Rmu_sc0006732.1_g000017

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006732.1
OG0001519
Physical Location & Seq
Forward (+)
95937 .. 96481
545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006732.1_g000017.1.cds

Sequence Viewer

Length: 291 bp
atgatagctgattggactcctgaagaaagacagatgctgagagataaggttccgaaaacgggtctaaagacaccatttcgtgatggattactcaagcaagttgccgaagatgtagtaaagtttgcaaaggatggcctggaaagaagaggctttaaggaatcagggttcttaaatgaagcggtggagattgtgagaataggtgtaacaccatctgagaagctattggagttgtataatgggaagtggaaacaacagatagacccagtttttgaagagctactatactcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

96

Amino Acids

11.11

Weight (kDa)

5.54

Isoelectric Point (pI)

35.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 179
AcuI CTGAAG 1 cut(s) 42
AfiI CCNNNNNNNGG 1 cut(s) 59
AgsI TTSAA 1 cut(s) 272
AjnI CCWGG 1 cut(s) 135
AloI GAACNNNNNNTCC 2 cut(s) 149, 181
AluBI AGCT 3 cut(s) 8, 220, 277
AluI AGCT 3 cut(s) 8, 220, 277
AlwNI CAGNNNCTG 1 cut(s) 37
AoxI GGCC 1 cut(s) 133
ArsI GACNNNNNNTTYG 2 cut(s) 251, 283
BccI CCATC 3 cut(s) 77, 125, 217
BciT130I CCWGG 1 cut(s) 137
Bme1390I CCNGG 1 cut(s) 137
BmiI GGNNCC 1 cut(s) 51
BmrFI CCNGG 1 cut(s) 137
BmrI ACTGGG 1 cut(s) 257
BmsI GCATC 1 cut(s) 24
BmuI ACTGGG 1 cut(s) 257
BpuEI CTTGAG 1 cut(s) 77
Bsc4I CCNNNNNNNGG 1 cut(s) 59
Bse1I ACTGG 1 cut(s) 263
BseBI CCWGG 1 cut(s) 137
BseGI GGATG 1 cut(s) 136
BseLI CCNNNNNNNGG 1 cut(s) 59
BseMII CTCAG 2 cut(s) 29, 204
BseNI ACTGG 1 cut(s) 263
BshFI GGCC 1 cut(s) 135
BslI CCNNNNNNNGG 1 cut(s) 59
BsnI GGCC 1 cut(s) 135
BspACI CCGC 1 cut(s) 179
BspANI GGCC 1 cut(s) 135
BspCNI CTCAG 2 cut(s) 30, 205
BspHI TCATGA 1 cut(s) 287
BspLI GGNNCC 1 cut(s) 51
BspQI GCTCTTC 1 cut(s) 267
BsrI ACTGG 1 cut(s) 263
Bst2UI CCWGG 1 cut(s) 137
Bst6I CTCTTC 2 cut(s) 139, 267
BstDEI CTNAG 2 cut(s) 38, 213
BstF5I GGATG 1 cut(s) 136
BstNI CCWGG 1 cut(s) 137
BstSCI CCNGG 1 cut(s) 135
BsuRI GGCC 1 cut(s) 135
BtsCI GGATG 1 cut(s) 136
CaiI CAGNNNCTG 1 cut(s) 37
CciI TCATGA 1 cut(s) 287
CviAII CATG 1 cut(s) 288
CviJI RGCY 5 cut(s) 8, 135, 150, 220, 277
CviKI_1 RGCY 5 cut(s) 8, 135, 150, 220, 277
DdeI CTNAG 2 cut(s) 38, 213
Eam1104I CTCTTC 2 cut(s) 139, 267
EarI CTCTTC 2 cut(s) 139, 267
Eco57I CTGAAG 1 cut(s) 42
EcoRII CCWGG 1 cut(s) 135
FaeI CATG 1 cut(s) 291
FaiI YATR 3 cut(s) 234, 283, 289
FatI CATG 1 cut(s) 287
FokI GGATG 1 cut(s) 143
HaeIII GGCC 1 cut(s) 135
Hin1II CATG 1 cut(s) 291
HinfI GANTC 2 cut(s) 16, 158
Hpy188I TCNGA 2 cut(s) 54, 214
Hpy188III TCNNGA 3 cut(s) 20, 80, 288
HpyCH4V TGCA 1 cut(s) 125
HpyF3I CTNAG 2 cut(s) 38, 213
Hsp92II CATG 1 cut(s) 291
LguI GCTCTTC 1 cut(s) 267
LpnPI CCDG 5 cut(s) 33, 122, 147, 149, 276
LweI GCATC 1 cut(s) 24
MaeIII GTNAC 1 cut(s) 202
MboII GAAGA 4 cut(s) 35, 119, 156, 284
MlyI GAGTC 1 cut(s) 10
MnlI CCTC 1 cut(s) 140
MseI TTAA 2 cut(s) 153, 170
MspR9I CCNGG 1 cut(s) 137
MvaI CCWGG 1 cut(s) 137
NlaIII CATG 1 cut(s) 291
NlaIV GGNNCC 1 cut(s) 51
PagI TCATGA 1 cut(s) 287
PciSI GCTCTTC 1 cut(s) 267
PfeI GAWTC 1 cut(s) 158
PleI GAGTC 1 cut(s) 10
PpsI GAGTC 1 cut(s) 10
Psp6I CCWGG 1 cut(s) 135
PspGI CCWGG 1 cut(s) 135
PspN4I GGNNCC 1 cut(s) 51
PstNI CAGNNNCTG 1 cut(s) 37
SapI GCTCTTC 1 cut(s) 267
SaqAI TTAA 2 cut(s) 153, 170
SchI GAGTC 1 cut(s) 10
ScrFI CCNGG 1 cut(s) 137
SetI ASST 5 cut(s) 10, 51, 202, 222, 279
SfaNI GCATC 1 cut(s) 24
SgeI CNNG 9 cut(s) 32, 72, 92, 106, 110, 148, 149, 174, 275
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
SsiI CCGC 1 cut(s) 179
StyD4I CCNGG 1 cut(s) 135
TfiI GAWTC 1 cut(s) 158
Tru1I TTAA 2 cut(s) 153, 170
Tru9I TTAA 2 cut(s) 153, 170
TspDTI ATGAA 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.