pycom17g19080

G-quadruplex DNA unwinding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001547
Physical Location & Seq
Forward (+)
16699245 .. 16704753
5509 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g19080.1

Sequence Viewer

Length: 1341 bp
ATGTTCTCATTTACTTCAATGGGTGCCAAAGTTGATCACTCAATTAATGATGGGTCAAGCCCTTATATTTTCAAAATTAGTGGGCAAGTTTGTCATCTGATGGGATCTCTTTTACCAGCTGACAATGAGTCTCCAAAGTTTGCTCAATTGTATATTTATGATACATATAATGAAGTTCAGAATCGTCTTAGAGCTTTTGAATCTGATGGAAATAATAAAAAACTTGATCCAAAAATTGTTGAAGGTTTGATTAAAATGTTTGATGCATCAAATAAGTTAGTTAAGCTATTTCGAACAGCAAGGGATAAATATGAAACTGATGGACTAATTTCTTTAAAAATGAGAATATTAGCAAGAGAACTAACGGATAGCAAGCAATATGACCAACCAACAAGTGATGAATTAAGTGGTTTAATAGTTGGTGATATAGGACTTGCTGATTCTAATAGAGACATTATTATAGAGTCTAAAAGTGAAGGGTTAAAGCGTGTCACAAAATTAAATCCAAAATTTATGTCTTTGCAATATCCTATTCTTTTTCCATACGGTGAAGATGGATATAGACCAGATTTAAAGTGGAGTGAAAATTATAAAGGTCCAAAAACTAAAAGACAAAGAATACCCATGAGAGCTTTTATAGCTTACCAAATTCAAGAACGAGAACCATGTTTAACAACATTGTCAAAAGGAGGAAGACTTTTTCAACAATATTTGGTAGATTCTTATGCAACACTTGAAGAAGATAGGTTGGATTACATTAGACAAAACCAAAAAAATTTAAGAAGTGAGGTTTACAAAGGGATTTATGATGCGGTGGCAAAGGGTGATAACGATGCAAGTAATTTGGGCCAAAAAGTTGTGTTGCCAGCTTCATATACAGGAAGTCCTAAATATATGATTAATAATTATCATGATGCAATGGCTATTTGTCCAGAAAATGGACTTCCTGATTTGTTCATAACATTTACTTGCAATATAAAATGGCCAGAAATAGTTAGAGAATTTGAGAAAAAACCAGGATTCAAACCTGAGGATAGGCCTGATATAATTTCCCGCATGTTTAAAATGAAACTTGATGATATGCTTGACTTTATAAAATCTGGAAAGCCTTTTGGGGAAGTCGAAATGCCATCCAAATTCTTATATGATGCGCGAAAGAAATGTTGGAAACCGAGAAAACGCAAGGGATCAATTGGCAGAATAGCATATGTGCACCCTGCATCTGGTACATTGATGGCAACAACTACAAACAGATACATTCGGCTCCCACTTTTGACATTAAATCGAAGAACAGGCACCAAAAACTGGAAACTGGTTCACATTTTTCCAAAAGAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

447

Amino Acids

51.27

Weight (kDa)

9.33

Isoelectric Point (pI)

37.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 213 - 375 7.8e-41 Helitron helicase-like domain at N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 591, 1094
AccB1I GGYRCC 2 cut(s) 23, 1295
AccB7I CCANNNNNTGG 2 cut(s) 938, 1305
AccII CGCG 1 cut(s) 1153
AciI CCGC 2 cut(s) 812, 1054
AclWI GGATC 3 cut(s) 112, 221, 1195
AcoI YGGCCR 1 cut(s) 983
AcsI RAATTY 5 cut(s) 509, 648, 775, 1001, 1136
AfaI GTAC 1 cut(s) 1228
AfiI CCNNNNNNNGG 3 cut(s) 938, 1223, 1305
AgsI TTSAA 8 cut(s) 18, 73, 200, 242, 653, 704, 737, 1024
AhdI GACNNNNNGTC 1 cut(s) 127
AjnI CCWGG 1 cut(s) 1015
AjuI GAANNNNNNNTTGG 2 cut(s) 1147, 1179
AluBI AGCT 6 cut(s) 119, 194, 286, 632, 641, 869
AluI AGCT 6 cut(s) 119, 194, 286, 632, 641, 869
Alw21I GWGCWC 1 cut(s) 1215
Alw26I GTCTC 2 cut(s) 135, 444
Alw44I GTGCAC 1 cut(s) 1211
AlwI GGATC 3 cut(s) 112, 221, 1195
AoxI GGCC 3 cut(s) 847, 983, 1037
ApaLI GTGCAC 1 cut(s) 1211
ApoI RAATTY 5 cut(s) 509, 648, 775, 1001, 1136
ArsI GACNNNNNNTTYG 2 cut(s) 500, 532
AseI ATTAAT 2 cut(s) 45, 900
AspLEI GCGC 1 cut(s) 1153
AspS9I GGNCC 2 cut(s) 596, 847
AsuHPI GGTGA 3 cut(s) 434, 560, 836
AsuII TTCGAA 1 cut(s) 292
AvaII GGWCC 1 cut(s) 596
AxyI CCTNAGG 1 cut(s) 1029
BaeGI GKGCMC 1 cut(s) 1215
BalI TGGCCA 1 cut(s) 985
BanI GGYRCC 2 cut(s) 23, 1295
BbsI GAAGAC 1 cut(s) 700
Bbv12I GWGCWC 1 cut(s) 1215
BccI CCATC 7 cut(s) 44, 94, 200, 314, 548, 1138, 1228
BciT130I CCWGG 1 cut(s) 1017
BclI TGATCA 1 cut(s) 34
BcoDI GTCTC 2 cut(s) 135, 444
Bme1390I CCNGG 1 cut(s) 1017
Bme18I GGWCC 1 cut(s) 596
BmeRI GACNNNNNGTC 1 cut(s) 127
BmgT120I GGNCC 2 cut(s) 596, 847
BmiI GGNNCC 3 cut(s) 25, 1265, 1297
BmrFI CCNGG 1 cut(s) 1017
BmsI GCATC 7 cut(s) 253, 275, 799, 823, 904, 1138, 1229
BpiI GAAGAC 1 cut(s) 700
Bpu14I TTCGAA 1 cut(s) 292
Bsc4I CCNNNNNNNGG 3 cut(s) 938, 1223, 1305
Bse1I ACTGG 2 cut(s) 1310, 1317
Bse21I CCTNAGG 1 cut(s) 1029
Bse3DI GCAATG 1 cut(s) 924
BseBI CCWGG 1 cut(s) 1017
BseGI GGATG 1 cut(s) 1130
BseLI CCNNNNNNNGG 3 cut(s) 938, 1223, 1305
BseMI GCAATG 1 cut(s) 924
BseMII CTCAG 1 cut(s) 1020
BseNI ACTGG 2 cut(s) 1310, 1317
BseSI GKGCMC 1 cut(s) 1215
Bsh1236I CGCG 1 cut(s) 1153
BshFI GGCC 3 cut(s) 849, 985, 1039
BshNI GGYRCC 2 cut(s) 23, 1295
BsiHKAI GWGCWC 1 cut(s) 1215
BslI CCNNNNNNNGG 3 cut(s) 938, 1223, 1305
BsmAI GTCTC 2 cut(s) 135, 444
BsnI GGCC 3 cut(s) 849, 985, 1039
Bsp119I TTCGAA 1 cut(s) 292
Bsp1286I GDGCHC 1 cut(s) 1215
Bsp143I GATC 4 cut(s) 34, 104, 226, 1187
BspACI CCGC 2 cut(s) 812, 1054
BspANI GGCC 3 cut(s) 849, 985, 1039
BspCNI CTCAG 1 cut(s) 1021
BspFNI CGCG 1 cut(s) 1153
BspHI TCATGA 1 cut(s) 910
BspLI GGNNCC 3 cut(s) 25, 1265, 1297
BspPI GGATC 3 cut(s) 112, 221, 1195
BspT104I TTCGAA 1 cut(s) 292
BspT107I GGYRCC 2 cut(s) 23, 1295
BsrDI GCAATG 1 cut(s) 924
BsrI ACTGG 2 cut(s) 1310, 1317
BssMI GATC 4 cut(s) 34, 104, 226, 1187
Bst2UI CCWGG 1 cut(s) 1017
Bst4CI ACNGT 1 cut(s) 548
BstBI TTCGAA 1 cut(s) 292
BstC8I GCNNGC 2 cut(s) 374, 867
BstDEI CTNAG 2 cut(s) 188, 1029
BstF5I GGATG 1 cut(s) 1130
BstFNI CGCG 1 cut(s) 1153
BstHHI GCGC 1 cut(s) 1153
BstKTI GATC 4 cut(s) 37, 107, 229, 1190
BstMAI GTCTC 2 cut(s) 135, 444
BstMBI GATC 4 cut(s) 34, 104, 226, 1187
BstMWI GCNNNNNNNGC 1 cut(s) 638
BstNI CCWGG 1 cut(s) 1017
BstNSI RCATGY 1 cut(s) 1060
BstSCI CCNGG 1 cut(s) 1015
BstSLI GKGCMC 1 cut(s) 1215
BstUI CGCG 1 cut(s) 1153
BstV2I GAAGAC 1 cut(s) 700
BstX2I RGATCY 1 cut(s) 104
BstYI RGATCY 1 cut(s) 104
Bsu36I CCTNAGG 1 cut(s) 1029
BsuRI GGCC 3 cut(s) 849, 985, 1039
BtsCI GGATG 1 cut(s) 1130
Cac8I GCNNGC 2 cut(s) 374, 867
CciI TCATGA 1 cut(s) 910
CfoI GCGC 1 cut(s) 1153
Cfr13I GGNCC 2 cut(s) 596, 847
Csp6I GTAC 1 cut(s) 1227
CspCI CAANNNNNGTGG 2 cut(s) 61, 96
CviAII CATG 4 cut(s) 625, 666, 911, 1057
CviQI GTAC 1 cut(s) 1227
DdeI CTNAG 2 cut(s) 188, 1029
DpnI GATC 4 cut(s) 36, 106, 228, 1189
DpnII GATC 4 cut(s) 34, 104, 226, 1187
DraI TTTAAA 3 cut(s) 336, 573, 1063
DriI GACNNNNNGTC 1 cut(s) 127
EaeI YGGCCR 1 cut(s) 983
Eam1105I GACNNNNNGTC 1 cut(s) 127
Eco147I AGGCCT 1 cut(s) 1039
Eco47I GGWCC 1 cut(s) 596
Eco81I CCTNAGG 1 cut(s) 1029
EcoRII CCWGG 1 cut(s) 1015
EcoT22I ATGCAT 1 cut(s) 268
FaeI CATG 4 cut(s) 628, 669, 914, 1060
FatI CATG 4 cut(s) 624, 665, 910, 1056
FauI CCCGC 1 cut(s) 1061
FauNDI CATATG 1 cut(s) 1207
FbaI TGATCA 1 cut(s) 34
FokI GGATG 1 cut(s) 1117
GlaI GCGC 1 cut(s) 1152
HaeIII GGCC 3 cut(s) 849, 985, 1039
HhaI GCGC 1 cut(s) 1153
Hin1II CATG 4 cut(s) 628, 669, 914, 1060
Hin6I GCGC 1 cut(s) 1151
HinP1I GCGC 1 cut(s) 1151
HinfI GANTC 7 cut(s) 128, 181, 200, 440, 464, 719, 1020
HphI GGTGA 3 cut(s) 434, 560, 836
Hpy166II GTNNAC 3 cut(s) 793, 1213, 1318
Hpy188I TCNGA 3 cut(s) 99, 180, 205
Hpy188III TCNNGA 5 cut(s) 653, 911, 932, 947, 1101
Hpy8I GTNNAC 3 cut(s) 793, 1213, 1318
HpyAV CCTTC 2 cut(s) 236, 470
HpyCH4III ACNGT 1 cut(s) 548
HpyCH4V TGCA 8 cut(s) 266, 523, 728, 836, 917, 972, 1213, 1220
HpyF10VI GCNNNNNNNGC 1 cut(s) 638
HpyF3I CTNAG 2 cut(s) 188, 1029
Hsp92II CATG 4 cut(s) 628, 669, 914, 1060
HspAI GCGC 1 cut(s) 1151
Ksp22I TGATCA 1 cut(s) 34
Kzo9I GATC 4 cut(s) 34, 104, 226, 1187
LmnI GCTCC 1 cut(s) 1269
LweI GCATC 7 cut(s) 253, 275, 799, 823, 904, 1138, 1229
MaeIII GTNAC 1 cut(s) 490
MalI GATC 4 cut(s) 36, 106, 228, 1189
MboI GATC 4 cut(s) 34, 104, 226, 1187
MboII GAAGA 5 cut(s) 563, 705, 749, 752, 1299
MfeI CAATTG 2 cut(s) 146, 1191
MflI RGATCY 1 cut(s) 104
MhlI GDGCHC 1 cut(s) 1215
MlsI TGGCCA 1 cut(s) 985
MluNI TGGCCA 1 cut(s) 985
MlyI GAGTC 2 cut(s) 137, 473
MmeI TCCRAC 2 cut(s) 729, 1145
MnlI CCTC 3 cut(s) 683, 781, 1024
Mox20I TGGCCA 1 cut(s) 985
Mph1103I ATGCAT 1 cut(s) 268
MscI TGGCCA 1 cut(s) 985
Msp20I TGGCCA 1 cut(s) 985
MspA1I CMGCKG 1 cut(s) 119
MspR9I CCNGG 1 cut(s) 1017
MunI CAATTG 2 cut(s) 146, 1191
MvaI CCWGG 1 cut(s) 1017
MvnI CGCG 1 cut(s) 1153
MwoI GCNNNNNNNGC 1 cut(s) 638
NdeI CATATG 1 cut(s) 1207
NdeII GATC 4 cut(s) 34, 104, 226, 1187
NlaIII CATG 4 cut(s) 628, 669, 914, 1060
NlaIV GGNNCC 3 cut(s) 25, 1265, 1297
NmuCI GTSAC 1 cut(s) 490
NsiI ATGCAT 1 cut(s) 268
NspI RCATGY 1 cut(s) 1060
NspV TTCGAA 1 cut(s) 292
PagI TCATGA 1 cut(s) 910
PceI AGGCCT 1 cut(s) 1039
PfeI GAWTC 5 cut(s) 181, 200, 440, 719, 1020
PflMI CCANNNNNTGG 2 cut(s) 938, 1305
PleI GAGTC 2 cut(s) 136, 472
PpsI GAGTC 2 cut(s) 136, 472
PshBI ATTAAT 2 cut(s) 45, 900
PsiI TTATAA 2 cut(s) 591, 1094
Psp6I CCWGG 1 cut(s) 1015
PspGI CCWGG 1 cut(s) 1015
PspN4I GGNNCC 3 cut(s) 25, 1265, 1297
PspPI GGNCC 2 cut(s) 596, 847
PsuI RGATCY 1 cut(s) 104
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 1 cut(s) 1228
RsaNI GTAC 1 cut(s) 1227
Sau3AI GATC 4 cut(s) 34, 104, 226, 1187
Sau96I GGNCC 2 cut(s) 596, 847
SchI GAGTC 2 cut(s) 137, 473
ScrFI CCNGG 1 cut(s) 1017
SduI GDGCHC 1 cut(s) 1215
SfaNI GCATC 7 cut(s) 253, 275, 799, 823, 904, 1138, 1229
SfuI TTCGAA 1 cut(s) 292
SinI GGWCC 1 cut(s) 596
SseBI AGGCCT 1 cut(s) 1039
SsiI CCGC 2 cut(s) 812, 1054
SspI AATATT 2 cut(s) 348, 710
StuI AGGCCT 1 cut(s) 1039
StyD4I CCNGG 1 cut(s) 1015
TaaI ACNGT 1 cut(s) 548
TaqI TCGA 3 cut(s) 292, 1122, 1285
TfiI GAWTC 5 cut(s) 181, 200, 440, 719, 1020
TseFI GTSAC 1 cut(s) 490
Tsp45I GTSAC 1 cut(s) 490
TspDTI ATGAA 6 cut(s) 186, 327, 414, 861, 946, 1082
TspGWI ACGGA 1 cut(s) 380
Van91I CCANNNNNTGG 2 cut(s) 938, 1305
VneI GTGCAC 1 cut(s) 1211
VpaK11BI GGWCC 1 cut(s) 596
VspI ATTAAT 2 cut(s) 45, 900
XapI RAATTY 5 cut(s) 509, 648, 775, 1001, 1136
XceI RCATGY 1 cut(s) 1060
XcmI CCANNNNNNNNNTGG 1 cut(s) 573
Zsp2I ATGCAT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.