Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0001547

Physical Location & Seq

Genomic Coordinates Forward (+)

3996379 .. 3998832

Gene Structure 2454 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g06520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2454 bp

ATGCTTATAAAATATTTATTCAAATATGTAAATAAAGGTTCAGATAGAGCACGTTTAGTTTTTCAAGAAAATTTGAATGATGAGATTTTAGCATACCTCAATTGTAGATATATATCACCTTATGAGGCTGTTTGGAGACTATTTCAATATTTCATACATCATAGAGAGCCTTCTGTAGAGCGATTACCAGTTCATTTACCATTAGAACAAAATGTTGTTTTTACAGCTGATCAATCACTACAGTCAATCATTAATCAAAAAGCTTTTGAAAACACAATGTTAACAAGTTGGTTTGAATTGAACAAAAATTTTCCTAACGCACGTGAACTAACTTATGCACAAATTCCCACAAAATTTTTGTATGATGTAAAGAAAAAAACTTGGAAGCCAAGAAAATATAAAGCATCAATTGGCAGAATAGCATATGTACATCCTGCATCTGGCGAATTATATTATTTGAGAATGCTTTTGAATTTAAAAAAAGGCGCCATTAGTTTTGAGGATGTAAAAACAATTAATGGTGTTGTACATTCTTCCTACCATTCTGCATGCAAATCATTAGGACTATTAGGTGATGATAAAGAATGGATTGAGGCTTTATCAAGTGCTGCACATATTGCATCATCTGCTCAATTGAGACAATTGTTTGTCACAATAATTTTATTTTGTGATGTAGCAAATCCTCAAAATCTTTTTGATAGTCATTGGCAAAACATGTATGATGACATTTTATATAAATTGCGAAAATTTTTTGCAATTCCAGATATGATTGTGCCAGAACATGAATTAAAAAATTCAGTGCTATTTGAATTAGAATTACTTTTTAATAATGCATCTACCTCTTTAAAAAAATATCATCTTCCCATGCCTAATGCAGAAAAAATGTTAGAAATTCAAAATAGGCTTTTGAGAGAAGAATTAAATTATGATTGTGAAGAACTTTATAAGAAACATGCACTTTTGAAATCACAGCTTAACCAAGGTCAAAAACATGTTTATGATTGTGTTTTAAATGCTGTGAATGCAAAAAAAGGAGGTCTATTCTTTGTTTATGGTCATGGAGGCACTGGAAAAACATTTTTATGGGATACAATTACAAGTAAGCTTAGATCAGACGGTAAAATTGTTCTAGCTGTTGCATCATCAGGAATAGCATCATTATTATTACCAGGTGGTAGAACAGCCCATTCTAGATTTAAAATCCCTTTGATTATTACTGATTCTTCAATTTGTTCAATAAAGAAAAAAACTCATCTTGCAGGATTAATTGAAAAAACAGATTTAATTATATGGGATGAAGCACCTATGAATCATAAATATTGTTTTGAAGCATTGGATAAATCATTTTCAGATATTTTGTCAAATTCACAAGCTTTCGATAAAAATGTTCCATTTGGTGGAAAAACTCTTTTATTGGGTGGTGATTTTCGACAAATATTGCCTGTTATTCCTCAAGGAACTAAAGAGGATATAATAAATGCTTCATTGAATAGTTCTTATTTATGGCCATATTTTAAAATTTTTCATTTGAAAGAAAATATGAGATTGTCAAAAAATGGATTGAGTATTGAAGAAAAAAATAAAATTAGTTCTTTTGCAAATTGGATTCTACAAATTGGTGATGGACAAATTAATGATGCAAACAATGTGAATAATTCAATTGATAGGGATTCATCTTTGATTCAAATACCACAAGATTTGATAATTGATTGTTCTACAAATGCTATCCATTCAATTTTTTCTGCAACATACCCGAGTTTTGAAACTTTTTTTGACAATTTTGCTTATTTAAGGGAAAGAGCTATTATAACACCTCGAAATACAACTGTTACAGATGTGAATAATTATATAATTAATTTATTACCTGGACAACAACAAACATTTTTGAGTTATGATTCTTTATGTTCTTCAAATGCAAATGTTGAAAATCTTGAAATAATGTACCCCACAGAATTTTTAAACAAGCTTGAATTCAATGGTTTACCTGCACATAATTTGGTATTAAAAATAGGAATGCCTATTATGCTACTACGAAACTTAAATCAGTCTTCAGGTTTGTGTAATGGAACTAGATTAGTTGTTACTCAATTATTTGATAGAATTATTGAAGCTAAAATTCTTACTGGTACTAATATTGGTCATAAAGTTTTTATACCAAGAATCACTCTTACAGCTACAGAAAATAAGTGGCCTTTCATCTTCAAAAGACGTCAATTTCCAATTAGAGCATGTTATGCAATGACAATCAATAAAAGTCAAGGACAATCTCTTAAACAAGTTGGTTTATATCTTCCAGAACCTGTTTTTACACATGGCCAATTATATGTAGCATTATCAAGAGTGACATCAAGAGAAGGTTTAAAAATTTTAATAGAAAATGTAGAGAGTAGTGTTAAAAACTGTACTTTGAATATTGTTTTTAAAGATGTGTTACAAAATTTACAAGAAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460

Pfam Domains

Protein Families

Helitron_like_N Herpes_Helicase PIF1

Protein Analysis

818

Amino Acids

93.23

Weight (kDa)

8.86

Isoelectric Point (pI)

41.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PIF1	PF05970	324 - 548	1.3e-95	PIF1-like helicase
Pif1_2B_dom	PF21530	651 - 697	7.2e-23	DNA helicase Pif1, 2B domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001547)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51690 AT3G51700 AT5G28780
fragaria_vesca	FvH4_1g12810 FvH4_1g15091 FvH4_1g22681 FvH4_3g02850 FvH4_3g10331 FvH4_5g26611 FvH4_6g21911
malus_domestica	MD09G1257400.v1.1 MD10G1239600.v1.1 MD16G1146300.v1.1
prunus_persica	Prupe.1G200900_v2.0.a1 Prupe.1G234700_v2.0.a1 Prupe.6G165800_v2.0.a1
pyrus_communis	pycom01g15480 pycom03g19960 pycom05g09080 pycom05g09090 pycom05g09100 pycom05g13700 pycom05g27440 pycom06g13860 pycom09g02530 pycom10g28970 pycom11g03190 pycom11g03450 pycom11g03460 pycom15g06510 pycom15g06520 pycom15g09120 pycom15g25220 pycom15g25230 pycom17g19080
rosa_chinensis	RchiOBHm_Chr5g0080791
rosa_multiflora	Rmu_sc0002236.1_g000014 Rmu_sc0004733.1_g000008
rosa_roxburghii	Rroxscaffold_1G00025650 Rroxscaffold_3G00267580
rosa_rugosa	Rorug06G0008800
rosa_samantha	Rh1CG246000 Rh2BG332000 Rh2CG319700 Rh2DG369300 Rh3BG308100 Rh4CG020600 Rh6DG047800
rosa_wichuraiana	Rw4G009260 Rw5G045770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	8, 945, 1808
AatII	GACGTC	1 cut(s)	2212
Acc36I	ACCTGC	1 cut(s)	1993
AccB1I	GGYRCC	1 cut(s)	485
AccB7I	CCANNNNNTGG	1 cut(s)	1397
AcoI	YGGCCR	2 cut(s)	1505, 2314
AcuI	CTGAAG	1 cut(s)	2034
AcvI	CACGTG	1 cut(s)	323
AcyI	GRCGYC	2 cut(s)	486, 2209
AfaI	GTAC	5 cut(s)	429, 528, 1943, 2128, 2404
AfiI	CCNNNNNNNGG	2 cut(s)	440, 1397
AflIII	ACRYGT	2 cut(s)	714, 991
AjnI	CCWGG	2 cut(s)	1168, 1864
Alw21I	GWGCWC	1 cut(s)	52
Alw26I	GTCTC	2 cut(s)	130, 631
AlwNI	CAGNNNCTG	1 cut(s)	2300
Ama87I	CYCGRG	1 cut(s)	1753
AoxI	GGCC	3 cut(s)	1505, 2189, 2314
ApeKI	GCWGC	1 cut(s)	608
AseI	ATTAAT	5 cut(s)	252, 516, 1265, 1632, 1854
AspLEI	GCGC	1 cut(s)	488
AsuHPI	GGTGA	4 cut(s)	108, 584, 1433, 1631
AvaI	CYCGRG	1 cut(s)	1753
BalI	TGGCCA	2 cut(s)	1507, 2316
BanI	GGYRCC	1 cut(s)	485
BbrPI	CACGTG	1 cut(s)	323
BbsI	GAAGAC	1 cut(s)	2040
Bbv12I	GWGCWC	1 cut(s)	52
BbvI	GCAGC	1 cut(s)	595
BccI	CCATC	1 cut(s)	1616
BciT130I	CCWGG	2 cut(s)	1170, 1866
BciVI	GTATCC	1 cut(s)	1081
BclI	TGATCA	1 cut(s)	229
BcoDI	GTCTC	2 cut(s)	130, 631
BfaI	CTAG	3 cut(s)	1130, 1191, 2070
BfmI	CTRYAG	3 cut(s)	174, 239, 2175
BfoI	RGCGCY	1 cut(s)	489
BfuAI	ACCTGC	1 cut(s)	1993
BfuI	GTATCC	1 cut(s)	1081
BisI	GCNGC	1 cut(s)	609
BlsI	GCNGC	1 cut(s)	610
Bme1390I	CCNGG	2 cut(s)	1170, 1866
BmeT110I	CYCGRG	1 cut(s)	1753
BmiI	GGNNCC	1 cut(s)	487
BmrFI	CCNGG	2 cut(s)	1170, 1866
BmsI	GCATC	7 cut(s)	413, 446, 629, 842, 1148, 1163, 1627
BpiI	GAAGAC	1 cut(s)	2040
BpuEI	CTTGAG	1 cut(s)	1437
BsaAI	YACGTR	1 cut(s)	323
BsaBI	GATNNNNATC	1 cut(s)	112
BsaHI	GRCGYC	2 cut(s)	486, 2209
BsaJI	CCNNGG	1 cut(s)	979
Bsc4I	CCNNNNNNNGG	2 cut(s)	440, 1397
Bse1I	ACTGG	3 cut(s)	188, 1072, 2128
Bse3DI	GCAATG	1 cut(s)	2244
Bse8I	GATNNNNATC	1 cut(s)	112
BseBI	CCWGG	2 cut(s)	1170, 1866
BseDI	CCNNGG	1 cut(s)	979
BseGI	GGATG	3 cut(s)	430, 508, 1300
BseJI	GATNNNNATC	1 cut(s)	112
BseLI	CCNNNNNNNGG	2 cut(s)	440, 1397
BseMI	GCAATG	1 cut(s)	2244
BseNI	ACTGG	3 cut(s)	188, 1072, 2128
BseXI	GCAGC	1 cut(s)	595
BsgI	GTGCAG	2 cut(s)	594, 1971
BshFI	GGCC	3 cut(s)	1507, 2191, 2316
BshNI	GGYRCC	1 cut(s)	485
BsiHKAI	GWGCWC	1 cut(s)	52
BsiHKCI	CYCGRG	1 cut(s)	1753
BslI	CCNNNNNNNGG	2 cut(s)	440, 1397
BsmAI	GTCTC	2 cut(s)	130, 631
BsmI	GAATGC	3 cut(s)	468, 1027, 2019
BsnI	GGCC	3 cut(s)	1507, 2191, 2316
BsoBI	CYCGRG	1 cut(s)	1753
Bsp1286I	GDGCHC	1 cut(s)	52
Bsp1407I	TGTACA	2 cut(s)	427, 526
Bsp143I	GATC	2 cut(s)	229, 1109
BspANI	GGCC	3 cut(s)	1507, 2191, 2316
BspLI	GGNNCC	1 cut(s)	487
BspMI	ACCTGC	1 cut(s)	1993
BspT107I	GGYRCC	1 cut(s)	485
BsrDI	GCAATG	1 cut(s)	2244
BsrGI	TGTACA	2 cut(s)	427, 526
BsrI	ACTGG	3 cut(s)	188, 1072, 2128
BssECI	CCNNGG	1 cut(s)	979
BssMI	GATC	2 cut(s)	229, 1109
BssNI	GRCGYC	2 cut(s)	486, 2209
BssT1I	CCWWGG	1 cut(s)	979
Bst2UI	CCWGG	2 cut(s)	1170, 1866
Bst4CI	ACNGT	4 cut(s)	243, 1118, 1828, 2402
BstACI	GRCGYC	2 cut(s)	486, 2209
BstAPI	GCANNNNNTGC	3 cut(s)	617, 626, 2234
BstAUI	TGTACA	2 cut(s)	427, 526
BstBAI	YACGTR	1 cut(s)	323
BstC8I	GCNNGC	1 cut(s)	550
BstDEI	CTNAG	1 cut(s)	1106
BstF5I	GGATG	3 cut(s)	430, 508, 1300
BstH2I	RGCGCY	1 cut(s)	489
BstHHI	GCGC	1 cut(s)	488
BstKTI	GATC	2 cut(s)	232, 1112
BstMAI	GTCTC	2 cut(s)	130, 631
BstMBI	GATC	2 cut(s)	229, 1109
BstMWI	GCNNNNNNNGC	5 cut(s)	617, 626, 1022, 2023, 2234
BstNI	CCWGG	2 cut(s)	1170, 1866
BstNSI	RCATGY	5 cut(s)	552, 718, 956, 995, 2232
BstSCI	CCNGG	2 cut(s)	1168, 1864
BstSFI	CTRYAG	3 cut(s)	174, 239, 2175
BstV1I	GCAGC	1 cut(s)	595
BstV2I	GAAGAC	1 cut(s)	2040
BsuI	GTATCC	1 cut(s)	1081
BsuRI	GGCC	3 cut(s)	1507, 2191, 2316
BtsCI	GGATG	3 cut(s)	430, 508, 1300
BtsIMutI	CAGTG	2 cut(s)	804, 1065
BveI	ACCTGC	1 cut(s)	1993
Cac8I	GCNNGC	1 cut(s)	550
CaiI	CAGNNNCTG	1 cut(s)	2300
CfoI	GCGC	1 cut(s)	488
CsiI	ACCWGGT	1 cut(s)	1168
Csp6I	GTAC	5 cut(s)	428, 527, 1942, 2127, 2403
CspCI	CAANNNNNGTGG	2 cut(s)	1680, 1715
CviAII	CATG	9 cut(s)	549, 715, 782, 865, 953, 992, 1058, 2229, 2312
CviQI	GTAC	5 cut(s)	428, 527, 1942, 2127, 2403
DdeI	CTNAG	1 cut(s)	1106
DinI	GGCGCC	1 cut(s)	487
DpnI	GATC	2 cut(s)	231, 1111
DpnII	GATC	2 cut(s)	229, 1109
DraI	TTTAAA	8 cut(s)	477, 846, 1011, 1198, 1516, 1959, 2361, 2422
EaeI	YGGCCR	2 cut(s)	1505, 2314
Eco130I	CCWWGG	1 cut(s)	979
Eco57I	CTGAAG	1 cut(s)	2034
Eco72I	CACGTG	1 cut(s)	323
Eco88I	CYCGRG	1 cut(s)	1753
EcoRI	GAATTC	1 cut(s)	1970
EcoRII	CCWGG	2 cut(s)	1168, 1864
EcoT14I	CCWWGG	1 cut(s)	979
EcoT22I	ATGCAT	1 cut(s)	835
EgeI	GGCGCC	1 cut(s)	487
EheI	GGCGCC	1 cut(s)	487
ErhI	CCWWGG	1 cut(s)	979
FaeI	CATG	9 cut(s)	552, 718, 785, 868, 956, 995, 1061, 2232, 2315
FatI	CATG	9 cut(s)	548, 714, 781, 864, 952, 991, 1057, 2228, 2311
FauNDI	CATATG	1 cut(s)	424
FbaI	TGATCA	1 cut(s)	229
Fnu4HI	GCNGC	1 cut(s)	609
FokI	GGATG	3 cut(s)	417, 515, 1307
Fsp4HI	GCNGC	1 cut(s)	609
FspBI	CTAG	3 cut(s)	1130, 1191, 2070
GlaI	GCGC	1 cut(s)	487
GluI	GCNGC	1 cut(s)	609
HaeII	RGCGCY	1 cut(s)	489
HaeIII	GGCC	3 cut(s)	1507, 2191, 2316
HhaI	GCGC	1 cut(s)	488
Hin1I	GRCGYC	2 cut(s)	486, 2209
Hin1II	CATG	9 cut(s)	552, 718, 785, 868, 956, 995, 1061, 2232, 2315
Hin6I	GCGC	1 cut(s)	486
HinP1I	GCGC	1 cut(s)	486
HincII	GTYRAC	1 cut(s)	282
HindII	GTYRAC	1 cut(s)	282
HindIII	AAGCTT	4 cut(s)	261, 1103, 1371, 1964
HinfI	GANTC	7 cut(s)	1220, 1309, 1606, 1670, 1681, 1895, 2160
HpaI	GTTAAC	1 cut(s)	282
HphI	GGTGA	4 cut(s)	108, 584, 1433, 1631
Hpy166II	GTNNAC	3 cut(s)	282, 326, 1982
Hpy188I	TCNGA	3 cut(s)	43, 1114, 1351
Hpy188III	TCNNGA	8 cut(s)	65, 761, 1146, 1191, 1931, 2294, 2337, 2349
Hpy8I	GTNNAC	3 cut(s)	282, 326, 1982
HpyAV	CCTTC	2 cut(s)	180, 2348
HpyCH4III	ACNGT	4 cut(s)	243, 1118, 1828, 2402
HpyCH4IV	ACGT	3 cut(s)	52, 322, 2209
HpyF10VI	GCNNNNNNNGC	5 cut(s)	617, 626, 1022, 2023, 2234
HpyF3I	CTNAG	1 cut(s)	1106
HpySE526I	ACGT	3 cut(s)	52, 322, 2209
Hsp92I	GRCGYC	2 cut(s)	486, 2209
Hsp92II	CATG	9 cut(s)	552, 718, 785, 868, 956, 995, 1061, 2232, 2315
HspAI	GCGC	1 cut(s)	486
KasI	GGCGCC	1 cut(s)	485
Ksp22I	TGATCA	1 cut(s)	229
KspAI	GTTAAC	1 cut(s)	282
Kzo9I	GATC	2 cut(s)	229, 1109
Lsp1109I	GCAGC	1 cut(s)	595
LweI	GCATC	7 cut(s)	413, 446, 629, 842, 1148, 1163, 1627
MabI	ACCWGGT	1 cut(s)	1168
MaeI	CTAG	3 cut(s)	1130, 1191, 2070
MaeII	ACGT	3 cut(s)	52, 322, 2209
MaeIII	GTNAC	5 cut(s)	649, 1828, 2080, 2341, 2430
MalI	GATC	2 cut(s)	231, 1111
MboI	GATC	2 cut(s)	229, 1109
MfeI	CAATTG	5 cut(s)	100, 408, 632, 641, 1659
MhlI	GDGCHC	1 cut(s)	52
MlsI	TGGCCA	2 cut(s)	1507, 2316
MluNI	TGGCCA	2 cut(s)	1507, 2316
Mly113I	GGCGCC	1 cut(s)	486
Mox20I	TGGCCA	2 cut(s)	1507, 2316
Mph1103I	ATGCAT	1 cut(s)	835
MscI	TGGCCA	2 cut(s)	1507, 2316
MslI	CAYNNNNRTG	2 cut(s)	996, 1081
Msp20I	TGGCCA	2 cut(s)	1507, 2316
MspA1I	CMGCKG	1 cut(s)	227
MspR9I	CCNGG	2 cut(s)	1170, 1866
MunI	CAATTG	5 cut(s)	100, 408, 632, 641, 1659
Mva1269I	GAATGC	3 cut(s)	468, 1027, 2019
MvaI	CCWGG	2 cut(s)	1170, 1866
MwoI	GCNNNNNNNGC	5 cut(s)	617, 626, 1022, 2023, 2234
NarI	GGCGCC	1 cut(s)	486
NdeI	CATATG	1 cut(s)	424
NdeII	GATC	2 cut(s)	229, 1109
NlaIII	CATG	9 cut(s)	552, 718, 785, 868, 956, 995, 1061, 2232, 2315
NlaIV	GGNNCC	1 cut(s)	487
NmuCI	GTSAC	2 cut(s)	649, 2341
NsiI	ATGCAT	1 cut(s)	835
NspI	RCATGY	5 cut(s)	552, 718, 956, 995, 2232
PaeI	GCATGC	1 cut(s)	552
PciI	ACATGT	2 cut(s)	714, 991
PctI	GAATGC	3 cut(s)	468, 1027, 2019
PfeI	GAWTC	7 cut(s)	1220, 1309, 1606, 1670, 1681, 1895, 2160
PflMI	CCANNNNNTGG	1 cut(s)	1397
PkrI	GCNGC	1 cut(s)	610
PluTI	GGCGCC	1 cut(s)	489
PmaCI	CACGTG	1 cut(s)	323
PmlI	CACGTG	1 cut(s)	323
Ppu21I	YACGTR	1 cut(s)	323
PscI	ACATGT	2 cut(s)	714, 991
PshBI	ATTAAT	5 cut(s)	252, 516, 1265, 1632, 1854
PsiI	TTATAA	3 cut(s)	8, 945, 1808
Psp6I	CCWGG	2 cut(s)	1168, 1864
PspCI	CACGTG	1 cut(s)	323
PspGI	CCWGG	2 cut(s)	1168, 1864
PspN4I	GGNNCC	1 cut(s)	487
PstNI	CAGNNNCTG	1 cut(s)	2300
PvuII	CAGCTG	1 cut(s)	227
RsaI	GTAC	5 cut(s)	429, 528, 1943, 2128, 2404
RsaNI	GTAC	5 cut(s)	428, 527, 1942, 2127, 2403
RseI	CAYNNNNRTG	2 cut(s)	996, 1081
SatI	GCNGC	1 cut(s)	609
Sau3AI	GATC	2 cut(s)	229, 1109
ScrFI	CCNGG	2 cut(s)	1170, 1866
SduI	GDGCHC	1 cut(s)	52
SexAI	ACCWGGT	1 cut(s)	1168
SfaNI	GCATC	7 cut(s)	413, 446, 629, 842, 1148, 1163, 1627
SfcI	CTRYAG	3 cut(s)	174, 239, 2175
SfoI	GGCGCC	1 cut(s)	487
SmiMI	CAYNNNNRTG	2 cut(s)	996, 1081
SmlI	CTYRAG	1 cut(s)	1452
SmoI	CTYRAG	1 cut(s)	1452
SphI	GCATGC	1 cut(s)	552
SspDI	GGCGCC	1 cut(s)	485
SspI	AATATT	6 cut(s)	14, 149, 1319, 1437, 2134, 2413
SspMI	CTAG	3 cut(s)	1130, 1191, 2070
StyD4I	CCNGG	2 cut(s)	1168, 1864
StyI	CCWWGG	1 cut(s)	979
TaaI	ACNGT	4 cut(s)	243, 1118, 1828, 2402
TaiI	ACGT	3 cut(s)	55, 325, 2212
TaqI	TCGA	3 cut(s)	1377, 1429, 1816
TatI	WGTACW	3 cut(s)	427, 526, 2402
TfiI	GAWTC	7 cut(s)	1220, 1309, 1606, 1670, 1681, 1895, 2160
TscAI	CASTG	2 cut(s)	804, 1072
TseFI	GTSAC	2 cut(s)	649, 2341
TseI	GCWGC	1 cut(s)	608
Tsp45I	GTSAC	2 cut(s)	649, 2341
TspDTI	ATGAA	9 cut(s)	142, 182, 798, 1311, 1322, 1473, 1514, 1662, 2185
TspRI	CASTG	2 cut(s)	804, 1072
Van91I	CCANNNNNTGG	1 cut(s)	1397
VspI	ATTAAT	5 cut(s)	252, 516, 1265, 1632, 1854
XbaI	TCTAGA	1 cut(s)	1190
XceI	RCATGY	5 cut(s)	552, 718, 956, 995, 2232
XspI	CTAG	3 cut(s)	1130, 1191, 2070
ZraI	GACGTC	1 cut(s)	2210
Zsp2I	ATGCAT	1 cut(s)	835

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.