Rroxscaffold_4G00319900

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001519
Physical Location & Seq
Forward (+)
48092575 .. 48095117
2543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00319900.1

Sequence Viewer

Length: 1137 bp
ATGGCACTCACTTCCCAGACAAGTTTATCATACTACTGCAGAAGCATTGGCTTCTCTTCCTTAGCATTTTATTCTACTACTGCGAGGAAGATACAGATTTTGCATGCAGTCAATGTTGCAGGACAAAAACAAGGACGTAAAAGTGCGATAGTTGCTTCAAGTCCTATCACAGAAGATGCTGTAGTAGCTACAATGCCGCTGACGAAAGAAGATCTTGTAGGCCATCTTGCCTCTGGATGCAAGCCTAAAGAAAAATGGAGAATTGGCATGGAACATGAGAGGTTTGGCTTCAATTTAAAAAGTTTACGTCCCCTGCAATATCAACAAATTGCTGAGCTGCTAAATGGGATTGCTGAGAGATTTGAGTGGGAGAAAATCATGGAAGGTGATAACATTATTGGACTCAAACAGGGCAAGCAGAGTATATCACTTGAGCCTGGAGGTCAGTTTGAGCTTAGTGGTGCACCTCTTGAGACTCTGCATCAAGTTTGTATGGAAGTCGATTCACAACTCTATCAGCCGAAATGGAGCATTCAAGGCATACCTATGATGCCCAGGCGAAGATATGAGATTGCTAATAATTACATGCCAAAAGTTGTCTCGCTTGGACATGATTTGATGTCCAGGATTTGCACAGTACAGGTTAATCTGGACTTCAGTTATGAAGCAGACATGAAACGTAAAATTTGTGCTAGTCTTGCTTTGCAGCCTATAGCAACAGCCCTGTTAGCAAATTCTCCTTTTACTGAAGGAAAGCCAAATGGTTTTTTGAGCAGGAGAAGTCACATCTATACTGATACTGACAAGAATCGCACTGGCATGCTTCCTTTTGCCTTTGATAACTCCTTTGGGTTTGAGCGGTATGTTGATTATGCTCTTGATGTTCCCATCATCCCATGTACTATGTTTATCGGAAAAAGAAGTGTATTGAATGACTTTATGGCTGGAAAGCTTCATTGCCTTCCGGGCGAATTACCAACCCTGAATGATTGGGAAACTCATTTGGGAACTCTATATCCAGAGGTCAGGCTGAAGAGATACTTAGAGATGAGGGGTGCTGATGGAGGGCCTTGGAGGAGCTTATGTGCTTTGCCTGCATTTTGGATGATTGTTCCAGGTTATGTCATGGAAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

378

Amino Acids

42.48

Weight (kDa)

8.32

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCS2 PF04107 87 - 170 1.1e-06 Glutamate-cysteine ligase family 2(GCS2)
GCS2 PF04107 173 - 369 2.9e-51 Glutamate-cysteine ligase family 2(GCS2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 859
AciI CCGC 2 cut(s) 197, 859
AcsI RAATTY 2 cut(s) 684, 733
AcuI CTGAAG 3 cut(s) 640, 768, 1052
AfaI GTAC 2 cut(s) 639, 901
AgsI TTSAA 4 cut(s) 159, 292, 536, 931
AjnI CCWGG 4 cut(s) 436, 554, 623, 1114
AloI GAACNNNNNNTCC 2 cut(s) 1000, 1032
AluBI AGCT 5 cut(s) 188, 337, 454, 952, 1080
AluI AGCT 5 cut(s) 188, 337, 454, 952, 1080
Alw21I GWGCWC 1 cut(s) 466
Alw26I GTCTC 2 cut(s) 467, 604
Alw44I GTGCAC 1 cut(s) 462
AoxI GGCC 2 cut(s) 220, 1067
ApaLI GTGCAC 1 cut(s) 462
ApeKI GCWGC 2 cut(s) 337, 706
ApoI RAATTY 2 cut(s) 684, 733
AspS9I GGNCC 1 cut(s) 1067
AsuC2I CCSGG 1 cut(s) 966
AsuHPI GGTGA 1 cut(s) 398
BaeGI GKGCMC 1 cut(s) 466
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 2 cut(s) 324, 718
BccI CCATC 3 cut(s) 231, 896, 1055
BciT130I CCWGG 4 cut(s) 438, 556, 625, 1116
BcnI CCSGG 1 cut(s) 966
BcoDI GTCTC 2 cut(s) 467, 604
BfaI CTAG 1 cut(s) 693
BfmI CTRYAG 3 cut(s) 37, 180, 711
BglI GCCNNNNNGGC 1 cut(s) 966
BglII AGATCT 1 cut(s) 211
BisI GCNGC 3 cut(s) 197, 338, 707
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 3 cut(s) 198, 339, 708
Bme1390I CCNGG 5 cut(s) 438, 556, 625, 966, 1116
BmgT120I GGNCC 1 cut(s) 1067
BmrFI CCNGG 5 cut(s) 438, 556, 625, 966, 1116
BmsI GCATC 4 cut(s) 166, 227, 490, 540
BpmI CTGGAG 1 cut(s) 459
Bpu10I CCTNAGC 1 cut(s) 61
Bpu1102I GCTNAGC 1 cut(s) 333
BpuEI CTTGAG 2 cut(s) 452, 491
BpuMI CCSGG 1 cut(s) 966
BsaJI CCNNGG 2 cut(s) 554, 1070
BsaXI ACNNNNNCTCC 2 cut(s) 1069, 1099
Bse1I ACTGG 1 cut(s) 820
Bse3DI GCAATG 1 cut(s) 955
BseBI CCWGG 4 cut(s) 438, 556, 625, 1116
BseDI CCNNGG 2 cut(s) 554, 1070
BseGI GGATG 3 cut(s) 242, 891, 1110
BseMI GCAATG 1 cut(s) 955
BseMII CTCAG 2 cut(s) 324, 345
BseNI ACTGG 1 cut(s) 820
BseRI GAGGAG 1 cut(s) 1090
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 2 cut(s) 324, 718
BshFI GGCC 2 cut(s) 222, 1069
BsiHKAI GWGCWC 1 cut(s) 466
BsiSI CCGG 1 cut(s) 965
BslFI GGGAC 1 cut(s) 294
BsmAI GTCTC 2 cut(s) 467, 604
BsmFI GGGAC 1 cut(s) 294
BsmI GAATGC 1 cut(s) 531
BsnI GGCC 2 cut(s) 222, 1069
Bsp1286I GDGCHC 1 cut(s) 466
Bsp143I GATC 1 cut(s) 211
Bsp1720I GCTNAGC 1 cut(s) 333
BspACI CCGC 2 cut(s) 197, 859
BspANI GGCC 2 cut(s) 222, 1069
BspCNI CTCAG 2 cut(s) 325, 346
BspMAI CTGCAG 1 cut(s) 41
BsrBI CCGCTC 1 cut(s) 859
BsrDI GCAATG 1 cut(s) 955
BsrI ACTGG 1 cut(s) 820
BssECI CCNNGG 2 cut(s) 554, 1070
BssMI GATC 1 cut(s) 211
BssT1I CCWWGG 1 cut(s) 1070
Bst2UI CCWGG 4 cut(s) 438, 556, 625, 1116
Bst4CI ACNGT 1 cut(s) 637
Bst6I CTCTTC 2 cut(s) 61, 1028
BstC8I GCNNGC 5 cut(s) 105, 242, 416, 821, 1095
BstDEI CTNAG 5 cut(s) 61, 333, 354, 455, 1042
BstF5I GGATG 3 cut(s) 242, 891, 1110
BstKTI GATC 1 cut(s) 214
BstMAI GTCTC 2 cut(s) 467, 604
BstMBI GATC 1 cut(s) 211
BstMWI GCNNNNNNNGC 7 cut(s) 152, 185, 537, 698, 728, 966, 1094
BstNI CCWGG 4 cut(s) 438, 556, 625, 1116
BstNSI RCATGY 3 cut(s) 107, 589, 823
BstSCI CCNGG 5 cut(s) 436, 554, 623, 964, 1114
BstSFI CTRYAG 3 cut(s) 37, 180, 711
BstSLI GKGCMC 1 cut(s) 466
BstV1I GCAGC 2 cut(s) 324, 718
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BsuRI GGCC 2 cut(s) 222, 1069
BtsCI GGATG 3 cut(s) 242, 891, 1110
BtsIMutI CAGTG 1 cut(s) 813
Cac8I GCNNGC 5 cut(s) 105, 242, 416, 821, 1095
Cfr13I GGNCC 1 cut(s) 1067
Csp6I GTAC 2 cut(s) 638, 900
CviQI GTAC 2 cut(s) 638, 900
DdeI CTNAG 5 cut(s) 61, 333, 354, 455, 1042
DpnI GATC 1 cut(s) 213
DpnII GATC 1 cut(s) 211
DraI TTTAAA 1 cut(s) 297
Eam1104I CTCTTC 2 cut(s) 61, 1028
EarI CTCTTC 2 cut(s) 61, 1028
Eco130I CCWWGG 1 cut(s) 1070
Eco57I CTGAAG 3 cut(s) 640, 768, 1052
EcoO109I RGGNCCY 1 cut(s) 1067
EcoRII CCWGG 4 cut(s) 436, 554, 623, 1114
EcoT14I CCWWGG 1 cut(s) 1070
ErhI CCWWGG 1 cut(s) 1070
FalI AAGNNNNNCTT 4 cut(s) 198, 230, 1025, 1057
FaqI GGGAC 1 cut(s) 294
Fnu4HI GCNGC 3 cut(s) 197, 338, 707
FokI GGATG 3 cut(s) 249, 878, 1117
Fsp4HI GCNGC 3 cut(s) 197, 338, 707
FspBI CTAG 1 cut(s) 693
GluI GCNGC 3 cut(s) 197, 338, 707
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 2 cut(s) 222, 1069
HapII CCGG 1 cut(s) 965
HindIII AAGCTT 1 cut(s) 950
HinfI GANTC 4 cut(s) 402, 475, 503, 808
HpaII CCGG 1 cut(s) 965
HphI GGTGA 1 cut(s) 398
Hpy166II GTNNAC 2 cut(s) 305, 464
Hpy188I TCNGA 1 cut(s) 914
Hpy188III TCNNGA 5 cut(s) 234, 470, 650, 878, 1019
Hpy8I GTNNAC 2 cut(s) 305, 464
HpyAV CCTTC 4 cut(s) 377, 743, 971, 1124
HpyCH4III ACNGT 1 cut(s) 637
HpyCH4IV ACGT 3 cut(s) 136, 307, 679
HpyF10VI GCNNNNNNNGC 7 cut(s) 152, 185, 537, 698, 728, 966, 1094
HpyF3I CTNAG 5 cut(s) 61, 333, 354, 455, 1042
HpySE526I ACGT 3 cut(s) 136, 307, 679
Kzo9I GATC 1 cut(s) 211
LmnI GCTCC 2 cut(s) 528, 1077
Lsp1109I GCAGC 2 cut(s) 324, 718
LweI GCATC 4 cut(s) 166, 227, 490, 540
MaeI CTAG 1 cut(s) 693
MaeII ACGT 3 cut(s) 136, 307, 679
MaeIII GTNAC 1 cut(s) 782
MalI GATC 1 cut(s) 213
MbiI CCGCTC 1 cut(s) 859
MboI GATC 1 cut(s) 211
MboII GAAGA 6 cut(s) 48, 100, 185, 221, 573, 1045
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 1 cut(s) 466
MluCI AATT 7 cut(s) 261, 292, 327, 580, 684, 733, 971
MlyI GAGTC 2 cut(s) 396, 469
MnlI CCTC 9 cut(s) 78, 241, 273, 434, 477, 1015, 1044, 1058, 1068
MseI TTAA 3 cut(s) 296, 645, 1135
MslI CAYNNNNRTG 2 cut(s) 545, 818
MspA1I CMGCKG 1 cut(s) 199
MspI CCGG 1 cut(s) 965
MspR9I CCNGG 5 cut(s) 438, 556, 625, 966, 1116
Mva1269I GAATGC 1 cut(s) 531
MvaI CCWGG 4 cut(s) 438, 556, 625, 1116
MwoI GCNNNNNNNGC 7 cut(s) 152, 185, 537, 698, 728, 966, 1094
NciI CCSGG 1 cut(s) 966
NdeII GATC 1 cut(s) 211
NmuCI GTSAC 1 cut(s) 782
NspI RCATGY 3 cut(s) 107, 589, 823
PaeI GCATGC 2 cut(s) 107, 823
PctI GAATGC 1 cut(s) 531
PfeI GAWTC 2 cut(s) 503, 808
PfoI TCCNGGA 1 cut(s) 623
PkrI GCNGC 3 cut(s) 198, 339, 708
PleI GAGTC 2 cut(s) 396, 469
PpsI GAGTC 2 cut(s) 396, 469
Psp6I CCWGG 4 cut(s) 436, 554, 623, 1114
PspGI CCWGG 4 cut(s) 436, 554, 623, 1114
PspPI GGNCC 1 cut(s) 1067
PstI CTGCAG 1 cut(s) 41
PsuI RGATCY 1 cut(s) 211
RsaI GTAC 2 cut(s) 639, 901
RsaNI GTAC 2 cut(s) 638, 900
RseI CAYNNNNRTG 2 cut(s) 545, 818
SaqAI TTAA 3 cut(s) 296, 645, 1135
SatI GCNGC 3 cut(s) 197, 338, 707
Sau3AI GATC 1 cut(s) 211
Sau96I GGNCC 1 cut(s) 1067
SchI GAGTC 2 cut(s) 396, 469
ScrFI CCNGG 5 cut(s) 438, 556, 625, 966, 1116
SduI GDGCHC 1 cut(s) 466
SfaNI GCATC 4 cut(s) 166, 227, 490, 540
SfcI CTRYAG 3 cut(s) 37, 180, 711
SmiMI CAYNNNNRTG 2 cut(s) 545, 818
SmlI CTYRAG 2 cut(s) 431, 470
SmoI CTYRAG 2 cut(s) 431, 470
SphI GCATGC 2 cut(s) 107, 823
Sse9I AATT 7 cut(s) 261, 292, 327, 580, 684, 733, 971
SsiI CCGC 2 cut(s) 197, 859
SspMI CTAG 1 cut(s) 693
StyD4I CCNGG 5 cut(s) 436, 554, 623, 964, 1114
StyI CCWWGG 1 cut(s) 1070
TaaI ACNGT 1 cut(s) 637
TaiI ACGT 3 cut(s) 139, 310, 682
TaqI TCGA 1 cut(s) 501
TasI AATT 7 cut(s) 261, 292, 327, 580, 684, 733, 971
TatI WGTACW 2 cut(s) 637, 899
TauI GCSGC 1 cut(s) 199
TfiI GAWTC 2 cut(s) 503, 808
Tru1I TTAA 3 cut(s) 296, 645, 1135
Tru9I TTAA 3 cut(s) 296, 645, 1135
TscAI CASTG 1 cut(s) 820
TseFI GTSAC 1 cut(s) 782
TseI GCWGC 2 cut(s) 337, 706
Tsp45I GTSAC 1 cut(s) 782
TspDTI ATGAA 3 cut(s) 678, 689, 944
TspRI CASTG 1 cut(s) 820
VneI GTGCAC 1 cut(s) 462
XapI RAATTY 2 cut(s) 684, 733
XceI RCATGY 3 cut(s) 107, 589, 823
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XspI CTAG 1 cut(s) 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.