Rorug05G0514600

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001146
Physical Location & Seq
Reverse (-)
69990697 .. 69994544
3848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0514600.1

Sequence Viewer

Length: 777 bp
ATGGCTTTCTCTAGCAAGACCCTTTCGTTTTTTCTTGGTTTCTGGTTTTCGTTGTTGTTGCTATGGTCTCCCTCCACTTGTTCTGCTTCAGCTAGAATTTTTCTGCCTTTGGAATCTGGCAAAGTCTCCTTGGCTTTGTACTATGAGTCCCTCTGCCCCTACAGCGCTAACTTCGTGGTTAATTACTTGGTCAAGCTCTTTGAAGATGATCTAATCTCCATTGTTGATCTCAAGCTTGTGCCTTGGGGCAATGCCAAGCTCAGAGGCAATGACACCTTTGATTGCCAGCATGGTCCATCTGAATGCTTATTGAACACGGTGGAAGCCTGTGCAATTGAGACCTGGCCTGCACTGAATGATCATTTTCCTTTCATTTATTGTGTTGAGAGTTTGGTTTACGAGCACAAGTATCCCCAGTGGGAGTCATGTTATGAGAAACTGGGCCTGAACTCAACACTTATTGCTGAATGCTACAGTAGTGGACATGGCAAAGAGCTTGAACTAAAGTATGCAGCTGAAACCAATGCACTCCAGCCTCCACATCAGTATGTGCCATGGGTAGTTGTGGATGGACAGCCACTTTACGAGGACTATGAAAACTTTCTAAGCTATGTCTGCAACGCTTATAATGGCACCACAATGCTCAAGGGCTGCAATGAATACCTCAATACTATCCAAATGGCAGGGGAAAAACCTAACGATCCAGTCGCCTGCAAGGAGGGAAAGATGCCAGCATTATTTGGAAGAATTCGATCAACTCTAACTTCATGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

258

Amino Acids

29.19

Weight (kDa)

4.88

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GILT PF03227 44 - 146 7e-32 Gamma interferon inducible lysosomal thiol reductase (GILT)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 627
AccB1I GGYRCC 1 cut(s) 632
AclWI GGATC 1 cut(s) 695
AcsI RAATTY 2 cut(s) 96, 747
AcuI CTGAAG 1 cut(s) 72
AfaI GTAC 1 cut(s) 140
AfeI AGCGCT 1 cut(s) 166
AgsI TTSAA 3 cut(s) 203, 313, 500
AjnI CCWGG 1 cut(s) 341
AluBI AGCT 7 cut(s) 92, 196, 235, 259, 496, 515, 609
AluI AGCT 7 cut(s) 92, 196, 235, 259, 496, 515, 609
Alw21I GWGCWC 1 cut(s) 405
Alw26I GTCTC 3 cut(s) 72, 130, 332
AlwI GGATC 1 cut(s) 695
Aor51HI AGCGCT 1 cut(s) 166
AoxI GGCC 2 cut(s) 344, 442
ApeKI GCWGC 2 cut(s) 512, 651
ApoI RAATTY 2 cut(s) 96, 747
Asp700I GAANNNNTTC 1 cut(s) 600
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 2 cut(s) 293, 442
AvaII GGWCC 1 cut(s) 293
BanI GGYRCC 1 cut(s) 632
Bbv12I GWGCWC 1 cut(s) 405
BbvI GCAGC 2 cut(s) 524, 638
BccI CCATC 2 cut(s) 304, 563
BciT130I CCWGG 1 cut(s) 343
BciVI GTATCC 1 cut(s) 420
BclI TGATCA 1 cut(s) 358
BcoDI GTCTC 3 cut(s) 72, 130, 332
BfaI CTAG 2 cut(s) 12, 93
BfmI CTRYAG 2 cut(s) 160, 472
BfoI RGCGCY 1 cut(s) 168
BfuI GTATCC 1 cut(s) 420
BisI GCNGC 2 cut(s) 513, 652
BlsI GCNGC 2 cut(s) 514, 653
Bme1390I CCNGG 1 cut(s) 343
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 2 cut(s) 293, 442
BmiI GGNNCC 1 cut(s) 634
BmrFI CCNGG 1 cut(s) 343
BmrI ACTGGG 2 cut(s) 409, 449
BmsI GCATC 1 cut(s) 717
BmuI ACTGGG 2 cut(s) 409, 449
BpmI CTGGAG 1 cut(s) 515
BpuEI CTTGAG 2 cut(s) 215, 629
BsaI GGTCTC 2 cut(s) 72, 332
BsaJI CCNNGG 3 cut(s) 129, 242, 554
Bse1I ACTGG 3 cut(s) 415, 444, 704
Bse3DI GCAATG 3 cut(s) 256, 274, 661
BseBI CCWGG 1 cut(s) 343
BseDI CCNNGG 3 cut(s) 129, 242, 554
BseGI GGATG 2 cut(s) 574, 777
BseMI GCAATG 3 cut(s) 256, 274, 661
BseMII CTCAG 1 cut(s) 274
BseNI ACTGG 3 cut(s) 415, 444, 704
BseXI GCAGC 2 cut(s) 524, 638
BsgI GTGCAG 1 cut(s) 333
BshFI GGCC 2 cut(s) 346, 444
BshNI GGYRCC 1 cut(s) 632
BsiHKAI GWGCWC 1 cut(s) 405
BslFI GGGAC 1 cut(s) 133
BsmAI GTCTC 3 cut(s) 72, 130, 332
BsmFI GGGAC 1 cut(s) 133
BsmI GAATGC 2 cut(s) 308, 473
BsnI GGCC 2 cut(s) 346, 444
Bso31I GGTCTC 2 cut(s) 72, 332
Bsp1286I GDGCHC 1 cut(s) 405
Bsp143I GATC 5 cut(s) 208, 226, 358, 700, 752
Bsp19I CCATGG 1 cut(s) 554
BspANI GGCC 2 cut(s) 346, 444
BspCNI CTCAG 1 cut(s) 273
BspLI GGNNCC 1 cut(s) 634
BspPI GGATC 1 cut(s) 695
BspT107I GGYRCC 1 cut(s) 632
BspTNI GGTCTC 2 cut(s) 72, 332
BsrDI GCAATG 3 cut(s) 256, 274, 661
BsrI ACTGG 3 cut(s) 415, 444, 704
BssECI CCNNGG 3 cut(s) 129, 242, 554
BssMI GATC 5 cut(s) 208, 226, 358, 700, 752
BssT1I CCWWGG 3 cut(s) 129, 242, 554
Bst2UI CCWGG 1 cut(s) 343
Bst4CI ACNGT 2 cut(s) 319, 476
BstC8I GCNNGC 4 cut(s) 287, 348, 712, 732
BstDEI CTNAG 2 cut(s) 260, 605
BstDSI CCRYGG 1 cut(s) 554
BstF5I GGATG 2 cut(s) 574, 777
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 5 cut(s) 211, 229, 361, 703, 755
BstMAI GTCTC 3 cut(s) 72, 130, 332
BstMBI GATC 5 cut(s) 208, 226, 358, 700, 752
BstMWI GCNNNNNNNGC 2 cut(s) 162, 615
BstNI CCWGG 1 cut(s) 343
BstSCI CCNGG 1 cut(s) 341
BstSFI CTRYAG 2 cut(s) 160, 472
BstV1I GCAGC 2 cut(s) 524, 638
BsuI GTATCC 1 cut(s) 420
BsuRI GGCC 2 cut(s) 346, 444
BtgI CCRYGG 1 cut(s) 554
BtsCI GGATG 2 cut(s) 574, 777
BtsIMutI CAGTG 2 cut(s) 350, 422
Cac8I GCNNGC 4 cut(s) 287, 348, 712, 732
CfoI GCGC 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 293, 442
Csp6I GTAC 1 cut(s) 139
CviAII CATG 5 cut(s) 290, 426, 485, 555, 768
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 2 cut(s) 260, 605
DpnI GATC 5 cut(s) 210, 228, 360, 702, 754
DpnII GATC 5 cut(s) 208, 226, 358, 700, 752
Eco130I CCWWGG 3 cut(s) 129, 242, 554
Eco31I GGTCTC 2 cut(s) 72, 332
Eco47I GGWCC 1 cut(s) 293
Eco47III AGCGCT 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 72
EcoRI GAATTC 1 cut(s) 747
EcoRII CCWGG 1 cut(s) 341
EcoT14I CCWWGG 3 cut(s) 129, 242, 554
ErhI CCWWGG 3 cut(s) 129, 242, 554
FaeI CATG 5 cut(s) 293, 429, 488, 558, 771
FaqI GGGAC 1 cut(s) 133
FatI CATG 5 cut(s) 289, 425, 484, 554, 767
FbaI TGATCA 1 cut(s) 358
Fnu4HI GCNGC 2 cut(s) 513, 652
FokI GGATG 1 cut(s) 581
Fsp4HI GCNGC 2 cut(s) 513, 652
FspBI CTAG 2 cut(s) 12, 93
GlaI GCGC 1 cut(s) 166
GluI GCNGC 2 cut(s) 513, 652
GsuI CTGGAG 1 cut(s) 515
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 2 cut(s) 346, 444
HhaI GCGC 1 cut(s) 167
Hin1II CATG 5 cut(s) 293, 429, 488, 558, 771
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 3 cut(s) 113, 146, 422
Hpy166II GTNNAC 2 cut(s) 397, 482
Hpy188I TCNGA 2 cut(s) 263, 301
Hpy8I GTNNAC 2 cut(s) 397, 482
HpyCH4III ACNGT 2 cut(s) 319, 476
HpyCH4V TGCA 7 cut(s) 332, 350, 512, 527, 618, 654, 714
HpyF10VI GCNNNNNNNGC 2 cut(s) 162, 615
HpyF3I CTNAG 2 cut(s) 260, 605
Hsp92II CATG 5 cut(s) 293, 429, 488, 558, 771
HspAI GCGC 1 cut(s) 165
Ksp22I TGATCA 1 cut(s) 358
Kzo9I GATC 5 cut(s) 208, 226, 358, 700, 752
Lsp1109I GCAGC 2 cut(s) 524, 638
LweI GCATC 1 cut(s) 717
MaeI CTAG 2 cut(s) 12, 93
MalI GATC 5 cut(s) 210, 228, 360, 702, 754
MboI GATC 5 cut(s) 208, 226, 358, 700, 752
MboII GAAGA 2 cut(s) 215, 756
MfeI CAATTG 1 cut(s) 333
MhlI GDGCHC 1 cut(s) 405
MluCI AATT 4 cut(s) 96, 181, 333, 747
MlyI GAGTC 2 cut(s) 155, 431
MnlI CCTC 7 cut(s) 82, 161, 257, 546, 580, 674, 712
MroXI GAANNNNTTC 1 cut(s) 600
MseI TTAA 1 cut(s) 180
MslI CAYNNNNRTG 4 cut(s) 301, 546, 638, 772
MspA1I CMGCKG 1 cut(s) 515
MspR9I CCNGG 1 cut(s) 343
MunI CAATTG 1 cut(s) 333
Mva1269I GAATGC 2 cut(s) 308, 473
MvaI CCWGG 1 cut(s) 343
MwoI GCNNNNNNNGC 2 cut(s) 162, 615
NcoI CCATGG 1 cut(s) 554
NdeII GATC 5 cut(s) 208, 226, 358, 700, 752
NlaIII CATG 5 cut(s) 293, 429, 488, 558, 771
NlaIV GGNNCC 1 cut(s) 634
PctI GAATGC 2 cut(s) 308, 473
PdmI GAANNNNTTC 1 cut(s) 600
PfeI GAWTC 1 cut(s) 113
PkrI GCNGC 2 cut(s) 514, 653
PleI GAGTC 2 cut(s) 154, 430
PpsI GAGTC 2 cut(s) 154, 430
PsiI TTATAA 1 cut(s) 627
Psp6I CCWGG 1 cut(s) 341
PspGI CCWGG 1 cut(s) 341
PspN4I GGNNCC 1 cut(s) 634
PspPI GGNCC 2 cut(s) 293, 442
PvuII CAGCTG 1 cut(s) 515
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
RseI CAYNNNNRTG 4 cut(s) 301, 546, 638, 772
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 2 cut(s) 513, 652
Sau3AI GATC 5 cut(s) 208, 226, 358, 700, 752
Sau96I GGNCC 2 cut(s) 293, 442
SchI GAGTC 2 cut(s) 155, 431
ScrFI CCNGG 1 cut(s) 343
SduI GDGCHC 1 cut(s) 405
SfaNI GCATC 1 cut(s) 717
SfcI CTRYAG 2 cut(s) 160, 472
SinI GGWCC 1 cut(s) 293
SmiMI CAYNNNNRTG 4 cut(s) 301, 546, 638, 772
SmlI CTYRAG 2 cut(s) 230, 644
SmoI CTYRAG 2 cut(s) 230, 644
Sse9I AATT 4 cut(s) 96, 181, 333, 747
SspMI CTAG 2 cut(s) 12, 93
StyD4I CCNGG 1 cut(s) 341
StyI CCWWGG 3 cut(s) 129, 242, 554
TaaI ACNGT 2 cut(s) 319, 476
TaqI TCGA 1 cut(s) 751
TasI AATT 4 cut(s) 96, 181, 333, 747
TatI WGTACW 1 cut(s) 138
TfiI GAWTC 1 cut(s) 113
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TscAI CASTG 2 cut(s) 357, 422
TseI GCWGC 2 cut(s) 512, 651
TspDTI ATGAA 4 cut(s) 361, 609, 672, 756
TspRI CASTG 2 cut(s) 357, 422
VpaK11BI GGWCC 1 cut(s) 293
XapI RAATTY 2 cut(s) 96, 747
XmnI GAANNNNTTC 1 cut(s) 600
XspI CTAG 2 cut(s) 12, 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.