Rh7AG167400

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001519
Physical Location & Seq
Forward (+)
14308889 .. 14312480
3592 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG167400.1

Sequence Viewer

Length: 1506 bp
ATGACACTCATTTCCCAGACAAGTTCATCATACTACTGCAGAAGCATTGGCTTCTCTTCCTCTGCATTTTATTCTACTACTACTACTAGGAAGATACAGGTTTTGCATGTAGAAAATGTTGCAGGACAGAAACGAGGACGTAAGAGTGTGATAGTTGCTGCAAGTCCTATCACAGAAGATGCTGTAGTAGCTACAAAGCCGCTGATGAAAGAAGATCTTGTAGGCTATCTTGCCTCTGGATGCAAGCCCAAAGAAAAATGGAGAATTGGCATGGAACACGAGAGGTTTGGCTTCGAACTAAATACTTTACGTCCGATGCAATATGAACAAATAGCTGAGCTGCTCTATGGGATTGCTGGGAGAATTGAGTGGGAGAAAATCATGGAAGGTGGTAAGATTATTGGACTCAAACAGGGTAAGCAGAGCATATCACTAGAGCCAGGAGGTCAATTTGAGCTTAGTGGTGCACCTCTTGAAACTCTGCATCAAATTTGTATTGAAGTCAATTCACAACTCTGTCAGGTCAAAGCAGTTGCAGAGAAAATGGGAATCGGATTTCTTGAAATTGGTGGTCAGCCGAAATGGAGAATTCAAGACATACCTATGATGCCCAAGGGAAGATATGAGATTATTAGAAATTACATGCCAAAAGTTGGCTCGCTTGGACATGATTTGATGTTCAGAACTTGCACTGTACAGGTCAATCTGGACTTCAGTTCTGAAGCAGACATGAAACGGAAATTGCGTGCTAGTCTTGCTTTGCAGCCTATAGCAACAGCCCTATTTGCAAATTCACCTTTCACTGAAGGAAAACCGAATGGTTTTCTAAGCAGGAGAAGTCACATCTATACTGATACTGACAAGGATCGCACTGGCATGCTGCCTTTTGCTTTTGATGACTCCTTTGGGTTTGAGCGGTATGTTGATTATGCTCTTGATGTTCCCATGTACTGTGTTTATCGGAAAAATAAGTGTATCGACTGTACTGGAACGACCTTTCGGGACTTTATGGCGGGAAAGCTTCGTTGCCTTCCAGGTGAATTACCAACACTGAATGATTGGGAGAATCATTTGGGAACTATTTATCCAGAGGTCAGGCTGAAGAGATTCTTGGAGATGAGGGGTGCTGATGGAGGGCCTTGGAGAAGCTTATGCGCTTTGCCTGCATTTTGGGTGGGTCTACTATATGATGAAGTTTCCCTGCAGAATGTGTTAGACATGATTGCTGATTGGACTCCTGAAGAAAGACAGATGCTGAGAGATAAGATTCCAAAAACGGGTCTAAAGACACCATTTCGTGAAGGAATAGTCAAGCATGTTGCCGACGACGTAGTAAAGTTTGCAAAGGATGGCCTGGAAAGAAGAGGCTTTAAGGAATCAGGGTTTTTAAATGAGGTGGTGGAGGTTGTGAGAACTGGTGTAAAACCAGCCGAGAAGCTATTGGAATTATATAATGGGAAATGGAAACAACAGATAGACCCAGTTTTTGAAGAGCTACTATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

501

Amino Acids

56.77

Weight (kDa)

8.36

Isoelectric Point (pI)

37.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 88 - 498 5.4e-97 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 653
AccBSI CCGCTC 1 cut(s) 916
AccI GTMKAC 1 cut(s) 1180
AciI CCGC 3 cut(s) 200, 916, 1013
AclWI GGATC 1 cut(s) 873
AcsI RAATTY 3 cut(s) 489, 588, 790
AcuI CTGAAG 5 cut(s) 697, 741, 825, 1121, 1260
AfaI GTAC 3 cut(s) 696, 950, 985
AfiI CCNNNNNNNGG 2 cut(s) 653, 1277
AgsI TTSAA 5 cut(s) 476, 500, 563, 593, 1490
AjnI CCWGG 3 cut(s) 439, 1033, 1353
AjuI GAANNNNNNNTTGG 2 cut(s) 1094, 1126
AloI GAACNNNNNNTCC 2 cut(s) 1069, 1101
AluBI AGCT 8 cut(s) 191, 335, 340, 457, 1021, 1149, 1438, 1495
AluI AGCT 8 cut(s) 191, 335, 340, 457, 1021, 1149, 1438, 1495
Alw21I GWGCWC 1 cut(s) 469
Alw44I GTGCAC 1 cut(s) 465
AlwI GGATC 1 cut(s) 873
AlwNI CAGNNNCTG 1 cut(s) 1255
AoxI GGCC 2 cut(s) 1136, 1351
ApaLI GTGCAC 1 cut(s) 465
ApeKI GCWGC 4 cut(s) 158, 340, 763, 880
ApoI RAATTY 3 cut(s) 489, 588, 790
ArsI GACNNNNNNTTYG 2 cut(s) 1469, 1501
Asp700I GAANNNNTTC 1 cut(s) 1106
AspLEI GCGC 1 cut(s) 1157
AspS9I GGNCC 1 cut(s) 1136
AsuHPI GGTGA 2 cut(s) 786, 1049
AsuII TTCGAA 1 cut(s) 294
BaeGI GKGCMC 1 cut(s) 469
BauI CACGAG 1 cut(s) 278
Bbv12I GWGCWC 1 cut(s) 469
BbvI GCAGC 4 cut(s) 145, 327, 775, 867
BccI CCATC 2 cut(s) 1124, 1343
BciT130I CCWGG 3 cut(s) 441, 1035, 1355
BfaI CTAG 3 cut(s) 87, 434, 750
BfmI CTRYAG 4 cut(s) 37, 183, 768, 1202
BglII AGATCT 1 cut(s) 214
BisI GCNGC 5 cut(s) 159, 200, 341, 764, 881
BlpI GCTNAGC 1 cut(s) 336
BlsI GCNGC 5 cut(s) 160, 201, 342, 765, 882
Bme1390I CCNGG 3 cut(s) 441, 1035, 1355
BmgT120I GGNCC 1 cut(s) 1136
BmrFI CCNGG 3 cut(s) 441, 1035, 1355
BmrI ACTGGG 1 cut(s) 1475
BmsI GCATC 6 cut(s) 169, 230, 306, 493, 597, 1242
BmuI ACTGGG 1 cut(s) 1475
Bpu1102I GCTNAGC 1 cut(s) 336
Bpu14I TTCGAA 1 cut(s) 294
BsaJI CCNNGG 2 cut(s) 612, 1139
Bsc4I CCNNNNNNNGG 2 cut(s) 653, 1277
Bse1I ACTGG 4 cut(s) 877, 991, 1420, 1481
BseBI CCWGG 3 cut(s) 441, 1035, 1355
BseDI CCNNGG 2 cut(s) 612, 1139
BseGI GGATG 2 cut(s) 245, 1354
BseLI CCNNNNNNNGG 2 cut(s) 653, 1277
BseMII CTCAG 2 cut(s) 327, 1247
BseNI ACTGG 4 cut(s) 877, 991, 1420, 1481
BseSI GKGCMC 1 cut(s) 469
BseXI GCAGC 4 cut(s) 145, 327, 775, 867
BseYI CCCAGC 1 cut(s) 356
BshFI GGCC 2 cut(s) 1138, 1353
BsiHKAI GWGCWC 1 cut(s) 469
BslFI GGGAC 1 cut(s) 1016
BslI CCNNNNNNNGG 2 cut(s) 653, 1277
BsmFI GGGAC 1 cut(s) 1016
BsnI GGCC 2 cut(s) 1138, 1353
Bsp119I TTCGAA 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 469
Bsp1407I TGTACA 1 cut(s) 694
Bsp143I GATC 2 cut(s) 214, 865
Bsp1720I GCTNAGC 1 cut(s) 336
BspACI CCGC 3 cut(s) 200, 916, 1013
BspANI GGCC 2 cut(s) 1138, 1353
BspCNI CTCAG 2 cut(s) 328, 1248
BspMAI CTGCAG 2 cut(s) 41, 1206
BspPI GGATC 1 cut(s) 873
BspQI GCTCTTC 1 cut(s) 1485
BspT104I TTCGAA 1 cut(s) 294
BsrBI CCGCTC 1 cut(s) 916
BsrGI TGTACA 1 cut(s) 694
BsrI ACTGG 4 cut(s) 877, 991, 1420, 1481
BssECI CCNNGG 2 cut(s) 612, 1139
BssMI GATC 2 cut(s) 214, 865
BssSI CACGAG 1 cut(s) 278
BssT1I CCWWGG 2 cut(s) 612, 1139
Bst2BI CACGAG 1 cut(s) 278
Bst2UI CCWGG 3 cut(s) 441, 1035, 1355
Bst4CI ACNGT 3 cut(s) 694, 953, 983
Bst6I CTCTTC 4 cut(s) 61, 1097, 1357, 1485
BstAUI TGTACA 1 cut(s) 694
BstBI TTCGAA 1 cut(s) 294
BstC8I GCNNGC 5 cut(s) 245, 659, 747, 878, 1164
BstDEI CTNAG 4 cut(s) 336, 458, 827, 1256
BstF5I GGATG 2 cut(s) 245, 1354
BstHHI GCGC 1 cut(s) 1157
BstKTI GATC 2 cut(s) 217, 868
BstMBI GATC 2 cut(s) 214, 865
BstMWI GCNNNNNNNGC 4 cut(s) 188, 755, 785, 1163
BstNI CCWGG 3 cut(s) 441, 1035, 1355
BstNSI RCATGY 4 cut(s) 110, 646, 880, 1319
BstSCI CCNGG 3 cut(s) 439, 1033, 1353
BstSFI CTRYAG 4 cut(s) 37, 183, 768, 1202
BstSLI GKGCMC 1 cut(s) 469
BstV1I GCAGC 4 cut(s) 145, 327, 775, 867
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
BsuRI GGCC 2 cut(s) 1138, 1353
BtsCI GGATG 2 cut(s) 245, 1354
BtsIMutI CAGTG 4 cut(s) 690, 801, 870, 1049
Cac8I GCNNGC 5 cut(s) 245, 659, 747, 878, 1164
CaiI CAGNNNCTG 1 cut(s) 1255
CfoI GCGC 1 cut(s) 1157
Cfr13I GGNCC 1 cut(s) 1136
Csp6I GTAC 3 cut(s) 695, 949, 984
CviQI GTAC 3 cut(s) 695, 949, 984
DdeI CTNAG 4 cut(s) 336, 458, 827, 1256
DpnI GATC 2 cut(s) 216, 867
DpnII GATC 2 cut(s) 214, 865
DraI TTTAAA 1 cut(s) 1389
Eam1104I CTCTTC 4 cut(s) 61, 1097, 1357, 1485
EarI CTCTTC 4 cut(s) 61, 1097, 1357, 1485
Eco130I CCWWGG 2 cut(s) 612, 1139
Eco57I CTGAAG 5 cut(s) 697, 741, 825, 1121, 1260
EcoO109I RGGNCCY 1 cut(s) 1136
EcoRI GAATTC 1 cut(s) 588
EcoRII CCWGG 3 cut(s) 439, 1033, 1353
EcoT14I CCWWGG 2 cut(s) 612, 1139
ErhI CCWWGG 2 cut(s) 612, 1139
FalI AAGNNNNNCTT 4 cut(s) 201, 233, 1094, 1126
FaqI GGGAC 1 cut(s) 1016
FauI CCCGC 1 cut(s) 1006
FblI GTMKAC 1 cut(s) 1180
Fnu4HI GCNGC 5 cut(s) 159, 200, 341, 764, 881
FokI GGATG 2 cut(s) 252, 1361
Fsp4HI GCNGC 5 cut(s) 159, 200, 341, 764, 881
FspBI CTAG 3 cut(s) 87, 434, 750
GlaI GCGC 1 cut(s) 1156
GluI GCNGC 5 cut(s) 159, 200, 341, 764, 881
GsaI CCCAGC 1 cut(s) 360
HaeIII GGCC 2 cut(s) 1138, 1353
HhaI GCGC 1 cut(s) 1157
Hin6I GCGC 1 cut(s) 1155
HinP1I GCGC 1 cut(s) 1155
HindIII AAGCTT 2 cut(s) 1019, 1147
HinfI GANTC 8 cut(s) 405, 549, 899, 1066, 1107, 1234, 1267, 1376
HphI GGTGA 2 cut(s) 786, 1049
Hpy166II GTNNAC 2 cut(s) 467, 1181
Hpy188I TCNGA 5 cut(s) 315, 554, 683, 721, 963
Hpy8I GTNNAC 2 cut(s) 467, 1181
Hpy99I CGWCG 2 cut(s) 1328, 1331
HpyAV CCTTC 4 cut(s) 380, 800, 1040, 1295
HpyCH4III ACNGT 3 cut(s) 694, 953, 983
HpyCH4IV ACGT 3 cut(s) 139, 310, 1329
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 755, 785, 1163
HpyF3I CTNAG 4 cut(s) 336, 458, 827, 1256
HpySE526I ACGT 3 cut(s) 139, 310, 1329
HspAI GCGC 1 cut(s) 1155
Kzo9I GATC 2 cut(s) 214, 865
LguI GCTCTTC 1 cut(s) 1485
Lsp1109I GCAGC 4 cut(s) 145, 327, 775, 867
LweI GCATC 6 cut(s) 169, 230, 306, 493, 597, 1242
MaeI CTAG 3 cut(s) 87, 434, 750
MaeII ACGT 3 cut(s) 139, 310, 1329
MaeIII GTNAC 1 cut(s) 839
MalI GATC 2 cut(s) 216, 867
MbiI CCGCTC 1 cut(s) 916
MboI GATC 2 cut(s) 214, 865
MboII GAAGA 9 cut(s) 48, 103, 188, 224, 630, 1114, 1253, 1374, 1502
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 1 cut(s) 469
MlyI GAGTC 3 cut(s) 399, 893, 1228
MroXI GAANNNNTTC 1 cut(s) 1106
MseI TTAA 2 cut(s) 1371, 1388
MslI CAYNNNNRTG 2 cut(s) 602, 875
MspA1I CMGCKG 1 cut(s) 202
MspR9I CCNGG 3 cut(s) 441, 1035, 1355
MvaI CCWGG 3 cut(s) 441, 1035, 1355
MwoI GCNNNNNNNGC 4 cut(s) 188, 755, 785, 1163
NdeII GATC 2 cut(s) 214, 865
NmeAIII GCCGAG 1 cut(s) 1456
NmuCI GTSAC 1 cut(s) 839
NspI RCATGY 4 cut(s) 110, 646, 880, 1319
NspV TTCGAA 1 cut(s) 294
PaeI GCATGC 1 cut(s) 880
PciSI GCTCTTC 1 cut(s) 1485
PcsI WCGNNNNNNNCGW 1 cut(s) 742
PdmI GAANNNNTTC 1 cut(s) 1106
PfeI GAWTC 5 cut(s) 549, 1066, 1107, 1267, 1376
PflMI CCANNNNNTGG 1 cut(s) 653
PkrI GCNGC 5 cut(s) 160, 201, 342, 765, 882
PleI GAGTC 3 cut(s) 399, 893, 1228
PpsI GAGTC 3 cut(s) 399, 893, 1228
Psp6I CCWGG 3 cut(s) 439, 1033, 1353
PspFI CCCAGC 1 cut(s) 356
PspGI CCWGG 3 cut(s) 439, 1033, 1353
PspPI GGNCC 1 cut(s) 1136
PstI CTGCAG 2 cut(s) 41, 1206
PstNI CAGNNNCTG 1 cut(s) 1255
PsuI RGATCY 1 cut(s) 214
RsaI GTAC 3 cut(s) 696, 950, 985
RsaNI GTAC 3 cut(s) 695, 949, 984
RseI CAYNNNNRTG 2 cut(s) 602, 875
SapI GCTCTTC 1 cut(s) 1485
SaqAI TTAA 2 cut(s) 1371, 1388
SatI GCNGC 5 cut(s) 159, 200, 341, 764, 881
Sau3AI GATC 2 cut(s) 214, 865
Sau96I GGNCC 1 cut(s) 1136
SchI GAGTC 3 cut(s) 399, 893, 1228
ScrFI CCNGG 3 cut(s) 441, 1035, 1355
SduI GDGCHC 1 cut(s) 469
SfaNI GCATC 6 cut(s) 169, 230, 306, 493, 597, 1242
SfcI CTRYAG 4 cut(s) 37, 183, 768, 1202
SfuI TTCGAA 1 cut(s) 294
SmiMI CAYNNNNRTG 2 cut(s) 602, 875
SphI GCATGC 1 cut(s) 880
SsiI CCGC 3 cut(s) 200, 916, 1013
SspMI CTAG 3 cut(s) 87, 434, 750
StyD4I CCNGG 3 cut(s) 439, 1033, 1353
StyI CCWWGG 2 cut(s) 612, 1139
TaaI ACNGT 3 cut(s) 694, 953, 983
TaiI ACGT 3 cut(s) 142, 313, 1332
TaqI TCGA 2 cut(s) 294, 978
TatI WGTACW 3 cut(s) 694, 948, 983
TauI GCSGC 1 cut(s) 202
TfiI GAWTC 5 cut(s) 549, 1066, 1107, 1267, 1376
Tru1I TTAA 2 cut(s) 1371, 1388
Tru9I TTAA 2 cut(s) 1371, 1388
TscAI CASTG 4 cut(s) 697, 808, 877, 1056
TseFI GTSAC 1 cut(s) 839
TseI GCWGC 4 cut(s) 158, 340, 763, 880
Tsp45I GTSAC 1 cut(s) 839
TspDTI ATGAA 5 cut(s) 15, 221, 339, 746, 1206
TspGWI ACGGA 1 cut(s) 751
TspRI CASTG 4 cut(s) 697, 808, 877, 1056
Van91I CCANNNNNTGG 1 cut(s) 653
VneI GTGCAC 1 cut(s) 465
XapI RAATTY 3 cut(s) 489, 588, 790
XceI RCATGY 4 cut(s) 110, 646, 880, 1319
XmiI GTMKAC 1 cut(s) 1180
XmnI GAANNNNTTC 1 cut(s) 1106
XspI CTAG 3 cut(s) 87, 434, 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.