cut(s) 1256
Hpy99I CGWCG 1 cut(s) 216
HpyAV CCTTC 7 cut(s) 112, 455, 661, 687, 875, 1081, 1286
HpyCH4III ACNGT 3 cut(s) 769, 1058, 1260
HpyCH4IV ACGT 1 cut(s) 385
HpyF10VI GCNNNNNNNGC 4 cut(s) 263, 830, 860, 1238
HpyF3I CTNAG 7 cut(s) 324, 411, 533, 902, 1164, 1331, 1506
HpySE526I ACGT 1 cut(s) 385
Hsp92I GRCGYC 2 cut(s) 107, 214
HspAI GCGC 1 cut(s) 540
Kzo9I GATC 1 cut(s) 289
LguI GCTCTTC 1 cut(s) 1560
LmnI GCTCC 1 cut(s) 1199
Lsp1109I GCAGC 2 cut(s) 245, 850
LweI GCATC 4 cut(s) 244, 305, 568, 1317
MaeI CTAG 1 cut(s) 162
MaeII ACGT 1 cut(s) 385
MaeIII GTNAC 5 cut(s) 10, 41, 464, 607, 1495
MalI GATC 1 cut(s) 291
MboI GATC 1 cut(s) 289
MboII GAAGA 7 cut(s) 132, 178, 263, 1189, 1328, 1449, 1577
MflI RGATCY 1 cut(s) 289
MhlI GDGCHC 1 cut(s) 375
MlsI TGGCCA 1 cut(s) 522
MluNI TGGCCA 1 cut(s) 522
MlyI GAGTC 2 cut(s) 474, 968
MmeI TCCRAC 1 cut(s) 1269
Mox20I TGGCCA 1 cut(s) 522
MscI TGGCCA 1 cut(s) 522
MseI TTAA 2 cut(s) 420, 1446
MslI CAYNNNNRTG 1 cut(s) 950
Msp20I TGGCCA 1 cut(s) 522
MspA1I CMGCKG 2 cut(s) 277, 1505
MspI CCGG 1 cut(s) 1075
MspR9I CCNGG 3 cut(s) 516, 1076, 1110
Mva1269I GAATGC 1 cut(s) 1104
MvaI CCWGG 2 cut(s) 516, 1110
MwoI GCNNNNNNNGC 4 cut(s) 263, 830, 860, 1238
NciI CCSGG 1 cut(s) 1076
NdeII GATC 1 cut(s) 289
NlaIV GGNNCC 3 cut(s) 273, 1310, 1344
NmeAIII GCCGAG 1 cut(s) 457
NmuCI GTSAC 1 cut(s) 464
NspI RCATGY 5 cut(s) 40, 721, 765, 955, 1394
PaeI GCATGC 2 cut(s) 40, 955
PciSI GCTCTTC 1 cut(s) 1560
PctI GAATGC 1 cut(s) 1104
PfeI GAWTC 3 cut(s) 778, 1141, 1451
PflMI CCANNNNNTGG 2 cut(s) 223, 1352
PfoI TCCNGGA 1 cut(s) 1074
PkrI GCNGC 2 cut(s) 235, 840
PleI GAGTC 2 cut(s) 474, 968
PpsI GAGTC 2 cut(s) 474, 968
Psp124BI GAGCTC 1 cut(s) 375
Psp6I CCWGG 2 cut(s) 514, 1108
PspFI CCCAGC 1 cut(s) 1088
PspGI CCWGG 2 cut(s) 514, 1108
PspN4I GGNNCC 3 cut(s) 273, 1310, 1344
PspPI GGNCC 3 cut(s) 924, 1211, 1308
PsuI RGATCY 1 cut(s) 289
PvuII CAGCTG 1 cut(s) 1505
RsaI GTAC 5 cut(s) 771, 1025, 1060, 1262, 1526
RsaNI GTAC 5 cut(s) 770, 1024, 1059, 1261, 1525
RseI CAYNNNNRTG 1 cut(s) 950
SacI GAGCTC 1 cut(s) 375
SapI GCTCTTC 1 cut(s) 1560
SaqAI TTAA 2 cut(s) 420, 1446
SatI GCNGC 2 cut(s) 234, 839
Sau3AI GATC 1 cut(s) 289
Sau96I GGNCC 3 cut(s) 924, 1211, 1308
SchI GAGTC 2 cut(s) 474, 968
ScrFI CCNGG 3 cut(s) 516, 1076, 1110
SduI GDGCHC 1 cut(s) 375
SfaNI GCATC 4 cut(s) 244, 305, 568, 1317
SinI GGWCC 2 cut(s) 924, 1308
SmiMI CAYNNNNRTG 1 cut(s) 950
SmlI CTYRAG 3 cut(s) 509, 1385, 1398
SmoI CTYRAG 3 cut(s) 509, 1385, 1398
SphI GCATGC 2 cut(s) 40, 955
SsiI CCGC 1 cut(s) 275
SspMI CTAG 1 cut(s) 162
SstI GAGCTC 1 cut(s) 375
StyD4I CCNGG 3 cut(s) 514, 1074, 1108
StyI CCWWGG 2 cut(s) 687, 1214
TaaI ACNGT 3 cut(s) 769, 1058, 1260
TaiI ACGT 1 cut(s) 388
TaqII GACCGA 1 cut(s) 941
TatI WGTACW 4 cut(s) 769, 1023, 1058, 1524
TfiI GAWTC 3 cut(s) 778, 1141, 1451
Tru1I TTAA 2 cut(s) 420, 1446
Tru9I TTAA 2 cut(s) 420, 1446
TscAI CASTG 4 cut(s) 352, 396, 772, 952
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 2 cut(s) 233, 838
Tsp45I GTSAC 1 cut(s) 464
TspDTI ATGAA 2 cut(s) 414, 722
TspGWI ACGGA 2 cut(s) 200, 284
TspRI CASTG 4 cut(s) 352, 396, 772, 952
Van91I CCANNNNNTGG 2 cut(s) 223, 1352
VpaK11BI GGWCC 2 cut(s) 924, 1308
XagI CCTNNNNNAGG 1 cut(s) 593
XapI RAATTY 3 cut(s) 815, 865, 918
XceI RCATGY 5 cut(s) 40, 721, 765, 955, 1394
XmiI GTMKAC 1 cut(s) 1255
XspI CTAG 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.