Rmu_sc0002263.1_g000037

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002263.1
OG0001519
Physical Location & Seq
Forward (+)
162181 .. 165195
3015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002263.1_g000037.1.cds

Sequence Viewer

Length: 519 bp
atggtgtatacaagggggtcggaggagagaacgagagtggtggtggctgccttccgggtgaataaggcaggaaagcttcactgccttccgggtgaattaccgaccctgaatgattgggagaatcgtttgggaactctatatccagaggtcaggctgaagagatacttagagatgaggggggctgatggagggccttggaggcggttatgtgctttgcctgcattttgggtgggtttactgcatgatgaggtttccctgcagaatgtgttagacatgatagctgattggactcctaaagaaagacagatgctgagagataaggttccgaaaacgggtctaaagacaccatttcgtgatggattactcaagcaagttgccgaagatgtagtaaagtttgcaaaggatggcctggaaagaagaggctttaaggaatcagggttcctaaatgaagcggaggagaatgcagctctggcagagaatgttgctcctgcggactactttcaagagcctgcaaaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

172

Amino Acids

19.56

Weight (kDa)

7.75

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 8
AciI CCGC 3 cut(s) 202, 452, 491
AcuI CTGAAG 1 cut(s) 176
AfiI CCNNNNNNNGG 1 cut(s) 332
AgsI TTSAA 1 cut(s) 503
AjnI CCWGG 1 cut(s) 408
AloI GAACNNNNNNTCC 4 cut(s) 124, 156, 422, 454
AluBI AGCT 3 cut(s) 76, 281, 467
AluI AGCT 3 cut(s) 76, 281, 467
AlwNI CAGNNNCTG 1 cut(s) 310
AoxI GGCC 2 cut(s) 191, 406
ApeKI GCWGC 2 cut(s) 47, 464
AspS9I GGNCC 1 cut(s) 191
AsuC2I CCSGG 2 cut(s) 56, 90
AsuHPI GGTGA 2 cut(s) 70, 104
BbvI GCAGC 2 cut(s) 34, 476
BccI CCATC 3 cut(s) 179, 350, 398
BciT130I CCWGG 1 cut(s) 410
BcnI CCSGG 2 cut(s) 56, 90
BfmI CTRYAG 1 cut(s) 257
BglI GCCNNNNNGGC 1 cut(s) 199
BisI GCNGC 2 cut(s) 48, 465
BlsI GCNGC 2 cut(s) 49, 466
Bme1390I CCNGG 3 cut(s) 56, 90, 410
BmgT120I GGNCC 1 cut(s) 191
BmiI GGNNCC 2 cut(s) 324, 440
BmrFI CCNGG 3 cut(s) 56, 90, 410
BmsI GCATC 1 cut(s) 297
BpuEI CTTGAG 1 cut(s) 350
BpuMI CCSGG 2 cut(s) 56, 90
BsaJI CCNNGG 1 cut(s) 194
Bsc4I CCNNNNNNNGG 1 cut(s) 332
BseBI CCWGG 1 cut(s) 410
BseDI CCNNGG 1 cut(s) 194
BseGI GGATG 1 cut(s) 409
BseLI CCNNNNNNNGG 1 cut(s) 332
BseMII CTCAG 1 cut(s) 302
BseRI GAGGAG 2 cut(s) 38, 470
BseXI GCAGC 2 cut(s) 34, 476
BshFI GGCC 2 cut(s) 193, 408
BsiSI CCGG 2 cut(s) 55, 89
BslI CCNNNNNNNGG 1 cut(s) 332
BsmI GAATGC 1 cut(s) 466
BsnI GGCC 2 cut(s) 193, 408
BspACI CCGC 3 cut(s) 202, 452, 491
BspANI GGCC 2 cut(s) 193, 408
BspCNI CTCAG 1 cut(s) 303
BspLI GGNNCC 2 cut(s) 324, 440
BspMAI CTGCAG 1 cut(s) 261
BssECI CCNNGG 1 cut(s) 194
BssNAI GTATAC 1 cut(s) 9
BssT1I CCWWGG 1 cut(s) 194
Bst1107I GTATAC 1 cut(s) 9
Bst2UI CCWGG 1 cut(s) 410
Bst6I CTCTTC 2 cut(s) 152, 412
BstC8I GCNNGC 2 cut(s) 219, 510
BstDEI CTNAG 2 cut(s) 166, 311
BstF5I GGATG 1 cut(s) 409
BstMWI GCNNNNNNNGC 3 cut(s) 199, 218, 470
BstNI CCWGG 1 cut(s) 410
BstSCI CCNGG 3 cut(s) 54, 88, 408
BstSFI CTRYAG 1 cut(s) 257
BstV1I GCAGC 2 cut(s) 34, 476
BstZ17I GTATAC 1 cut(s) 9
BsuRI GGCC 2 cut(s) 193, 408
BtsCI GGATG 1 cut(s) 409
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 1 cut(s) 79
Cac8I GCNNGC 2 cut(s) 219, 510
CaiI CAGNNNCTG 1 cut(s) 310
Cfr13I GGNCC 1 cut(s) 191
CviAII CATG 2 cut(s) 242, 274
DdeI CTNAG 2 cut(s) 166, 311
Eam1104I CTCTTC 2 cut(s) 152, 412
EarI CTCTTC 2 cut(s) 152, 412
Eco130I CCWWGG 1 cut(s) 194
Eco57I CTGAAG 1 cut(s) 176
EcoO109I RGGNCCY 1 cut(s) 191
EcoRII CCWGG 1 cut(s) 408
EcoT14I CCWWGG 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 194
FaeI CATG 2 cut(s) 245, 277
FaiI YATR 5 cut(s) 9, 139, 208, 243, 275
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FatI CATG 2 cut(s) 241, 273
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 2 cut(s) 48, 465
FokI GGATG 1 cut(s) 416
Fsp4HI GCNGC 2 cut(s) 48, 465
GluI GCNGC 2 cut(s) 48, 465
HaeIII GGCC 2 cut(s) 193, 408
HapII CCGG 2 cut(s) 55, 89
Hin1II CATG 2 cut(s) 245, 277
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 3 cut(s) 121, 289, 431
HpaII CCGG 2 cut(s) 55, 89
HphI GGTGA 2 cut(s) 70, 104
Hpy166II GTNNAC 2 cut(s) 9, 236
Hpy188I TCNGA 2 cut(s) 22, 327
Hpy188III TCNNGA 3 cut(s) 143, 353, 503
Hpy8I GTNNAC 2 cut(s) 9, 236
HpyAV CCTTC 2 cut(s) 61, 95
HpyCH4V TGCA 6 cut(s) 221, 241, 259, 398, 464, 512
HpyF10VI GCNNNNNNNGC 3 cut(s) 199, 218, 470
HpyF3I CTNAG 2 cut(s) 166, 311
Hsp92II CATG 2 cut(s) 245, 277
LmnI GCTCC 1 cut(s) 490
Lsp1109I GCAGC 2 cut(s) 34, 476
LweI GCATC 1 cut(s) 297
MboII GAAGA 3 cut(s) 169, 392, 429
MluCI AATT 1 cut(s) 95
MlyI GAGTC 1 cut(s) 283
MnlI CCTC 8 cut(s) 16, 139, 168, 182, 192, 241, 413, 448
MseI TTAA 1 cut(s) 426
MspI CCGG 2 cut(s) 55, 89
MspR9I CCNGG 3 cut(s) 56, 90, 410
Mva1269I GAATGC 1 cut(s) 466
MvaI CCWGG 1 cut(s) 410
MwoI GCNNNNNNNGC 3 cut(s) 199, 218, 470
NciI CCSGG 2 cut(s) 56, 90
NlaIII CATG 2 cut(s) 245, 277
NlaIV GGNNCC 2 cut(s) 324, 440
PctI GAATGC 1 cut(s) 466
PfeI GAWTC 2 cut(s) 121, 431
PkrI GCNGC 2 cut(s) 49, 466
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
Psp6I CCWGG 1 cut(s) 408
PspGI CCWGG 1 cut(s) 408
PspN4I GGNNCC 2 cut(s) 324, 440
PspPI GGNCC 1 cut(s) 191
PstI CTGCAG 1 cut(s) 261
PstNI CAGNNNCTG 1 cut(s) 310
SaqAI TTAA 1 cut(s) 426
SatI GCNGC 2 cut(s) 48, 465
Sau96I GGNCC 1 cut(s) 191
SchI GAGTC 1 cut(s) 283
ScrFI CCNGG 3 cut(s) 56, 90, 410
SetI ASST 6 cut(s) 78, 150, 252, 283, 324, 469
SfaNI GCATC 1 cut(s) 297
SfcI CTRYAG 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
Sse9I AATT 1 cut(s) 95
SsiI CCGC 3 cut(s) 202, 452, 491
StyD4I CCNGG 3 cut(s) 54, 88, 408
StyI CCWWGG 1 cut(s) 194
TasI AATT 1 cut(s) 95
TfiI GAWTC 2 cut(s) 121, 431
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TscAI CASTG 1 cut(s) 86
TseI GCWGC 2 cut(s) 47, 464
TspDTI ATGAA 1 cut(s) 462
TspRI CASTG 1 cut(s) 86
XmiI GTMKAC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.