pycom05g09080

G-quadruplex DNA unwinding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001547
Physical Location & Seq
Forward (+)
12125835 .. 12127070
1236 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g09080.1

Sequence Viewer

Length: 1236 bp
ATGTTTGCATTCACTTCAATGGGAGCCAAAGTTGATAACTCAATTAACAATGGATCAAGCCCTTATATTTTCAAAATCAGTGGTCAAGTTTGTCATTTAATGGGATCTCTTTTGCCTGTTGATAATGAGTCTCCAAAATTTGCTCAACTGTATGTCTATGATACATATAATGAGGTGGAAAATCGACTTAAAGTTTTTAATTCCAATGGAAACAATCAAAGCCTAGATCCAACAATTGTAGAAGGGTTAATCAAAATGTTTGATGCATCAAATGAATTGGTTAAACTTTTTCGAATGGCAAGAGATAAATTTGAAAACGATGCTCTTTCTTCATTAAAAATGACTTTGCTAGGCAGGCAATTAAATGATAGCAAACAATATGAGCAACCTACAACTGATGAAATAGGTGGTTTGATAGTTGGAGATATAGGATTGTATGATTCTAATCGAGATATTATTATAGAGTCTAAAAGTCACGAATTAAAATGTGTAACAAAGTTGAATCCTAAATTTATGTCTCTGCAATATCCAATTCTTTTTCCATATGGTGAAGATGGTTATAGACCAGATTTAAAATGGAATGAAAATTATAAAGGTAAAAAAACTAAAAGACAAAGAATACCAATGAGAGCTTTCATTGCTTATCAAATTCAAGAACGAGAACCACCATTAAGTACATTGTTAAGAGGAGGAAGACTTTTTCAACAATATTTGGTCGATTCTTTTGCAACACTTGAAGAAGATAGATTAGATTATATTAGACAAAATCAAAAAAATTTGAGAAGTGAAGTTTATAAAGGAATATATGATGCATTCTCAAAAGGTGATACTAATGCAAATAATTTGGGACAAAAAATTGTATTACCTGCTTCATATACTGGAAGTCCTAGATATGTGATAAATAATTATCAAGATGCTATGGCTATTTGTAGAGAATACGGACATCCTGATTTGTTTATAACATTTACATGTAATGTTAAATGGCCAGAAATCACAAGAGAGTTTGAGAATAAGCCAGAATTCAAACCTGAAGATAGACCTGATATAATTTCTAGAATCTTCAAAATGAAGCTTGATCATATGATTGATTTCATCAAGTCCGGGAAGCCTTTTGGAGAAATTCAAGCAAGTAAGTTACTAAAACTCATTCTAGCACTTATTTCAAGCTTTTTAGTTTTCTTATTTATTATAAAATTTACTTTTATTCTTTTCTTTGTTTTCATTTTTTCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

412

Amino Acids

47.45

Weight (kDa)

8.4

Isoelectric Point (pI)

31.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 212 - 376 5.1e-44 Helitron helicase-like domain at N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 591, 795, 959, 1190
Acc36I ACCTGC 1 cut(s) 874
AclWI GGATC 3 cut(s) 61, 112, 221
AcoI YGGCCR 1 cut(s) 983
AcsI RAATTY 8 cut(s) 137, 308, 509, 648, 775, 1019, 1119, 1193
AcuI CTGAAG 1 cut(s) 1050
AfaI GTAC 1 cut(s) 676
AflIII ACRYGT 1 cut(s) 968
AluBI AGCT 3 cut(s) 632, 1072, 1167
AluI AGCT 3 cut(s) 632, 1072, 1167
Alw26I GTCTC 2 cut(s) 135, 522
AlwI GGATC 3 cut(s) 61, 112, 221
AoxI GGCC 1 cut(s) 983
ApoI RAATTY 8 cut(s) 137, 308, 509, 648, 775, 1019, 1119, 1193
AsuC2I CCSGG 1 cut(s) 1102
AsuHPI GGTGA 2 cut(s) 560, 836
AsuII TTCGAA 1 cut(s) 292
BalI TGGCCA 1 cut(s) 985
BbsI GAAGAC 1 cut(s) 700
BccI CCATC 1 cut(s) 548
BcgI CGANNNNNNTGC 2 cut(s) 707, 741
BclI TGATCA 1 cut(s) 1075
BcnI CCSGG 1 cut(s) 1102
BcoDI GTCTC 2 cut(s) 135, 522
BfaI CTAG 5 cut(s) 224, 350, 888, 1053, 1151
BfuAI ACCTGC 1 cut(s) 874
Bme1390I CCNGG 1 cut(s) 1102
BmiI GGNNCC 1 cut(s) 25
BmrFI CCNGG 1 cut(s) 1102
BmsI GCATC 5 cut(s) 253, 275, 310, 799, 904
BpiI GAAGAC 1 cut(s) 700
Bpu14I TTCGAA 1 cut(s) 292
BpuMI CCSGG 1 cut(s) 1102
BsaBI GATNNNNATC 1 cut(s) 444
BsaXI ACNNNNNCTCC 2 cut(s) 15, 45
Bse1I ACTGG 1 cut(s) 883
Bse3DI GCAATG 1 cut(s) 636
Bse8I GATNNNNATC 1 cut(s) 444
BseGI GGATG 1 cut(s) 943
BseJI GATNNNNATC 1 cut(s) 444
BseMI GCAATG 1 cut(s) 636
BseNI ACTGG 1 cut(s) 883
BseRI GAGGAG 1 cut(s) 702
BshFI GGCC 1 cut(s) 985
BsiSI CCGG 1 cut(s) 1101
BslFI GGGAC 1 cut(s) 861
BsmAI GTCTC 2 cut(s) 135, 522
BsmFI GGGAC 1 cut(s) 861
BsmI GAATGC 2 cut(s) 8, 812
BsnI GGCC 1 cut(s) 985
Bsp119I TTCGAA 1 cut(s) 292
Bsp143I GATC 4 cut(s) 53, 104, 226, 1075
BspANI GGCC 1 cut(s) 985
BspHI TCATGA 1 cut(s) 1232
BspLI GGNNCC 1 cut(s) 25
BspMI ACCTGC 1 cut(s) 874
BspPI GGATC 3 cut(s) 61, 112, 221
BspT104I TTCGAA 1 cut(s) 292
BsrDI GCAATG 1 cut(s) 636
BsrI ACTGG 1 cut(s) 883
BssMI GATC 4 cut(s) 53, 104, 226, 1075
Bst4CI ACNGT 1 cut(s) 150
BstBI TTCGAA 1 cut(s) 292
BstC8I GCNNGC 1 cut(s) 356
BstF5I GGATG 1 cut(s) 943
BstKTI GATC 4 cut(s) 56, 107, 229, 1078
BstMAI GTCTC 2 cut(s) 135, 522
BstMBI GATC 4 cut(s) 53, 104, 226, 1075
BstMWI GCNNNNNNNGC 2 cut(s) 355, 638
BstNSI RCATGY 1 cut(s) 972
BstSCI CCNGG 1 cut(s) 1100
BstV2I GAAGAC 1 cut(s) 700
BstX2I RGATCY 2 cut(s) 104, 226
BstYI RGATCY 2 cut(s) 104, 226
BsuRI GGCC 1 cut(s) 985
BtsCI GGATG 1 cut(s) 943
BtsIMutI CAGTG 1 cut(s) 85
BveI ACCTGC 1 cut(s) 874
Cac8I GCNNGC 1 cut(s) 356
CciI TCATGA 1 cut(s) 1232
Csp6I GTAC 1 cut(s) 675
CspCI CAANNNNNGTGG 2 cut(s) 61, 96
CviAII CATG 2 cut(s) 969, 1233
CviQI GTAC 1 cut(s) 675
DpnI GATC 4 cut(s) 55, 106, 228, 1077
DpnII GATC 4 cut(s) 53, 104, 226, 1075
DraI TTTAAA 1 cut(s) 573
EaeI YGGCCR 1 cut(s) 983
Eco57I CTGAAG 1 cut(s) 1050
EcoRI GAATTC 1 cut(s) 1019
EcoT22I ATGCAT 2 cut(s) 268, 814
FaeI CATG 2 cut(s) 972, 1236
FaqI GGGAC 1 cut(s) 861
FatI CATG 2 cut(s) 968, 1232
FauNDI CATATG 2 cut(s) 544, 1080
FbaI TGATCA 1 cut(s) 1075
FokI GGATG 1 cut(s) 930
FspBI CTAG 5 cut(s) 224, 350, 888, 1053, 1151
HaeIII GGCC 1 cut(s) 985
HapII CCGG 1 cut(s) 1101
Hin1II CATG 2 cut(s) 972, 1236
HindIII AAGCTT 2 cut(s) 1070, 1165
HinfI GANTC 6 cut(s) 128, 440, 464, 502, 719, 1056
HpaII CCGG 1 cut(s) 1101
HphI GGTGA 2 cut(s) 560, 836
Hpy188III TCNNGA 7 cut(s) 449, 476, 653, 911, 947, 1053, 1233
HpyAV CCTTC 1 cut(s) 236
HpyCH4III ACNGT 1 cut(s) 150
HpyCH4V TGCA 6 cut(s) 8, 266, 523, 728, 812, 836
HpyF10VI GCNNNNNNNGC 2 cut(s) 355, 638
Hsp92II CATG 2 cut(s) 972, 1236
Ksp22I TGATCA 1 cut(s) 1075
Kzo9I GATC 4 cut(s) 53, 104, 226, 1075
LmnI GCTCC 1 cut(s) 23
LweI GCATC 5 cut(s) 253, 275, 310, 799, 904
MaeI CTAG 5 cut(s) 224, 350, 888, 1053, 1151
MaeIII GTNAC 3 cut(s) 473, 490, 1134
MalI GATC 4 cut(s) 55, 106, 228, 1077
MboI GATC 4 cut(s) 53, 104, 226, 1075
MboII GAAGA 7 cut(s) 321, 563, 705, 749, 752, 1043, 1051
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 2 cut(s) 104, 226
MlsI TGGCCA 1 cut(s) 985
MluNI TGGCCA 1 cut(s) 985
MlyI GAGTC 2 cut(s) 137, 473
MmeI TCCRAC 2 cut(s) 254, 400
MnlI CCTC 3 cut(s) 166, 680, 683
Mox20I TGGCCA 1 cut(s) 985
Mph1103I ATGCAT 2 cut(s) 268, 814
MscI TGGCCA 1 cut(s) 985
MslI CAYNNNNRTG 2 cut(s) 17, 967
Msp20I TGGCCA 1 cut(s) 985
MspI CCGG 1 cut(s) 1101
MspR9I CCNGG 1 cut(s) 1102
MunI CAATTG 1 cut(s) 234
Mva1269I GAATGC 2 cut(s) 8, 812
MwoI GCNNNNNNNGC 2 cut(s) 355, 638
NciI CCSGG 1 cut(s) 1102
NdeI CATATG 2 cut(s) 544, 1080
NdeII GATC 4 cut(s) 53, 104, 226, 1075
NlaIII CATG 2 cut(s) 972, 1236
NlaIV GGNNCC 1 cut(s) 25
NmuCI GTSAC 1 cut(s) 473
NsiI ATGCAT 2 cut(s) 268, 814
NspI RCATGY 1 cut(s) 972
NspV TTCGAA 1 cut(s) 292
PagI TCATGA 1 cut(s) 1232
PciI ACATGT 1 cut(s) 968
PctI GAATGC 2 cut(s) 8, 812
PfeI GAWTC 4 cut(s) 440, 502, 719, 1056
PfoI TCCNGGA 1 cut(s) 1100
PleI GAGTC 2 cut(s) 136, 472
PpsI GAGTC 2 cut(s) 136, 472
PscI ACATGT 1 cut(s) 968
PsiI TTATAA 4 cut(s) 591, 795, 959, 1190
PspN4I GGNNCC 1 cut(s) 25
PsuI RGATCY 2 cut(s) 104, 226
RsaI GTAC 1 cut(s) 676
RsaNI GTAC 1 cut(s) 675
RseI CAYNNNNRTG 2 cut(s) 17, 967
Sau3AI GATC 4 cut(s) 53, 104, 226, 1075
SchI GAGTC 2 cut(s) 137, 473
ScrFI CCNGG 1 cut(s) 1102
SfaNI GCATC 5 cut(s) 253, 275, 310, 799, 904
SfuI TTCGAA 1 cut(s) 292
SmiMI CAYNNNNRTG 2 cut(s) 17, 967
SspI AATATT 1 cut(s) 710
SspMI CTAG 5 cut(s) 224, 350, 888, 1053, 1151
StyD4I CCNGG 1 cut(s) 1100
TaaI ACNGT 1 cut(s) 150
TaqI TCGA 4 cut(s) 184, 292, 448, 717
TatI WGTACW 1 cut(s) 674
TfiI GAWTC 4 cut(s) 440, 502, 719, 1056
TscAI CASTG 1 cut(s) 85
TseFI GTSAC 1 cut(s) 473
Tsp45I GTSAC 1 cut(s) 473
TspDTI ATGAA 9 cut(s) 288, 321, 414, 597, 625, 861, 1081, 1082, 1210
TspGWI ACGGA 1 cut(s) 954
TspRI CASTG 1 cut(s) 85
XapI RAATTY 8 cut(s) 137, 308, 509, 648, 775, 1019, 1119, 1193
XbaI TCTAGA 1 cut(s) 1052
XceI RCATGY 1 cut(s) 972
XcmI CCANNNNNNNNNTGG 1 cut(s) 573
XspI CTAG 5 cut(s) 224, 350, 888, 1053, 1151
Zsp2I ATGCAT 2 cut(s) 268, 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.