AT5G39270

Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001146
Physical Location & Seq
Reverse (-)
15729037 .. 15730231
1195 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G39270.1

Sequence Viewer

Length: 840 bp
ATGAAACTCTTAGAAAAAATGATATATGTAGAGTTTTTGATGATAATAATGGTGATATGGGTCGTGCCCATGAGTTACGGTCATGGAGCGATGATTGGTAATGCAGTCGAAGCACCTGATGTAGCGGAAGCACCAGGGATTAATGACCCAAGCAAGGCACTCGACACTAATTGGTATGACGCACGAGCCACGTTCTACGGTGACATCCATGGTGGCGACACTCAACAGGGAGCTTGTGGATACGGTAATCTATTCAGACAAGGCTATGGTCTAGCCACCGCAGCGTTAAGTACGGCACTCTTCAACGACGGGTACACATGTGGGGCTTGTTATGAAATCATGTGCACGCGTGATCCACAATGGTGTTTACCCGGATCCGTCAAGATCACAGCAACAAACTTCTGTCCCGCAAATTACAGCAAAACCACAGACCTTTGGTGCAACCCACCACAGAAACACTTTGATCTCTCCTTAGCAATGTTTCTCAAGATTGCCAAGTACAAAGCCGGGGTTGTCCCAGTTAGATACAGACGTATTCCTTGTAGCAAAACTGGTGGTGTCAAGTTTGAAACCAAAGGAAACCCTTATTTCTTAATGGTCTTGATATACAACGTAGGAGGAGCTGGAGATATTAAGTACGTGCAAGTTAAAGGAAACAAGACCGGGTGGATAACAATGAAAAAGAACTGGGGGCAGAATTGGACCACTATTACAGTATTGACCGGCCAAGGTTTATCGTTCAGGGTTACAACGAGTGATGGGATTACAAAAGATTTTTGGAATGTGATGCCAAAAAATTGGGGGTTTGGACAGACTTTTGATGGTAGGATTAACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0016020 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

279

Amino Acids

30.96

Weight (kDa)

8.9

Isoelectric Point (pI)

19.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 93 - 176 4.8e-21 Lytic transglycolase
Expansin_C PF01357 187 - 264 9e-27 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 349
AciI CCGC 3 cut(s) 125, 279, 408
AclWI GGATC 3 cut(s) 347, 369, 382
AcoI YGGCCR 1 cut(s) 724
AfaI GTAC 4 cut(s) 292, 314, 500, 638
AfiI CCNNNNNNNGG 1 cut(s) 154
AflIII ACRYGT 2 cut(s) 317, 347
AgsI TTSAA 2 cut(s) 304, 569
AjnI CCWGG 1 cut(s) 133
AleI CACNNNNGTG 1 cut(s) 361
AluBI AGCT 2 cut(s) 233, 623
AluI AGCT 2 cut(s) 233, 623
Alw21I GWGCWC 1 cut(s) 347
Alw44I GTGCAC 1 cut(s) 343
AlwI GGATC 3 cut(s) 347, 369, 382
AoxI GGCC 1 cut(s) 724
ApaLI GTGCAC 1 cut(s) 343
ApeKI GCWGC 1 cut(s) 281
AseI ATTAAT 1 cut(s) 141
AspS9I GGNCC 1 cut(s) 702
AsuC2I CCSGG 3 cut(s) 372, 508, 664
AsuHPI GGTGA 2 cut(s) 64, 212
AvaII GGWCC 1 cut(s) 702
BaeGI GKGCMC 2 cut(s) 69, 347
BaeI ACNNNNGTAYC 4 cut(s) 304, 337, 517, 550
BamHI GGATCC 1 cut(s) 374
BauI CACGAG 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 347
BbvI GCAGC 1 cut(s) 293
BccI CCATC 2 cut(s) 752, 815
BceAI ACGGC 1 cut(s) 309
BcgI CGANNNNNNTGC 2 cut(s) 142, 176
BciT130I CCWGG 1 cut(s) 135
BciVI GTATCC 1 cut(s) 233
BcnI CCSGG 3 cut(s) 372, 508, 664
BfaI CTAG 1 cut(s) 272
BfuI GTATCC 1 cut(s) 233
BisI GCNGC 1 cut(s) 282
BlsI GCNGC 1 cut(s) 283
Bme1390I CCNGG 4 cut(s) 135, 372, 508, 664
Bme18I GGWCC 1 cut(s) 702
BmgT120I GGNCC 1 cut(s) 702
BmiI GGNNCC 1 cut(s) 376
BmrFI CCNGG 4 cut(s) 135, 372, 508, 664
BmrI ACTGGG 2 cut(s) 512, 697
BmsI GCATC 1 cut(s) 777
BmuI ACTGGG 2 cut(s) 512, 697
BpmI CTGGAG 1 cut(s) 645
Bpu10I CCTNAGC 1 cut(s) 472
BpuEI CTTGAG 1 cut(s) 470
BpuMI CCSGG 3 cut(s) 372, 508, 664
BsaAI YACGTR 1 cut(s) 640
BsaJI CCNNGG 4 cut(s) 134, 208, 507, 727
Bsc4I CCNNNNNNNGG 1 cut(s) 154
Bse118I RCCGGY 1 cut(s) 722
Bse1I ACTGG 3 cut(s) 518, 556, 692
Bse3DI GCAATG 1 cut(s) 483
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 4 cut(s) 134, 208, 507, 727
BseGI GGATG 1 cut(s) 204
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMI GCAATG 1 cut(s) 483
BseNI ACTGG 3 cut(s) 518, 556, 692
BseRI GAGGAG 1 cut(s) 633
BseSI GKGCMC 2 cut(s) 69, 347
BseXI GCAGC 1 cut(s) 293
Bsh1236I CGCG 1 cut(s) 349
BshFI GGCC 1 cut(s) 726
BsiHKAI GWGCWC 1 cut(s) 347
BsiSI CCGG 4 cut(s) 372, 507, 663, 723
BslFI GGGAC 2 cut(s) 390, 500
BslI CCNNNNNNNGG 1 cut(s) 154
BsmFI GGGAC 2 cut(s) 390, 500
BsnI GGCC 1 cut(s) 726
Bsp1286I GDGCHC 2 cut(s) 69, 347
Bsp143I GATC 4 cut(s) 352, 374, 384, 463
Bsp19I CCATGG 1 cut(s) 208
BspACI CCGC 3 cut(s) 125, 279, 408
BspANI GGCC 1 cut(s) 726
BspFNI CGCG 1 cut(s) 349
BspLI GGNNCC 1 cut(s) 376
BspPI GGATC 3 cut(s) 347, 369, 382
BsrDI GCAATG 1 cut(s) 483
BsrFI RCCGGY 1 cut(s) 722
BsrI ACTGG 3 cut(s) 518, 556, 692
BssAI RCCGGY 1 cut(s) 722
BssECI CCNNGG 4 cut(s) 134, 208, 507, 727
BssMI GATC 4 cut(s) 352, 374, 384, 463
BssSI CACGAG 1 cut(s) 183
BssT1I CCWWGG 2 cut(s) 208, 727
Bst2BI CACGAG 1 cut(s) 183
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 4 cut(s) 80, 200, 245, 715
Bst6I CTCTTC 1 cut(s) 305
BstBAI YACGTR 1 cut(s) 640
BstC8I GCNNGC 1 cut(s) 347
BstDEI CTNAG 2 cut(s) 10, 472
BstDSI CCRYGG 1 cut(s) 208
BstF5I GGATG 1 cut(s) 204
BstFNI CGCG 1 cut(s) 349
BstKTI GATC 4 cut(s) 355, 377, 387, 466
BstMBI GATC 4 cut(s) 352, 374, 384, 463
BstMWI GCNNNNNNNGC 2 cut(s) 110, 281
BstNI CCWGG 1 cut(s) 135
BstNSI RCATGY 1 cut(s) 321
BstSCI CCNGG 4 cut(s) 133, 370, 506, 662
BstSLI GKGCMC 2 cut(s) 69, 347
BstUI CGCG 1 cut(s) 349
BstV1I GCAGC 1 cut(s) 293
BstX2I RGATCY 1 cut(s) 374
BstXI CCANNNNNNTGG 1 cut(s) 798
BstYI RGATCY 1 cut(s) 374
BsuI GTATCC 1 cut(s) 233
BsuRI GGCC 1 cut(s) 726
BtgI CCRYGG 1 cut(s) 208
BtgZI GCGATG 1 cut(s) 104
BtsCI GGATG 1 cut(s) 204
Cac8I GCNNGC 1 cut(s) 347
Cfr10I RCCGGY 1 cut(s) 722
Cfr13I GGNCC 1 cut(s) 702
CseI GACGC 1 cut(s) 188
Csp6I GTAC 4 cut(s) 291, 313, 499, 637
CspCI CAANNNNNGTGG 2 cut(s) 535, 570
CviAII CATG 5 cut(s) 70, 83, 209, 318, 340
CviJI RGCY 8 cut(s) 188, 233, 264, 275, 326, 506, 623, 726
CviKI_1 RGCY 8 cut(s) 188, 233, 264, 275, 326, 506, 623, 726
CviQI GTAC 4 cut(s) 291, 313, 499, 637
DdeI CTNAG 2 cut(s) 10, 472
DpnI GATC 4 cut(s) 354, 376, 386, 465
DpnII GATC 4 cut(s) 352, 374, 384, 463
EaeI YGGCCR 1 cut(s) 724
Eam1104I CTCTTC 1 cut(s) 305
EarI CTCTTC 1 cut(s) 305
Eco130I CCWWGG 2 cut(s) 208, 727
Eco47I GGWCC 1 cut(s) 702
EcoRII CCWGG 1 cut(s) 133
EcoT14I CCWWGG 2 cut(s) 208, 727
ErhI CCWWGG 2 cut(s) 208, 727
FaeI CATG 5 cut(s) 73, 86, 212, 321, 343
FaqI GGGAC 2 cut(s) 390, 500
FatI CATG 5 cut(s) 69, 82, 208, 317, 339
FauI CCCGC 1 cut(s) 415
Fnu4HI GCNGC 1 cut(s) 282
FokI GGATG 1 cut(s) 191
Fsp4HI GCNGC 1 cut(s) 282
FspBI CTAG 1 cut(s) 272
GluI GCNGC 1 cut(s) 282
GsuI CTGGAG 1 cut(s) 645
HaeIII GGCC 1 cut(s) 726
HapII CCGG 4 cut(s) 372, 507, 663, 723
HgaI GACGC 1 cut(s) 188
Hin1II CATG 5 cut(s) 73, 86, 212, 321, 343
HpaII CCGG 4 cut(s) 372, 507, 663, 723
HphI GGTGA 2 cut(s) 64, 212
Hpy166II GTNNAC 3 cut(s) 315, 345, 368
Hpy188I TCNGA 1 cut(s) 257
Hpy188III TCNNGA 3 cut(s) 382, 487, 601
Hpy8I GTNNAC 3 cut(s) 315, 345, 368
Hpy99I CGWCG 1 cut(s) 311
HpyCH4III ACNGT 4 cut(s) 80, 200, 245, 715
HpyCH4IV ACGT 4 cut(s) 191, 532, 612, 639
HpyCH4V TGCA 4 cut(s) 104, 345, 441, 643
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 281
HpyF3I CTNAG 2 cut(s) 10, 472
HpySE526I ACGT 4 cut(s) 191, 532, 612, 639
Hsp92II CATG 5 cut(s) 73, 86, 212, 321, 343
Kzo9I GATC 4 cut(s) 352, 374, 384, 463
LmnI GCTCC 3 cut(s) 86, 230, 620
Lsp1109I GCAGC 1 cut(s) 293
LweI GCATC 1 cut(s) 777
MaeI CTAG 1 cut(s) 272
MaeII ACGT 4 cut(s) 191, 532, 612, 639
MaeIII GTNAC 3 cut(s) 74, 200, 745
MalI GATC 4 cut(s) 354, 376, 386, 465
MboI GATC 4 cut(s) 352, 374, 384, 463
MboII GAAGA 1 cut(s) 292
MflI RGATCY 1 cut(s) 374
MhlI GDGCHC 2 cut(s) 69, 347
MluCI AATT 4 cut(s) 169, 412, 697, 796
MluI ACGCGT 1 cut(s) 347
MnlI CCTC 1 cut(s) 611
MseI TTAA 6 cut(s) 141, 287, 593, 633, 648, 831
MslI CAYNNNNRTG 1 cut(s) 361
MspI CCGG 4 cut(s) 372, 507, 663, 723
MspR9I CCNGG 4 cut(s) 135, 372, 508, 664
MvaI CCWGG 1 cut(s) 135
MvnI CGCG 1 cut(s) 349
MwoI GCNNNNNNNGC 2 cut(s) 110, 281
NciI CCSGG 3 cut(s) 372, 508, 664
NcoI CCATGG 1 cut(s) 208
NdeII GATC 4 cut(s) 352, 374, 384, 463
NlaIII CATG 5 cut(s) 73, 86, 212, 321, 343
NlaIV GGNNCC 1 cut(s) 376
NmuCI GTSAC 1 cut(s) 200
NspI RCATGY 1 cut(s) 321
OliI CACNNNNGTG 1 cut(s) 361
PciI ACATGT 1 cut(s) 317
PkrI GCNGC 1 cut(s) 283
Ppu21I YACGTR 1 cut(s) 640
PscI ACATGT 1 cut(s) 317
PshBI ATTAAT 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 1 cut(s) 376
PspPI GGNCC 1 cut(s) 702
PsuI RGATCY 1 cut(s) 374
RsaI GTAC 4 cut(s) 292, 314, 500, 638
RsaNI GTAC 4 cut(s) 291, 313, 499, 637
RseI CAYNNNNRTG 1 cut(s) 361
SaqAI TTAA 6 cut(s) 141, 287, 593, 633, 648, 831
SatI GCNGC 1 cut(s) 282
Sau3AI GATC 4 cut(s) 352, 374, 384, 463
Sau96I GGNCC 1 cut(s) 702
ScrFI CCNGG 4 cut(s) 135, 372, 508, 664
SduI GDGCHC 2 cut(s) 69, 347
SetI ASST 9 cut(s) 118, 194, 235, 435, 535, 615, 625, 642, 733
SfaNI GCATC 1 cut(s) 777
SinI GGWCC 1 cut(s) 702
SmiMI CAYNNNNRTG 1 cut(s) 361
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
Sse9I AATT 4 cut(s) 169, 412, 697, 796
SsiI CCGC 3 cut(s) 125, 279, 408
SspMI CTAG 1 cut(s) 272
StyD4I CCNGG 4 cut(s) 133, 370, 506, 662
StyI CCWWGG 2 cut(s) 208, 727
TaaI ACNGT 4 cut(s) 80, 200, 245, 715
TaiI ACGT 4 cut(s) 194, 535, 615, 642
TaqI TCGA 2 cut(s) 108, 162
TasI AATT 4 cut(s) 169, 412, 697, 796
TatI WGTACW 1 cut(s) 498
Tru1I TTAA 6 cut(s) 141, 287, 593, 633, 648, 831
Tru9I TTAA 6 cut(s) 141, 287, 593, 633, 648, 831
TseFI GTSAC 1 cut(s) 200
TseI GCWGC 1 cut(s) 281
Tsp45I GTSAC 1 cut(s) 200
TspDTI ATGAA 3 cut(s) 17, 348, 692
TspGWI ACGGA 1 cut(s) 367
VneI GTGCAC 1 cut(s) 343
VpaK11BI GGWCC 1 cut(s) 702
VspI ATTAAT 1 cut(s) 141
XceI RCATGY 1 cut(s) 321
XspI CTAG 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.