AT5G28780

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001547
Physical Location & Seq
Reverse (-)
10812907 .. 10814173
1267 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G28780.1

Sequence Viewer

Length: 1014 bp
ATGAGAGTGCGTGATAAAAACGGAGACGTTGGAACTTTTGTTGTCCTTGATGGTGAAGGCACCAAAATTGCTGGAAGAAGCGTTCGTGATGTTTTAAATGAATACATGGAGGCTGTAGGAAAAGATTCATATGAAGGAATAAACACCAAATGGAACACGAGCAGACACTGTACTTGCTGCTATAATGTAGGAAACGGAAACGCTCCAACATGTGAAACAGATGATGGTGAAGTCGTCGAAATGGACACTTCTTTCTTTTTGAAGCATAACGGAAATCGTCTCCAACAAGTCACAAAGGGAGCATATGTGCAATTCAGTGTCTCCCAACCCAACTTCCAATACTTGACCGAACGAGGAATTCTTACACCACACAATGAATATGTTGATGAGATAAATGCATACATGTTATCTCAGGTAGGAGGGGATTCAAAAGAATATTTAAGCTCATATAGCATTGGAAAAGCGGATACGATCGGAGCAGATTATGAAGCCCTCTACCATGTCAAGTATTTAAACTCTTTAGAGTTTCCTAGTTTGCCGAAACACAAAATAAGCCTAAAAAAAGGAGTTCCAATTATGCAGATGCGAAACTTTAACCAAAAGGAAGGTTTGTGTAACGGTACAAGACTGATTGTCACTAATTTGGGTGAACAAGTGATAGAAGCCCAAATAGTAACAGGCACGCATGCTGGTAAAATGGTTTCTATACCAAGATTTATCTTATCCCCACCACAATCAGAGCATCCTTTTACACTTCGAAGACAACAATTTCCAATGAGAGTTTGCTACGCCATGACCATCATAAAAAATCAAGGTCAAAGCTTAAAATCTGATGTTCTGTATCTACCAAATCCAGTTTTCAGTCATGTACAACTCTACGTAGCTCTTTCTAGAGTTACATCACCTATTGGCCTAACAATTCTACATGGAGATGACCAAAAAAATGATGAAGTTAAAAATATTGTCTACAAGGAATTCTACAATGACCTCCCAAAAGAAACTGACCCTTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N Herpes_Helicase PIF1

Protein Analysis

337

Amino Acids

37.87

Weight (kDa)

6.32

Isoelectric Point (pI)

30.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pif1_2B_dom PF21530 170 - 215 8.9e-19 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 59
AccI GTMKAC 1 cut(s) 966
AciI CCGC 1 cut(s) 464
AcsI RAATTY 2 cut(s) 357, 974
AfaI GTAC 3 cut(s) 172, 622, 870
AflIII ACRYGT 2 cut(s) 209, 402
AgsI TTSAA 2 cut(s) 262, 429
AhdI GACNNNNNGTC 1 cut(s) 632
AloI GAACNNNNNNTCC 2 cut(s) 66, 98
AluBI AGCT 3 cut(s) 444, 822, 884
AluI AGCT 3 cut(s) 444, 822, 884
Alw26I GTCTC 3 cut(s) 18, 284, 325
AlwNI CAGNNNCTG 1 cut(s) 168
AoxI GGCC 1 cut(s) 910
ApeKI GCWGC 1 cut(s) 177
ApoI RAATTY 2 cut(s) 357, 974
Asp700I GAANNNNTTC 1 cut(s) 124
AsuHPI GGTGA 4 cut(s) 65, 239, 659, 894
AsuII TTCGAA 1 cut(s) 757
BanI GGYRCC 1 cut(s) 59
BauI CACGAG 1 cut(s) 157
BbsI GAAGAC 1 cut(s) 766
BbvI GCAGC 1 cut(s) 164
BccI CCATC 3 cut(s) 44, 218, 806
BciVI GTATCC 1 cut(s) 460
BcoDI GTCTC 3 cut(s) 18, 284, 325
BfaI CTAG 2 cut(s) 531, 891
BfmI CTRYAG 1 cut(s) 114
BfuI GTATCC 1 cut(s) 460
BisI GCNGC 1 cut(s) 178
BlsI GCNGC 1 cut(s) 179
BmeRI GACNNNNNGTC 1 cut(s) 632
BmiI GGNNCC 1 cut(s) 61
BmsI GCATC 2 cut(s) 573, 751
BpiI GAAGAC 1 cut(s) 766
Bpu14I TTCGAA 1 cut(s) 757
BsaAI YACGTR 1 cut(s) 880
BsaXI ACNNNNNCTCC 2 cut(s) 291, 321
Bse1I ACTGG 1 cut(s) 854
BseGI GGATG 1 cut(s) 742
BseMII CTCAG 1 cut(s) 425
BseNI ACTGG 1 cut(s) 854
BseXI GCAGC 1 cut(s) 164
Bsh1285I CGRYCG 1 cut(s) 474
BshFI GGCC 1 cut(s) 912
BshNI GGYRCC 1 cut(s) 59
BsiEI CGRYCG 1 cut(s) 474
BsmAI GTCTC 3 cut(s) 18, 284, 325
BsmBI CGTCTC 2 cut(s) 18, 284
BsnI GGCC 1 cut(s) 912
Bsp119I TTCGAA 1 cut(s) 757
Bsp1407I TGTACA 1 cut(s) 868
Bsp143I GATC 1 cut(s) 471
BspACI CCGC 1 cut(s) 464
BspANI GGCC 1 cut(s) 912
BspCNI CTCAG 1 cut(s) 424
BspLI GGNNCC 1 cut(s) 61
BspT104I TTCGAA 1 cut(s) 757
BspT107I GGYRCC 1 cut(s) 59
BsrGI TGTACA 1 cut(s) 868
BsrI ACTGG 1 cut(s) 854
BssMI GATC 1 cut(s) 471
BssSI CACGAG 1 cut(s) 157
Bst2BI CACGAG 1 cut(s) 157
Bst4CI ACNGT 2 cut(s) 170, 620
BstAUI TGTACA 1 cut(s) 868
BstBAI YACGTR 1 cut(s) 880
BstBI TTCGAA 1 cut(s) 757
BstC8I GCNNGC 2 cut(s) 683, 687
BstDEI CTNAG 1 cut(s) 411
BstF5I GGATG 1 cut(s) 742
BstKTI GATC 1 cut(s) 474
BstMAI GTCTC 3 cut(s) 18, 284, 325
BstMBI GATC 1 cut(s) 471
BstMCI CGRYCG 1 cut(s) 474
BstMWI GCNNNNNNNGC 1 cut(s) 450
BstNSI RCATGY 3 cut(s) 213, 406, 689
BstSFI CTRYAG 1 cut(s) 114
BstSNI TACGTA 1 cut(s) 880
BstV1I GCAGC 1 cut(s) 164
BstV2I GAAGAC 1 cut(s) 766
BsuI GTATCC 1 cut(s) 460
BsuRI GGCC 1 cut(s) 912
BtsCI GGATG 1 cut(s) 742
BtsIMutI CAGTG 2 cut(s) 166, 322
Cac8I GCNNGC 2 cut(s) 683, 687
CaiI CAGNNNCTG 1 cut(s) 168
Csp6I GTAC 3 cut(s) 171, 621, 869
CviAII CATG 8 cut(s) 106, 210, 403, 500, 686, 793, 866, 926
CviJI RGCY 8 cut(s) 113, 444, 491, 555, 665, 822, 884, 912
CviKI_1 RGCY 8 cut(s) 113, 444, 491, 555, 665, 822, 884, 912
CviQI GTAC 3 cut(s) 171, 621, 869
DdeI CTNAG 1 cut(s) 411
DpnI GATC 1 cut(s) 473
DpnII GATC 1 cut(s) 471
DraI TTTAAA 2 cut(s) 96, 513
DriI GACNNNNNGTC 1 cut(s) 632
Eam1105I GACNNNNNGTC 1 cut(s) 632
Eco105I TACGTA 1 cut(s) 880
EcoRI GAATTC 2 cut(s) 357, 974
EcoT22I ATGCAT 1 cut(s) 400
Esp3I CGTCTC 2 cut(s) 18, 284
FaeI CATG 8 cut(s) 109, 213, 406, 503, 689, 796, 869, 929
FatI CATG 8 cut(s) 105, 209, 402, 499, 685, 792, 865, 925
FauNDI CATATG 2 cut(s) 130, 304
FblI GTMKAC 1 cut(s) 966
Fnu4HI GCNGC 1 cut(s) 178
FokI GGATG 1 cut(s) 729
Fsp4HI GCNGC 1 cut(s) 178
FspBI CTAG 2 cut(s) 531, 891
GluI GCNGC 1 cut(s) 178
HaeIII GGCC 1 cut(s) 912
Hin1II CATG 8 cut(s) 109, 213, 406, 503, 689, 796, 869, 929
HindIII AAGCTT 1 cut(s) 820
HinfI GANTC 2 cut(s) 125, 425
HphI GGTGA 4 cut(s) 65, 239, 659, 894
Hpy166II GTNNAC 2 cut(s) 650, 967
Hpy188I TCNGA 3 cut(s) 476, 739, 832
Hpy188III TCNNGA 2 cut(s) 86, 891
Hpy8I GTNNAC 2 cut(s) 650, 967
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 3 cut(s) 50, 128, 599
HpyCH4III ACNGT 2 cut(s) 170, 620
HpyCH4IV ACGT 2 cut(s) 27, 879
HpyCH4V TGCA 3 cut(s) 310, 398, 580
HpyF10VI GCNNNNNNNGC 1 cut(s) 450
HpyF3I CTNAG 1 cut(s) 411
HpySE526I ACGT 2 cut(s) 27, 879
Hsp92II CATG 8 cut(s) 109, 213, 406, 503, 689, 796, 869, 929
Kzo9I GATC 1 cut(s) 471
LmnI GCTCC 3 cut(s) 208, 299, 476
LpnPI CCDG 5 cut(s) 57, 398, 663, 675, 867
Lsp1109I GCAGC 1 cut(s) 164
LweI GCATC 2 cut(s) 573, 751
MaeI CTAG 2 cut(s) 531, 891
MaeII ACGT 2 cut(s) 27, 879
MaeIII GTNAC 5 cut(s) 289, 614, 634, 673, 895
MalI GATC 1 cut(s) 473
MboI GATC 1 cut(s) 471
MboII GAAGA 2 cut(s) 87, 771
MluCI AATT 8 cut(s) 66, 311, 357, 573, 640, 767, 918, 974
MmeI TCCRAC 3 cut(s) 10, 230, 307
MnlI CCTC 5 cut(s) 103, 347, 413, 503, 998
Mph1103I ATGCAT 1 cut(s) 400
MroXI GAANNNNTTC 1 cut(s) 124
MseI TTAA 6 cut(s) 95, 440, 512, 594, 824, 954
MslI CAYNNNNRTG 1 cut(s) 930
MwoI GCNNNNNNNGC 1 cut(s) 450
NdeI CATATG 2 cut(s) 130, 304
NdeII GATC 1 cut(s) 471
NlaIII CATG 8 cut(s) 109, 213, 406, 503, 689, 796, 869, 929
NlaIV GGNNCC 1 cut(s) 61
NmuCI GTSAC 2 cut(s) 289, 634
NsiI ATGCAT 1 cut(s) 400
NspI RCATGY 3 cut(s) 213, 406, 689
NspV TTCGAA 1 cut(s) 757
PaeI GCATGC 1 cut(s) 689
PciI ACATGT 2 cut(s) 209, 402
PdmI GAANNNNTTC 1 cut(s) 124
PfeI GAWTC 2 cut(s) 125, 425
PkrI GCNGC 1 cut(s) 179
Ple19I CGATCG 1 cut(s) 474
Ppu21I YACGTR 1 cut(s) 880
PscI ACATGT 2 cut(s) 209, 402
PspN4I GGNNCC 1 cut(s) 61
PstNI CAGNNNCTG 1 cut(s) 168
PvuI CGATCG 1 cut(s) 474
RsaI GTAC 3 cut(s) 172, 622, 870
RsaNI GTAC 3 cut(s) 171, 621, 869
RseI CAYNNNNRTG 1 cut(s) 930
SaqAI TTAA 6 cut(s) 95, 440, 512, 594, 824, 954
SatI GCNGC 1 cut(s) 178
Sau3AI GATC 1 cut(s) 471
SfaNI GCATC 2 cut(s) 573, 751
SfcI CTRYAG 1 cut(s) 114
SfuI TTCGAA 1 cut(s) 757
SmiMI CAYNNNNRTG 1 cut(s) 930
SnaBI TACGTA 1 cut(s) 880
SphI GCATGC 1 cut(s) 689
Sse9I AATT 8 cut(s) 66, 311, 357, 573, 640, 767, 918, 974
SsiI CCGC 1 cut(s) 464
SspI AATATT 2 cut(s) 437, 961
SspMI CTAG 2 cut(s) 531, 891
TaaI ACNGT 2 cut(s) 170, 620
TaiI ACGT 2 cut(s) 30, 882
TaqI TCGA 2 cut(s) 237, 757
TaqII GACCGA 1 cut(s) 362
TasI AATT 8 cut(s) 66, 311, 357, 573, 640, 767, 918, 974
TatI WGTACW 2 cut(s) 170, 868
TfiI GAWTC 2 cut(s) 125, 425
Tru1I TTAA 6 cut(s) 95, 440, 512, 594, 824, 954
Tru9I TTAA 6 cut(s) 95, 440, 512, 594, 824, 954
TscAI CASTG 2 cut(s) 173, 322
TseFI GTSAC 2 cut(s) 289, 634
TseI GCWGC 1 cut(s) 177
Tsp45I GTSAC 2 cut(s) 289, 634
TspDTI ATGAA 6 cut(s) 114, 117, 147, 390, 501, 963
TspGWI ACGGA 3 cut(s) 36, 210, 285
TspRI CASTG 2 cut(s) 173, 322
XapI RAATTY 2 cut(s) 357, 974
XbaI TCTAGA 1 cut(s) 890
XceI RCATGY 3 cut(s) 213, 406, 689
XmiI GTMKAC 1 cut(s) 966
XmnI GAANNNNTTC 1 cut(s) 124
XspI CTAG 2 cut(s) 531, 891
Zsp2I ATGCAT 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.