FvH4_5g26611

G-quadruplex DNA unwinding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001547
Physical Location & Seq
Forward (+)
18094613 .. 18097207
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g26611.t1

Sequence Viewer

Length: 1782 bp
ATGACTCGAAAGAAAAGAATTCGAGAGGAAAATTTGTCAGCTGCATCGTCTGATCAACATGGGTGCAGTGGTCCCTCCATGAATGCTTCTGTTAGCAGAGGACCGGATGGTGCCAATGCATCAAATGACTGTGCTAAGTCTTCATCTGCAAGAGGAATCGATGCTCTCAGTGTTGGACAATCTAATATCGGGAATGGAAGTCCGGTCTATACAGGCTGCTGCCAGGAAGGTCGGATTAGGCTCCCGCCTGTTAGACCTGCTCCAGTTTTTTTAGAATACTTGCTTGATTCCACTAATGGTCAAAAGAGTGTAGAGTTTAGAAACAATATTCGGAGTTATAACACTATGTTTGCATTTACTTCAATGGGAGCTAATGTAGACAAGACGGTATACCATCTTATGGGTTCACTTATTCCTCCAGAAGGTGAGTGCCCTAAGTTTGCACAATTATACATTTATGATACTGAGAATGAAGTTGCAAATCGACAAAAAGCTGTTGACGGTTTAGACTCGGAAATTGTTACTAGATTGATTGATATGCTTGATGAAAGCAATGAATTGGTTCAGTATTTTCGTTCTGTTCGAAATAAGTATAATGAAAGATCTTTGCGTTGCTTAAACTTGACAATTCTTGACAATGAAAGAATAGGAGATAGGGAATACGATGTGCCAAGTAGTAGTGAAGTTGCTGGCTTGATTGTTGGTGACATTGGGTTGTATGAAGCGGATAGAGATGTAGTTGTCGATACATTTGAAAATGGTTTGCAGCGAGTATCAAGATTACATCCAAAATTCATGTCATTTCAATATCCAGTACTCTTCCCTTATGGGGAGGATGGATATCATGTTGGTTTGAAATTATTCGCATGCAAAGATAATCAGAATCGCAAGCGAGAAAGGATGTCCATAAAAAATCAACCCGATATTAGGAGCGAGACTTATGAGGGCATATGTGATGCTTCAATAAGAGGACATGATGGTGAAAATGTAGGCAAAAGAATTATTTTACCAAGCACTCATACGGGAAGTCCGCGGTATATGAACAAACATTACCATGATGCTATGACTTTGTGTCGGAAGTATGGCCCTCCTGATTTGTTTATAATAGTTACTTGCAATCCAAAATGGCCTGAAATTGAAAGAGCATTATTGAACAAACATGGTAGCAAACTTGAGGATCGCCCAGATATTGTTGCAAGAATTTTTAAGATGAAACATGATGATCTTATTAGTTACGTTAAATCTGGCAAGCCATTTTTGAAGGCTATTGCAGATGTTGCGACAATTGAGTTCCAAAAACGGGGATTACCTCACTCTCACATGTTATTTTGGTTGGCTGAAAATAACAAGTGTTATTCGGCTGCTGACATTGATTCAATAATCTCTGCTGAACTACCTGATAAGCAGTCAACGTTGATAAAATATTTGTTCAAGTATATAAGCAAAGGTCCTGACCGAGCAAGAGTTGGTATTCAATCTGATTTAAATGATGAAATTGCAGCGTATTTAAACTGCAGATATATATCTCCACCTGAAGCTGCATGGAGATTGCTTGAATATCCAATTCATTCTCGGCACCCACCTGTTGAATTGTTGAAGATACATTTACCAGGACAACAAACCATTGTTTTTGGTCAAAATCAACCGTTAACCTCAGTCATTGCAAGACAAGGATTAGACGATACTATGCTCACGTCATGGTTTCATGCAAATGCAAACGCAGAGACAAAGGATATAGCAAATAGGTTGACATTTGCAGAGTTTCCTTCTGCCTTTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

594

Amino Acids

66.73

Weight (kDa)

6.77

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 295 - 442 5.6e-42 Helitron helicase-like domain at N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 339, 1103
Acc36I ACCTGC 1 cut(s) 265
AccB1I GGYRCC 2 cut(s) 110, 1575
AccB7I CCANNNNNTGG 1 cut(s) 400
AccI GTMKAC 2 cut(s) 378, 390
AccII CGCG 1 cut(s) 1033
AciI CCGC 4 cut(s) 245, 725, 1031, 1033
AclI AACGTT 1 cut(s) 1412
AclWI GGATC 1 cut(s) 1185
AcsI RAATTY 4 cut(s) 18, 31, 791, 1200
AcuI CTGAAG 1 cut(s) 1554
AfaI GTAC 1 cut(s) 816
AfiI CCNNNNNNNGG 5 cut(s) 400, 422, 829, 927, 1300
AflIII ACRYGT 1 cut(s) 1320
AhdI GACNNNNNGTC 1 cut(s) 1071
AjiI CACGTC 1 cut(s) 1695
AjnI CCWGG 2 cut(s) 222, 1609
AluBI AGCT 4 cut(s) 41, 371, 494, 1538
AluI AGCT 4 cut(s) 41, 371, 494, 1538
Alw26I GTCTC 2 cut(s) 929, 1718
AlwI GGATC 1 cut(s) 1185
AoxI GGCC 2 cut(s) 1084, 1127
ApeKI GCWGC 7 cut(s) 41, 216, 219, 766, 1361, 1499, 1538
ApoI RAATTY 4 cut(s) 18, 31, 791, 1200
ArsI GACNNNNNNTTYG 2 cut(s) 782, 814
Asp700I GAANNNNTTC 2 cut(s) 561, 860
AspS9I GGNCC 4 cut(s) 71, 101, 1085, 1448
AsuHPI GGTGA 3 cut(s) 437, 716, 992
AsuII TTCGAA 1 cut(s) 583
AvaII GGWCC 3 cut(s) 71, 101, 1448
BaeGI GKGCMC 1 cut(s) 434
BanI GGYRCC 2 cut(s) 110, 1575
BbsI GAAGAC 1 cut(s) 132
BbvI GCAGC 7 cut(s) 28, 203, 206, 778, 1348, 1511, 1525
BccI CCATC 4 cut(s) 101, 402, 830, 971
BciT130I CCWGG 2 cut(s) 224, 1611
BclI TGATCA 1 cut(s) 52
BcoDI GTCTC 2 cut(s) 929, 1718
BfaI CTAG 1 cut(s) 525
BfmI CTRYAG 1 cut(s) 1513
BfuAI ACCTGC 1 cut(s) 265
BglII AGATCT 1 cut(s) 602
BisI GCNGC 7 cut(s) 42, 217, 220, 767, 1362, 1500, 1539
BlsI GCNGC 7 cut(s) 43, 218, 221, 768, 1363, 1501, 1540
BmcAI AGTACT 1 cut(s) 816
Bme1390I CCNGG 2 cut(s) 224, 1611
Bme18I GGWCC 3 cut(s) 71, 101, 1448
BmeRI GACNNNNNGTC 1 cut(s) 1071
BmgBI CACGTC 1 cut(s) 1695
BmgT120I GGNCC 4 cut(s) 71, 101, 1085, 1448
BmiI GGNNCC 4 cut(s) 73, 112, 242, 1577
BmrFI CCNGG 2 cut(s) 224, 1611
BmsI GCATC 5 cut(s) 53, 128, 151, 946, 1048
BpiI GAAGAC 1 cut(s) 132
BpmI CTGGAG 2 cut(s) 246, 402
Bpu14I TTCGAA 1 cut(s) 583
BpuEI CTTGAG 1 cut(s) 1193
Bsa29I ATCGAT 1 cut(s) 159
BsaBI GATNNNNATC 2 cut(s) 840, 1522
BsaJI CCNNGG 1 cut(s) 1031
BsaWI WCCGGW 2 cut(s) 103, 202
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bsc4I CCNNNNNNNGG 5 cut(s) 400, 422, 829, 927, 1300
Bse1I ACTGG 2 cut(s) 263, 812
Bse3DI GCAATG 2 cut(s) 559, 1659
Bse8I GATNNNNATC 2 cut(s) 840, 1522
BseBI CCWGG 2 cut(s) 224, 1611
BseCI ATCGAT 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 1031
BseGI GGATG 4 cut(s) 112, 784, 841, 906
BseJI GATNNNNATC 2 cut(s) 840, 1522
BseLI CCNNNNNNNGG 5 cut(s) 400, 422, 829, 927, 1300
BseMI GCAATG 2 cut(s) 559, 1659
BseMII CTCAG 3 cut(s) 181, 456, 1668
BseNI ACTGG 2 cut(s) 263, 812
BseSI GKGCMC 1 cut(s) 434
BseXI GCAGC 7 cut(s) 28, 203, 206, 778, 1348, 1511, 1525
BsgI GTGCAG 1 cut(s) 85
Bsh1236I CGCG 1 cut(s) 1033
BshFI GGCC 2 cut(s) 1086, 1129
BshNI GGYRCC 2 cut(s) 110, 1575
BshVI ATCGAT 1 cut(s) 159
BsiSI CCGG 2 cut(s) 104, 203
BslFI GGGAC 1 cut(s) 57
BslI CCNNNNNNNGG 5 cut(s) 400, 422, 829, 927, 1300
BsmAI GTCTC 2 cut(s) 929, 1718
BsmFI GGGAC 1 cut(s) 57
BsmI GAATGC 1 cut(s) 88
BsnI GGCC 2 cut(s) 1086, 1129
Bsp119I TTCGAA 1 cut(s) 583
Bsp1286I GDGCHC 1 cut(s) 434
Bsp143I GATC 4 cut(s) 52, 602, 1177, 1222
BspACI CCGC 4 cut(s) 245, 725, 1031, 1033
BspANI GGCC 2 cut(s) 1086, 1129
BspCNI CTCAG 3 cut(s) 180, 457, 1667
BspDI ATCGAT 1 cut(s) 159
BspFNI CGCG 1 cut(s) 1033
BspLI GGNNCC 4 cut(s) 73, 112, 242, 1577
BspMAI CTGCAG 1 cut(s) 1517
BspMI ACCTGC 1 cut(s) 265
BspPI GGATC 1 cut(s) 1185
BspT104I TTCGAA 1 cut(s) 583
BspT107I GGYRCC 2 cut(s) 110, 1575
BsrDI GCAATG 2 cut(s) 559, 1659
BsrI ACTGG 2 cut(s) 263, 812
BssECI CCNNGG 1 cut(s) 1031
BssMI GATC 4 cut(s) 52, 602, 1177, 1222
BssNAI GTATAC 1 cut(s) 391
Bst1107I GTATAC 1 cut(s) 391
Bst2UI CCWGG 2 cut(s) 224, 1611
Bst4CI ACNGT 4 cut(s) 131, 388, 503, 1647
Bst6I CTCTTC 1 cut(s) 824
BstAPI GCANNNNNTGC 1 cut(s) 1277
BstBI TTCGAA 1 cut(s) 583
BstC8I GCNNGC 4 cut(s) 691, 868, 890, 1250
BstDEI CTNAG 5 cut(s) 135, 167, 435, 465, 1654
BstDSI CCRYGG 1 cut(s) 1031
BstENI CCTNNNNNAGG 1 cut(s) 420
BstF5I GGATG 4 cut(s) 112, 784, 841, 906
BstFNI CGCG 1 cut(s) 1033
BstKTI GATC 4 cut(s) 55, 605, 1180, 1225
BstMAI GTCTC 2 cut(s) 929, 1718
BstMBI GATC 4 cut(s) 52, 602, 1177, 1222
BstMWI GCNNNNNNNGC 1 cut(s) 1277
BstNI CCWGG 2 cut(s) 224, 1611
BstNSI RCATGY 2 cut(s) 870, 1324
BstSCI CCNGG 2 cut(s) 222, 1609
BstSFI CTRYAG 1 cut(s) 1513
BstSLI GKGCMC 1 cut(s) 434
BstUI CGCG 1 cut(s) 1033
BstV1I GCAGC 7 cut(s) 28, 203, 206, 778, 1348, 1511, 1525
BstV2I GAAGAC 1 cut(s) 132
BstX2I RGATCY 1 cut(s) 602
BstYI RGATCY 1 cut(s) 602
BstZ17I GTATAC 1 cut(s) 391
Bsu15I ATCGAT 1 cut(s) 159
BsuRI GGCC 2 cut(s) 1086, 1129
BsuTUI ATCGAT 1 cut(s) 159
BtgI CCRYGG 1 cut(s) 1031
BtrI CACGTC 1 cut(s) 1695
BtsCI GGATG 4 cut(s) 112, 784, 841, 906
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 2 cut(s) 73, 175
BveI ACCTGC 1 cut(s) 265
Cac8I GCNNGC 4 cut(s) 691, 868, 890, 1250
Cfr13I GGNCC 4 cut(s) 71, 101, 1085, 1448
Cfr42I CCGCGG 1 cut(s) 1034
ClaI ATCGAT 1 cut(s) 159
Csp6I GTAC 1 cut(s) 815
CviQI GTAC 1 cut(s) 815
DdeI CTNAG 5 cut(s) 135, 167, 435, 465, 1654
DpnI GATC 4 cut(s) 54, 604, 1179, 1224
DpnII GATC 4 cut(s) 52, 602, 1177, 1222
DraI TTTAAA 2 cut(s) 1485, 1509
DriI GACNNNNNGTC 1 cut(s) 1071
Eam1104I CTCTTC 1 cut(s) 824
Eam1105I GACNNNNNGTC 1 cut(s) 1071
EarI CTCTTC 1 cut(s) 824
Eco32I GATATC 1 cut(s) 842
Eco47I GGWCC 3 cut(s) 71, 101, 1448
Eco57I CTGAAG 1 cut(s) 1554
EcoNI CCTNNNNNAGG 1 cut(s) 420
EcoO109I RGGNCCY 1 cut(s) 1448
EcoRI GAATTC 1 cut(s) 18
EcoRII CCWGG 2 cut(s) 222, 1609
EcoRV GATATC 1 cut(s) 842
EcoT22I ATGCAT 1 cut(s) 121
FaqI GGGAC 1 cut(s) 57
FauI CCCGC 1 cut(s) 252
FauNDI CATATG 1 cut(s) 950
FbaI TGATCA 1 cut(s) 52
FblI GTMKAC 2 cut(s) 378, 390
Fnu4HI GCNGC 7 cut(s) 42, 217, 220, 767, 1362, 1500, 1539
FokI GGATG 4 cut(s) 119, 771, 848, 913
Fsp4HI GCNGC 7 cut(s) 42, 217, 220, 767, 1362, 1500, 1539
FspBI CTAG 1 cut(s) 525
GluI GCNGC 7 cut(s) 42, 217, 220, 767, 1362, 1500, 1539
GsuI CTGGAG 2 cut(s) 246, 402
HaeIII GGCC 2 cut(s) 1086, 1129
HapII CCGG 2 cut(s) 104, 203
HincII GTYRAC 4 cut(s) 499, 1410, 1650, 1749
HindII GTYRAC 4 cut(s) 499, 1410, 1650, 1749
HinfI GANTC 6 cut(s) 4, 156, 287, 509, 883, 1373
HpaI GTTAAC 1 cut(s) 1650
HpaII CCGG 2 cut(s) 104, 203
HphI GGTGA 3 cut(s) 437, 716, 992
Hpy166II GTNNAC 7 cut(s) 379, 391, 407, 499, 1410, 1650, 1749
Hpy188I TCNGA 7 cut(s) 52, 234, 333, 514, 882, 1077, 1480
Hpy188III TCNNGA 7 cut(s) 23, 190, 419, 632, 777, 1091, 1451
Hpy8I GTNNAC 7 cut(s) 379, 391, 407, 499, 1410, 1650, 1749
HpyAV CCTTC 4 cut(s) 221, 416, 1255, 1776
HpyCH4III ACNGT 4 cut(s) 131, 388, 503, 1647
HpyCH4IV ACGT 3 cut(s) 1236, 1412, 1694
HpyF10VI GCNNNNNNNGC 1 cut(s) 1277
HpyF3I CTNAG 5 cut(s) 135, 167, 435, 465, 1654
HpySE526I ACGT 3 cut(s) 1236, 1412, 1694
Ksp22I TGATCA 1 cut(s) 52
KspAI GTTAAC 1 cut(s) 1650
KspI CCGCGG 1 cut(s) 1034
Kzo9I GATC 4 cut(s) 52, 602, 1177, 1222
LmnI GCTCC 4 cut(s) 246, 265, 368, 930
Lsp1109I GCAGC 7 cut(s) 28, 203, 206, 778, 1348, 1511, 1525
LweI GCATC 5 cut(s) 53, 128, 151, 946, 1048
MaeI CTAG 1 cut(s) 525
MaeII ACGT 3 cut(s) 1236, 1412, 1694
MaeIII GTNAC 4 cut(s) 520, 704, 1108, 1232
MalI GATC 4 cut(s) 54, 604, 1179, 1224
MboI GATC 4 cut(s) 52, 602, 1177, 1222
MboII GAAGA 3 cut(s) 132, 811, 1609
MfeI CAATTG 1 cut(s) 1284
MflI RGATCY 1 cut(s) 602
MhlI GDGCHC 1 cut(s) 434
MlyI GAGTC 1 cut(s) 503
MmeI TCCRAC 3 cut(s) 154, 212, 1055
Mph1103I ATGCAT 1 cut(s) 121
MroXI GAANNNNTTC 2 cut(s) 561, 860
MseI TTAA 6 cut(s) 617, 1206, 1239, 1484, 1508, 1649
MslI CAYNNNNRTG 3 cut(s) 978, 1053, 1710
MspA1I CMGCKG 2 cut(s) 41, 1033
MspI CCGG 2 cut(s) 104, 203
MspR9I CCNGG 2 cut(s) 224, 1611
MunI CAATTG 1 cut(s) 1284
Mva1269I GAATGC 1 cut(s) 88
MvaI CCWGG 2 cut(s) 224, 1611
MvnI CGCG 1 cut(s) 1033
MwoI GCNNNNNNNGC 1 cut(s) 1277
NdeI CATATG 1 cut(s) 950
NdeII GATC 4 cut(s) 52, 602, 1177, 1222
NlaIV GGNNCC 4 cut(s) 73, 112, 242, 1577
NmeAIII GCCGAG 1 cut(s) 1552
NmuCI GTSAC 1 cut(s) 704
NsiI ATGCAT 1 cut(s) 121
NspI RCATGY 2 cut(s) 870, 1324
NspV TTCGAA 1 cut(s) 583
PaeI GCATGC 1 cut(s) 870
PciI ACATGT 1 cut(s) 1320
PcsI WCGNNNNNNNCGW 1 cut(s) 580
PctI GAATGC 1 cut(s) 88
PdmI GAANNNNTTC 2 cut(s) 561, 860
PfeI GAWTC 4 cut(s) 156, 287, 883, 1373
PflMI CCANNNNNTGG 1 cut(s) 400
PkrI GCNGC 7 cut(s) 43, 218, 221, 768, 1363, 1501, 1540
PleI GAGTC 1 cut(s) 503
PpsI GAGTC 1 cut(s) 503
PpuMI RGGWCCY 1 cut(s) 1448
PscI ACATGT 1 cut(s) 1320
PsiI TTATAA 2 cut(s) 339, 1103
Psp1406I AACGTT 1 cut(s) 1412
Psp5II RGGWCCY 1 cut(s) 1448
Psp6I CCWGG 2 cut(s) 222, 1609
PspGI CCWGG 2 cut(s) 222, 1609
PspN4I GGNNCC 4 cut(s) 73, 112, 242, 1577
PspPI GGNCC 4 cut(s) 71, 101, 1085, 1448
PspPPI RGGWCCY 1 cut(s) 1448
PsrI GAACNNNNNNTAC 2 cut(s) 1034, 1066
PstI CTGCAG 1 cut(s) 1517
PsuI RGATCY 1 cut(s) 602
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 1 cut(s) 816
RsaNI GTAC 1 cut(s) 815
RseI CAYNNNNRTG 3 cut(s) 978, 1053, 1710
SacII CCGCGG 1 cut(s) 1034
SaqAI TTAA 6 cut(s) 617, 1206, 1239, 1484, 1508, 1649
SatI GCNGC 7 cut(s) 42, 217, 220, 767, 1362, 1500, 1539
Sau3AI GATC 4 cut(s) 52, 602, 1177, 1222
Sau96I GGNCC 4 cut(s) 71, 101, 1085, 1448
ScaI AGTACT 1 cut(s) 816
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 2 cut(s) 224, 1611
SduI GDGCHC 1 cut(s) 434
SfaNI GCATC 5 cut(s) 53, 128, 151, 946, 1048
SfcI CTRYAG 1 cut(s) 1513
Sfr303I CCGCGG 1 cut(s) 1034
SfuI TTCGAA 1 cut(s) 583
SgrBI CCGCGG 1 cut(s) 1034
SinI GGWCC 3 cut(s) 71, 101, 1448
SmiI ATTTAAAT 1 cut(s) 1485
SmiMI CAYNNNNRTG 3 cut(s) 978, 1053, 1710
SmlI CTYRAG 1 cut(s) 1172
SmoI CTYRAG 1 cut(s) 1172
SphI GCATGC 1 cut(s) 870
SsiI CCGC 4 cut(s) 245, 725, 1031, 1033
SspI AATATT 2 cut(s) 328, 1424
SspMI CTAG 1 cut(s) 525
StyD4I CCNGG 2 cut(s) 222, 1609
SwaI ATTTAAAT 1 cut(s) 1485
TaaI ACNGT 4 cut(s) 131, 388, 503, 1647
TaiI ACGT 3 cut(s) 1239, 1415, 1697
TaqI TCGA 6 cut(s) 7, 22, 159, 484, 583, 744
TaqII GACCGA 1 cut(s) 1470
TatI WGTACW 1 cut(s) 814
TfiI GAWTC 4 cut(s) 156, 287, 883, 1373
Tru1I TTAA 6 cut(s) 617, 1206, 1239, 1484, 1508, 1649
Tru9I TTAA 6 cut(s) 617, 1206, 1239, 1484, 1508, 1649
TscAI CASTG 2 cut(s) 73, 175
TseFI GTSAC 1 cut(s) 704
TseI GCWGC 7 cut(s) 41, 216, 219, 766, 1361, 1499, 1538
Tsp45I GTSAC 1 cut(s) 704
TspRI CASTG 2 cut(s) 73, 175
Van91I CCANNNNNTGG 1 cut(s) 400
VpaK11BI GGWCC 3 cut(s) 71, 101, 1448
XagI CCTNNNNNAGG 1 cut(s) 420
XapI RAATTY 4 cut(s) 18, 31, 791, 1200
XceI RCATGY 2 cut(s) 870, 1324
XmiI GTMKAC 2 cut(s) 378, 390
XmnI GAANNNNTTC 2 cut(s) 561, 860
XspI CTAG 1 cut(s) 525
ZrmI AGTACT 1 cut(s) 816
Zsp2I ATGCAT 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.