MD10G1239600.v1.1

G-quadruplex DNA unwinding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001547
Physical Location & Seq
Forward (+)
33538168 .. 33544315
6148 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1239600.v1.1.491

Sequence Viewer

Length: 1182 bp
ATGTTTTCATTTACTTCGATGGGAGCCAAAATTGATCACTCAATTAATGATGGTTCAGGTCCTTATATTTTCAAAATTAGTGGTCAAGTTTGCCATTTGATGGGATCTCTGTTGCCTGCTGACAATGAGTGTCCAAAATTTGCACAATTATATGTCTATGATACATACAATGAAGTGCAAAATCGATTGAAGGCTTTTGAATCTGATGGAAATAATAGAAAGCTTGATCCAAAAATTGTTGAAGGTTTGATTAAGATGTTTGATATGTCAAATGAGTTAGTTAAGCTTTTTCGAACAGCAAGAGATAAGTTTGAAACTAATAGACTCACTTCTTTGAAAATGAGATTATCAGCAAGAGAAACAACAGATGGTAAACAATATGACCAACCAACAAGTGATGAAATAAGTGGTCTGATAGTTGGTGATATAGGACTTGCTGATTCTAATAGAGATATTATTATTGAGTCTAAAAGTGAAGGATTAAAGCGTGTTACAAAATTAAATCCAAAATTTATGTCTTTGCAATATCCTCTTCTTTTCCCATACGGTGAAGATGGTTATAGACCTGATTTAAAATGGAACAAAAATTATAAGGGTTCAGAGACTAAAAGACAAAGGATACCCATGAGAGCTTTTATAGCGTATCAAATTCAAGAACGAGAACCATGCTTAACAACATTGTTAAAAGGGGGGAGACTTTTTCAACAATATTTGGTTGATTCTTATGCAACACTTGAAGAAGATAGGTTAGATTACATTAGGCAAAACCAAAAAAATTTAAGAAGTGAAGTTTACAAAGGAATTTATGATGCGATGTCAAGGGGTGATAATGATGCAAACAATTTGGGACAAAAAATTGTATTGCCTGCTTCATACACAGGGAGTCCTAGATATATGATTAATAATTATCAAGATGCAATGGCTATTTGCAGAGAATATGGACATCCTGATTTATTCATAACATTCACATGCAATATAAAGTGGCCAGAAATCATAAGAGAATTTCAGAAAAAACCAGGATTCAAACCTGAAGATAGGCCTGATATAATTTCCAGAGTATTTAAAATGAAACTCGATGATATGCTTCACTTTATAAAATCTGGAAAACCTTTTGGAGAAGTTGAAGCAAATGTTTGCACGATTGAATTTCAAAAAAGAGATGCCATCCAAGTTTTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

394

Amino Acids

45.4

Weight (kDa)

8.51

Isoelectric Point (pI)

35.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 212 - 387 2.4e-50 Helitron helicase-like domain at N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 591, 1094
AccB7I CCANNNNNTGG 1 cut(s) 100
AclWI GGATC 2 cut(s) 112, 221
AcoI YGGCCR 1 cut(s) 983
AcsI RAATTY 7 cut(s) 137, 509, 648, 775, 801, 1001, 1145
AcuI CTGAAG 1 cut(s) 1050
AfiI CCNNNNNNNGG 1 cut(s) 100
AjnI CCWGG 1 cut(s) 1015
AluBI AGCT 3 cut(s) 223, 286, 632
AluI AGCT 3 cut(s) 223, 286, 632
Alw26I GTCTC 2 cut(s) 596, 688
AlwI GGATC 2 cut(s) 112, 221
AoxI GGCC 2 cut(s) 983, 1037
ApoI RAATTY 7 cut(s) 137, 509, 648, 775, 801, 1001, 1145
ArsI GACNNNNNNTTYG 2 cut(s) 500, 532
AseI ATTAAT 2 cut(s) 45, 900
AspS9I GGNCC 1 cut(s) 59
AsuHPI GGTGA 3 cut(s) 434, 560, 836
AsuII TTCGAA 1 cut(s) 292
AvaII GGWCC 1 cut(s) 59
BalI TGGCCA 1 cut(s) 985
BccI CCATC 7 cut(s) 13, 44, 94, 200, 362, 548, 1172
BcgI CGANNNNNNTGC 2 cut(s) 648, 682
BciT130I CCWGG 1 cut(s) 1017
BciVI GTATCC 1 cut(s) 612
BclI TGATCA 1 cut(s) 34
BcoDI GTCTC 2 cut(s) 596, 688
BfaI CTAG 1 cut(s) 888
BfuI GTATCC 1 cut(s) 612
Bme1390I CCNGG 1 cut(s) 1017
Bme18I GGWCC 1 cut(s) 59
BmgT120I GGNCC 1 cut(s) 59
BmiI GGNNCC 1 cut(s) 25
BmrFI CCNGG 1 cut(s) 1017
BmsI GCATC 4 cut(s) 799, 823, 904, 1150
Bpu14I TTCGAA 1 cut(s) 292
Bsa29I ATCGAT 1 cut(s) 184
Bsc4I CCNNNNNNNGG 1 cut(s) 100
Bse3DI GCAATG 1 cut(s) 924
BseBI CCWGG 1 cut(s) 1017
BseCI ATCGAT 1 cut(s) 184
BseGI GGATG 2 cut(s) 943, 1164
BseLI CCNNNNNNNGG 1 cut(s) 100
BseMI GCAATG 1 cut(s) 924
BshFI GGCC 2 cut(s) 985, 1039
BshVI ATCGAT 1 cut(s) 184
BslFI GGGAC 1 cut(s) 861
BslI CCNNNNNNNGG 1 cut(s) 100
BsmAI GTCTC 2 cut(s) 596, 688
BsmFI GGGAC 1 cut(s) 861
BsnI GGCC 2 cut(s) 985, 1039
Bsp119I TTCGAA 1 cut(s) 292
Bsp143I GATC 3 cut(s) 34, 104, 226
BspANI GGCC 2 cut(s) 985, 1039
BspDI ATCGAT 1 cut(s) 184
BspLI GGNNCC 1 cut(s) 25
BspPI GGATC 2 cut(s) 112, 221
BspT104I TTCGAA 1 cut(s) 292
BsrDI GCAATG 1 cut(s) 924
BssMI GATC 3 cut(s) 34, 104, 226
Bst2UI CCWGG 1 cut(s) 1017
Bst4CI ACNGT 1 cut(s) 548
Bst6I CTCTTC 1 cut(s) 537
BstBI TTCGAA 1 cut(s) 292
BstC8I GCNNGC 2 cut(s) 117, 867
BstF5I GGATG 2 cut(s) 943, 1164
BstKTI GATC 3 cut(s) 37, 107, 229
BstMAI GTCTC 2 cut(s) 596, 688
BstMBI GATC 3 cut(s) 34, 104, 226
BstMWI GCNNNNNNNGC 1 cut(s) 638
BstNI CCWGG 1 cut(s) 1017
BstNSI RCATGY 1 cut(s) 972
BstSCI CCNGG 1 cut(s) 1015
BstX2I RGATCY 1 cut(s) 104
BstYI RGATCY 1 cut(s) 104
Bsu15I ATCGAT 1 cut(s) 184
BsuI GTATCC 1 cut(s) 612
BsuRI GGCC 2 cut(s) 985, 1039
BsuTUI ATCGAT 1 cut(s) 184
BtgZI GCGATG 1 cut(s) 827
BtsCI GGATG 2 cut(s) 943, 1164
Cac8I GCNNGC 2 cut(s) 117, 867
Cfr13I GGNCC 1 cut(s) 59
ClaI ATCGAT 1 cut(s) 184
CspCI CAANNNNNGTGG 2 cut(s) 61, 96
CviAII CATG 3 cut(s) 625, 666, 969
CviJI RGCY 8 cut(s) 26, 194, 223, 286, 632, 923, 985, 1039
CviKI_1 RGCY 8 cut(s) 26, 194, 223, 286, 632, 923, 985, 1039
DpnI GATC 3 cut(s) 36, 106, 228
DpnII GATC 3 cut(s) 34, 104, 226
DraI TTTAAA 2 cut(s) 573, 1063
EaeI YGGCCR 1 cut(s) 983
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
Eco147I AGGCCT 1 cut(s) 1039
Eco47I GGWCC 1 cut(s) 59
Eco57I CTGAAG 1 cut(s) 1050
EcoO109I RGGNCCY 1 cut(s) 59
EcoRII CCWGG 1 cut(s) 1015
FaeI CATG 3 cut(s) 628, 669, 972
FaqI GGGAC 1 cut(s) 861
FatI CATG 3 cut(s) 624, 665, 968
FbaI TGATCA 1 cut(s) 34
FokI GGATG 2 cut(s) 930, 1151
FspBI CTAG 1 cut(s) 888
HaeIII GGCC 2 cut(s) 985, 1039
Hin1II CATG 3 cut(s) 628, 669, 972
HindIII AAGCTT 2 cut(s) 221, 284
HinfI GANTC 7 cut(s) 200, 324, 440, 464, 719, 883, 1020
HphI GGTGA 3 cut(s) 434, 560, 836
Hpy166II GTNNAC 2 cut(s) 374, 793
Hpy188I TCNGA 4 cut(s) 205, 414, 601, 1008
Hpy188III TCNNGA 5 cut(s) 653, 911, 947, 1053, 1101
Hpy8I GTNNAC 2 cut(s) 374, 793
HpyAV CCTTC 3 cut(s) 184, 236, 470
HpyCH4III ACNGT 1 cut(s) 548
HpyCH4V TGCA 9 cut(s) 143, 178, 523, 728, 836, 917, 930, 972, 1137
HpyF10VI GCNNNNNNNGC 1 cut(s) 638
Hsp92II CATG 3 cut(s) 628, 669, 972
Ksp22I TGATCA 1 cut(s) 34
Kzo9I GATC 3 cut(s) 34, 104, 226
LmnI GCTCC 1 cut(s) 23
LweI GCATC 4 cut(s) 799, 823, 904, 1150
MaeI CTAG 1 cut(s) 888
MaeIII GTNAC 1 cut(s) 490
MalI GATC 3 cut(s) 36, 106, 228
MboI GATC 3 cut(s) 34, 104, 226
MboII GAAGA 5 cut(s) 524, 563, 749, 752, 1043
MflI RGATCY 1 cut(s) 104
MlsI TGGCCA 1 cut(s) 985
MluNI TGGCCA 1 cut(s) 985
MlyI GAGTC 3 cut(s) 318, 473, 892
MnlI CCTC 1 cut(s) 540
Mox20I TGGCCA 1 cut(s) 985
MscI TGGCCA 1 cut(s) 985
MslI CAYNNNNRTG 1 cut(s) 967
Msp20I TGGCCA 1 cut(s) 985
MspR9I CCNGG 1 cut(s) 1017
MvaI CCWGG 1 cut(s) 1017
MwoI GCNNNNNNNGC 1 cut(s) 638
NdeII GATC 3 cut(s) 34, 104, 226
NlaIII CATG 3 cut(s) 628, 669, 972
NlaIV GGNNCC 1 cut(s) 25
NspI RCATGY 1 cut(s) 972
NspV TTCGAA 1 cut(s) 292
PceI AGGCCT 1 cut(s) 1039
PfeI GAWTC 4 cut(s) 200, 440, 719, 1020
PflMI CCANNNNNTGG 1 cut(s) 100
PleI GAGTC 3 cut(s) 318, 472, 891
PpsI GAGTC 3 cut(s) 318, 472, 891
PpuMI RGGWCCY 1 cut(s) 59
PshBI ATTAAT 2 cut(s) 45, 900
PsiI TTATAA 2 cut(s) 591, 1094
Psp5II RGGWCCY 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 1015
PspGI CCWGG 1 cut(s) 1015
PspN4I GGNNCC 1 cut(s) 25
PspPI GGNCC 1 cut(s) 59
PspPPI RGGWCCY 1 cut(s) 59
PsuI RGATCY 1 cut(s) 104
RseI CAYNNNNRTG 1 cut(s) 967
Sau3AI GATC 3 cut(s) 34, 104, 226
Sau96I GGNCC 1 cut(s) 59
SchI GAGTC 3 cut(s) 318, 473, 892
ScrFI CCNGG 1 cut(s) 1017
SetI ASST 9 cut(s) 61, 225, 247, 288, 568, 634, 749, 1030, 1111
SfaNI GCATC 4 cut(s) 799, 823, 904, 1150
SfuI TTCGAA 1 cut(s) 292
SinI GGWCC 1 cut(s) 59
SmiMI CAYNNNNRTG 1 cut(s) 967
SseBI AGGCCT 1 cut(s) 1039
SspI AATATT 1 cut(s) 710
SspMI CTAG 1 cut(s) 888
StuI AGGCCT 1 cut(s) 1039
StyD4I CCNGG 1 cut(s) 1015
TaaI ACNGT 1 cut(s) 548
TaqI TCGA 4 cut(s) 17, 184, 292, 1074
TfiI GAWTC 4 cut(s) 200, 440, 719, 1020
TspDTI ATGAA 5 cut(s) 186, 414, 861, 946, 1082
Van91I CCANNNNNTGG 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 59
VspI ATTAAT 2 cut(s) 45, 900
XapI RAATTY 7 cut(s) 137, 509, 648, 775, 801, 1001, 1145
XceI RCATGY 1 cut(s) 972
XspI CTAG 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.