MD06G1082300.v1.1

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001519
Physical Location & Seq
Reverse (-)
20183516 .. 20185381
1866 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1082300.v1.1.491

Sequence Viewer

Length: 801 bp
ATGGCACTCGTTTCACAGACCGGTTCATCATCCTGCATTCACTTCTCTTCCGTACCGCCCTACACTGCTAGGAAGAAGAAGATTTCATATGCATACAATGCAGGAGTGGGGAAGATGAAACCGCGACCCAGTGTGATAGTTGCTGCAAGTCCTCCCGCAGAAGCTGCTGTAATAGCTACCGAGCCACTGACAAAAGAGGATCTTATAGGGCATCTTGCCTCTGGATGTAAGCCTAAGGAAAAATGGAGAATTGGCACTGAACAGGAGAGGTTTGGCTTTGATCTGAAAACTTTAAGTCCTATGACATACGAACACATTGCTAAGTTACTTAATGGGATTGCTGAGAGGTTTGATTGGGAAAAAATTATGGAAGGGGACAACATTATTGCACTTAAACAGGTTAAAGAAGTTCTAAACGAAATGGGAATCGGATTGATGGATATTGGTTGTCAGCCGAAGTGGGAAGTTAAAGACATACCTATGATGCCTAAGGAAAGATATGAGATCATTAGGAATTACATGCCCAAAGTTGGCTCTCTTGGACTTGATTTGATGTTCAGGACTTGCACTGCACAGGTTAATCTGGACTTTAGTTCTGAAGTGGGCATGATAAAGAAACTTCGCGCTAGTCTTGCTTTGCAGCCTGTGCGAACACTGAACTTGTTTTCGGTGTACATTTTTCTGCTTATATGGTATCTAGCTTTTCAACAAATGGAACAGATTGTAACAGCTCTATTTGCAAATTCACCTTTCACTGAAGGAAAGCCAAATGGTTTTCTTAGCATGAGACGGTTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

267

Amino Acids

29.92

Weight (kDa)

9.16

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 130 - 216 5e-20 Glutamate-cysteine ligase family 2(GCS2)
GCS2 PF04107 242 - 264 4.4e-06 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 124, 624
AciI CCGC 3 cut(s) 56, 122, 156
AclWI GGATC 1 cut(s) 207
AcsI RAATTY 1 cut(s) 742
AcuI CTGAAG 2 cut(s) 618, 777
AfaI GTAC 2 cut(s) 54, 674
AfiI CCNNNNNNNGG 1 cut(s) 530
AgeI ACCGGT 1 cut(s) 20
AgsI TTSAA 1 cut(s) 707
AluBI AGCT 4 cut(s) 164, 176, 701, 731
AluI AGCT 4 cut(s) 164, 176, 701, 731
Alw26I GTCTC 1 cut(s) 781
AlwI GGATC 1 cut(s) 207
AlwNI CAGNNNCTG 1 cut(s) 164
ApeKI GCWGC 3 cut(s) 143, 164, 640
ApoI RAATTY 1 cut(s) 742
ArsI GACNNNNNNTTYG 1 cut(s) 780
AsiGI ACCGGT 1 cut(s) 20
AspLEI GCGC 1 cut(s) 626
AsuHPI GGTGA 1 cut(s) 738
AxyI CCTNAGG 2 cut(s) 234, 489
BbvI GCAGC 3 cut(s) 130, 151, 652
BccI CCATC 1 cut(s) 430
BcgI CGANNNNNNTGC 2 cut(s) 299, 333
BcoDI GTCTC 1 cut(s) 781
BfaI CTAG 3 cut(s) 69, 627, 698
BisI GCNGC 3 cut(s) 144, 165, 641
BlsI GCNGC 3 cut(s) 145, 166, 642
BmrI ACTGGG 1 cut(s) 123
BmsI GCATC 2 cut(s) 220, 474
BmuI ACTGGG 1 cut(s) 123
BsaWI WCCGGW 1 cut(s) 20
Bsc4I CCNNNNNNNGG 1 cut(s) 530
Bse118I RCCGGY 1 cut(s) 20
Bse1I ACTGG 1 cut(s) 129
Bse21I CCTNAGG 2 cut(s) 234, 489
Bse3DI GCAATG 1 cut(s) 315
BseGI GGATG 2 cut(s) 29, 230
BseLI CCNNNNNNNGG 1 cut(s) 530
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 1 cut(s) 333
BseNI ACTGG 1 cut(s) 129
BseXI GCAGC 3 cut(s) 130, 151, 652
BsgI GTGCAG 1 cut(s) 555
Bsh1236I CGCG 2 cut(s) 124, 624
BshTI ACCGGT 1 cut(s) 20
BsiSI CCGG 1 cut(s) 21
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 1 cut(s) 530
BsmAI GTCTC 1 cut(s) 781
BsmBI CGTCTC 1 cut(s) 781
BsmFI GGGAC 1 cut(s) 389
BsmI GAATGC 1 cut(s) 36
Bsp1407I TGTACA 1 cut(s) 672
Bsp143I GATC 3 cut(s) 199, 280, 504
BspACI CCGC 3 cut(s) 56, 122, 156
BspCNI CTCAG 1 cut(s) 334
BspFNI CGCG 2 cut(s) 124, 624
BspPI GGATC 1 cut(s) 207
BsrDI GCAATG 1 cut(s) 315
BsrFI RCCGGY 1 cut(s) 20
BsrGI TGTACA 1 cut(s) 672
BsrI ACTGG 1 cut(s) 129
BssAI RCCGGY 1 cut(s) 20
BssMI GATC 3 cut(s) 199, 280, 504
Bst4CI ACNGT 1 cut(s) 792
Bst6I CTCTTC 1 cut(s) 52
BstAPI GCANNNNNTGC 3 cut(s) 98, 164, 646
BstAUI TGTACA 1 cut(s) 672
BstDEI CTNAG 5 cut(s) 234, 321, 342, 489, 779
BstF5I GGATG 2 cut(s) 29, 230
BstFNI CGCG 2 cut(s) 124, 624
BstHHI GCGC 1 cut(s) 626
BstKTI GATC 3 cut(s) 202, 283, 507
BstMAI GTCTC 1 cut(s) 781
BstMBI GATC 3 cut(s) 199, 280, 504
BstMWI GCNNNNNNNGC 6 cut(s) 98, 164, 173, 632, 646, 737
BstNSI RCATGY 1 cut(s) 523
BstUI CGCG 2 cut(s) 124, 624
BstV1I GCAGC 3 cut(s) 130, 151, 652
BstX2I RGATCY 1 cut(s) 199
BstYI RGATCY 1 cut(s) 199
Bsu36I CCTNAGG 2 cut(s) 234, 489
BtsCI GGATG 2 cut(s) 29, 230
BtsI GCAGTG 2 cut(s) 63, 567
BtsIMutI CAGTG 7 cut(s) 63, 136, 185, 255, 567, 653, 753
CaiI CAGNNNCTG 1 cut(s) 164
CfoI GCGC 1 cut(s) 626
Cfr10I RCCGGY 1 cut(s) 20
Csp6I GTAC 2 cut(s) 53, 673
CspAI ACCGGT 1 cut(s) 20
CviAII CATG 3 cut(s) 520, 607, 784
CviQI GTAC 2 cut(s) 53, 673
DdeI CTNAG 5 cut(s) 234, 321, 342, 489, 779
DpnI GATC 3 cut(s) 201, 282, 506
DpnII GATC 3 cut(s) 199, 280, 504
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
Eco57I CTGAAG 2 cut(s) 618, 777
Eco81I CCTNAGG 2 cut(s) 234, 489
EcoT22I ATGCAT 1 cut(s) 94
Esp3I CGTCTC 1 cut(s) 781
FaeI CATG 3 cut(s) 523, 610, 787
FalI AAGNNNNNCTT 2 cut(s) 186, 218
FaqI GGGAC 1 cut(s) 389
FatI CATG 3 cut(s) 519, 606, 783
FauI CCCGC 1 cut(s) 163
FauNDI CATATG 1 cut(s) 88
Fnu4HI GCNGC 3 cut(s) 144, 165, 641
FokI GGATG 2 cut(s) 16, 237
Fsp4HI GCNGC 3 cut(s) 144, 165, 641
FspBI CTAG 3 cut(s) 69, 627, 698
GlaI GCGC 1 cut(s) 625
GluI GCNGC 3 cut(s) 144, 165, 641
HapII CCGG 1 cut(s) 21
HhaI GCGC 1 cut(s) 626
Hin1II CATG 3 cut(s) 523, 610, 787
Hin6I GCGC 1 cut(s) 624
HinP1I GCGC 1 cut(s) 624
HinfI GANTC 1 cut(s) 426
HpaII CCGG 1 cut(s) 21
HphI GGTGA 1 cut(s) 738
Hpy166II GTNNAC 1 cut(s) 673
Hpy188I TCNGA 3 cut(s) 285, 431, 598
Hpy188III TCNNGA 3 cut(s) 222, 559, 584
Hpy8I GTNNAC 1 cut(s) 673
HpyAV CCTTC 2 cut(s) 365, 752
HpyCH4III ACNGT 1 cut(s) 792
HpyCH4V TGCA 9 cut(s) 36, 92, 101, 146, 389, 567, 572, 640, 740
HpyF10VI GCNNNNNNNGC 6 cut(s) 98, 164, 173, 632, 646, 737
HpyF3I CTNAG 5 cut(s) 234, 321, 342, 489, 779
Hsp92II CATG 3 cut(s) 523, 610, 787
HspAI GCGC 1 cut(s) 624
Kzo9I GATC 3 cut(s) 199, 280, 504
Lsp1109I GCAGC 3 cut(s) 130, 151, 652
LweI GCATC 2 cut(s) 220, 474
MaeI CTAG 3 cut(s) 69, 627, 698
MaeIII GTNAC 2 cut(s) 324, 724
MalI GATC 3 cut(s) 201, 282, 506
MboI GATC 3 cut(s) 199, 280, 504
MboII GAAGA 5 cut(s) 39, 85, 88, 91, 124
MflI RGATCY 1 cut(s) 199
MluCI AATT 4 cut(s) 249, 363, 514, 742
MnlI CCTC 5 cut(s) 162, 190, 229, 261, 339
Mph1103I ATGCAT 1 cut(s) 94
MseI TTAA 6 cut(s) 293, 330, 393, 402, 468, 579
MslI CAYNNNNRTG 1 cut(s) 479
MspI CCGG 1 cut(s) 21
Mva1269I GAATGC 1 cut(s) 36
MvnI CGCG 2 cut(s) 124, 624
MwoI GCNNNNNNNGC 6 cut(s) 98, 164, 173, 632, 646, 737
NdeI CATATG 1 cut(s) 88
NdeII GATC 3 cut(s) 199, 280, 504
NlaIII CATG 3 cut(s) 523, 610, 787
NsiI ATGCAT 1 cut(s) 94
NspI RCATGY 1 cut(s) 523
PctI GAATGC 1 cut(s) 36
PfeI GAWTC 1 cut(s) 426
PinAI ACCGGT 1 cut(s) 20
PkrI GCNGC 3 cut(s) 145, 166, 642
PstNI CAGNNNCTG 1 cut(s) 164
PsuI RGATCY 1 cut(s) 199
RsaI GTAC 2 cut(s) 54, 674
RsaNI GTAC 2 cut(s) 53, 673
RseI CAYNNNNRTG 1 cut(s) 479
SaqAI TTAA 6 cut(s) 293, 330, 393, 402, 468, 579
SatI GCNGC 3 cut(s) 144, 165, 641
Sau3AI GATC 3 cut(s) 199, 280, 504
SfaNI GCATC 2 cut(s) 220, 474
SmiMI CAYNNNNRTG 1 cut(s) 479
Sse9I AATT 4 cut(s) 249, 363, 514, 742
SsiI CCGC 3 cut(s) 56, 122, 156
SspMI CTAG 3 cut(s) 69, 627, 698
TaaI ACNGT 1 cut(s) 792
TasI AATT 4 cut(s) 249, 363, 514, 742
TatI WGTACW 1 cut(s) 672
TfiI GAWTC 1 cut(s) 426
Tru1I TTAA 6 cut(s) 293, 330, 393, 402, 468, 579
Tru9I TTAA 6 cut(s) 293, 330, 393, 402, 468, 579
TscAI CASTG 7 cut(s) 70, 136, 192, 262, 574, 660, 760
TseI GCWGC 3 cut(s) 143, 164, 640
TspDTI ATGAA 3 cut(s) 15, 75, 131
TspGWI ACGGA 1 cut(s) 40
TspRI CASTG 7 cut(s) 70, 136, 192, 262, 574, 660, 760
XapI RAATTY 1 cut(s) 742
XceI RCATGY 1 cut(s) 523
XspI CTAG 3 cut(s) 69, 627, 698
Zsp2I ATGCAT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.