FvH4_3g10331

G-quadruplex DNA unwinding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001547
Physical Location & Seq
Reverse (-)
6037670 .. 6039936
2267 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g10331.t1

Sequence Viewer

Length: 1788 bp
ATGACTCGAAAGAAAAGAATTCGGGAGGAAAATTTGTCAGCTGCATCGTCTGAACAACTTGGGTGCAGTGGTCCCTCCATGAATGCTTCTGTTAACAGTAGACCGGGTGGTGCCAATGCATCAAATGATTGTGCTGAGTCTTCGTCTACAAGAGGAATCGATGCTCTGAGTATATCGTCTGCATATGTAGATGTCGGAGATATGACAAACGTTTGTGTTGATTGTCATGCATTGTTTTGGTTGCAAGAGTCAAAATTTATTCCAAGGAATGGAAGTCCGGTCTATACAGGCTGCTGCCAGGAAGGTCGGATTAGGCTCCCGCCTGTTAGACCTGCTCCAGTTTTTTTAGAATACTTGCTTGATTCCACTAATGGTCAAAAGAGTGTGCAGTTTAGAAACAATATTCGGAGTTATAATACCATGTTTGCATTTACTTCAATGGGAGCTAATGTAGATAAGACGGTGACTGATGGTCATGGTCCTTATGTTTTTAAAATTAGTGGACAGGTATACCATCTTATGGGTTCACTTATTCCTCCAGAAGGTAAGTGCCCTAAGTTTGCACAATTGTACATTTATGATACTGAGAATGAAGTTGCAAATCGACAAAAAGCTGTTGACGGTTTAGACTCGGAAATTGTTACTAGATTGATTGATATGCTTGATGAAAGCAATGAATTGGTTCAGTATTTTCGTTCTGTTCGAAATAAGTATAATGAAAGATCTCTGCGTTGTTTAAACTTGACACTTCTTGACAATGAAAGAATAAAAGATAGGGAATACGATGTGCCAAGTAGTAGTGAAGTTGCTGGCTTGATTGTTGGTGACATTGGGTTGTATGAAGCTGATAGGGATGTAGTTGTCGATACATTTGAAAATGGTTTGCAGCGGATATCAAGATTACATCCAAAATTCATGTCATTTCAATACCCATTACTCTTCCCTTATGGGGAGGATGGATATCATGTTGGTTTGAAATTATGCGCACGCAAAGATAATGAGAATCGCAAGCGAGAAAGGATGTCCATGTGTGATTTTATGTCATACCAAATACATGAGCGTGCAAATGAATCTTCCACTTTGCTGAAAGGAGGCCGACTTTTTCAACAGTTTTTGGTTGATGCCTATGCAAATGTTGAAGAAAACCGTTTGTTTTATATTAGAAAAAATCAACCCGATATTAGGAGCGAGACTTATGTGGGCATATGTGATGCTTCAATAAGAGAACATGATGGTGAAAATGTAGGCAAACGAATTATTTTACCAAGCACTCATACAGGAAGTCCGCGGTATATGAACAAACATTACCATGATGCTATGGCTTTGTGTCGGAAGTATGGCCCCCCTGATTTGTTCATAACACTTACTTGCAATCCAAAATGGCCTGAAATTGAAAGAGCATTGTTGAACAAACATGGTAGCAAACCTGAGGATCGCCCAGATATTGTTGCAAGAATTTTTAAGATGAAACATGATGATCTTATTAGTTACGTTAAATCTGGCAAGCCATTTGGGAAGGCTATTGCAGATGTTGCGACAATTGAGTTCCAAAAACGGGGATTACCTCACTCTCACATGTTATTTTGGTTGGCTGAGAATAACAAGTGTTATTCGGCTGTTGACATTGATTCAATAATCTTTGCTGAACTACCTGATAAGAAGGTCAATCCAACTTTATATGATATTGTGAATCAATTTATGATCCATGGTCCATGTGGTGTGCTGAATCCTAAAGCTCCATTCAACCTTGATAAAATATCTGTTCAAGTATATAAGCAAAGGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

596

Amino Acids

67.42

Weight (kDa)

7.52

Isoelectric Point (pI)

39.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 346 - 527 3.7e-59 Helitron helicase-like domain at N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
Acc16I TGCGCA 1 cut(s) 985
Acc36I ACCTGC 1 cut(s) 340
AccB1I GGYRCC 1 cut(s) 110
AccB7I CCANNNNNTGG 2 cut(s) 269, 520
AccI GTMKAC 3 cut(s) 100, 146, 510
AccII CGCG 1 cut(s) 1288
AciI CCGC 4 cut(s) 320, 889, 1286, 1288
AclI AACGTT 1 cut(s) 210
AclWI GGATC 2 cut(s) 1440, 1696
AcsI RAATTY 5 cut(s) 18, 31, 254, 911, 1455
AfaI GTAC 1 cut(s) 572
AfiI CCNNNNNNNGG 6 cut(s) 269, 520, 542, 949, 1182, 1555
AflIII ACRYGT 1 cut(s) 1575
AhdI GACNNNNNGTC 1 cut(s) 471
AjnI CCWGG 1 cut(s) 297
AluBI AGCT 5 cut(s) 41, 446, 614, 845, 1736
AluI AGCT 5 cut(s) 41, 446, 614, 845, 1736
Alw26I GTCTC 1 cut(s) 1184
AlwI GGATC 2 cut(s) 1440, 1696
AoxI GGCC 3 cut(s) 1093, 1339, 1382
ApeKI GCWGC 4 cut(s) 41, 291, 294, 886
ApoI RAATTY 5 cut(s) 18, 31, 254, 911, 1455
ArsI GACNNNNNNTTYG 2 cut(s) 902, 934
Asp700I GAANNNNTTC 1 cut(s) 681
AspLEI GCGC 1 cut(s) 986
AspS9I GGNCC 5 cut(s) 71, 479, 1340, 1709, 1782
AsuC2I CCSGG 1 cut(s) 105
AsuHPI GGTGA 3 cut(s) 475, 836, 1247
AsuII TTCGAA 1 cut(s) 703
AvaII GGWCC 4 cut(s) 71, 479, 1709, 1782
AxyI CCTNAGG 1 cut(s) 1428
BaeGI GKGCMC 1 cut(s) 554
BanI GGYRCC 1 cut(s) 110
BbsI GAAGAC 1 cut(s) 132
BbvI GCAGC 4 cut(s) 28, 278, 281, 898
BccI CCATC 4 cut(s) 464, 522, 950, 1226
BciT130I CCWGG 1 cut(s) 299
BcnI CCSGG 1 cut(s) 105
BcoDI GTCTC 1 cut(s) 1184
BfaI CTAG 1 cut(s) 645
BfuAI ACCTGC 1 cut(s) 340
BglII AGATCT 1 cut(s) 722
BisI GCNGC 4 cut(s) 42, 292, 295, 887
BlsI GCNGC 4 cut(s) 43, 293, 296, 888
Bme1390I CCNGG 2 cut(s) 105, 299
Bme18I GGWCC 4 cut(s) 71, 479, 1709, 1782
BmeRI GACNNNNNGTC 1 cut(s) 471
BmgT120I GGNCC 5 cut(s) 71, 479, 1340, 1709, 1782
BmiI GGNNCC 4 cut(s) 73, 112, 317, 1342
BmrFI CCNGG 2 cut(s) 105, 299
BmsI GCATC 6 cut(s) 53, 128, 151, 1111, 1201, 1303
BpiI GAAGAC 1 cut(s) 132
BpmI CTGGAG 2 cut(s) 321, 522
Bpu14I TTCGAA 1 cut(s) 703
BpuMI CCSGG 1 cut(s) 105
Bsa29I ATCGAT 1 cut(s) 159
BsaBI GATNNNNATC 1 cut(s) 960
BsaJI CCNNGG 3 cut(s) 263, 1286, 1705
BsaWI WCCGGW 1 cut(s) 277
BsaXI ACNNNNNCTCC 2 cut(s) 435, 465
Bsc4I CCNNNNNNNGG 6 cut(s) 269, 520, 542, 949, 1182, 1555
Bse1I ACTGG 1 cut(s) 338
Bse21I CCTNAGG 1 cut(s) 1428
Bse3DI GCAATG 1 cut(s) 679
Bse8I GATNNNNATC 1 cut(s) 960
BseBI CCWGG 1 cut(s) 299
BseCI ATCGAT 1 cut(s) 159
BseDI CCNNGG 3 cut(s) 263, 1286, 1705
BseGI GGATG 4 cut(s) 859, 904, 961, 1026
BseJI GATNNNNATC 1 cut(s) 960
BseLI CCNNNNNNNGG 6 cut(s) 269, 520, 542, 949, 1182, 1555
BseMI GCAATG 1 cut(s) 679
BseMII CTCAG 5 cut(s) 126, 158, 576, 1419, 1584
BseNI ACTGG 1 cut(s) 338
BseSI GKGCMC 1 cut(s) 554
BseXI GCAGC 4 cut(s) 28, 278, 281, 898
BsgI GTGCAG 2 cut(s) 85, 407
Bsh1236I CGCG 1 cut(s) 1288
BshFI GGCC 3 cut(s) 1095, 1341, 1384
BshNI GGYRCC 1 cut(s) 110
BshVI ATCGAT 1 cut(s) 159
BsiSI CCGG 2 cut(s) 104, 278
BslFI GGGAC 1 cut(s) 57
BslI CCNNNNNNNGG 6 cut(s) 269, 520, 542, 949, 1182, 1555
BsmAI GTCTC 1 cut(s) 1184
BsmFI GGGAC 1 cut(s) 57
BsmI GAATGC 1 cut(s) 88
BsnI GGCC 3 cut(s) 1095, 1341, 1384
Bsp119I TTCGAA 1 cut(s) 703
Bsp1286I GDGCHC 1 cut(s) 554
Bsp1407I TGTACA 1 cut(s) 570
Bsp143I GATC 4 cut(s) 722, 1432, 1477, 1701
Bsp19I CCATGG 1 cut(s) 1705
BspACI CCGC 4 cut(s) 320, 889, 1286, 1288
BspANI GGCC 3 cut(s) 1095, 1341, 1384
BspCNI CTCAG 5 cut(s) 127, 159, 577, 1420, 1585
BspDI ATCGAT 1 cut(s) 159
BspFNI CGCG 1 cut(s) 1288
BspLI GGNNCC 4 cut(s) 73, 112, 317, 1342
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 2 cut(s) 1440, 1696
BspT104I TTCGAA 1 cut(s) 703
BspT107I GGYRCC 1 cut(s) 110
BsrDI GCAATG 1 cut(s) 679
BsrGI TGTACA 1 cut(s) 570
BsrI ACTGG 1 cut(s) 338
BssECI CCNNGG 3 cut(s) 263, 1286, 1705
BssMI GATC 4 cut(s) 722, 1432, 1477, 1701
BssNAI GTATAC 1 cut(s) 511
BssT1I CCWWGG 2 cut(s) 263, 1705
Bst1107I GTATAC 1 cut(s) 511
Bst2UI CCWGG 1 cut(s) 299
Bst4CI ACNGT 5 cut(s) 98, 463, 623, 1110, 1148
Bst6I CTCTTC 1 cut(s) 944
BstAPI GCANNNNNTGC 1 cut(s) 1532
BstAUI TGTACA 1 cut(s) 570
BstBI TTCGAA 1 cut(s) 703
BstC8I GCNNGC 5 cut(s) 811, 988, 1010, 1062, 1505
BstDEI CTNAG 6 cut(s) 135, 167, 555, 585, 1428, 1593
BstDSI CCRYGG 2 cut(s) 1286, 1705
BstENI CCTNNNNNAGG 1 cut(s) 540
BstF5I GGATG 4 cut(s) 859, 904, 961, 1026
BstFNI CGCG 1 cut(s) 1288
BstHHI GCGC 1 cut(s) 986
BstKTI GATC 4 cut(s) 725, 1435, 1480, 1704
BstMAI GTCTC 1 cut(s) 1184
BstMBI GATC 4 cut(s) 722, 1432, 1477, 1701
BstMWI GCNNNNNNNGC 1 cut(s) 1532
BstNI CCWGG 1 cut(s) 299
BstNSI RCATGY 1 cut(s) 1579
BstSCI CCNGG 2 cut(s) 103, 297
BstSLI GKGCMC 1 cut(s) 554
BstUI CGCG 1 cut(s) 1288
BstV1I GCAGC 4 cut(s) 28, 278, 281, 898
BstV2I GAAGAC 1 cut(s) 132
BstX2I RGATCY 1 cut(s) 722
BstYI RGATCY 1 cut(s) 722
BstZ17I GTATAC 1 cut(s) 511
Bsu15I ATCGAT 1 cut(s) 159
Bsu36I CCTNAGG 1 cut(s) 1428
BsuRI GGCC 3 cut(s) 1095, 1341, 1384
BsuTUI ATCGAT 1 cut(s) 159
BtgI CCRYGG 2 cut(s) 1286, 1705
BtsCI GGATG 4 cut(s) 859, 904, 961, 1026
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 1 cut(s) 73
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 5 cut(s) 811, 988, 1010, 1062, 1505
CfoI GCGC 1 cut(s) 986
Cfr13I GGNCC 5 cut(s) 71, 479, 1340, 1709, 1782
Cfr42I CCGCGG 1 cut(s) 1289
ClaI ATCGAT 1 cut(s) 159
Csp6I GTAC 1 cut(s) 571
CviQI GTAC 1 cut(s) 571
DdeI CTNAG 6 cut(s) 135, 167, 555, 585, 1428, 1593
DpnI GATC 4 cut(s) 724, 1434, 1479, 1703
DpnII GATC 4 cut(s) 722, 1432, 1477, 1701
DraI TTTAAA 2 cut(s) 493, 738
DriI GACNNNNNGTC 1 cut(s) 471
Eam1104I CTCTTC 1 cut(s) 944
Eam1105I GACNNNNNGTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 944
Eco130I CCWWGG 2 cut(s) 263, 1705
Eco32I GATATC 2 cut(s) 894, 962
Eco47I GGWCC 4 cut(s) 71, 479, 1709, 1782
Eco81I CCTNAGG 1 cut(s) 1428
EcoNI CCTNNNNNAGG 1 cut(s) 540
EcoO109I RGGNCCY 1 cut(s) 1782
EcoRI GAATTC 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 297
EcoRV GATATC 2 cut(s) 894, 962
EcoT14I CCWWGG 2 cut(s) 263, 1705
EcoT22I ATGCAT 2 cut(s) 121, 232
ErhI CCWWGG 2 cut(s) 263, 1705
FaqI GGGAC 1 cut(s) 57
FauI CCCGC 1 cut(s) 327
FauNDI CATATG 2 cut(s) 184, 1205
FblI GTMKAC 3 cut(s) 100, 146, 510
Fnu4HI GCNGC 4 cut(s) 42, 292, 295, 887
FokI GGATG 4 cut(s) 866, 891, 968, 1033
Fsp4HI GCNGC 4 cut(s) 42, 292, 295, 887
FspAI RTGCGCAY 1 cut(s) 985
FspBI CTAG 1 cut(s) 645
FspI TGCGCA 1 cut(s) 985
GlaI GCGC 1 cut(s) 985
GluI GCNGC 4 cut(s) 42, 292, 295, 887
GsuI CTGGAG 2 cut(s) 321, 522
HaeIII GGCC 3 cut(s) 1095, 1341, 1384
HapII CCGG 2 cut(s) 104, 278
HhaI GCGC 1 cut(s) 986
Hin6I GCGC 1 cut(s) 984
HinP1I GCGC 1 cut(s) 984
HincII GTYRAC 3 cut(s) 94, 619, 1621
HindII GTYRAC 3 cut(s) 94, 619, 1621
HpaI GTTAAC 1 cut(s) 94
HpaII CCGG 2 cut(s) 104, 278
HphI GGTGA 3 cut(s) 475, 836, 1247
Hpy166II GTNNAC 8 cut(s) 94, 101, 147, 503, 511, 527, 619, 1621
Hpy188I TCNGA 7 cut(s) 52, 168, 197, 309, 408, 634, 1332
Hpy188III TCNNGA 5 cut(s) 23, 539, 752, 897, 1785
Hpy8I GTNNAC 8 cut(s) 94, 101, 147, 503, 511, 527, 619, 1621
HpyAV CCTTC 4 cut(s) 296, 536, 1510, 1654
HpyCH4III ACNGT 5 cut(s) 98, 463, 623, 1110, 1148
HpyCH4IV ACGT 2 cut(s) 210, 1491
HpyF10VI GCNNNNNNNGC 1 cut(s) 1532
HpyF3I CTNAG 6 cut(s) 135, 167, 555, 585, 1428, 1593
HpySE526I ACGT 2 cut(s) 210, 1491
HspAI GCGC 1 cut(s) 984
KspAI GTTAAC 1 cut(s) 94
KspI CCGCGG 1 cut(s) 1289
Kzo9I GATC 4 cut(s) 722, 1432, 1477, 1701
LmnI GCTCC 5 cut(s) 321, 340, 443, 1185, 1741
Lsp1109I GCAGC 4 cut(s) 28, 278, 281, 898
LweI GCATC 6 cut(s) 53, 128, 151, 1111, 1201, 1303
MaeI CTAG 1 cut(s) 645
MaeII ACGT 2 cut(s) 210, 1491
MaeIII GTNAC 4 cut(s) 463, 640, 824, 1487
MalI GATC 4 cut(s) 724, 1434, 1479, 1703
MboI GATC 4 cut(s) 722, 1432, 1477, 1701
MboII GAAGA 4 cut(s) 132, 931, 1065, 1151
MfeI CAATTG 2 cut(s) 566, 1539
MflI RGATCY 1 cut(s) 722
MhlI GDGCHC 1 cut(s) 554
MlyI GAGTC 3 cut(s) 146, 257, 623
MmeI TCCRAC 4 cut(s) 175, 287, 1310, 1694
MnlI CCTC 8 cut(s) 19, 85, 146, 546, 946, 1085, 1423, 1575
Mph1103I ATGCAT 2 cut(s) 121, 232
MroXI GAANNNNTTC 1 cut(s) 681
MseI TTAA 5 cut(s) 93, 492, 737, 1461, 1494
MslI CAYNNNNRTG 3 cut(s) 1059, 1233, 1308
MspA1I CMGCKG 3 cut(s) 41, 889, 1288
MspI CCGG 2 cut(s) 104, 278
MspR9I CCNGG 2 cut(s) 105, 299
MssI GTTTAAAC 1 cut(s) 738
MunI CAATTG 2 cut(s) 566, 1539
Mva1269I GAATGC 1 cut(s) 88
MvaI CCWGG 1 cut(s) 299
MvnI CGCG 1 cut(s) 1288
MwoI GCNNNNNNNGC 1 cut(s) 1532
NciI CCSGG 1 cut(s) 105
NcoI CCATGG 1 cut(s) 1705
NdeI CATATG 2 cut(s) 184, 1205
NdeII GATC 4 cut(s) 722, 1432, 1477, 1701
NlaIV GGNNCC 4 cut(s) 73, 112, 317, 1342
NmuCI GTSAC 2 cut(s) 463, 824
NsbI TGCGCA 1 cut(s) 985
NsiI ATGCAT 2 cut(s) 121, 232
NspI RCATGY 1 cut(s) 1579
NspV TTCGAA 1 cut(s) 703
PciI ACATGT 1 cut(s) 1575
PcsI WCGNNNNNNNCGW 1 cut(s) 700
PctI GAATGC 1 cut(s) 88
PdmI GAANNNNTTC 1 cut(s) 681
PfeI GAWTC 7 cut(s) 156, 362, 1003, 1070, 1628, 1690, 1726
PflMI CCANNNNNTGG 2 cut(s) 269, 520
PkrI GCNGC 4 cut(s) 43, 293, 296, 888
PleI GAGTC 3 cut(s) 145, 256, 623
PmeI GTTTAAAC 1 cut(s) 738
PpsI GAGTC 3 cut(s) 145, 256, 623
PpuMI RGGWCCY 1 cut(s) 1782
PscI ACATGT 1 cut(s) 1575
PsiI TTATAA 1 cut(s) 414
Psp1406I AACGTT 1 cut(s) 210
Psp5II RGGWCCY 1 cut(s) 1782
Psp6I CCWGG 1 cut(s) 297
PspGI CCWGG 1 cut(s) 297
PspN4I GGNNCC 4 cut(s) 73, 112, 317, 1342
PspPI GGNCC 5 cut(s) 71, 479, 1340, 1709, 1782
PspPPI RGGWCCY 1 cut(s) 1782
PsrI GAACNNNNNNTAC 2 cut(s) 1289, 1321
PsuI RGATCY 1 cut(s) 722
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
RseI CAYNNNNRTG 3 cut(s) 1059, 1233, 1308
SacII CCGCGG 1 cut(s) 1289
SaqAI TTAA 5 cut(s) 93, 492, 737, 1461, 1494
SatI GCNGC 4 cut(s) 42, 292, 295, 887
Sau3AI GATC 4 cut(s) 722, 1432, 1477, 1701
Sau96I GGNCC 5 cut(s) 71, 479, 1340, 1709, 1782
SchI GAGTC 3 cut(s) 146, 257, 623
ScrFI CCNGG 2 cut(s) 105, 299
SduI GDGCHC 1 cut(s) 554
SfaNI GCATC 6 cut(s) 53, 128, 151, 1111, 1201, 1303
Sfr303I CCGCGG 1 cut(s) 1289
SfuI TTCGAA 1 cut(s) 703
SgrBI CCGCGG 1 cut(s) 1289
SinI GGWCC 4 cut(s) 71, 479, 1709, 1782
SmiMI CAYNNNNRTG 3 cut(s) 1059, 1233, 1308
SsiI CCGC 4 cut(s) 320, 889, 1286, 1288
SspI AATATT 1 cut(s) 403
SspMI CTAG 1 cut(s) 645
StyD4I CCNGG 2 cut(s) 103, 297
StyI CCWWGG 2 cut(s) 263, 1705
TaaI ACNGT 5 cut(s) 98, 463, 623, 1110, 1148
TaiI ACGT 2 cut(s) 213, 1494
TaqI TCGA 5 cut(s) 7, 159, 604, 703, 864
TatI WGTACW 1 cut(s) 570
TfiI GAWTC 7 cut(s) 156, 362, 1003, 1070, 1628, 1690, 1726
Tru1I TTAA 5 cut(s) 93, 492, 737, 1461, 1494
Tru9I TTAA 5 cut(s) 93, 492, 737, 1461, 1494
TscAI CASTG 1 cut(s) 73
TseFI GTSAC 2 cut(s) 463, 824
TseI GCWGC 4 cut(s) 41, 291, 294, 886
Tsp45I GTSAC 2 cut(s) 463, 824
TspRI CASTG 1 cut(s) 73
Van91I CCANNNNNTGG 2 cut(s) 269, 520
VpaK11BI GGWCC 4 cut(s) 71, 479, 1709, 1782
XagI CCTNNNNNAGG 1 cut(s) 540
XapI RAATTY 5 cut(s) 18, 31, 254, 911, 1455
XceI RCATGY 1 cut(s) 1579
XcmI CCANNNNNNNNNTGG 1 cut(s) 1712
XmiI GTMKAC 3 cut(s) 100, 146, 510
XmnI GAANNNNTTC 1 cut(s) 681
XspI CTAG 1 cut(s) 645
Zsp2I ATGCAT 2 cut(s) 121, 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.