Rorug06G0008800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001547
Physical Location & Seq
Forward (+)
1357687 .. 1358706
1020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0008800.1

Sequence Viewer

Length: 1020 bp
ATGGAGGTGAAGAATCGAGTACTCTCTGGGGGTCCTTGGTTCTATAACAACTCCATGTTGTTGTTGGCAGATTATGATGGAATCTCTGCCCTCGATACGGCGCCGCTGCATCTCCTTGAGGTGTGGGTGGCTGTGAAAGGGTTACAGATTGCAATGCGAAATGAGAAAGCTCTTACTCTAATCGGACGGGCTTTGGGGGACTTTGTGCGGGTTGACCAAGGAGCGGTGCTGAGAAAGGATCCTGTCCAGAGGATCCGTGTGATTCAAGATGTCCGCCGGAGGATTTGGCCGCGACGGATGTTTGAGTTTTCACCGGTTGTTTCGGTGACGGTGGAGCTGCAGCATGAGAAATGCCATGGGCTGTGTGCGGCTTGTGGTTTCTTCGGCCATGGGGGTGGTTCTTGTGACAGGAGGCTGGCGGAGGAGGCGCCCTTGTTGGCGGTGCCGGGCCGAGGTGACTTGAGCCGTGGTCTAGAATCTGCATCTGGATCGGAAGCATCTGTGGCGGCGGTCGTGGGGCAGGAGGTGCTCTTTAAGGCGCCGACGGAGCTGGGTCTGGTGACGGCGGGGGCTGGAGAGGCTGTGGTGGCTGAGGGTGGACCGGCGTCGGGGGCTGCAATCAGTGCAGCTGCAGCTCCGGAAAAACCAGCGGGAAACCCTACTTTTGAATTAGGGTTGACCGCAGGTTTGACTGGTATGCAATCTGGGCTGCAGTCTGTTGGGCCGGAAAAGGAGGGGTCAGATGGGCTTTCTGTTGGGCTTGCTACTGGAAAAAGCCCAAAACTGGGTTTGGGTGTGAGAAAGAAGGTGTTTAAGCCTAGTTTGGCATTTATACCTCCGGAATTTCACCTTGGTGAGTTGGTGGAGGTTCCAATTTCGATGGGGACAGCTCCAAAGAAGAAGGGTCGTCCGTATGTACGTCGAGCAAAACGTATATTATCTCCAGAGAAAGCGGAAGGTAATAATGCAGGTGTGGATTTATGCTCCAACTCGAATGAGAAGGAGCTATTTATTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

339

Amino Acids

35.67

Weight (kDa)

8.76

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 959
Acc36I ACCTGC 2 cut(s) 674, 959
AccB1I GGYRCC 4 cut(s) 100, 427, 442, 538
AccBSI CCGCTC 1 cut(s) 224
AccII CGCG 1 cut(s) 292
AccIII TCCGGA 2 cut(s) 637, 838
AclWI GGATC 5 cut(s) 233, 246, 247, 260, 496
AcoI YGGCCR 2 cut(s) 287, 385
AcsI RAATTY 1 cut(s) 842
AcyI GRCGYC 4 cut(s) 101, 428, 539, 605
AfaI GTAC 2 cut(s) 21, 918
AfiI CCNNNNNNNGG 6 cut(s) 97, 223, 452, 608, 784, 785
AgeI ACCGGT 1 cut(s) 313
AgsI TTSAA 2 cut(s) 266, 668
AjuI GAANNNNNNNTTGG 2 cut(s) 834, 866
AleI CACNNNNGTG 1 cut(s) 852
AluBI AGCT 7 cut(s) 170, 337, 550, 629, 635, 890, 1006
AluI AGCT 7 cut(s) 170, 337, 550, 629, 635, 890, 1006
Alw21I GWGCWC 1 cut(s) 531
AlwI GGATC 5 cut(s) 233, 246, 247, 260, 496
Aor13HI TCCGGA 2 cut(s) 637, 838
AoxI GGCC 4 cut(s) 287, 385, 448, 722
ApeKI GCWGC 8 cut(s) 106, 337, 340, 614, 626, 629, 632, 709
ApoI RAATTY 1 cut(s) 842
ArsI GACNNNNNNTTYG 2 cut(s) 670, 702
AsiGI ACCGGT 1 cut(s) 313
AspLEI GCGC 3 cut(s) 103, 430, 541
AspS9I GGNCC 4 cut(s) 32, 448, 599, 722
AsuC2I CCSGG 1 cut(s) 447
AsuHPI GGTGA 7 cut(s) 19, 303, 337, 467, 571, 839, 866
AvaII GGWCC 2 cut(s) 32, 599
BamHI GGATCC 2 cut(s) 238, 252
BanI GGYRCC 4 cut(s) 100, 427, 442, 538
Bbv12I GWGCWC 1 cut(s) 531
BbvCI CCTCAGC 1 cut(s) 591
BbvI GCAGC 8 cut(s) 93, 324, 352, 601, 616, 638, 644, 696
BccI CCATC 3 cut(s) 71, 737, 874
BceAI ACGGC 3 cut(s) 114, 450, 579
BcgI CGANNNNNNTGC 2 cut(s) 471, 505
BcnI CCSGG 1 cut(s) 447
BfaI CTAG 2 cut(s) 473, 819
BfmI CTRYAG 3 cut(s) 338, 630, 710
BfoI RGCGCY 3 cut(s) 104, 431, 542
BfuAI ACCTGC 2 cut(s) 674, 959
BmcAI AGTACT 1 cut(s) 21
Bme1390I CCNGG 1 cut(s) 447
Bme18I GGWCC 2 cut(s) 32, 599
BmgT120I GGNCC 4 cut(s) 32, 448, 599, 722
BmiI GGNNCC 8 cut(s) 33, 102, 240, 254, 429, 444, 540, 870
BmrFI CCNGG 1 cut(s) 447
BmrI ACTGGG 1 cut(s) 794
BmsI GCATC 3 cut(s) 118, 491, 506
BmuI ACTGGG 1 cut(s) 794
BoxI GACNNNNGTC 1 cut(s) 604
BpmI CTGGAG 2 cut(s) 594, 927
Bpu10I CCTNAGC 1 cut(s) 591
BpuEI CTTGAG 2 cut(s) 137, 481
BpuMI CCSGG 1 cut(s) 447
BsaHI GRCGYC 4 cut(s) 101, 428, 539, 605
BsaJI CCNNGG 7 cut(s) 35, 217, 355, 388, 451, 466, 850
BsaWI WCCGGW 3 cut(s) 313, 637, 838
BsaXI ACNNNNNCTCC 2 cut(s) 567, 597
Bsc4I CCNNNNNNNGG 6 cut(s) 97, 223, 452, 608, 784, 785
Bse118I RCCGGY 2 cut(s) 313, 601
Bse1I ACTGG 3 cut(s) 697, 772, 789
Bse3DI GCAATG 1 cut(s) 159
BseAI TCCGGA 2 cut(s) 637, 838
BseDI CCNNGG 7 cut(s) 35, 217, 355, 388, 451, 466, 850
BseGI GGATG 1 cut(s) 303
BseLI CCNNNNNNNGG 6 cut(s) 97, 223, 452, 608, 784, 785
BseMI GCAATG 1 cut(s) 159
BseMII CTCAG 2 cut(s) 221, 582
BseNI ACTGG 3 cut(s) 697, 772, 789
BseRI GAGGAG 1 cut(s) 437
BseXI GCAGC 8 cut(s) 93, 324, 352, 601, 616, 638, 644, 696
BseYI CCCAGC 1 cut(s) 550
BsgI GTGCAG 1 cut(s) 645
Bsh1236I CGCG 1 cut(s) 292
Bsh1285I CGRYCG 1 cut(s) 513
BshFI GGCC 4 cut(s) 289, 387, 450, 724
BshNI GGYRCC 4 cut(s) 100, 427, 442, 538
BshTI ACCGGT 1 cut(s) 313
BsiEI CGRYCG 1 cut(s) 513
BsiHKAI GWGCWC 1 cut(s) 531
BsiSI CCGG 7 cut(s) 277, 314, 446, 602, 638, 725, 839
BslFI GGGAC 2 cut(s) 212, 898
BslI CCNNNNNNNGG 6 cut(s) 97, 223, 452, 608, 784, 785
BsmFI GGGAC 2 cut(s) 212, 898
BsnI GGCC 4 cut(s) 289, 387, 450, 724
Bsp1286I GDGCHC 1 cut(s) 531
Bsp13I TCCGGA 2 cut(s) 637, 838
Bsp143I GATC 3 cut(s) 238, 252, 488
Bsp19I CCATGG 2 cut(s) 355, 388
BspANI GGCC 4 cut(s) 289, 387, 450, 724
BspCNI CTCAG 2 cut(s) 222, 583
BspEI TCCGGA 2 cut(s) 637, 838
BspFNI CGCG 1 cut(s) 292
BspLI GGNNCC 8 cut(s) 33, 102, 240, 254, 429, 444, 540, 870
BspMAI CTGCAG 3 cut(s) 342, 634, 714
BspMI ACCTGC 2 cut(s) 674, 959
BspPI GGATC 5 cut(s) 233, 246, 247, 260, 496
BspT107I GGYRCC 4 cut(s) 100, 427, 442, 538
BsrBI CCGCTC 1 cut(s) 224
BsrDI GCAATG 1 cut(s) 159
BsrFI RCCGGY 2 cut(s) 313, 601
BsrI ACTGG 3 cut(s) 697, 772, 789
BssAI RCCGGY 2 cut(s) 313, 601
BssECI CCNNGG 7 cut(s) 35, 217, 355, 388, 451, 466, 850
BssMI GATC 3 cut(s) 238, 252, 488
BssNI GRCGYC 4 cut(s) 101, 428, 539, 605
BssT1I CCWWGG 5 cut(s) 35, 217, 355, 388, 850
Bst4CI ACNGT 1 cut(s) 331
BstACI GRCGYC 4 cut(s) 101, 428, 539, 605
BstAPI GCANNNNNTGC 2 cut(s) 526, 623
BstC8I GCNNGC 2 cut(s) 417, 762
BstDEI CTNAG 2 cut(s) 230, 591
BstDSI CCRYGG 3 cut(s) 355, 388, 466
BstF5I GGATG 1 cut(s) 303
BstFNI CGCG 1 cut(s) 292
BstH2I RGCGCY 3 cut(s) 104, 431, 542
BstHHI GCGC 3 cut(s) 103, 430, 541
BstKTI GATC 3 cut(s) 241, 255, 491
BstMBI GATC 3 cut(s) 238, 252, 488
BstMCI CGRYCG 1 cut(s) 513
BstPAI GACNNNNGTC 1 cut(s) 604
BstSCI CCNGG 1 cut(s) 445
BstSFI CTRYAG 3 cut(s) 338, 630, 710
BstUI CGCG 1 cut(s) 292
BstV1I GCAGC 8 cut(s) 93, 324, 352, 601, 616, 638, 644, 696
BstX2I RGATCY 2 cut(s) 238, 252
BstXI CCANNNNNNTGG 1 cut(s) 395
BstYI RGATCY 2 cut(s) 238, 252
BsuRI GGCC 4 cut(s) 289, 387, 450, 724
BtgI CCRYGG 3 cut(s) 355, 388, 466
BtsCI GGATG 1 cut(s) 303
BtsIMutI CAGTG 1 cut(s) 628
BveI ACCTGC 2 cut(s) 674, 959
Cac8I GCNNGC 2 cut(s) 417, 762
CfoI GCGC 3 cut(s) 103, 430, 541
Cfr10I RCCGGY 2 cut(s) 313, 601
Cfr13I GGNCC 4 cut(s) 32, 448, 599, 722
CseI GACGC 1 cut(s) 594
Csp6I GTAC 2 cut(s) 20, 917
CspAI ACCGGT 1 cut(s) 313
CviAII CATG 4 cut(s) 55, 344, 356, 389
CviQI GTAC 2 cut(s) 20, 917
DdeI CTNAG 2 cut(s) 230, 591
DinI GGCGCC 3 cut(s) 102, 429, 540
DpnI GATC 3 cut(s) 240, 254, 490
DpnII GATC 3 cut(s) 238, 252, 488
EaeI YGGCCR 2 cut(s) 287, 385
EciI GGCGGA 2 cut(s) 263, 434
Eco130I CCWWGG 5 cut(s) 35, 217, 355, 388, 850
Eco47I GGWCC 2 cut(s) 32, 599
EcoO109I RGGNCCY 1 cut(s) 32
EcoT14I CCWWGG 5 cut(s) 35, 217, 355, 388, 850
EgeI GGCGCC 3 cut(s) 102, 429, 540
EheI GGCGCC 3 cut(s) 102, 429, 540
ErhI CCWWGG 5 cut(s) 35, 217, 355, 388, 850
FaeI CATG 4 cut(s) 58, 347, 359, 392
FaqI GGGAC 2 cut(s) 212, 898
FatI CATG 4 cut(s) 54, 343, 355, 388
FauI CCCGC 3 cut(s) 201, 559, 643
FokI GGATG 1 cut(s) 310
FspBI CTAG 2 cut(s) 473, 819
GlaI GCGC 3 cut(s) 102, 429, 540
GsaI CCCAGC 1 cut(s) 554
GsuI CTGGAG 2 cut(s) 594, 927
HaeII RGCGCY 3 cut(s) 104, 431, 542
HaeIII GGCC 4 cut(s) 289, 387, 450, 724
HapII CCGG 7 cut(s) 277, 314, 446, 602, 638, 725, 839
HgaI GACGC 1 cut(s) 594
HhaI GCGC 3 cut(s) 103, 430, 541
Hin1I GRCGYC 4 cut(s) 101, 428, 539, 605
Hin1II CATG 4 cut(s) 58, 347, 359, 392
Hin6I GCGC 3 cut(s) 101, 428, 539
HinP1I GCGC 3 cut(s) 101, 428, 539
HincII GTYRAC 2 cut(s) 214, 678
HindII GTYRAC 2 cut(s) 214, 678
HinfI GANTC 4 cut(s) 13, 81, 262, 476
HpaII CCGG 7 cut(s) 277, 314, 446, 602, 638, 725, 839
HphI GGTGA 7 cut(s) 19, 303, 337, 467, 571, 839, 866
Hpy166II GTNNAC 3 cut(s) 214, 599, 678
Hpy188I TCNGA 3 cut(s) 185, 493, 742
Hpy188III TCNNGA 7 cut(s) 247, 266, 473, 486, 638, 839, 944
Hpy8I GTNNAC 3 cut(s) 214, 599, 678
Hpy99I CGWCG 4 cut(s) 297, 547, 610, 924
HpyAV CCTTC 4 cut(s) 799, 895, 950, 994
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4IV ACGT 2 cut(s) 919, 931
HpyF3I CTNAG 2 cut(s) 230, 591
HpySE526I ACGT 2 cut(s) 919, 931
Hsp92I GRCGYC 4 cut(s) 101, 428, 539, 605
Hsp92II CATG 4 cut(s) 58, 347, 359, 392
HspAI GCGC 3 cut(s) 101, 428, 539
KasI GGCGCC 3 cut(s) 100, 427, 538
Kpn2I TCCGGA 2 cut(s) 637, 838
Kzo9I GATC 3 cut(s) 238, 252, 488
LmnI GCTCC 7 cut(s) 221, 334, 547, 640, 895, 989, 1003
Lsp1109I GCAGC 8 cut(s) 93, 324, 352, 601, 616, 638, 644, 696
LweI GCATC 3 cut(s) 118, 491, 506
MaeI CTAG 2 cut(s) 473, 819
MaeII ACGT 2 cut(s) 919, 931
MaeIII GTNAC 5 cut(s) 141, 325, 404, 455, 559
MalI GATC 3 cut(s) 240, 254, 490
MbiI CCGCTC 1 cut(s) 224
MboI GATC 3 cut(s) 238, 252, 488
MboII GAAGA 3 cut(s) 22, 373, 910
MflI RGATCY 2 cut(s) 238, 252
MhlI GDGCHC 1 cut(s) 531
MluCI AATT 3 cut(s) 668, 842, 873
Mly113I GGCGCC 3 cut(s) 101, 428, 539
MmeI TCCRAC 1 cut(s) 1011
MroI TCCGGA 2 cut(s) 637, 838
MseI TTAA 3 cut(s) 534, 813, 1018
MslI CAYNNNNRTG 2 cut(s) 393, 852
MspA1I CMGCKG 3 cut(s) 106, 629, 650
MspI CCGG 7 cut(s) 277, 314, 446, 602, 638, 725, 839
MspR9I CCNGG 1 cut(s) 447
MvnI CGCG 1 cut(s) 292
NarI GGCGCC 3 cut(s) 101, 428, 539
NciI CCSGG 1 cut(s) 447
NcoI CCATGG 2 cut(s) 355, 388
NdeII GATC 3 cut(s) 238, 252, 488
NlaIII CATG 4 cut(s) 58, 347, 359, 392
NlaIV GGNNCC 8 cut(s) 33, 102, 240, 254, 429, 444, 540, 870
NmeAIII GCCGAG 1 cut(s) 476
NmuCI GTSAC 4 cut(s) 325, 404, 455, 559
OliI CACNNNNGTG 1 cut(s) 852
PaqCI CACCTGC 1 cut(s) 959
PcsI WCGNNNNNNNCGW 1 cut(s) 928
PfeI GAWTC 4 cut(s) 13, 81, 262, 476
PinAI ACCGGT 1 cut(s) 313
PluTI GGCGCC 3 cut(s) 104, 431, 542
PpuMI RGGWCCY 1 cut(s) 32
PshAI GACNNNNGTC 1 cut(s) 604
Psp5II RGGWCCY 1 cut(s) 32
PspFI CCCAGC 1 cut(s) 550
PspN4I GGNNCC 8 cut(s) 33, 102, 240, 254, 429, 444, 540, 870
PspPI GGNCC 4 cut(s) 32, 448, 599, 722
PspPPI RGGWCCY 1 cut(s) 32
PstI CTGCAG 3 cut(s) 342, 634, 714
PsuI RGATCY 2 cut(s) 238, 252
PvuII CAGCTG 1 cut(s) 629
RsaI GTAC 2 cut(s) 21, 918
RsaNI GTAC 2 cut(s) 20, 917
RseI CAYNNNNRTG 2 cut(s) 393, 852
SaqAI TTAA 3 cut(s) 534, 813, 1018
Sau3AI GATC 3 cut(s) 238, 252, 488
Sau96I GGNCC 4 cut(s) 32, 448, 599, 722
ScaI AGTACT 1 cut(s) 21
ScrFI CCNGG 1 cut(s) 447
SduI GDGCHC 1 cut(s) 531
SfaNI GCATC 3 cut(s) 118, 491, 506
SfcI CTRYAG 3 cut(s) 338, 630, 710
SfoI GGCGCC 3 cut(s) 102, 429, 540
SinI GGWCC 2 cut(s) 32, 599
SmiMI CAYNNNNRTG 2 cut(s) 393, 852
SmlI CTYRAG 2 cut(s) 116, 460
SmoI CTYRAG 2 cut(s) 116, 460
Sse9I AATT 3 cut(s) 668, 842, 873
SspDI GGCGCC 3 cut(s) 100, 427, 538
SspMI CTAG 2 cut(s) 473, 819
StyD4I CCNGG 1 cut(s) 445
StyI CCWWGG 5 cut(s) 35, 217, 355, 388, 850
TaaI ACNGT 1 cut(s) 331
TaiI ACGT 2 cut(s) 922, 934
TaqI TCGA 5 cut(s) 16, 93, 878, 922, 992
TasI AATT 3 cut(s) 668, 842, 873
TatI WGTACW 1 cut(s) 19
TauI GCSGC 4 cut(s) 106, 292, 371, 509
TfiI GAWTC 4 cut(s) 13, 81, 262, 476
Tru1I TTAA 3 cut(s) 534, 813, 1018
Tru9I TTAA 3 cut(s) 534, 813, 1018
TscAI CASTG 1 cut(s) 628
TseFI GTSAC 4 cut(s) 325, 404, 455, 559
TseI GCWGC 8 cut(s) 106, 337, 340, 614, 626, 629, 632, 709
Tsp45I GTSAC 4 cut(s) 325, 404, 455, 559
TspGWI ACGGA 4 cut(s) 245, 310, 560, 900
TspRI CASTG 1 cut(s) 628
VpaK11BI GGWCC 2 cut(s) 32, 599
XapI RAATTY 1 cut(s) 842
XbaI TCTAGA 1 cut(s) 472
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XspI CTAG 2 cut(s) 473, 819
ZrmI AGTACT 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.