Rmu_sc0002343.1_g000010

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002343.1
OG0001519
Physical Location & Seq
Reverse (-)
62917 .. 63764
848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002343.1_g000010.1.cds

Sequence Viewer

Length: 465 bp
atggtgtatacaagggggtcggaggagagaacgagagtggtggtggctgccttccgggtgaatatggcaggaaagcttcactgccttccgggtgaattaccgaccctgaatgattgggagaatcatttgggaactctatatccagaggtcaggctgaagagatacttagagatgaggggggctgatggagggccttggaggcggttatgtgctttgcctgcattttgggtgggtttactgtatgatgaggtttccctgcagaatgtgttagacatgatagcttattggactcctaaagaaagacagatgctgagagataagctgctgtcagtttgtgtctttgtgtattcaggcattggagagaacgagagtggtggtgctgggatcggagttcaagatttggctgctgtgattcaccgtggccgtaatcgcttaaggcggcgccggtgttcttcaaggccatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

154

Amino Acids

17.55

Weight (kDa)

9.58

Isoelectric Point (pI)

41.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 441
AccI GTMKAC 1 cut(s) 8
AciI CCGC 2 cut(s) 202, 439
AclWI GGATC 1 cut(s) 392
AcoI YGGCCR 1 cut(s) 421
AcuI CTGAAG 1 cut(s) 176
AcyI GRCGYC 1 cut(s) 442
AflII CTTAAG 1 cut(s) 433
AgsI TTSAA 2 cut(s) 395, 456
AloI GAACNNNNNNTCC 2 cut(s) 124, 156
AluBI AGCT 3 cut(s) 76, 281, 322
AluI AGCT 3 cut(s) 76, 281, 322
AlwI GGATC 1 cut(s) 392
AlwNI CAGNNNCTG 1 cut(s) 310
AoxI GGCC 3 cut(s) 191, 421, 458
ApeKI GCWGC 3 cut(s) 47, 322, 404
AspLEI GCGC 1 cut(s) 444
AspS9I GGNCC 1 cut(s) 191
AsuC2I CCSGG 2 cut(s) 56, 90
AsuHPI GGTGA 3 cut(s) 70, 104, 407
BanI GGYRCC 1 cut(s) 441
BbvI GCAGC 3 cut(s) 34, 309, 391
BccI CCATC 1 cut(s) 179
BceAI ACGGC 1 cut(s) 408
BcnI CCSGG 2 cut(s) 56, 90
BfmI CTRYAG 1 cut(s) 257
BfoI RGCGCY 1 cut(s) 445
BfrI CTTAAG 1 cut(s) 433
BglI GCCNNNNNGGC 1 cut(s) 199
BisI GCNGC 4 cut(s) 48, 323, 405, 440
BlsI GCNGC 4 cut(s) 49, 324, 406, 441
Bme1390I CCNGG 2 cut(s) 56, 90
BmgT120I GGNCC 1 cut(s) 191
BmiI GGNNCC 1 cut(s) 443
BmrFI CCNGG 2 cut(s) 56, 90
BmsI GCATC 1 cut(s) 297
BpuMI CCSGG 2 cut(s) 56, 90
BsaHI GRCGYC 1 cut(s) 442
BsaJI CCNNGG 2 cut(s) 194, 418
Bse118I RCCGGY 1 cut(s) 444
BseDI CCNNGG 2 cut(s) 194, 418
BseMII CTCAG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 38
BseXI GCAGC 3 cut(s) 34, 309, 391
BseYI CCCAGC 1 cut(s) 380
BshFI GGCC 3 cut(s) 193, 423, 460
BshNI GGYRCC 1 cut(s) 441
BsiSI CCGG 3 cut(s) 55, 89, 445
BsnI GGCC 3 cut(s) 193, 423, 460
Bsp143I GATC 1 cut(s) 384
BspACI CCGC 2 cut(s) 202, 439
BspANI GGCC 3 cut(s) 193, 423, 460
BspCNI CTCAG 1 cut(s) 303
BspLI GGNNCC 1 cut(s) 443
BspMAI CTGCAG 1 cut(s) 261
BspPI GGATC 1 cut(s) 392
BspT107I GGYRCC 1 cut(s) 441
BspTI CTTAAG 1 cut(s) 433
BsrFI RCCGGY 1 cut(s) 444
BssAI RCCGGY 1 cut(s) 444
BssECI CCNNGG 2 cut(s) 194, 418
BssMI GATC 1 cut(s) 384
BssNAI GTATAC 1 cut(s) 9
BssNI GRCGYC 1 cut(s) 442
BssT1I CCWWGG 1 cut(s) 194
Bst1107I GTATAC 1 cut(s) 9
Bst4CI ACNGT 2 cut(s) 240, 419
Bst6I CTCTTC 1 cut(s) 152
BstACI GRCGYC 1 cut(s) 442
BstAFI CTTAAG 1 cut(s) 433
BstC8I GCNNGC 1 cut(s) 219
BstDEI CTNAG 2 cut(s) 166, 311
BstDSI CCRYGG 1 cut(s) 418
BstH2I RGCGCY 1 cut(s) 445
BstHHI GCGC 1 cut(s) 444
BstKTI GATC 1 cut(s) 387
BstMBI GATC 1 cut(s) 384
BstMWI GCNNNNNNNGC 3 cut(s) 199, 218, 429
BstSCI CCNGG 2 cut(s) 54, 88
BstSFI CTRYAG 1 cut(s) 257
BstV1I GCAGC 3 cut(s) 34, 309, 391
BstZ17I GTATAC 1 cut(s) 9
BsuRI GGCC 3 cut(s) 193, 423, 460
BtgI CCRYGG 1 cut(s) 418
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 1 cut(s) 79
Cac8I GCNNGC 1 cut(s) 219
CaiI CAGNNNCTG 1 cut(s) 310
CfoI GCGC 1 cut(s) 444
Cfr10I RCCGGY 1 cut(s) 444
Cfr13I GGNCC 1 cut(s) 191
CviAII CATG 2 cut(s) 274, 462
DdeI CTNAG 2 cut(s) 166, 311
DinI GGCGCC 1 cut(s) 443
DpnI GATC 1 cut(s) 386
DpnII GATC 1 cut(s) 384
EaeI YGGCCR 1 cut(s) 421
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 194
Eco57I CTGAAG 1 cut(s) 176
EcoO109I RGGNCCY 1 cut(s) 191
EcoT14I CCWWGG 1 cut(s) 194
EgeI GGCGCC 1 cut(s) 443
EheI GGCGCC 1 cut(s) 443
ErhI CCWWGG 1 cut(s) 194
FaeI CATG 2 cut(s) 277, 465
FaiI YATR 7 cut(s) 9, 65, 139, 208, 243, 275, 463
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FatI CATG 2 cut(s) 273, 461
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 4 cut(s) 48, 323, 405, 440
Fsp4HI GCNGC 4 cut(s) 48, 323, 405, 440
GlaI GCGC 1 cut(s) 443
GluI GCNGC 4 cut(s) 48, 323, 405, 440
GsaI CCCAGC 1 cut(s) 384
HaeII RGCGCY 1 cut(s) 445
HaeIII GGCC 3 cut(s) 193, 423, 460
HapII CCGG 3 cut(s) 55, 89, 445
HhaI GCGC 1 cut(s) 444
Hin1I GRCGYC 1 cut(s) 442
Hin1II CATG 2 cut(s) 277, 465
Hin6I GCGC 1 cut(s) 442
HinP1I GCGC 1 cut(s) 442
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 3 cut(s) 121, 289, 412
HpaII CCGG 3 cut(s) 55, 89, 445
HphI GGTGA 3 cut(s) 70, 104, 407
Hpy166II GTNNAC 2 cut(s) 9, 236
Hpy188I TCNGA 2 cut(s) 22, 389
Hpy188III TCNNGA 2 cut(s) 143, 395
Hpy8I GTNNAC 2 cut(s) 9, 236
HpyAV CCTTC 2 cut(s) 61, 95
HpyCH4III ACNGT 2 cut(s) 240, 419
HpyCH4V TGCA 2 cut(s) 221, 259
HpyF10VI GCNNNNNNNGC 3 cut(s) 199, 218, 429
HpyF3I CTNAG 2 cut(s) 166, 311
Hsp92I GRCGYC 1 cut(s) 442
Hsp92II CATG 2 cut(s) 277, 465
HspAI GCGC 1 cut(s) 442
KasI GGCGCC 1 cut(s) 441
Kzo9I GATC 1 cut(s) 384
Lsp1109I GCAGC 3 cut(s) 34, 309, 391
LweI GCATC 1 cut(s) 297
MalI GATC 1 cut(s) 386
MboI GATC 1 cut(s) 384
MboII GAAGA 2 cut(s) 169, 444
MluCI AATT 1 cut(s) 95
Mly113I GGCGCC 1 cut(s) 442
MlyI GAGTC 1 cut(s) 283
MnlI CCTC 6 cut(s) 16, 139, 168, 182, 192, 241
MseI TTAA 1 cut(s) 434
MspCI CTTAAG 1 cut(s) 433
MspI CCGG 3 cut(s) 55, 89, 445
MspR9I CCNGG 2 cut(s) 56, 90
MwoI GCNNNNNNNGC 3 cut(s) 199, 218, 429
NarI GGCGCC 1 cut(s) 442
NciI CCSGG 2 cut(s) 56, 90
NdeII GATC 1 cut(s) 384
NlaIII CATG 2 cut(s) 277, 465
NlaIV GGNNCC 1 cut(s) 443
PfeI GAWTC 2 cut(s) 121, 412
PkrI GCNGC 4 cut(s) 49, 324, 406, 441
PleI GAGTC 1 cut(s) 283
PluTI GGCGCC 1 cut(s) 445
PpsI GAGTC 1 cut(s) 283
PspFI CCCAGC 1 cut(s) 380
PspN4I GGNNCC 1 cut(s) 443
PspPI GGNCC 1 cut(s) 191
PstI CTGCAG 1 cut(s) 261
PstNI CAGNNNCTG 1 cut(s) 310
SaqAI TTAA 1 cut(s) 434
SatI GCNGC 4 cut(s) 48, 323, 405, 440
Sau3AI GATC 1 cut(s) 384
Sau96I GGNCC 1 cut(s) 191
SchI GAGTC 1 cut(s) 283
ScrFI CCNGG 2 cut(s) 56, 90
SetI ASST 5 cut(s) 78, 150, 252, 283, 324
SfaNI GCATC 1 cut(s) 297
SfcI CTRYAG 1 cut(s) 257
SfoI GGCGCC 1 cut(s) 443
SgrAI CRCCGGYG 1 cut(s) 444
SmlI CTYRAG 1 cut(s) 433
SmoI CTYRAG 1 cut(s) 433
Sse9I AATT 1 cut(s) 95
SsiI CCGC 2 cut(s) 202, 439
SspDI GGCGCC 1 cut(s) 441
StyD4I CCNGG 2 cut(s) 54, 88
StyI CCWWGG 1 cut(s) 194
TaaI ACNGT 2 cut(s) 240, 419
TasI AATT 1 cut(s) 95
TauI GCSGC 1 cut(s) 442
TfiI GAWTC 2 cut(s) 121, 412
Tru1I TTAA 1 cut(s) 434
Tru9I TTAA 1 cut(s) 434
TscAI CASTG 1 cut(s) 86
TseI GCWGC 3 cut(s) 47, 322, 404
TspRI CASTG 1 cut(s) 86
Vha464I CTTAAG 1 cut(s) 433
XmiI GTMKAC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.