pycom15g06510

G-quadruplex DNA unwinding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001547
Physical Location & Seq
Forward (+)
3994706 .. 3996274
1569 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g06510.1

Sequence Viewer

Length: 1494 bp
ATGTTTGCATTCACTTCAATGGGAGCCAAAGTTGATAACTCAATTAACAATGGATCAAGCCCTTATATTTTCAAAATCAGTGGTCAAGTTTGTCATTTAATGGGATCTCTTTTGCCTGTTGATAATGAGTCTCCAAAATTTGCTCAACTGTATGTCTATGATACATATAATGAGGTGGAAAATCGACTTAAAGTTTTTAATTCCAATGGAAACAATCAAAGCCTAGATCCAACAATTGTAGAAGGGTTAATCAAAATGTTTGATGCATCAAATGAATTGGTTAAACTTTTTCGAATGGCAAGAGATAAATTTGAAAACGATGCTCTTTCTTCATTAAAAATGACTTTGCTAGGCAGGCAATTAAATGATAGCAAACAATATGAGCAACCTACAACTGATGAAATAGGTGGTTTGATAGTTGGAGATATAGGATTGTATGATTCTAATCGAGATATTATTATAGAGTCTAAAAGTCACGAATTAAAACGTGTAACAAAGTTGAATCCTAAATTTATGTCTCTGCAATATCCAATTCTTTTTCCATATGGTGAAGATGGTTATAGACCAGATTTAAAATGGAATGAAAATTATAAAGGTAAAAAAACTAAAAGACAAAGAATACCAATGAGAGCTTTCATTGCTTATCAAATTCAAGAACGAGAACCACCATTAAGTACATTGTTAAGAGGAGGAAGACTTTTTCAACAATATTTGGTCGATTCTTTTGCAACACTTGAAGAAGATAGATTAGATTATATTAGACAAAATCAAAAAAATTTGAGAAGTGAAGTTTATAAAGGAATATATGATGCATTCTCAAAAGGTGATACTAATGCAAATAATTTGGGACAAAAAATTGTATTACCTGCTTCATATACTGGAAGTCCTAGATATGTGATAAATAATTATCAAGATGCTATGGCTATTTGTAGAGAATACGGACATCCTGATTTGTTTATAACATTTACATGTAATGTTAAATGGTCAGAAATCACAAGAGAGTTTGAGAATAAGCCAGGATTCAAACCTGAAGATAGACCTGATATAATTTCTAGAATCTTCAAAATGAAGCTTGATCATATGATTGATTTCATCAAGTCCGGGAAGCCTTTTGGAGAAATTCAAGCAAGTAAGTTACTAAAACTCATTCTAGCACTTATTTCAAATGTTTGCACAGTTGAGTTTCAAAAAAGAGGTTTACCTCATTCTCATATGTTAATATGGCTGAAACCAAATGTAAAATGTTTTTGCGCATCAGATATAGACTCAATTATTTCTGCTGAATTACCTGATAAAAATTTGCATCCACAACTTTATGAAACTGTTAATCAGTTTATGATTCATGGTCCATGTGGTTGTATTAATCCAAAATCACCTTGCATGCGAGAAAATAAATGTTCTAAAAACTTTCCTAAGCCATATAAAAGCGAAACAATTTTGACACAAATGGTTTTGCTGTTTATAAACGTCGTGAAGATGAAGAAAAATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

498

Amino Acids

57.1

Weight (kDa)

8.89

Isoelectric Point (pI)

31.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 212 - 407 1.3e-51 Helitron helicase-like domain at N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 591, 795, 959, 1463
Acc16I TGCGCA 1 cut(s) 1252
Acc36I ACCTGC 1 cut(s) 874
AclWI GGATC 3 cut(s) 61, 112, 221
AcsI RAATTY 8 cut(s) 137, 308, 509, 648, 775, 1119, 1297, 1486
AcuI CTGAAG 1 cut(s) 1050
AfaI GTAC 1 cut(s) 676
AflIII ACRYGT 2 cut(s) 487, 968
AjnI CCWGG 1 cut(s) 1015
AluBI AGCT 2 cut(s) 632, 1072
AluI AGCT 2 cut(s) 632, 1072
Alw26I GTCTC 2 cut(s) 135, 522
AlwI GGATC 3 cut(s) 61, 112, 221
ApoI RAATTY 8 cut(s) 137, 308, 509, 648, 775, 1119, 1297, 1486
AseI ATTAAT 1 cut(s) 1362
AspLEI GCGC 1 cut(s) 1253
AspS9I GGNCC 1 cut(s) 1346
AsuC2I CCSGG 1 cut(s) 1102
AsuHPI GGTGA 3 cut(s) 560, 836, 1365
AsuII TTCGAA 1 cut(s) 292
AvaII GGWCC 1 cut(s) 1346
BbsI GAAGAC 1 cut(s) 700
BccI CCATC 1 cut(s) 548
BcgI CGANNNNNNTGC 2 cut(s) 707, 741
BciT130I CCWGG 1 cut(s) 1017
BclI TGATCA 1 cut(s) 1075
BcnI CCSGG 1 cut(s) 1102
BcoDI GTCTC 2 cut(s) 135, 522
BfaI CTAG 5 cut(s) 224, 350, 888, 1053, 1151
BfuAI ACCTGC 1 cut(s) 874
Bme1390I CCNGG 2 cut(s) 1017, 1102
Bme18I GGWCC 1 cut(s) 1346
BmgT120I GGNCC 1 cut(s) 1346
BmiI GGNNCC 1 cut(s) 25
BmrFI CCNGG 2 cut(s) 1017, 1102
BmsI GCATC 7 cut(s) 253, 275, 310, 799, 904, 1262, 1312
BpiI GAAGAC 1 cut(s) 700
Bpu10I CCTNAGC 1 cut(s) 1413
Bpu14I TTCGAA 1 cut(s) 292
BpuMI CCSGG 1 cut(s) 1102
BsaBI GATNNNNATC 1 cut(s) 444
BsaXI ACNNNNNCTCC 2 cut(s) 15, 45
Bse1I ACTGG 1 cut(s) 883
Bse3DI GCAATG 1 cut(s) 636
Bse8I GATNNNNATC 1 cut(s) 444
BseBI CCWGG 1 cut(s) 1017
BseGI GGATG 2 cut(s) 943, 1303
BseJI GATNNNNATC 1 cut(s) 444
BseMI GCAATG 1 cut(s) 636
BseNI ACTGG 1 cut(s) 883
BseRI GAGGAG 1 cut(s) 702
BsiSI CCGG 1 cut(s) 1101
BslFI GGGAC 1 cut(s) 861
BsmAI GTCTC 2 cut(s) 135, 522
BsmFI GGGAC 1 cut(s) 861
BsmI GAATGC 2 cut(s) 8, 812
Bsp119I TTCGAA 1 cut(s) 292
Bsp143I GATC 4 cut(s) 53, 104, 226, 1075
BspLI GGNNCC 1 cut(s) 25
BspMI ACCTGC 1 cut(s) 874
BspPI GGATC 3 cut(s) 61, 112, 221
BspT104I TTCGAA 1 cut(s) 292
BsrDI GCAATG 1 cut(s) 636
BsrI ACTGG 1 cut(s) 883
BssMI GATC 4 cut(s) 53, 104, 226, 1075
Bst2UI CCWGG 1 cut(s) 1017
Bst4CI ACNGT 3 cut(s) 150, 1177, 1324
BstBI TTCGAA 1 cut(s) 292
BstC8I GCNNGC 2 cut(s) 356, 1382
BstDEI CTNAG 1 cut(s) 1413
BstF5I GGATG 2 cut(s) 943, 1303
BstHHI GCGC 1 cut(s) 1253
BstKTI GATC 4 cut(s) 56, 107, 229, 1078
BstMAI GTCTC 2 cut(s) 135, 522
BstMBI GATC 4 cut(s) 53, 104, 226, 1075
BstMWI GCNNNNNNNGC 2 cut(s) 355, 638
BstNI CCWGG 1 cut(s) 1017
BstNSI RCATGY 2 cut(s) 972, 1384
BstSCI CCNGG 2 cut(s) 1015, 1100
BstV2I GAAGAC 1 cut(s) 700
BstX2I RGATCY 2 cut(s) 104, 226
BstYI RGATCY 2 cut(s) 104, 226
BtsCI GGATG 2 cut(s) 943, 1303
BtsIMutI CAGTG 1 cut(s) 85
BveI ACCTGC 1 cut(s) 874
Cac8I GCNNGC 2 cut(s) 356, 1382
CfoI GCGC 1 cut(s) 1253
Cfr13I GGNCC 1 cut(s) 1346
Csp6I GTAC 1 cut(s) 675
CspCI CAANNNNNGTGG 2 cut(s) 61, 96
CviAII CATG 4 cut(s) 969, 1343, 1350, 1381
CviQI GTAC 1 cut(s) 675
DdeI CTNAG 1 cut(s) 1413
DpnI GATC 4 cut(s) 55, 106, 228, 1077
DpnII GATC 4 cut(s) 53, 104, 226, 1075
DraI TTTAAA 1 cut(s) 573
Eco47I GGWCC 1 cut(s) 1346
Eco57I CTGAAG 1 cut(s) 1050
EcoRII CCWGG 1 cut(s) 1015
EcoT22I ATGCAT 2 cut(s) 268, 814
FaeI CATG 4 cut(s) 972, 1346, 1353, 1384
FaqI GGGAC 1 cut(s) 861
FatI CATG 4 cut(s) 968, 1342, 1349, 1380
FauNDI CATATG 3 cut(s) 544, 1080, 1212
FbaI TGATCA 1 cut(s) 1075
FokI GGATG 2 cut(s) 930, 1290
FspBI CTAG 5 cut(s) 224, 350, 888, 1053, 1151
FspI TGCGCA 1 cut(s) 1252
GlaI GCGC 1 cut(s) 1252
HapII CCGG 1 cut(s) 1101
HhaI GCGC 1 cut(s) 1253
Hin1II CATG 4 cut(s) 972, 1346, 1353, 1384
Hin6I GCGC 1 cut(s) 1251
HinP1I GCGC 1 cut(s) 1251
HindIII AAGCTT 1 cut(s) 1070
HinfI GANTC 9 cut(s) 128, 440, 464, 502, 719, 1020, 1056, 1265, 1339
HpaII CCGG 1 cut(s) 1101
HphI GGTGA 3 cut(s) 560, 836, 1365
Hpy166II GTNNAC 1 cut(s) 1199
Hpy188I TCNGA 2 cut(s) 988, 1258
Hpy188III TCNNGA 7 cut(s) 449, 476, 653, 911, 947, 1053, 1471
Hpy8I GTNNAC 1 cut(s) 1199
Hpy99I CGWCG 1 cut(s) 1472
HpyAV CCTTC 1 cut(s) 236
HpyCH4III ACNGT 3 cut(s) 150, 1177, 1324
HpyCH4IV ACGT 2 cut(s) 487, 1467
HpyCH4V TGCA 9 cut(s) 8, 266, 523, 728, 812, 836, 1173, 1303, 1380
HpyF10VI GCNNNNNNNGC 2 cut(s) 355, 638
HpyF3I CTNAG 1 cut(s) 1413
HpySE526I ACGT 2 cut(s) 487, 1467
Hsp92II CATG 4 cut(s) 972, 1346, 1353, 1384
HspAI GCGC 1 cut(s) 1251
Ksp22I TGATCA 1 cut(s) 1075
Kzo9I GATC 4 cut(s) 53, 104, 226, 1075
LmnI GCTCC 1 cut(s) 23
LweI GCATC 7 cut(s) 253, 275, 310, 799, 904, 1262, 1312
MaeI CTAG 5 cut(s) 224, 350, 888, 1053, 1151
MaeII ACGT 2 cut(s) 487, 1467
MaeIII GTNAC 3 cut(s) 473, 490, 1134
MalI GATC 4 cut(s) 55, 106, 228, 1077
MboI GATC 4 cut(s) 53, 104, 226, 1075
MboII GAAGA 9 cut(s) 321, 563, 705, 749, 752, 1043, 1051, 1486, 1492
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 2 cut(s) 104, 226
MlyI GAGTC 3 cut(s) 137, 473, 1259
MmeI TCCRAC 2 cut(s) 254, 400
MnlI CCTC 5 cut(s) 166, 680, 683, 1187, 1212
Mph1103I ATGCAT 2 cut(s) 268, 814
MslI CAYNNNNRTG 2 cut(s) 17, 967
MspI CCGG 1 cut(s) 1101
MspR9I CCNGG 2 cut(s) 1017, 1102
MunI CAATTG 1 cut(s) 234
Mva1269I GAATGC 2 cut(s) 8, 812
MvaI CCWGG 1 cut(s) 1017
MwoI GCNNNNNNNGC 2 cut(s) 355, 638
NciI CCSGG 1 cut(s) 1102
NdeI CATATG 3 cut(s) 544, 1080, 1212
NdeII GATC 4 cut(s) 53, 104, 226, 1075
NlaIII CATG 4 cut(s) 972, 1346, 1353, 1384
NlaIV GGNNCC 1 cut(s) 25
NmuCI GTSAC 1 cut(s) 473
NsbI TGCGCA 1 cut(s) 1252
NsiI ATGCAT 2 cut(s) 268, 814
NspI RCATGY 2 cut(s) 972, 1384
NspV TTCGAA 1 cut(s) 292
PaeI GCATGC 1 cut(s) 1384
PciI ACATGT 1 cut(s) 968
PctI GAATGC 2 cut(s) 8, 812
PfeI GAWTC 6 cut(s) 440, 502, 719, 1020, 1056, 1339
PfoI TCCNGGA 1 cut(s) 1100
PleI GAGTC 3 cut(s) 136, 472, 1259
PpsI GAGTC 3 cut(s) 136, 472, 1259
PscI ACATGT 1 cut(s) 968
PshBI ATTAAT 1 cut(s) 1362
PsiI TTATAA 4 cut(s) 591, 795, 959, 1463
Psp6I CCWGG 1 cut(s) 1015
PspGI CCWGG 1 cut(s) 1015
PspN4I GGNNCC 1 cut(s) 25
PspPI GGNCC 1 cut(s) 1346
PsuI RGATCY 2 cut(s) 104, 226
RsaI GTAC 1 cut(s) 676
RsaNI GTAC 1 cut(s) 675
RseI CAYNNNNRTG 2 cut(s) 17, 967
Sau3AI GATC 4 cut(s) 53, 104, 226, 1075
Sau96I GGNCC 1 cut(s) 1346
SchI GAGTC 3 cut(s) 137, 473, 1259
ScrFI CCNGG 2 cut(s) 1017, 1102
SfaNI GCATC 7 cut(s) 253, 275, 310, 799, 904, 1262, 1312
SfuI TTCGAA 1 cut(s) 292
SinI GGWCC 1 cut(s) 1346
SmiMI CAYNNNNRTG 2 cut(s) 17, 967
SphI GCATGC 1 cut(s) 1384
SspI AATATT 1 cut(s) 710
SspMI CTAG 5 cut(s) 224, 350, 888, 1053, 1151
StyD4I CCNGG 2 cut(s) 1015, 1100
TaaI ACNGT 3 cut(s) 150, 1177, 1324
TaiI ACGT 2 cut(s) 490, 1470
TaqI TCGA 4 cut(s) 184, 292, 448, 717
TatI WGTACW 1 cut(s) 674
TfiI GAWTC 6 cut(s) 440, 502, 719, 1020, 1056, 1339
TscAI CASTG 1 cut(s) 85
TseFI GTSAC 1 cut(s) 473
Tsp45I GTSAC 1 cut(s) 473
TspGWI ACGGA 1 cut(s) 954
TspRI CASTG 1 cut(s) 85
VpaK11BI GGWCC 1 cut(s) 1346
VspI ATTAAT 1 cut(s) 1362
XapI RAATTY 8 cut(s) 137, 308, 509, 648, 775, 1119, 1297, 1486
XbaI TCTAGA 1 cut(s) 1052
XceI RCATGY 2 cut(s) 972, 1384
XcmI CCANNNNNNNNNTGG 1 cut(s) 573
XspI CTAG 5 cut(s) 224, 350, 888, 1053, 1151
Zsp2I ATGCAT 2 cut(s) 268, 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.